Dissertations / Theses on the topic 'Fibroblastes gingivaux'
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1
Lorimier, Sandrine. "L'influence de l'environnement cellulaire sur le phenotype des fibroblastes gingivaux et dermiques." Paris 5, 1996. http://www.theses.fr/1996PA05M091.
2
Senni, Karim. "Effets de polysaccharides d'origine marine sur le remodelage des tissus gingivaux et dermiques." Paris 13, 2000. http://www.theses.fr/2000PA132031.
Abstract:
Le remodelage du tissu conjonctif est une phase essentielle à certains phénomènes physiologiques tels que la cicatrisation et les développements embryonnaire et post-natal. Un remodelage matriciel harmonieux nécessite un équilibre finement contrôlé entre synthèses et dégradations macromoléculaires. Toute altération de ces phénomènes conduit à des situations pathologiques ; ainsi une sur-expression des macromolécules matricielles aboutit à diverses fibroses tandis que les pathologies inflammatoires chroniques (parodontites, arthrose, emphysème) impliquent une dérégulation du potentiel protéolytique tissulaire. Les polysaccharides sulfatés tels que l'héparine, les héparanes sulfate, les dextranes fonctionnalisés ainsi que les fucanes ont de nombreux effets biologiques. Ces molécules sont capables d'agir sur le cycle cellulaire, d'influencer la synthèse de certaines macromolècules matricielles tels que les collagènes ou encore de modifier l'expression et l'activation des métalloprotéases matricielles (MMPs). Le but de nos travaux a été d'étudier l'influence des fucanes, polysaccharides pariétaux d'algues brunes, sur certains paramètres impliqués dans le remodelage tissulaire. Ainsi, nous avons pu montrer que les fucanes étaient capables : d'inhiber la prolifération des fibroblastes gingivaux en culture et à l'inverse d'augmenter celles des fibroblastes dermiques ; d'accélérer la fibrillation des collagènes dans des cultures fibroblastiques ; d'inhiber l'expression des MMPs par des fibroblastes dermiques tout en augmentant la complexation de ces protéases avec leurs inhibiteurs spécifiques (TIMPs) ; d'inhiber directement l'élastase leucocytaire tout en protégeant les fibres élastiques dermiques de l'action de cette sérine protéase. Ce travail qui a fait l'objet d'un dépôt de brevet (n°W09932099) pourrait aboutir au développement de nouveaux composés à partir d'algues brunes capables d'inhiber les effets délétères engendrés par les destructions tissulaires occasionnées lors de pathologies chroniques.
3
Naveau, Adrien. "Traitement de l'anévrysme abdominal aortique par transplantation de fibroblastes gingivaux autologues : études in vitro." Paris 5, 2007. http://www.theses.fr/2007PA05M006.
Abstract:
La formation de l’anévrisme abdominal aortique (AAA) est associée à une dégradation matricielle et une augmentation de l’activité des métalloprotéases. Nous avons voulu tester les propriétés cicatricielles quasi embryonnaires attribuées aux fibroblastes gingivaux (GF) sur ces artères ex vivo. Pour identifier les GF en cocuture, nous avons d’abord mis au point une méthodologie de marquage à partir de nanoparticules anioniques. Nous avons ensuite analysé les sécrétions de métalloprotéases (MMP)-9, MMP-1 et MMP-3 et de leur inhibiteur tissulaire (TIMP)-1, dans les cocultures de fragments d’aorte en présence de GF sur 21 jours, ainsi que dans des cocultures de cellules musculaires lisses avec des fibroblastes gingivaux, dermiques et adventitiels. Ces résultats in vitro montrent une augmentation de TIMP-1 en présence des GF qui inhibe les enzymes et préserve notamment le capital réseau élastique artériel. La transplantation de GF nous parait une approche intéressante pour traiter les AAAAbdominal aortic aneurysm (AAA) formation is associated with matrix degradation an metalloproteinases activity increase. We aimed to test the embryo-like healing propertie attributed to the gingival fibroblast (GF) on these arteries ex vivo. In order to identify the GF in coculture, we first established a labeling method from anioni nanoparticles. We have then analyzed the secretion of metalloproteinases (MMP)-9, MMP- and MMP-3, and their tissue inhibitor (TIMP)-1 in rabbit aortic rings cultured in presence o GF, and in smooth muscle cells cultured gingival, dermal and adventitial fibroblasts. These in vitro resuits do show an increase of TIMP-1 associated with GF presence, whic inhibits these enzymes and protects as well the arterial elastic network. The GF transplantation seems to us of crucial interest to treat AAA
4
Letzelter, Corinne. "Cytotoxicite activites proteolytiques des produits metaboliques de bacteries buccales vis-a-vis de fibroblastes gingivaux in vitro." Toulouse 3, 1999. http://www.theses.fr/1999TOU30092.
Abstract:
Le but de notre travail a ete d'evaluer la cytotoxicite et l'activite proteolytique des toxines liberees dans leur milieu de culture (milieu schaedler) par 5 souches de bacteries frequemment rencontrees dans l'endodonte infecte : prevotella nigrescens, peptostreptococcus micros, capnocytophaga ochracea, fusobacterium necrophorum et fusobacterium russii et 2 souches qui en sont generalement absentes : fusobacterium nucleatum polymorphum et actinobacillus actinomycetemcomitans. Les surnageants de culture de ces bacteries, dilues au 1/10 dans le milieu de culture des fibroblastes (mem + 10%svf), entrainent des inhibitions de proliferation des fibroblastes gingivaux dont l'intensite peut etre classee comme suit : 10 a 20% pour p. Nigrescens, c. Ochracea et p. Micros, 100% pour les 3 souches de fusobacterium. Le surnageant le plus toxique est celui de a. Actinomycetemcomitans (100% d'inhibition pour une dilution au 1/1000). L'activite proteolytique des surnageants bacteriens vis a vis de la fibronectine et du collagene i, principales macromolecules constituant la matrice extracellulaire des cultures de fibroblastes a confluence, a ete recherchee par immunofluorescence indirecte. Aucune activite proteolytique n'a ete detectee dans les surnageants d'a. Actinomycetemcomitans, p. Micros et c. Ochracea. Les surnageants de p. Nigrescens et f. Necrophorum ont entraine une lyse incomplete des fibrilles de collagene i. Seul l'inhibiteur de protease, pmsf, a partiellement inhibe l'activite collagenolytique de ces surnageants, suggerant la participation d'une serine protease. Une metalloprotease telle que l'elastase n'est pas impliquee et la presence de collagenase n'a pu etre demontree. Une degradation totale du reseau de fibronectine a ete observee avec les surnageants de p. Nigrescens et f. Necrophorum, elle etait plus moderee avec les 2 autres souches de fusobacterium. Ces activites proteolytique ont ete totalement inhibee par la chaleur, le svf et +/- partiellement par le pmsf selon les souches bacteriennes. La leupeptine, l'aprotinine, la pepstatine et l'inhibiteur de trypsine du soja n'ont aucun effet. L'immunodetection sur western blot des produits de proteolyse de la fibronectine a montre que les enzymes de p. Nigrescens et f. Necrophorum entrainaient la disparition des dimeres de 440 kda et l'apparition de fragments de proteolyse de 180 kda pour p. Nigrescens, 120 kda pour f. Nucleatum polymorphum et de 70 a 210 kda pour f. Necrophorum. L'ensemble des resultats est en faveur de la presence de proteases differentes dans les divers surnageants bacteriens etudies. Tous les surnageants bacteriens ont induit une chute de l'activite de la phosphatase alcaline des fibroblastes gingivaux (differencies en preosteoblastes) et une augmentation de l'activite de la phosphatase acide.
5
Giraud, Andréas. "Développement d’une approche de thérapie cellulaire de l’anévrisme de l’aorte abdominale utilisant les fibroblastes gingivaux chez la souris." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB029/document.
Abstract:
L’anévrisme de l’aorte abdominale (AAA) correspond à une dilatation progressive de l’aorte dont la complication principale, et potentiellement mortelle, est la rupture. La physiopathologie de l’AAA est complexe mais elle comporte une destruction des fibres élastiques liée à l’activité des métalloprotéinases (MMPs) matricielles, une apoptose des cellules musculaires lisses et une infiltration inflammatoire chronique. Actuellement, il n’existe aucun traitement pharmacologique permettant de limiter la progression et la rupture de l’AAA. Les fibroblastes gingivaux (FGs) sont des cellules multipotentes anti-inflammatoires qui permettent une réparation parfaite de la gencive sans cicatrice ni fibrose. Ces propriétés font des FGs un candidat potentiel pour une approche de thérapie cellulaire de l’AAA. Les FGs murins cultivés in vitro produisent une grande quantité de TIMP 1, un inhibiteur des MMPs. Lorsque les FGs sont implantés autour de l’aorte, ils survivent, prolifèrent et s’organisent en une épaisse couche cellulaire au niveau de l’adventice. In vivo, les FGs produisent au niveau de la paroi aortique de l’IL-10, du TGF-β, du collagène et du TIMP-1. Parallèlement les FGs inhibent l’activité protéolytique de la MMP-9. Dans un modèle d’AAA induit par l’élastase, les FGs inhibent la dégradation de l’élastine, l’infiltration macrophagique et lymphocytaire ainsi que la croissance des AAA. L’invalidation génétique de TIMP-1 dans les FG abolit leur effet protecteur sur le remodelage aortique et la formation des AAA. Dans un second modèle d’AAA induit par l’infusion d’angiotensine II et l’injection d’anticorps neutralisant anti-TGF-β, les FGs limitent localement le développement de la maladie vasculaire et préviennent la rupture de l’aorte abdominale. L’ensemble de ces données expérimentales suggère qu’une approche de thérapie cellulaire utilisant les FG pourrait permettre de ralentir la croissance de l’AAA et in fine sa ruptureAbdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinases-mediated extracellular matrix destruction. Depiste improvement in the understanding of the pathophysiology of the aortic aneurysm disease, no pharmacological treatment is available to limit dilatation and/or rupture. In the study reported here, we tested whether periadventitial allograft of GF prevented abdominal aortic aneurysmal growth and rupture in mice and investigated the mechanisms of vascular protection. In vitro, mouse GF proliferated and produced large amounts of anti-inflammatory cytokines and Timp-1, an inhibitor of metalloproteinases. When layed down in the periadventitial abdominal aorta, we documented that GF survived in vivo, proliferated and organized as a thick layer. Furthermore, GF locally produced Il-10, TGF-β and Timp-1. In an elastase-induced AAA, GF prevented both macrophage and lymphocyte infiltration, elastin degradation and aneurysm growth. Specific invalidation of Timp-1 in GF abolished the beneficial effect of cell therapy. In an Angiotensin II/anti-TGF-β model of AAA, GF cell therapy limited AAA development and prevented abdominal rupture. Gingival fibroblast is a promising cell therapy approach to inhibit aneurysmal progression and rupture through the local production of Timp-1
6
Soheili-Majd, Esmat. "Etudes des mécanismes de la cytotoxicité des biomatériaux dentaires sur les fibroblastes pulpaires et gingivaux humains : effets des anti-oxydants." Paris 5, 2004. http://www.theses.fr/2004PA05M122.
Abstract:
La toxicité de dix biomatériaux d'obturation dentaire, non amalgame est étudiée et comparée sur les fibroblastes gingivaux et pulpaires humains : Ces biomatériaux sont toxiques in vitro. Les céments verre ionomères sont plus toxiques que les composites. Leur éluats diminuent le taux de glutathion intracellulaire (GSH). Certains antioxydants comme la NAC, le glutathion-ester et Trolox inhibent la déplétion du GSH et protègent contre ces effets toxiques. La vitamine C augmente la déplétion du GSH et la toxicité des éluats à base de CVI contenant des traces de fer. Le stress oxydatif pourrait constituer un des mécanismes essentiels de la toxicité des biomatériaux dentaires.
7
Kut-Lasserre, Christelle. "Effet protecteur des insaponifiables d'huile d'avocat et de soja sur la matrice conjonctive gingivale et sur leur capacite a remodeler cette matrice par les fibroblastes gingivaux humains en culture : etude ex vivo et in vitro." Paris 5, 1999. http://www.theses.fr/1999PA05M083.
8
Papa, Steve. "Evaluation de l'adhérence gingivale et du potentiel antibactérien de surfaces structurées par laser femtoseconde pour l'implantologie orale." Electronic Thesis or Diss., Saint-Etienne, 2023. http://www.theses.fr/2023STET0063.
Abstract:
Cette thèse traite des problèmes d'infections des implants dentaires, telles que la péri-implantite, causées par l'adhérence de bactéries parodontopathogènes. Elle explore l'utilisation de la texturation laser femtoseconde (fs-L) pour améliorer les propriétés des surfaces d'implants en alliage de titane (Ti6Al4V).Le projet, financé par l'ANR et mené en collaboration avec divers laboratoires, a utilisé des techniques de caractérisation avancées pour analyser les surfaces texturées et évaluer leur efficacité en conditions biologiques. Les résultats montrent que la texturation par fs-L permet de créer des structures de surface périodiques (LIPSS) micro et nanométriques, modifiant la surface de contact et par conséquent l’adhérence cellulaire et bactérienne. Les surfaces ainsi texturées ont démontré une réduction significative de l'adhérence des bactéries parodontopathogènes, telles que Porphyromonas gingivalis, réduisant ainsi potentiellement les risques de péri-implantite.Les études in vitro ont également confirmé une meilleure adhérence des fibroblastes gingivaux sur les surfaces texturées, ce qui pourrait réduire les risques d’infiltration bactérienne et ainsi améliorer la stabilité et l’intégration des implants.En conclusion, la texturation laser femtoseconde des surfaces d'implants dentaires est prometteuse pour créer des implants plus durables et doublement fonctionnalisés, améliorant l'adhérence cellulaire et possédant des propriétés antibactériennes accrues. Ces avancées ouvrent la voie à des implants répondant mieux aux défis cliniques actuels tout en contribuant à la lutte contre l'antibiorésistance. Des études supplémentaires plus proches d'une situation clinique sont prévues pour valider ces résultats et explorer les interactions entre les topographies fs-L et les réponses biologiques des tissus environnantsThis thesis addresses issues related to infections of dental implants, such as peri-implantitis, caused by the adhesion of periodontopathogenic bacteria. It explores the use of femtosecond laser (fs-L) texturing to enhance the properties of titanium alloy (Ti6Al4V) implant surfaces.The project, funded by the ANR and conducted in collaboration with various laboratories, employed advanced characterization techniques to analyze textured surfaces and evaluate their effectiveness under biological conditions. The results demonstrate that fs-L texturing can create micro and nanometric periodic surface structures (LIPSS), altering the contact surface and thus cellular and bacterial adhesion. Textured surfaces showed a significant reduction in the adhesion of periodontopathogenic bacteria, such as Porphyromonas gingivalis, potentially reducing the risks of peri-implantitis.In vitro studies also confirmed better adhesion of gingival fibroblasts to textured surfaces, which could reduce the risk of bacterial infiltration and thus improve implant stability and integration.In conclusion, femtosecond laser texturing of dental implant surfaces holds promise for creating more durable and dual-functionalized implants, enhancing cellular adhesion, and possessing increased antibacterial properties. These advancements pave the way for implants better suited to current clinical challenges while contributing to the fight against antibiotic resistance. Further studies closer to clinical settings are planned to validate these results and explore the interactions between fs-L topographies and the biological responses of surrounding tissues
9
Azevedo, Fabiola Pontes. "Avaliação comparativa do comportamento adaptativo de fibroblastos humanos cultivados de mucosa palatina não marginal e de enxerto gengival em área marginal." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-05062013-093746/.
Abstract:
Enxertos gengivais livres são importantes para garantir condições necessárias para o estabelecimento da homeostasia do periodonto de proteção. O processo de inflamação não ocorre por igual em todos os tecidos conjuntivos do organismo e os fibroblastos têm a capacidade de reagir a estímulos agressivos por meio de liberação de diversas citocinas, que desempenham importante função na formação do infiltrado inflamatório. Até o presente trabalho, não há relatos na literatura acerca da comparação do comportamento dos fibroblastos que compõem a mucosa palatina não marginal e dos fibroblastos provenientes de enxerto gengival livre (EGL) marginal em resistir aos estímulos agressores que ocorrem na doença periodontal. Dessa forma, a proposta do presente trabalho foi investigar se os fibroblastos da mucosa palatina não marginal mudariam seu perfil de secreção de citocinas quando enxertados na margem gengival. Foram coletadas biópsias da mucosa palatina no momento da cirurgia de EGL (período inicial) e após 4 meses (período final) no momento da cirurgia para recobrimento radicular. Os fibroblastos foram cultivados e estimulados com LPS de Porphyromonas gingivalis (Pg) e de Escherichia coli (Ec) por 24h e 48h para avaliação comparativa da expressão de citocinas e mediadores do reparo tecidual, como: IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF e CXCL16. As citocinas foram quantificadas no sobrenadante das células por meio de ensaio imunoenzimático (ELISA). Para a citocina IL-6, os fibroblastos da mucosa palatina não marginal mantiveram o mesmo perfil de secreção quando enxertados na área gengival marginal; para MIP-1α a secreção se mostrou aumentada de forma estatisticamente significativa pelos fibroblastos obtidos do enxerto gengival marginal após 48h de estímulo por Pg em comparação com os fibroblastos da área palatina não marginal; a secreção de IL-8 pelos fibroblastos da mucosa palatina não marginal foi maior em resposta ao desafio por LPS de Pg e os fibroblastos obtidos do enxerto gengival marginal exibiram secreção até mesmo sem o estímulo de LPS; apenas os fibroblastos do enxerto gengival marginal apresentaram secreção de TGF-β, mesmo na ausência de estímulo por LPS; a secreção de VEGF e CXCL16 não foi detectada pelos fibroblastos analisados. Conclui-se que os fibroblastos provenientes de uma mucosa palatina não marginal parecem se adaptar às condições locais quando enxertados na área gengival marginal, oferecendo evidência de sua participação efetiva na produção de mediadores inflamatórios importantes para o processo de homeostasia do periodonto marginal.Free gingival grafts are important to ensure conditions for the establishment of homeostasis of the periodontal soft tissues. The process of inflammation does not occur the same way in all connective tissues and fibroblasts have the ability to respond to aggressive stimuli through the release of various cytokines, which play an important role in the inflammatory infiltrate formation. In literature, there are no studies comparing the behavior of fibroblasts from palatal mucosa (not marginal) and fibroblasts from marginal free gingival graft (FGG) regarding their resistance towards periodontal disease aggressive stimuli. Thus, the purpose of this study was to investigate whether fibroblasts from the palatal mucosa behave differently when grafted to the gingival margin considering their mechanism of cytokine secretion. Biopsies from the palatal mucosa were collected at the time of FGG surgery (initial period) and after 4 months (final period) when surgery for root coverage was performed. The fibroblasts were cultured and stimulated with LPS of Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) for 24 and 48 hours in order to make a comparative evaluation of cytokines and mediators of tissue repair expression, such as IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF and CXCL16. Cytokines were measured in the cell supernatant by enzyme immunoassay (ELISA). For cytokine IL- 6, fibroblasts from palatal mucosa maintained the same secretion pattern when grafted to the gingival margin; for MIP-1α the secretion was significantly increased by fibroblasts from the marginal gingival graft after 48 hours of stimulation with Pg when compared to palatal mucosa fibroblasts; IL-8 secretion by palatal mucosa fibroblasts did not increase in response to Pg LPS challenge and fibroblasts from marginal gingival graft showed secretion even without the stimulus of LPS; only fibroblasts from marginal gingival graft showed secretion of TGF-β, even in the absence of LPS stimulation; VEGF and CXCL16 secretion by fibroblasts was not detected. It was concluded that fibroblasts from palatal mucosa seem to adapt to local conditions when grafted to the gingival margin area, providing evidence of its effective participation in the homeostasis of marginal periodontium through the production of important inflammatory mediators.
10
Kreidly, Mariam. "IL-34 Expression in Gingival Fibroblasts, Gingival Crevicular Fluid and Gingival Tissue." Thesis, Umeå universitet, Tandläkarutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-97861.
Abstract:
IL-34 is a protein associated with bone degenerative diseases but the role in periodontal disease is unknown. The aim of this study was to assess the expression of IL-34 in primary human gingival fibroblasts (GF) and investigate if the expression is regulated by the pro-inflammatory cytokines interleukin-1 (IL-1β) and tumor necrosis factor α(TNF-α). We also investigated if IL-34 is detectible in gingival crevicular fluid (GCF) in healthy, gingivitis and periodontitis sites. Furthermore, we examined if healthy and inflamed gingival tissue contains IL-34. GF were stimulated by IL-1β300 pg/ml and TNF-α10 ng/ml. IL-34 mRNA was measured by quantitative real-time PCR (qPCR). GCF was collected from 11 healthy, 10 gingivitis, and 21 periodontitis gingival crevices. IL-34 protein was quantified using enzyme-linked immunoabsorbent assays (ELISA). Healthy and inflamed gingival tissue biopsies were collected and examined using immunohistochemistry (IHC). IL-34 mRNA was expressed in GF and the expression was enhanced 12x fold-change versus control by TNF-α10 ng/ml and 4x fold-change versus control by IL-1β300 pg/ml. IL-34 was also present in GCF but no significant difference in IL-34 protein was detected between the healthy, gingivitis, and periodontitis groups. Healthy and inflamed gingival tissue showed equal amounts of IL-34 protein in the epithelium while sub-epithelially the inflamed tissue showed higher levels of IL-34 protein. Pro-inflammatory cytokines stimulate IL-34 mRNA expression in GF. IL-34 protein is present in GCF and gingival tissue which demands further investigation about the eventual role of IL-34 in the pathogenesis of periodontitis.
11
Cherifi, Hafida. "Le fibroblaste gingival : une cellule à potentiel thérapeutique pour l’anévrisme aortique." Thesis, Paris Est, 2014. http://www.theses.fr/2014PEST0058.
Abstract:
Introduction.Le fibroblaste gingival (FG) est la cellule majoritaire de la gencive. Cette dernière fait face constamment aux agressions physico-chimiques, infectieuses et thermiques. L'une des caractéristiques de la gencive est sa réparation quasi-parfaite suite à une lésion ponctuelle. Ce n'est pas le cas pour d'autres tissus comme la paroi aortique. L'anévrisme aortique (AA) est un affaiblissement de la paroi aortique provoqué par une sécrétion exhaustive de métalloprotéases (MMPs) et en particulier de MMP-9. Il en résulte une dilatation de l'artère. Dans un modèle d'anévrisme de lapin, Durand et al (2012) avait montré que le FG pouvait ralentir, voire réparer un anévrisme. Dans notre étude, nous avons mis en place un modèle de coculture FG/AA d'origine humaine.Chez l'homme, la localisation de la pathologie peut être au niveau abdominal (Anévrisme Aortique Abdominale : AAA) ou thoracique (Anévrisme Aortique Thoracique : AAT). Etant donné que leur étiologie sont différentes, nous avons souhaité savoir s'il existait des différences selon les lésions. Cela nous permettrait en effet de mieux appréhender la prise en charge. Nous avons réalisé une étude comparative histo et physiopathologique entre les AAA et AAT. L'une des différences soulevée, est la présence d'un facteur infectieux au niveau des AAA. C'est un élément à prendre en compte pour une thérapie cellulaire et ainsi nous avons mis en culture des FG en présence de LPS, une endotoxine bactérienne.De plus pour approfondir notre travail sur l'utilisation du FG dans la thérapie cellulaire, nous avons initié une étude sur la plasticité de la sous-population souche des FG en étudiant, notamment leur orientation en cellules vasculaires (cellules endothéliales).Résultats/discussionLe FG, grâce à sa secrétion de TIMP-1, contribue à l'inhibition de la MMP-9 anévrismale. La sécrétion de MMP-9 est plus importante dans les lésions avec athérome (AAA) que celles sans athérome (AAT dans notre étude). Ceci est en corrélation avec la dégradation qui est plus importante dans les AAA que dans les AAT. La MMP-9 est une protéine sécrétée entre autre par les cellules inflammatoires. Une inflammation est présente dans les AAA et pas dans les lésions thoraciques. Ceci pourrait expliquer la différence de sécrétion de MMP-9 et donc de dégradation. Concernant l'origine de cette inflammation, nous avons recherché une cause infectieuse. Porphyromonas gingivalis (Pg) qui est une bactérie importante dans le développement de la parodontite (maladie inflammatoire des tissus de soutien de la dent) a été détectée dans les AAA. Une relation pathologique existerait entre la parodontite et l'AAA mais l'étude devrait être plus poussée pour connaître le mécanisme physiopathologique de ce phénomène. Toutefois, en ce qui concerne la thérapie cellulaire, le LPS qui est une endotoxine du Pg, n'affecte pas la capacité du FG à secréter du TIMP-1.En plus de la possibilité du FG à neutraliser la MMP-9 anévrismale, nous avons souhaité savoir si le FG avait des compétences de différentiation en cellule vasculaire. Un début d'exploration de la plasticité cellulaire de la souche multipotente de FG en cellule endothéliale, donnent des résultats préliminaires encourageants.Conclusion. Le FG pourrait être une cellule prometteuse pour une thérapie cellulaire de l'anévrisme aortique mais des explorations plus poussées sont encore nécessaires pour une telle applicationIntroductionGingival fibroblast (GF) is the main cell in gingiva which is constantly facing infectious, thermal and physico-chemical attacks. When a lesion occurs, the repair of gingiva is almost perfect. It is not the case for other tissues as the aortic wall. The aortic aneurysm (AA) is a pathologic expansion of aorta due to a weakening of the wall with an exhaustive secretion of metalloproteinases (MMPs) and particularly of MMP-9. In an aneurysm rabbit model, Durand and al (2012) have showed that GF could slow down or repair the aneurysm. In our study, we have established a co-culture model of human GF and human AA.For human, the location of the aortic disease may be at abdominal level (Abdominal Aortic Aneurysm: AAA) and thoracic level (Thoracic Aortic Aneurysm: TAA). Since the aetiologies are different, we wondered if histo and physiopathologic differences would existe between the both. It is impotant to know that for better supporting the disease. One of the difference between AAA and TAA is the presence of an infectious factor in AAA. This is an element to consider for cell therapy, so we studied the behavior of GF in presence of an endotoxin, the LPS.In addition, to further our work on the use of GF in cell therapy, we have initiated a study of the plasticity of the GF multipotente subpopulation including the differentiation into vascular cells (endothelial cell in particular).Results/DiscussionThanks to its TIMP-1 secretion, GF could contribute to the inhibition of MMP-9 activity in aneurysm. The secretion of MMP-9 in AA with atheroma (AAA) is highter than in TAA (without atheroma in our study). It is correlated to the degradation of AAA which is more important than the degradation of TAA. Inflammatory cells may secrete MMP-9. Inflammation is present in AAA and not in TAA. This, could explain the highter secretion of MMP-9 in abdominal lesion and also the degradation which is more important in AAA than in TAA. As for the origin of this inflammation, we researched an infectious factor. We isolated Porphyromonas gingivalis (Pg) in AAA, which might trigger or aggravate inflammation. This is an important bacterium in the development of periodontitis (inflammatory disease of the tissues supporting the tooth). A pathological relationship may exist between periodontitis and the AAA. The study should be further to know the pathophysiology of AAA related to Pg. But as regards the cell therapy, LPS, which is an endotoxin of Pg would not affect the secretion of TIMP-1 by the GF.In addition to its abilities to inhibate MMP-9 in aneurysm, we wondered if GF would be able to differentiate into vascular cell. An early exploration of GF multipotent subpopulation plasticity reveals a possible opportunity to go further in a the cell therapy.Conclusion.GF might be a promising cell for treating aortic aneurysm but further explorations are still necessary for its application
12
Rodrigues, Annelissa Zorzeto. "Avaliação in vitro do cultivo de fibroblastos gengivais humanos em matriz dérmica acelular." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-30062008-150532/.
Abstract:
A matriz démica acelular, MDA, figura dentre os biomateriais que têm por objetivo restaurar defeitos mucogengivais. A correção de defeitos mucogengivais a partir de constituintes autógenos são os procedimentos mais comumente usados, no entanto, em decorrência da quantidade insuficiente de tecido doador, esses procedimentos se tornam limitados. Diante disso, o objetivo desse estudo foi avaliar, in vitro, diferentes aspectos relacionados ao cultivo prévio de fibroblastos gengivais humanos em MDA. Fibroblastos gengivais humanos foram cultivados pela técnica do explante a partir de amostras de tecido gengival queratinizado removido de três pacientes saudáveis. A MDA foi cultivada com esses fibroblastos por períodos de 14 e 21 dias para posterior análise dos eventos de: adesão celular, proliferação e viabilidade. Os resultados mostraram que em 7 dias, os fibroblastos estavam aderidos, espraiados e dispersos sobre a superfície externa da MDA, em 14 dias formavam monocamada de células de morfologia alongada e quiescentes (Ki-67 negativos) em sua maioria, sendo apenas ocasionalmente observadas no interior da MDA. Em 21 dias a monocamada exibia menor densidade celular. Os resultados sugerem que o cultivo de fibroblastos em MDA em períodos de 14 dias permite boas condições de adesão e espraiamento das células sobre a matriz, porém, a alta densidade de fibras colágenas parece ser um fator limitante à migração celular.Acellular Dermal Matrix, ADM, is a biomaterial that has been used in periodontal procedures to treat mucogingival defects. Mucogingival defects can be corrected by autogenous grafts that are the most common procedure used in periodontology, however, because of the limited source of donor\'s tissue this procedure became limited. The aim of this investigation was to verify, in vitro, different aspects related to human gingival fibroblasts seeding on to the ADM. Human gingival fibroblasts were established from explant cultures from the connective tissue of keratinized gingiva collected from three healthy patients. ADM was seeded with gingival fibroblasts for 14 and 21 days, and then cell adherence, proliferation and viability were analyzed. Results revealed that, at day 7, fibroblasts were adherent and spreading on the ADM surface, and were unevenly distributed, forming a discontinuous single cell layer, at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. The results suggests that fibroblasts seeding on the ADM for 14 days can allow good conditions for cell adhesion and spread on the matrix, however, because of the high collagen fiber bundle density cell, migration inside the matrix was limited.
13
Loison-Robert, Ludwig. "Cellule souche gingivale : origine et multipotence." Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC0083/document.
Abstract:
La gencive correspond à un modèle de régénération naturelle grâce notamment à sa capacité de cicatrisation « ad integrum ». Ce phénomène est permis par sa composition en fibroblastes gingivaux. Ces cellules, composante cellulaire principale du tissu conjonctif gingival, sont au cœur de la régulation des réponses inflammatoires et de la cicatrisation. Ce tissu contient, comme d’autres tissus mésenchymateux, des cellules souches ; qui expliquent en partie ces capacités de régénération. De plus, comme le tissu gingival est abondant et facilement accessible, l’utilisation de ces cellules souches pourraient être d’un intérêt prometteur en thérapie cellulaire ou pour de la modélisation in vitro. Au cours de cette thèse, nous avons pu montrer que les Cellules Souches dérivées de la Gencive Humaine (CSGH) possèdent des propriétés communes avec les cellules souches adultes dérivées des crêtes neurales. Ces cellules peuvent être qualifiées de « souche » par leur capacité d’auto-renouvèlement, d’adhésion au plastique et de multipotence. Premièrement, nous avons montré que la méthode ainsi que les produits de culture utilisés pour l’isolation des fibroblastes gingivaux in vitro à partir de biopsies de gencive avait une influence sur les cellules obtenues. Dans un second temps, une analyse clonale in vitro de populations de fibroblastes gingivaux a permis de montrer que les fibroblastes gingivaux sont composés de sous-populations qui expriment des marqueurs spécifiques des cellules souches et des crêtes neurales. Outre leur origine embryologique, l’étude de leur multipotence a aussi été caractérisée après expansion et en fonction des additifs utilisés. Pour finir, deux exemples d’utilisation de ces cellules comme modèle d’étude de la biocompatibilité de biomatériaux in vitro ont été développés; imitant la muqueuse buccale ainsi que les réactions dentaires (réparatrices et réactionnaire)Gingiva is a natural regeneration model thanks to its "ad integrum" healing capability. Gingival fibroblasts are the main actors of this property. These cells, the main cellular component of the gingival connective tissue, regulate the inflammatory responses and healing process. This tissue contains, like many others, mesenchymal stem cells; which also partly explain these regenerative abilities. Moreover, as the gingiva is abundant and easily accessible, the use of these stem cells may interest cell therapy or in vitro model tissues responses. In this work, we demonstrated that Stem Cells Derived from Human Gingiva (SCHG) have common properties with neural crest adult stem cells. These cells can be called "stem cells" for their ability to self-renew, adhere to plastic and to differentiate. First, we have shown that the method and the culture products used for isolation of gingival fibroblasts from gingival biopsy had an influence on the obtained cells. Secondly, an analysis of in vitro clonal populations of gingival fibroblasts has shown that gingival fibroblasts are composed of subpopulations that express specific markers of stem cells and neural crests. In addition to their embryological origin, the study of their multipotency was also characterized after expansion and depending on the used additives. Finally, two examples of using these cells and dental pulp stem cells as a model to study the in vitro biocompatibility of biomaterials have been developed, mimicking oral mucosa or dentin reactions (reparative or reactional)
14
Sobral, Lays Martin. "Participação de Smad7 e CTGF na transdiferenciação de miofibroblastos gengivais e análise da influência dos miofibroblastos na proliferação e invasão de carcinomas espinocelulares orais." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288721.
Abstract:
Orientador: Ricardo Della ColettaTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Miofibroblastos são células mesenquimais caracterizadas pela expressão da isoforma ? da actina de músculo liso (?-SMA) e pela secreção de proteínas da matriz extracelular, fatores de crescimento e proteases. Estas células desempenham um papel importante na reparação de feridas e em processos patológicos, incluindo fibroses e cânceres. Os objetivos deste estudo foram 1) analisar o papel do fator de crescimento de tecido conjuntivo bem como o efeito da superexpressão de Smad7 na transdiferenciação de miofibroblastos gengivais induzida pelo fator de crescimento transformante-?1 (TGF-?1), 2) isolar e caracterizar linhagens celulares de miofibroblastos do estroma de carcinomas espinocelulares (CEC) orais e comparar o potencial proliferativo e produção de metaloproteinases de matriz (MMP) com linhagens celulares de fibroblastos do estroma de CEC orais, e 3) analisar a influência de miofibroblastos na modulação da proliferação e invasão de linhagens celulares de CEC oral. Nossos resultados demonstraram que o tratamento com TGF-?1 induziu simultaneamente a expressão de ?-SMA e CTGF e a neutralização de CTGF com RNA de interferência (siRNA) bloqueou o efeito de TGF-?1 na indução da transdiferenciação de células de gengiva normal em miofibroblastos. A superexpressão de Smad7 em células de GN inibiu a cascata de ativação de TGF-?1, caracterizada pela fosforilação de Smad2 e expressão de ?-SMA, CTGF e colágeno tipo I. Similarmente, miofibroblastos isolados do tecido gengival de fibromatose gengival hereditária (FGH) superexpressando Smad7 demonstraram níveis reduzidos de ?-SMA e pSmad2, além de baixos níveis de expressão de CTGF e colágeno tipo I. Três linhagens celulares de miofibroblastos foram isoladas do estroma de CEC de língua e caracterizadas pela expressão de ?-SMA e por níveis elevados de produção de colágeno tipo I. Embora o potencial proliferativo dos clones de fibroblastos e miofibroblastos tenham sido semelhantes, as produções de MMP-1, -2, -9 e -13 foram significantemente maiores em miofibroblastos. Finalmente, nós demonstramos que miofibroblastos do estroma tumoral produzem níveis elevados de alguns fatores de crescimento comparado com fibroblastos, incluindo ativina A. Meios de cultura condicionados por miofibroblastos contendo ativina A significantemente induziu a proliferação de linhagens celulares de CEC oral e uma maior progressão tumoral in vivo, enquanto que o bloqueio de ativina A por siRNA diminuiu significantemente a proliferação das células de CEC oral. In vitro, miofibroblastos induziram a invasão de linhagens celulares de CEC oral, o qual foi acompanhado por uma indução na produção de MMPs, e in vivo uma significante correlação entre presença de miofibroblastos e atividades de MMP-2 e MMP-9 foi observada. O bloqueio da síntese de ativina A por siRNA em miofibroblastos não alterou a capacidade de indução da invasão e síntese de MMPs. Os resultados deste estudo demonstram 1) que Smad7 bloqueia a transdiferenciação de miofibroblastos gengivais por meio da inibição da fosforilação de Smad2 e da transcrição de CTGF, 2) que miofibroblastos no estroma de CEC orais podem contribuir para um fenótipo mais invasivo via secreção de elevados níveis de MMPs e 3) que produtos de síntese dos miofibroblastos induzem a proliferação e invasão das células de CEC oral e os estímulos proliferativos são controlados pela produção de ativina A.
Abstract: Myofibroblasts are mesenchymal cells, characterized by the specific isoform ? of the smooth muscle actin (?-SMA) expression and the extracellular matrix proteins, growth factors and proteases secretion. These cells play a central role on wound healings and in pathologic process, including fibrosis and cancers. The aims of this study were 1) analyze the connective tissue growth factor (CTGF) role and the superexpression of Smad7 effect on TGF-?1-induced gingival myofibroblasts transdifferentiation, 2) isolate and characterize myofibroblast cell lines from oral squamous cell carcinomas stroma (OSCC) and compare the proliferative potential and matrix metalloproteinases (MMP) production with fibroblast cell lines from OSCCs stroma, and 3) analyze the myofibroblasts influence on the modulation of OSCC cell lines proliferation and invasion. Our results demonstrated that the TGF-?1 treatment induced simultaneously the ?-SMA and CTGF expression and the CTGF neutralization using the small interference RNA (siRNA) blocked the TGF-?1-induced gingival myofibroblasts transdifferentiation. Smad 7 superexpression in normal gingival cells (NG) inhibit the TGF-?1 cascade activation, characterized by the Smad2 phosphorilation and ?-SMA, CTGF and type I collagen expression. Similarly, hereditary gingival fibromatosis (HGF) myofibroblasts superexpressing Smad 7, demonstrated reduced levels of ?-SMA and phospho-Smad2, and low expression levels of CTGF and type I collagen. Three myofibroblast cell lines were isolated from tongue OSCC stroma and characterized by the ?-SMA expression and high levels of type I collagen. Although the proliferative potential of fibroblast and myofibroblast clones has been similar, the MMP-1, -2, -9 and -13 were significantly higher in myofibroblasts. Finally, we demonstrated that tumor stroma myofibroblasts produce high levels of some growth factors compared with fibroblasts, including activin A. Myofibroblasts conditioned medium containing activin A induce significantly the OSCC cell lines proliferation and a tumor progression in vivo, while the activin A dowregulation by siRNA significantly decreased the OSCC cells proliferation. In vitro, myofibroblasts induced OSCC cells invasion, accompanied by an induction of MMPs production, and in vivo was observed a significant correlation between the myofibroblasts presence and the MMP-2 and MMP-9 activity. The myofibroblasts dowregulation of activin A by siRNA did not affect the induction of invasion and MMPs synthesis. The results of this study demonstrate that 1) Smad 7 blockage the gingival myofibroblasts transdifferantiation through the inhibition of Smad 2 phosphorilation and CTGF transcription, 2) myofibroblasts on the OSCCs stroma can contribute to a more invasive phenotype via elevated levels of MMPs secretion, 3) myofibroblasts released products induce an OSCC cells proliferation and invasion and the proliferative stimulus are controlled by the activin A production.
Doutorado
Estomatologia
Doutor em Estomatopatologia
15
Yucel-Lindberg, Tülay. "Regulation of prostaglandin E₂ synthesis in human gingival fibroblasts /." Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3734-6/.
16
Vičiūnaitė, Neringa. "Skirtingų titano implantų paviršiaus modifikavimo strategijų poveikis dantenų fibroblastų savybėms." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120726_143425-36935.
Abstract:
Baigiamajame darbe įvertintas skirtingų titano implantų paviršiaus modifikavimo
strategijų poveikis pirminių dantenų fibroblastų savybėms – adhezijai, proliferacijai ir
diferenciacijai. Tyrimuose naudoti titano mėginiai, kurių paviršiaus šiurkštumas pakeistas
fizikiniais metodais – smėliasrove ir lazeriu. Iš gingivektomijos metu paimto ţmogaus
dantenų subepitelinio audinio buvo išskirtos ir charakterizuotos dantenų fibroblastų ląstelės.
Tiriant šių ląstelių adheziją ant modifikuotų titano mėginių paviršių nustatyta, kad
ankstyvuoju laikotarpiu dantenų fibloblastų adhezija buvo panaši ant visų tirtų įvairiai
modifikuotų titano mėginių paviršių, tačiau vėliau efektyviausias ląstelių prikibimui
paviršius buvo lazeriu suformuotos grotelės. Siekiant pagerinti modifikuotų titano paviršių
adhezines savybes, jie papildomai buvo padengti tarpląstelinio uţpildo baltymais – kolagenu
ir lamininu, įvertintas tokio padengimo poveikis ląstelių prikibimui ir augimui. Analizuojant
mechanizmus, reguliuojančius ląstelių adhezijos ant modifikuotų paviršių procesus, buvo
tirta FAK ir Akt kinazių raiška ir aktyvinimas. Vertinant karkasų paviršiaus topografijos
poveikį ląstelių diferenciacijai, buvo palygintas osteogeninės diferenciacijos laipsnis
ląstelėse augintose ant įvairiai modifikuotų titano mėginių paviršių.
Darbą sudaro 6 dalys: įvadas, literatūros apţvalga, medţiagos ir metodai, rezultatai ir
jų aptarimas, išvados, literatūros sąrašas.
Darbo apimtis – p. teksto be priedų, 24 pav., 2 lent... [toliau žr. visą tekstą]The influence of different modification strategies of titanium implant surfaces on gingival fibroblasts properties - adhesion, proliferation, and differentiation was studies in this final master thesis. Different titanium surface roughness modifications using physical methods such as sand-blasting as well as laser irradiation were developed. Gingival fibroblasts derived from human gingival subepithelial tissues obtained during gingivectomy were isolated and characterized. The data suggested that the initial adhesion between tested cells and various modified titanium surfaces was similar, but the most efficient surface for subsequent cell attachment was laser-ablated holey arranged in grid-like structures. Additionally, in order to improve the modified titanium surface adhesion properties, these surfaces were coated by extracellular matrix proteins - collagen and laminin. The coating influence on the cell growth and adhesion was evaluated. To analyze the mechanisms regulating cell adhesion processes on the modified surfaces, FAK and Akt kinase expression as well as activation were studied. In order to evaluate the effect of surface topography on cell differentiation, the level of osteogenic differentiation was compared. Structure: introduction, literature review, materials and methods, results and discussion, conclusions, references.
17
Williams, Rachel Claire. "Leptin regulation of inflammatory responses in human gingival fibroblasts." Thesis, University of Newcastle upon Tyne, 2015. http://hdl.handle.net/10443/2976.
Abstract:
Obesity and type 2 diabetes mellitus (T2DM) are positively associated with the destructive, chronic inflammatory disease periodontitis. The adipokine leptin is elevated in obesity and T2DM, and promotes inflammatory responses. Gingival fibroblasts are implicated in the pathogenesis of periodontitis because inflammatory stimuli can drive these cells towards destructive, inflammatory responses. The aim of this study was to identify whether leptin promotes inflammatory responses in gingival fibroblasts, focussing on the extracellular matrix-degrading matrix metalloproteinases (MMPs), and their inhibitors (TIMPs). Primary human gingival fibroblasts (hGFs) were isolated from gingival tissue and cultured in vitro. RT-PCR, ELISA and flow cytometry were used to assess MMP, TIMP and TLR expression; hGF signalling (±chemical pathway inhibitors) was assessed by Western blotting. hGFs constitutively expressed numerous MMPs and TIMPs. Leptin increased the expression of MMP-1, MMP-3, MMP-8 and MMP-14, but not TIMPs, in hGFs. Leptin and interleukin-1 or the TLR2 agonist pam2CSK4 synergistically increased MMP-1 and MMP-3 production by hGFs; in contrast, leptin and Escherichia coli LPS regulated MMP production in a donor-dependent manner. TLR4 was detected on the surface of hGFs from all donors tested, suggesting that differential responses to E. coli LPS were not due to absent cell surface TLR4. Overall, these results suggest that leptin promotes an ECM-degrading hGF response, which is further enhanced under inflammatory conditions. Leptin activated multiple intracellular signalling pathways, but the MAPK pathway and ERK in particular, regulated leptin-stimulated MMP-1 expression in hGFs. Genome-wide expression profiling revealed that leptin enhanced the expression of genes in hGFs whose products function in inflammation; similarly, leptin enhanced the inflammatory gene expression profile induced by IL-1 in hGFs. Leptin+interleukin-1 did not promote collagen degradation in human gingival connective tissue explants. In conclusion, leptin enhances wide-ranging inflammatory responses in hGFs in a context-dependent manner. This response could be a mechanistic link between obesity, T2DM and periodontitis.
18
McKnight, Holly A. "PROTEOMIC ANALYSIS OF MEMBRANE BOUND AND ASSOCIATED PROTEINS OF HUMAN GINGIVAL FIBROBLASTS AND PERIODONTAL LIGAMENT FIBROBLASTS." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338325450.
19
Pfeiffer, Friederike. "Untersuchung des Adhäsionsverhaltens von Gingiva-Fibroblasten auf mikrostrukturierten Titanoberflächen." [S.l. : s.n.], 2004. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB11195193.
20
Ferré, François. "Isolation et caractérisation des cellules souches gingivales : étude de leur potentiel multipotent." Phd thesis, Université René Descartes - Paris V, 2013. http://tel.archives-ouvertes.fr/tel-01017172.
Abstract:
Les capacités de cicatrisation de la gencive en font un modèle de régénération tissulaire naturelle. Ces capacités sont liées en grande partie à l'activité des fibroblastes. Composante cellulaire principale du tissu conjonctif gingival, ils sont au cœur de la régulation des réponses inflammatoires et des processus de cicatrisation. Nous avons supposé que ce tissu pouvait contenir des cellules souches, pouvant expliquer en partie, ces capacités de réparation. Au cours de cette thèse, nous avons pu mettre en évidence la présence de cellules souches mésenchymateuses aux propriétés communes avec les cellules souches adultes dérivées des crêtes neurales. Ces cellules expriment des marqueurs spécifiques des cellules souches et des crêtes neurales. Par ailleurs, elles présentent des capacités d'auto-renouvellement et de multipotence. Elles sont, en effet, capables de se différencier en adipocytes, ostéocytes et chondrocytes. Nous nous sommes plus particulièrement intéressés à la différenciation chondro/endochondrale. La culture des cellules, sous forme de sphères en suspension, a permis de mettre en évidence leurs capacités de différenciation en tissus cartilagineux et articulaires. Elles s'organisent spontanément en plusieurs types cellulaires différents, générant notamment des chondrocytes hypertrophiques et des synoviocytes selon leur localisation au sein des sphères et du milieu de culture utilisé. Le comportement de ces cellules soumises à ces conditions a permis de montrer leurs facultés à reproduire, in vitro, des processus proches de ceux retrouvés au cours du développement. Ces résultats permettent une meilleure compréhension des phénomènes de différenciation des cellules souches adultes, ouvrant ainsi de nouvelles perspectives pour des applications en thérapie cellulaire articulaire et osseuse.
21
Wallis, Jeffrey S. "Changes in gene expression in cyclosporine A treated gingival fibroblasts." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file 1.91 Mb., 84 p, 2005. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:1428256.
22
Lappin, M. J. "The role of gingival fibroblasts in the innate immune response." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546377.
23
Javaid, Mohammad. "Platelet factor 4 upregulates matrix metalloproteinase-1 production in gingival fibroblasts." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/60244.
Abstract:
Background and Objective: Periodontitis is a highly prevalent chronic inflammatory disease that causes tooth loss, morbidity and confers an increased risk for systemic disease. Tissue destruction during periodontitis is due in large part to collagen-degrading matrix metalloproteinases (MMPs) released by resident cells of the periodontium in response to pro-inflammatory cytokines. Platelets are immune-competent blood cells with a newly recognized role in chronic inflammation, however their role in the pathogenesis of periodontitis is undefined. Consequently, the objective of this study was to assess the effect of platelet factor 4 (PF4), a major platelet-derived cytokine, on MMP-1 (collagenase) expression in human gingival fibroblasts (HGFs).
Methods: HGFs were cultured in the presence or absence of recombinant PF4. Pro-MMP-1 secretion was quantified by enzyme-linked immunosorbent assay (ELISA) analysis of the cell culture supernatants. MMP-1 transcription was quantified by real-time polymerase chain reaction (qPCR). Regulation of MMP-1 production by the p44/42 MAP kinase (MAPK) signaling pathway was examined in the presence or absence of PF4.
Results: Exposure to PF4 caused a ~2-3-fold increase in MMP-1 transcription and secretion from cultured human gingival fibroblasts (HGFs). PF4 treatment also enhanced phosphorylation of p44/42 MAP kinase (MAPK), which has been previously shown to induce MMP-1 expression in fibroblasts. Blockade of p44/42 MAPK signaling with the cell-permeant inhibitors PD98059 and PD184352 abrogated PF4-induced pro-MMP-1 transcription upregulation and release from cultured HGFs.
Conclusion: We conclude that platelet factor 4 upregulates MMP-1 expression in human gingival fibroblasts in a p44/42 MAPK-dependent manner. These findings point to a previously unidentified role for platelets in the pathogenesis of periodontal diseases.Dentistry, Faculty of
Graduate
24
Palmqvist, Py. "Osteotropic cytokines : expression in human gingival fibroblasts and effects on bone." Doctoral thesis, Umeå : Umeå universitet, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-960.
25
Ouyang, Ying. "Effect of zinc on the metabolism of thiol-treated human gingival fibroblasts." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/30247.
Abstract:
The etiology of periodontal diseases is multifactorial. Cumulative in vivo and in vitro observations implicate volatile sulphur compounds (VSC), namely, hydrogen sulphide (H₂S) and methyl mercaptan (CH₃SH), produced through putrefaction of sulphur-containing proteinaceous substrates by gram negative bacteria, in the pathogenesis of the diseases. Their concentrations in the mouth air correlate with the severity of periodontal involvement. They significantly inhibit total protein, DNA and collagen synthesis, and proline transport in cultured human gingival fibroblast (HGF) systems. The inhibition on total protein synthesis is irreversible, with CH₃SH exerting a more profound inhibitory effect than H₂S. In addition, they have been found to increase the permeability of porcine sublingual mucosa to [3H]-PGE₂, [35S]-SO₄ and E. coli endotoxin thus suggesting that VSC may be capable of altering the permeability of the epithelial barrier and thereby assist the pathogens to gain access to the underlying connective tissues. However, treatment of the mucosa with 0.22% ZnCl₂ totally nullifies the effect of VSC and restores the tissue permeability to control state. The objective of this thesis is to investigate whether zinc is also capable of reversing the inhibitory effect of CH₃SH on total protein, DNA and collagen synthesis, and proline transport by cultured HGF.
While ZnCl₂ at concentration of 0.22% (1.6x10⁻² M) is protective against VSC
and without any apparent harmful effects when applied to the intact tissue, HGF cells, when in direct contact with this agent, are more sensitive to it. In order to determine the maximal zinc concentration that is not deleterious to HGF, studies of total protein synthesis, cell attachment and proliferation were conducted on cultured HGF exposed to various concentrations of ZnCl₂ ranging from 1.0x10⁻⁶M to 1.5x10⁻²M. It was found that zinc at concentrations higher than 1.0x10⁻⁴M significantly decreased the total protein synthesis by HGF over a 24-hr labeling period in DMEM devoid of L-proline but supplemented with L-[′⁴C]-proline. Fibroblast attachment was reduced by 47% and 21% when they were treated with 1.5x10⁻²M and 0.5x10⁻3 M ZnCl₂, respectively. Fibroblasts incubated in the presence of 1.5x10⁻² M and 0.5x10⁻3 M ZnCl₂ failed to proliferate over a 11-day growth period and under the light microscope were rounded in appearance. Transmission electron microscopic studies of fibroblasts treated for 24 hours with different concentrations of ZnCl₂ revealed that cells exposed to higher than 1.0x10⁻⁴M ZnCl₂ underwent pathologic changes characterized by clumping of nuclear chromatin, presence of large amount of myelin figures and overall disintegration of cytoplasmic organelles. In addition, it was found that exposure of HGF cultures for 24 hours to [⁶⁵Zn] resulted in an accumulation of zinc by fibroblasts in a concentration-dependent manner.
To assess the ability of zinc to counteract CH₃SH, control HGF cultures were
incubated in an atmosphere of 95% air / 5% CO₂ while test cultures were subject to
95% air / 5% CO₂ admixed with 15ng / ml CH₃SH with or without the presence of ZnCl₂ in the culture media. It was found that during a 24-hr L-[′⁴C]-proline pulsing period, exposure of HGF to CH₃SH resulted in a 24% reduction in total protein synthesis which
could be totally reversed by the presence of 0.5x10⁻⁴M or 1.0x10⁻⁵ M ZnCl₂ in the media. Furthermore, at these two concentrations, zinc was also found effective in reversing the CH₃SH-induced inhibition of proline transport. HPLC analysis of total protein and collagen confirmed that ZnCl₂ present at a concentration of 1.0x10⁻⁵ M in the media totally nullified the inhibitory effect of CH₃SH and restored total protein synthesis by HGF to control levels. However, at this concentration, ZnCl₂ did not reverse the effect of CH₃SH on collagen synthesis, and at non-inhibitory concentrations (below 0.5x10⁻⁴ M), it was ineffective in reversing the adverse effect of CH₃SH on DNA synthesis by cultured HGF.
In summary, zinc at non-inhibitory concentrations in HGF culture has been shown to be capable of counteracting the CH₃SH-induced suppression of total protein synthesis and proline transport by HGF, which implies that zinc may have a therapeutic role in counteracting some of the adverse effects of volatile thiols and modify their effect on tissue destruction that occurs in periodontal disease.Dentistry, Faculty of
Graduate
26
Young, Duprane Pedaci. "Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials." The Ohio State University, 1999. http://rave.ohiolink.edu/etdc/view?acc_num=osu1413475378.
27
Ishikiriama, Bella Luna Colombini. "Estudo da expressão e produção de componentes do sistema renina-angiotensina por fibroblastos de gengiva e ligamento periodontal humanos." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/25/25142/tde-08022013-084557/.
Abstract:
O Sistema Renina-angiotensina (SRA), e um sistema capaz de gerar hormonios peptideos com grande impacto na regulacao cardiovascular e na patogenese das doencas cardiovasculares. Este sistema opera, por meio das acoes da Angiotensina II, tanto em nivel sistemico (endocrino) quanto tecidual (local, paracrino/autocrino) controlando importantes funcoes, varias delas relacionadas a facilitacao da instalacao e progressao do processo inflamatorio. Por este motivo, a producao desta proteina nos tecidos pode estar relacionada a patogenese de muitas doencas, dentre elas a doenca periodontal (DP), tendo em vista seu carater infeccioso-inflamatorio e os achados da literatura que mostram que a inibicao da formacao de Ang II, diminui a perda óssea da DP em animais. Desta forma, o presente trabalho teve como objetivos: Avaliar in vitro, a) A expressao de componentes do SRA (ANGT, RENINA, ECA, ECA-2, AT1, AT2 e Mas) por fibroblastos de gengiva e ligamento periodontal humanos, por RT-qPCR; b) A producao de componentes do SRA (RENINA, ECA, ECA-2) no sobrenadante de culturas de fibroblastos de gengiva e ligamento periodontal humanos, por ELISA; c) A producao dos receptores do SRA (AT1, AT2 e Mas), nestes fibroblastos, por Imunofluorescencia e d) Se a expressao e a producao dos componentes do SRA por fibroblastos de gengiva e ligamento periodontal humanos, se alteram com a estimulacao por LPS de P. gingivalis e E. coli. Apos a coleta, os dados foram analisados com o auxilio do programa GraphPad Prism 5.0. por meio da analise de variancia a 2 criterios (ANOVA-two way) seguida do pos teste de Bonferroni, com nivel de significancia de 5% para a verificacao das possíveis diferencas. Foi detectada a expressao genica para alguns dos componentes do SRA (ANGT, RENINA, ECA, AT1) por fibroblastos tanto de gengiva quanto de ligamento periodontal. Foi detectada ainda uma expressao genica diferenciada entre fibroblastos de gengiva e ligamento periodontal para a ECA, sendo significativamente maior nos fibroblastos da gengiva. Houve imunomarcacao positiva tanto nos fibroblastos de gengiva quanto de ligamento periodontal compativel com a presenca dos receptores AT1 e Mas. Pode-se observar por fim que o contato com LPS de P. gingivalis e E. coli, na concentracao de 10 g/mL/24 h, nao alteram a expressão dos componentes do SRA. Portanto, pode-se concluir que os fibroblastos tanto de gengiva quanto de ligamento periodontal apesar de nao expressarem e produzirem todos oscomponentes do SRA necessarios para a formacao local de Ang II, poderiam contribuir, ainda que parcialmente, com outras celulas do microambiente dos tecidos periodontais para a formacao e acao locais da Ang II, e assim, para a instalacao e progressao da DP.The Renin-angiotensin system (RAS) can generate hormones that have a high-impact on cardiovascular regulation as well as in the pathogenesis of cardiovascular disease. This system acts through both systemic (endocrine) and local (paracrine/autocrine) effects of Angiotensin II, controlling important functions related to the facilitation of installation and progression of the inflammatory process. For this reason, this proteins production in tissues can be associated to the pathogenesis of many diseases, including periodontal disease (PD). In the PD setting, a infectious-inflammatory characterized disease, the literature findings shows that inhibition of the Ang II formation can decrease the bone loss in animals. In this context, the aims of the present study were: to investigate in vitro: a) the expression of RAS components (ANGT, RENIN, ECA, ECA- 2, AT1, AT2 and Mas) by human gingival and periodontal ligament fibroblasts by RT-qPCR; b) the production of RAS receptors (AT1, AT2 and Mas) by human cultured gingival and periodontal ligament fibroblasts by Immunofluorescence and d) the production of RAS components (RENIN, ECA, ECA-2) if the expression and production of RAS components by gingival and periodontal ligament fibroblasts modify under P. gingivalis and E. coli LPS stimulation. After collected, the data were analysed using GraphPad Prism 5.0, by the two way ANOVA followed by Bonferroni post test with a significance level of 5%. Gene expression was detected for some of the RAS components (ANGT, RENIN, ECA, AT1) by both gingival and periodontal ligament fibroblasts. It was detected a differential gene expression between gingival and periodontal ligament fibroblasts for ECA, being significantly higher in gingival fibroblasts. There was a stain in Immunofluorescence compatible with the production of RAS receptors (AT1 and Mas). It must be noted that the stimulation with P. gingivalis and E. coli LPS, in a concentration of 10 g/mL/24 h, did not altered the expression of RAS components. In conclusion, despite of neither gingival or periodontal ligament fibroblasts express all components of RAS, needed to local formation of Ang II, they might also contribute to the local formation and action of Ang II and in consequence, to the installation and the progression of DP.
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Domeij, Helena. "Expression and regulation of MMP-1 and MMP-3 in human gingival fibroblasts /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-544-5/.
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Sobral, Lays Martin. "Analise dos efeitos e dos mecanismos de ação do fator de crescimento transformante-'beta'1, interferon 'gama' e iclosporina A na transdiferenciação dos fibroblastos gengivais em miofibroblastos." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288728.
Abstract:
Orientador: Ricardo Della ColettaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Miofibroblastos são células especializadas que exercem funções importantes em processos fibróticos através da síntese elevada de macromoléculas da matriz extracelular, proteases e citocinas. O exato mecanismo do surgimento (transdiferenciação) dos miofibroblastos permanece desconhecido, porém inúmeros estudos sugerem que o fator de crescimento transformante-'beta'1 (TGF-'beta'1) exerça um papel importante neste processo via ativação do fator de crescimento de tecido conjuntivo (CTGF). Em oposição aos efeitos de TGF-'beta'1, algumas citocinas inflamatórias, incluindo interferon 'gama' (IFN'gama'), parecem inibir a transdiferenciação dos miofibroblastos. O uso da droga imunossupressora ciclosporina A (CsA) é freqüentemente associada a inúmeros efeitos colaterais, sendo o aumento gengival fibrótico o de maior interesse para a área odontológica. Linhagens celulares de fibroblastos de gengiva normal tratadas com CsA expressam níveis elevados de TGF-'gama'1 e colágeno, que são intrínsecos do fenótipo miofibroblástico. Os objetivos deste estudo foram compreender a participação de TGF-'beta'1 e IFN'gama' na transdiferenciação dos fibroblastos gengivais em miofibroblastos e entender os mecanismos de ação destes fatores neste processo. Adicionalmente, nós verificamos a presença de miofibroblastos em aumentos gengivais induzidos por CsA. Nossos resultados demonstraram, através de 4 diferentes ensaios: transcriptase reversa-reação em cadeia da polimerase (RTPCR), western blot, imunofluorescência e citometria de fluxo, que TGF-'beta'1 induz e FNg bloqueia a transdiferenciação dos fibroblastos gengivais em miofibroblastos metabolicamente ativos em uma maneira dependente da dose e tempo. Nas células onde a expressão de CTGF foi significantemente reduzida por seqüências específicas de RNA de interferência, TGF-'beta'1 não foi capaz de promover a transdiferenciação dos miofibroblastos, revelando que TGF-'beta'1 é dependente da ativação de CTGF para induzir a transdiferenciação dos fibroblastos gengivais em miofibroblastos. IFN'gama' bloqueou a transdiferenciação dos miofibroblastos por estimular a expressão de Smad 7, uma proteína que regula negativamente a atividade de TGF-'beta'1. Interessantemente, miofibroblastos não foram associados aos aumentos gengivais induzidos por CsA, como revelado pelos modelos in vivo de injeções diárias de CsA em ratos Wistar e in vitro de tratamento de fibroblastos gengivais com CsA. Embora CsA tenha induzido a expressão de TGF-'beta'1, não demonstrou nenhum efeito na modulação dos níveis de CTGF. Nossos resultados demonstram que a indução da transdiferenciação dos miofibroblastos por TGF-'beta'1 é dependente da estimulação de CTGF. Adicionalmente, este estudo sugere que IFN'gama' pode ser clinicamente efetivo no tratamento de alterações fibróticas associadas à presença de miofibroblastos, pois atenua a excessiva produção de colágeno tipo I por estas células
Abstract: Myofibroblasts are the main cellular type involved in extracellular matrix deposition in fibrotic diseases. Relatively little is known about the underlying mechanisms that regulate myofibroblast emergence (transdifferentiation), however the regulatory cytokine transforming growth factor-beta 1 (TGF-'beta'1) has been traditionally considered an inducer of the myofibroblastic phenotype via activation of connective tissue growth factor (CTGF)-dependent pathway. Some inflammatory cytokines, particularly interferon g (IFN'gama'), show opposite effects to TGF-'beta'1, inhibiting myofibroblast transdifferentiation. Cyclosporin A (CsA) is a widely used immunosuppressant drug that causes significant side effects such as gingival overgrowth. Normal gingival fibroblast cell lines treated with CsA express high levels of TGF-'beta'1 and collagen, which are intrinsic of myofibroblasts. In the present study we examined whether TGF-'beta'1 and IFN'gama' can modulate transdifferentiation of gingival fibroblasts into myofibroblasts, and the biologic mechanisms behind those factors in this process. Additionally, we have analyzed the presence of myofibroblasts in CsA-induced gingival overgrowth. Our results demonstrated throughout a modality of experiments that included reverse trancriptasepolymerase chain reaction (RT-PCR), western blot, immunofluorescence and flow citometry analysis, that TGF-'beta'1 induces and IFN'gama' blocks fibroblast-myofibroblast transdifferentiation in a dose- and time-dependent manner. In fibroblasts with CTGF levels know-down by specific small interference RNA, TGF-'beta'1 effect on transdifferentiation was significantly reduced, revealing that CTGF plays a crucial role in mediating TGF-'beta'1 myofibroblast transdifferentiation. IFN'gama' blocked myofibroblast transdifferentiation by stimulating Smad 7 levels, a protein that negatively regulates TGF-'beta'1 signaling. Interestingly, myofibroblasts were not found in CsA-induced gingival overgrowth, as revealed by the in vivo model of daily injections of CsA in Wistar rats and by the in vitro model of gingival fibroblast cultures treated with CsA. Although CsA treatment stimulated TGF-'beta'1 expression by NG fibroblasts, it lacked to induce CTGF levels. Our results demonstrate that TGF-'beta'1 induction of transdifferentiation of gingival fibroblasts to myofibroblasts occurs via a CTGF dependent pathway. Additionally, this study suggests that IFN'gama' may be clinically effective in the treatment of fibrotic diseases associate with myofibroblast
Mestrado
Patologia
Mestre em Estomatopatologia
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Mandetta, Carolina de Moraes Rego. "Avaliação in vitro de matriz colágena suína como arcabouço tridimensional para cultivo de fibroblastos gengivais." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-12072012-110036/.
Abstract:
Introdução: Fibroblastos gengivais desempenham um importante papel na regeneração de tecidos moles de proteção. Mucograft® (MCS-3D) é um substituto xenógeno novo que vem sendo utilizado na periodontia em técnicas de recobrimento radicular e aumento de tecido queratinizado. O objetivo do presente estudo foi avaliar morfológica e imunohistoquimicamente, se a MCS-3D é uma matriz tridimensional adequada, por meio de sua resposta à cultura de fibroblastos gengivais. Material e Métodos: Fibroblastos gengivais humanos (FGH) foram obtidos pela técnica do explante a partir de tecido conjuntivo gengival de três indivíduos. Os FGH foram cultivados sobre colágeno bovino bidimensional (ColB-2D), colágeno bovino tridimensional (ColB-3D) e matriz colágena suína tridimensional (MCS-3D) por até 10 dias. Em 3, 7 e 10 dias, os seguintes parâmetros foram avaliados: número, morfologia e distribuição de FGH por fluorescência direta; viabilidade celular por MTT; proliferação celular e expressão de proteínas citoesqueléticas: actina e tubulina e proteínas da matriz extracelular não colágena: fibronectina imunofluorescência indireta. Os dados quantitativos foram submetidos aos testes estatísticos de Friedman para comparação intra e intergrupos para as variáveis da análise de expressão das proteínas: fibronectina, actina e tubulina. O teste de Kruskal-Wallis foi utilizado para comparações intra e intergrupos, seguido do teste de Tukey para as variáveis das análises de viabidlidade, proliferação e número total de células. Resultados: A epifluorescência revelou que em 3 dias os FGH apresentavam-se aderidos em todos os grupos experimentais. FGH cultivados sobre ColB-2D exibiram forma menos alongada, ampla e achatada com maior quantidade de projeções e numerosas fibras de estresse em 3, 7 e 10 dias. Por outro lado, os FGH cultivados sobre ColB-3D e MCS-3D demonstraram, em todos os períodos experimentais, fenótipo fusiforme, alongado ou cilíndrico com menor quantidade de projeções do que observado no substrato 2D, com algumas das células cultivadas sobre MCS-3D demonstrando aspecto estrelado. O ensaio de MTT revelou viabilidade celular significantemente superior no ColB-2D e MCS-3D do que no ColB-3D (p<0,001), em todos os períodos experimentais. Em 3 dias, foi observado maior número de FGH cultivados sobre ColB-2D seguido por MCS-3D e ColB-3D respectivamente, havendo diferença estatística entre ColB-2D e MCS-3D (p<0,001) e entre ColB-2D e ColB-3D (p<0,001). No sétimo dia houve redução no número de células no ColB-2D e aumento na MCS-3D e ColB-3D, em que o número de células foi significantemente superior no ColB-2d com relação a MCS-3D (p=0,002). Ao final do período experimental, foi observado aumento no número de células em todos os grupos experimentais, sendo o número de células, mais uma vez, significantemente superior no ColB-2D do que na MCS-3D (p=0,002). Maior porcentagem de FGH no ciclo celular pode ser observado em ColB-2D seguido por ColB-3D e MCS-3D, respectivamente, em todos os períodos experimentais. Na MCS-3D praticamente não houve marcação por Ki-67 e o ColB-2D apresentou redução significativa de FGH ciclando entre o período de 3 e 10 dias (p=0,036). A expressão de proteínas não colágenas e citoesqueléticas foi similar em ambas as matrizes experimentais durante todo o estudo. Conclusão: Podemos concluir que a MCS-3D constitui uma matriz adequada para o cultivo de fibroblastos gengivais humanos, uma vez que, no interior da matriz, importantes propriedades foram verificadas, dentre as quais, morfologia, proliferação, e expressão de proteínas, semelhantes a uma matriz colágena tridimensional já consagrada na literatura.Introduction: Gingival fibroblasts play a central role in oral soft tissue regeneration. Mucograft® (PCM-3D) is a new xenogeneic substitute that has been used in periodontics in techniques such as root coverage and increasing keratinized tissue. The aim of this investigation was to verify if PCM-3D is a suitable three-dimensional matrix though its in vitro response to the culture of HGF. Methods: Human gingival fibroblasts (HGF) culture was established by the explant technique of gingival connective tissues of three healthy patients. HGF were seeded on bidimensional bovine collagen matrix (BCol-2D), three-dimensional bovine collagen matrix (BCol- 3D) and three-dimensional porcine collagen matrix (PCM-3D). HGF were grown for up to 10 days. At 3, 7 and 10 days the following parameters were assessed: HGF number, morphology and distribution by direct fluorescence; cell viability by MTT; cell proliferation and expression of proteins of the extracellular matrix non-collagenous fibronectina and cytoeskeletic - proteins actin and tubulin by indirect immunofluorescence. Quantitative data were submitted to Friedman and Kruskal- Wallis test, and the last one was followed by Tukeys test. Results: Epifluorescence revealed that at 3 days HGF grown on BCol-2D were broader, more flattened and had more cell protrusions and stress fibers in all experimental times. On the other hand, HGF seeded on BCol-3D and PCM-3D displayed a spindle-shaped, elongated, or cylindrical phenotype with fewer protrusions as well as less total cell spread area than on the 2D matrix, with some cells on PCM-3D showing stellate appearance. MTT assay showed cell viability higher in cultures grown on BCol-2D and PCM-3D than in BCol-3D (p<0,001). At 3days a greater number of cells in BCol-2D samples followed by PCM-3D and BCol-3D, respectively, was observed with statistical difference between BCol-2D and PCM-3D and between (p<0,001). At 7 days, there was a decrease in cell number grown on BCol-2D and an increase in BCol-3D and PCM-3D, where the number of cells were significantly higher in BCol-2D in relation to PCM-3D (p=0.002). At the end of the experimental period, there was an increase in total cell number in all of the experimental groups, and it was again significantly greater in BCol-2D than in PCM (p=0.005). Between 3 and 10 days, there was an increase in total cell number in ColB-3D (p<0.001), which showed higher counts within 10 days. Higher percentage of HGF in the cell cycle could be seen in BCol-2D followed by BCol-3D and PCM-3D, respectively, in all of the experimental groups. In PCM-3D there was almost no expression of Ki-67. BCol-2D showed a decrease in HGF cycling between 3 and 10 days (p=0,036). The expression of non-collagenous and cytoskeletal proteins were similar in both matrices during all the period of the study. Conclusion: PCM-3D is suitable for HGF culture, since, within the matrix, important properties were found, among them, morphology, proliferation andprotein expression, similar to those observed in a 3D collagen matrix well established in the literature.
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Costa, Eliane Ferraz da. "Influência de Equisetum giganteum e Punica granatum sobre os fibroblastos gengivais humanos: manutenção da viabilidade." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/25/25150/tde-16082017-174342/.
Abstract:
Estudos relataram a atividade antimicrobina de Equisetum giganteum e Punica granatum, reduzindo o risco do desenvolvimento da estomatite protética, doença relacionada principalmente à colonização das próteses pelo fungo Candida albicans. Diante disso, é necessário que essas plantas sejam bem toleradas pelos tecidos bucais e, se possível, auxiliem no processo de reparo. Objetivo: Realizar um estudo fitoquímico de substâncias potencialmente ativas de Equisetum giganteum e Punica granatum e avaliar a viabilidade de fibroblastos gengivais humanos frente a diferentes concentrações destas plantas, após diferentes períodos in vitro. Material e Métodos: Após a identificação dos compostos das plantas por HPLC-PAD, foram selecionadas as frações com melhor atividade antifúngica perante Candida albicans. Os extratos brutos e frações selecionadas de P. granatum e E. giganteum foram testados em diferentes concentrações (0.23, 0.47, 0.94, 1.88, 3.75, 7.5, 15 e 30 mg) e períodos (12, 24, 36 e 60 h) sobre fibroblastos gengivais humanos (FGH), para a avaliação de suas citotoxicidades através da análise da viabilidade dos FGH, pelo ensaio LIVE/DEAD. Por meio desse ensaio, analisamos quantitativamente a viabilidade celular através das leituras das unidades relativas de fluorescência (URF) e qualitativamente por meio da analise da morfologia e da densidade observadas pela microscopia invertida de fluorescência, após três experimentos independentes para cada período de avaliação. Os resultados quantitativos paramétricos foram representados como média e desvio padrão (SD) com análise de Variância ANOVAOne- Way, seguida da análise comparativa pelo teste de Tukey HSD. Os resultados quantitativos não paramétricos foram apresentados como mediana e quartis e foram submetidos ao teste de Kruskal-Wallis, seguido de análise comparativa pelo teste de Dunn, de acordo com as análises de normalidade (Teste de Kolmogorov-Smirnov). Valores de p< 0.05 foram indicativos de significância estatística. Conclusões: Foram identificados compostos fenólicos, como flavonoides e taninos em E.giganteum e P. granantum, respectivamente. Identificamos uma citotoxicidade concentração-dependente para as duas plantas testadas, independente do tempo. Sob as menores concentrações das plantas a viabilidade e a morfologia dos FGH foram preservadas. Dentro deste contexto, acreditamos na possibilidade de se utilizar essas plantas como terapia alternativa em diversas áreas de saúde, porém em concentrações biocompatíveis.Studies have reported the antimicrobial activity of Equisetum giganteum and Punica granatum, reducing the risk of development of denture stomatitis, a disease mainly related to the colonization of prostheses by the fungus Candida albicans. In view of this, it is necessary that these plants be well tolerated by the oral tissues and, if possible, assist in the repair process. Objective: To carry out a phytochemical study of potentially active substances of E. giganteum and P. granatum and to evaluate the viability of human gingival fibroblasts against different concentrations of these two plants, after different periods in vitro. Material and Methods: After the identification of the plant compounds by HPLC-PAD, the fractions with the best antifungal activity against Candida albicans were selected. The crude extracts and selected fractions of P. granatum and E. giganteum were tested at different concentrations (0.23, 0.47, 0.94, 1.88, 3.75, 7.5, 15 and 30 mg) and periods (12, 24, 36 and 60 h) against human gingival fibroblasts (FGH), for the evaluation of their possible cytotoxicity through the analysis of the viability of FGH by the LIVE/DEAD assay. By means of this assay we quantitatively analyzed the cell viability through the readings of the relative units of fluorescence (URF) and qualitatively by analysis of the cellular morphology and density using inverted fluorescence microscopy, after three independent experiments. The parametric quantitative results were represented as mean and standard deviation (SD) with ANOVA-One-Way Variance analysis, followed by comparative analysis by the Tukey HSD test. The non-parametric quantitative results were presented as medians and quartiles and were submitted to the Kruskal-Wallis test, followed by comparative analysis by the Dunn test, according to normality tests (Kolmogorov-Smirnov test). Values of p <0.05 were indicative of statistical significance. Conclusions: Phenolic compounds, such as flavonoids and tannins, were identified in E. giganteum and P. granantum, respectively. We identified a concentration-dependent cytotoxicity for the two plants tested, regardless of the time. Under the lowest concentrations of plants the viability and morphology of FGH were preserved when compared to the control. Within this context, we believe in the possibility to use these plants as alternative therapy in several health areas, but in biocompatible concentrations.
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Maia, Luciana Prado. "Avaliação in vitro de matriz dérmica acelular como arcabouço tridimensional para cultivo de fibroblastos gengivais." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-27082010-160945/.
Abstract:
Fibroblastos gengivais desempenham um importante papel na regeneração de tecidos moles de proteção do periodonto. Alloderm® (MDA) é um substituto alógeno muito utilizado e estudado em periodontia. O objetivo do presente estudo foi avaliar, in vitro, se a MDA é uma matriz tridimensional adequada, através de sua resposta à cultura de fibroblastos gengivais e células neoplásicas; e, ainda, se os subprodutos da MDA influenciam o comportamento celular. Material e Métodos: Fibroblastos gengivais de cão (FGC) e fibroblastos gengivais humanos (FGH) foram obtidos pela técnica do explante a partir de tecido conjuntivo gengival de, respectivamente, três indivíduos e três cães saudáveis. As células FGC, FGH e B16F10 de melanoma murino foram cultivadas sobre a MDA por até 14 dias. Os seguintes parâmetros foram avaliados: presença, morfologia e distribuição de FGC, FGH e B16F10 por fluorescência direta; viabilidade de FGC e FGH por MTT; e o efeito do meio de cultura condicionado (MC) em MDA por 24 h na viabilidade celular de FGC por MTT. Os dados quantitativos foram submetidos aos testes estatísticos Mann-Whitney e Kruskal-Wallis, seguido pelo método de Dunn para comparações múltiplas (nível de significância: 5%). Resultados: A epifluorescência revelou que, em 12 h, FGH e FGC estavam aderidos à superfície da MDA em baixa densidade celular, exibindo morfologia poligonal com núcleos esféricos, enquanto que, aos 7 e 14 dias, essas células apresentavam com formato alongado, núcleos ovalados e citoesqueleto de actina com fibras de estresse. Aos 7 e 14 dias, FGC apresentavam-se distribuídos de forma desigual sobre a MDA, formando uma camada celular descontínua, sem aumento no número de células entre os períodos; FGH formaram uma monocamada celular na superfície da MDA, estando presentes em maior número após 14 dias de cultivo (p<0,05); e B16F10 exibiram um aumento no número de células de 12 h para 7 dias (p<0,05), apresentando-se dispostas em aglomerados celulares, principalmente na superfície da MDA, com a formação de camada contínua aos 14 dias. Notou-se maior número de células nas amostras cultivadas com B16F10, seguido por FGH e FGC aos 7 dias (p<0,05). Aos 14 dias, FGH e B16F10 estavam presentes em maior número, com diferença estatística significante em relação aos FGC (p<0,05). Foi observada maior porcentagem de células na superfície (p<0,05) do que no interior da MDA e essa proporção manteve-se estável durante os períodos avaliados para todos os tipos celulares. O ensaio de MTT indicou maior viabilidade celular nas amostras cultivadas com FGH comparado a FGC (p=0,024), aos 7 e 14 dias. Notou-se um decréscimo na viabilidade celular em culturas cultivadas em MC, com diferença estatística entre os grupos em 48 e 72 horas (p<0,05). Conclusão: Podemos concluir que fibroblastos gengivais e mesmo células altamente proliferativas, como B16F10, povoam apenas superficialmente a MDA e que FGC são afetados negativamente pelos subprodutos da MDA, reduzindo sua viabilidade.Gingival fibroblasts play a central role in oral soft tissue regeneration. Alloderm® (Alloderm® - ADM) is the most used and studied allogeneic substitute. The aim of this investigation was to verify if ADM is a suitable threedimensional matrix, through its in vitro response to gingival fibroblasts and cancerous cells lineage and, also, if ADM end products affect cellular behavior. Methods: Canine gingival fibroblasts (CGF) and human gingival fibroblasts (HGF) cultures were established by the explant technique of gingival connective tissues of three dogs and three healthy patients, respectively. CGF, HGF and B16F10 cells of murine melanoma were seeded on ADM and grown for up to 14 days. The following parameters were assessed: presence, morphology and distribution of CGF, HGF e B16F10 by direct fluorescence; CGF and HGF viability by MTT; and the effect of culture medium conditioned (CM) in the MDA for 24 h on CGF viability by MTT. Quantitative data were submitted to Mann-Whitney and Kruskal-Wallis tests, followed by Dunn\'s method. Results: Epifluorescence revealed that CGF and HGF were adherent and exhibited a polygonal morphology at 12 h while at 7 and 14 days they were spread, exhibiting an elongated shape and the actin cytoskeleton assembled into stress fibers. CGF were unevenly distributed on ADM surface, showing no increase in cell number over the experimental periods; HGF formed a monolayer on the ADM surface, in a higher number at 14 days (p<0,05); B16F10 exhibited na increase in cell number in 7 days (p<0,05), and were mainly arranged in cell aggregates on the ADM, forming a continuous layer at 14 days. A higher percentage of cells on the ADM surface (p <0.05) compared to inside the matrix was observed for all cell types in all periods. MTT values indicated higher cell viability in samples cultured with HGF compared to CGF (p=0.024). A significantly lower cell viability for CGF grown in CM compared to cells grown in non conditioned medium at 48 and 72 h (p <0.05) was noticed. Conclusion: Gingival fibroblasts and even highly proliferative cells as B16F10 can be only superficially located on ADM and CGF are negatively affected by ADM end products, reducing its viability.
33
Pfeiffer, Friederike [Verfasser]. "Untersuchung des Adhäsionsverhaltens von Gingiva-Fibroblasten auf mikrostrukturierten Titanoberflächen / vorgelegt von Friederike Pfeiffer." Tübingen, Engelfriedshalde 85 : F.Pfeiffer, 2004. http://d-nb.info/971387761/34.
34
Palm, Eleonor. "Inflammatory responses of gingival fibroblasts in the interaction with the periodontal pathogen Porphyromonas gingivalis." Doctoral thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-38660.
35
Harris, Jesse. "Comparison of STERIPLEX™ HC and Sodium Hypochlorite Cytotoxicity on Primary Human Gingival Fibroblasts." VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/2662.
Abstract:
This study examined the cytotoxic effects of STERIPLEX™ HC (sBioMed, Orem, UT) and sodium hypochlorite (NaOCl) on human fibroblast cells in vitro. Fibroblasts exposed to various concentrations of NaOCl or STERIPLEX™ HC were visualized via light microscopy. Dilutions of either NaOCl or STERIPLEX™ HC that did not appear to disrupt the integrity of the cells were recorded for further analysis. Cells were then cultured and grown to confluence in five separate plates. A void was created down the middle of each plate. If the cells were viable, cellular confluence was seen. If nonviable, confluence of the cells did not occur. Both disinfectants showed absolute kill at all concentrations above 1%. The cells treated with 0.1% NaOCl were found to be nonviable. However, at 0.1% STERIPLEX™ HC, the cells were viable and able to replicate, filling the void and returning to confluence.
36
Barona, Intriago Maria Fernanda. "Regulation of gingival fibroblast gene expression by leukocyte and platelet rich fibrin." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62647.
Abstract:
Objectives: Leukocyte and Platelet-Rich Fibrin (L-PRF), a new clinically used blood product, is rich in leukocytes and platelets embedded in a high-density fibrin network. L-PRF has shown some clinical promise to promote gingival wound healing. In gingival surgeries where L-PRF is being used, it will be in direct contact with human gingival fibroblasts (HGFs) of the surgical flap. However, little is known about the combined effects of the growth factors and cells present in L-PRF on HGFs. We hypothesized that L-PRF regulates expression of key wound healing genes in HGFs. Thus, our aim was to investigate the effects of soluble biological factors released from L-PRF on HGF gene expression.
Methods: L-PRF membranes were produced from blood samples of six volunteer donors using standard clinical protocol. The membranes were placed into upper compartments of transwell plate inserts (0.4 µm pore size) with confluent layers of HGFs at the bottom of the wells. Thus, only soluble mediators were able to influence HGF gene expression. HGFs with the L-PRF membranes in the wells were incubated for 48h followed by isolation of total RNA for real-time quantitative PCR of wound healing related genes. The levels of selected proteins produced by the cells were analyzed using Western blotting.
Results: Among the 86 genes studied, the expression of 35 genes (41%) was significantly regulated by the L-PRF membranes in HGFs. Replicate membranes from the same donor or between different donors produced similar responses, with membranes from different individuals varying somewhat in magnitude of the response. Among the most regulated genes, matrix metalloproteinase-1 (MMP-1) and MMP-3 showed several-fold increase by L-PRF-treatment, both at the mRNA and protein level. Angiogenesis related genes, VEGF-α and FGF-2 showed also significant up-regulation in HGFs by the L-PRF-treatment.
Conclusions: The results demonstrate that the L-PRF membranes have a strong and a specific regulatory effect on HGFs that may modulate several aspects of wound healing.Dentistry, Faculty of
Graduate
37
Kunze, Melanie. "Effizienter Gentransfer in humane parodontale Ligamentzellen und humane gingivale Fibroblasten mittels Adeno assoziierter Virus Vektoren." Köln Deutsche Zentralbibliothek für Medizin, 2010. http://d-nb.info/1000932621/34.
38
Kelsey, William Patrick V. "Proteomic Analysis of the Nuclear Membranes of Human Periodontal Ligament Fibroblast and Gingival Fibroblast Cell Types: A Comparison Study." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1243618431.
39
Hanino, Mohammad. "Mechanism of ciclosporin-induced gingival hyperplasia : an in vitro study of the effect of ciclosporin on human gingival fibroblasts and oral keratinocytes." Thesis, Queen Mary, University of London, 2011. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1297.
Abstract:
Objectives: Ciclosporin (CsA) is a potent and effective immuno-suppressive drug, but its use is associated with the development of gingival hyperplasia in up to 70% of patients. The mechanism underlying this side effect is unknown and the aim of this in vitro study was to investigate the direct effect of CsA on oral epithelium and connective tissue and to explore whether oral bacterial products modified the response. Methods: 3 human oral and gingival keratinocyte cell lines (OKF6, TR146 and FIBS) and a human gingival fibroblast cell line (HGF) along with reconstructed human gingival and oral epithelial models (RHGE and RHOE respectively, SkinEthic) were used in the study. Cells/tissues were pre-stimulated for 24h with bacterial supernatants (P. gingivalis (Pg), A. actinomycetemcomitans (Aa), F. nucleatum (Fn), and P. intermedia (Pi) at 1/100 or 1/10 respectively and then exposed to a combination of CsA (2000 or 250 ng/ml) and bacterial supernatant for further 24h or 48h. Cell viability, cytokine/ chemokine (IL-1α; IL-6; IL-8) release; cell cycle and proliferation markers (DNA synthesis, cyclin B1, cyclin D1, CCNB1, and CCND1) and apoptosis (Bax, Bcl-2 protein and gene, and Fas-L gene) were assessed using MTT assay, ELISA, FACs analysis, quantitative PCR. Cell cycle analysis, DNA synthesis and expression of cyclins D1 and B1 in cell lines were evaluated simultaneously. Results: CCNB1 and CCND1 were significantly up-regulated in all RHGE cultures exposed to any of the combinations applied compared to controls (CCNB1: CsA+A.a; 1.543± 0.039; CsA; 0.703± 0.032; A.a; 0.669± 0.002; untreated control; 1± 0.047)(CCND1; CsA+A.a; 3.41± 22 versus CsA; 1.046± 0.079; A.a; 2.127± 0.22; and untreated 3 control; 1± 0.106). FACS analysis of monolayer cultures showed an apparent increase in proliferation rate, illustrated by DNA synthesis, of FIBS, OKF6, and HGF consistently with the combination (P.i plus 2000ng/ml CsA) compared to controls. CsA at 2000ng/ml caused a significant increase in cytokine release from both keratinocyte and fibroblast cell lines. Furthermore, bacterial products in combination with CsA had a synergistic effect on IL-6 and IL-8 release from HGF but had a little effect on cytokine release from keratinocytes. Conversely, the combined treatments significantly decreased IL-8 in RHOE. Conclusion: The results suggest that a clinically relevant dose of CsA (2000ng/ml) along with bacterial products stimulate the proliferation of gingival keratinocyte and HGF by activating the cell cycle and DNA replication. Thus, the presence of specific periodontal microorganisms may be important in the development of the condition
40
Alanazi, Humidah. "Effect of cigarette smoke on "Candida albicans" growth and its interaction with human gingival fibroblasts." Master's thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26367.
Abstract:
L’objectif principal de cette étude est d’étudier l’effet de la fumée de cigarette (FC) sur C. albicans et sur son interaction avec les fibroblastes gingivaux humains. Nous avons démontré que Candida se multiplie présence FC, en adoptant la forme hyphe. La FC rende C. albicans sensible au H2O2, mais résistant aux la chaleur et au NaCl. La FC augmente la production de chitine par C. albicans. Préincubé avec le FC, C. albicans adhère beaucoup plus aux cellules gingivales en monocouche, prolifère plus et adopte la forme hyphe plus facilement. Les fibroblastes quant à eux ils montrent une réduction de leur croissance, mais produisent plus de l’IL-1b. En conclusion: le CFC module d’un côté C. albicans et de l’autre côté les cel-lules de l’hôte. Ceci suggère un négatif important de la fumée de cigarette favorisant les candidoses orales chez les fumeurs.The main objective of this study was to investigate the effect of cigarette smoke (CS) on C. albicans and its interaction with human gingival fibroblasts. We have shown that Candida multiplies presence CS, by adopting the hyphae form. CS makes C. albicans sensitive H2O2, but resistant to heat and NaCl. CS in-creases chitin production by C. albicans. Preincubated with CS, C. albicans adheres more to gingival cells in monolayer, proliferating more and adopts the hyphae form more easily. Fibroblasts show a reduction of their growth, but produce more IL-1b. CSC modulates C. albicans and on the other side also CS modulates the host cells. This suggests a significant negative cigarette smoke promotes oral candidiasis in smokers.
41
Ramírez, Rámiz Albert. "Estudio celular y molecular en cultivos de fibroblastos tratados con fármacos inductores de agrandamiento gingival." Doctoral thesis, Universitat de Barcelona, 2007. http://hdl.handle.net/10803/689.
Abstract:
El Agrandamiento gingival farmacológico -AGIF- es un dismorfismo gingival provocado por la inducción de fármacos antiepilépticos -Fenitoína-, antagonistas del calcio -Nifedipina- e inmunosupresores -Ciclosporina-. En los primeros casos de AGIF, se creía que la alteración histopatológica residía en un aumento de la población celular de los fibroblastos -Hipótesis nula-. Este estudio quiere demostrar que esta alteración se produce en la matriz extracelular -fibras colágenas y glicosaminoglicanos de la sustancia fundamental:
Los fármacos inductores del Agrandamiento gingival no provocan una hiperplasia gingival secundaria a la proliferación celular de fibroblastos -Hipótesis alternativa-.
Para ello se proponen unos objetivos:
1-Determinar si cultivos de fibroblastos de encía humana son sensibles a la acción de fármacos inductores de Agrandamiento gingival.
2-Observar el efecto de estos fármacos en cultivos primarios de fibroblastos procedentes de las muestras examinadas -estudio de la viabilidad celular-.
3-Cuantificar si hay efecto farmacológico en la transcripción génica del Factor de Crecimiento Transformador ß (TGFß), del colágeno y de la colagenasa.
4-Observar si hay efecto farmacológico en la síntesis del colágeno y del TGFß.
Se tomaron muestras gingivales de pacientes, una de ellas sin relación con la administración de fármacos inductores conocidos de Agrandamiento gingival. Otra muestra se tomó de un paciente transplantado renal sometido desde hacía 2 años a ciclosporina -125 mg/12h-. Se observaronn las muestras a microscopía óptica para ver las características histopatológicas y una porción se fragmentó en explantes y se cultivó en medio esencial para conseguir un número determinado de fibroblastos. Se aplicaron los tres fármacos inductores a unas dosis preestablecidas -según estudios in Vitro consultados- y se observó la Viabilidad de los fibroblastos para el análisis de la proliferación celular. En una segunda fase, se estudió la expresión del mRNA de tres proteínas implicadas en la patogenia del AGIF -colágeno, colagenasa y TGFβ-. Finalmente se observó la traducción proteica del colágeno y del TGFβ.
Los resultados permitieron comprobar que no se produjeron cambios en la proliferación fibroblástica para la inducción con nifedipina y ciclosporina. Fenitoína produjo una reducción de esta proliferación como si hubiera un efecto tóxico del fármaco. En la transcripción génica de las proteínas consideradas se observaron aumentos para los tres fármacos inductores. En la expresión proteica del colágeno tampoco se apreciaron cambios.
En base a las muestras analizadas se puede considerar que hay una afectación en la matriz extracelular por la inducción farmacológica al comprobar aumentos significativos en mRNA de colágeno, TGFβ y colagenasa. Aunque hay factores que pueden actuar en la post-transcripción que alteren la expresión de estas proteínas y la actividad de la colagenasa.
Las conclusiones del estudio han sido:
1. Los fibroblastos gingivales de cultivos primarios son sensibles a fármacos inductores de Agrandamiento gingival -fenitoína, nifedipina y ciclosporina-.
2. Los fármacos inductores de Agrandamiento gingival no provocan hiperplasia gingival secundaria a la proliferación celular de fibroblastos.
3. Los fármacos inductores provocan un incremento significativo de la transcripción del TGFß, colágeno y colagenasa.
4. Los fármacos inductores no producen un incremento de la traducción del colágeno, con repercusión en la matriz extracelular.
42
Yang, Po Fong. "Filamentous actin disruption and diminished inositol phosphate response in gingival fibroblasts caused by Treponema denticola." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0007/MQ40757.pdf.
43
Chaussain-Miller, Catherine. "Rôle du fibroblaste dermique et gingival dans la cicatrisation : modulation de l'organisation matricielle in vitro." Paris 5, 2001. http://www.theses.fr/2001PA05M052.
Abstract:
La peau et la gencive, de par leur localisation, sont exposés à de multiples agressions. La cicatrisation gingivale est plus efficace que la cicatrisation cutanée et se traduit en particulier par une régénération tissulaire quasi ad integrum. Nous avons tenté de mieux comprendre certains mécanismes intervenant dans la cicatrisation du derme et du tissu conjonctif gingival en cultivant des fibroblastes issus de peaux et de gencives humaines en lattis de collagène. . .
44
Fournier, Benjamin. "Thérapie cellulaire de l'anévrisme aortique par le fibroblaste gingival : études ex vivo et in vivo." Paris 5, 2009. http://www.theses.fr/2009PA05T019.
Abstract:
L'anévrisme aortique abdominal s'accompagne d'une dégradation du réseau élastique et d'une augmentation des métalloprotéases. Nous avons essayé de transposer les qualités de réparations du fibroblaste gingival sur ces artères dans des modèles ex-vivo et in vivo. Un modèle de culture d'artère de lapin en gel de collagène est évalué puis utilisé en coculture avec des fibroblastes gingivaux pour évaluer l'effet de ces fibroblastes sur le remodelage artériel. Les fibroblastes gingivaux sont également cultivés avec des artères issues d'anévrismes aortiques humains. Enfin notre hypothèse est testée sur un modèle d'anévrisme chez le lapin où les cellules sont transférées par voie endoluminale. En coculture, les fibroblastes gingivaux inhibent la MMP-9 par une augmentation de son inhibiteur le TIMP-1. La même inhibition est présente dans des cocultures avec des artères anévrismales humaines. La MMP-7 est également inhibée par augmentation du TIMP-1 mais aussi au niveau transcriptionnel par une augmentation du TGF-pl. Ces cocultures permettent la préservation du réseau élastique artériel. Le transfert des fibroblastes dans des anévrismes créés chez le lapin entraîne la diminution de leurs diamètres et de la MMP-9. Ces résultats obtenus sur des modèles ex vivo et in vivo montrent la capacité des fibroblastes gingivaux à préserver le réseau élastique et à moduler l'activité de protéases impliquées dans la pathologie. La transplantation de fibroblastes gingivaux semble être une approche intéressante dans le traitement des anévrismes aortiques. Néanmoins des expériences complémentaires sont nécessaires pour confirmer nos résultats et comprendre comment le fibroblaste gingival influe positivement le remodelageAortic abdominal aneurysm is accompanied by a degradation of the elastic network and an increase of the metalloproteinases. We tried to transpose repair qualities of the gingival fibroblast on these arteries in ex-vivo and in vivo models. A culture model of rabbit artery in collagen gel is evaluated then used in coculture with gingival fibroblasts to evaluate the effect of these fibroblasts on arterial remodeling. The gingival fibroblasts are also cultivated with human aneurismal aortas. Finally our hypothesis is tested on an in vivo model of rabbit aneurism where the cells are transplanted into the lumen. In coculture, the gingival fibroblasts inhibit MMP-9 by an increase of its inhibitor: TIMP-1. Same inhibition is present in cocultures with human aneurismal aortas. The MMP-7 is also inhibited by increase in the TIMP-1 but also at a transcriptional level by an increase of TGF-pl. These cocultures allow the preservation of the arterial elastic network. The transfer of the fibroblasts in aneurisms created in rabbit reduced their diameters and the MMP-9. These results obtained on ex vivo and in vivo models show the capacity of the gingival fibroblasts to preserve the elastic network and to modulate the activity of proteases implied in pathology. The transplantation of gingival fibroblasts seems to be an interesting approach in the treatment of aortic aneurisms. Nevertheless complementary experiments are necessary to confirm our results and to understand how the gingival fibroblast influences remodeling
45
Alamri, Abdullah, and Abdullah Alamri. "Exposure to cigarette smoke condensate and its impact on human gingival fibroblast: mechanisms of molecular pathways involved in survival/apoptosis." Master's thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26042.
Abstract:
Notre objectif est d'étudier les effets de la fumée de cigarette (FC) sur les fibroblastes gingivaux humains. Nos travaux démontrent que FC réduit la viabilité cellulaire cellules vivantes par le biais de la voie apoptotique/nécrotique. Une analyse génomique a montré une surexpression significative de plusieurs gènes dont les gènes de Bax, récepteurs du TNF et caspases; mais une régulation des gènes Lymphotoxin alpha, BCLA1 et BIRC3. Une analyse protéique montant une augmentation des protéines Bax et p53 et de la caspase -3 confirmant l’effet nocif du FC biais un mécanisme apoptotique/nécrotique. Une exposition répétée au FC pendant de courtes périodes, favorise la prolifération cellulaire en augmentant l'activité de la télomérase. En conclusion, ces résultats démontrent qu’à hautes doses la FC est toxique mais à faibles doses la FC provoque une surcroissance cellulaire qui pourrait contribuer au développement des maladies parodontales et des caries.Notre objectif est d'étudier les effets de la fumée de cigarette (FC) sur les fibroblastes gingivaux humains. Nos travaux démontrent que FC réduit la viabilité cellulaire cellules vivantes par le biais de la voie apoptotique/nécrotique. Une analyse génomique a montré une surexpression significative de plusieurs gènes dont les gènes de Bax, récepteurs du TNF et caspases; mais une régulation des gènes Lymphotoxin alpha, BCLA1 et BIRC3. Une analyse protéique montant une augmentation des protéines Bax et p53 et de la caspase -3 confirmant l’effet nocif du FC biais un mécanisme apoptotique/nécrotique. Une exposition répétée au FC pendant de courtes périodes, favorise la prolifération cellulaire en augmentant l'activité de la télomérase. En conclusion, ces résultats démontrent qu’à hautes doses la FC est toxique mais à faibles doses la FC provoque une surcroissance cellulaire qui pourrait contribuer au développement des maladies parodontales et des caries.
46
HOSHINO, TAKESHI, TARO HAYAKAWA, KYOKO YAMASHITA, KOJI NISHIO, and HANG LI. "CELL CYCLE-DEPENDENT LOCALIZATION OF TISSUE INHIBITOR OF METALLOPROTEINASES-1 IMMUNOREACTIVITY IN CULTURED HUMAN GINGIVAL FIBROBLASTS." Nagoya University School of Medicine, 1995. http://hdl.handle.net/2237/16089.
47
Battikhi, Tulin. "Treponema Denticola outer membrane extract enhances the phagocytosis of collagen coated-beads by human gingival fibroblasts." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0026/MQ40753.pdf.
48
Mustafa, Manal. "Studies on uptake and effect of triclosan on production of inflammatory mediators in human gingival fibroblasts /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-714-2.
49
Kunze, Melanie [Verfasser]. "Effizienter Gentransfer in humane parodontale Ligamentzellen und humane gingivale Fibroblasten mittels Adeno assoziierter Virus Vektoren / Melanie Kunze." Köln : Deutsche Zentralbibliothek für Medizin, 2010. http://d-nb.info/1000932621/34.
50
Miranda, i. Rius Jaume. "Estudi de prevalença del dismorfisme gingival, engrandiment gingival en pacients tractats: amb bloquejadors dels canals del calci." Doctoral thesis, Universitat de Barcelona, 1997. http://hdl.handle.net/10803/1186.
Abstract:
Conceptualment el dismorfisme defineix una alteració morfològica, congènita o adquirida, d'una part o de la totalitat d'un aparell o d'un òrgan. Així l'engrandiment gingival és un dismorfisme per excés d'aquest teixit que s'ha relacionat amb diferents factors: inflamatoris, farmacològics, neoplàsics i d'altres.Clàssicament els fàrmacs que s'havien relacionat amb el sobrecreixement gingival eren els antiepilèptics, fonamentalment la fenitoïna. A partir de la dècada dels vuitanta aquesta alteració morfològica també s'ha relacionat amb l'administració d'altres fàrmacs com la ciclosporina A i els bloquejadors dels canals del calci: dihidropiridines, verapamil i diltiazem.
L'engrandiment gingival induït per fàrmacs presenta unes característiques clíniques i anàtomo-patològiques similars, tot i tractar-se de fàrmacs amb una acció terapèutica molt diferent. Això ha fet pensar a molts autors de l'existència d'alguna característica comuna entre ells que expliqués la patogènia del sobrecreixement gingival. En la literatura existeixen nombroses hipòtesis que pretenen explicar aquest dismorfisme, y sembla ser que el mecanisme patogènic seria la combinació de diferents factors: placa bacteriana, presència de fibroblastes gingivals predeterminats genèticament "responder" i l'acció del propi fàrmac. Una característica farmacodinà.mica comuna dels fàrmacs reconeguts com inductors de l'engrandiment gingival és la d'alterar el flux de calci transmembrana, que alhora modificaria el metabolisme dels fibroblastes del teixit connectiu gingival, produint-se un increment dels components de la matriu extracel.lular: fibres de col.làgena i/o substància fonamental amorfa. És per això que el concepte que millor definiria aquesta alteració des d'una vessant clínica seria el d'engrandiment o sobrecreixement gingival, encara que el terme hiperplasia gingival hagi estat utilitzat en la majoria de publicacions científiques.
El sobrecreixement gingival que habitualment s'inicia a la regió de la papil.la interdental, pot afavorir l'aparició de símptomes i signes clínics que inclouen: dolor, sagnat i friabilitat dels teixits, moviments anormals de les dents, alteracions estètiques, fonètiques i de l'oclusió, a més a més d'afavorir l'aparició de càries i d'altres disfuncions periodontals. Constatàrem que la majoria d'individus que presentaven engrandiment gingival aquest havia passat desapercebut tant pel pacient com pel clínic, encara que els afectats per un sobrecreixement més sever si que mañifestaren alguns d'aquests símptomes. L'engrandiment gingival induït per fàrmacs, i en el nostre cas pels calcioantagonistes, s'ha demostrat que pot ser reduït o previngut amb un bon control de la placa bacteriana dirigit a eliminar la inflamació gingival; i en els casos més severs la cirurgia periodontal resectiva és el mètode utilitzat per l'eliminació de l'excés de teixit.
La majoria de publicacions referents a l'engrandiment gingival induït per calciantagonistes, són series de casos amb un nombre reduït de pacients que els realment necessaris en funció de la prevalença estimada per aquest dismorfisme; tampoc s'hi reflecteixen valors que indiquin el risc atribuïble al consum d'aquests fàrmacs, ni comparacions respecte a una població control.
Desenvolupàrem un estudi clínic transversal en l'àmbit de l'atenció primària, CAP Rambla de Terrassa (Barcelona), on s'examinaren els pacients que de forma consecutiva eren visitats en les consultes de medicina general i cardiologia, per conèixer la prevalença, severitat i risc d'engrandiment gingival en la població tractada amb calciantagonistes, i que es comparà amb una població que no rebia cap dels fàrmacs reconeguts com inductors. En l'estudi s'inclogueren 265 pacients: 72 tractats amb dihidropiridines fonamentalment la nifedipina, 32 amb diltiazem, 14 amb verapamil i els 147 subjectes restants foren els controls de la mostra. Es valorà el grau de sobrecreixement gingival en un sentit àpico-coronari (vertical) amb l'índex d'Angelopoulos i Goaz modificat per Miller i Damm -GO-, i en un sentit àntero-posterior (horitzontal/nòdul-papil.lar) amb l'índex de Seymour modificat per Miranda i Brunet MB-. Es considerà que existia engrandiment gingival quan el valor d'algun dels dos índexs era >0 en qualsevol localització. També s'enregistraren: l'índex gingival, l'índex de placa i la profunditat de sondatge, així com les variables sòcio-demogràfiques i qualitatives de tots els individus. Estadísticament s'establiren les diferents comparacions de les variables analitzades amb la prova de Chi Quadrat i quan fou necessari s'utilitzà el Test exacte de Fischer. Es realitzà l'anàlisi bivariant dels factors que poguessin exercir alguna influència sobre els índexs de sobrecreixement gingival. S'evaluà mitjançant un anàlisi mu1tivariant de regressió logística l'Odds Ratio o risc atribuïble al calciantagonista ajustat per les variables de confusió. I finalment es determinà el test de concordança Kappa dels dos índexs utilitzats en la valoració de l'engrandiment gingival (GO i MB).
La prevalença d'engrandiment gingival en la població control fou del 4.1% per l'índex GO i del 7.5% pel d'MB. La prevalença d'engrandiment pel grup casos -bloquejadors dels canals del calci- dels diferents subgrups farmacològics fou: per les dihidropiridines 33.3% (GO) i 51.4% (ME); pel diltiazem 31.3% (GO) i 50% (MB); i pel verapamil 21.4% (GO) - 35.7% (MB); siguent les localitzacions anteriors ínfero-vestibular i súpero-vestibular les més freqüents per ambdós registres. Degut a que l'inici del sobrecreixement habitualment es localitza a nivell de la papil.la interdental, i degut a que l'índex d'MB enregistra l'engrandiment en aquesta regió, això ens explicaria aquesta diferència de freqüències.
En el subgrup de pacients tractats amb nifedipina (n=65) es relacionà la dosi acumulada d'aquest fàrmac amb el grau de sobrecreixement, i encara que a dosis acumulades altes existia una major prevalença d'engrandiment per ambdós índexs, aquest dismorfisme no el podem considerar dosi-depenent. L'edat i el sexe no semblen ser factors determinants en el desenvolupament d'aquest dismorfisme; tampoc per les altres variables qualitatives registrades. Pel que fa a la salut gingival els valors elevats de l'índex gingival (inflamació severa) condicionaren de forma significativa la presència d'engrandiment gingival. Respecte a l'acúmul de placa bacteriana, ambdós grups -casos i controls- presentaren uns índexs similars.
Ja que l'engrandiment induït per fàrmacs és per tant un engrandiment combinat, explicable per la pròpia acció del fàrmac i a la inflamació gingival sobreafegida, és important determinar en quina proporció aquest engrandiment és provocat per un factor o un altre. D'aquesta manera en el grup tractat amb dihidropiridines, quan no es considera la dosi, el risc atribuible al fàrmac seria de 10.6-14.4 (GO-MB) i l'atribuïble a l'alteració de la salut gingival seria de 9.6 per ambdós índexs. En el subgrup tractat amb nifedipina a dosi acumulada alta, el risc atribuïble a aquest fàrmac fou superior, entre 17.4-23.6 (GO-MB) i el relacionat amb la salut gingival es mantingué .invariable per ambdós índexs (9.4). Respecte als altres calciantagonistes, diltiazem i verapamil, el risc fou més superior per l'alteració de la salut gingival que no pas per la presència del fàrmac inductor; aquest fet podria ser degut segurament a la reduïda grandària de la mostra d'aquests dos grups. Es varen detectar diferències de freqüencia d'engrandiment per ambdós índexs; l'índex MB detectava un sobrecreixement en les fases incipients de l'engrandiment que no diagnosticava l'índex GO, encara que després de determinar el grau de concordança o Kappa constàrem que ambdós índexs eren fiables.
L'engrandiment gingival és un dismorfisme habitualment poc conegut pels clínics responsables d'aquests pacients; és per això important difondre valors de prevalença i risc de sobrecreixement gingival pel bon control de la salut periodontal en la població tractada amb bloquejadors dels canals del calci. En un futur caldrà analitzar mostres més àmplies de pacients tractats amb el diltiazem i sobretot amb el verapamil, per així poder determinar les freqüències de sobrecreixement gingival i valorar altres aspectes estudiats amb la nifedipina. Finalment és de suposar que el major consum de dihidropiridines diferents de la nifedipina, permetrà determinar si aquests també són fàrmacs inductors, tal com ja alguns autors estableixen en estudis experimentals i en casos clínics aïllats, i alhora establir en cada cas la seva prevalença.
Gingival enlargement is an abnormal growth of the gingival tissue. It is mainly associated with dental-plaque-related inflammation, some diseases and drug therapy. It has been reported with the use of phenytoin, cyclosporin, and calcium channel antagonist, including dihydropyridinederivatives, veraparnil and diltiazem. This research was designed to study the prevalence of gingival enlargement in general population and in patients taking calcium antagonists. Subjects (n=265) were selected using predefined inclusion-exclusion criteria. The non-treated-control group included 147 patients attended in a general practice center (none had been treated with calcium antagonists). The treated-group included 118 patients taking calcium antagonists (at inclusion time and at least during the last six months). It included 72 patients taking dihydropyridines (65 receiving nifedipine), 32 treated with diltiazem, and 14 with verapamil. Medical history and a complete oral and odontological exploration were done in all patients. It included plaque index, gingival index, probing depth and measures of gingival enlargement in the vertical (GO) and the horizontal (MB) directions. The prevalence of gingival enlargement in the non-treated-control group was 4.1-7.5% when measured by GO and MB, respectively. The patients receiving dihydropyridines had an enlargement prevalence of 33.3-51.4%. Those under diltiazem were 31.3-50% and in the case of verapamil 21.4-35.7%. The most frequents locations for both GO/MB indexes were the anterior lower and the upper-buccal jaw. In the case of nifedipine, the enlargement showed a dose/response relationship when total drog exposition was considered. High rates of gingival index (severe inflammation) produced significant differences in relation to gingival enlargement (p<0.01). No differences were observed in the plaque index. Age and gender did not seem to be determinant factors in the development of overgrowth, the same results were obtained for all other qualitative variables registered. Multivariate statistical analysis showed that the attributable risk (Odds Ratio, OR) of gingival overgrowth related to dihydropyridine treatment was 10.6-14.4 (OR for GO/MB), and the risk related to gingival inflammation was 9.6 for both GO/MB.