Journal articles on the topic 'Fibroblastes gingivaux'
To see the other types of publications on this topic, follow the link: Fibroblastes gingivaux.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Fibroblastes gingivaux.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Lauritano, Dorina, Alberta Lucchese, Dario Di Stasio, Fedora Della Vella, Francesca Cura, Annalisa Palmieri, and Francesco Carinci. "Molecular Aspects of Drug-Induced Gingival Overgrowth: An In Vitro Study on Amlodipine and Gingival Fibroblasts." International Journal of Molecular Sciences 20, no. 8 (April 25, 2019): 2047. http://dx.doi.org/10.3390/ijms20082047.
Abstract:
Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the fibrotic response, we investigated its effects on treated gingival fibroblast gene expression as compared with untreated cells. Materials and Methods: Fibroblasts from ATCC® Cell Lines were incubated with amlodipine. The gene expression levels of 12 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway was investigated in treated fibroblasts cell culture, as compared with untreated cells, by real time PCR. Results: Most of the significant genes were up-regulated. (CTNND2, COL4A1, ITGA2, ITGA7, MMP10, MMP11, MMP12, MMP26) except for COL7A1, LAMB1, MMP8, and MMP16, which were down-regulated. Conclusion: These results seem to demonstrate that amlodipine has an effect on the extracellular matrix of gingival fibroblast. In the future, it would be interesting to understand the possible effect of the drug on fibroblasts of patients with amlodipine-induced gingival hyperplasia.
2
Liu, Kaining, Bing Han, Jianxia Hou, Jianyun Zhang, Jing Su, and Huanxin Meng. "Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo." PeerJ 9 (January 4, 2021): e10279. http://dx.doi.org/10.7717/peerj.10279.
Abstract:
Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Results Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Conclusions Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.
3
Smith, Q. T., and J. E. Hinrichs. "Phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not Alter the Effects of Bacterial and Amplified Plaque Extracts on Cultures of Fibroblasts from Normal and Overgrown Gingivae." Journal of Dental Research 66, no. 8 (August 1987): 1393–98. http://dx.doi.org/10.1177/00220345870660082201.
Abstract:
Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and "amplified" plaque extracts, and (c) microbial and "amplified" plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.
4
Francetti, Luca, Claudia Dellavia, Stefano Corbella, Nicolò Cavalli, Claudia Moscheni, Elena Canciani, and Nicoletta Gagliano. "Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses." Case Reports in Dentistry 2019 (May 22, 2019): 1–10. http://dx.doi.org/10.1155/2019/3231759.
Abstract:
Gingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronic traumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues in genetically predisposed subjects. Since an imbalance in collagen turnover plays a key role in the development of gingival overgrowth leading to an accumulation of collagen in gingival connective tissue, in this study we described the histological and molecular features of three oral overgrowths obtained from a 34-year-old woman previously operated for FGG in order to evaluate a possible relationship between exostoses and overgrown tissue. Healthy and overgrown gingiva were analyzed by histological methods, and the expression of genes and proteins involved in collagen synthesis, maturation, and degradation was assessed in cultured fibroblasts obtained from gingival fragments at the molecular level. Our results show that general morphology and collagen content were similar in healthy and overgrown gingivae. However, fibroblasts obtained from the overgrown gingiva revealed an anabolic phenotype characterized by an increased collagen turnover and maturation. These findings indicate that an exostosis could act as a mechanical stimulus stretching the overlying connective tissue and triggering an anabolic phenotype of gingival fibroblasts and suggest to use minimally invasive surgical techniques to avoid traumatizing the periosteal tissues for the eradication of the exostosis with minimal relapses.
5
Danastri, Arifia Anindita, Suryono Suryono, and Kwartarini Murdiastuti. "THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION." ODONTO : Dental Journal 8, no. 2 (December 22, 2021): 25. http://dx.doi.org/10.30659/odj.8.2.25-31.
Abstract:
ABSTRACTBackground: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.
6
Lauritano, Dorina, Marcella Martinelli, Alessandro Baj, Giada Beltramini, Valentina Candotto, Francesco Ruggiero, and Annalisa Palmieri. "Drug-induced gingival hyperplasia: An in vitro study using amlodipine and human gingival fibroblasts." International Journal of Immunopathology and Pharmacology 33 (January 2019): 205873841982774. http://dx.doi.org/10.1177/2058738419827746.
Abstract:
Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 ( CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.
7
Walters, J. D., R. J. Nakkula, and P. Maney. "Modulation of Gingival Fibroblast Minocycline Accumulation by Biological Mediators." Journal of Dental Research 84, no. 4 (April 2005): 320–23. http://dx.doi.org/10.1177/154405910508400405.
Abstract:
Gingival fibroblasts actively accumulate tetracyclines, thereby enhancing their redistribution from blood to gingiva. Since growth factors and pro-inflammatory cytokines regulate many fibroblast activities, they could potentially enhance fibroblast minocycline accumulation. To test this hypothesis, we treated gingival fibroblast monolayers for 1 or 6 hours with platelet-derived growth factor-BB (PDGF), fibroblast growth factor-2 (FGF), transforming growth factor-β1 (TGF), or tumor necrosis factor-α (TNF). Minocycline uptake was assayed at 37° by a fluorescence method. All 4 factors significantly enhanced minocycline uptake (P ≤ 0.008, ANOVA), primarily by increasing the affinity of transport. Treatment for 6 hours with 10 ng/mL FGF, PDGF, TGF, or TNF enhanced fibroblast minocycline uptake by 19% to 25%. Phorbol myristate acetate enhanced fibroblast minocycline uptake by 28%, suggesting that protein kinase C plays a role in up-regulating transport. These effects on transport provide a mechanism by which systemic tetracyclines could be preferentially distributed to gingival wound or inflammatory sites.
8
Ruggeri, A., L. Montebugnoli, A. Matteucci, N. Zini, L. Solimando, D. Servidio, P. Suppa, M. Cadenaro, L. Cocco, and L. Breschi. "Cyclosporin A Specifically Affects Nuclear PLCβ1 in Immunodepressed Heart Transplant Patients with Gingival Overgrowth." Journal of Dental Research 84, no. 8 (August 2005): 747–51. http://dx.doi.org/10.1177/154405910508400812.
Abstract:
One of the most commonly observed adverse effects of cyclosporin A (CsA) is the development of gingival overgrowth (GO). Fibroblasts are involved in GO, but the question why only a percentage of patients undergoing CsA treatment shows this side-effect remains unanswered. In a previous study, CsA has been demonstrated to induce over-expression of phospholipase C (PLC) β1 in fibroblasts of patients with clinical GO, in cells from both enlarged and clinically healthy gingival sites. In this work, we assessed the expression of PLCβ isoforms to investigate whether the exaggerated fibroblast response to CsA related to increased PLCβ1 expression could also be detected in CsA-treated patients without clinical signs of GO. Our results support the hypothesis of a multi-factorial origin of gingival overgrowth, including specific changes within the gingival tissues orchestrating fibroblastic hyper-responsiveness as a consequence of a long-term in vivo exposure to cyclosporin A.
9
Damanaki, Anna, Marjan Nokhbehsaim, Sigrun Eick, Werner Götz, Jochen Winter, Gerhard Wahl, Andreas Jäger, Søren Jepsen, and James Deschner. "Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies." Mediators of Inflammation 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/912821.
Abstract:
Adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), are molecules, which are produced in adipose tissue. Recent studies suggest that NAMPT might also be produced in the tooth-supporting tissues, that is, periodontium, which also includes the gingiva. The aim of this study was to examine if and under what conditions NAMPT is produced in gingival fibroblasts and biopsies from healthy and inflamed gingiva. Gingival fibroblasts produced constitutively NAMPT, and this synthesis was significantly increased by interleukin-1βand the oral bacteriaP. gingivalisandF. nucleatum. Inhibition of the MEK1/2 and NFκB pathways abrogated the stimulatory effects ofF. nucleatumon NAMPT. Furthermore, the expression and protein levels of NAMPT were significantly enhanced in gingival biopsies from patients with periodontitis, a chronic inflammatory infectious disease of the periodontium, as compared to gingiva from periodontally healthy individuals. In summary, the present study provides original evidence that gingival fibroblasts produce NAMPT and that this synthesis is increased under inflammatory and infectious conditions. Local synthesis of NAMPT in the inflamed gingiva may contribute to the enhanced gingival and serum levels of NAMPT, as observed in periodontitis patients. Moreover, local production of NAMPT by gingival fibroblasts may represent a possible mechanism whereby periodontitis may impact on systemic diseases.
10
Kujundzic, Bojan, Zlatibor Andjelkovic, Radmila Maric, Ruzica Lukic, Veljko Maric, Helena Maric, Miroslav Obrenovic, and Sinisa Kojic. "Periodontal Disease: Correlation with Histological and Immunological Parameters." Experimental and Applied Biomedical Research (EABR) 24, no. 1 (March 1, 2023): 57–62. http://dx.doi.org/10.2478/sjecr-2020-0024.
Abstract:
Abstract Periodontal disease is inflammatory pathological conditions in the gingiva and dental support structures that usually results in extracellular matrix and connective tissue destruction. During periodontitis, inflammatory cells facilitate collagen and connective tissue loss, affects the number and activity of fibroblasts and its production of local collagen networks. Aim of this study was to evaluate collagen density and accumulation of collagen producing fibroblast and macrophages in affected tissue of periodontal disease. Histological and immunohistochemical analyzes were performed on paraffin embedded tissue sections of gingival biopsies, obtained from 30 patients with diagnosis of periodontal disease and 10 healthy donors. Tissue sections of gingival of patients with periodontal disease had significantly decreased collagen volume density and visible fragmentation and lysis of the collagen fibers, decreased number of fibroblasts, accompanied with increased accumulation of macrophages. Presented data implicate that macrophages accumulation may be the cause of enzyme mediated collagen destruction
11
Lee, E. J., S. I. Jang, D. Pallos, J. Kather, and T. C. Hart. "Characterization of Fibroblasts with Son of Sevenless-1 Mutation." Journal of Dental Research 85, no. 11 (November 2006): 1050–55. http://dx.doi.org/10.1177/154405910608501115.
Abstract:
Although non-syndromic hereditary gingival fibromatosis (HGF) is genetically heterogeneous, etiologic mutations have been identified only in the Son of Sevenless-1 gene ( SOS1). To test evidence of increased cell proliferation, we studied histological, morphological, and proliferation characteristics in monolayer and three-dimensional cultures of fibroblasts with the SOS1 g.126,142–126,143insC mutation. Histological assessment of HGF gingiva indicated increased numbers of fibroblasts (30%) and increased collagen (10%). Cell proliferation studies demonstrated increased growth rates and 5-bromo-2-deoxyuridine incorporation for HGF fibroblasts. Flow cytometry showed greater proportions of HGF fibroblasts in the G2/M phase. Attachment of HGF fibroblasts to different extracellular matrix surfaces demonstrated increased formation of protrusions with lamellipodia. HGF fibroblasts in three-dimensional culture showed greater cell proliferation, higher cell density, and alteration of surrounding collagen matrix. These findings revealed that increased fibroblast numbers and collagen matrix changes are associated with mutation of the SOS1 gene in vitro and in vivo.
12
McCrudden, Maelíosa, Katherine O’Donnell, Chris Irwin, and Fionnuala Lundy. "Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling?" Vaccines 6, no. 3 (July 23, 2018): 44. http://dx.doi.org/10.3390/vaccines6030044.
Abstract:
Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues.
13
Rodrigues, Annelissa Zorzeto, Paulo Tambasco de Oliveira, Arthur Belém Novaes Jr., Luciana Prado Maia, Sérgio Luís Scombatti de Souza, and Daniela Bazan Palioto. "Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix." Brazilian Dental Journal 21, no. 3 (2010): 179–89. http://dx.doi.org/10.1590/s0103-64402010000300001.
Abstract:
The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.
14
Gund, Madline P., Jusef Naim, Antje Lehmann, Matthias Hannig, Constanze Linsenmann, Axel Schindler, and Stefan Rupf. "Effects of Cold Atmospheric Plasma Pre-Treatment of Titanium on the Biological Activity of Primary Human Gingival Fibroblasts." Biomedicines 11, no. 4 (April 16, 2023): 1185. http://dx.doi.org/10.3390/biomedicines11041185.
Abstract:
Cold atmospheric plasma treatment (CAP) enables the contactless modification of titanium. This study aimed to investigate the attachment of primary human gingival fibroblasts on titanium. Machined and microstructured titanium discs were exposed to cold atmospheric plasma, followed by the application of primary human gingival fibroblasts onto the disc. The fibroblast cultures were analyzed by fluorescence, scanning electron microscopy and cell-biological tests. The treated titanium displayed a more homogeneous and denser fibroblast coverage, while its biological behavior was not altered. This study demonstrated for the first time the beneficial effect of CAP treatment on the initial attachment of primary human gingival fibroblasts on titanium. The results support the application of CAP in the context of pre-implantation conditioning, as well as of peri-implant disease treatment.
15
Furukawa, M., Jr K-Kaneyama, M. Yamada, A. Senda, A. Manabe, and A. Miyazaki. "Cytotoxic Effects of Hydrogen Peroxide on Human Gingival Fibroblasts In Vitro." Operative Dentistry 40, no. 4 (June 1, 2015): 430–39. http://dx.doi.org/10.2341/14-059-l.
Abstract:
SUMMARY In-office bleaching is a popular treatment in modern esthetic dentistry. However, bleaching agents sometimes accidentally adhere to the gingiva and peripheral tissues, even when applied by well-trained dentists. This can lead to transient pain and whitish changes in the gingiva. Although these symptoms disappear within several hours, the effects of bleaching agents on gingiva have not been well described in the literature. The present study aimed to elucidate the cytotoxic effects of a bleaching agent on cultured human gingival fibroblasts (HGFs). We performed a comprehensive analysis of the toxic effects of in-office bleaching agents on gingiva using cultured HGFs and DNA microarray. Survival rates of HGFs decreased with increases in the concentration of hydrogen peroxide, which became significant at concentrations of 1.5 × 10−3% or higher at every time point. Concentrations lower than 1.5 × 10−3% did not affect survival rates of HGFs. Cytotoxicity of hydrogen peroxide was significantly weakened by the addition of vitamin E. Stimulation by in-office bleaching agents triggered the proinflammatory cytokine tumor necrosis factor (TNF)–α cascade in gingival fibroblasts. As the TNF-α cascade can be inhibited by vitamin E additives, treatment with vitamin E may protect gingival fibroblasts against the toxic effects of an in-office bleaching agent. The present results suggest that local administration of vitamin E to gingiva before in-office bleaching may be useful for preventing gingival irritation due to accidental adhesion of a bleaching agent.
16
Guo, Fen, David E. Carter, and Andrew Leask. "miR-218 regulates focal adhesion kinase–dependent TGFβ signaling in fibroblasts." Molecular Biology of the Cell 25, no. 7 (April 2014): 1151–58. http://dx.doi.org/10.1091/mbc.e13-08-0451.
Abstract:
Scarring, which occurs in essentially all adult tissue, is characterized by the excessive production and remodeling of extracellular matrix by α-smooth muscle actin (SMA)–expressing myofibroblasts located within connective tissue. Excessive scarring can cause organ failure and death. Oral gingivae do not scar. Compared to dermal fibroblasts, gingival fibroblasts are less responsive to transforming growth factor β (TGFβ) due to the reduced expression, due to the reduced expression and activity of focal adhesion kinase (FAK) by this cell type. Here we show that, compared with dermal fibroblasts, gingival fibroblasts show reduced expression of miR-218. Introduction of pre–miR-218 into gingival fibroblasts elevates FAK expression and, via a FAK/src-dependent mechanism, results in the ability of TGFβ to induce α-SMA. The deubiquitinase cezanne is a direct target of miR-218 and has increased expression in gingival fibroblasts compared with dermal fibroblasts. Knockdown of cezanne in gingival fibroblasts increases FAK expression and causes TGFβ to induce α-smooth muscle actin (α-SMA). These results suggest that miR-218 regulates the ability of TGFβ to induce myofibroblast differentiation in fibroblasts via cezanne/FAK.
17
Widya, Widya Rizky Romadhona. "Viabilty Test of Fish Scales Collagen Gourami (Oshpronemus Gouramy) on Human Gingival Fibroblasts Cells." Journal of Stem Cell Research and Tissue Engineering 6, no. 1 (September 12, 2022): 32–38. http://dx.doi.org/10.20473/jscrte.v6i1.37516.
Abstract:
Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding the teeth, including Human Gingival Fibroblasts (HGF) which is one of the major components of tissue formation in periodonsium. HGF regeneration with the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering using regenerative materials from cow or pig as the therapies, but these materials have some flaws so this research to find alternative materials regenerative tissue engineering scaffold collagen type 1 derived from fresh water fish scales, one of which are gourami fish scales. This research was conducted to test the viability of fish scales collagen gouramy against Human Gingival Fibroblasts for 24 hours. This study aimed to determine the concentration of fish scale collagen gourami which can maintain the viability of human gingival fibroblast cells for 24 hours. HGF is taken from healthy gingiva and planted in 96 well plates. Fish scales collagen gouramy with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells. The viability of HGF were increased after the addition of fish scales collagen gourami on concentration 0.32 mg/ml until 0.01 mg/ml. The highest viability of the cells was shown after the addition of 0.01 mg/ml. Fish scales collagen gouramy has the potential in tissue engeneering and the concentration of 0.01 mg/ml shows the highest viability of HGF.
18
Gagliano, N., F. Costa, G. M. Tartaglia, L. Pettinari, F. Grizzi, C. Sforza, N. Portinaro, M. Gioia, and G. Annoni. "Effects of Aging and Cyclosporin A on Collagen Turnover in Human Gingiva." Open Dentistry Journal 3, no. 1 (November 5, 2009): 219–26. http://dx.doi.org/10.2174/1874210600903010219.
Abstract:
Background: We aimed at characterizing the aging gingiva analyzing: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects. Methods: Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration. Results: Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts. Conclusions: Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications.
19
Hu, Jiangqi, Yue Qie, Yu Luo, and Qingsong Jiang. "Effect of Porous Zirconia Coating on Human Gingival Fibroblasts and Its Mechanism." Journal of Biomedical Nanotechnology 18, no. 4 (April 1, 2022): 1164–71. http://dx.doi.org/10.1166/jbn.2022.3317.
Abstract:
Gingival fibroblasts play an important role in the constitution of soft tissue attachment. This study aims to investigate whether porous zirconia coating has a positive effect on promoting human gingival fibroblast attachment. The porous zirconia coating was loaded on zirconia surface by the dip coating method, surface morphology and composition were confirmed by scanning electron microscope and energy dispersive spectrometer; Tested the tensile bond strength by universal testing machine; Tested the surface roughness by roughness analyzer; Human gingival fibroblast proliferation, integrin β1 and F-actin immunofluorescence staining explored the influence of porous zirconia on the adhesion and proliferation of human gingival fibroblast. Zirconia0.2 group showed spherical zirconia particles with diameters of 3–8 μm are distributed on the surface; The bonding strength of zirconia particle coating group reached 16.1±0.1 MPa, and the surface roughness was 0.715±0.091 μm; In comparison with control group (P < 0.01), the percentage of human gingival fibroblasts adhering to zirconia was markedly higher. In zirconia group, integrin-β1 and F-actin fluoresced more obvious than in control group. Porous zirconia coating can form a porous structure on the surface and the porous structure can promote the attachment and proliferation of human gingival fibroblast, it will be more beneficial for soft tissue early sealing.
20
Havemose-Poulsen, Anne, and Palle Holmstrup. "Factors Affecting IL-1-Mediated Collagen Metabolism By Fibroblasts and the Pathogenesis of Periodontal Disease: A Review of the Literature." Critical Reviews in Oral Biology & Medicine 8, no. 2 (April 1997): 217–36. http://dx.doi.org/10.1177/10454411970080020801.
Abstract:
Fibroblasts have been studied extensively for their contribution to connective tissue destruction in diseases where the metabolism of extracellular matrix components plays an essential part in their pathogenesis. A considerable dissolution, especially of collagen fibrils, is a well-known characteristic of the periodontal ligament and the gingival connective tissue in microbial-induced periodontal disease. Fibroblasts, responsible for the assembly of the extracellular matrix, are capable of responding directly to oral microbial challenges or indirectly, following activation of the host immune response, and can alter the composition of connective tissue in several ways: synthesis of inflammatory mediators, their receptors and antagonists; fibroblast proliferation; collagen synthesis; phagocytosis of collagen fibrils; and synthesis of proteolytic enzymes, including matrix metalloproteinases and their corresponding inhibitors. The contributions of these cellular fibroblastic properties to the pathogenesis of periodontal disease are reviewed in the context of the cytokine, interleukin-1, as the inflammatory regulator.
21
Speranskaya, Ekaterina M., Al'bina F. Saleeva, Lyubov R. Mukhamedzhanova, and Natal'ya N. Golubtsova. "THE PROLIFERATIVE ACTIVITY OF FIBROBLASTS OF GINGIVA IN ADULTS AT CHRONIC PERIODONTITIS." Morphological newsletter 31, no. 2 (March 20, 2023): 69–76. http://dx.doi.org/10.20340/mv-mn.2023.31(2).745.
Abstract:
Periodontitis is a disease that progresses with age and contributes to the biological aging of the dentition. One of the reasons for this phenomenon is the aging of human gum fibroblasts. Low-intensity laser therapy stimulates the release of growth factors from fibroblasts and their division. The aim of the study was to determine the number, proliferative activity and apoptosis of gingival fibroblasts in people without signs of periodontal inflammation, with chronic periodontitis and after laser therapy. We used biopsy material obtained for periodontal, surgical, orthopedic and orthodontic indications under infiltration anesthesia in two age groups of patients aged 20–40 and 41–60 years. Cell markers Ki-67 and p53 were detected by immunohistochemical methods, the total number of gingival fibroblasts was estimated by staining with hematoxylin and eosin. The results of the conducted studies showed that the number of fibroblasts in the connective tissue lamina of the gums has age-related features. In young people from 20 to 40 years, the number of fibroblasts in gum preparations is 1,12 times higher than in people aged 41–60 years. Among the reasons for the decrease in the number of gingival fibroblasts is a decrease in their proliferative activity and activation of the proapoptotic p53 protein with age. Chronic periodontal inflammation leads to a significant increase in the number of fibroblasts, regardless of the age of the subjects. Fibroblast proliferation and p53 protein expression were more sensitive to microbial inflammation in patients aged 41 to 60 years compared to younger patients. Three-fold laser exposure had a differentiated modulating effect on the state of the gingival fibroblast population, depending on the age of the patients, the number of gingival fibroblasts was restored to the levels characteristic of healthy people, and this was observed only in the group of young patients. Thus, the number of fibroblasts in the human gingival connective tissue lamina changes with age, under conditions of inflammation and under the influence of a diode laser, which must be taken into account in dental practice.
22
Suzuki, A. M. M., A. Yoshimura, Y. Ozaki, T. Kaneko, and Y. Hara. "Cyclosporin A and Phenytoin Modulate Inflammatory Responses." Journal of Dental Research 88, no. 12 (November 6, 2009): 1131–36. http://dx.doi.org/10.1177/0022034509350566.
Abstract:
Gingival overgrowth is a common side-effect of administration of the immunosuppressant cyclosporin A and the anti-epileptic drug phenytoin. While cyclosporin-induced gingival overgrowth is often accompanied by gingival inflammation, phenytoin-induced gingival overgrowth usually forms fibrotic lesions. To determine whether these drugs alter the inflammatory responses of gingival fibroblasts, we investigated the effects of cyclosporin and phenytoin on Toll-like receptor (TLR)-mediated responses to microbial components. In Chinese hamster ovary reporter cell lines, cyclosporin alone triggered signaling, whereas phenytoin down-regulated signaling induced by the TLR2 or TLR4 ligand. In human gingival fibroblasts, cyclosporin alone did not induce evident inflammatory responses, but augmented the expression of CD54 and the production of interleukin (IL)-6 and IL-8 induced by TLR ligands, whereas phenytoin attenuated those responses. Cyclosporin also augmented CD54 expression in gingiva of mice injected with lipopolysaccharide. These results indicated that cyclosporin positively and phenytoin negatively modulated inflammatory responses of human gingival fibroblasts.
23
Irwin, C. R., M. Picardo, I. Ellis, P. Sloan, A. Grey, M. McGurk, and S. L. Schor. "Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing." Journal of Cell Science 107, no. 5 (May 1, 1994): 1333–46. http://dx.doi.org/10.1242/jcs.107.5.1333.
Abstract:
We have previously reported that fetal and adult skin fibroblasts display distinctive migratory phenotypes on 3-D collagen substrata and that these behavioural characteristics may be quantified by a function defined as the cell density migration index (CDMI). Subsequent work indicated that this difference in migratory phenotype was due to the production by fetal fibroblasts of a migration stimulating factor (MSF) that is not produced by normal adult skin fibroblasts. We now present data indicating that: (a) unselected fibroblasts obtained from 14/14 (100%) of adult gingival explants expressed fetal-like CDMI values compared to only 1/10 (10%) of similarly explanted paired skin cells; (b) 12/12 (100%) of these gingival fibroblast lines also produced detectable quantities of MSF compared to 0/9 (0%) of the tested skin cells; (c) by microdissection studies, gingival fibroblasts obtained from different anatomical microdomains consisted of behaviourally distinct subpopulations, with cells derived from the papillary tips (PAP fibroblasts) displaying fetal-like CDMI values and persistent MSF production, whilst cells obtained from the deeper reticular tissue (RET fibroblasts) were adult-like with respect to these two criteria; (d) PAP fibroblasts were also smaller and achieved higher saturation cell densities compared to paired RET cells; (e) PAP fibroblasts passaged in vitro underwent a fetal-to-adult phenotypic transition characterized by the adoption of various RET cell characteristics, including the acquisition of CDMI values falling within the adult range and cessation in MSF production; and (f) early passage PAP fibroblasts incubated in the presence of an affinity-purified anti-MSF rabbit polyclonal antibody were induced to alter their migratory phenotype and exhibited CDMI values falling within the adult range. Statistical analysis indicated a highly significant correlation between the expression of a fetal-like CDMI and production of MSF (P < 0.00001, using the Fisher exact contingency test). Taken together, these observations suggest that the production of MSF by PAP fibroblasts is responsible for their characteristically fetal-like migratory behaviour. The existence of such inter- and intra-site phenotypic heterogeneity in populations of skin and gingival fibroblasts is discussed in the context of fibroblast lineage relationships and the possible contribution of persistently fetal-like fibroblast subpopulations to connective tissue function in wound healing.
24
Meilawaty, Zahara, Amandia Dewi Permana Shita, Paramudibta Lungit Kuncaraningtyas, Agustin Wulan Suci Dharmayanti, and Zahreni Hamzah. "Potensi ekstrak daun singkong (Manihot esculenta Crantz) terhadap ekspresi MMP-8 fibroblas gingiva pada model tikus dengan disfungsi ovarium dan periodontitisPotential of cassava (Manihot esculenta Crantz) leaf extract on the MMP-8 expression of gingival fibroblast in rats model with ovarian dysfunction and periodontitis." Jurnal Kedokteran Gigi Universitas Padjadjaran 32, no. 2 (August 31, 2020): 105. http://dx.doi.org/10.24198/jkg.v32i2.27466.
Abstract:
Pendahuluan: Disfungsi ovarium merupakan kondisi yang menimbulkan defisiensi hormon estrogen dan progesteron. Penurunan hormonal ini menyebabkan peningkatan produksi tumor necrosis factor (TNF) yang dapat memicu peningkatan produksi matriks metalloproteinase-8 (MMP-8). Periodontitis yang disebabkan oleh bakteri gram negatif akan memicu makrofag melepaskan TNF-α yang berkontribusi dalam pembentukan MMP-8. MMP-8 ini berperan dalam degradasi kolagen jaringan ikat gingiva. Meningkatnya MMP-8 dapat menyebabkan terjadinya periodontitis. Gejala periodontitis karena disfungsi ovarium dan induksi bakteri ini dapat diperlambat dengan bahan alam, yaitu daun singkong. Tujuan penelitian ini adalah untuk menganalisis potensi ekstrak daun singkong (Manihot esculenta Crantz) terhadap ekspresi MMP-8 fibroblas pada model tikus disfungsi ovarium dan periodontitis. Metode: Penelitian ini merupakan penelitian eksperimental laboratoris dengan post-test only control group design. Sampel yang digunakan adalah tikus Sprague-Dawley betina yang dibagi dalam 5 kelompok yaitu (1) kelompok kontrol (K), (2) Kelompok yang diinduksi bakteri Porphyromonas gingivalis dan diberi aquades, (3) Kelompok yang diinduksi bakteri Porphyromonas gingivalis dan diberi ekstrak daun singkong, (4) Kelompok yang diberi perlakuan ovariektomi dan diberi aquades, (5) Kelompok yang dilakukan ovariektomi dan diberi ekstrak daun singkong. Pengambilan jaringan gingiva setelah tahap euthanasia dilakukan untuk pembuatan preparat histopatologi dengan pewarnaan imunohistokimia. Pengamatan dan penghitungan ekspresi MMP-8 dilakukan dengan menggunakan software ImageJ dan Immunoratio. Hasil: Hasil analisis data one-way ANOVA, ekspresi MMP-8 fibroblas gingiva menunjukkan perbedaan yang signifikan (p=0,000). Hal ini menunjukkan bahwa ekstrak daun singkong dapat menurunkan ekspresi MMP-8 fibroblas gingiva tikus yang mengalami disfungsi ovarium dan periodontitis diinduksi P. gingivalis. Simpulan: Ekstrak daun singkong (Manihot esculenta Crantz) dapat menurunkan ekspresi MMP-8 sel fibroblas gingiva pada model tikus disfungsi ovarium dan periodontitis.Kata kunci: Disfungsi ovarium, periodontitis, ekspresi MMP-8, ekstrak daun singkong. ABSTRACTIntroduction: Ovarian dysfunction is a condition that causes estrogen and progesterone deficiency. This hormonal decrease causes an increase in the production of Tumour Necrosis Factor (TNF), which can trigger an increase in the production of matrix metalloproteinase-8 (MMP-8). Periodontitis caused by gram-negative bacteria will trigger macrophages to release TNF-α, which contributes to the formation of MMP-8. MMP-8 plays a role in collagen degradation of the gingival connective tissue. An increase in MMP-8 can cause periodontitis. Periodontitis symptoms due to ovarian dysfunction and bacterial induction can be slowed down by natural ingredients, such as cassava leaf. The purpose of this study was to analyse the potential of cassava leaf extract (Manihot esculenta Crantz) on MMP-8 expression of gingival fibroblast in rats model with ovarian dysfunction and periodontitis. Methods: This study was an experimental laboratory with post-test only control group design. The samples were female Sprague-Dawley rats divided into five groups: (1) control group (K); (2) Porphyromonas gingivalis induced group and given aquadest; (3) Porphyromonas gingivalis induced and given cassava leaf extract; (4) Group with ovariectomy treatment and given aquadest; (5) Group with ovariectomy treatment and given cassava leaf extract. Gingival tissue retrieval after the euthanasia was carried out for the histopathology preparations by immunohistochemical staining. Observation and calculation of MMP-8 expressions were performed using ImageJ and Immunoratio software. Results: The results of the one-way ANOVA analysis of MMP-8 expression of gingival fibroblasts showed a significant difference (p = 0.000); thus cassava leaf extract reduce the MMP-8 expression of gingival fibroblasts of rats with ovarian dysfunction and P. gingivalis induced periodontitis. Conclusion: Cassava (Manihot esculenta Crantz) leaf extract can reduce the MMP-8 expression of gingival fibroblast cells in rats’ model with ovarian dysfunction and periodontitis.Keywords: Ovarian dysfunction, periodontitis, MMP-8 expression, cassava leaf extract.
25
Wardi, Ully Nafisah. "Viability Assay Of Human Fibroblast Cells Treated by Water Hyacinth Leaf Extract After 24 Hours Incubation." Journal of Stem Cell Research and Tissue Engineering 5, no. 2 (January 26, 2022): 87. http://dx.doi.org/10.20473/jscrte.v5i2.33147.
Abstract:
Inflammation and alveolar bone resorption are indications of periodontal disease, which is a chronic inflammatory illness caused by bacterial colonization that damages the soft and hard structures that support the teeth. In response to persistent tissue injury and chronic inflammation, fibroblasts also play a role in the synthesis and maintenance of extracellular matrix, cell proliferation, and cell differentiation. Fibroblasts play a crucial part in the healing of wounds. Phenols, alkaloids, flavonoids, and tannins are some of the health-promoting components found in water hyacinth. As a result, plant extracts must be tested first, one of which is the viability test in accordance with the requirements and materials in the field of dentistry. The viability test is a cell-based test that is often used for screening compounds to determine whether the test compound has an effect on cell proliferation or has a direct cytotoxic effect that leads to cell death. The goal of this study is to figure out what concentration of water hyacinth leaf extract can keep human gingival fibroblast cells alive for 24 hours. Primary cell cultures from human gingiva were extracted and placed in a 96-well microplate. For 24 hours, water hyacinth leaf extract at concentrations of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.25 mg/ml, 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, 0.0156 mg/ml was administered to each well in the microplate. After 24 hours of incubation, the MTT assay was carried out by adding MTT solution. The optical density of formazan was measured using an ELISA reader at a wavelength of 590 nm, and viability was calculated using the viability formula. Starting at 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, and 0.0156 mg/ml, the vitality of human gingival fibroblast cells was good. In the treatment group, the greatest vitality of human gingival fibroblast cells was 0.0156 mg/ml (75.98%).
26
Smith, P. C., and J. Martínez. "Differential uPA Expression by TGF-β1 in Gingival Fibroblasts." Journal of Dental Research 85, no. 2 (February 2006): 150–55. http://dx.doi.org/10.1177/154405910608500207.
Abstract:
Transforming Growth Factor-β1 (TGF-β1) plays a key role in connective tissue remodeling and inflammation. Under pathological conditions, like periodontal disease, fibroblasts may display an altered response to this growth factor. To investigate this question, we have studied whether TGF-β1 may differentially regulate the expression of urokinase at the protein level in primary cultures of fibroblasts derived from healthy gingiva, granulation tissue from gingival wounds, and chronic periodontal disease. We observed that TGF-β1 may repress urokinase expression in healthy gingival fibroblasts and promote its production in granulation-tissue fibroblasts. A significant correlation was found between expression of the myofibroblast marker α-smooth-muscle actin and stimulation of urokinase production by TGF-β1. Immunostaining of gingival wounds showed that myofibroblasts were involved in urokinase production. TGF-β1-stimulated urokinase expression was blocked after inhibition of the c-jun-NH2 terminal kinase signaling pathway. We propose that stimulation of urokinase production by TGF-β1 is involved in the responses of activated fibroblasts to tissue injury.
27
Zavarella, M. M., O. Gbemi, and J. D. Walters. "Accumulation of Non-steroidal Anti-inflammatory Drugs by Gingival Fibroblasts." Journal of Dental Research 85, no. 5 (May 2006): 452–56. http://dx.doi.org/10.1177/154405910608500511.
Abstract:
Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblasts transport naproxen in a saturable, temperature-dependent manner with a Km of 127 μg/mL and a Vmax of 1.42 ng/min/μg protein. At steady state, the intracellular/extracellular concentration ratio was 1.9 for naproxen and 7.2 for ibuprofen. Naproxen transport was most efficient at neutral pH and was significantly enhanced upon cell treatment with TNF-α. In humans, systemically administered naproxen attained steady-state levels of 61.9 μg/mL in blood and 9.4 μg/g in healthy gingival connective tissue, while ibuprofen attained levels of 2.3 μg/mL and 1.5 μg/g, respectively. Thus, gingival fibroblasts possess transporters for NSAIDs that are up-regulated by an inflammatory mediator, but there is no evidence that they contribute to elevated NSAID levels in healthy gingiva.
28
Azab, Ehab, and Abdel-Rahman Youssef. "Biocompatibility Evaluation of Human and Porcine Acellular Dermal Matrix on Human Primary Gingival Fibroblasts: In Vitro Comparative Study." European Journal of Dentistry 15, no. 03 (June 18, 2021): 563–67. http://dx.doi.org/10.1055/s-0041-1727551.
Abstract:
Abstract Objective Allogeneic and xenogeneic acellular dermal matrix (ADM) grafts have been used to treat periodontal soft tissue defects. The purpose of the current study was to compare the effect of human ADM (AlloDerm) and porcine ADM (Derma) on human primary gingival fibroblasts in vitro regarding the biocompatibility test. Materials and Methods Gingival fibroblasts were obtained from healthy adult gingiva and seeded on AlloDerm or Derma ADM in 96-well plate. The control cells were grown on a surface-treated polystyrene cell-culture plate without matrix. The cells were cultured for 3, 7, and 14 days. The fibroblasts morphology was examined using inverted microscopy, and the cell viability of fibroblasts adherent to the dermal matrix was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay after 3, 7, and 14 days in culture. The data were statistically evaluated by one-way analysis of variance. p-Value of 0.05 was considered significant. Results Gingival fibroblasts adjacent to the AlloDerm and Derma matrices were healthy, attached to the well, and did not exhibit any cytopathic changes similar to control. There were no statistically significant differences in the cell viability between the gingival fibroblasts attached to Derma and AlloDerm on day 3 (p = 0.841), day 7 (p = 0.198), and day 14 (p = 0.788). Conclusion Considering this in vitro study’s limitations, both human and porcine ADM were compatible with the surrounding human primary gingival fibroblasts. No significant differences were observed in the cell viability between the gingival fibroblasts that were attached to Derma and AlloDerm.
29
Bonson, S., B. G. Jeansonne, and T. E. Lallier. "Root-end Filling Materials Alter Fibroblast Differentiation." Journal of Dental Research 83, no. 5 (May 2004): 408–13. http://dx.doi.org/10.1177/154405910408300511.
Abstract:
Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation. Abbreviations: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).
30
Ju, Yanqin, Lijuan Huang, Shuwei Wang, and Shouliang Zhao. "Transcriptional Analysis Reveals Key Genes in the Pathogenesis of Nifedipine-Induced Gingival Overgrowth." Analytical Cellular Pathology 2020 (May 13, 2020): 1–11. http://dx.doi.org/10.1155/2020/6128341.
Abstract:
Background. Nifedipine-induced gingival overgrowth (NGO) is a multifactorial pathogenesis with increased extracellular matrix including collagen and glycans, inflammatory cytokines, and phenotype changes of fibroblasts. However, the molecular etiology of NGO is not well understood. The objective of this study is to investigate the key genes in the pathogenesis of NGO. Methods. In this study, we examined the proliferation and migration abilities of fibroblasts derived from patients with chronic periodontitis, nifedipine nonresponder gingival overgrowth, gingival overgrowth caused by nifedipine, and healthy normal gingiva. We conducted RNA-Seq on these four groups of fibroblasts and analysed the differentially expressed genes (DEGs). Results. Fibroblasts derived from NGO patients had higher proliferation and migration abilities than those of the other groups. Protein-protein interaction network analysis indicated that TGFB2, ITGA8, ITGA11, FGF5, PLA2G4D, PLA2G2F, PTGS1, CSF1, LPAR1, CCL3, and NKX3-1 are involved in the development of NGO. These factors are related to the arachidonic acid metabolism and PI3K/AKT signaling pathways. Conclusion. Transcriptional gene expression analysis identified a number of DEGs that might be functionally related to gingival overgrowth induced by nifedipine. Our study provides important information on the molecular mechanism underlying nifedipine-induced gingival overgrowth.
31
Beklen, A., G. Tüter, T. Sorsa, R. Hanemaaijer, I. Virtanen, T. Tervahartiala, and Y. T. Konttinen. "Gingival Tissue and Crevicular Fluid Co-operation in Adult Periodontitis." Journal of Dental Research 85, no. 1 (January 2006): 59–63. http://dx.doi.org/10.1177/154405910608500110.
Abstract:
Activated matrix metalloproteinase-3 (MMP-3) can contribute to periodontal ligament destruction in adult periodontitis. Since MMP-3 has been reported to activate proMMP-8 and -9, it was speculated that gingival tissue fibroblast-derived MMP-3 might, in periodontitis, be responsible for activation of gingival crevicular fluid (GCF) neutrophil-derived proMMP-8 and -9. Immunohistochemistry disclosed MMP-3 in gingival fibroblasts in periodontitis. Cultured gingival fibroblasts released only pro-MMP-3 when stimulated with tumor necrosis factor-α. However, Western blot revealed partially activated MMP-3, MMP-8, and MMP-9 in periodontitis GCF. Active MMP-8 (p < 0.05) and MMP-9 (p < 0.05) correlated with the presence of active MMP-3. It seems that resident gingival fibroblasts produce pro-MMP-3 in GCF, where it becomes activated, probably by cathepsin G or elastase released by neutrophils. Active MMP-3 then activates neutrophil-derived pro-MMP-8 and -9. Different tissue compartments/cells exert co-operative actions in mutual local MMP activation cascades.
32
Liao, James, Jabrane Azelmat, Lei Zhao, Masami Yoshioka, Daisuke Hinode, and Daniel Grenier. "The Kampo Medicine Rokumigan Possesses Antibiofilm, Anti-Inflammatory, and Wound Healing Properties." BioMed Research International 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/436206.
Abstract:
Periodontal diseases, which are inflammatory diseases of bacterial origin affecting the tooth-supporting tissues, are characterized by inflammation and destruction of gingival connective tissue and alveolar bone, and may lead to tooth loss. The aim of the study was to investigate Rokumigan, a Kampo Japanese traditional medicine made of six different plants, for its capacity to prevent biofilm formation byFusobacterium nucleatum, to inhibit interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion by mucosal cells, and to promote wound healing in a fibroblast model. Using a microplate colorimetric assay, Rokumigan prevented biofilm formation byF. nucleatum, while it had no effect on bacterial growth. Rokumigan inhibited IL-6 secretion in both epithelial cells and fibroblasts stimulated with lipopolysaccharide. However, it caused no significant inhibition of IL-8 secretion by both cell types. Rokumigan significantly increased proliferation and migration of gingival fibroblasts in a wound healing assay. In conclusion, the Kampo formulation Rokumigan, through suppression of biofilm formation byF. nucleatum, inhibition of IL-6 secretion by gingival epithelial cells and fibroblasts, and promotion of wound healing in a fibroblast model, may have potential application for periodontal diseases.
33
Nixon, Connie S., Michelle J. Steffen, and Jeffrey L. Ebersole. "Cytokine Responses to Treponema pectinovorum andTreponema denticola in Human Gingival Fibroblasts." Infection and Immunity 68, no. 9 (September 1, 2000): 5284–92. http://dx.doi.org/10.1128/iai.68.9.5284-5292.2000.
Abstract:
ABSTRACT Human gingival fibroblasts were challenged with Treponema pectinovorum and Treponema denticola to test three specific hypotheses: (i) these treponemes induce different cytokine profiles from the fibroblasts, (ii) differences in cytokine profiles are observed after challenge with live versus killed treponemes, and (iii) differences in cytokine profiles are noted from different gingival fibroblast cell lines when challenged with these treponemes. Three normal gingival fibroblast cell cultures were challenged withT. pectinovorum and T. denticola strains, and the supernatants were analyzed for cytokine production (i.e., interleukin-1α [IL-1α], IL-1β, IL-6, IL-8, IL-10, gamma interferon, macrophage chemotactic protein 1 [MCP-1], platelet-derived growth factor, tumor necrosis factor alpha, and granulocyte-macrophage colony-stimulating factor). Unstimulated fibroblast cell lines produced IL-6, IL-8, and MCP-1. T. pectinovorum routinely elicited the greatest production of these cytokines from the fibroblast cell lines, increasing 10- to 50-fold over basal production. While T. denticola also induced IL-6 and IL-8 production, these levels were generally lower than those elicited by challenge with T. pectinovorum. MCP-1 levels were significantly lower after T. denticola challenge, and the kinetics suggested that this microorganism actually inhibited basal production by the fibroblasts. No basal or stimulated production of the other cytokines was observed. Significant differences were noted in the responsiveness of the various cell lines with respect to the two species of treponemes and the individual cytokines produced. Finally, dead T. pectinovorum generally induced a twofold-greater level of IL-6 and IL-8 than the live bacteria. These results supported the idea that different species of oral treponemes can elicit proinflammatory cytokine production by gingival cells and that this stimulation did not require live microorganisms. Importantly, a unique difference was noted in the ability of T. pectinovorum to induce a robust MCP-1 production, while T. denticolaappeared to inhibit this activity of the fibroblasts. While the general cytokine profiles of the fibroblast cell cultures were similar, significant differences were noted in the quantity of individual cytokines produced, which could relate to individual patient variation in local inflammatory responses in the periodontium.
34
Pham, Maria H., Håvard J. Haugen, Alessandra Rinna, Jan Eirik Ellingsen, and Janne E. Reseland. "Hydrofluoric acid treatment of titanium surfaces enhances the proliferation of human gingival fibroblasts." Journal of Tissue Engineering 10 (January 2019): 204173141982895. http://dx.doi.org/10.1177/2041731419828950.
Abstract:
The attachment of implants relies on bone and soft tissue biocompatibility. The aim of this article is to investigate the effect of fluoride-modified metallic titanium (Ti) surfaces (Ti-F) on proliferation and differentiation of human gingival fibroblasts. Human gingival fibroblast cells were exposed to hydrofluoric acid–modified Ti coins (Ti-F) for 1, 3, 7, 14 and 21 days, and untreated coins were used as controls. A five- to six-fold increase in the proliferation of human gingival fibroblasts on Ti-F compared to Ti surfaces was observed. Enhanced gene expression of interleukin-6 and osteoprotegerin was found at 7 days. Increased levels of sclerostin, interleukin-6 and osteoprotegerin in the media from human gingival fibroblasts cultured on Ti-F coins were found compared to controls. Our results confirm that hydrofluoric acid–modified surface may indirectly enhance the firm attachment of implant surface to junction epithelium, soft tissue epithelium, which would give protection for underlying osseous structures making osseointegration of the dental implant possible.
35
Fauzia, Malianawati, and Audia Putri Dewanti. "The effect of lime (Citrus Aurantifola Swingle) peel extract in periodontal dressings on the number of fibroblasts in the gingival wound healing process." Dental Journal (Majalah Kedokteran Gigi) 55, no. 2 (June 1, 2022): 81–87. http://dx.doi.org/10.20473/j.djmkg.v55.i2.p81-87.
Abstract:
Background: Periodontal dressing commonly used in dentistry today does not contain compounds that can accelerate wound healing. Lime (Citrus aurantifolia Swingle) peel contains flavonoids that play a role in increasing fibroblast cells so that they can accelerate the healing process. Periodontal dressings supplemented with lime (Citrus aurantifolia Swingle) peel extract are expected to provide an alternative material that can accelerate wound healing in addition to closure. Purpose: The study aims to determine the effect of adding lime (Citrus aurantifolia Swingle) peel extract to periodontal dressings on the increase in the number of fibroblasts in the gingival healing process. Methods: The study was conducted in an experimental laboratory in vivo. The study used a post-randomised control group of 32 rabbits with lesions of the mandibular gingiva using a 2 mm diameter punch biopsy. The experimental animals were divided into 8 groups, namely the control group, which was treated with periodontal dressings without the addition of lime (Citrus aurantifolia Swingle) peel extract, and the treatment group, which was treated with periodontal dressings with the addition of the extract. Histological observations of the tissues were performed with HE staining to count the number of fibroblasts. Results: Statistical test results showed that there was a significant difference in the number of fibroblasts between the control group and the treatment group on day 3 and day 5 (ANOVA, p <0.05). Conclusion: Adding extra lime (Citrus aurantifolia Swingle) peel to the periodontal dressing increases the number of fibroblast cells after gum injury.
36
Fauzia, Malianawati, and Audia Putri Dewanti. "The effect of lime (Citrus Aurantifola Swingle) peel extract in periodontal dressings on the number of fibroblasts in the gingival wound healing process." Dental Journal (Majalah Kedokteran Gigi) 55, no. 2 (June 1, 2022): 81–87. http://dx.doi.org/10.20473/j.djmkg.v55.i2.p81-87.
Abstract:
Background: Periodontal dressing commonly used in dentistry today does not contain compounds that can accelerate wound healing. Lime (Citrus aurantifolia Swingle) peel contains flavonoids that play a role in increasing fibroblast cells so that they can accelerate the healing process. Periodontal dressings supplemented with lime (Citrus aurantifolia Swingle) peel extract are expected to provide an alternative material that can accelerate wound healing in addition to closure. Purpose: The study aims to determine the effect of adding lime (Citrus aurantifolia Swingle) peel extract to periodontal dressings on the increase in the number of fibroblasts in the gingival healing process. Methods: The study was conducted in an experimental laboratory in vivo. The study used a post-randomised control group of 32 rabbits with lesions of the mandibular gingiva using a 2 mm diameter punch biopsy. The experimental animals were divided into 8 groups, namely the control group, which was treated with periodontal dressings without the addition of lime (Citrus aurantifolia Swingle) peel extract, and the treatment group, which was treated with periodontal dressings with the addition of the extract. Histological observations of the tissues were performed with HE staining to count the number of fibroblasts. Results: Statistical test results showed that there was a significant difference in the number of fibroblasts between the control group and the treatment group on day 3 and day 5 (ANOVA, p <0.05). Conclusion: Adding extra lime (Citrus aurantifolia Swingle) peel to the periodontal dressing increases the number of fibroblast cells after gum injury.
37
Lauritano, Dorina, Giulia Moreo, Luisa Limongelli, Annalisa Palmieri, and Francesco Carinci. "Drug-Induced Gingival Overgrowth: The Effect of Cyclosporin A and Mycophenolate Mophetil on Human Gingival Fibroblasts." Biomedicines 8, no. 7 (July 17, 2020): 221. http://dx.doi.org/10.3390/biomedicines8070221.
Abstract:
Drug-induced gingival overgrowth may occur after a chronic administration of three classes of systemic drugs: Anticonvulsants, immunosuppressants, and calcium channel blockers. This study aimed to investigate how cyclosporin A and mycophenolate mophetil (immunosuppressive drugs) could interfere with human gingival fibroblasts functions, leading to gingival enlargement. Human gingival fibroblasts derived from the tissue of a 60-year-old female were cultured in a DMEME medium. A stock solution with 1 mg/mL of mycophenolate and 1 mg/mL of cyclosporine were prepared and dissolved in a DMEM medium to prepare a serial dilution at the concentrations of 5000, 2000, 1000, 500, and 100 ng/mL, for both treatments. Cell viability was measured using the PrestoBlue™ Reagent Protocol. Quantitative real-time RT-PCR was performed in order to analyze the expression of 57 genes coding for gingival fibroblasts “Extracellular Matrix and Adhesion Molecules”. Mycophenolate and cyclosporine had no effect on fibroblast cell viability at 1000 ng/mL. Both the treatments showed similar effects on the expression profiling of treated cells: Downregulation of most extracellular matrix metalloproteases genes (MMP8, MMP11, MMP15, MMP16, MMP24) was assessed, while CDH1, ITGA2, ITGA7, LAMB3, MMP12, and MMP13 were recorded to be upregulated in fibroblasts treated with immunosuppressive drugs. It has been demonstrated that gingival overgrowth can be caused by the chronic administration of cyclosporin A and mycophenolate mophetil. However, given the contrasting data of literature, further investigations are needed, making clear the possible effects of immunosuppressive drugs on fibroblasts.
38
Sardi, Ni Wayan Arni, Ni Luh Putu Sri Maryuni Adnyasari, and Ni Putu Ratih Berliana Ekasari. "GEL EXTRACTION OF EARTHWORMS (Lumbricus rubellus) TO THE NUMBER OF FIBROBAL CELLS IN MALE WISTAR RATS (Rattus norvegiccus) GINGIVAL WOUND HEALING." Interdental Jurnal Kedokteran Gigi (IJKG) 19, no. 1 (June 23, 2023): 34–42. http://dx.doi.org/10.46862/interdental.v19i1.6096.
Abstract:
Introduction: Gingiva is one of the oral mucosa that is most susceptible to injury, one of the factors is surgical procedures such as curettage which will be followed by a natural wound healing process. When a wound occurs, the cells that act as the building blocks of ground substance and the formation of collagen fibers in closing the wound are fibroblast cells. Many people think of earthworms (Lumbricus rubellus) only as animal feed or fish feed, but earthworms have many properties in antiperetic, antispasmodic, antidiuretic, antiasmatic, antihypertensive, antiallergic, anti-inflammatory, and have fibrinolytic activity. The purpose of this study was to determine the effect of the application of earthworm (Lumbricus rubellus) extract gel on the number of fibroblast cells in the gingival wound healing process of male Wistar (Rattus norvegicus) rats. Materials and Methods : This research method used in vivo laboratory experiments on wistar rats with three treatment groups, namely the group receiving 80% earthworm extract gel, and the control group giving placebo gel (CMC-Na 2%) which was observed on day 3. Results and Discussion: The results showed that there was a significant difference in the mean score of the number of fibroblasts on day 3 in the treatment group and the control group. The treatment group was given an 80% concentration of earthworm (Lumbricus rubellus) extract gel, which was 418.67, while the control group on day 3 was 270.33. Conclusion: It can be concluded that the gel extract of earthworm (Lumbricus rubellus) concentrate 80% is effective in increasing the number of fibroblast cells in the healing process of rat gingival wounds.
39
Binderman, I., H. Bahar, J. Jacob-Hirsch, S. Zeligson, N. Amariglio, G. Rechavi, S. Shoham, and A. Yaffe. "P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery." Journal of Dental Research 86, no. 2 (February 2007): 181–85. http://dx.doi.org/10.1177/154405910708600214.
Abstract:
Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hypothesized that local release of ATP signaling through P2X4 elicits activation of osteoclasts on the alveolar bone surface. In this study, we identified intense immunoreactivity of gingival fibroblasts to P2X4-specific antibodies and a 6.4-fold increase in expression by real-time RT-PCR. Moreover, a single local application, at the time of surgery, of Apyrase (which degrades ATP) or Coomassie Brilliant Blue (an antagonist of purinoreceptors) significantly reduced alveolar bone loss. We propose that ATP flowing from cells after surgery can directly activate P2X4 receptors in the sensor cells of marginal gingiva through Ca2+ signaling, or by direct activation of osteoclasts on the bone surface.
40
Bramanti, I., ISR Sudarso, MSH Wahyuningsih, T. Wibawa, VM Karina, and B. Kusumawardani. "Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro." Bangladesh Journal of Medical Science 17, no. 4 (September 19, 2018): 556–61. http://dx.doi.org/10.3329/bjms.v17i4.38315.
Abstract:
Introduction: Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory effect. Moreover, garlic extract has a good biocompatibility and can stimulate cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied.Objective: The aim of this study was to analyze the biocompatibility of garlic extract by observing the viability and proliferation of human gingival fibroblasts in vitro.Methods: Biocompatibility test was conducted using serial concentration of garlic extract. Human gingival fibroblasts was seeded into 96 microwell plate with density of 2x103 cells, added with the fourteen serial concentration of garlic extract, and incubated in 37o C and 5% CO2for 24, 48 and 72 hours. MTT assay was used to analyze the viability and proliferation of human gingival fibroblasts. Data were analyzed by the Kruskal Wallis and U Mann-Whitney test.Results: The result showed that in each time of observation, there is no significant difference in viability fibroblast (p>0,05), but there are significant difference between time of observation at 24, 48, and 72 hours (p <0.05).Data showed that all concentration of garlic extract increased the viability and proliferation of human gingival fibroblasts.Conclusions: The ethanolic garlic extract has a good biocompatibility to human gingival fibroblasts culture cell and can stimulate the proliferation of human gingival fibroblast.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.556-561
41
Tsuruga, E., K. Irie, and T. Yajima. "Fibrillin-2 Degradation by Matrix Metalloproteinase-2 in Periodontium." Journal of Dental Research 86, no. 4 (April 2007): 352–56. http://dx.doi.org/10.1177/154405910708600410.
Abstract:
Elastic system fibers, comprised of microfibrils and tropoelastin, are extracellular components of periodontal tissue. During development, the microfibrils act as a template on which tropoelastin is deposited. However, the process of elastic system fiber remodeling is not fully understood. Therefore, we examined whether matrix metalloproteinases (MMPs) are involved in the remodeling of fibrillins (major components of microfibrils) by human gingival fibroblasts and periodontal ligament (PDL) fibroblasts. Gingival and PDL fibroblasts were cultured for 6 weeks. In some cultures, MMP inhibitor or tissue inhibitor of matrix metalloproteinsase-2 (TIMP-2) was added to the medium for an additional 2 weeks. Active MMP-2 (62 kDa) appeared as cell-membrane-associated or in extracellular matrix only in PDL fibroblast cell layers. The addition of MMP inhibitor or TIMP-2 significantly increased fibrillin-2 accumulation in PDL fibroblast cell layers, and decreased the amount of fibrillin-2 fragments, suggesting that active MMP-2 may degrade fibrillin-2, and that MMPs may play a role in the remodeling of elastic system fibers in PDL.
42
Sulistiawati, Sulistiawati, Hema Awalia, Ulfa Yasmin, Rosada Sintya Dwi, and Alya Namira. "THE EFFECT OF SEMENDO COFFEE ON THE NUMBER OF FIBROBLAST CELLS IN GINGIVA WOUND OF RATS." B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah 9, no. 1 (June 12, 2022): 19–25. http://dx.doi.org/10.33854/jbd.v9i1.932.
Abstract:
Introduction: Gingiva is a keratinized epithelium tissue that surrounds the tooth and protects the tissue beneath it, which is often wounded. Sumatran robusta coffee, semendo coffee, consists of active compounds such as polyphenol, alkaloid, and saponin that can increase fibroblast amount during the wound healing process. This study aims to determine the effect of semendo coffee (Coffea canephora) extract on fibroblast amount on gingiva wound of Rattus norvegicus. Methods: The true experimental study with a post-test-only control group design was confirmed in this study. This study used twenty-four rats (Rattus norvegicus) divided into 4 groups. The gingival injury was performed on mandible gingiva using a punch biopsy method with a 2mm diameter. The gel was given according to the treatment group 2 times a day for 7 days. Semendo coffee extract at 5%, 20%, and 40%, was applied to the wound treatment groups, while placebo gel was applied to the control group. Euthanasia was delivered on the 8th day, then histological preparation was made. The amount of fibroblast was analyzed by Olympus software. Result: The results showed that the Semendo coffee extract could significantly improve the number of fibroblast cells compared to the control groups. The highest fibroblast amount was found in the group with Semendo coffee extract at a concentration of 40%. Conclusion: Semendo coffee extract at 5%, 20%, and 40% increased the amount of fibroblast on the gingival wound in Rattus norvegicus.
43
Kato, T., T. Imatani, T. Miura, K. Minaguchi, E. Saitoh, and K. Okuda. "Cytokine-Inducing Activity of Family 2 Cystatins." Biological Chemistry 381, no. 11 (November 15, 2000): 1143–47. http://dx.doi.org/10.1515/bc.2000.141.
Abstract:
AbstractCystatins are physiological cysteine proteinase inhibitors. Here we report a novel function for some family 2 cystatins that is not related to these activities. The release of interleukin-6 (IL-6) and interleukin-8 (IL-8) by the gingival fibroblasts and that of IL-6 by murine splenocytes were measured using ELISA systems specific for these cytokine molecules. Family 2 cystatins, including cystatins C, SA1, SA2, S, and egg white cystatin, upregulated the IL-6 production by two human gingival fibroblast cell lines or murine splenocytes and also the IL-8 production by gingival fibroblasts at physiological concentrations. After complete saturation with papain, those family 2 cystatins still upregulated IL-6 production, suggesting that the papain- inhibitory site was not involved in the cytokine-inducing activity.
44
Yajima, T. "Collagen Remodeling in Wound Healing by Gingival Fibroblasts in Vitro." Advances in Dental Research 2, no. 2 (November 1988): 228–33. http://dx.doi.org/10.1177/08959374880020020601.
Abstract:
Collagen degradation by fibroblasts was studied in the absence of other cell types to improve our understanding of the mechanisms by which fibroblasts digest collagen. Human gingival fibroblasts were cultured in α -MEM medium for eight weeks. Incisional wounds were made in the fibroblast cultures, and the cells were fixed by different procedures at two days post-wounding. Collagen remodeling has been investigated by tracer experiments and by cytochemical demonstration of acid and alkaline phosphatase activity at the ultrastructural level and stereological analysis in experimental wound-healing in vitro. The results showed that fibroblasts in the wounded zone exhibited high collagen phagocytic activity, and indicate that fibroblasts have a fundamental role to play in collagen remodeling in wound repair in vitro. This in vitro experimental system is also suggested as a useful model for the analysis of collagen remodeling in wound-healing by fibroblasts.
45
Zeldich, E., R. Koren, C. Nemcovsky, and M. Weinreb. "Enamel Matrix Derivative Stimulates Human Gingival Fibroblast Proliferation via ERK." Journal of Dental Research 86, no. 1 (January 2007): 41–46. http://dx.doi.org/10.1177/154405910708600106.
Abstract:
Emdogain®, a formulation of Enamel Matrix Proteins, is used clinically for periodontal regeneration to stimulate PDL (periodontal ligament), cementum, and bone formation. Its effects on gingival fibroblasts and tissue have not been thoroughly studied. Therefore, we investigated the mechanisms by which Emdogain affects the cell cycle of human gingival fibroblasts. Without serum, Emdogain (50 μg/mL) induced human gingival fibroblast entry into the S phase and DNA synthesis, but not completion of the cell cycle. With low serum concentrations (0.2–0.5%), Emdogain synergistically induced completion of the cell cycle, resulting in increased cell numbers. The mitogenic response to Emdogain depended on Extracellular Regulated Kinase (ERK) activation, which occurred in two waves, peaking after 15 min and 4 to 6 hrs, since it was abolished by U0126, a specific MAPK inhibitor. Inhibition of the second wave was sufficient to abrogate mitogenesis. This study characterized the mitogenic effect of Emdogain on primary human gingival fibroblasts, its cooperation with serum growth factors, and the key mediatory role of the ERK cascade.
46
Larjava, H., J. Heino, T. Krusius, E. Vuorio, and M. Tammi. "The small dermatan sulphate proteoglycans synthesized by fibroblasts derived from skin, synovium and gingiva show tissue-related heterogeneity." Biochemical Journal 256, no. 1 (November 15, 1988): 35–40. http://dx.doi.org/10.1042/bj2560035.
Abstract:
Dermatan sulphate proteoglycans (DSPGs) synthesized in the presence of 35SO4 were characterized in culture media of fibroblast lines obtained from skin, synovium, and gingiva. The molecular mass of DSPG varied from 95-130 kDa as estimated by SDS/polyacrylamide-gel electrophoresis. Gingival fibroblasts constantly produced larger DSPGs than skin fibroblasts. This was due to the larger dermatan sulphate (DS) chains, which also showed tissue-related heterogeneity in the distribution of 4- and 6-sulphated disaccharide units. The N-glycosylated cores (44 and 47 kDa) obtained following chondroitinase ABC treatment were of identical size in all tissues. The cores from the different tissues were also of the same size (38 kDa) when addition of the N-linked oligosaccharides was inhibited by tunicamycin or when they were removed by N-glycanase treatment. No evidence for low-molecular-mass sulphated oligosaccharides was found. All tissues contained two mRNA species (1.6 and 1.9 kb) for the DSPG core protein. These data suggest that the pattern of transferase activities involved in the construction of DS chains differs from one tissue to another. This variation may modulate the functions of DSPG in the extracellular matrix.
47
Vahabi, Surena, Bahareh Nazemi salman, and Pouya Pourgolshani. "Effect of Phenytoin and Cyclosporine on IL-17 Production by Gingival Fibroblasts of Adults and Children." Journal of Periodontology & Implant Dentistry 7, no. 1 (October 8, 2018): 1–6. http://dx.doi.org/10.15171/jpid.2015.001.
Abstract:
Background and aims. Gingival hyperplasia, a relatively common side effect of antiepileptic and anticonvulsant drugs, occurs in 30‒50% of patients taking phenytoin and 25‒81% of those taking cyclosporine. Gingival hyperplasia due to lack of balance between extracellular synthesis and degradation is associated with increased production of IL-1B, IL-6 and IL-8 by gingival fibroblasts. Tissue level of IL-17 increases in inflammatory conditions. Since the role of IL-17 and patient age in gingival hyperplasia is still unclear, this study aimed to compare the level of IL-17 produced by gingival fibroblasts in children and adults. Materials and methods. This study was conducted on biopsy specimens obtained from the healthy gingiva of 4 adults, 35‒42 years of age, undergoing crown lengthening surgery and 4 children, aged 4‒11 years, undergoing impacted tooth surgery. Biopsy specimens were cultured in a mixture of Dulbecco’s Modified Eagle’s Medium (DMEM), penicillin, 1% streptomycin and 10% fetal bovine serum (FBS) at 37°C and 5% CO2. The specimens were monitored for contamination and cell proliferation and the medium was refreshed if necessary. Results. The baseline levels of IL-17 produced by gingival fibroblasts isolated from children and adults were not significantly different from those after the addition of cyclosporine or phenytoin. The two groups of children and adults were not significantly different in terms of the production of IL-17 by gingival fibroblasts. The two groups of children and adults were not significantly different in terms of the production of IL-17 at baseline or after exposure to cyclosporine or phenytoin. Conclusion. IL-17 inflammatory cytokine does not play a role in gingival hyperplasia in children and adults.
48
Nowak-Terpiłowska, Agnieszka, Izabela Nowak, Agnieszka Feliczak-Guzik, and Marzena Wyganowska. "Analysis of the Impact of Ethanol Extract of Calendula officinalis L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells." Life 13, no. 10 (September 22, 2023): 1949. http://dx.doi.org/10.3390/life13101949.
Abstract:
Calendula officinalis L. promotes wound healing and might be effective in gingival fibroblast stimulation. The influence of different concentrations of Calendula officinalis L. ethanol extract on human gingival fibroblast was visualized using PANsys 3000—a fully automated cell culture device used for in vitro culture to study cells under conditions similar to in vivo. The human fibroblast cells were isolated from gingival tissue. The 100% brew of Calendula officinalis L., as well as 7% and 20% Calendula officinalis L. ethanol extract, were added to the cultured cells and observed for 72 h. The qualitative and quantitative composition of the volatile compounds of marigold Calendula officinalis L. flowers are presented in this study. The essential oil compounds of the decoction were isolated with solid-phase microextraction (SPME) and analyzed with gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The presence of terpenoids, flavonoids, and other compounds was demonstrated. The composition was correlated with the fragrance properties. Observation of gingival fibroblast showed that there were no changes in cell morphology and proliferation after 100% Calendula officinalis L. brew stimulation. The growth and cell division were not inhibited. Likewise, the addition of 7% or 20% ethanol in water extract of Calendula officinalis L. stimulation did not inhibit the fibroblast proliferation. Overall, ethanol extracts of Calendula officinalis L. decrease the alcohol cytotoxic influence on gingival fibroblasts.
49
Kumar, Raghunandha. "Effect of ascorbic acid on cellular respiration with mitochondrial reductase in gingival fibroblast." Bioinformation 19, no. 5 (May 30, 2023): 552–55. http://dx.doi.org/10.6026/97320630019552.
Abstract:
Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. Therefore, it is of interest to investigate the effect of vitamin C on gingival fibroblast behavior utilizing cell culture. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. Cell migration, cell viability was assessed using MTT assay. The viability assay showed >95% live cells, and no significant (P > 0.05) difference in these values was observed at different concentrations at 24 hrs. The levels of cell proliferation were not significantly different among the control and experimental groups in 24 hrs experimental time-points (p > 0.05). Vitamin C is nontoxic and can be used for further experiments to validate for clinical application. This was further confirmed with morphological examination after 24hrs incubation on control and experimental group drugs observed under the phase contrast microscope.
50
Agrawal, Ankita. "Effect of curcumin, betadine and chlorhexidine in gingival wound healing." Bioinformation 19, no. 12 (December 31, 2023): 1153–58. http://dx.doi.org/10.6026/973206300191153.
Abstract:
The effect of chlorhexidine (CHX) digluconate, Betadine (BET), curcumin (CUR) on gingival wound healing is of interest to dental practitioners. Hence, we studied the average fibroblast viability % for each of the concentrations of CUR, BET and CHX over various time durations. It was found that mean percentage of viability of fibroblasts is high in CUR and low in CHX at all time periods while the mean percentage of viability of fibroblasts in BET 1% was greater than CHX but lower than CUR at all time periods. Thus, curcumin at a concentration of 0.003% demonstrates the least cytotoxicity for fibroblasts. Hence, it is the most effective bacterial suppression, and the best wound healing.