Relevant bibliographies by topics / Female identity

Academic literature on the topic 'Female identity'

Author: Grafiati
Published: 4 June 2021
Last updated: 21 February 2023

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Journal articles on the topic "Female identity"

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Tiwari, Prisha. "The Female Quest for Identity." Motifs : An International Journal of English Studies 4, no. 1and2 (2018): 18. http://dx.doi.org/10.5958/2454-1753.2018.00005.3.

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Robinson, G. Erlick, and Donna E. Stewart. "Female Sexuality and Identity: Introduction." Canadian Journal of Psychiatry 34, no. 9 (December 1989): 860. http://dx.doi.org/10.1177/070674378903400903.

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Smith-Rosenberg, Carroll. "Subject Female: Authorizing American Identity." American Literary History 5, no. 3 (1993): 481–511. http://dx.doi.org/10.1093/alh/5.3.481.

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Geiger, Brenda, and Michael Fischer. "Female Repeat Offenders Negotiating Identity." International Journal of Offender Therapy and Comparative Criminology 47, no. 5 (October 2003): 496–515. http://dx.doi.org/10.1177/0306624x03253025.

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Babana-Hampton, Safoi. "Literary Representations of Female Identity." American Journal of Islam and Society 19, no. 4 (October 1, 2002): 23–41. http://dx.doi.org/10.35632/ajis.v19i4.1914.

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The essay examines the texts of the two women writers - Leila Abouzeid (from Morocco) and Nawal El Saadawi (from Egypt) - as offering two female perspectives within what is commonly referred to as "feminine" writing in the Arab Muslim world. My main interest is to explore the various discursive articulations of female identity that are challenged or foregrounded as a positive model. The essay points to the serious pitfalls of some feminist narratives in Arab-Muslim societies by dealing with a related problem: the author's setting up of convenient conceptual dichotomies, which account for the female experience, that reduce male-female relationships in the given social context to a fundamentally antagonistic one. Abouzeid's novel will be a case study of a more positive but also realistic and complex perspec­tive on female experience ...
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Aengenheyster, Johannes, and Caitlin Masoliver. "MODERN CATHOLICISM AND FEMALE IDENTITY." Maastricht Journal of Liberal Arts 9 (June 27, 2017): 49. http://dx.doi.org/10.26481/mjla.2017.v9.456.

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Haig, Philip M. "Female Stories, Female Bodies: Narrative, Identity, and Representation (review)." symploke 6, no. 1 (1998): 209–10. http://dx.doi.org/10.1353/sym.2005.0075.

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Kosmala, Katarzyna. "The Identity Paradox? Reflections on Fluid Identity of Female Artist." Culture and Organization 13, no. 1 (March 2007): 37–53. http://dx.doi.org/10.1080/14759550601167271.

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Gogová, Lenka. "Representation of female identity in humour." Ars Aeterna 14, no. 1 (June 1, 2022): 36–49. http://dx.doi.org/10.2478/aa-2022-0004.

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Abstract The article discusses humour as a form of communication and social interaction, which is not only based on sociocultural similarities, tolerance and solidarity among in-group members but also hostility or aggression towards out-group members. As humour is formed on binary oppositions, the female gender is often used as a popular “target” in humour discourse. It also represents “otherness” regarding the opposite gender and communicates social codes based on physical appearance, behaviour, or specific roles in society. Gender-stereotyping, which is used to categorize and understand the “outside” world better, is one of the most common and simplest approaches in humour discourse. The main aim of our research is to discuss the role of women and the way female identity, as a social construct, is defined and presented in humour discourse through stereotypes. More precisely, this article examines the evolution of women’s representation in the situation comedies with regards to their stereotypical portrayals and traditional social roles.
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Spencer, Jenny. "Norman's'night, Mother:Psycho-drama of Female Identity." Modern Drama 30, no. 3 (September 1987): 364–75. http://dx.doi.org/10.3138/md.30.3.364.

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Dissertations / Theses on the topic "Female identity"

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Tsaousi, Christiana. "Consuming underwear : fashioning female identity." Thesis, University of Leicester, 2011. http://hdl.handle.net/2381/9393.

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In academic literature underwear is a largely neglected part of women’s clothing which, this thesis argues, is nonetheless as important as ‘outward’ dress itself. Indeed in some ways underwear is more interesting in the sense that it is hidden from view but still appears to have considerable social/discursive importance. The thesis suggests that underwear functions as a source for (re)constructing female identity and that women ‘learn’ through their embodied experience of choosing the ‘right’ underwear for the right occasion to fashion elements of their identity accordingly. Using a conceptual combination of work by Michel Foucault, Pierre Bourdieu and selected feminists, alongside theories of consumption, the thesis argues that underwear can be seen as a technology of the self and as embodied cultural capital. It functions both as a support for outerwear and the body, and as a tool for self-fashioning and self-improvement due to the intense sensations it can produce for the wearer. Using a series of focus groups and interviews, based on the concept of identity opseis which reflects the different sides of identity a woman arguably plays out in her everyday life, the thesis aims to contribute to the field of the sociology of consumption by exploring the role of socio-cultural imperatives and of taste in the consumption of women’s underwear. The empirical data indicate that underwear is used for the construction and reconstruction of various feminine identities, including worker, mother, sports player and sexual partner. It analyses the importance respondents attribute to underwear according to whether it is hidden or visible; the physical/psychological sensations it induces for the respondents; the varying mobilisations of underwear to support aspects of the female identity project; the role of taste when choosing underwear; and the experiences the respondents report regarding shopping for underwear. Thus this thesis contributes to the limited scholarly literature on underwear and establishes an understanding of how such mundane forms of body work can be elements of constructing women’s ongoing and complex identity projects.
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Ball, Victoria. "Female identity and the British female ensemble drama 1995-1998." Thesis, Queen Margaret University, 2007. https://eresearch.qmu.ac.uk/handle/20.500.12289/7347.

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This thesis focuses upon a distinctive form of ‘feminine-gendered’ fiction, that of the British female ensemble drama, that has proliferated across televisual schedules since the late 1970s and which has received little academic attention. Although not a discrete genre, the female ensemble drama is nevertheless identifiable as a distinctive form of ‘feminine-gendered’ fiction that is largely written and/or produced by women, which diegetically focuses on particular communities of female characters and which is predominantly aimed at female audiences. The purpose of this text-based analysis of the female ensemble drama is to engage with a central concern of feminist television criticism, that of the gendered identity of this particular media form and the constructions of gender within it given its association with women at these three sites of production, text and audience. While I provide a historical overview of the development of this form of drama in relation to its textual precedents I isolate a particular moment in the history of this form of drama, that of the late 1990s, for closer analysis. Firstly I isolate the late 1990s to provide knowledge and understanding of the way in which the ‘feminine’ identity of this form of drama has contributed to its academic neglect within this socio-cultural period. Secondly I provide a close textual analysis of the constructions of ‘women’ within three female ensemble dramas in order to engage with and explore the textual negotiations they embody surrounding discourses of feminism and post feminism, de- and re-traditionalization in this particular period. While these themes have begun to be addressed in feminist television criticism they have largely been explored in relation to constructions of femininity in American dramas. This analysis then, allows for an exploration of these discourses in relation to a regional form of British drama. It is through investigating the academic neglect of this form of drama; providing a historical, thematic and aesthetic overview of the female ensemble drama as well as a detailed analysis of three of the female ensemble dramas of the 1990s that I contribute knowledge and understanding of this particular regional form of ‘feminine-gendered’ fiction to the field of Feminist Television Studies.
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Berndt, Katrin. "Female identity in contemporary Zimbabwean fiction /." Bayreuth : Thielmann & Breitinger, 2005. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=013041976&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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Munir, Shaheen Sikander. "Identity and anxiety among female adults /." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487597424135029.

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Walters, Melanie L. "Mother/daughter dyads : female identity construction in three contemporary female Bildungsromane /." Available to subscribers only, 2008. http://proquest.umi.com/pqdweb?did=1559850931&sid=10&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Blackmon, Carlotta M. "Routed Sisterhood: Black American Female Identity and the Black Female Community." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1238090994.

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Van, Eijck Jo-Ann. "Constructing contemporary Cuban female identity : female traces in the visual arts." Thesis, SOAS, University of London, 2004. http://eprints.soas.ac.uk/29334/.

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This study will assess five contemporary female artists to explore ways in which their art production can be meaningfully read in relation to their sense of being Cuban and what this might mean for them at this juncture in Cuba's history. The first three chapters introduce the artists - Maria Magdalena Campos-Pons, Marta Maria Perez, Belkis Ayon, Tania Bruguera and Sandra Ramos - and the unique Cuban visual arts spectrum post-1980, vis-a-vis movements, people, ideology, education and the impact of socialist influences. Key factors regarding Cuban identity will also be examined; gender, race, socio-cultural and religious practices, as these elements have been fundamental to the self-conscious identity constructions of these women through their art. As products of the revolutionary process, artists have delivered sophisticated avant-garde high art creations that embody the worldviews of the Cuban people. And, as professional artists, they are afforded specific ideological, ethical and social responsibilities and privileges within Cuban society. Their creative endeavours have become much-needed critical spaces to comment when other Cubans cannot and to consider issues of specific relevance to their country. Drawing on the resources of iconography and various semiotic devices, the following three chapters focus on these women's lives and artistic trajectories via the topics they address, such as myth, religion, displacement and the Cuban Diaspora. As a recurrent element in their work and one historically connected to the Cuban visual arts tradition and notions of identity, their portrayals of the female body will be read as sites for socio-cultural, personal and ideological discourse within the parameters of the contemporary socialist Cuban framework. Also, the nature of the plastic arts medium and the possibilities inherent in being a Cuban artist will be examined, and the other 'bodies' present in their work; the body of the audience and the body of the artwork.
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Prince, Jane. "Processes of identity in female police officers." Thesis, Cranfield University, 1993. http://dspace.lib.cranfield.ac.uk/handle/1826/8753.

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Almost all studies of policewomen are concerned with strong and specific strains on the identity. The literature reviewed reveals both two social divides and two occupational divides. Theories of identity are also reviewed and these similarly separate into those concerned with socialisation and others which focus on struggles, on conflicts. Both draw attention to the social contexts and to coping strategies. From these literature based accounts a methodology is derived which brings together the quantitative and the qualitative through the use of the survey, the interview and participant observation. There were 152 respondents, 24 interview subjects and three periods of observation. The key link is to be found in the Theory of Type. There is a policewomen personality which extraverts 'sensing'; external world patterns and facts are preferred to abstract relationships. This type is in balance with the background characteristics identified. Family members encouraged joining, educational levels are higher than average and the women joined especially for job security and pay along with a value of public service. Their dislikes spread across their treatment by both men and the management structure. Their likes are for the variety and unpredictability of the work itself. The critical incident interviews deepen the understanding of the conflicts experienced, five major conflicts being identified. The responses include confrontation, a strategy previously unidentified as having the same degree of Significance as others in managing conflicts of identity. Passing and denial are much less frequently used. The longer serving and the promoted women are more likely to be confrontational in their responses to contradictions. The distinction between policewomen and policewomen was not identifiable in this sample. The greatest preference for managing contradictions and conflict was through assertion and confrontation. These data lead to the conclusion that gender identity can be a synthesis rather than a segmentation. Furthermore this synthesis may be both personal and stable and an aspect of policewomen whether on or off duty.
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Okan, Olgaokan. "Narrative constructions of female identity after suicide." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7419/.

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This thesis weaves together two central themes in the analysis of literary suicide: writing and gender. In particular, it looks at different interpretations of the suicides of Eleanor Marx, Virginia Woolf, Sylvia Plath and Sarah Kane. Apart from being writers who committed suicide, these women share a common interest in suicide as a subject matter in their writings. Especially in the cases of Woolf and Plath, their iconic status as literary suicides has often blurred the distinction between fact and fiction in the studies of their life and work. Furthermore, they have become case studies in the fields of psychology/psychiatry which discuss creativity in relation to mental illness. In this thesis, I take into account the connotations of literary suicide in different fields of study and synthesize an interdisciplinary approach with a focus on gender. Drawing on Judith Butler’s theory of performativity and Katrina Jaworski’s adaptation of it to suicide, I explore suicide as a social and historical construct. The thesis traces the subject formation of suicide through textual analysis of primary sources (including fiction, biographies and print media) and considers suicide notes, newspaper reports, obituaries and letters as the first narrative constructions of suicidal identity. Initial reactions to these suicides show a highly gendered understanding. However, the multiple narratives that follow reflect changes in the discourse of suicide. The thesis analyses the narratives of suicide written by the authors in relation to dominant discourses of suicide, the self and gender. The examination of the writers’ own work demonstrates that Marx, Woolf, Plath and Kane were in most cases writing against the dominant discourses of suicide.
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Juliussen-Stevenson, Heather Ann. "Performing christian female identity in Roman Alexandria." College Park, Md. : University of Maryland, 2008. http://hdl.handle.net/1903/8220.

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Thesis (M.A.) -- University of Maryland, College Park, 2008.
Thesis research directed by: Dept. of History. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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Books on the topic "Female identity"

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Female stories, female bodies: Narrative, identity, and representation. Washington Square, N.Y: New York University Press, 1998.

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Curti, Lidia. Female stories, female bodies: Narrative, identity, and representation. Houndmill, Basingstoke: Macmillan Press, 1998.

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Female identity in contemporary Zimbabwean fiction. Bayreuth: Eckhard Breitinger, Bayreuth University, 2005.

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Basu, Shrabani. Gendered Identity and the Lost Female. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-4967-8.

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Norbert, Freedman, and Distler Betsy, eds. Female identity conflict in clinical practice. Northvale, N.J: Jason Aronson, Inc., 1993.

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Halberstam, Judith. Female masculinity. Durham: Duke University Press, 1998.

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Reconceiving women: Separating motherhood from female identity. New York: Guilford Press, 1993.

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Hovet, Grace Ann. Tableaux Vivants: Female Identity Development through Everyday Perfomance. Bloomington, IN]: Xlibris Corporation, 2009.

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1944-, Enders Victoria Lorée, and Radcliff Pamela Beth, eds. Constructing Spanish womanhood: Female identity in modern Spain. Albany, N.Y: State Univesity of New York Press, 1999.

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Picchietti, Virginia, and Laura A. Salsini, eds. Writing and Performing Female Identity in Italian Culture. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-40835-4.

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Book chapters on the topic "Female identity"

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Borgeson, Kevin, and Robin Maria Valeri. "Female Skinheads." In Skinhead History, Identity, and Culture, 66–90. 1 Edition. | New York: Routledge, 2018. |: Routledge, 2017. http://dx.doi.org/10.4324/9781315474816-4.

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Hogarth, Christopher. "Language and Afropean Identity." In Afropean Female Selves, 107–33. New York: Routledge, 2022. http://dx.doi.org/10.4324/9781003203858-5.

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Marshall, Annecka, and Donna-Maria Maynard. "Black Female Sexual Identity." In Black Genders and Sexualities, 195–202. New York: Palgrave Macmillan US, 2012. http://dx.doi.org/10.1057/9781137077950_13.

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Negrin, Llewellyn. "Cosmetics and the Female Body." In Appearance and Identity, 53–74. New York: Palgrave Macmillan US, 2008. http://dx.doi.org/10.1057/9780230617186_4.

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Coward, Rosalind. "Female Desire and Sexual Identity." In Critical Theory, 25. Amsterdam: John Benjamins Publishing Company, 1985. http://dx.doi.org/10.1075/ct.1.05cow.

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Jones, Tiffany, Andrea del Pozo de Bolger, Tinashe Dune, Amy Lykins, and Gail Hawkes. "Gender Identity." In Female-to-Male (FtM) Transgender People’s Experiences in Australia, 33–43. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13829-9_4.

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Steveker, Lena. "Female Autonomy." In Identity and Cultural Memory in the Fiction of A. S. Byatt, 55–64. London: Palgrave Macmillan UK, 2009. http://dx.doi.org/10.1057/9780230248595_6.

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Purkis, Judy. "The Quintessential Female Act? Learning about Birth." In Gender, Identity & Reproduction, 103–19. London: Palgrave Macmillan UK, 2003. http://dx.doi.org/10.1057/9780230522930_7.

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Eroğul, Murat Şakir. "Constructing Female Entrepreneurial Identity in Turkey." In A Comparative Perspective of Women’s Economic Empowerment, 200–218. 1 Edition. | New York : Routledge, 2019. |: Routledge, 2019. http://dx.doi.org/10.4324/9780429053146-12.

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Coltri, Marzia A. "Women and NRMs: Location and Identity." In Female Leaders in New Religious Movements, 11–28. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-61527-1_2.

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Conference papers on the topic "Female identity"

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"Female Identity or Power Game? -- Comment on Raziya, Female Sultan of Medieval India." In 2017 International Conference on Social Sciences, Arts and Humanities. Francis Academic Press, 2017. http://dx.doi.org/10.25236/ssah.2017.59.

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Robinson, Petra. "Critical Media Literacy and Black Female Identity Construction." In 2021 AERA Annual Meeting. Washington DC: AERA, 2021. http://dx.doi.org/10.3102/1691790.

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Chen, Xin, and Ying-Ling Liu. "An Analysis of Female Identity Construction in English Advertisements." In International Academic Conference on Frontiers in Social Sciences and Management Innovation (IAFSM 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200207.056.

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Ramadhan, Risca Ariska. "Rimpu and Symbolization of Female Identity in Bima Community." In Fourth Prasasti International Seminar on Linguistics (Prasasti 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/prasasti-18.2018.51.

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LIOU, SHYH NAN, and SZU YIN LI. "Gender Identity of Androgynous Female A Perspective of Intersubjectivity." In Second International Conference on Advances in Management, Economics and Social Science - MES 2015. Institute of Research Engineers and Doctors, 2015. http://dx.doi.org/10.15224/978-1-63248-046-0-116.

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Burrell-Craft, Kala. "A Conceptual Framework for Positive Black Female Identity Formation." In 2021 AERA Annual Meeting. Washington DC: AERA, 2021. http://dx.doi.org/10.3102/1692168.

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Shane-Nichols, Amy, Ann Marie Marie Fiore, and Mary Lynn Damhorst. "Female Consumers’ Symbolic Expression of Identity through Harley-Davidson Apparel." In Pivoting for the Pandemic. Iowa State University Digital Press, 2020. http://dx.doi.org/10.31274/itaa.11793.

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Xu, Huijie. "Female Identity and Construction in Fashion—Based on Simmel’s fashion philosophy." In proceedings of the 2nd International Conference on Literature, Art and Human Development (ICLAHD 2020). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.201215.437.

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Serrano, Mayari I., and Jennifer L. Groh. "Travel grants which facilitate engineering leadership identity in female engineering students." In 2016 IEEE Frontiers in Education Conference (FIE). IEEE, 2016. http://dx.doi.org/10.1109/fie.2016.7757642.

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Muhtar, Sitti Murniati, Muh Iqbal Sultan, Andi Subhan Amir, and Sitti Nurfajriani Syam. "Hair and Female Identity: Reading Women's Hair in The Mass Media." In Unhas International Conference on Social and Political Science (UICoSP 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/uicosp-17.2017.29.

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Reports on the topic "Female identity"

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Lee, Youngji, Nancy J. Hodges, and Seoha Min. �55 Is Not Old!�: Aging and Identity in Fashion Blogs Targeting Female Baby Boomers. Ames (Iowa): Iowa State University. Library, January 2019. http://dx.doi.org/10.31274/itaa.8392.

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Albrecht, Milde, Bertha Jacobs, and Arda Retief. The influence of important values and predominant identity on South African female Muslim students’ dress practices. Ames: Iowa State University, Digital Repository, 2013. http://dx.doi.org/10.31274/itaa_proceedings-180814-798.

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Lacalle, Ch, and D. Castro. Self-identity disclosure in TV Fandom. Analysing the comments posted by Spanish female fans and community managers. Revista Latina de Comunicación Social, January 2018. http://dx.doi.org/10.4185/rlcs-2017-1242en.

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Lacalle, Ch, and D. Castro. Self-identity disclosure in TV Fandom. Analysing the comments posted by Spanish female fans and community managers. Revista Latina de Comunicación Social, January 2017. http://dx.doi.org/10.4185/rlcs-2018-1242en.

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Morris, Kristen D., Michelle Teti, Cole Young, and Abigail Rolbiecki. Photovoice: A user-centered design method to understand apparel needs of Female to Male (FTM) in gender identity and expression. Ames: Iowa State University, Digital Repository, 2017. http://dx.doi.org/10.31274/itaa_proceedings-180814-427.

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Mitchell Dove, Lakindra. Got Hair that Flows in the Wind: The Complexity of Hair and Identity among African American Female Adolescents in Foster Care. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2318.

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Thomas, Jakana. Duty and Defiance: Women in Community-based Armed Groups in West Africa. RESOLVE Network, March 2021. http://dx.doi.org/10.37805/cbags2021.1.

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This desk report explores how West African community-based armed groups (CBAGs) facilitate women’s engagement with politics, create avenues for female expressions of anger, commitment to community values and national identity, and enable women to push for change in their communities by opening spaces for female participation. Assessing the formal and informal contributions women make to armed community mobilization and hybrid security reveals opportunities for gender-specific engagement and cautions that unidimensional considerations of where and how women intersect with conflict and security have the potential to undermine violence reduction and post-conflict peacebuilding efforts.
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Frisancho, Verónica, Alejandro Herrera, and Eduardo Nakasone. Does Gender and Sexual Diversity Lead to Greater Conflict in the School? Inter-American Development Bank, September 2022. http://dx.doi.org/10.18235/0004451.

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This paper analyzes the relationship between the presence of LGBTQI students in the class-room and the prevalence of violence in the school setting. We rely on a representative sample of secondary schools in Uruguay and exploit variation in the share of LGBTQI students across classrooms to study how their presence affects the individual experience of violence. Our results show little support for the contact hypothesis: a larger share of LGBTQI students in the classroom has no impact on the individual experience of violence. On the contrary, a greater share of female LGBTQI students in the classroom is associated with greater psychological and physical violence among girls, irrespective of their gender identity or sexual orientation.
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Hicks, Jacqueline. The Role of Gender in Serious and Organised/Transnational Crime. Institute of Development Studies, March 2021. http://dx.doi.org/10.19088/k4d.2021.059.

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This rapid review synthesises evidence on the role of gender in serious and organised/transnational crime (SOC) with regard to gender norms, participation and prevention. It looks at the literature on the roles women play in organised crime groups and their pathways to participation, the impact of cultural gender norms in different forms of participation for men and women in SOC, and the role of gender dynamics within families or communities in preventing SOC. Key Overall Findings linking gender norms, female participation and prevention of SOC: 1). Gender norms and women’s participation in SOC are varied and highly contextual, highlighting the importance of gender analysis to programming; 2). Gendered perceptions of men as perpetrators and women as victims in SOC undermine effective responses; and 3). Some types of masculine identity have been linked to involvement in violent crime and societal tolerance of organised crime groups. In Italy, some feminists characterise opposition to SOC as an anti-patriarchal struggle.
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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.
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