Dissertations / Theses on the topic 'Fertility, Human Immunological aspects'
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1
Ramshaw, Anna Louise. "Immunological aspects of human atherosclerosis." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305549.
2
Norbeck, Oscar. "Clinical and immunological aspects of human parvovirus B19 infection /." Stockholm : Dept. of laboratory medicine, Karolinska institutet, 2005. http://diss.kib.ki.se/2005/91-7140-203-9/.
3
Clarke, Damian. "Essays on fertility and family size." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:94016283-a3dd-4b6a-8427-373b49a491be.
Abstract:
In these papers I discuss the causal estimation of the effects of fertility and fertility planning developments on mother and child outcomes. A number of concerns are raised with existing identification techniques, and alternative methodologies to consistently estimate the effect of interest are proposed. These concerns and new techniques are illustrated using microdata on slightly more than 43,000,000 births ocurring between 1972 and 2013. In the first substantive chapter (written with Sonia Bhalotra), we discuss the validity of the use of twin births in fertility research. We demonstrate that twin births are not random. Successfully taking twins to term depends upon positive maternal health behaviours and investments in the periods preceding birth. We show that this is of considerable concern for estimation techniques which rely on twin births being (conditionally) randomly assigned to identify causal effects. To illustrate, we consider the estimation of the child quantity-quality (QQ) trade-off, and show that existing instrumental variable estimates are inconsistent in the contexts examined. Upon partially correcting for the fact that twin births are not random, a statistically significant QQ trade-off begins to emerge. We close by examining a number of partial identification techniques to bound the true effect of fertility on child outcomes. In the second substantive chapter, I examine the effect of fertility control policies on the fertility decisions and outcomes of women. I consider the case of the emergency contraceptive pill in Chile. The staggered arrival of this technology to Chile over the last decade has resulted in the availability of the first safe and legal post-coital birth control policies. In a context of high teenage pregnancy rates, difference-in-difference (DD) style estimates suggest that this policy has accounted for reductions in short-term teen childbearing by as much as 7%, an effect similar to the arrival of abortion in the USA. This policy is also shown to reduce fetal deaths reported in early gestation with no similar reduction in late gestation: suggestive evidence that an alternative fertility control policy may reduce costly and dangerous illegal abortions. Finally, I turn to the use of DD estimators as a policy-analysis tool. I discuss how such estimators perform in the case of reforms which may not be sharply demarcated to treatment and control clusters, but rather subject to local spillovers or externalities. I propose an extension of the typical DD estimator: a spillover-robust DD estimator. This methodology is applied to estimate the effect of two localised fertility control reforms in Mexico and Chile, where women close to treatment clusters who were not themselves subject to the reform may nonetheless travel to access treatment.
4
Wu, Yuet, and 吳越. "Immune response of human monocyte-derived dendritic cells to co-infection of influenza virus and Streptococcus pneumoniae." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45543732.
5
McKenna, Kenneth A. (Kenneth Allen). "Assessing the Psychological Impact of Fertility Treatment." Thesis, University of North Texas, 1997. https://digital.library.unt.edu/ark:/67531/metadc277704/.
Abstract:
This controlled descriptive study was designed to investigate the psychological status of couples who are engaged in advanced fertility treatments. A battery of psychological test instruments, including the Millon Behavioral Health Inventory (MBHI), the Health Attribution Test (HAT), the Beck Depression Inventory (BDI), the Beck Anxiety Inventory (BAI), and the Marlowe-Crowne Social Desirability Scale (MCSDS), was used to measure psychological variables that have been shown in the infertility research literature to be associated with the psychological experiences of infertility patients. The scores from the four assessment instruments were compared with those of pregnant couples in childbirth education classes to differentiate the impact of stress associated with fertility treatment from the stress experienced by third trimester pregnant couples. Eighty-five subjects (42 male and 43 female) volunteered for the study and completed packets of questionnaires. The groups were designated Treatment (infertile couples) and Control (pregnant couples). The resulting data were collected and analyzed on the basis of group mean scores on the test instruments.
6
Qin, Gang, and 秦刚. "The immunological roles of human gammadelta T lymphocytes in influenzavirus infection." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46477354.
7
Yip, Ming-shum, and 葉名琛. "Severe acute respiratory syndrome coronavirus infection of human immune cells through antibody-mediated pathway." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46542139.
8
Yip, Ming-shum, and 葉名琛. "Immune responses of human respiratory epithelial cells to respiratory syncytial virus and human metapneumovirus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B3955725X.
9
SORENSON, ANN MARIE. "ETHNICITY AND FERTILITY: THE FERTILITY EXPECTATIONS AND FAMILY SIZE OF MEXICAN-AMERICAN AND ANGLO ADOLESCENTS AND ADULTS, HUSBANDS AND WIVES (BIRTHS, HISPANIC)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/188137.
Abstract:
Because pronatalist sentiments may be an important aspect of Mexican-American ethnic heritage, this research focuses on cultural as well as socioeconomic factors which may contribute to higher Mexican-American fertility. Language use and nativity are used as indirect indicators of identification with an ethnic culture. Wives' characteristics are generally considered adequate to the study of couples' fertility, but in light of earlier research by the author indicating the importance of cultural factors to the fertility expectations of Mexican-American adolescent males, characteristics of husbands as well as wives are included in this analysis. For this reason, the sample, which is drawn from the 1980 Census data for Arizona, Texas, and New Mexico, is limited to Mexican-American and Anglo women who have been married only once and live with their husbands. Two complementary methods of analysis are used. Linear regression describes the significance of husband's and wife's language use, nativity, and socioeconomic characteristics to mean family size. Parity progression ratios are used to study the contribution of these variables to the likelihood of the addition of one more child at each stage of the family building process. While wife's characteristics are sufficient to account for most of the variation observed in Anglo fertility, husband's socioeconomic characteristics significantly contribute to variation observed in the fertility of Mexican-American couples. Husbands' identification with Mexican-American culture may be somewhat more important to couples' fertility than that of their wives. This is consistent with research which suggests that children are more central to male sex role expectations as they are expressed in the context of Mexican-American culture than in that of Anglos. The measures of ethnic identity used in this study are clearly associated with socioeconomic status. The differential fertility of Anglos and Mexican Americans could be attributed to these differences. The association of Spanish language use and fertility has been linked to the lower opportunity costs represented by additional children to women who do not speak English proficiently. However, the analysis of these data, which compares structural and cultural explanations of fertility differentials, provides evidence of cultural effects as well as the effects of socioeconomic status on fertility.
10
Yuan, Tingting, and 袁婷婷. "Identification of intermediate antibodies of broadly neutralizing HIV-1 human monoclonal antibody b12 and characterization of variable loops of HIV-1 envelop glycoprotein." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196445.
11
Yim, Chi-ho Howard, and 嚴志濠. "Cytokine dysregulation by human immunodeficiency virus-1 transactivating protein." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36987700.
12
Lu, Qian, and 陸茜. "Expression and regulation of human {221}-defensins in gingival epithelia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36613708.
13
Rahim, Abdur. "Effects of women's education on fertility in rural Bangladesh : an empirical test of a causal model." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61713.
14
Lee, Jai-Wei 1970. "The effect of recombinant human interleukin-1b and interleukin-8 on bovine neutrophil migration and degranulation /." Thesis, McGill University, 1999. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21587.
Abstract:
The objective of this study was to investigate the effect of recombinant human interleukin-1beta (rHIL-1beta) and interleukin-8 (rHIL-8) on bovine neutrophil migration and degranulation. An in vitro co-culture system was used to study bovine neutrophil migration. This simulative system allowed studying neutrophil migration across endothelium (bovine aorta endothelial cells), extracellular matrix (ECM), and epithelium (MAC-T) in the correct sequences and directions. Quantification of neutrophil migration was carried out by assaying the activity of myeloperoxidase, a major enzyme of neutrophils. Degranulation of azurophilic, specific, and tertiary granules was studied by measuring releases of myeloperoxidase, lactoferrin, and gelatinase, respectively. The results showed that bovine neutrophils were able to migrate across the simulative co-culture system in response to zymosan activated serum. Recombinant HIL-8 was demonstrated to have a dose-dependent effect on bovine neutrophil migration. Furthermore, rHIL-8 had a dose-dependent effect directly on degranulation of azurophilic and specific granules, but not on tertiary granules. On the other hand, rHIL-1beta only had a significant effect on degranulation of azurophilic granules when the concentration of 100 ng/ml was used. The dose effect of rHIL-1beta on specific degranulation was much stronger. Moreover, the effect of 100 ng/ml rHIL-1beta was augmented when the rHIL-1beta containing solution was preincubated with MAC-T monolayers for four hours. This indicated that MAC-T cells might generate other degranulating factors in response to the stimulation of rHIL-1beta. These MAC-T-derived degranulating factors did not have effect on the release of tertiary granule contents.
15
Glinsmann-Gibson, Betty Jean 1961. "Molecular mechanism of autocrine regulation by TGF-alpha in T(3)M(4) human pancreatic carcinoma cells." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277113.
Abstract:
The human pancreatic cancer cell line T3M4, is known to produce transforming growth factor-alpha (TGF-alpha); as well as overexpress the receptor for this ligand, epidermal growth factor (EGF) receptor. TGF-alpha messenger RNA (mRNA) levels were assayed using northern blot, after addition of epidermal growth factor or TGF-alpha. The level of TGF-alpha mRNA was found to increase 2-fold at 2 hours and then return to near basal levels at 10 hours, after treatment with either ligand. Both ligands were also equipotent in a 2 hour dose response assay, with half maximal stimulation seen at 1 nM and maximal stimulation reached at 4 nM. Furthermore, there appeared to be a 2-fold increase in TGF-alpha transcription as determined by nuclear runoff experiments. Induction of TGF-alpha mRNA coupled with the overexpression of the EGF receptor, may result in a potent autocrine cycle; which may be found in other cancers.
16
Geary, Sean Michael. "Manipulation of the immunostimulatory capacity of a human myeloid leukaemia cell line HL-60 /." Title page, contents and abstract only, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phg292.pdf.
17
Moller, Marlo. "Human genetic susceptibility to tuberculosis : the investigation of candidate genes influencing interferon gamma levels and other candidate genes affecting immunological pathways." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/1264.
Abstract:
Thesis (PhD (Biomedical Sciences. Molecular Biology and Human Genetics))--University of Stellenbosch, 2007.The infectious disease tuberculosis (TB) is one of the leading causes of death worldwide. The idea that infectious diseases are the most important driving force in natural selection and that they sustain frequent polymorphisms in the human genome was formally suggested by Haldane in 1949. This hypothesis implicated the human genetic component in the response to infectious disease. Today the involvement of host genetics in TB has been proven unequivocally and, together with environmental factors (e.g. nutrition and crowding) and the causative bacterium, Mycobacterium tuberculosis (M.tuberculosis), may influence the outcome of disease. As is evident, TB is a complex disease and the implication for studying genetic susceptibility is that a number of genes will be involved. Interferon gamma (IFN-7) is the major macrophage-activating cytokine during infection with M.tuberculosis and its role has been well established in animal models and in humans. This cytokine is produced by activated T helper 1 (Th1) cells. These Th1 responses can best deal with intracellular pathogens such as M.tuberculosis. We selected twelve candidate genes based on the hypothesis that genes which regulate the production of IFN-7 may influence TB susceptibility. We also selected polymorphisms from 27 other candidate genes, which may affect immunological pathways involved in TB, to investigate as susceptibility factors based on the following hypotheses: 1) granulomatous diseases can share susceptibility genes; 2) gene expression studies done by DNA-array analysis experiments may reveal TB susceptibility genes; 3) genomewide linkage studies in TB can determine susceptibility loci and genes in this region are possibly susceptibility factors; and 4) functional susceptibility polymorphisms in genes involved in immune-mediated diseases other than TB may contribute to susceptibility to TB. This research tested the association of 136 genetic polymorphisms in 39 potentially important genes with TB in the South African Coloured population. Well-designed case-control association studies were used and we attempted to replicate these findings in an independent sample set using family-based case-control designs (transmission disequilibrium tests (TDTs)). In addition, haplotypes and linkage disequilibrium (LD) in the candidate genes were also investigated. During the case-control analyses we found significant associations for 6 single nucleotide polymorphisms (SNPs) in the following genes: SH2 domain protein 1A, tolllike receptor 2, class II major histocompatibility complex transactivator, interleukin 1 receptor antagonist, runt-related transcription factor 1 and tumour necrosis factor superfamily, member 1B. Discrepant results were obtained during the TDT analyses. The number of families available was small and for this reason we cannot conclude that the case-control results were spurious. We also tested the association of haplotypes with TB. Haplotypes in the interleukin 12, beta (IL12B) and toll-like receptor 4 genes were nominally associated with TB in both the case-control and TDT analyses. We observed strong LD for the genes in the South African Coloured population. In total 17 novel SNPs were identified and one novel allele was found for a microsatellite in IL12B. This research contributes to the increasing amount of information available on genes involved in TB susceptibility, which in the future may help to predict high risk individuals.
18
Varelias, Antiopi. "Studies of CD44 variant isoform expression and function on activated human peripheral blood mononuclear cells and in renal transplantation." Title page, summary and contents only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phv293.pdf.
19
Reuter, Helmuth. "The immunopathogenesis and treatment of tuberculous pericardial effusions in a population with a high prevalence of infection with the human immunodeficiency virus." Thesis, Link to the online version, 2005. http://hdl.handle.net/10019.1/1411.
20
Carael, M. "Population et santé en Afrique centrale: contribution à l'étude des déterminants sociaux de la fécondité et de l'infection au virus de l'immunodéficience humaine." Doctoral thesis, Universite Libre de Bruxelles, 1992. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212882.
21
Main, Carey Anne. "To determine the relationship between dietary intake, body composition and incidence of upper respiratory tract infections in triathletes during training and competition for the Ironman." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80006.
Abstract:
Thesis (MNutr)--Stellenbosch University, 2013.ENGLISH ABSTRACT: Background: The Ironman® triathlon is an ultra-endurance event. It has previously been shown that heavy training schedules and racing ultra-endurance events can lead to immune impairment. Evidence supporting the potential role of dietary intake and body composition on immune impairment or upper respiratory tract infections (URTIs) is currently lacking. Aim: To investigate the relationship between dietary intake, body composition and the incidence of URTI in triathletes residing in Port Elizabeth (PE), during training and competition for the Ironman® 2011 triathlon. Method: An observational longitudinal descriptive study with an analytical component was conducted. The study population included triathletes living in PE, who completed an Ironman® distance event one year prior to, and who were training for the April 2011 Ironman®. Habitual dietary intake was assessed with a quantitative food frequency questionnaire; and race dietary strategies with a three day food record. Body composition was determined with anthropometry and the incidence of URTI was assessed with the WURSS-44. A general health screen (SF-36) was also administered. Results: Habitual dietary intake during the three months pre- and post-Ironman® 2011 triathlon was adequate for all nutrients except for carbohydrate intake in female and male participants (pre-Ironman® of 4.0 (1.7) g/kg body weight (BW)/day and 5.4 (1.8) g/kg BW/day; and post-Ironman® 3.0 (1.0) g/kg BW/day and 4.7 (1.5) g/kg BW/day respectively). Carbohydrate-loading strategies were below recommendations with intakes of 6.0 (2.9) and 5.1 (2.5) g/kg BW/day for female and male participants respectively. Race day nutrition strategies were below recommendations for carbohydrate intake. Post-race dietary intake was below recommendations for carbohydrate in the female participants (0.9 (0.5) g/kg BW). Body mass index was 26.6 (3.4) kg/m2 and 26.1 kg/m2 (1.40) for female and male study participants respectively. Body fat percentage was at the upper end for endurance athletes (29.3 (9.4) % and 13.7 (5.1) % for females and males respectively). In this study 25 % of the triathletes (N=20) developed an episode of URTI during the 3 months post-Ironman®. Dietary intake parameters measured three months pre-Ironman® that had a significant influence on URTI were: potassium (p=0.04) and thiamine (p=0.02) and dietary intake parameters measured 3 months post-Ironman® that had a significant influence on URTI were: total protein (p=0.04); isoleucine (p=0.03); leucine (p=0.03); phenylalanine (p=0.03); valine (p=0.02); thiamine (p=0.01); and Beta-tocopherol (p=0.03). Dietary intake parameters measured during the race that had a significant influence on URTI were: selenium (p=0.04); folate (p=0.04) and proline (p=0.02). Body composition did not have a significant influence on URTI. Conclusion: Habitual dietary intake three months pre- and post-Ironman® as well as pre- and post Ironman race strategies were low for carbohydrate. Body composition indicated that athletes were at the upper end associated with endurance sport. There was a relationship found between an episode of URTI and dietary intake.
AFRIKAANSE OPSOMMING: Agtergrond: Die Ironman® driekamp is 'n ultra-uithouvermoë kompetisie. Daar is voorheen bewys dat swaar oefening skedules en ultra-uithouvermoë kompetisies kan lei tot ‘n immuungebrek. Daar is tans ‘n tekort aan wetenskaplike bewyse wat die potensiële rol van dieetinname en liggaamsamestelling op immuungebrek of boonste lugweginfeksies ondersoek. Doel: Die doel van die studie was om ondersoek in te stel oor die verhouding tussen dieetinname, liggaamsamestelling en die insidensie van boonste lugweg infeksies in driekamp atlete woonagtig in Port Elizabeth (PE), tydens oefening en deelname aan die Ironman® 2011 driekamp. Metodes: 'n Waargenome, longitudinale beskrywende studie is gedoen met 'n analitiese komponent. Die studiepopulasie het bestaan uit driekampatlete woonagtig in PE, wat 'n Ironman® afstand kompetisie voltooi het een jaar voor en wat oefen vir die April 2011 Ironman® kompetisie. Gewoontelike dieetinname is bepaal met 'n kwantitatiewe voedselfrekwensie vraelys, en dieet strategieë rondom die byeenkoms met 'n drie dag voedselrekord. Liggaamsamestelling is bepaal met antropometrie en die insidensie van boonste lugweg infeksies is bepaal met die WURSS-44. 'n algemene gesondheid vraelys (SF- 36) is ook ingevul. Resultate: Die gewoontelike dieetinname gedurende die drie maande voor- en na-Ironman® 2011 was voldoende vir alle voedingstowwe, behalwe vir koolhidraat-inname in die vroulike en manlike deelnemers (voor Ironman® 4.0 (1.7) g / kg liggaamsmassa (LM) / dag en 5.4 (1.8) g / kg LM / dag, en na Ironman® 3.0 (1.0) g / kg LM / dag en 4.7 (1.5) g / kg LM / dag onderskeidelik). Koolhidraatlading strategieë was ontoereikend met innames van 6.0 (2.9) en 5.1 (2.5) g / kg BW / dag vir vroulike en manlike deelnemers onderskeidelik. Die inname op die dag van die byeenkoms was onvoldoende vir koolhidraat. Die dieetinname na die byeenkoms was onvoldoende vir koolhidraat inname in die vroulike deelnemers (0.9 (0.5) g / kg LM). Die liggaamsmassa-indeks was 26.6 (3.4) kg/m2 en 26.1 (1.4) kg/m2 vir vroulike en manlike deelnemers onderskeidelik. Persentasie liggaamsvet was aan die boonste grens geassosieer met uithouvermoë oefening atlete 29.3 (9.4) % en 13.7 (5.1) % vir vrouens en mans onderskeidelik. Die insidense van boonste lugweg infeksies was 25% (N=20) gedurende die drie maande na Ironman®. Dieetinname paramters wat gemeet was drie maande voor Ironman® wat beduidende beïnvloed met boonste lugweginfeksies getoon het, was, kalium (p=0.04) en tiamien (p=0.02) en die dieetinname parameters wat drie maande na Ironman® gemeet is en betekenisvolle beïnvloed getoon het met boonste lugweginfeksies was, totale proteïen (p=0.04); isoleusien (p=0.03), leusien (p=0.03), fenielalanien (p=0.03), valien (p=0.02), tiamien (p=0.01), en B-tocopherol (p=0.03). Die dieetinname parameters wat gemeet was tydens die wedloop wat beduidende beïnvloed met boonste lugweginfeksies getoon het na Ironman® 2011 was, selenium (p=0.04), folaat (p=0.04) en prolien (p=0.02). Die antropometriese parameters gemeet het nie beïnvloed op boonste lugweginfeksies gehad nie. Gevolgtrekking: Die gewoontelike dieetinname drie maande voor- en na Ironman® sowel as voor- en na Ironman® kompetisie strategieë was onvoldoende vir koolhidrate. Liggaamsamestelling het aangedui dat atlete aan die boonste grens geassosieer met uithouvermoë oefening geval het. Daar was beduidende beïnvloed gevind tussen dieetinname en boonste lugweginfeksies.
22
Bekele, Adane Mihret. "Gene expression and cytokine pattern of pulmonary tuberculosis patients and their contacts in Ethiopia." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71942.
Abstract:
Thesis (PhD)--Stellenbosch University, 2013.ENGLISH ABSTRACT: The immune response against M. tuberculosis is multifactorial, involving a network of innate and adaptive immune responses. Characterization of the immune response, a clear understanding of the dynamics and interplay of different arms of the immune response and the identification of infection-stage specific biomarkers are critical to allow the development of better tools for combating tuberculosis. In an attempt to identify such biomarkers, we studied pulmonary tuberculosis patients and their contacts in Addis Ababa, Ethiopia as part of EDCTP and BMGF funded tuberculosis projects by using multiplex techniques. We analysed 45 genes using the Multiplex Ligation Dependent Probe Amplification (MLPA) technique and the expression of IL-4δ2, BLR1, MARCO, CCL-19, IL7R, Bcl2, FcyR1A, MMP9, and LTF genes discriminate TB cases from their healthy contacts. FoxP3, TGFß1 and CCL-19 discriminate latently infected from uninfected contacts. Single genes predict with an area under the Receiver Operating Characteristic (ROC) curve of 0.68 to 0.85 while a combination of genes identified up to 95% of the different groups. Similarly, the multiplex analysis of cytokines and chemokines also showed that single or combinations of plasma cytokines and chemokines discriminate between different clinical groups accurately. The median plasma level of EGF, fractalkine, IFN-y, IL-4, MCP-3 and IP-10 is significantly different (p<0.05) in active tuberculosis and non active tuberculosis infection and the median plasma levels of IFN-y, IL-4, MCP-3, MIP-1ß and IP-10 were significantly different (p<0.05) before and after treatment. We also found a significant difference (p<0.05) in plasma levels of cytokines of patients infected with the different lineages and different families of the modern lineage. The plasma level of IL-4 was significantly higher in patients infected with lineage 3 (p<0.05) as compared to lineage 4 and the CAS familyinfected patients had a higher plasma level of IL-4 (P<0.05) as compared to patients infected with H and T families but there was no difference between H and T families. We identified genes and cytokines which had been reported from other studies in different settings and we believe that these molecules are very promising biomarkers for classifying active tuberculosis, latent infection, absence of infection and treated infection. These markers may be suitable for the development of clinically useful tools but require further validation and qualification in different populations and in larger studies.
AFRIKAANSE OPSOMMING: Die immuunrespons teen M. tuberculosis is multifaktoriaal en betrek ‘n netwerk van niespesifieke and spesifieke immuunresponse. Karakterisering van die immuunrespons, ‘n duidelike insig in die dinamika en tussenspel deur die verskillende arms van die immuunrespons en die identifikasie van spesifieke biomerkers is krities belangrik om die ontwikkeling van nuwe hulpmiddels teen tuberkulose te bevorder. In ‘n poging om sulke biomerkers te identifiseer het ons pulmonale tuberkulose pasiënte en hulle kontakte in Addis Ababa, Etiopië, as deel van die EDCTP en BMGF befondste tuberkulose projekte bestudeer met multipleks tegnieke. Ons het 45 gene analiseer met ‘Multiplex Ligation Dependent Probe Amplification (MLPA)’ en gevind dat die geenuitdrukking van IL-4•2, BLR1, MARCO, CCL-19, IL7R, Bcl2, Fc•R1A, MMP9, en LTF TB pasiënte van hulle kontakte onderskei. FoxP3, TGF•1 en CCL-19 onderskei tussen latent infekteerde en ongeïnfekteerde kontakte. Enkele gene voorspel met ‘n area onder die ‘Receiver Operating Characteristic (ROC)’ kurwe van 0.68 tot 0.85 terwyl die kombinasie van gene 95% van die verskillende groepe identifiseer. Soortgelyk het multipleks analise van sitokiene en chemokiene verskillende kliniese groepe akkuraat van mekaar onderskei. Die mediane plasmavlakke van EGF, fractalkine, IFN-•, IL-4, MCP-3 en IP-10 is beduidend verskillend (p<0.05) in aktiewe tuberkulose en nie-aktiewe tuberkulose infeksie en die mediane plasmavlak van IFN-•, IL-4, MCP-3, MIP-1• en IP-10 was beduidend verskillend voor en na behandeling. Ons het ook beduidende verskille (p<0.05) in plasmavlakke van sitokiene in pasiënte gevind wat infekteer is met verskillende stamme and verskillende families van die moderne stamme. Die plasmavlak van IL-4 was beduidend hoër in pasiënte wat infekteer is met stam 3 (p<0.05) teenoor stam 4 en die CAS familie-infekteerde pasiënte het ‘n hoër plasmavlak van IL-4 (p<0.05) teenoor pasiënte met H en T familie infeksie hoewel daar geen versikke was tussen die H en T families nie. Ons het gene en sitokiene identifiseer wat deur ander werkers onder verskillende omstandighede ook beskryf is en ons glo dat hierdie molekules baie belowende biomerkers is om aktiewe tuberkulose, latent tuberkulose, die afwesigheid van infeksie en behandelde infeksie van mekaar te onderskei. Hierdie merkers mag toepaslik wees vir die ontwikkeling van bruikbare kliniese hulpmiddele maar benodig verdere validasie en kwalifikasie in verskillende populasiegroepe en in groter studies.
Bill and Melinda Gates Foundation (BMGF)
European and Developing Countries Clinical Trials Partnership (EDCTP)
African European Tuberculosis Consortium (AE TBC).
23
Abraham, Yisa Thomas. "Stigmatization and VVF-HIV/AIDS among young adults females : a critical pastoral assessment of the role of the ECWA (Evangelical Church West Africa)." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80108.
Abstract:
Thesis (PhD)--Stellenbosch University, 2013.ENGLISH ABSTRACT: This study focuses on the problem of VVF-HIV/AIDS, stigmatization, the threat to the human dignity of women and the role of the church, with specific reference to the role of Evangelical Church Winning All (ECWA). In order to show this, models of practical theology methodology were used as theoretical and methodological basis for the study. Practical theology is as a study area deals with the praxis of God, i.e. salvific and eschatological involvement and engagement with the trajectories of human lives and the suffering of human beings. Within the context of theological reflection, it involves man’s attempt to express and portray the presence and will of God in such a way that meaning in life and comfort is contextually disclosed and discovered (Louw, 2008:71). Having established the latter, the focus falls firstly on the description of the conditions addressed in the study about VVF-HIV/AIDS and its prevalence in Northern Nigeria. A detailed contextual study also shows that a variety of factors impact negatively on the status and well-being of women in the area. Traditional, cultural, economic, political and religious factors are either uniquely applicable to or aggravate the status and well-being of the subjects of the research, namely women suffering from VVF-HIV/AIDS in Northern Nigeria. It specifically involves the social and political context in which they live. It also shows that the existence and extent of these factors increase the vulnerability of women to contracting the HIV as well as VVF. The extent to which these factors, in combination with the latter conditions specifically promote the stigmatization of these women and the forms such stigmatization takes are also explored. Moving on to the issue of human dignity: a historical overview is given of the concept and it is defined for the purposes of the study. The extent to which the human dignity is affected in the study area is then investigated in light of their context, with particular reference to the women suffering from VVF-HIV/AIDS. It is concluded that the stigmatization to which the VVF-HIV/AIDS sufferers in Northern Nigeria are subjected, indeed constitutes a serious threat to their human dignity. In answering the question of whether the church (ECWA) has a responsibility towards these women and to address the issue of their stigmatization, two pastoral theological perspectives were used, that of the nature of the church and that of the concept of human dignity from a theological perspective. According to this perspective human beings have been created in the image of God. Having established that, on theological grounds, such a responsibility exists, a possible pastoral theological model for addressing the issue of the stigmatization of women suffering from VVF-HIV/AIDS was proposed. The church’s response to the challenge of VVF-HIV/AIDS is to come from its deepest theological convictions about the nature of creation, the unshakeable fidelity of God’s love, the nature of creation, the nature of the body of Christ and the reality of Christian hope. The creation narrative, which affirms that humanity is created in the image of God, links human beings to the love of God, which is modelled in the incarnation of Jesus. Moving on to the data analysis, the extent of the challenges of VVF-HIV/AIDS sufferers and the level of knowledge of the pastors of the subject of the stigmatization of young adult females sufferers of VVF-HIV/AIDS and their treatment of the issue were evident. Finally, recommendations were drawn up in order to provide basic understanding and awareness to ECWA on how to objectively address the problem of VVF-HIV/AIDS in Northern Nigeria.
AFRIKAANSE OPSOMMING: Hierdie studie fokus op die probleem van VVF-HIV/AIDS, stigmatisering, die bedreiging van die menslike waardigheid van vroue en die rol van die kerk (ECWA). Om dit aan te toon, word die model van die praktiese teologie metodologie gebruik as 'n teoretiese en metodologiese basis vir die studie. Praktiese teologie handel oor die praxis van God, d.w.s. die verlossingsboodskap en eskatologiese betrokkenheid by en verbintenis met die trajekte van die menslike lewe en die lyding van die mens. Binne die konteks van teologiese refleksie, d.w.s. die menslike poging om aan 'n beeld van die teenwoordigheid en wil van God op so 'n manier uitdrukking te gee, word die betekenis daarvan in die lewe en troos kontekstueel geopenbaar en ontdek (Louw, 2008:71). Na laasgenoemde val die fokus eers op die beskrywing van die voorwaardes in die studie oor VVF-HIV/AIDS en die voorkoms daarvan in die noorde van Nigerië. ’n Gedetailleerde kontekstuele studie toon ook dat 'n verskeidenheid negatiewe faktore ‘n impak op die status en die welsyn van vroue in die area het. Tradisionele, kulturele, ekonomiese, politieke en godsdienstige faktore waarvan 'n paar óf uniek van toepassing is óf ‘n verswarende effek het op die navorsingskonteks van vroue wat in die noorde van Nigerië aan VVF-HIV/AIDS ly en spesifiek op die sosiale, politieke konteks waarin hulle leef. Daar word ook aangetoon dat die bestaan en omvang van hierdie faktore die vatbaarheid van vroue vir die kontraktering van die MIV-virus sowel as VVF, verhoog. Daar word ook gekyk na die wyse waarop hierdie faktore in kombinasie met bogenoemde voorwaardes spesifiek die bevordering van die stigmatisering van hierdie vroue teweegbring en na die vorme wat hierdie stigmatisering aanneem. Die kwessie van menslike waardigheid word ondersoek deur 'n historiese oorsig van die konsep te gee. Dit word vir die doeleindes van die studie gedefinieer. Die mate waarin menslike waardigheid in die studiearea ‘n rol speel, met spesifieke klem op die konteks van vroue wat ly aan VVF-HIV/AIDS, word ook nagegaan. Daar word tot die gevolgtrekking gekom dat die menswaardigheid van die VVF-HIV/AIDS lyers in die noorde van Nigerië tot 'n groot mate in die lig van die stigmatisering hulle aan onderwerp word, aangetas word. Ter beantwoording van die vraag of die kerk (ECWA) 'n verantwoordelikheid teenoor hierdie vroue het om hul stigmatisering aan te spreek, word twee pastorale teologiese perspektiewe gebruik: dié van die aard van die kerk en van die konsep van menswaardigheid vanuit 'n teologiese perspektief waarvolgens die mens na die beeld van God geskep is. Nadat vasgestel is dat, op teologiese gronde, so 'n verantwoordelikheid wel bestaan, word 'n moontlike pastorale teologiese model vir die aanspreek van die kwessie van die stigmatisering van vroue wat ly aan VVF-HIV/AIDS voorgestel. Die kerk se reaksie op die uitdaging van VVF-HIV/AIDS spruit uit sy diepste teologiese oortuigings oor die onwrikbare getrouheid van God se liefde, die aard van die skepping, die aard van die liggaam van Christus en die werklikheid van die Christelike hoop. Die skeppingsverhaal, wat bevestig dat die mensdom in die beeld van God geskep is, verbind die mens aan die liefde van God, wat in die inkarnasie van Jesus gemodelleer word. Daar word dan beweeg na die data-analise, die omvang van die uitdagings van VVF-HIV/AIDS lyers en die vlak van kennis van die pastore oor die onderwerp van die stigmatisering van die jong volwasse vroulike lyers aan VVF-HIV/AIDS en hulle behandeling van die probleem. Ten slotte word aanbevelings gemaak ten einde basiese begrip/bewustheid te verskaf oor hoe die ECWA die probleem van VVF-HIV/AIDS in die noorde van Nigerië objektief kan aanspreek.
24
"An economic analysis of fertility in Hong Kong." Chinese University of Hong Kong, 1985. http://library.cuhk.edu.hk/record=b5885561.
25
"An economic analysis of birth behavior in Hong Kong." 2000. http://library.cuhk.edu.hk/record=b5890400.
Abstract:
Lai Tak Chi.Thesis (M.Phil.)--Chinese University of Hong Kong, 2000.
Includes bibliographical references (leaves 69-72).
Abstracts in English and Chinese.
Acknowledgments --- p.ii
English Abstract --- p.iii
Chinese Abstract --- p.iv
Table of Contents --- p.v
List of Tables --- p.vii
List of Figures --- p.viii
List of Appendices --- p.ix
Chapter Chapter 1. --- Introduction --- p.1
Chapter Chapter 2. --- Literature Reviews --- p.8
Chapter 2.1 --- Theoretical Approach of Household Fertility Decision --- p.8
Chapter 2.2 --- Modeling of Household Fertility Decision --- p.17
Chapter 2.2a. --- Linear Regression Model --- p.17
Chapter 2.2b. --- Count Data Models --- p.18
Chapter 2.2c. --- Goodness of Fit --- p.23
Chapter 2.3 --- Summary and Limitations --- p.25
Chapter Chapter 3. --- Data Sources and Limitations --- p.26
Chapter 3.1 --- Data Sources of the Cross-Section Analysis --- p.26
Chapter 3.2 --- Data Sources of the Time-Series Analysis --- p.26
Chapter 3.3 --- Data Limitations of the Cross-Section Analysis --- p.27
Chapter 3.4 --- Data Limitations of the Time-Series Analysis --- p.27
Chapter Chapter 4. --- Decision of Birth --- p.29
Chapter 4.1 --- Variable Definitions and Explanations --- p.29
Chapter 4.2 --- Statistical Framework --- p.33
Chapter 4.3 --- Results and Explanations for the Regression of the Decision of Birth --- p.33
Chapter 4.4 --- Summary --- p.36
Chapter Chapter 5. --- Fertility Behavior --- p.38
Chapter 5.1 --- Variable Definitions and Explanations --- p.38
Chapter 5.2 --- Statistical Framework --- p.40
Chapter 5.3 --- Empirical Results --- p.42
Chapter 5.4 --- Summary --- p.54
Chapter Chapter 6. --- Time Series Analysis --- p.56
Chapter Chapter 7. --- Conclusions --- p.63
Appendices --- p.65
Bibliography --- p.69
26
Lyons, A. B. "Human myeloid differentiation antigens / Alan Bruce Lyons." 1987. http://hdl.handle.net/2440/21575.
Abstract:
Bibliography: leaves 154-185iv, 185 leaves, [15] leaves of plates : ill. (some col.) ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Thesis (Ph.D.)--University of Adelaide, Dept. of Science, 1987
27
Hutaserani, Suganya. "Rural labor markets and fertility in Thailand : an extension of the new household economics to integrate institutional and supply-side aspects." Thesis, 1985. http://hdl.handle.net/10125/9611.
28
"Economic factors and institutional change in determining fertility in China: an empirical study." Chinese University of Hong Kong, 1991. http://library.cuhk.edu.hk/record=b5887000.
Abstract:
by Ho Sau Lan.Thesis (M.Phil.)--Chinese University of Hong Kong, 1991.
Includes bibliographical references (leaves 82-85).
ABSTRACT
ACKNOWLEDGEMENT
CONTENTS
LIST OF TABLES
CHAPTERS
Chapter 1. --- DEMOGRAPHIC PATTERN AND POPULATION POLICIES
Chapter 1.1 --- Introduction
Chapter 1.2 --- Current literature on China's demography
Chapter 1.3 --- Recent demographic trend in China
Chapter 1.4 --- Policies for controlling birth
Chapter 2. --- EMPIRICAL ANALYSIS OF THE DEMOGRAPHIC TRANSITION
Chapter 2.1 --- Explanations of the demographic transition
Chapter 2.2 --- Granger-causality
Chapter 2.3 --- Test specification
Chapter 2.4 --- Data specification
Chapter 2.5 --- Test procedure
Chapter 2.6 --- Empirical results
Chapter 2.7 --- Summary
Chapter 2.8 --- Problem of the tests
Chapter 3. --- FERTILITY CHANGE IN THE REFORM PERIOD1979-1987
Chapter 3.1 --- The economic reform
Chapter 3.2 --- Effects of the economic reform and other economic factors on fertility
Chapter 3.3 --- Data specification
Chapter 3.4 --- Statistical specification
Chapter 3.5 --- Empirical Results
Chapter 3.6 --- Summary
Chapter 4. --- CONCLUDTNG REMARKS
Chapter 5. --- APPENDIX A： GLOSSARY OF SOME DEMOGRAPHIC TERMS
Chapter 6. --- APPENDIX B： SOURCES OF DATA
Chapter 7. --- BIBLIOGRAPHY
29
Kramer, Kenton Jay. "A seroepidemiological study of human antibodies to the major merozoite surface coat precursor protein of Plasmodium falciparum (GP195) from a hyperendemic area of the Philippines." Thesis, 1990. http://hdl.handle.net/10125/9444.
30
Shale, Mashale. "Fertility transition in Lesotho : the recent trends, socioeconomic factors and proximate determinants." Thesis, 2011. http://hdl.handle.net/10413/8636.
Abstract:
There is a general perception that fertility has been declining over a decade in Lesotho, and this has
sparked the debate that fertility transition is drawing closer in Lesotho. The growing concern was
stimulated by limited studies showing the effect of socio-economic factors on fertility in Lesotho and
variations in proximate determinants. The paper examines recent fertility trends in Lesotho using various
demographic techniques of fertility estimation and determines whether the onset of fertility transition has
begun in Lesotho. The secondary aim is to assess and control errors in the Lesotho Demographic and
Health Survey of 2004, thus providing robust and reliable estimates.
The analysis utilizes the secondary data from 2004 Lesotho Demographic and Health Survey (LDHS). The
data set comprised of a sample of 7095 women who participated in the survey. The use of 1996 Lesotho
Population Census and 2002 Lesotho Reproductive and Health Survey were made to facilitate comparison
with 2004 LDHS, and to provide differentials and measure changes over time in fertility. The P/F ratio
method developed by Brass and the modified version, Relational Gompertz Model are employed and used
to assess the quality of data as well as determining fertility levels and trends. The findings reveal that the overall fertility among women in Lesotho during 2004 LDHS is 4.02.
Application of different methods depicts that fertility remains high in Lesotho, although considered
moderate according to sub-Saharan standards. Despite the fact that TFR is high, overall fertility decline is
evident. The estimates of fertility range between 3.5 and 5.6 depending on the technique in use. The reason
for the high observed fertility is that women in the rural areas still cherish quite a substantial family size.
Nevertheless, changing acceptance and perception of using contraception, delayed marriage, high levels of
education and economic development among women in Lesotho contributes considerably to fertility
declines in Lesotho. As a result, disparities that continue to propel fertility levels within population groups
incite reassessment of existing research and policy so as to enhance development strategies as well as
action programmes.Thesis (M.A.)-University of KwaZulu-Natal, Howard College, 2011.
31
Byrd, Daniel James. "Vaccinia Virus Binding and Infection of Primary Human Leukocytes." Thesis, 2014. http://hdl.handle.net/1805/5279.
Abstract:
Indiana University-Purdue University Indianapolis (IUPUI)Vaccinia virus (VV) is the prototypical member of the orthopoxvirus genus of the Poxviridae family, and is currently being evaluated as a vector for vaccine development and cancer cell-targeting therapy. Despite the importance of studying poxvirus effects on the human immune system, reports of the direct interactions between poxviruses and primary human leukocytes (PHLs) are limited. We studied the specific molecular events that determine the VV tropism for major PHL subsets including monocytes, B cells, neutrophils, NK cells, and T cells. We found that VV exhibited an extremely strong bias towards binding and infecting monocytes among PHLs. VV binding strongly co-localized with lipid rafts on the surface of these cell types, even when lipid rafts were relocated to the cell uropods upon cell polarization. In humans, monocytic and professional antigen-presenting cells (APCs) have so far only been reported to exhibit abortive infections with VV. We found that monocyte-derived macrophages (MDMs), including granulocyte macrophage colony-stimulating factor (GM-CSF)-polarized M1 and macrophage colony-stimulating factor (M-CSF)-polarized M2, were permissive to VV replication. The majority of virions produced in MDMs were extracellular enveloped virions (EEV). Visualization of infected MDMs revealed the formation of VV factories, actin tails, virion-associated branching structures and cell linkages, indicating that infected MDMs are able to initiate de novo synthesis of viral DNA and promote virus release. Classical activation of MDMs by LPS plus IFN-γ stimulation caused no effect on VV replication, whereas alternative activation of MDMs by IL-10 or LPS plus IL-1β treatment significantly decreased VV production. The IL-10-mediated suppression of VV replication was largely due to STAT3 activation, as a STAT3 inhibitor restored virus production to levels observed without IL-10 stimulation. In conclusion, our data indicate that PHL subsets express and share VV protein receptors enriched in lipid rafts. We also demonstrate that primary human macrophages are permissive to VV replication. After infection, MDMs produced EEV for long-range dissemination and also form structures associated with virions which may contribute to cell-cell spread.
32
Leibelt, Dustin A. "Chronic exposure of rodents to indole-3-carbinol and 3,3'-diindolylmethane : implications for drug metabolism, chemoprevention and human health." Thesis, 2003. http://hdl.handle.net/1957/31050.
Abstract:
Indole-3-carbinol (I3C) is a naturally occurring plant alkaloid, found in
significant concentrations in cruciferous vegetables such as broccoli and Brussels
sprouts. I3C is an unstable compound that undergoes rapid oligomerization in an
acidic environment to form higher order condensation products (I3C-ACPs), such
as 3-3'-diindolylmethane (DIM). Both I3C and DIM are marketed as dietary
supplements and are under investigation as potential chemopreventive agents,
despite limited data on the effects of chronic exposure. Previous studies have
demonstrated that the chemopreventive potential of I3C and DIM in animal studies
is dependent on species, strain, tissue and timing of treatment relative to carcinogen
exposure, and long-term post-initiation exposure can even promote tumors. The
majority of biological effects from I3C are the result of the abilities DIM and other
I3C-ACPs to bind to the aryl hydrocarbon receptor and the subsequent induction of
phase I and phase II enzymes. Phase I and phase II enzyme induction in many
cases leads to protection from carcinogens by increasing the rate of metabolism and
excretion but in some cases enhances carcinogenicity by increasing the rate of
bioactivation. It has been demonstrated that modulation of enzyme levels can also
result in altered metabolism of compounds that could affect efficacy and toxicity of
pharmaceuticals and xenobiotics. The current work utilizes chronic dietary I3C and
DIM exposures in rodent models to further elucidate the effect these compounds
might have on health, drug metabolism and carcinogenesis. The reduced weight of
Fischer 344 rats treated with 2500 ppm I3C for 1 year may be indicative of adverse
effects but toxicity was not confirmed by blood chemistry or histopathological
examination. Furthermore, no toxicity was observed after a comparable treatment
of Sprague-Dawley rats. As observed after acute and sub-chronic exposures to I3C
and DIM, we documented significant induction of cytochrome P450 enzymes and a
related modification to drug metabolism in liver slice incubations. Evidence is also
provided that may suggest that tumor modulation in mice may occur through an
estrogenic mechanism. Further studies should be completed to determine the
potential for similar responses in humans.Graduation date: 2004
33
"Cyclic changes in uterine CFTR expression, bicarbonate secretion and fluid volume: implications in fertility and infertility." Thesis, 2008. http://library.cuhk.edu.hk/record=b6074599.
Abstract:
Further studies were conducted to define an indispensable role of uterine bicarbonate secretion at pre-implantation for the success of blastocyst implantation. The in vitro implantation experiments showed that only when cultured in bicarbonate-containing medium, the blastocysts exhibited normal rate of attachment and outgrowth level. The forskolin-induced endometrial bicarbonate secretion measured by the Isc on pregnant day 4 was almost abolished by CA inhibitor acetazolamide. The efflux of intracellular bicarbonate, measured by intracellular pH-sensitive dye, was blocked by CFTR inhibitor, NPPB, and SLC26a6 inhibitor, DIDS, indicating their involvement in mediating uterine bicarbonate secretion.In conclusion, the present findings have demonstrated an important role of CFTR in formation of optimal uterine fluid, in terms of both volume and composition, which is crucial for various reproductive events occurring in the uterus. Deviation from the normal uterine fluid composition and volume due to defects in CFTR function or abnormal regulation under pathological conditions, such as CF and genital bacteria infection, probably leads to infertility. The information obtained may provide insight into regulatory mechanism underlying fertility and infertility, as well as the rationale for development of treatment methods for female infertility and new strategies for female contraception. (Abstract shortened by UMI.)
The last part of the study was to demonstrate possible cause of infertility by disturbance of uterine fluid dynamic due to abnormal expression of CFTR using a model of uterine Chlamydia (C.) trachomatis infection, the most common infection-related sterility with the underlying cause unexplained. Uterine C. trachomatis infection induced up-regulated expression of CFTR with enhanced electrolyte and fluid transport as demonstrated by the increase in the cAMP-dependent Isc and uterine wet weight with obvious fluid accumulation in the lumen at diestrus stage, during which the endometrium normally undergoes a series of changes preparing for blastocyst implantation with minimum CFTR expression and uterine fluid volume. The abnormal uterine fluid accumulation upon uterine C. trachomatis infection significantly reduced implantation rate in uterine C. trachomatis infection mouse model.
The present study was aimed to elucidate the cellular and molecular mechanisms underlying the CFTR-related reproductive events in physiological and pathological conditions by using a variety of techniques, including RT-PCR, Western blot, intracellular and extracellular pH measurements, and the short-circuit current (Isc) measurement, in conjunction with mouse primary culture of endometrial cells and blastocyst, as well as several animal models including CF mouse, mouse uterine infectious model and overyectomized (OVX) mouse, etc.
We first examined dynamic changes in uterine bicarbonate secretion, as indicated by bicarbonate-dependent forskolin-induced Isc and epithelial surface pH measurement, and the expression profile of candidate genes and proteins known to be involved in bicarbonate secretion throughout the estrous cycle in mouse uterus. The results showed that the maximum mRNA and protein levels of CFTR, SLC26a6, carbonic anhydrase (CA)2 and CA12 were observed at proestrus stage and/or estrus stages. Luminal surface pH measured by 5-N-hexadecanoyl-aminofluorescein (HAF) showed that the basal endometrial epithelial surface pH at estrus stage was significantly higher than that in diestrus, which could be reduced significantly by CFTR inhibitor DPC, SLC26a6 inhibitor 4',4'-Diisothiocyanostilbene-2',2' Disulfonic Acid (DIDS) and CA suppressor acetazolamide. In the ovariectimized (OVX) mice and primary culture of endometrial cells, estrogen could induce up-regulation of CFTR, SLC26a6, CA2 and CA12 expression with corresponding increase in the bicarbonate-dependent Isc, suggesting a novel role of estrogen in regulating uterine bicarbonate secretion.
He, Qiong.
Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3247.
Thesis (Ph.D.)--Chinese University of Hong Kong, 2008.
Includes bibliographical references (leaves 163-176).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstracts in English and Chinese.
School code: 1307.
34
Manzini, Nontsikelelo. "The impact of women's education on fertility in KwaZulu-Natal : 1993- 1998." Thesis, 2000. http://hdl.handle.net/10413/2639.
Abstract:
This article examines the relationship between women's education and fertility in KwaZulu Natal based on data from the 1993 Project for Statistics on Living Standards and Development survey (PSLSD) and the 1998 KwaZulu Natal Income Dynamics Study (KIDS). This study shows that fertility has declined between 1993 and 1998. Additionally, fertility declines as the level of education increases. However, women with lower levels of education have higher fertility than those with no schooling and women with tertiary education have higher fertility than those with secondary education. Moreover, education has a stronger effect on fertility in 1998.Thesis (M.Sc.U.R.P.)-University of Natal, 2000.
35
Yeatman, Sara Elizabeth 1979. "Childbearing in an AIDS epidemic." 2008. http://hdl.handle.net/2152/17970.
Abstract:
The consequences of the African AIDS epidemic are growing--not just in size--but in complexity. These consequences are no longer just biological; increasingly, they are also social, cultural, economic, and psychological. In this dissertation, I consider one overlooked consequence of the epidemic by asking how HIV infection affects the desire to have children in a context where reproduction is so highly valued. Taking advantage of a unique situation in rural Malawi, where no one knew their HIV status prior to testing being introduced as part of an ongoing longitudinal survey, I use a quasiexperimental design and in‐depth interviews to examine the evidence for an intentional relationship between HIV/AIDS and fertility. Rural Malawians adjust their childbearing desires in response to information about their HIV status. The relationship--both in magnitude and in motivation--is highly gendered. HIV positive women fear that a pregnancy will worsen their disease. Despite this widely shared belief, there remains a lot of ambivalence: women who are positive, or who fear they are positive, want to live normal lives. For some, that means avoiding childbearing as a strategy to delay the symptoms of HIV. For others, it means having children as they would have had despite what they think it might mean for their health. Male fertility preferences are more volatile to information about HIV status. Men see childbearing as futile if they are HIV positive because they anticipate their own death and the death of their future offspring. However, men may be less likely to translate their preferences into action because--after learning they are infected--they are less motivated to stop having children than they are unmotivated to have children. This dissertation shows that rural Malawians adapt their childbearing preferences to information about their HIV status. There are strategies in these adaptations, as well as hope for a future where the conditions of childbearing in an AIDS epidemic might have changed. I conclude by discussing what the findings mean for fertility, fertility theory, and policy.text
36
"Immunoregulatory role of human islet amyloid polypeptide through FoxP3+CD4+CD25+ T regulatory cells." Thesis, 2010. http://library.cuhk.edu.hk/record=b6075044.
Abstract:
Background. Islet amyloid polypeptide (IAPP, also known as amylin) is a 37-amino acid peptide principally co-secreted with insulin from the beta-cells of the pancreatic islets. Some of the physiological actions of human amylin (hIAPP) include glucose regulation, suppression of appetite and stimulation of renal sodium and water reabsorption. Amylin deficiency and diminished post-prandial amylin response have been reported in advanced stages of type 1 and type 2 diabetes. In autopsy specimens of type 2 diabetes, amyloid is found in 40--90% of cases. During the characterization of islet morphology of aged hIAPP transgenic mice, I observed pathological features suggestive of immune dysregulation. Review of literature also suggested possible immuno-modulating functions of human amylin in in vitro experiments. Since autoimmunity and innate immunity are implicated in aging and diabetes, I explored the immunological role of amylin with particular focus on CD4+CD25+ T regulatory cells and toll-like receptors (TLR) which are known mediators of autoimmunity and innate immunity respectively.Conclusions. Human amylin may play an important role in modulating immunity mainly through stimulating CD4+CD25+ Treg cells, decreasing PLN and altering expression of TLR-4 and cytokines. If these findings are confirmed in in vivo model, human amylin has the potential to become a novel and promising therapy to prevent and reverse autoimmune disease such as autoimmune type 1 diabetes.
Hypothesis. Human amylin may have immunomodulating effects which may have implications on pathogenesis of autoimmune type 1 diabetes.
Materials and methods. Male hemizygous hIAPP transgenic mice (n=32) and their nontransgenic littermates (n=20) were fed with normal chow and studied longitudinally up to 18 months of age with measurement of plasma insulin, glucose and amylin at regular intervals. Detailed oral glucose tolerance test, intra-peritoneal insulin tolerance test, insulin and amylin protein expression were examined at 3, 7, 12 and 18 months of age. Histological changes of pancreas and spleen including changes in CD4+CD25+ T regulatory cells and cytokines were examined at 12 and 18 months.
Objectives. (1) I systemically characterized the morphological, functional and immune regulatory role of human amylin in aged hIAPP transgenic mice which include metabolic profiles, plasma levels of amylin and insulin as well as morphological changes of pancreatic lymph nodes (PLN). (2) I then examined splenic expression of TLR-4 associated changes in cytokines (TNF-alpha, TGF-beta, and IL-6). (3) I also examined the expression level of receptor activity modifying proteins (RAMPs) in pancreas and spleen. (4) I finished by investigating the role of human amylin on stimulating CD4+CD25+ T regulatory (Treg) cells in hIAPP transgenic mice and peripheral blood monocytes (PBMC) from healthy subjects.
Results. (1) With aging, the hIAPP transgenic mice demonstrated increased plasma amylin, decreased plasma insulin, reduced insulin to amylin ratio and improved insulin sensitivity (p<0.05). (2) The aged hIAPP transgenic mice showed changes in immune function as indicated by: (a) Reduced number and size of PLN (p<0.05). (b) Decreased expression level of TLR-4 in splenocytes (p<0.05). (c) Increased expression of transforming growth factor-beta (TGF-beta) and tumor necrosis factor-alpha (TNF-alpha) protein but decreased level of IL-6 in splenocytes (p<0.05). (3) The changes in the levels of immune cytokines such as IL-1, IL-2, IL-4, IL-10, IL-17, interferon-gamma and GM-CSF were similar between hIAPP transgenic and nontransgenic mice (p>0.05). (4) The levels of RAMP1, RAMP2, and RAMP3 were higher in the spleen of hIAPP transgenic mice than nontransgenic mice (p<0.05). (5) The hIAPP transgenic mice showed higher percentage of CD4+CD25+ Treg cells compared with nontransgenic littermates. Treatment with human amylin, but not rat amylin, increased the percentage of FoxP3+CD4+CD25+ Treg cells in both splenic T lymphocytes of hIAPP transgenic mice and PBMCs of healthy subjects ex vivo (p<0.05).
He, Lan
Adviser: Juliana C.N. Chan.
Source: Dissertation Abstracts International, Volume: 73-01, Section: B, page: .
Thesis (Ph.D.)--Chinese University of Hong Kong, 2010.
Includes bibliographical references (leaves 176-199).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstract also in Chinese.
37
Dememew, Zewdu Gashu. "Fertility desire, intention and associated factors among people living with HIV seeking chronic HIV care at health facilities of Hawassa City, southern Ethiopia." Diss., 2014. http://hdl.handle.net/10500/20704.
Abstract:
Text in EnglishINTRODUCTION: Late in HIV epidemic while HIV program is maturing studies in rich and resource limited setting have shown controversial results with regard to whether childbearing desire and intention are changed after the expansion of ART and PMTCT services. There are few studies in Ethiopia which tried to find out fertility preferences after the decentralized ART and PMTCT services. PURPOSE: The objective of the study is to determine the prevalence of fertility desire, intention and associated factors among HIV positive males and females at health facilities in Hawassa city with chronic HIV care. METHOD: The study used quantitative, observational, analytic and cross-sectional study design. It was structured on Trait-Desire-Intention-Behaviour theoretical frame work. A gender based stratification followed by random sampling method was applied. An interviewer-administered structured data collection approach using the pre-tested questionnaire was applied in the study. The Microsoft Office Excel 2007 and Epi-Info version 3.5.3 were utilized for data analysis. In addition to descriptive statistics, both bivariate and multivariable logistic regressions were used to analyse the data. RESULT: With a respondent rate of 93%, a total of 460 PLHIV participated in the study with equal number of males and females. The majority of the participants were from urban (85%), in relationship (70.9%), and on ART (80%). The reported fertility desire, 43.9% (45.2% in males; 42.6% in females), and fertility intention, 44.9% (46.4% in males; 43.4% in females), were high. The median number of intended children was 2. About 54% of PLHIV were using at least one of the contraceptives with 32.4% of unmet need of family planning. Participants with overall experinece of 2 births or less (AOR: 2.4 95% CI 1.32-4.32; p-value=0.0042), without birth experience after HIV diagnosis (AOR:0.52 95% CI 0.28-0.98; p-value=0.0424) and whose partner also desired for childbearing (AOR: 19.73 95%CI 10.81-35.99; p-value=0.0000) were more likely to intend for a/another child.They wished and planned to get birth because; they did not have a/children before or fear of childless stigma (25.3%), ART could help to have negative child (21.8%), importance of parenthood (17.8%) and the desire of once partner (16.8%). The study participants had consulted health care workers (34.2%), approached their partner or their partner had already approached them (27.6%), tried to get a partner or married (17.6%) and stop using family planning (6%) to get pregnant. CONCLUSION: This study highlights high fertility desire and intention in the background of high unmet need for family planning among PLHIV. A development of comprehensive male partner-involved couple counseling protocol, improving the communication HCWs have with PLHIV to emphasize safer conception methods and strengthening all the components of PMCT integrating with other SRH services at chronic HIV clinic are critical.
Health Studies
M.A. (Public Health)
38
Monyai, Florina Semakaleng. "Establishment of interaction partners of Plasmodium falciparum heat shock protein 70-x(PfHsp 70-x)." Diss., 2018. http://hdl.handle.net/11602/1113.
Abstract:
MSc (Biochemistry)Department of Biochemistry
Plasmodium falciparum is a unicellular protozoan parasite that causes malaria in humans. The parasite is passed to humans through mosquito bites and migrates to the liver before it infects host erythrocytes. It is at the erythrocytic stage of development that the parasite causes malaria pathology. Malaria is characterized by the modification of host erythrocytes making them cytoadherent. This is as a result of formation of protein complexes (knobs) on the surface of the erythrocyte. The knobs that develop on the surface of the erythrocyte are constituted by proteins of host origin as well as some proteins that the parasite ‘exports’ to the host cell surface. Nearly 550 parasite proteins are thought to be exported to the infected erythrocyte. Amongst the exported proteins is P. falciparum heat shock protein 70-x (PfHsp70-x). Hsp70 proteins are known to maintain protein homeostasis. Thus, the export of PfHsp70-x may be important for maintaining protein homeostasis in the host cell. PfHsp70-x is not essential for parasite survival although is implicated in the development of parasite virulence. This is possibly through its role in facilitating the trafficking of parasite proteins to the erythrocyte as well as supporting the formation of protein complexes that constitute the knobs that develop on the surface of the infected erythrocyte. The main objective of the current study was to investigate protein interaction partners of PfHsp70-x. It is generally believed that PfHsp70-x interacts with various proteins of human and parasite origin. Potential candidate interactors include its protein substrates, Hsp70 co-chaperones such as Hsp40 members, and human Hsp70-Hsp90 organizing protein (hHop). The establishment of the PfHsp70-x interactome would highlight the possible role of PfHsp70-x in the development of malaria pathogenicity. Based on bioinformatics analysis, PfHsp70-x was predicted to interact with some exported P. falciparum Hsp40s, hHop and human Hsp90 (hHsp90). Recombinant forms of PfHsp70-x (full length and a truncated form that lacks the C-terminal EEVN motif implicated in co-chaperone binding) were expressed in E. coli BL21 Star (DE3) cells. Recombinant hHop and hHsp70 were expressed in E. coli JM109 (DE3) cells. The proteins were successfully purified using nickel affinity chromatography. Co-affinity chromatography using recombinant PfHsp70-x and immuno-affinity chromatography using PfHsp70-x specific antibody did not confirm the direct interaction of PfHsp70-x with human Hop. However, the direct interaction of hHop and PfHsp70-x has previously been validated in vitro and the current bioinformatics data support ii the existence of such a complex. PfHsp70-x was not stable in the cell lysate that was prepared and this could explain why its interaction with hHop could not be ascertained. However, taken together the evidence from a previous independent study, and the predicted interaction of PfHsp70-x with human chaperones suggests cooperation of chaperone systems which possibly facilitates the folding and function of parasite proteins that are exported to the infected erythrocyte.
NRF
39
Madisha, Mpho Christa Judith. "Factors altering HIV and Aids postnatal clients' commitment to exclusive breastfeeding." Diss., 2008. http://hdl.handle.net/10500/2952.
Abstract:
The study sought to explore and describe the breastfeeding practices of Human Immunodeficiency Virus (HIV) positive postnatal clients’ non-commitment to exclusive breastfeeding in George Mukhari Hospital, South Africa. A non-experimental quantitative design was used.
Inferences drawn from the study were that HIV positive clients that opted for exclusive breastfeeding did not commit for fear of transmission of HIV to the baby and exclusive breastfeeding was stopped before the recommended 6 months. Most of the respondents’ partners did not come for counselling. There was lack of emotional support by staff after testing. Health education and emotional support of HIV positive clients has to be intensified.Health Studies
M. A. (Health Studies)
40
Hijazi, Heba Hesham. "Factors affecting contraceptive use among women of reproductive age in northern Jordan : a framework for health policy action." Thesis, 2012. http://hdl.handle.net/1957/29089.
Abstract:
Jordan has a higher fertility rate (3.8) than the averages of countries similar in income to Jordan (2.2) and compared to the Middle East and North Africa region as a whole (2.8) (WHO, WB, UNICEF, & DHS, 2011). The findings of the 2009 Jordanian Population and Family Health Survey demonstrated that the total fertility rate (TFR) has stopped declining in the country since 2002 (DOS, 2010b; USAID, 2010). The prevalence of contraceptive use has also shown little change in Jordan over the last decade (DOS, 2010b; USAID, 2010). Given that contraception is one of the proximate determinants of fertility (Rahayu et al., 2009), the main purpose of this study was to investigate which factors are contributing to women's current contraceptive behavior and intention for future contraceptive use. Research questions were developed in a comprehensive
framework that considers women's intention and actual behavior as outcomes of various interactive factors within a socio-cultural context. In particular, the study's framework was directed by a theoretical basis adapted from Ajzen and Fishbein's Theory of Reasoned Action (TRA) and an extensive review of the available literature in the research area. Obviously, the social set-up and cultural norms in the study setting, together with attitudes toward children and family, represent a traditional scenario that could help explain the consistency of fertility and contraceptive use in the country. Further, the influences of background characteristics on women's contraceptive behaviors and intentions provide another scenario that could help assess the current situation of family planning (FP) in Jordan. In this study, demographic factors, spousal communication variables and healthcare system-related factors are all defined as background characteristics. Attitudes and social norms reflect the women's behavioral determinants and represent the main constructs of the TRA. In fact, involving a set of factors related to women's beliefs and social norms in the study's framework provided an opportunity to explore how these factors might promote or inhibit a woman's intentions and behaviors in respect to contraceptive use. In a three-manuscript format, this research was designed to achieve a number of objectives. The first manuscript aimed at identifying the major factors associated with the current use of contraception among women of childbearing age in northern Jordan. The second manuscript focused on investigating the main factors that are associated with women's contraceptive method preference (e.g. the choice of modern contraceptives as effective methods in preventing pregnancy versus the choice of traditional contraceptives as methods with high failure rates). The third manuscript attempted to explore the key factors associated with women's intention for
future contraceptive use since the existence of such an intention would consequently translate into an actual behavior later. In 2010, original cross-section data were collected by means of a face-to-face interview using a structured pre-tested survey. The study sample included women who were currently married and were between 18 and 49 years old. Applying a systematic random sampling procedure, all respondents were recruited from the waiting rooms of five randomly selected Maternal and Child Health (MCH) centers in the Governorate of Irbid, northern Jordan. Using a list provided by the Ministry of Health, all centers in the Governorate were stratified according to the region (urban vs. rural) and randomly selected in proportion to their number in each region. The final sample size for this research consisted of 536 women surveyed, giving a response rate of 92.4 percent. Utilizing logistic regression analyses, the results of the dissertation manuscripts indicate that women's behaviors and intentions toward the use of contraception are affected by a number of factors at the individual, familial and institutional levels. The findings that emerged from the three manuscripts provide health professionals and policy makers with important information to assist in the design of FP programs and campaigns aimed at increasing current contraceptive use, enhancing the adoption of modern contraception and motivating the intention for future contraceptive use. This research strongly suggests that health professionals develop health policies that both expand the availability of MCH centers and strengthen the role of healthcare providers to dispel the numerous rumors and misconceptions surrounding the use of contraceptives, particularly modern ones. Health workers at the MCH centers need to ensure that women have sufficient information about the benefits and side effects of different types of contraception by offering proper FP counseling. The messages that
religious leaders can use in advocating for FP would also help make contraceptive use socially acceptable since their opinions are often followed by the majority. This would be a key step toward removing the barriers to contraceptive use. Moreover, to design effective FP interventions, planners should take into account women's attitudes toward the use of contraceptive methods and the components of those attitudes (e.g. women's approval of contraceptive use for birth spacing and perceptions regarding the value of large family sizes and the importance of having male children in Jordanian families).Graduation date: 2012
Access restricted to the OSU Community at author's request from May 9, 2012 - May 9, 2013
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Yirdaw, Kesetebirhan Delele. "Prevalence and predictors of immunologic failure among HIV patients on HAART in southern Ethiopia." Diss., 2014. http://hdl.handle.net/10500/18970.
Abstract:
Immunologic monitoring is part of the standard care for patients on antiretroviral treatment. Yet, little is known about the routine implementation of immunologic monitoring in Ethiopia. This study assessed the pattern of immunologic monitoring, immunologic response, level of immunologic treatment failure and factors related to it among patients on antiretroviral therapy in selected hospitals in southern Ethiopia. A retrospective longitudinal analytic study was conducted using documents of patients started on antiretroviral therapy.
A total of 1,321 documents of patients reviewed revealed timely immunologic monitoring were inadequate. Despite overall adequate immunologic response, the prevalence of immunologic failure was 11.5% (n=147). Having WHO Stage III/IV of the disease and a higher CD4 (cluster differentiation 4) cell count at baseline were identified as risks for immunologic failure.
These findings highlight the magnitude of the problem of immunologic failure. Prioritizing monitoring for high risk patients may help in effective utilisation of meager resourcesHealth Studies
M.A. (Public Health)
42
Shwetank, *. "Infection of Human Cell Lines by Japanese Encephalitis Virus : Increased Expression and Release of HLA-E, a Non-classical HLA Molecule." Thesis, 2013. http://etd.iisc.ernet.in/2005/3457.
Abstract:
Japanese encephalitis virus (JEV) causes viral encephalitis in new born and young adults that is prevalent in different parts of India and other parts of South East Asia with an estimated 6000 deaths per year. JEV is a single stranded RNA virus that belongs to the Flavivirusgenus of the family Flaviviridae. It is a neurotropic virus which infects the central nervous system (CNS). The virus follows a zoonotic life-cycle involving mosquitoes and vertebrates, chiefly pigs and ardeid birds, as amplifying hosts. Humans are dead end hosts. After entry into the host following a mosquito bite, JEV infection leads to acute peripheral leukocytosis in the brain and damage to Blood Brain Barrier (BBB). The exact role of the endothelial cells during CNS infection is still unclear. However, disruption of this endothelial barrier has been shown to be an important step in entry of the virus into the brain.
Humoral and cell mediated immune responses during JEV infection have been intensively investigated. Previous studies from our lab have shown the activation of cytotoxic T-cells (CTLs) upon JEV infection. MHC molecules play pivotal role in eliciting both adaptive (T-cells) and innate (NK cells) immune response against viral invasion. Many viruses such as HIV, MCMV, HCMV, AdV and EBV have been found to decrease MHC expression upon infection. On the contrary, flaviviruses like West Nile Virus (WNV) have been found to increase MHC-I and MHC-II expression. More recently, data from our lab has shown that JEV infection can lead to upregulation of mouse non-classical MHC class Ib molecules like Qb1, Qa1 and T-10 along with classical MHC molecules.
Non-classical MHC molecules are important components of the innate and adaptive immune systems. Non-classical MHC molecules differ from their classical MHC class I counterparts by their limited polymorphism, restricted tissue distribution and lower levels of cell surface expression. Human classical MHC class I molecules are HLA-A, -B and –C while non-classical MHC Class Ib molecules are HLA-E, -G and –F. HLA-E, the human homologue of the mouse non-classical MHC molecule, Qa-1b has been shown to be the ligand for the inhibitory NK, NKG2A/CD94 and may bridge innate and adaptive immune responses.
In this thesis, we have studied the expression of human classical class I molecules HLA-A, -B, -C and the non-classical HLA molecule, HLA-E in immortalized human brain microvascular endothelial cells (HBMEC), human endothelial like cell line ECV304 (ECV), human glioblastoma cell line U87MG and human foreskin fibroblast cells (HFF). We observed an upregulation of classical HLA molecules and HLA-E mRNA in endothelial and fibroblast cells upon JEV infection. This mRNA increase also resulted in upregulation of cell surface classical HLA molecules and HLA-E in HFF cells but not in both the human endothelial cell lines, ECV and HBMECs.
Release of soluble classical HLA molecules upon cytokine treatment has been a long known phenomenon. Recently HLA-E has also been shown to be released as a 37 kDa protein from endothelial cells upon cytokine treatments. Our study suggests that JEV mediated upregulation of classical HLA and HLA-E upregulation leads to release of both Classical HLA molecules and HLA-E as soluble forms in the human endothelial cell lines, ECV and HBMEC. This shedding of sHLA-E from human endothelial cells was found to be mediated by matrix metalloproteinase (MMP) proteolytic activity. MMP-9, a protease implicated in release of sHLA molecules was also found to be upregulated upon JEV infection only in endothelial cell lines but not in HFF cells. Our study provides evidence that the JEV mediated solubilisation of HLA-E could be mediated by MMP-9. Further, we have tried to understand the role of the MAPK pathway and NF-κB pathway in the process of HLA-E solubilisation by using specific inhibitors of these pathways during JEV infection of ECV cells. Our data suggests that release of sHLA-E is dependent on p38 and JNK pathways while ERK 1/2 and NF-κB pathway only had a minor role to play in this process.
Treatment of endothelial cells with TNF-α, IL-1β and IFN-γ is known to result in release of sHLA-E. In addition to TNF-α and IFNtreatment, we observed that activating agents like poly (I:C), LPS and PMA also resulted in the shedding of sHLA-E from ECV as well as U87MG but not from HFF cells. Treatment of endothelial cells with IFN-β, a type-I interferon also led to release of sHLA-E. IFN-γ, a type II interferon and TNF-α are known to show additive increase in solubilisation of HLA-E. We studied the interaction between type I interferon, IFN-β and TNF-α with regard to shedding of sHLA-
E. Both IFNand TNF, when present together caused an additive increase in the shedding of sHLA-E. These two cytokines were also found to potentiate the HLA-E and MMP-9 mRNA expression. Hence, our data suggest that these two cytokines could be working conjunctly to release HLA-E, when these two cytokines are present together as in the case of virus infection of endothelial cells.
HLA-E is known to be a ligand for NKG2A/CD94 inhibitory receptors present on NK and a subset of T cells. Previous reports have suggested that NKG2A/CD94 mediated signaling events could inhibit ERK 1/2 phosphorylation leading to inhibition of NK cell activation. IL-2 mediated ERK 1/2 phosphorylation is known to play a very important role in maintenance and activation of NK cells. We studied the effects of sHLA-E that was released, either by JEV infection or IFN-γ treatment on IL-2 mediated ERK 1/2 phosphorylation in two NK cell lines, Nishi and NKL.
The soluble HLA-E that was released upon JEV infection was functionally active since it inhibited IL-2 and PMA induced phosphorylation of ERK 1/2 in NKL and Nishi cells. Virus infected or IFN-γ treated ECV cell culture supernatants containing sHLA-E was also found to partially inhibit IL-2 mediated induction of CD25 molecules on NKL cells. CD25 is a component of the high affinity IL-2 receptor and hence could play an important role in proliferation and activation of NK cells. sHLA-E was also found to inhibit IL-2 induced [3H]-thymidine incorporation suggesting that, similar to cell surface expressed HLA-E, sHLA-E could also inhibit the proliferation and activation of NK cells.
In summary, we found that establishment of JEV infection and production of cytokines like IFN-β, TNF-α, IL-6 along with MMP-9 in human endothelial cells. These cytokines may also indirectly lead to the reported damage and leukocyte infiltration across infected and uninfected vicinal endothelial cells. The increased surface expression of HLA-E in fibroblast and release of sHLA and sHLA-E molecules from endothelial cells may have an important immunoregulatory role. HLA-E is an inhibitory ligand for NKG2A/CD94 positive CD8+ T and NK cells. Hence our finding that sHLA-E can inhibit NK cell proliferation suggests an immune evasive strategy by JEV.
43
Plaatjie, Bulelwa. "The impact of HIV and AIDS on planned parenthood in the area of Mthatha." Diss., 2009. http://hdl.handle.net/10500/3092.