Relevant bibliographies by topics / Fibroblastes gingivaux

Academic literature on the topic 'Fibroblastes gingivaux'

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Published: 7 July 2024
Last updated: 7 July 2024

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Journal articles on the topic "Fibroblastes gingivaux":

1

Lauritano, Dorina, Alberta Lucchese, Dario Di Stasio, Fedora Della Vella, Francesca Cura, Annalisa Palmieri, and Francesco Carinci. "Molecular Aspects of Drug-Induced Gingival Overgrowth: An In Vitro Study on Amlodipine and Gingival Fibroblasts." International Journal of Molecular Sciences 20, no. 8 (April 25, 2019): 2047. http://dx.doi.org/10.3390/ijms20082047.

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Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the fibrotic response, we investigated its effects on treated gingival fibroblast gene expression as compared with untreated cells. Materials and Methods: Fibroblasts from ATCC® Cell Lines were incubated with amlodipine. The gene expression levels of 12 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway was investigated in treated fibroblasts cell culture, as compared with untreated cells, by real time PCR. Results: Most of the significant genes were up-regulated. (CTNND2, COL4A1, ITGA2, ITGA7, MMP10, MMP11, MMP12, MMP26) except for COL7A1, LAMB1, MMP8, and MMP16, which were down-regulated. Conclusion: These results seem to demonstrate that amlodipine has an effect on the extracellular matrix of gingival fibroblast. In the future, it would be interesting to understand the possible effect of the drug on fibroblasts of patients with amlodipine-induced gingival hyperplasia.
2

Liu, Kaining, Bing Han, Jianxia Hou, Jianyun Zhang, Jing Su, and Huanxin Meng. "Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo." PeerJ 9 (January 4, 2021): e10279. http://dx.doi.org/10.7717/peerj.10279.

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Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Results Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Conclusions Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.
3

Smith, Q. T., and J. E. Hinrichs. "Phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not Alter the Effects of Bacterial and Amplified Plaque Extracts on Cultures of Fibroblasts from Normal and Overgrown Gingivae." Journal of Dental Research 66, no. 8 (August 1987): 1393–98. http://dx.doi.org/10.1177/00220345870660082201.

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Local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (PHT) therapy. Variability in the cytotoxicity of plaque components or of plaque substances plus PHT and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. Fibroblasts from healthy and overgrown gingivae were incubated with (a) PHT and its major human metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), (b) microbial and "amplified" plaque extracts, and (c) microbial and "amplified" plaque extracts plus PHT and HPPH. Cell numbers and cell-associated protein were determined for each incubation preparation. A wide range in cytotoxic response to a particular microbial or plaque extract occurred among cell strains. Plaque extracts from different subjects had variable cytotoxicity toward a cell strain. The differences among fibroblast strains in response to an extract and the variability in cytotoxicity of different plaque extracts toward a cell strain were not related to their source from normal or overgrown gingivae. Cell numbers and cell-associated protein were similar for incubation mixtures containing extracts with and without PHT and HPPH. These data do not show differences among cytotoxicity levels of plaque extracts, the response of particular gingival fibroblast strains to plaque components, or interaction between drugs and certain plaque samples which explain development of gingival overgrowth in some subjects receiving chronic PHT therapy.
4

Francetti, Luca, Claudia Dellavia, Stefano Corbella, Nicolò Cavalli, Claudia Moscheni, Elena Canciani, and Nicoletta Gagliano. "Morphological and Molecular Characterization of Human Gingival Tissue Overlying Multiple Oral Exostoses." Case Reports in Dentistry 2019 (May 22, 2019): 1–10. http://dx.doi.org/10.1155/2019/3231759.

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Gingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronic traumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues in genetically predisposed subjects. Since an imbalance in collagen turnover plays a key role in the development of gingival overgrowth leading to an accumulation of collagen in gingival connective tissue, in this study we described the histological and molecular features of three oral overgrowths obtained from a 34-year-old woman previously operated for FGG in order to evaluate a possible relationship between exostoses and overgrown tissue. Healthy and overgrown gingiva were analyzed by histological methods, and the expression of genes and proteins involved in collagen synthesis, maturation, and degradation was assessed in cultured fibroblasts obtained from gingival fragments at the molecular level. Our results show that general morphology and collagen content were similar in healthy and overgrown gingivae. However, fibroblasts obtained from the overgrown gingiva revealed an anabolic phenotype characterized by an increased collagen turnover and maturation. These findings indicate that an exostosis could act as a mechanical stimulus stretching the overlying connective tissue and triggering an anabolic phenotype of gingival fibroblasts and suggest to use minimally invasive surgical techniques to avoid traumatizing the periosteal tissues for the eradication of the exostosis with minimal relapses.
5

Danastri, Arifia Anindita, Suryono Suryono, and Kwartarini Murdiastuti. "THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION." ODONTO : Dental Journal 8, no. 2 (December 22, 2021): 25. http://dx.doi.org/10.30659/odj.8.2.25-31.

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ABSTRACTBackground: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.
6

Lauritano, Dorina, Marcella Martinelli, Alessandro Baj, Giada Beltramini, Valentina Candotto, Francesco Ruggiero, and Annalisa Palmieri. "Drug-induced gingival hyperplasia: An in vitro study using amlodipine and human gingival fibroblasts." International Journal of Immunopathology and Pharmacology 33 (January 2019): 205873841982774. http://dx.doi.org/10.1177/2058738419827746.

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Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 ( CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.
7

Walters, J. D., R. J. Nakkula, and P. Maney. "Modulation of Gingival Fibroblast Minocycline Accumulation by Biological Mediators." Journal of Dental Research 84, no. 4 (April 2005): 320–23. http://dx.doi.org/10.1177/154405910508400405.

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Gingival fibroblasts actively accumulate tetracyclines, thereby enhancing their redistribution from blood to gingiva. Since growth factors and pro-inflammatory cytokines regulate many fibroblast activities, they could potentially enhance fibroblast minocycline accumulation. To test this hypothesis, we treated gingival fibroblast monolayers for 1 or 6 hours with platelet-derived growth factor-BB (PDGF), fibroblast growth factor-2 (FGF), transforming growth factor-β1 (TGF), or tumor necrosis factor-α (TNF). Minocycline uptake was assayed at 37° by a fluorescence method. All 4 factors significantly enhanced minocycline uptake (P ≤ 0.008, ANOVA), primarily by increasing the affinity of transport. Treatment for 6 hours with 10 ng/mL FGF, PDGF, TGF, or TNF enhanced fibroblast minocycline uptake by 19% to 25%. Phorbol myristate acetate enhanced fibroblast minocycline uptake by 28%, suggesting that protein kinase C plays a role in up-regulating transport. These effects on transport provide a mechanism by which systemic tetracyclines could be preferentially distributed to gingival wound or inflammatory sites.
8

Ruggeri, A., L. Montebugnoli, A. Matteucci, N. Zini, L. Solimando, D. Servidio, P. Suppa, M. Cadenaro, L. Cocco, and L. Breschi. "Cyclosporin A Specifically Affects Nuclear PLCβ1 in Immunodepressed Heart Transplant Patients with Gingival Overgrowth." Journal of Dental Research 84, no. 8 (August 2005): 747–51. http://dx.doi.org/10.1177/154405910508400812.

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One of the most commonly observed adverse effects of cyclosporin A (CsA) is the development of gingival overgrowth (GO). Fibroblasts are involved in GO, but the question why only a percentage of patients undergoing CsA treatment shows this side-effect remains unanswered. In a previous study, CsA has been demonstrated to induce over-expression of phospholipase C (PLC) β1 in fibroblasts of patients with clinical GO, in cells from both enlarged and clinically healthy gingival sites. In this work, we assessed the expression of PLCβ isoforms to investigate whether the exaggerated fibroblast response to CsA related to increased PLCβ1 expression could also be detected in CsA-treated patients without clinical signs of GO. Our results support the hypothesis of a multi-factorial origin of gingival overgrowth, including specific changes within the gingival tissues orchestrating fibroblastic hyper-responsiveness as a consequence of a long-term in vivo exposure to cyclosporin A.
9

Damanaki, Anna, Marjan Nokhbehsaim, Sigrun Eick, Werner Götz, Jochen Winter, Gerhard Wahl, Andreas Jäger, Søren Jepsen, and James Deschner. "Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies." Mediators of Inflammation 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/912821.

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Adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), are molecules, which are produced in adipose tissue. Recent studies suggest that NAMPT might also be produced in the tooth-supporting tissues, that is, periodontium, which also includes the gingiva. The aim of this study was to examine if and under what conditions NAMPT is produced in gingival fibroblasts and biopsies from healthy and inflamed gingiva. Gingival fibroblasts produced constitutively NAMPT, and this synthesis was significantly increased by interleukin-1βand the oral bacteriaP. gingivalisandF. nucleatum. Inhibition of the MEK1/2 and NFκB pathways abrogated the stimulatory effects ofF. nucleatumon NAMPT. Furthermore, the expression and protein levels of NAMPT were significantly enhanced in gingival biopsies from patients with periodontitis, a chronic inflammatory infectious disease of the periodontium, as compared to gingiva from periodontally healthy individuals. In summary, the present study provides original evidence that gingival fibroblasts produce NAMPT and that this synthesis is increased under inflammatory and infectious conditions. Local synthesis of NAMPT in the inflamed gingiva may contribute to the enhanced gingival and serum levels of NAMPT, as observed in periodontitis patients. Moreover, local production of NAMPT by gingival fibroblasts may represent a possible mechanism whereby periodontitis may impact on systemic diseases.
10

Kujundzic, Bojan, Zlatibor Andjelkovic, Radmila Maric, Ruzica Lukic, Veljko Maric, Helena Maric, Miroslav Obrenovic, and Sinisa Kojic. "Periodontal Disease: Correlation with Histological and Immunological Parameters." Experimental and Applied Biomedical Research (EABR) 24, no. 1 (March 1, 2023): 57–62. http://dx.doi.org/10.2478/sjecr-2020-0024.

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Abstract Periodontal disease is inflammatory pathological conditions in the gingiva and dental support structures that usually results in extracellular matrix and connective tissue destruction. During periodontitis, inflammatory cells facilitate collagen and connective tissue loss, affects the number and activity of fibroblasts and its production of local collagen networks. Aim of this study was to evaluate collagen density and accumulation of collagen producing fibroblast and macrophages in affected tissue of periodontal disease. Histological and immunohistochemical analyzes were performed on paraffin embedded tissue sections of gingival biopsies, obtained from 30 patients with diagnosis of periodontal disease and 10 healthy donors. Tissue sections of gingival of patients with periodontal disease had significantly decreased collagen volume density and visible fragmentation and lysis of the collagen fibers, decreased number of fibroblasts, accompanied with increased accumulation of macrophages. Presented data implicate that macrophages accumulation may be the cause of enzyme mediated collagen destruction
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Journal articles Dissertations / Theses

Dissertations / Theses on the topic "Fibroblastes gingivaux":

1

Lorimier, Sandrine. "L'influence de l'environnement cellulaire sur le phenotype des fibroblastes gingivaux et dermiques." Paris 5, 1996. http://www.theses.fr/1996PA05M091.

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2

Senni, Karim. "Effets de polysaccharides d'origine marine sur le remodelage des tissus gingivaux et dermiques." Paris 13, 2000. http://www.theses.fr/2000PA132031.

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Le remodelage du tissu conjonctif est une phase essentielle à certains phénomènes physiologiques tels que la cicatrisation et les développements embryonnaire et post-natal. Un remodelage matriciel harmonieux nécessite un équilibre finement contrôlé entre synthèses et dégradations macromoléculaires. Toute altération de ces phénomènes conduit à des situations pathologiques ; ainsi une sur-expression des macromolécules matricielles aboutit à diverses fibroses tandis que les pathologies inflammatoires chroniques (parodontites, arthrose, emphysème) impliquent une dérégulation du potentiel protéolytique tissulaire. Les polysaccharides sulfatés tels que l'héparine, les héparanes sulfate, les dextranes fonctionnalisés ainsi que les fucanes ont de nombreux effets biologiques. Ces molécules sont capables d'agir sur le cycle cellulaire, d'influencer la synthèse de certaines macromolècules matricielles tels que les collagènes ou encore de modifier l'expression et l'activation des métalloprotéases matricielles (MMPs). Le but de nos travaux a été d'étudier l'influence des fucanes, polysaccharides pariétaux d'algues brunes, sur certains paramètres impliqués dans le remodelage tissulaire. Ainsi, nous avons pu montrer que les fucanes étaient capables : d'inhiber la prolifération des fibroblastes gingivaux en culture et à l'inverse d'augmenter celles des fibroblastes dermiques ; d'accélérer la fibrillation des collagènes dans des cultures fibroblastiques ; d'inhiber l'expression des MMPs par des fibroblastes dermiques tout en augmentant la complexation de ces protéases avec leurs inhibiteurs spécifiques (TIMPs) ; d'inhiber directement l'élastase leucocytaire tout en protégeant les fibres élastiques dermiques de l'action de cette sérine protéase. Ce travail qui a fait l'objet d'un dépôt de brevet (n°W09932099) pourrait aboutir au développement de nouveaux composés à partir d'algues brunes capables d'inhiber les effets délétères engendrés par les destructions tissulaires occasionnées lors de pathologies chroniques.
3

Naveau, Adrien. "Traitement de l'anévrysme abdominal aortique par transplantation de fibroblastes gingivaux autologues : études in vitro." Paris 5, 2007. http://www.theses.fr/2007PA05M006.

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La formation de l’anévrisme abdominal aortique (AAA) est associée à une dégradation matricielle et une augmentation de l’activité des métalloprotéases. Nous avons voulu tester les propriétés cicatricielles quasi embryonnaires attribuées aux fibroblastes gingivaux (GF) sur ces artères ex vivo. Pour identifier les GF en cocuture, nous avons d’abord mis au point une méthodologie de marquage à partir de nanoparticules anioniques. Nous avons ensuite analysé les sécrétions de métalloprotéases (MMP)-9, MMP-1 et MMP-3 et de leur inhibiteur tissulaire (TIMP)-1, dans les cocultures de fragments d’aorte en présence de GF sur 21 jours, ainsi que dans des cocultures de cellules musculaires lisses avec des fibroblastes gingivaux, dermiques et adventitiels. Ces résultats in vitro montrent une augmentation de TIMP-1 en présence des GF qui inhibe les enzymes et préserve notamment le capital réseau élastique artériel. La transplantation de GF nous parait une approche intéressante pour traiter les AAA
Abdominal aortic aneurysm (AAA) formation is associated with matrix degradation an metalloproteinases activity increase. We aimed to test the embryo-like healing propertie attributed to the gingival fibroblast (GF) on these arteries ex vivo. In order to identify the GF in coculture, we first established a labeling method from anioni nanoparticles. We have then analyzed the secretion of metalloproteinases (MMP)-9, MMP- and MMP-3, and their tissue inhibitor (TIMP)-1 in rabbit aortic rings cultured in presence o GF, and in smooth muscle cells cultured gingival, dermal and adventitial fibroblasts. These in vitro resuits do show an increase of TIMP-1 associated with GF presence, whic inhibits these enzymes and protects as well the arterial elastic network. The GF transplantation seems to us of crucial interest to treat AAA
4

Letzelter, Corinne. "Cytotoxicite activites proteolytiques des produits metaboliques de bacteries buccales vis-a-vis de fibroblastes gingivaux in vitro." Toulouse 3, 1999. http://www.theses.fr/1999TOU30092.

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Le but de notre travail a ete d'evaluer la cytotoxicite et l'activite proteolytique des toxines liberees dans leur milieu de culture (milieu schaedler) par 5 souches de bacteries frequemment rencontrees dans l'endodonte infecte : prevotella nigrescens, peptostreptococcus micros, capnocytophaga ochracea, fusobacterium necrophorum et fusobacterium russii et 2 souches qui en sont generalement absentes : fusobacterium nucleatum polymorphum et actinobacillus actinomycetemcomitans. Les surnageants de culture de ces bacteries, dilues au 1/10 dans le milieu de culture des fibroblastes (mem + 10%svf), entrainent des inhibitions de proliferation des fibroblastes gingivaux dont l'intensite peut etre classee comme suit : 10 a 20% pour p. Nigrescens, c. Ochracea et p. Micros, 100% pour les 3 souches de fusobacterium. Le surnageant le plus toxique est celui de a. Actinomycetemcomitans (100% d'inhibition pour une dilution au 1/1000). L'activite proteolytique des surnageants bacteriens vis a vis de la fibronectine et du collagene i, principales macromolecules constituant la matrice extracellulaire des cultures de fibroblastes a confluence, a ete recherchee par immunofluorescence indirecte. Aucune activite proteolytique n'a ete detectee dans les surnageants d'a. Actinomycetemcomitans, p. Micros et c. Ochracea. Les surnageants de p. Nigrescens et f. Necrophorum ont entraine une lyse incomplete des fibrilles de collagene i. Seul l'inhibiteur de protease, pmsf, a partiellement inhibe l'activite collagenolytique de ces surnageants, suggerant la participation d'une serine protease. Une metalloprotease telle que l'elastase n'est pas impliquee et la presence de collagenase n'a pu etre demontree. Une degradation totale du reseau de fibronectine a ete observee avec les surnageants de p. Nigrescens et f. Necrophorum, elle etait plus moderee avec les 2 autres souches de fusobacterium. Ces activites proteolytique ont ete totalement inhibee par la chaleur, le svf et +/- partiellement par le pmsf selon les souches bacteriennes. La leupeptine, l'aprotinine, la pepstatine et l'inhibiteur de trypsine du soja n'ont aucun effet. L'immunodetection sur western blot des produits de proteolyse de la fibronectine a montre que les enzymes de p. Nigrescens et f. Necrophorum entrainaient la disparition des dimeres de 440 kda et l'apparition de fragments de proteolyse de 180 kda pour p. Nigrescens, 120 kda pour f. Nucleatum polymorphum et de 70 a 210 kda pour f. Necrophorum. L'ensemble des resultats est en faveur de la presence de proteases differentes dans les divers surnageants bacteriens etudies. Tous les surnageants bacteriens ont induit une chute de l'activite de la phosphatase alcaline des fibroblastes gingivaux (differencies en preosteoblastes) et une augmentation de l'activite de la phosphatase acide.
5

Giraud, Andréas. "Développement d’une approche de thérapie cellulaire de l’anévrisme de l’aorte abdominale utilisant les fibroblastes gingivaux chez la souris." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB029/document.

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L’anévrisme de l’aorte abdominale (AAA) correspond à une dilatation progressive de l’aorte dont la complication principale, et potentiellement mortelle, est la rupture. La physiopathologie de l’AAA est complexe mais elle comporte une destruction des fibres élastiques liée à l’activité des métalloprotéinases (MMPs) matricielles, une apoptose des cellules musculaires lisses et une infiltration inflammatoire chronique. Actuellement, il n’existe aucun traitement pharmacologique permettant de limiter la progression et la rupture de l’AAA. Les fibroblastes gingivaux (FGs) sont des cellules multipotentes anti-inflammatoires qui permettent une réparation parfaite de la gencive sans cicatrice ni fibrose. Ces propriétés font des FGs un candidat potentiel pour une approche de thérapie cellulaire de l’AAA. Les FGs murins cultivés in vitro produisent une grande quantité de TIMP 1, un inhibiteur des MMPs. Lorsque les FGs sont implantés autour de l’aorte, ils survivent, prolifèrent et s’organisent en une épaisse couche cellulaire au niveau de l’adventice. In vivo, les FGs produisent au niveau de la paroi aortique de l’IL-10, du TGF-β, du collagène et du TIMP-1. Parallèlement les FGs inhibent l’activité protéolytique de la MMP-9. Dans un modèle d’AAA induit par l’élastase, les FGs inhibent la dégradation de l’élastine, l’infiltration macrophagique et lymphocytaire ainsi que la croissance des AAA. L’invalidation génétique de TIMP-1 dans les FG abolit leur effet protecteur sur le remodelage aortique et la formation des AAA. Dans un second modèle d’AAA induit par l’infusion d’angiotensine II et l’injection d’anticorps neutralisant anti-TGF-β, les FGs limitent localement le développement de la maladie vasculaire et préviennent la rupture de l’aorte abdominale. L’ensemble de ces données expérimentales suggère qu’une approche de thérapie cellulaire utilisant les FG pourrait permettre de ralentir la croissance de l’AAA et in fine sa rupture
Abdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinases-mediated extracellular matrix destruction. Depiste improvement in the understanding of the pathophysiology of the aortic aneurysm disease, no pharmacological treatment is available to limit dilatation and/or rupture. In the study reported here, we tested whether periadventitial allograft of GF prevented abdominal aortic aneurysmal growth and rupture in mice and investigated the mechanisms of vascular protection. In vitro, mouse GF proliferated and produced large amounts of anti-inflammatory cytokines and Timp-1, an inhibitor of metalloproteinases. When layed down in the periadventitial abdominal aorta, we documented that GF survived in vivo, proliferated and organized as a thick layer. Furthermore, GF locally produced Il-10, TGF-β and Timp-1. In an elastase-induced AAA, GF prevented both macrophage and lymphocyte infiltration, elastin degradation and aneurysm growth. Specific invalidation of Timp-1 in GF abolished the beneficial effect of cell therapy. In an Angiotensin II/anti-TGF-β model of AAA, GF cell therapy limited AAA development and prevented abdominal rupture. Gingival fibroblast is a promising cell therapy approach to inhibit aneurysmal progression and rupture through the local production of Timp-1
6

Soheili-Majd, Esmat. "Etudes des mécanismes de la cytotoxicité des biomatériaux dentaires sur les fibroblastes pulpaires et gingivaux humains : effets des anti-oxydants." Paris 5, 2004. http://www.theses.fr/2004PA05M122.

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La toxicité de dix biomatériaux d'obturation dentaire, non amalgame est étudiée et comparée sur les fibroblastes gingivaux et pulpaires humains : Ces biomatériaux sont toxiques in vitro. Les céments verre ionomères sont plus toxiques que les composites. Leur éluats diminuent le taux de glutathion intracellulaire (GSH). Certains antioxydants comme la NAC, le glutathion-ester et Trolox inhibent la déplétion du GSH et protègent contre ces effets toxiques. La vitamine C augmente la déplétion du GSH et la toxicité des éluats à base de CVI contenant des traces de fer. Le stress oxydatif pourrait constituer un des mécanismes essentiels de la toxicité des biomatériaux dentaires.
7

Kut-Lasserre, Christelle. "Effet protecteur des insaponifiables d'huile d'avocat et de soja sur la matrice conjonctive gingivale et sur leur capacite a remodeler cette matrice par les fibroblastes gingivaux humains en culture : etude ex vivo et in vitro." Paris 5, 1999. http://www.theses.fr/1999PA05M083.

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Papa, Steve. "Evaluation de l'adhérence gingivale et du potentiel antibactérien de surfaces structurées par laser femtoseconde pour l'implantologie orale." Electronic Thesis or Diss., Saint-Etienne, 2023. http://www.theses.fr/2023STET0063.

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Cette thèse traite des problèmes d'infections des implants dentaires, telles que la péri-implantite, causées par l'adhérence de bactéries parodontopathogènes. Elle explore l'utilisation de la texturation laser femtoseconde (fs-L) pour améliorer les propriétés des surfaces d'implants en alliage de titane (Ti6Al4V).Le projet, financé par l'ANR et mené en collaboration avec divers laboratoires, a utilisé des techniques de caractérisation avancées pour analyser les surfaces texturées et évaluer leur efficacité en conditions biologiques. Les résultats montrent que la texturation par fs-L permet de créer des structures de surface périodiques (LIPSS) micro et nanométriques, modifiant la surface de contact et par conséquent l’adhérence cellulaire et bactérienne. Les surfaces ainsi texturées ont démontré une réduction significative de l'adhérence des bactéries parodontopathogènes, telles que Porphyromonas gingivalis, réduisant ainsi potentiellement les risques de péri-implantite.Les études in vitro ont également confirmé une meilleure adhérence des fibroblastes gingivaux sur les surfaces texturées, ce qui pourrait réduire les risques d’infiltration bactérienne et ainsi améliorer la stabilité et l’intégration des implants.En conclusion, la texturation laser femtoseconde des surfaces d'implants dentaires est prometteuse pour créer des implants plus durables et doublement fonctionnalisés, améliorant l'adhérence cellulaire et possédant des propriétés antibactériennes accrues. Ces avancées ouvrent la voie à des implants répondant mieux aux défis cliniques actuels tout en contribuant à la lutte contre l'antibiorésistance. Des études supplémentaires plus proches d'une situation clinique sont prévues pour valider ces résultats et explorer les interactions entre les topographies fs-L et les réponses biologiques des tissus environnants
This thesis addresses issues related to infections of dental implants, such as peri-implantitis, caused by the adhesion of periodontopathogenic bacteria. It explores the use of femtosecond laser (fs-L) texturing to enhance the properties of titanium alloy (Ti6Al4V) implant surfaces.The project, funded by the ANR and conducted in collaboration with various laboratories, employed advanced characterization techniques to analyze textured surfaces and evaluate their effectiveness under biological conditions. The results demonstrate that fs-L texturing can create micro and nanometric periodic surface structures (LIPSS), altering the contact surface and thus cellular and bacterial adhesion. Textured surfaces showed a significant reduction in the adhesion of periodontopathogenic bacteria, such as Porphyromonas gingivalis, potentially reducing the risks of peri-implantitis.In vitro studies also confirmed better adhesion of gingival fibroblasts to textured surfaces, which could reduce the risk of bacterial infiltration and thus improve implant stability and integration.In conclusion, femtosecond laser texturing of dental implant surfaces holds promise for creating more durable and dual-functionalized implants, enhancing cellular adhesion, and possessing increased antibacterial properties. These advancements pave the way for implants better suited to current clinical challenges while contributing to the fight against antibiotic resistance. Further studies closer to clinical settings are planned to validate these results and explore the interactions between fs-L topographies and the biological responses of surrounding tissues
9

Azevedo, Fabiola Pontes. "Avaliação comparativa do comportamento adaptativo de fibroblastos humanos cultivados de mucosa palatina não marginal e de enxerto gengival em área marginal." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-05062013-093746/.

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Enxertos gengivais livres são importantes para garantir condições necessárias para o estabelecimento da homeostasia do periodonto de proteção. O processo de inflamação não ocorre por igual em todos os tecidos conjuntivos do organismo e os fibroblastos têm a capacidade de reagir a estímulos agressivos por meio de liberação de diversas citocinas, que desempenham importante função na formação do infiltrado inflamatório. Até o presente trabalho, não há relatos na literatura acerca da comparação do comportamento dos fibroblastos que compõem a mucosa palatina não marginal e dos fibroblastos provenientes de enxerto gengival livre (EGL) marginal em resistir aos estímulos agressores que ocorrem na doença periodontal. Dessa forma, a proposta do presente trabalho foi investigar se os fibroblastos da mucosa palatina não marginal mudariam seu perfil de secreção de citocinas quando enxertados na margem gengival. Foram coletadas biópsias da mucosa palatina no momento da cirurgia de EGL (período inicial) e após 4 meses (período final) no momento da cirurgia para recobrimento radicular. Os fibroblastos foram cultivados e estimulados com LPS de Porphyromonas gingivalis (Pg) e de Escherichia coli (Ec) por 24h e 48h para avaliação comparativa da expressão de citocinas e mediadores do reparo tecidual, como: IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF e CXCL16. As citocinas foram quantificadas no sobrenadante das células por meio de ensaio imunoenzimático (ELISA). Para a citocina IL-6, os fibroblastos da mucosa palatina não marginal mantiveram o mesmo perfil de secreção quando enxertados na área gengival marginal; para MIP-1α a secreção se mostrou aumentada de forma estatisticamente significativa pelos fibroblastos obtidos do enxerto gengival marginal após 48h de estímulo por Pg em comparação com os fibroblastos da área palatina não marginal; a secreção de IL-8 pelos fibroblastos da mucosa palatina não marginal foi maior em resposta ao desafio por LPS de Pg e os fibroblastos obtidos do enxerto gengival marginal exibiram secreção até mesmo sem o estímulo de LPS; apenas os fibroblastos do enxerto gengival marginal apresentaram secreção de TGF-β, mesmo na ausência de estímulo por LPS; a secreção de VEGF e CXCL16 não foi detectada pelos fibroblastos analisados. Conclui-se que os fibroblastos provenientes de uma mucosa palatina não marginal parecem se adaptar às condições locais quando enxertados na área gengival marginal, oferecendo evidência de sua participação efetiva na produção de mediadores inflamatórios importantes para o processo de homeostasia do periodonto marginal.
Free gingival grafts are important to ensure conditions for the establishment of homeostasis of the periodontal soft tissues. The process of inflammation does not occur the same way in all connective tissues and fibroblasts have the ability to respond to aggressive stimuli through the release of various cytokines, which play an important role in the inflammatory infiltrate formation. In literature, there are no studies comparing the behavior of fibroblasts from palatal mucosa (not marginal) and fibroblasts from marginal free gingival graft (FGG) regarding their resistance towards periodontal disease aggressive stimuli. Thus, the purpose of this study was to investigate whether fibroblasts from the palatal mucosa behave differently when grafted to the gingival margin considering their mechanism of cytokine secretion. Biopsies from the palatal mucosa were collected at the time of FGG surgery (initial period) and after 4 months (final period) when surgery for root coverage was performed. The fibroblasts were cultured and stimulated with LPS of Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) for 24 and 48 hours in order to make a comparative evaluation of cytokines and mediators of tissue repair expression, such as IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF and CXCL16. Cytokines were measured in the cell supernatant by enzyme immunoassay (ELISA). For cytokine IL- 6, fibroblasts from palatal mucosa maintained the same secretion pattern when grafted to the gingival margin; for MIP-1α the secretion was significantly increased by fibroblasts from the marginal gingival graft after 48 hours of stimulation with Pg when compared to palatal mucosa fibroblasts; IL-8 secretion by palatal mucosa fibroblasts did not increase in response to Pg LPS challenge and fibroblasts from marginal gingival graft showed secretion even without the stimulus of LPS; only fibroblasts from marginal gingival graft showed secretion of TGF-β, even in the absence of LPS stimulation; VEGF and CXCL16 secretion by fibroblasts was not detected. It was concluded that fibroblasts from palatal mucosa seem to adapt to local conditions when grafted to the gingival margin area, providing evidence of its effective participation in the homeostasis of marginal periodontium through the production of important inflammatory mediators.
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Kreidly, Mariam. "IL-34 Expression in Gingival Fibroblasts, Gingival Crevicular Fluid and Gingival Tissue." Thesis, Umeå universitet, Tandläkarutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-97861.

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IL-34 is a protein associated with bone degenerative diseases but the role in periodontal disease is unknown. The aim of this study was to assess the expression of IL-34 in primary human gingival fibroblasts (GF) and investigate if the expression is regulated by the pro-inflammatory cytokines interleukin-1 (IL-1β) and tumor necrosis factor α(TNF-α). We also investigated if IL-34 is detectible in gingival crevicular fluid (GCF) in healthy, gingivitis and periodontitis sites. Furthermore, we examined if healthy and inflamed gingival tissue contains IL-34. GF were stimulated by IL-1β300 pg/ml and TNF-α10 ng/ml. IL-34 mRNA was measured by quantitative real-time PCR (qPCR). GCF was collected from 11 healthy, 10 gingivitis, and 21 periodontitis gingival crevices. IL-34 protein was quantified using enzyme-linked immunoabsorbent assays (ELISA). Healthy and inflamed gingival tissue biopsies were collected and examined using immunohistochemistry (IHC). IL-34 mRNA was expressed in GF and the expression was enhanced 12x fold-change versus control by TNF-α10 ng/ml and 4x fold-change versus control by IL-1β300 pg/ml. IL-34 was also present in GCF but no significant difference in IL-34 protein was detected between the healthy, gingivitis, and periodontitis groups. Healthy and inflamed gingival tissue showed equal amounts of IL-34 protein in the epithelium while sub-epithelially the inflamed tissue showed higher levels of IL-34 protein. Pro-inflammatory cytokines stimulate IL-34 mRNA expression in GF. IL-34 protein is present in GCF and gingival tissue which demands further investigation about the eventual role of IL-34 in the pathogenesis of periodontitis.
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Books on the topic "Fibroblastes gingivaux":

1

Chou, Debra Hsin-I. TNF-r̀egulation of phagocytosis in human gingival fibroblasts. Ottawa: National Library of Canada, 1995.

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Chou, Debra Hsin-I. TNF-[alpha] regulation of phagocytosis in human gingival fibroblasts. [Toronto: Faculty of Dentistry, University of Toronto], 1995.

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Németh, Endre. Responses of gingival fibroblast and endothelial cell populations to experimentally-inducedinflammation in monkeys. Ottawa: National Library of Canada, 1993.

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Yang, Po Fong. Filamentous actin disruption and diminished inositol phosphate response in gingival fibroblasts caused by Treponema denticola. [Toronto: University of Toronto, Faculty of Dentistry], 1998.

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Németh, Endre. Responses of gingival fibroblast and endothelial cell populations to experimentally-induced inflammation in monkeys. [Toronto: Faculty of Dentistry, University of Toronto], 1993.

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Battikhi, Tulin. Treponema Denticola outer membrane extract enhances the phagocytosis of collagen coated-beads by human gingival fibroblasts. [Toronto: University of Toronto, Faculty of Dentistry], 1998.

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Book chapters on the topic "Fibroblastes gingivaux":

1

Egusa, Hiroshi. "The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry." In Interface Oral Health Science 2011, 9–13. Tokyo: Springer Japan, 2012. http://dx.doi.org/10.1007/978-4-431-54070-0_2.

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Choi, C. H., B. I. Kim, H. K. Kwon, and Suck Jin Hong. "Effects of Herbal Extracts on Dental Plaque Formation and Human Gingival Fibroblasts." In Advanced Biomaterials VII, 773–76. Stafa: Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-436-7.773.

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Chou, Patty, and Trudy J. Milne. "Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts." In Methods in Molecular Biology, 373–83. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-820-1_23.

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Kerr, Janet S., George A. Boswell, Neil R. Ackerman, and Theresa M. Stevens. "Induction of Superoxide Dismutase Activity by Paraquat or Edu in Human Gingival Fibroblasts." In Oxygen Radicals in Biology and Medicine, 695–98. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5568-7_109.

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Khashaba, Rania M. "Calcium Phosphate based Perforation Repair Materials using Human Gingival Fibroblasts: An in vitro Cytotoxicity Evaluation." In New Horizons in Medicine and Medical Research Vol. 9, 83–91. Book Publisher International (a part of SCIENCEDOMAIN International), 2022. http://dx.doi.org/10.9734/bpi/nhmmr/v9/15713d.

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El-Bialy, Tarek, Hagai Hazan Molina, Yuval Aizenbud, Wasif Qayyum, Saleem Ali, and Dror Aizenbud. "Effects of Intraligamentary Injection of Osteogenic-Induced Gingival Fibroblasts on Cementum Thickness in the Dog Model of Tooth Root Resorption." In Advances in Experimental Medicine and Biology. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/5584_2020_551.

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Conference papers on the topic "Fibroblastes gingivaux":

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Vo Quang Costantini, S., S. Petit, A. Nassif, F. Ferre, and B. Fournier. "Perspectives thérapeutiques du matrisome gingival dans la cicatrisation pathologique." In 66ème Congrès de la SFCO. Les Ulis, France: EDP Sciences, 2020. http://dx.doi.org/10.1051/sfco/20206602013.

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Introduction : La muqueuse gingivale présente un potentiel de cicatrisation parmi les plus efficaces des tissus humains adultes. Le fibroblaste gingival joue un rôle primordial dans ces phénomènes de cicatrisation par ses interactions avec les éléments de la matrice extra-cellulaire (MEC). La peau, dont les capacités de cicatrisation sont moindres que la gencive, constitue un tissu de comparaison idéal pour étudier les phénomènes de cicatrisation. Ce travail propose de caractériser les différences entre gencive et peau à l’aide d’une cartographie des protéines de leur matrice extra-cellulaire respective et de leurs profils transcriptomiques. Matériels et méthodes : Les matrisomes et les profils d’expression génique (transcriptome) des fibroblastes gingivaux et dermiques ont été comparés après 14 jours de culture à confluence. Ces résultats ont été confirmés par l’analyse des bases de données publiques à l’aide d’outils bioinformatiques (GenePattern et GEO2R). Ils seront complétés par des modèles in vivo : deux prélèvements cutanés et gingivaux appariés chez trois souris saines (C57-Black 6) seront analysés comme précédemment. Résultats : Les résultats préliminaires montrent une surexpression des glycoprotéines dans la MEC des fibroblastes gingivaux. Ces dernières sont associées à la régulation de la synthèse collagéniques et élastiques. Ces résultats, confirmés par transcriptome, mettent en évidence une différence qualitative de la MEC synthétisée par ces deux types cellulaires. Discussion : Ces résultats montrent l’hétérogénéité du fibroblaste qui synthétise des MEC différentes en fonction de son origine tissulaire. Cette cartographie souligne la spécificité du tissu muqueux oral. Ces données préliminaires permettront d’identifier des protéines liées à la cicatrisation ad integrum de la muqueuse orale. Ces résultats pourraient ainsi orienter la fabrication de nouveaux supports en ingénierie tissulaire et présenter de nouvelles pistes thérapeutiques pour la cicatrisation cutanée pathologique
2

Lafont, J., J. H. Catherine, M. Lejeune, U. Ordioni, R. Lan, and F. Campana. "Manifestations buccales de la sclérose tubéreuse de Bourneville." In 66ème Congrès de la SFCO. Les Ulis, France: EDP Sciences, 2020. http://dx.doi.org/10.1051/sfco/20206603014.

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L’objectif de ce travail est de faire le point sur les manifestations buccales de la sclérose tubéreuse de Bourneville (STB) à travers le cas d’un jeune patient. Un jeune homme de 15 ans était adressée pour la mise en place de minivis orthodontique afin de fermer des espaces d’agénésies de 35 et 45. L’interrogatoire retrouvait une STB dont les manifestations épileptiques étaient traitées par de la lamotrigine 75mg/j et de la carbamazépine LP 200mg/j. L’examen clinique exo-buccal retrouvait des macules hypochromiques sur le membre inférieur droit, des angiofibromes faciaux et une malformation vasculaire jugale gauche. L’examen endo-buccal retrouvait de multiples lésions buccales sur les papilles interdentaires pouvant évoquer des fibromes ou des hamartomes. Une biopsie était réalisée et retrouvait un revêtement malpighien, discrètement hyperplasique et sans atypie cellulaire. Les faisceaux collagènes du conjonctif étaient mêlés à de nombreux fibroblastes aux noyaux réguliers, sans mitose visible. Les cellules inflammatoires, essentiellement mononuclées, étaient dispersées mais tendaient à se regrouper autour de vaisseaux nombreux et hyperplasiques. L’examen concluait à un fibrome. Aucun traitement buccal n’était proposé devant l’absence de symptôme et de demande esthétique. La STB est une maladie génétique autosomique dominante avec une incidence de 1/10 000. Elle est liée à une mutation du gène TSC1 sur le chromosome 9 ou du gène TSC2 sur le chromosome 16 qui perturbe la sécrétion d’une protéine régulant la voie mTOR. C’est une maladie multisystème avec une expression clinique variable. Les principaux symptômes sont l’épilepsie, le retard mental et la présence d’adénomes sébacés, mais la maladie est associée à un polymorphisme clinique rendant le diagnostic difficile. La conférence de consensus de 2012 a ainsi défini des critères diagnostiques majeurs (lésions cutanées, oculaires, cérébrales, cardiaques, pulmonaires, rénales,..) et mineurs dont deux sont bucco-dentaires. Le diagnostic est retenu devant deux critères majeurs ou un critères majeur et deux critères mineurs. Les signes oraux sont la présence de trois ou plus puits d’émail et deux ou plus fibromes gingivaux. Les fibromes gingivaux atteindraient 50 à 70% des patients. La région antérieure maxillaire semble la plus touchée. L’exérèse est indiquée en cas de gêne esthétique ou de saignements associés. Actuellement, les inhibiteurs de mTOR représentent une option thérapeutique proposée dans la prise en charge des patients atteints de STB. La STB est une pathologie rare. La présence de lésions buccales fait partie des critères diagnostiques.
3

Thaweboon, Sroisiri, Ratchaporn Srichan, Supaporn Mala, and Boonyanit Thaweboon. "The Development of Artificial Saliva with Oral Wound Healing Property." In 2023 7th International Conference on Nanomaterials and Biomaterials & 2023 5th Asia Conference on Material and Manufacturing Technology. Switzerland: Trans Tech Publications Ltd, 2024. http://dx.doi.org/10.4028/p-wc6acn.

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Wound healing is a normal biological mechanism of the body that helps to maintain the integrity of the tissue. In this process, fibroblasts play an important role in supporting wound healing by migrating to the injury site and laying down a new extracellular matrix. Oral wounds heal more rapidly than skin wounds due to the presence of saliva. However, a reduced salivary flow rate or hyposalivation is frequently found in some patients due to their systemic conditions or intake of various medications. In order to control or treat hyposalivation, the use of artificial saliva is recommended for these patients. Various agents were added to artificial saliva to improve its properties. The aim of the present study was to investigate the effect of artificial saliva containing vanillin on the wound healing of human gingival fibroblasts by inducing cell migration in vitro. Human gingival fibroblasts isolated from human gingiva were purchased from Scien Cell Research Laboratories, USA. The migratory ability of fibroblasts was performed on a confluent monolayer by the wound healing scratch assay. Artificial saliva with different concentrations of vanillin (0.12% to 4% w/v) was added and incubated for 24 h. Artificial saliva without vanillin was used as a control. The migration cells were fixed with 25% methanol and 0.2% toluidine blue. In vitro cell migration to the wound area was determined by photographing with an inverted microscope coupled to a digital camera (Nikon D 5100). In the presence of 0.25%, 0.5%, and 1% w/v vanillin-containing artificial saliva, human gingival fibroblasts had a significantly higher potential to migrate into the wound area than a control (p-value <0.05). Data from this study provides the first scientific evidence to demonstrate the benefits of using artificial saliva containing vanillin to maintain healthy gums and accelerate oral wound healing. Rinsing the mouth with this artificial saliva is recommended as the most preferable method for moistening and lubricating the mouth and facilitating the healing of oral wounds in patients with hyposalivation.
4

Al Sufyani, Noha Moslah, Nahed Ahmed Hussien, and Yousef Mohammed Hawsawi. "Cytotoxic effect of synthesized silver nanoparticles on normal human gingival fibroblast GF01 cells." In 2021 International Conference of Women in Data Science at Taif University (WiDSTaif ). IEEE, 2021. http://dx.doi.org/10.1109/widstaif52235.2021.9430249.

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Almeida-Lopes, Luciana, Marcia M. M. Jaeger, Aldo Brugnera, Jr., and Josepa Rigau. "Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro." In BiOS '98 International Biomedical Optics Symposium, edited by John D. B. Featherstone, Peter Rechmann, and Daniel Fried. SPIE, 1998. http://dx.doi.org/10.1117/12.306022.

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Bakhori, Siti Khadijah Mohd, Shahrom Mahmud, Sam’an Malik Masudi, Azman Seeni, Dasmawati Mohamad, Ling Chuo Ann, and Amna Sirelkhatim. "Cytotoxicity evaluation of ZnO-eugenol (ZOE) using different ZnO structure on human gingival fibroblast." In PROCEEDING OF THE 3RD INTERNATIONAL CONFERENCE OF GLOBAL NETWORK FOR INNOVATIVE TECHNOLOGY 2016 (3RD IGNITE-2016): Advanced Materials for Innovative Technologies. Author(s), 2017. http://dx.doi.org/10.1063/1.4993327.

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7

Illeperuma, Rasika P., Young J. Park, Hwa K. Son, Jin M. Kim, Da-Woon Jung, Wanninayake M. Tilakaratne, and Jin Kim. "Abstract 5471: Implication of cytokines released from gingival fibroblasts exposed by areca nut extract in carcinogenesis." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-5471.

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8

Krismariono, A., N. Ulfa, U. N. Wardi, and S. C. S. Budijono. "Viability test of water hyacinth leaf extract (Eichornia Crassipes) on human gingival fibroblast cell culture." In THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0034976.

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9

Wasi, S., P. Alles, D. Gauthier, U. Bhargava, J. Farsi, J. E. Aubin, and J. Sodeki. "STUDIES ON SMALL MOLECULAR WEIGHT ADHESION PROTEINS (SAPs) FROM CONNECTIVE TISSUES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643556.

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We have identified a family of low molecular weight proteins with cell attachment properties in a variety of soft and mineralised connective tissues (Wong et al., Biochem. J. 232, 119, 1985). For further characterisation of these proteins we extracted porcine bones with 4 M guanidine hydrochloride and purified the proteins on a series of gel filtration columns The purifed SAPs comprise three bands with Mr -14 000 -17 000. All three proteins bound to heparin-sepahrose in both the presence and absence of 4M urea, and when eluted with 2 M NaCl they retained their cell binding capacity. These proteins promoted the adhesion and spreading of a variety of cell types, including normal fibroblasts, osteoblasts, and epithelial cells, and tumour (osteosarcoma) cells. On Western blotting SAPs did not cross-react with antibodies against fibronectin, laminin or type I collagen; however, they were recognised by a monoclonal antibody to human vitronectin, a polyclonal antibody to bovine vitronectin and polyclonal antibody to human somatomedin B. Dose response experiments indicated that maximum attachment of human gingival fibroblasts occurred in the presence or absence of fetal bovine serum on wells precoated with 2.5 μg/cm2 of SAPs. Attachment of cells to these proteins was partially inhibited by the synthetic pentapeptide Gly-Arg-Gly-Asp-Ser. Utilising the nitrocellulose cell binding assay of Hayman et al (J. Cell. Biol. 95, 20, 1982), the cell attachment to these proteins could be completely inhibited by heparin (100 units/mL) whereas up to 1000 units/mL of heparin had no inhibitory effect on cell attachment to fibronectin and vitronectin. The occurrence of these proteins in a variety of connective tissues and their recognition by different cell types may reflect their general biological role in adhesive mechanisms in both hard and soft connective tissues. Currently, we are investigating the relationship between SAPs and vitronectin, since it is possible that SAPs represent a tissue-processed form of vitronectin or may be novel attachment proteins with regions of homology with vitronectin
10

Wasi, S., S. Juodvalkis, P. Alles, and J. E. Aubin. "STUDIES ON THE DIRECT PROTEOLYTIC ACTION OF HUMAN TISSUE PLASMINOGEN ACTIVATOR ON HUMAN FIBRONECTIN AND VITRONECTIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644376.

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The ability of cells to make or break specific attachments to extracellular matrix (ECM) and other cells is important in cell migration, proliferation and wound repair. Specific attachment proteins believed to be involved in mediating these interactions comprise functional domains joined by protease sensitive segments. Proteases can conceivably modulate cellular interactions by releasing functional domains from parent molecules. Tissue plasminogen activator (t-pA) is known to participate in various pathophysiological processes. That t-pA may also act directly on structural proteins has not been investigated. We have studied the direct proteolytic action of melanoma t-pA on fibronectin (FN), vitronectin (VN) and laminin (LN). These were incubated with t-pA for 0 to 48 h in 50 mM Tris HCi, pH 7.4. The cleavage products were separated on polyacrylamide slab gels and blotted onto nitrocellulose strips. FN and VN fragments with cell attachment properties were identified by incubating the strips with human gingiva fibroblasts and staining with Amido black. Monoclonal antibodies to FN were used to identify heparin, cell and gelatin binding fragments. VN was converted to a major 55 Kd product as a function of time. Lower molecular weight species migrating at 45 Kd, 30 Kd and 15 Kd positions were also identified. Most of these fragments possessed cell attachment properties. LN became susceptible to t-pA digestion after dénaturation with H2O2. The catalytic activity of t-pA could be inhibited in the presence of nitrophenyl-p-guinidino benzoate (a synthetic inhibitor of plasminogen activator), whereas O-phenanthroline (a metalloprotease inhibitor), α 2-antiplasmin and trasylol had no effect. A monoclonal IgG preparation (HI 72 A1, kindly provided by Dr. David J. Loskutoff) that specifically inhibits t-pA also inhibited the protelyotic action of t-pA on FN. These data suggest that direct proteolytic action of t-pA on adhesive proteins may modulate cellular behaviour in various normal and pathological conditions which involve dynamic interactions between cells and ECM and where plasminogen activator levels are elevated either transiently or permanently, for example during tissue remodelling, wound-related repair and thrombolytic therapy.

Reports on the topic "Fibroblastes gingivaux":

1

J.P, Babu. Long-term dental adhesive toxicity on human gingival fibroblasts and epithelial cells. Science Repository, June 2019. http://dx.doi.org/10.31487/j.dobcr.2019.03.01.

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