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Nikitovic, Dragana, Katerina Kouvidi, Nikos K. Karamanos, and George N. Tzanakakis. "The Roles of Hyaluronan/RHAMM/CD44 and Their Respective Interactions along the Insidious Pathways of Fibrosarcoma Progression." BioMed Research International 2013 (2013): 1–12. http://dx.doi.org/10.1155/2013/929531.
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Fibrosarcomas are rare malignant mesenchymal tumors originating from fibroblasts. Importantly, fibrosarcoma cells were shown to have a high content and turnover of extracellular matrix (ECM) components including hyaluronan (HA), proteoglycans, collagens, fibronectin, and laminin. ECMs are complicated structures that surround and support cells within tissues. During cancer progression, significant changes can be observed in the structural and mechanical properties of the ECM components. Importantly, hyaluronan deposition is usually higher in malignant tumors as compared to benign tissues, predicting tumor progression in some tumor types. Furthermore, activated stromal cells are able to produce tissue structure rich in hyaluronan in order to promote tumor growth. Key biological roles of HA result from its interactions with its specific CD44 and RHAMM (receptor for HA-mediated motility) cell-surface receptors. HA-receptor downstream signaling pathways regulate in turn cellular processes implicated in tumorigenesis. Growth factors, including PDGF-BB, TGFβ2, and FGF-2, enhanced hyaluronan deposition to ECM and modulated HA-receptor expression in fibrosarcoma cells. Indeed, FGF-2 through upregulation of specific HAS isoforms and hyaluronan synthesis regulated secretion and net hyaluronan deposition to the fibrosarcoma pericellular matrix modulating these cells’ migration capability. In this paper we discuss the involvement of hyaluronan/RHAMM/CD44 mediated signaling in the insidious pathways of fibrosarcoma progression.
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Valdèz, J. C., M. Rachid, N. Gobbato, and G. Perdigòn. "Apoptosis Study in Thymic Involution in Tumour-Bearing Mice." International Journal of Immunopathology and Pharmacology 11, no. 2 (May 1998): 49–55. http://dx.doi.org/10.1177/039463209801100201.
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By using a colorectal carcinoma induced by s.c. injection of 1,2-dimethylhydrazine and a transplantable fibrosarcoma developed in inbred BALB/c mice 6–8 weeks old we found that tumour development was accompanied by a remarkable thymic involution. Mice bearing small fibrosarcoma or carcinoma (0,05 cm3) had thymuses and spleens with the same weight as those of normal mice. Thymic atrophy and splenomegalia developed in mice bearing large fibrosarcoma (5,00 cm3) and large carcinoma (0,20 cm3). Thymic involution was not the result of an increase in spontaneous cellular apoptosis. However, an increased susceptibility to apoptosis induced by etoposide was observed in thymocytes from mice bearing large carcinomas or large fibrosarcomas, as compared to the same cells derived from normal or small tumour-bearing mice. Dexamethasone induced comparable levels of apoptosis in thymocytes from all groups (normal mice, mice bearing small and large carcinoma and mice bearing small and large fibrosarcoma); doxorrubicin had no significant effect in any group.
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VP, Vinodh, Rahmat Harun, Pulivendhan Sellamuthu, and Regunath Kandasamy. "Primary Central Nervous System Fibrosarcoma." Journal of Neurosciences in Rural Practice 08, S 01 (August 2017): S111—S113. http://dx.doi.org/10.4103/jnrp.jnrp_165_17.
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ABSTRACTWe report a rare case of a young female with primary brain fibrosarcoma, and to the best of our knowledge, we believe that only <50 cases have been reported or described worldwide so far. Fibrosarcoma is a malignant neoplasm, in which histologically the predominant cells are fibroblasts that divide excessively without cellular control and they can invade local tissues or metastasize. Primary central nervous system fibrosarcomas are very aggressive neoplasms and generally have a poor prognosis. This tumor is either from sarcomatous transformation of a meningioma or arises de novo within the brain parenchyma. Our patient, a 48-year-old woman, who presented with progressive speech disorder over the period of 4 months, showed a left temporoparietal lesion with surrounding edema and local mass effect. Total surgical resection was achieved. Histopathology revealed classical fibrosarcoma features and secondary screening revealed no other distant lesion as diagnosis of primary brain fibrosarcoma was established. This case is deemed to be extremely rare because most reports claim that recurrence is within 6 months with poor prognosis; however, this patient is currently recurrence-free at 3 years. This would suggest of the possibility for a relook into this disease's course and recurrence rate when complete excision is achieved. Due to extreme rarity of these tumors, more comparative studies will be needed to improve the disease outcome.
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Vysotskaya, O. V., A. I. Glukhov, Yu P. Semochkina, S. A. Gordeev, and E. Yu Moskaleva. "Telomerase activity, mTert gene expression and the telomere length in mouse mesenchymal stem cells in the late period after γ- and γ,n-irradiation and in the tumors developed from these cells." Biomeditsinskaya Khimiya 66, no. 3 (2020): 265–73. http://dx.doi.org/10.18097/pbmc20206603265.
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In proliferating normal and tumor cells, the telomere length (TL) is maintained by high telomerase activity (TA). In the absence of TA the TL maintenance involves a mechanism of alternative lengthening of telomeres (ALT). The aim of this study was to investigate the level of TA, the mTert expression and TL in cultured normal and transformed by γ- and γ,n-irradiation mesenchymal stem cells (MSCs) from mouse bone marrow, in sarcomas that developed after the transplantation of these cells into syngeneic mice, and in fibrosarcoma cell lines obtained from these tumors to find out the role of AT or ALT in maintaining TL in these cells. During prolonged cultivation of normal and transformed under the influence of γ- (1 Gy and 6 Gy) and γ,n-irradiation (0.05 Gy, 0.5 Gy, and 2 Gy) MSCs from mouse bone marrow, a decrease in TA was detected in irradiated cells. Even deeper decrease in TA was found in sarcomas developed after administration of transformed MSCs to syngeneic mice and in fibrosarcoma cell lines isolated from these tumors in which TA was either absent or was found to be at a very low level. TL in three of the four lines obtained was halved compared to the initial MSCs. With absent or low TA and reduced TL, the cells of all the obtained fibrosarcoma lines successfully proliferated without signs of a change in survival. The mechanism of telomere maintainance in fibrosarcoma cell lines in the absence of TA needs further investigation and it can be assumed that it is associated with the use of the ALT. The detected decrease or absence of TA in transformed under the action of irradiation MSCs with the preservation or even an increase in the telomerase gene expression may be associated with the formation of inactive splicing variants, and requires further study. The obtained lines of transformed MSCs and fibrosarcomas with TA and without the activity of this enzyme can be a useful model for studying the efficacy of TA and ALT inhibitors in vitro and in vivo.
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Annese, Tiziana, Roberto Ronca, Roberto Tamma, Arianna Giacomini, Simona Ruggieri, Elisabetta Grillo, Marco Presta, and Domenico Ribatti. "PTX3 Modulates Neovascularization and Immune Inflammatory Infiltrate in a Murine Model of Fibrosarcoma." International Journal of Molecular Sciences 20, no. 18 (September 17, 2019): 4599. http://dx.doi.org/10.3390/ijms20184599.
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Fibrosarcoma is an aggressive subtype of soft tissue sarcoma categorized in infantile/congenital-type and adult-type. Fibrosarcoma cells and its surrounding immune inflammatory infiltrates overexpress or induce the expression of fibroblast growth factor-2 (FGF-2) that have a crucial role in tumor progression and angiogenesis. The inflammation-associated long pentraxin 3 (PTX3) was found to reduce FGF-2-mediated angiogenesis, but its role on fibrosarcoma immune inflammatory infiltrate is still unknown. In this study, we have evaluated the PTX3 activity on immune infiltrating mast cells, macrophages and T-lymphocytes by immunohistochemistry on murine MC-TGS17-51 fibrosarcoma cells and on transgenic TgN(Tie2-hPTX3) mouse. In these fibrosarcoma models we found a reduced neovascularization and a significant decrease of inflammatory infiltrate. Indeed, we show that PTX3 reduces the level of complement 3 (C3) deposition reducing fibrosarcoma progression. In conclusion, we hypothesize that targeting fibrosarcoma microenvironment by FGF/FGFR inhibitors may improve treatment outcome.
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&NA;. "Modified fibrosarcoma cells induce immunity." Inpharma Weekly &NA;, no. 974 (February 1995): 11. http://dx.doi.org/10.2165/00128413-199509740-00024.
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Paddock, S. W., and G. A. Dunn. "Analysing collisions between fibroblasts and fibrosarcoma cells: fibrosarcoma cells show an active invasionary response." Journal of Cell Science 81, no. 1 (March 1, 1986): 163–87. http://dx.doi.org/10.1242/jcs.81.1.163.
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We describe a direct way of measuring contact inhibition of locomotion by analysing the changes in motion of pairs of colliding cells. This allows values to be assigned to each type of cell in mixed collisions and will enable certain hypotheses about the relationship between contact inhibition and invasion in culture to be tested critically. We find that fibrosarcoma (FS9) cells, on colliding with chick heart fibroblasts, show a reversed contact-inhibition response that we call contact promotion of locomotion. We also describe a measure of the lateral changes in motion that result from collisions between cells and show that this is dependent on the type of colliding cell but, unlike contact inhibition, it does not appear to be dependent on the type of cell with which it collides for the types studied here. Finally, we analyse how the total response is dependent on the dispositions and motions of the cells before collision and we find that FS9 cells, on colliding with fibroblasts, tend to turn towards the point of initial marginal contact. We conclude that the FS9 cells show a pronounced response on colliding with the fibroblasts, which is in contrast to the subjective impression that the FS9 cells do not respond much. These findings support the thesis of Abercrombie and colleagues, that the infiltration of a population of normal cells by a population of invasive cells in culture is dependent on the nature of the response of each cell type to collision with the other and that the invasive cells fail to show contact inhibition in these heterotypic collisions; but the findings further suggest that these invasive cells show an active invasionary response as opposed to merely failing to show contact inhibition.
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Bist, SS, Sarita Mishra, Vinish Agrawal, and Meena Harsh. "Soft Tissue Fibrosarcoma Neck Mimicking as Thyroid Swelling." An International Journal of Otorhinolaryngology Clinics 6, no. 1 (2014): 50–52. http://dx.doi.org/10.5005/jp-journals-10003-1150.
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ABSTRACT Fibrosarcomas are relatively uncommon tumors, commonly arise in the extremities; approximately 10% occur in the head and neck region, most commonly in the sinonasal tract and neck. We hereby report a case of fibrosarcoma in neck clinically mimicking as a thyroid swelling in a 14 years old boy. The patient reported with difficulty in breathing along with stridor at the time of presentation so endotracheal intubation was done to secure the airway. Subsequent ultrasonography guided fine needle aspiration cytology (FNAC) showed atypical cells suggestive of mesenchymal origin. Contrast-enhanced computed tomography scan showed a large heterogeneously enhancing mass lesion in right side of neck with retrosternal extension, while the right lobe of thyroid was displaced superiorly and left lobe was normal. We performed a complete surgical excision of the tumor and histopathological examination showed intermediate to high grade spindle cell sarcoma, favoring fibrosarcoma. Postoperative period was uneventful and the patient was referred to oncology unit for radiotherapy and chemotherapy, but the patient succumbed to the disease 5 weeks after surgery. How to cite this article Bist SS, Mishra S, Varshney S, Agrawal V, Harsh M. Soft Tissue Fibrosarcoma Neck Mimicking as Thyroid Swelling. Int J Otorhinolaryngol Clin 2014; 6(1):50-52.
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Eikelberg, Deborah Johanna, Lisa Allnoch, Pierre Grothmann, Julia Bohner, and Marion Hewicker-Trautwein. "Subcutaneous fibrosarcomas with pulmonary metastases in a white tiger (Panthera tigris) and a lion (Panthera leo)." Veterinary Record Case Reports 8, no. 2 (April 2020): e000960. http://dx.doi.org/10.1136/vetreccr-2019-000960.
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Two cases of recurrent subcutaneous fibrosarcomas in a white tiger and a lion were observed and the animals were euthanised humanely due to clinical deterioration. In both animals, postmortem examination revealed multinodular, white to fawn, firm to greasy, subcutaneous masses at the left side of the thorax infiltrating into the adjacent musculature. Furthermore, the tiger showed a single mass and the lion multiple masses in the lung. Histopathologically, the subcutaneous and pulmonary masses consisted of spindle-shaped neoplastic cells with necrotic areas, and infiltration with multinucleated giant cells and lymphocytes. Immunohistochemically, tumour cells labelled positive for vimentin and negative for desmin, factor VIII-related antigen, smooth muscle actin S100, CD31 and nerve growth factor receptor p75. Thus, the pulmonary tumours were diagnosed as metastases of subcutaneous fibrosarcomas. Like domestic cats, also large, non-domestic felids could be predisposed for metastasising fibrosarcoma, which may be associated with injections or trauma.
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Kaya, Mitsunori, Takuro Wada, Satoshi Nagoya, Satoshi Kawaguchi, Toshihiko Yamashita, Nobuyuki Yamamoto, Mitsunori Yoshimoto, Futoshi Okada, and Seiichi Ishii. "TNP-470 Suppresses the Tumorigenicity of HT1080 Fibrosarcoma Tumor Through the Inhibition of VEGF Secretion From the Tumor Cells." Sarcoma 5, no. 4 (2001): 197–202. http://dx.doi.org/10.1080/13577140120099182.
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Angiogenesis inhibitors are a novel class of promising therapeutic agents for treating cancer. TNP-470, a systemic analogue of fumagillin, is an angiogenesis inhibitor capable of suppressing the tumorigenicity in several animal models even though the mechanisms of action have not been completely clarified. In the current study, we investigated the effects of TNP-470 on human fibrosarcoma cellsin vivoandin vitro. The administration of TNP-470 could suppress the tumorigenicity of HT1080 fibrosarcoma tumor. The conditioned medium from HT1080 fibrosarcoma cells treated with TNP-470 inhibited the proliferation and migration of human endothelial cell line, HUVEC and ECV304. The concentration of VEGF in the conditioned medium from HT1080 cells treated with TNP-470 was lower than that of the cells without TNP-470 treatment, indicating that TNP-470 downregulates the secretion of VEGF from HT1080 cells. These findings strongly suggest that the direct action of TNP-470 on sarcoma cells inhibits angiogenesis through the downregulation of VEGF secretion and this angiogenesis suppression resulted in the inhibition of tumorigenicity of HT1080 fibrosarcoma tumo.
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Logrono, Roberto, Ewa A. Filipowicz, Eduardo J. Eyzaguirre, and Ravindranauth N. Sawh. "Diagnosis of Primary Fibrosarcoma of the Lung by Fine-Needle Aspiration and Core Biopsy." Archives of Pathology & Laboratory Medicine 123, no. 8 (August 1, 1999): 731–35. http://dx.doi.org/10.5858/1999-123-0731-dopfot.
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Abstract Primary pulmonary sarcomas are uncommon neoplasms. Primary fibrosarcoma of the lung is extremely rare, and only 53 cases have been documented in the literature to date. To our knowledge, the diagnosis of primary lung fibrosarcoma by fine-needle aspiration cytology has never been reported. We report a case of pulmonary fibrosarcoma diagnosed by fine-needle aspiration cytology and core biopsy. The neoplasm consisted of interweaving fascicles of minimally atypical spindle cells with slender nuclei and scant cytoplasm. Positive immunohistochemistry for vimentin along with nonreactivity of tumor cells for keratin, S100 protein, desmin, α-smooth muscle actin, and CD34 supported the the diagnosis. The diagnosis was later confirmed by histologic and ultrastructural findings following lobectomy. A meticulous clinical search for a possible primary neoplasm elsewhere was unsuccessful, and lung was established as the primary site. Fine-needle aspiration cytology and core biopsy are reliable methods for establishing a diagnosis of fibrosarcoma.
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Patruno, Rosa, Giuseppe Passantino, Carmelo Laface, Antonella Tinelli, Alfredo Zito, Roberta Ruggieri, Francesco Luposella, et al. "Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma." Cells 10, no. 1 (December 28, 2020): 31. http://dx.doi.org/10.3390/cells10010031.
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Soft tissue sarcomas are a large group of different tumor types both in humans and in animals. Among them, fibrosarcoma is the most frequent malignant mesenchymal tumoral form in cats, representing up to 28% of all cat skin tumors, while human fibrosarcoma, fortunately, only represents 5% of all sarcomas and 0.025% of the world-wide burden of tumors. This low incidence in humans leads to consideration of this group of tumoral diseases as rare, so therapeutic options are few due to the difficulty of starting clinical trials. In this context, the identification of research models for fibrosarcomas could be of great interest to deepen knowledge in this field and recognize new or possible biological pathways involved in tumor progression and metastasis. Angiogenesis is considered a fundamental scattering cause of tumor aggressiveness and progression in all forms of cancer, but only a few research parameters were developed and reported to express them quantitatively and qualitatively. The role in angiogenesis of microenvironmental stromal cells, such as fibroblasts, lymphocytes, mast cells, and macrophages, was largely demonstrated since this topic was first approached, while quantification of new vessels and their blood capacity in tumoral area is a relatively recent approach that could be well developed thanks to expertise in immunohistochemistry and image analysis. In this paper, a crossing study evaluating microvascular density (MVD), endothelial area (EA), and Ki-67 proliferative index was reported for a series of formalin-fixed and paraffin-embedded tissue samples from 99 cat patients, affected by cat post-injection fibrosarcoma, by using a till ×400 magnification light microscopy. We aim to demonstrate that cat pets may be considered a useful animal model for better studying the correspondent human diseases and we report, for the first time to our knowledge, experimental data in terms of correlation among MVD, EA, and Ki-67 strictly involved in aggressiveness and tumoral progression.
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Ahmed, Ishtiaq. "Proliferating Cell Nuclear Antigen Expression in Canine and Feline Spontaneous and Injection-site Fibrosarcomas." Pakistan Veterinary Journal 40, no. 04 (December 1, 2020): 531–33. http://dx.doi.org/10.29261/pakvetj/2020.019.
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Fibrosarcoma, a tumor composed of spindloid fibroblasts, is grouped in soft tissue sarcomas which constitute one of the most important tumors in companion animals. Cell proliferation index is a good indicator of the biological behavior of the tumors which is estimated either by mitotic index or cell proliferation markers. In the current study, we investigated the immunohistochemical expression of proliferating cell nuclear antigen (PCNA) in spontaneous and injection-site fibrosarcomas in dogs and cats. A positive correlation was noticed between the PCNA expression, mitotic index, tumor grade and degree of differentiation of the tumor cells in tumors from both of these species. PCNA expression was significantly different between different tumor grades in dogs and cats. It can be concluded from this study that PCNA is a useful marker for predicting the outcome of the canine and feline fibrosarcomas
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Madej, Janusz A., Jan P. Madej, Piotr Dzięgiel, Bartosz Puła, and Marcin Nowak. "Correlation between expressions of hypoxia -inducible factor (HIF-1α), blood vessels density, cell proliferation, and apoptosis intensity in canine fibromas and fibrosarcomas." Bulletin of the Veterinary Institute in Pulawy 58, no. 1 (March 1, 2014): 117–23. http://dx.doi.org/10.2478/bvip-2014-0019.
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Abstract The study aimed to demonstrate the expression of hypoxia-inducible factor (HIF-1α) in soft tissue mesenchymal tumours (fibroma and fibrosarcoma) in dogs. An attempt was made to correlate the obtained results with density of blood vessels (expression of von Willebrand Factor, vWF), expression of Ki-67 proliferation antigen, and with intensity of apoptosis in studied tumours. The study was performed on paraffin sections of 15 fibromas and 40 fibrosarcomas sampled from 55 female dogs aged 6 to 16 years. Immunohistochemical staining against HIF-1α, vWF, and Ki-67 was performed. Apoptosis was detected with the use of TUNEL reaction. A significantly higher HIF-1α expression was noted in fibrosarcomas in comparison to fibromas (P < 0.0001). HIF-1α expression in fibromas manifested strong positive correlation with tumour vascularity (r = 0.67, P = 0.007). Moreover, HIF-1α expression in fibrosarcomas manifested a moderate positive correlation with tumour malignancy grade (r = 0.44, P = 0.004), tumour vascularity (r = 0.52, P < 0.001), Ki-67 antigen expression (r = 0.42; P = 0.007), and TUNELpositive cells (r = 0.37, P = 0.017). Expression of HIF-1α was detected in 86.7% of fibroma type tumours and in 100% of fibrosarcomas. In all studied tumours expression of HIF-1α manifested positive correlation with the density of blood vessels, and in fibrosarcomas it correlated also with malignancy grade, intensity of Ki-67 expression, and with intensity of apoptosis in tumour cells.
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Varlamova, E. G., M. V. Goltyaev, and E. E. Fesenko. "Protein-partners of selenoprotein SELM and the role of selenium compounds in regulation of its expression in human cancer cells." Доклады Академии наук 488, no. 2 (September 24, 2019): 212–16. http://dx.doi.org/10.31857/s0869-56524882212-216.
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The search of potential partners of human SELM in lysates of two cancer cell lines HT-1080 (fibrosarcoma) and MCF-7 (breast adenocarcinoma) was carried out. Two cytoplasmic actin isoforms: cytoplasmic actin 1 (cytoskeleton b-actin) and cytoplasmic actin 2 (cytoskeletal g-actin) was identified as partners. In addition, the influence of two widely used antitumor selenium compounds (sodium selenite and methylseleninic acid) on the expression SELM in cancer cells was studied. According to the results obtained by real-time PCR and Western blotting, we was concluded that 1 µM and 10 µM sodium selenite was not affected on the expression SELM in fibrosarcoma cells, whereas in breast adenocarcinoma cells 1 µM sodium selenite slightly increased of expression and 10 µM resulted in a significant decrease (about 2 times). Methylseleninic acid in both cancer cell lines increased the expression of SELM gene, the most pronounced effect was observed when fibrosarcoma cells were treated with 10 µM MSC (increased expression of the hSelm gene by almost 4 times).
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Yuwanati, Monal B., and J. V. Tupkari. "Fibrosarcoma of Mandible: A Case Report." Case Reports in Dentistry 2011 (2011): 1–4. http://dx.doi.org/10.1155/2011/536086.
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Fibrosarcoma is a malignant mesenchymal neoplasm of fibroblasts that rarely affects the oral cavity and can cause local recurrences or metastasis. In this paper, a case of fibrosarcoma in the posterior area of mandible in a 44-year-old woman is described. Clinical examination revealed a growth on right mandibular third molar region extending on the buccal and the lingual side. There was history of extraction of posterior teeth. Radiologically, there was a diffuse bone loss. Microscopically, the tumor showed an intense proliferation of fibroblasts of variable size and shape. These cells were arranged in parallel bands and partly crossing each other. The cells exhibited increased mitotic activity and nuclear pleomorphism. Immunohistochemically the cells showed immunoreactivity only for vimentin while they exhibited negativity towards S-100 protein, cytokeratin cocktail, HMB-45, desmin, smooth muscle actin, and epithelial membrane antigen (EMA). Based on findings the final diagnosis of fibrosarcoma was made.
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Longquan, Xiang, and Henry Mwakyoma. "SCLEROSING EPITHELIOID FIBROSARCOMA." Professional Medical Journal 22, no. 03 (March 10, 2015): 370–73. http://dx.doi.org/10.29309/tpmj/2015.22.03.1359.
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Sclerosing epithelioid fibrosarcoma (SEF) is a rare subtype of Fibrosarcomaand was first reported in 1995. This tumour is rarely encountered and can easily be mistaken byclinicians for other diagnoses. This paper reports one case of SEF and review related literature.Objectives: To determine the clinical pathologic characteristics and discuss differentialdiagnosis of sclerosing epithelioid fibrosarcoma (SEF). Methods: One case of SEF was studiedby clinical analysis, light microscopy and the review of the literature. Results: The patient wasan adult and had a tumor located in the subcutaneous tissue of the left leg, which waspainless and grew slowly. Macroscopically, it was described as nodular and non-encapsulated.The cut section was gray-white, firm to elastic in consistency. The microscopic examinationshowed that the round to ovoid epithelioid cells with clear or eosinophilic cytoplasm arrangedin cords, nests, sheets or alveolar pattern. The stroma showed a dense hyalinized collagenousbackground. Conclusions: Sclerosing epithelioid fibrosarcoma is a low-grade variant offibrosarcoma, histologically, it should be differentiated from a variety of tumors which have theepithelioid appearance and sclerosing stroma.
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Hansen, Tyler D., Justin T. Koepsel, Ngoc Nhi Le, Eric H. Nguyen, Stefan Zorn, Matthew Parlato, Samuel G. Loveland, Michael P. Schwartz, and William L. Murphy. "Biomaterial arrays with defined adhesion ligand densities and matrix stiffness identify distinct phenotypes for tumorigenic and non-tumorigenic human mesenchymal cell types." Biomater. Sci. 2, no. 5 (2014): 745–56. http://dx.doi.org/10.1039/c3bm60278h.
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Lou, Zhenjun, Sandra O'Reilly, Hongyan Liang, Veronica M. Maher, Stuart D. Sleight, and J. Justin McCormick. "Down-Regulation of Overexpressed Sp1 Protein in Human Fibrosarcoma Cell Lines Inhibits Tumor Formation." Cancer Research 65, no. 3 (February 1, 2005): 1007–17. http://dx.doi.org/10.1158/0008-5472.1007.65.3.
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Abstract Sp1 is a transcription factor for many genes, including genes involved in tumorigenesis. We found that human fibroblast cells malignantly transformed in culture by a carcinogen or by stable transfection of an oncogene express Sp1 at 8-fold to 18-fold higher levels than their parental cells. These cell lines form fibrosarcomas in athymic mice with a very short latency, and the cells from the tumors express the same high levels of Sp1. Similar high levels of Sp1 were found in the patient-derived fibrosarcoma cell lines tested, and in the tumors formed in athymic mice by these cell lines. To investigate the role of overexpression of Sp1 in malignant transformation of human fibroblasts, we transfected an Sp1 U1snRNA/Ribozyme into two human cell lines, malignantly transformed in culture by a carcinogen or overexpression of an oncogene, and into a patient-derived fibrosarcoma cell line. The level of expression of Sp1 in these transfected cell lines was reduced to near normal. The cells regained the spindle-shaped morphology and exhibited increased apoptosis and decreased expression of several genes linked to cancer, i.e., epithelial growth factor receptor, urokinase plasminogen activator, urokinase plasminogen activator receptor, and vascular endothelial growth factor. When injected into athymic mice, these cell lines with near normal levels of Sp1 failed to form tumors or did so only at a greatly reduced frequency and with a much longer latency. These data indicate that overexpression of Sp1 plays a causal role in malignant transformation of human fibroblasts and suggest that for cancers in which it is overexpressed, Sp1 constitutes a target for therapy.
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Sofyan, Miyayu Soneta, Koesnoto Supranianondo, Ira Sari Yudaniayanti, Aisyah Novasari, and Endah Paraswati. "Fibrosarcoma pada anjing golden retriever di Rumah Sakit Hewan Pendidikan Universitas Airlangga." ARSHI Veterinary Letters 4, no. 2 (September 20, 2020): 33–34. http://dx.doi.org/10.29244/avl.4.2.33-34.
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The dog named Agra (Golden Retriever) is suspected of having an axillary tumor. After surgery and examination of the pathology laboratory, it was found that the dog had fibrosarcoma in the axillaries. Fibrosarcoma is a malignant neoplasm that originates from mesenchymal cells, where the dominant cell histology is fibroblast cells that divide excessively and uncontrollably, can attack local tissues, and can move to other locations in the body (metastatic). The histopathological features of fibrosarcoma have fusiform or spindle-shaped cell fascicular growth patterns. The boundary between cells appears unclear with little cytoplasm and collagen fibers form parallel webbing. Grading histology is mainly based on the degree of cellularity, cell differentiation, mitotic features, and the amount of collagen produced by the cell necrosis. Surgery until now is the first and foremost thing in tumor therapy. Benign tumors can be removed with various surgical techniques depending on the location of the tumor. As for malignant tumors, surgery can be carried out by following other additional therapies to inhibit tumor growth.
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Sippola-Thiele, M., D. Hanahan, and P. M. Howley. "Cell-heritable stages of tumor progression in transgenic mice harboring the bovine papillomavirus type 1 genome." Molecular and Cellular Biology 9, no. 3 (March 1989): 925–34. http://dx.doi.org/10.1128/mcb.9.3.925-934.1989.
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Tumorigenesis of dermal fibroblasts in a line of transgenic mice carrying the BPV-1 genome was found to involve distinct proliferative stages. Cell cultures derived from normal skin, from benign proliferative fibromatoses, and from malignant fibrosarcomas each evidenced distinguishable, cell-heritable characteristics. The latent viral genome was transcriptionally inactive in normal-appearing skin and was activated in the dermal fibromatoses. Fibrosarcoma cells grew continuously in culture, formed domelike foci, and had a more rounded, anaplastic appearance. Independent cultures derived from the fibromatoses varied in their proliferative characteristics, which correlated well with the levels of viral gene expression. In contrast, progression to malignancy was not accompanied by a further increase in transgene activity, which strongly implicated cellular genetic changes in the later stages of tumorigenesis.
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Sippola-Thiele, M., D. Hanahan, and P. M. Howley. "Cell-heritable stages of tumor progression in transgenic mice harboring the bovine papillomavirus type 1 genome." Molecular and Cellular Biology 9, no. 3 (March 1989): 925–34. http://dx.doi.org/10.1128/mcb.9.3.925.
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Tumorigenesis of dermal fibroblasts in a line of transgenic mice carrying the BPV-1 genome was found to involve distinct proliferative stages. Cell cultures derived from normal skin, from benign proliferative fibromatoses, and from malignant fibrosarcomas each evidenced distinguishable, cell-heritable characteristics. The latent viral genome was transcriptionally inactive in normal-appearing skin and was activated in the dermal fibromatoses. Fibrosarcoma cells grew continuously in culture, formed domelike foci, and had a more rounded, anaplastic appearance. Independent cultures derived from the fibromatoses varied in their proliferative characteristics, which correlated well with the levels of viral gene expression. In contrast, progression to malignancy was not accompanied by a further increase in transgene activity, which strongly implicated cellular genetic changes in the later stages of tumorigenesis.
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Mamun, Md Talal, and Chowdhury Foyzur Rob. "Fibro Sarcoma of the Gingiva: A Rare Presentation." Journal of Bangladesh College of Physicians and Surgeons 40, no. 4 (October 16, 2022): 310–13. http://dx.doi.org/10.3329/jbcps.v40i4.60306.
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Fibrosarcoma is a rare malignant mesenchymal neoplasm’s of the fibroblasts which are represents 1% of all malignancies in the head and neck region. In this article we described a case of primary fibrosarcoma of the gingiva in right sided alvulas of the mandible in 16 years old male. Who was presented with a rapidly growing painful lump. Microscopically the tumor mass was composed with atypical proliferation of spindle cells arranged in intersecting fascicles and sterioform pattern. In immunohistochemically the cells showed immuno-reactivity for SAM, while negativity towards the others markers. On the basis of clinical histological and immunohistochemistry our case was diagnosed as a low grade primary fibrosarcoma of gingiva. We performed radical resection with wide margin as a primary treatment of choice without neck dissection. After primary treatment there is no signs of recurrence and metastasis was observed last 3 years follow up. J Bangladesh Coll Phys Surg 2022; 40: 310-313
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Singh, Arpanna, Hariom Singh, Dipanshu Kumar, Aparna Singh, and Biranchi Narayan Biswal. "Intraoral Soft Tissue Fibrosarcoma in a Four-Year-Old Child: An Unusual Case Report." Journal of Clinical Pediatric Dentistry 43, no. 6 (October 1, 2019): 408–12. http://dx.doi.org/10.17796/1053-4625-43.6.8.
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Fibrosarcoma is a rare soft tissue tumor of connective tissue origin that includes about 0.05% of all the malignancies in the head and neck region of which almost 23% is seen in the oral cavity. This paper describes a rare case of 4-year-old boy who presented with swelling on the right side of face diagnosed as soft tissue fibrosarcoma of the intraoral region. The histopathological and immunohistochemistry confirmed the diagnosis by the presence of spindle-shaped cells arranged in fascicles with mitotic figures and cellular proliferation reproducing fibroblasts. The patient was successfully treated with combination of chemotherapy and surgery with a good clinical outcome. This case report is presented to highlight the rarity of fibrosarcoma in orofacial region of children which requires special attention of pediatric dentist and should be considered as differential diagnose of soft tissue mass in orofacial region of children. Clinical and histopathological features must be correlated with immunohistochemistry in the final diagnosis in fibrosarcoma.
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Turishcheva, E. P., G. A. Ashniev, M. S. Vildanova, and E. A. Smirnova. "Endoplasmic Reticulum Stress Inducer Dithiothreitol Affects the Morphology and Motility of Cultured Human Dermal Fibroblasts and Fibrosarcome HT1080 Cell Line." Онтогенез 54, no. 5 (September 1, 2023): 341–57. http://dx.doi.org/10.31857/s0475145023050063.
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Some inducers of endoplasmic reticulum (ER) stress can affect the motility of normal and tumor cells. However, it is unknown what mechanisms mediate this effect and whether it is a consequence of ER stress. The aim of our work was to study the effect of the ER stress inducer dithiothreitol (DTT) on morphological features reflecting the locomotor properties of cells, as well as directly on the migratory properties of cultured human dermal fibroblasts and fibrosarcoma HT1080 cells. We have shown that DTT causes disruption of the organization of actin cytoskeleton in both types of cells, which is accompanied by a change in the cell surface and shape of cells, as well as a decrease in their spreading area. In addition, a decrease in the number of focal contacts was observed in dermal fibroblasts. DTT also reduced the motility of dermal fibroblasts and fibrosarcoma cells. To analyze cell motility and determine the moment of its change, we developed a method which showed that the change in the migratory properties of fibrosarcoma cells cultured with DTT began earlier than in dermal fibroblasts. Thus, activation of ER stress by DTT is accompanied by a change in the organization of the actin cytoskeleton and motility in normal and tumor human cells. Consequently, ER stress triggered by various inducers with different mechanisms of action affects the motility of normal and tumor cells, which must be taken into account when developing antitumor drugs that cause cell death through activation of ER stress.
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Simarmata, Yohanes T. R. M. R., Desi M. A. Biru, and Ni Made Restiati. "STUDI KASUS : FIBROSARCOMA PADA ANJING POMERANIAN MIX." JURNAL KAJIAN VETERINER 9, no. 1 (March 30, 2021): 35–49. http://dx.doi.org/10.35508/jkv.v9i1.3897.
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Fibrosarcoma is a malignant neoplasm of fibroblast that commonly found in middle-aged or older dogs. Breed and sex do not influence the incidence of fibrosarcoma. A 10 years old grey female mix Pomeranian with a bodyweight of 9.2 kg showed a semi-solid mass, with meat-like consistency that felt integrated with the tissue underneath. A mass with a diameter of ± 5 cm had been found in the left thigh near the anus. The other two unusual masses with smaller size (diameter ±1 cm) were also found at lateral sinister near extremities cranial of the body. A serial of diagnostic check-ups, such as physical and clinical check-up, USG, haematology, cytology, and histopathology tests was run to examine the dog condition. The USG result showed hypoechoic masses with slightly anechoic appearances. The haematology analysis showed a decrease in lymphocyte, MCV and hematocrit, meanwhile the granulocyte, MCHC and MHC showed an increasing trend. The cytology test revealed a fat-like vacuolization. Furthermore, the histology examination indicating the presence of large hyperchromic oval cells. The histopathology examination also found fibroblast cells that suspected as tumour cells with mitotic and infiltrated oval cores. Thus, the dog was diagnosed with fibrosarcoma and the prognosis was dubious. The surgery was done to remove the tumour masses.
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Yousofi Darani, Hossein, Narges Soozangar, Soliman Khorami, Fatomeh Taji, Mortaza Yousofi, and Hedayatollah Shirzad. "Hydatid Cyst Protoscolices Induce Cell Death in WEHI-164 Fibrosarcoma Cells and Inhibit the Proliferation of Baby Hamster Kidney FibroblastsIn Vitro." Journal of Parasitology Research 2012 (2012): 1–4. http://dx.doi.org/10.1155/2012/304183.
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Bothin vitroandin vivomodels have demonstrated that some parasites can interfere with tumor cell growth. The present study investigates the anticancer activity of hydatid cyst protoscolices on WEHI-164 fibrosarcoma cells and baby hamster kidney (BHK) fibroblast cellsin vitro. Those above two cell types were treated with live hydatid cyst protoscolices or left untreated for control groups. After 48 h, lactate dehydrogenase (LDH) and cell counts were assayed for both treated cells and control groups. Following treatment with hydatid cyst protoscolices, cell proliferation of both cell types was inhibited, and lysis of fibrosarcoma cells increased. Based on these results, it appears that hydatid cyst protoscolices have strong anticancer activity, and additional studies are needed to further clarify the mechanisms of this activity.
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Afroz, Nishat, Shagufta Qadri, Divya Rabindranath, and Shakeba Qadri. "Fibrosarcoma developing in the Parotid Gland: An Unusual Presentation." International Journal of Head and Neck Surgery 6, no. 2 (2015): 99–102. http://dx.doi.org/10.5005/jp-journals-10001-1233.
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ABSTRACT Primary sarcomas of the salivary glands occur rarely with few cases having been reported. Fibrosarcoma is a rare type of primary sarcoma with a poor prognosis and low life expectancy. Microscopically, these tumors resemble their counterparts encountered in other areas. They can be easily confused with spindle cell (sarcomatoid) carcinomas, myoepithelial carcinomas or malignant melanomas. We present an unusual case of a primary fibrosarcoma that arose in the right parotid gland of a 50 years male, who presented with a large nodular mass in the right preauricular region. Computed tomography (CT) scan revealed a tumor arising from the right parotid gland. A near total parotidectomy was performed and the histopathology report showed neoplastic spindle cells predominantly arranged in a ‘herring bone’ pattern. On immunohistochemistry, the tumor was positive for vimentin and was negative for pancytokeratin and S-100, which lead to a firm diagnosis of fibrosarcoma. How to cite this article Qadri S, Afroz N, Rabindranath D, Qadri S. Fibrosarcoma developing in the Parotid Gland: An Unusual Presentation. Int J Head Neck surg 2015;6(2):99-102.
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Huang, Y. C., K. F. Hsu, C. Y. Chou, Y. C. Dai, and C. C. Tzeng. "Ovarian fibrosarcoma with long-term survival: A case report." International Journal of Gynecologic Cancer 11, no. 4 (2001): 331–33. http://dx.doi.org/10.1136/ijgc-00009577-200107000-00016.
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Abstract.Huang Y-C, Hsu K-F, Chou C-Y, Dai Y-C, Tzeng C-C. Ovarian fibrosarcoma with long-term survival.Primary ovarian fibrosarcoma is an exceedingly rare malignant ovarian stromal tumor which has a poor prognosis. We report here a 46-year-old woman who suffered from irregular vaginal bleeding for 2 months. She received hysterectomy and salpingo-oophorectomy due to a provisional diagnosis of uterine and ovarian tumors. At surgery, an 8-cm ovarian solid multilobular tumor was found. Frozen section examination revealed an ovarian fibrosarcoma. She then underwent staging procedures including intraperitoneal washing, cytology, and pelvic and para-aortic lymph node sampling. Final pathologic examination revealed that the tumor exhibited densely packed spindle cells in storiform configuration with obvious increased mitotic activity. In addition, the flow cytometric study showed marked elevated percentage of tumor cells in the S phase (13.1%). After surgery, the patient received six courses of combination chemotherapy with epirubicin, ifosfamide, and dacarbazine (DTIC). The patient stood the treatment well and is free from disease 6 years later.
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Ozmen, Ozlem, Ozlem Sirin Sengoz, Harun Çinar, and Hüseyin Dolu. "Retrobulbar Fibrosarcoma in a Sheep." Acta Veterinaria 66, no. 2 (June 1, 2016): 265–70. http://dx.doi.org/10.1515/acve-2016-0023.
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Abstract This report describes a case of a retrobulbar fibrosarcoma in a 4-year-old female sheep. A big tumor was protruding from the orbit but not invading the surrounding tissue and was easily removed surgically. The grayish colored mass was 19×13×8 cm in size and hard. The surface of the tumor was irregular and ulcerated. Marked hemorrhage and necrotic areas were present across the whitish cut surface. Histopathologically, the mass was composed of spindle shaped, anaplastic pleomorphic cells. Masson’s trichrome staining revealed a collagenous matrix in the tissue. Immunohistochemically, the mass was positive for vimentin and proliferating cell nuclear antigen but negative for smooth muscle actin, desmin, glial fibrilar acidic protein and S100 protein antibodies. According to histopathological and immunohistochemical findings the tumor was diagnosed as fibrosarcoma. To the authors’ knowledge, this is the first report of ocular fibrosarcoma in a sheep.
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Rosiers, K., F. Smeets, L. Delooz, V. Saey, and M. Vanrobaeys. "A perirenal fibrosarcoma in a newborn calf." Vlaams Diergeneeskundig Tijdschrift 89, no. 3 (June 30, 2020): 162–65. http://dx.doi.org/10.21825/vdt.v89i3.16537.
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Congenital tumors are rare in cattle. In this case report, a calf with a congenital perirenal fibrosarcoma is described. A newborn Belgian Blue calf that succumbed shortly after caesarian section was submitted for necropsy at the diagnostic lab of the ARSIA (Association Régionale de Santé et d’Identification Animales). At necropsy, a hemorrhagic firm mass was found surrounding the left kidney. Histopathological examination of the mass revealed a neoplastic cell population. Additional immunohistochemical stainings were performed to identify the tumor. The majority of neoplastic cells stained positive for vimentin but were negative for neurofilaments (NFs), desmin, CD3, CD20, Von Willebrand factor and cytokeratin, indicating a mesenchymal origin. The tumor was diagnosed as a fibrosarcoma. To the authors’ knowledge, this is the first case of a congenital perirenal fibrosarcoma reported in a Belgian Blue calf in Belgium.
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Lynch, D. H., R. A. Daynes, and R. J. Hodes. "Cell-mediated immune responses to syngeneic tumors. I. Identification of two distinct CTL effector pathways which differ in antigen specificity, genetic regulation, and cell surface phenotype." Journal of Immunology 136, no. 4 (February 15, 1986): 1521–27. http://dx.doi.org/10.4049/jimmunol.136.4.1521.
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Abstract It has been demonstrated previously that draining lymph nodes (DLN) from tumor-immunized mice contain a population of lymphoid cells that are capable of differentiating into functional antitumor cytotoxic T lymphocytes (CTL) during in vitro culture. In the present studies, it was observed that DLN cells from either C57BL/10 (B10) or C3H mice that had been footpad-immunized with syngeneic tumor cells differentiated into CTL during a 4-day in vitro culture in the absence of added antigen. The specificity patterns of the CTL thus generated, however, were quite different in the two strains. DLN from B10 mice immunized with ultraviolet light-induced fibrosarcoma cells of B10 origin differentiated into CTL which were only capable of lysing target cells from the tumor used for immunization. Thus, the antitumor CTL which differentiate from B10 DLN appeared to be specific for the tumor-specific antigen (TSA) expressed by these tumor cells. In contrast, DLN from C3H mice immunized with a syngeneic ultraviolet light-induced fibrosarcoma differentiated into CTL which effectively lysed not only target cells from the immunizing tumor, but several other fibrosarcomas of both B10 and C3H origin, and which did not lyse normal nontumor targets. These C3H effectors thus appeared to be specific for a tumor-associated antigen (TAA) which is widely shared by a number of tumors. Cold target-blocking studies demonstrated that the CTL generated by C3H DLN cells contained a subpopulation of TSA-specific cells in addition to cross-reactive TAA-specific effectors. (B6 X C3H)F1 (B6C3F1) mice generated cross-reactive TAA-specific CTL in response to in vivo challenge with either B10 or C3H tumors, indicating that the ability to generate a TAA-specific CTL response behaves as a dominant trait of the responding mouse strain and not as a function of the tumor used for immunization. TSA-specific CTL and cross-reactive TAA-specific CTL were distinguishable on the basis of their cell surface phenotypes, because the TSA-specific CTL generated by B10 DLN cells were Thy-1.2+ Lyt-2.2+, whereas TAA-specific B6C3F1 CTL were Thy-1.2+ Lyt-2.2-; alloantigen-specific CTL generated from the same B6C3F1 lymph nodes were Thy-1.2+ Lyt-2.2+.(ABSTRACT TRUNCATED AT 400 WORDS)
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Corsi, Alessandro, Renata Boidrini, and Cesare Bosnian. "Congenital-Infantile Fibrosarcoma: Study of Two Cases and Review of the Literature." Tumori Journal 80, no. 5 (October 1994): 392–400. http://dx.doi.org/10.1177/030089169408000515.
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Background Congenital-infantile fibrosarcoma is a rare tumor of the pediatric age. It involves subjects under 5 years of age, and more than 200 cases have been reported in the literature. Methods The authors present the clinicopathologic findings of 2 cases and review the literature. Results Of our 2 patients, the first was a 2-years and 6-months-old female and the second a newborn male. The female presented a tumor in the retroperitoneum without recurrences or metastasis after 17 months, and the male on left foor with a recurrence after 3 months. Histologically, the tumors were mainly composed of spindle-shaped cells. Immunohistochemically, in both cases, neoplastic cells were positive for vimentin; focal positivity for muscular specific actin was present in the tumor of the female. Ultrastructurally, tumors were composed of mesenchymal cells with fibroblastic and myofibroblastic features. Flow cytometric analysis of the retroperitoneal tumor showed an aneuploid population of neoplastic cells. Conclusions Congenital-infantile fibrosarcoma should be considered a borderline tumor; its biologic behavior is better than that of adult fibrosarcoma. Histologic diagnosis is not easy; the microscopic picture may be confused with fibromatosis or with malignant mesenchymal neoplasms. Only a follow-up of many years can confirm the benignancy or malignancy of any individual tumor, even though clinico-pathologic features may be distinctive enough to permit its recognition.
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Cora, Roxana, Adrian Florin Gal, Marian Taulescu, Flaviu Tăbăran, Raluca Vidrighinescu, and Cornel Cătoi. "Description of a Feline Injection-Site Fibrosarcoma with Metastasis in the Cerebellum." Acta Veterinaria 67, no. 4 (December 20, 2017): 578–86. http://dx.doi.org/10.1515/acve-2017-0047.
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AbstractFeline injection-site sarcomas were initially described by Hendrick and Goldschmidt (1991). The link between vaccination site and sarcoma occurrence suggested the term of vaccine-associated sarcomas. Our paper describes an unusual feline injection-site fibrosarcoma with cerebellar metastasis. A 7-year-old female domestic short-hair cat was submitted to the Pathology Department (Faculty of Veterinary Medicine Cluj- Napoca, Romania) for necropsy. A subcutaneous tumor (relapse) in the interscapular region and multiple metastatic masses in the lungs, kidneys, subcutaneous tissue (scapular and thigh regions) and cerebellum were observed. Cytological, histological and immunohistochemical (for vimentin, desmin, multi-cytokeratin, α-smooth muscle actin, S100 and CD45) analyses from all tumors were performed. Cytological examination identifi ed highly pleomorphic spindle-shaped cells admixed with neoplastic multinucleated giant cells. Histologically, all neoplastic masses were composed of numerous spindle cells arranged into interlacing bundles. Extensive intratumoral areas of necrosis along with a neutrophilic infiltrate were also detected. A fibrillary material was present among neoplastic cells (green stained by Masson’s trichrome method), suggesting a collagenous structure. In all tumors assessed, immunohistochemistry showed an intense reaction only for vimentin in numerous neoplastic cells. Based on the history, gross, cytological, histological and immunohistochemical data, the final diagnosis was recurrent feline injection-site fibrosarcoma, with multiple metastases (including in the cerebellum). Overall, an uncommon case of feline injection-site fibrosarcoma has been reported. This is the first consistent record of an injection site sarcoma in a cat with cerebellar metastasis.
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Samuel, S. K., R. A. Hurta, M. A. Spearman, J. A. Wright, E. A. Turley, and A. H. Greenberg. "TGF-beta 1 stimulation of cell locomotion utilizes the hyaluronan receptor RHAMM and hyaluronan." Journal of Cell Biology 123, no. 3 (November 1, 1993): 749–58. http://dx.doi.org/10.1083/jcb.123.3.749.
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TGF-beta is a potent stimulator of motility in a variety of cell types. It has recently been shown that hyaluronan (HA) can directly promote locomotion of cells through interaction with the HA receptor RHAMM. We have investigated the role of RHAMM and HA in TGF-beta-stimulated locomotion and show that TGF-beta triggers the transcription, synthesis and membrane expression of the RHAMM receptor and the secretion of HA coincident with the induction of the locomotory response. This was demonstrated by both incubating cells with exogenous TGF-beta 1 and by stimulating the production of bioactive TGF-beta 1 in tumor cells transfected with TGF-beta 1 under the control of the metallothionein promoter. TGF-beta 1-induced locomotion was suppressed by antibodies that prevented HA/RHAMM interaction, using polyclonal antibodies to either RHAMM fusion protein or RHAMM peptides, or mAbs to purified RHAMM. Peptides corresponding to the HA-binding motif of RHAMM also suppressed TGF-beta 1-induced increases in motility rate. Spontaneous locomotion of fibrosarcoma cells was blocked by neutralizing secreted TGF-beta with panspecific TGF-beta antibodies and by inhibition of TGF-beta 1 secretion with antisense oligonucleotides. Polyclonal anti-RHAMM fusion protein antibodies and peptide from the RHAMM HA-binding motif also suppressed the spontaneous motility rate of fibrosarcoma cells. These data suggest that fibrosarcoma cell locomotion requires TGF-beta, and the pathway by which TGF-beta stimulates locomotion uses the HA receptor RHAMM and HA.
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Becker, Robert O. "Effect of Anodally Generated Silver Ions on Fibrosarcoma Cells." Electro- and Magnetobiology 11, no. 1 (January 1992): 57–65. http://dx.doi.org/10.3109/15368379209012853.
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Bakker, A., W. Biermans, B. Van Haesebroek, M. De Bie, I. Bernaert, and W. Jacob. "Contact sites in mitochondria of L929 murine fibrosarcoma cells." Micron and Microscopica Acta 22, no. 3 (January 1991): 255–56. http://dx.doi.org/10.1016/0739-6260(91)90009-o.
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Song, Byunghoo, Bokyung Kim, Se-Ha Choi, Kyo Young Song, Yang-Guk Chung, Youn-Soo Lee, and Gyeongsin Park. "Mesenchymal Stromal Cells Promote Tumor Progression in Fibrosarcoma and Gastric Cancer Cells." Korean Journal of Pathology 48, no. 3 (2014): 217. http://dx.doi.org/10.4132/koreanjpathol.2014.48.3.217.
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Jänicke, R. U., X. Y. Lin, F. H. Lee, and A. G. Porter. "Cyclin D3 sensitizes tumor cells to tumor necrosis factor-induced, c-Myc-dependent apoptosis." Molecular and Cellular Biology 16, no. 10 (October 1996): 5245–53. http://dx.doi.org/10.1128/mcb.16.10.5245.
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c-Myc is an important mediator of apoptosis in cytokine- or serum-deprived cells and sensitizes various cell types to tumor necrosis factor alpha (TNF) cytotoxicity. However, downstream mediators of c-Myc-dependent apoptosis are largely unknown. In this study, we investigated whether one or more cyclins which, like c-Myc, are important regulators of the cell cycle are involved in TNF-induced apoptosis downstream of c-Myc. Cyclin D3 and c-Myc levels in HeLa and fibrosarcoma cells correlated with sensitivity of these cells to TNF-induced apoptosis, as both proteins were highly expressed in TNF-sensitive HeLa D98 cells and HT-1080 fibrosarcoma cells but not in their TNF-resistant counterparts, HeLa H21 and SS-HT-1080 cells, respectively. All other cyclins tested were equally expressed in all tumor cell lines. Reduction in the expression of c-Myc by dexamethasone or inhibition of the transcriptional activity of c-Myc by introduction of a dominant negative form of c-Myc into TNF-sensitive HeLa D98 cells strongly suppressed the expression of cyclin D3 (but none of the other cyclins) and rendered the cells resistant to TNF-induced apoptosis. Conversely, introduction of the c-myc gene into TNF-resistant, c-Myc- and cyclin D3-deficient HeLa H21 cells resulted in enhanced cyclin D3 expression and TNF killing. When cyclin D3 expression in HeLa cells was altered by sense or antisense cyclin D3 cDNA, there was a concomitant alteration in their susceptibility to TNF-induced apoptosis without any change in c-Myc levels. Overall, our results show that cyclin D3 sensitizes tumor cells to TNF-induced apoptosis and indicate that the expression of c-Myc and expression of cyclin D3 in HeLa and in HT-1080 fibrosarcoma cells are closely linked.
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Battiata, Andrew P., and John Casler. "Sclerosing Epithelioid Fibrosarcoma: A Case Report." Annals of Otology, Rhinology & Laryngology 114, no. 2 (February 2005): 87–89. http://dx.doi.org/10.1177/000348940511400201.
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Sclerosing epithelioid fibrosarcoma (SEF) is a rare variant of fibrosarcoma. It is a mesenchymal neoplasm composed of round to oval cells dispersed in a nestlike or cordlike distribution on a highly collagenous tissue background and is now recognized as a distinct clinical entity. In this report we discuss the presentation, diagnosis, and treatment of SEF. We focus on the unique histologic and immunohistochemical properties of this lesion. It is essential for both the surgeon and the pathologist to be aware of SEF and include it in the differential diagnosis for atypical head and neck neoplasms. Sclerosing epithelioid fibrosarcoma is a rare neoplasm that is now listed among the low-grade neoplasms that may occur in the head and neck. However, its behavior tends to be typical of more aggressive lesions. An awareness of this characteristic of SEF is essential for clinicians and pathologists alike.
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Popović, Dušica J., Dušan Lalošević, Kosta J. Popović, Ivan Čapo, Jovan K. Popović, and Dejan Miljković. "Effect of mebendazole on fibrosarcoma in hamsters." Tropical Journal of Pharmaceutical Research 16, no. 10 (November 15, 2017): 2445–51. http://dx.doi.org/10.4314/tjpr.v16i10.19.
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Purpose: To investigate the effect of mebendazole on an in vivo solid tumor model of fibrosarcoma in hamsters.Methods: 24 Syrian golden hamsters of both sexes with the approximate body weight of 100g were randomly distributed in 2 experimental and 2 control groups, with 6 animals in each group. BHK-21/C13 cells (2 x 106) in 1 mL Glasgow Minimum Essential Medium (GMEM) were injected subcutaneously into the back of each animal in 3 groups. The experimental groups were treated with mebendazole (460 mg/kg) via a gastric tube on a daily basis, immediately after tumor inoculation. In addition, one experimental group received deoxycholic acid 20 mg/kg once a day. After 2 weeks, when the tumors were approximately 1 - 2 cm in the control group, all the animals were sacrificed, and their blood collected for laboratory analysis. The tumors were excised, their weight and diameters measured, and the volumes calculated. The tumor samples were histopathologically assessed and the main organs toxicologically analyzed. Images were taken and processed by an imaging software, and Ki-67-positive cells in the tumor samples were quantified.Results: Mebendazole diminished tumor mitosis from 18.5 ± 3.02 to 13.5 ± 3.45 (p < 0.05), vasculature and tissue penetration, and increased necroses in tumor slices. Tumor volume and weight were insignificantly attenuated. Toxicity was not observed.Conclusion: Mebendazole might be an effective non-toxic agent in sarcoma therapy.Keywords: Mebendazole, Hamsters, BHK-21/C13 cells, Fibrosarcoma therapy, Tumor mitosis
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Lyu, Tae Seong, Yoojin Ahn, Young-Jun Im, Seong-Soo Kim, Ki-Heon Lee, Jinyoung Kim, Yujin Choi, et al. "The characterization of exosomes from fibrosarcoma cell and the useful usage of Dynamic Light Scattering (DLS) for their evaluation." PLOS ONE 16, no. 1 (January 26, 2021): e0231994. http://dx.doi.org/10.1371/journal.pone.0231994.
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Exosomes are a type of extracellular vesicles containing mRNA, miRNA, and proteins of origin cells, which can control the characteristics of other cells or surroundings. Despite increasing evidence on oncogenic properties of tumor-derived exosomes, fibrosarcoma-derived exosomes remain largely unrevealed. While the proper extraction and characterization of exosomes is critical in exosomes research, there are various limitations in techniques to measure the size and homogeneity of exosomes. Here, we analyzed exosomes from a fibrosarcoma cell line WEHI-164 compared with a breast cancer cell line MDA-MD-231 as a control. Results from dot blot and western blot analysis demonstrated that GM1 ganglioside, and TSG101, HSC70 and GAPDH proteins were contained in exosomes from the WEHI-164 fibrosarcoma cell line. The existence of tetraspanins such as CD81, CD63 and CD9 was confirmed in the exosomes by ExoView analysis. The results obtained from TEM showed their sphere-like shapes of around 50 to 70 nm in radius. Through DLS, we found out that the mean radius of the exosomes derived from WEHI-164 and MDA-MB-231 cell lines was 94.4 nm and 107.8 nm, respectively, with high homogeneity. When comparing the radius measured by TEM with the radius measured by DLS, it was revealed that the difference between the two methods was about 40 nm. This study has significance in characterizing the molecular properties of exosomes from a fibrosarcoma, which has not been researched much before, and in providing clear evidence that DLS can be used as an efficient, convenient and noninvasive technique to simply check the homogeneity and size of exosomes.
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Edelman, A. S., B. Xue, P. Sanchez, and G. J. Thorbecke. "Cross-reactive cellular and humoral immunity to carcinogen-induced chicken fibrosarcomas. I. Protective cross-immunity between transplantable tumor lines." Journal of Immunology 135, no. 3 (September 1, 1985): 2206–12. http://dx.doi.org/10.4049/jimmunol.135.3.2206.
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Abstract Dimethylbenz[a]anthracene (DMBA)-induced transplantable fibrosarcomas in B2 homozygous chickens (SC line) grow progressively in normal chickens, but are rejected by chickens immunized previously with irradiated tumor cells and Corynebacterium parvum. Tumor-immune chickens resist challenge by the immunizing tumor lines as well as by some, but not all, fibrosarcoma lines. The pattern of cross-reactivity between four DMBA-induced transplantable tumor lines was examined in detail. Ability to reject a tumor challenge correlated very well (p less than 0.001) with the presence of delayed-type hypersensitivity (DTH) to that tumor. Immunization with one of two of the DMBA-induced lines tested also caused rejection of transplantable tumors developed from methylcholanthrene-induced and benzo(a)pyrene-induced primary fibrosarcomas. Although immunization with tumor caused DTH to chicken embryo fibroblasts (CEF), immunization with CEF failed to cause protective immunity or DTH to tumors. Presence of protective immunity, where tested, also correlated with the ability of spleen cells from immune donors to inhibit tumor growth in Winn tests. Humoral immunity exhibited even greater cross-reactivity than did cellular immunity. Distinct patterns of cross-reactivity were nevertheless observed with respect to the serum antibodies as detected in ELISA. Two of these patterns were also observed in several sera from primary tumor-bearing chickens, both including reactivity with CEF. Such reactivity was absent from normal chicken sera.
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Clayer, M., S. Bouralexis, A. Evdokiou, S. Hay, GJ Atkins, and DM Findlay. "Enhanced Apoptosis of Soft Tissue Sarcoma Cells with Chemotherapy: A Potential New Approach Using Trail." Journal of Orthopaedic Surgery 9, no. 2 (December 2001): 19–22. http://dx.doi.org/10.1177/230949900100900205.
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Soft tissue sarcomas are less responsive to conventional chemotherapy when compared to bone sarcomas. We investigated the possibility of enhancing the efficacy of chemotherapy by utilising the recently identified cytokine, tumour necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) in combination with standard chemotherapeutic agents. Fresh human soft tissue sarcomas (rhabdomyosarcoma, fibrosarcoma, malignant fibrous histiocytoma) were obtained at biopsy and dispersed tumour cells were incubated in cell culture with standard cytotoxic agents, either as single agents or in combination with TRAIL. The chemotherapeutic agents were, at best, moderately effective, in terms of induction of cellular apoptosis, although the fibrosarcoma was completely unresponsive to all single agents. TRAIL alone had no effect on any sarcoma cell culture. In contrast, the addition of TRAIL and drug together produced a significant increase in sarcoma cell apoptosis, with TRAIL and doxorubicin the most effective combination.
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Talarico, D., M. M. Ittmann, R. Bronson, and C. Basilico. "A retrovirus carrying the K-fgf oncogene induces diffuse meningeal tumors and soft-tissue fibrosarcomas." Molecular and Cellular Biology 13, no. 4 (April 1993): 1998–2010. http://dx.doi.org/10.1128/mcb.13.4.1998-2010.1993.
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The K-fgf/hst oncogene encodes a growth factor of the fibroblast growth factor (FGF) family and transforms cells through an autocrine mechanism which requires extracellular activation of its receptor(s). To identify the cell and tissue targets of K-fgf oncogenic potential in vivo, we constructed a recombinant retrovirus carrying the human K-fgf cDNA and injected it, together with helper Moloney murine leukemia virus, into immunocompetent as well as nude mice. The original construct was highly transforming in tissue culture but produced no detectable pathologies in vivo with the exception of a single fibrosarcoma which arose after a long latency. The virus produced by this tumor appears to have undergone a complex series of recombination events involving the helper Moloney murine leukemia virus. It encodes an Env/K-FGF fusion protein whose expression is under the control of a hybrid long terminal repeat. This virus (designated MFS, for meningeal fibrosarcoma) induces tumors in mice with high frequency and short latency. These neoplasms consist of aggressive fibrosarcomas of soft tissue as well as diffuse meningeal tumors originating from the dura mater that surround the whole central nervous system and cause severe hydrocephalus. The Env/K-FGF fusion protein expressed by the MFS virus has retained all of the biological properties of native K-FGF, including secretion, mitogenic activity, heparin binding, and neutralization by anti-K-FGF antibodies. These and other results indicate that the tumors induced by the MFS virus result from the oncogenic potential of K-FGF.
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Marrero, Ana Dácil, Ana R. Quesada, Beatriz Martínez-Poveda, Miguel Ángel Medina, and Casimiro Cárdenas. "A Proteomic Study of the Bioactivity of Annona muricata Leaf Extracts in HT-1080 Fibrosarcoma Cells." International Journal of Molecular Sciences 24, no. 15 (July 27, 2023): 12021. http://dx.doi.org/10.3390/ijms241512021.
Full textAbstract:
Graviola (Annona muricata) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic approach to detect altered patterns in proteins on both conditioned media and extracts of HT-1080 fibrosarcoma cells under treatment conditions, revealing new potential bioactivities of Annona muricata extracts. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from Annona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated in vitro. Additional experimental data reveal that DMSO extracts protect HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells from ferroptosis. Data from our proteomic study are available via ProteomeXchange with identifier PXD042354.
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Servato, João Paulo Silva, Paulo Rogério de Faria, Cássio Vinhadelli Ribeiro, Sergio Vitorino Cardoso, Paulo Rogério de Faria, Fernando Luiz Dias, Ana Lúcia Amaral Eisenberg, and Adriano Mota Loyola. "Ameloblastic Fibrosarcoma: A Case Report and Literature Review." Brazilian Dental Journal 28, no. 2 (April 2017): 262–72. http://dx.doi.org/10.1590/0103-6440201701050.
Full textAbstract:
Here is described a case of ameloblastic fibrosarcoma (AFS) affecting the posterior mandible of a woman who was treated surgically and recovered without signs of recurrence or metastasis after 12 years of follow-up. Tumor sections were immunostained for cell cycle, epithelial and mesenchymal markers. Immunohistochemical analysis evidenced high Ki-67 positivity in stromal cells (mean of 20.9 cells/High power field). Epithelial cells displayed strong positivity for p53, p63 and cytokeratin 19. In addition to the case report, a systematic review of current knowledge is presented on the AFS’s clinical-demographic features and prognostic factors. Based on the review, 88/99 cases were diagnosed as AFS, 9/99 as ameloblastic fibro-odontosarcoma and 2/99 as ameloblastic fibrodentinosarcoma. All these lesions displayed very similar clinical-demographic and prognostic features. Moreover, the review provided evidence that first treatment, regional metastasis, distant metastasis and local recurrence were significant prognostic values for malignant odontogenic mesenchymal lesions. Based on the findings, segregation among ameloblastic fibrosarcoma, ameloblastic fibrodentinosarcoma and ameloblastic fibro-odontosarcoma seems illogical, considering all these lesions have similar predilections and outcomes.
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Iguchi-Manaka, Akiko, Hirayasu Kai, Yumi Yamashita, Kai Shibata, Satoko Tahara-Hanaoka, Shin-ichiro Honda, Teruhito Yasui, Hitoshi Kikutani, Kazuko Shibuya, and Akira Shibuya. "Accelerated tumor growth in mice deficient in DNAM-1 receptor." Journal of Experimental Medicine 205, no. 13 (November 24, 2008): 2959–64. http://dx.doi.org/10.1084/jem.20081611.
Full textAbstract:
Since the identification of ligands for human and mouse DNAM-1, emerging evidence has suggested that DNAM-1 plays an important role in the T cell– and natural killer (NK) cell–mediated recognition and lysis of tumor cells. However, it remains undetermined whether DNAM-1 is involved in tumor immune surveillance in vivo. We addressed this question by using DNAM-1–deficient mice. DNAM-1–deficient cytotoxic T lymphocyte (CTL) and NK cells showed significantly less cytotoxic activity against DNAM-1 ligand-expressing tumors in vitro than wild-type (WT) cells. The methylcholanthrene (MCA)-induced fibrosarcoma cell line Meth A expressed the DNAM-1 ligand CD155, and DNAM-1–deficient mice showed increased tumor development and mortality after transplantation of Meth A cells. Moreover, the DNAM-1–deficient mice developed significantly more DNAM-1 ligand-expressing fibrosarcoma and papilloma cells in response to the chemical carcinogens MCA and 7,12-dimethylbenz[a]anthracene (DMBA), respectively, than did WT mice. These results indicate that DNAM-1 plays an important role in immune surveillance of tumor development.
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Lazim, A., and R. Kuklani. "Sclerosing Epithelioid Fibrosarcoma; A Case report and review of literature." American Journal of Clinical Pathology 156, Supplement_1 (October 1, 2021): S89. http://dx.doi.org/10.1093/ajcp/aqab191.189.
Full textAbstract:
Abstract Introduction/Objective Sclerosing Epithelioid Fibrosarcoma (SEF) is an unusual, rare clinically aggressive form of soft tissue sarcoma. It is characterized by a slow evolution, with local recurrences and late metastases that are mainly pulmonary and pleural in about 80% of cases. SEF has distinctive morphology and occurs most commonly in deep soft tissue of adult extremities. Lesions involving the head and neck region are uncommon and rare intraosseously in oral cavity. Methods/Case Report Herein we report a case of a 52-year-old male who presented with a symptomatic radiolucent lesion at apex #18, with clinical impression of periapical granuloma. The patient did present with pain associated with lower left quadrant #18 area, for several days to pressure and hot temperature. A periapical radiograph revealed a large ill-defined radiolucent area at apex of resorbing roots #18. The patient’s medical history was significant with history of cancer diagnosis of sclerosing epithelioid fibrosarcoma of skull treated by chemotherapy and radiation. Microscopic examination of the specimen revealed a multi-fragmented specimen consisting of numerous fragments and islands of highly cellular, basophilic bone and osteoid surrounded by loose fibrous stroma which contains large lobules and islands of round to oval cells, with distinct cell borders and faintly granular eosinophilic cytoplasm. Separate islands of tumor cells surround large islands of necrosis with the background stroma appearing as hyalinized and eosinophilic with the basaloid cells demonstrating smudge and crush artifact. Peripherally, spindled cells are noted and faint areas of eosinophilic osteoid within the eosinophilic background stroma. Lesional cells are MUC4 strong, diffusely positive and strongly positive for INI-1. CD43, CD20, PAX-5, CD3, Desmin, CD34, S100, CD99, AE1/3 and SMA are interpreted to be negative. A diagnosis of metastatic sclerosing epithelioid fibrosarcoma was made, with correlation with the primary lesion was recommended. Results (if a Case Study enter NA) N/A Conclusion The clinical and histological findings of our case correlate with the diagnostic criteria of sclerosing epithelioid fibrosarcoma. Despite its microscopic features can create diagnostic difficulties, in that SEF can resemble a variety of benign and pseudosarcomatous as well as malignant lesions, our case diagnosis was confirmed by morphology and MUC4 diffuse strong reactivity and negativity for other markers.
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Talarico, D., M. M. Ittmann, R. Bronson, and C. Basilico. "A retrovirus carrying the K-fgf oncogene induces diffuse meningeal tumors and soft-tissue fibrosarcomas." Molecular and Cellular Biology 13, no. 4 (April 1993): 1998–2010. http://dx.doi.org/10.1128/mcb.13.4.1998.
Full textAbstract:
The K-fgf/hst oncogene encodes a growth factor of the fibroblast growth factor (FGF) family and transforms cells through an autocrine mechanism which requires extracellular activation of its receptor(s). To identify the cell and tissue targets of K-fgf oncogenic potential in vivo, we constructed a recombinant retrovirus carrying the human K-fgf cDNA and injected it, together with helper Moloney murine leukemia virus, into immunocompetent as well as nude mice. The original construct was highly transforming in tissue culture but produced no detectable pathologies in vivo with the exception of a single fibrosarcoma which arose after a long latency. The virus produced by this tumor appears to have undergone a complex series of recombination events involving the helper Moloney murine leukemia virus. It encodes an Env/K-FGF fusion protein whose expression is under the control of a hybrid long terminal repeat. This virus (designated MFS, for meningeal fibrosarcoma) induces tumors in mice with high frequency and short latency. These neoplasms consist of aggressive fibrosarcomas of soft tissue as well as diffuse meningeal tumors originating from the dura mater that surround the whole central nervous system and cause severe hydrocephalus. The Env/K-FGF fusion protein expressed by the MFS virus has retained all of the biological properties of native K-FGF, including secretion, mitogenic activity, heparin binding, and neutralization by anti-K-FGF antibodies. These and other results indicate that the tumors induced by the MFS virus result from the oncogenic potential of K-FGF.