Journal articles: 'Fasciola'

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Ustinov, A. M., R. T. Safiullin, and R. R. Safiullin. "Methodical guidelines on the control of fasciolosis in cattle in the Kaluga region." Russian Journal of Parasitology 12, no. 2 (June 28, 2018): 108–16. http://dx.doi.org/10.31016/1998-8435-2018-12-2-108-116.

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Information is provided on the causative agents of fasciolosis and the developmental biology of Fasciola hepatica. Fasciolosis of cattle is widespread in the conditions of the Kaluga Region and the Central Federal District on farms of different forms of ownership. The greatest number of F. hepatica eggs in 1 g of feces in cattle is established in JSC "Iskra" - 175,4 specimens, the lowest - in the samples from cows from private farms - 87,6 sp. At the opening slaughter of cattle at meat processing plants region and slaughterhouses invasion of their fasciolae was 34.8%. The average number fascial one infected animal was 32.1 specimens, including adult - and larval specimens of 25.2 and 6.9 copies At veterinary-sanitary expertise and helminthological autopsy of the liver, gallbladder and bile ducts installed year-round invasion fasciolae mainly in the adult stage. Young animals of the current year are infected with fasciola in the pasture massly in August-September, and the extent of invasion in November was 4.3%, for winter-stabling months an average of 12.6%, in March - 17.9% and in April after de-worming - 5.9%.The data obtained in the study of the distribution of the small pond has shown that in the Kaluga region these mollusks are widespread. In this case, the invasion of the small pond snail by larval forms of fasciolae in different years has its own peculiarities, which are influenced by climatic factors.The different dosage forms of clozantel - Rolenol and Santel and Genamectin with mixed fascioliasis and hypodermatosis of bovine in the recommended dose against subcutaneous gadfly larvae - provided 100% efficacy. The tested dosage forms of clozantel are similar in effectiveness against subcutaneous gadfly larvae (EE-100%) and fasciol (EE-95 and 90%, IE-97.4 and 96.3%).The therapeutic and economic effectiveness of complex domestic preparations of Helmicide and Fascocida in spontaneous fascioliasis of cattle is studied.

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Munawaroh, Alisa Qudrotun, Endry Nugroho Prasetyo, and Maharani Pertiwi Koentjoro. "A literature review: The comparison of the sedimentation and flotation technique for Fasciola hepatica detection in livestok." Jurnal Litbang Edusaintech 2, no. 2 (December 31, 2021): 79–85. http://dx.doi.org/10.51402/jle.v2i2.21.

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Fascioliasis is an infection of the trematodes of Fasciola hepatica and Fasciola gigantica which causes disease worldwide. This trematode infection commonly attacks ruminants, especially in cattle, buffalo, sheep, goats, pigs and humans, as well. This disease can cause significant economic losses in the livestock industry, mainly through death, liver damage, reduced production of meat, milk and wool, and expenses for deworming. Parasitological examination for identification of fasciola infection was carried out by visually observing fecal samples for adult worms and then examined by simple flotation (using saturated NaCl solution) and formalin-ethyl acetate sedimentation techniques. Here, we were systematically reviewed existing knowledge about sensitive and efficient fasciola observation methods for the identification of fasciola species. Four databases of articles that met the inclusion requirements were compiled for the literature review. This article was published between 2015-2020 and reports on the identification of fasciola species using one or a combination of sedimentation and flotation methods. As a result, the combined method of sedimentation and flotation has the best sensitivity and egg recovery rate (Ef) values for fasciola identification. Abstrak Fascioliasis merupakan infeksi trematoda spesies Fasciola hepatica dan Fasciola gigantica yang menimbulkan penyakit di seluruh dunia. Infeksi trematoda ini umum menyerang ruminansia, terutama pada sapi, kerbau, domba, kambing, babi dan bisa terjadi pada manusia. Penyakit ini dapat menimbulkan kerugian ekonomi yang cukup besar dalam industri ternak, terutama melalui kematian, kerusakan hati, berkurangnya produksi daging, susu, dan wol, dan pengeluaran biaya untuk obat cacing. Pemeriksaan parasitologi untuk identifikasi infeksi fasciola dilakukan dengan mengamati sampel feses secara visual untuk cacing dewasa dan kemudian diperiksa dengan cara flotasi sederhana (menggunakan larutan NaCl jenuh) dan teknik sedimentasi formalin-etil asetat. Disini, kami meninjau secara sistematis pengetahuan yang ada tentang metode pengamatan fasciola yang sensitif dan efisien dalam identifikasi spesies fascioal. Empat data base artikel yang telah memenuhi syarat inklusi disusun untuk tinjauan literature review. Artikel ini terbit antara tahun 2015-2020 dan melaporkan identifikasi spesies fasciola menggunakan salah satu atau gabungan metode sedimentasi dan flotasi. Hasilnya, metode gabungan antara sedimentasi dan flotasi memiliki nilai sensitivitas dan nilai tingkat pemulihan telur (egg recovery rate, Ef) paling baik untuk identifikasi fasciola.

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Omran, EmanK, and NohaS Ahmad. "Effect of nitroxynil (fasciolid) on adult Fasciola gigantica and Fasciola hepatica in infected cows." Parasitologists United Journal 8, no. 2 (2015): 107. http://dx.doi.org/10.4103/1687-7942.175008.

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Karsakov, Ataev, Zubairova, and Ataeva. "FEATURES OF TREMATODE INFECTION IN PASTURE OF DIFFERENT TYPES OF LOWLAND DAGESTAN." THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL, no. 21 (May 29, 2020): 128–32. http://dx.doi.org/10.31016/978-5-9902341-5-4.2020.21.128-132.

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In the lowland of Dagestan, sheep are infected with 5 species of trematodes – Fasciola hepatica (L., 1758), Fasciola gigantica (Cobbold, 1856), Dicrocoelium lanceatum (Stiles et Hassal, 1896), Paramphistomum cervi (Zeder, 1990), Calicophoron calicophorum (Fischoeder, 1901). The total infection of sheep with trematodas varies the extensity of invasion (EI) 56.0–76.0%, with the intensity of invasion (II) 2–2360 species. The sheep are intensely infected with fascioles on low-lying wetlands, with paramphistomatids on pastures of the lower reaches of the Sulak, Terek, Talovka, lakes Temirgoe, Achikol, and with Dicrocoelium in areas where flat steppes. Sheep are limited to trematodes invaded by salt marshes and desert lands, EI 1.6–6.6%, II 2–28 species. Sheep are intensively infected with these pathogens in the spring-summer-autumn time, when intermediate, additional hosts and the formation of parthenogenetic stages in them to adoleskarii, metacercarii are active in the biotopes between the rivers Sulak and Terek. Infection with Adolescaria fascial and Paramphistomum occurs in winter when they are snowless. Adolescaria are formed on water plants, on water surface, near sources of water. The lush greenery around the watering holes always attracts animals. In these areas, there is always a high risk of infection of sheep with fascioles, paramphistomatids.

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5

Simsek, S., A. Utuk, and I. Balkaya. "Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica." Helminthologia 48, no. 1 (March 1, 2011): 3–7. http://dx.doi.org/10.2478/s11687-011-0001-y.

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AbstractThe most common and widespread liver flukes of the genus Fasciola are Fasciola hepatica and F. gigantica. Adults of both species occur in many domestic ruminants and in humans and can cause serious disease. The differential diagnosis of these flukes infection is very important because of their different transmission and epidemiological characteristics. A simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the common restriction enzymes AluI and RsaI, is described to distinguish between both fasciolid species. After the digestion of the mitochondrial cytochrome c oxidase 1 (CO1) PCR product with the restriction enzyme AluI, the RFLP profile obtained from F. hepatica revealed two fragments, whereas F. gigantica was not cut. The RsaI digestion generated two fragments from F. gigantica, whereas it did not cut the PCR product from F. hepatica. Results were confirmed with CO1 sequence analysis of both F. hepatica and F. gigantica. The present study suggests that the PCRRFLP method described here can be used for the proper identification of Fasciola species.

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Afshan, K., M. A. Valero, M. Qayyum, R. V. Peixoto, A. Magraner, and S. Mas-Coma. "Phenotypes of intermediate forms of Fasciola hepatica and F. gigantica in buffaloes from Central Punjab, Pakistan." Journal of Helminthology 88, no. 4 (June 4, 2013): 417–26. http://dx.doi.org/10.1017/s0022149x13000369.

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AbstractFascioliasis is an important food-borne parasitic disease caused by the two trematode species, Fasciola hepatica and Fasciola gigantica. The phenotypic features of fasciolid adults and eggs infecting buffaloes inhabiting the Central Punjab area, Pakistan, have been studied to characterize fasciolid populations involved. Morphometric analyses were made with a computer image analysis system (CIAS) applied on the basis of standardized measurements. Since it is the first study of this kind undertaken in Pakistan, the results are compared to pure fasciolid populations: (a) F. hepatica from the European Mediterranean area; and (b) F. gigantica from Burkina Faso; i.e. geographical areas where both species do not co-exist. Only parasites obtained from bovines were used. The multivariate analysis showed that the characteristics, including egg morphometrics, of fasciolids from Central Punjab, Pakistan, are between F. hepatica and F. gigantica standard populations. Similarly, the morphometric measurements of fasciolid eggs from Central Punjab are also between F. hepatica and F. gigantica standard populations. These results demonstrate the existence of fasciolid intermediate forms in endemic areas in Pakistan.

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Molano Cetina, Linda Grace. "Fasciola hepatica." Biomédica 31, sup3.1 (September 30, 2011): 172. http://dx.doi.org/10.7705/biomedica.v31i0.549.

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8

Wang, M., and D. Pleskow. "Fasciola hepatica." Endoscopy 45, S 02 (July 25, 2013): E207—E208. http://dx.doi.org/10.1055/s-0033-1344063.

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9

Recco, Paulette. "Fasciola hepatica." EMC - Biologie Médicale 1, no. 1 (January 2006): 1–4. http://dx.doi.org/10.1016/s2211-9698(06)76275-6.

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10

Ahmad, Alzahraa Abdelraouf, Haidi Karam-Allah Ramadan, Waleed Attia Hassan, Mohammed Ageeli Hakami, Enas Abdelhameed Mahmoud Huseein, Sara Abdel-Aal Mohamed, Adnan Ahmed Mohamed, and Nahed Ahmed Elossily. "New perspectives for fascioliasis in Upper Egypt’s new endemic region: Sociodemographic characteristics and phylogenetic analysis of Fasciola in humans, animals, and lymnaeid vectors." PLOS Neglected Tropical Diseases 16, no. 12 (December 28, 2022): e0011000. http://dx.doi.org/10.1371/journal.pntd.0011000.

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Background Fascioliasis is a significant vector-borne disease that has emerged in numerous tropical and subtropical countries causing severe health problems. Egypt is one of the fascioliasis endemic regions; however, the current situation in Upper Egypt is understudied, with only sporadic human cases or outbreaks. This study aims to highlight the sociodemographic characteristics of human fascioliasis in a newly emerged endemic area in Upper Egypt, along with risk factors analysis and the molecular characteristics of the fasciolid population in humans, animals, and lymnaeid snails. Methodology/Principal findings The study reported Fasciola infection in patients and their close relatives by analyzing the risk of human infection. Morphological and molecular characterization was performed on lymnaeid snails. Multigene sequencing was also used to characterize fasciolids from human cases, cattle, and pooled snail samples. The study identified asymptomatic Fasciola infection among family members and identified the presence of peridomestic animals as a significant risk factor for infection. This is the first genetic evidence that Radix auricularia exists as the snail intermediate host in Egypt. Conclusions/Significance This study revealed that Assiut Governorate in Upper Egypt is a high-risk area for human fascioliasis that requires additional control measures. Fasciola hepatica was the main causative agent infecting humans and snail vectors in this newly emerged endemic area. In addition, this is the first report of R. auricularia as the snail intermediate host transmitting fascioliasis in Upper Egypt. Further research is required to clarify the widespread distribution of Fasciola in Egypt’s various animal hosts. This provides insight into the mode of transmission, epidemiological criteria, and genetic diversity of fasciolid populations in Upper Egypt.\

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11

Saadatnia, Aminallah, Kavous Solhjoo, Mohamad Hasan Davami, Saber Raeghi, and Ahmad Abolghazi. "Molecular Identification of Fasciola Isolated from the Liver of Meat Animals in Fars Province, Iran." Journal of Parasitology Research 2022 (March 26, 2022): 1–5. http://dx.doi.org/10.1155/2022/4291230.

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Background. Fasciola hepatica and Fasciola gigantica are flatworms that infect animals and humans. Fasciola is the parasite of the liver or bile ducts and intestines of mammals, where such animals are known as their “definite hosts.” The study aims to detect the genotype of Fasciola spp. from the livers of meat animals by using RFLP-PCR in samples collected from Fars province. Methods. Sixty Fasciola spp. samples were collected from infected slaughtered animals in three counties of Fars province, Iran (Jahrom, Nourabad Mamasani, and Kazeroun).Genomic DNA was extracted by the conventional phenol-chloroform method. For the study, PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1) region from Fasciola species were used to conduct the study. Results. The fragment of about 700 bp in all the Fasciola samples was amplified. In total, 43 samples of Fasciola gigantica and 17 samples of Fasciola hepatica were identified. Conclusion. The dominant Fasciola species in this region is Fasciola gigantica. Hence, it seems that hygienic policies should be developed to prevent and control fascioliasis because of the dominant species, Fasciola gigantica.

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Shаvkat Avezimbetov and Odilbek Dosumbetov. "Fasciolosis disease in cattle and sheep in Karakalpakstan in 2015-2020, prevalence, epizootology and treatment methods and results obtained." International Journal on Integrated Education 3, no. 12 (December 3, 2020): 83–86. http://dx.doi.org/10.31149/ijie.v3i12.924.

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Fastioliosis in the Republic of Karakalpakstan in acute, chronic and mixed course, acute course of F. gigantica-induced fasciolosis in sheep, clinical signs of fasciolosis, adult fasciolia, definitive host-infected fasciola larvae, possible factors, diagnosis, results of complete dissection of the liver in animals that died from the disease, drugs for the treatment of fasciolosis are described.

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Shаvkat Avezimbetov. "Fasciolosis disease in cattle and sheep in Karakalpakstan in 2015-2020, prevalence, epizootology and treatment methods and results obtained." International Journal on Integrated Education 3, no. 12 (December 8, 2020): 94–97. http://dx.doi.org/10.31149/ijie.v3i12.939.

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Fastioliosis in the Republic of Karakalpakstan in acute, chronic and mixed course, acute course of F. gigantica-induced fasciolosis in sheep, clinical signs of fasciolosis, adult fasciolia, definitive host-infected fasciola larvae, possible factors, diagnosis, results of complete dissection of the liver in animals that died from the disease, drugs for the treatment of fasciolosis are described.

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Kasahara, Shinpei, Yuma Ohari, Sakura Jin, Manuel Calvopina, Hidekazu Takagi, Hiromu Sugiyama, and Tadashi Itagaki. "Molecular characterization revealed Fasciola specimens in Ecuador are all Fasciola hepatica, none at all of Fasciola gigantica or parthenogenic Fasciola species." Parasitology International 80 (February 2021): 102215. http://dx.doi.org/10.1016/j.parint.2020.102215.

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Oktaviana, Putu Anna, Nyoman Adi Suratma, and I. Nengah Wandia. "Morphometry of Liver Fluke (Fasciola Gigantica) Infecting Balinese Cattle." Journal of Veterinary and Animal Sciences 2, no. 1 (January 31, 2019): 10. http://dx.doi.org/10.24843/jvas.2019.v02.i01.p02.

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Fasciola hepatica and Fasciola gigantica belong to Trematoda family which can cause fasciolosis. Fasciola hepatica is commonly found in temperate climates, while Fasciola gigantica is found in areas with wet tropical climates. Research on Fasciola gigantica especially in Bali is still very little, was just limited to the prevalence, epidemiology and histopathology of liver. This study aims to determine the morphometry of Fasciola gigantica infecting Bali cattle in Bali. This descriptive study used 43 samples of worms. Measurement of morphometry is done by microscope equipped with camera Axio Cam ERc 5S and software used is IMAGEJ and ZEN LITE. The morphometric analysis showed that there were two differentiating factors that is PB (Body Length) and LB (Body Width). The morphometric of Fasciola gigantica in Bali is smaller compared to Fasciola gigantica in general but larger than Fasciola hepatica.

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Zakharova, A. "Fasciola hepatica infestation." Veterinary Record 124, no. 1 (January 7, 1989): 23–24. http://dx.doi.org/10.1136/vr.124.1.23.

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Langridge, S. "Fasciola hepatica infestation." Veterinary Record 124, no. 17 (April 29, 1989): 471. http://dx.doi.org/10.1136/vr.124.17.471-c.

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Graham, E., and T. Harris. "Fasciola hepatica infestation." Veterinary Record 124, no. 4 (January 28, 1989): 103. http://dx.doi.org/10.1136/vr.124.4.103-c.

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Üsküdar, O., and E. Parlak. "Fasciola hepatica Infection." Video Journal and Encyclopedia of GI Endoscopy 1, no. 2 (October 2013): 482–83. http://dx.doi.org/10.1016/s2212-0971(13)70213-9.

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Bouix-Busson, D., D. Rondelaud, and C. Combes. "Fasciola hepatica L. :." Annales de Parasitologie Humaine et Comparée 60, no. 1 (1985): 5–10. http://dx.doi.org/10.1051/parasite/19856015.

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ICHIKAWA-SEKI, MADOKA, MAO PENG, KEI HAYASHI, TAKUYA SHORIKI, UDAY KUMAR MOHANTA, TOSHIYUKI SHIBAHARA, and TADASHI ITAGAKI. "Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China." Parasitology 144, no. 2 (November 2, 2016): 206–13. http://dx.doi.org/10.1017/s003118201600161x.

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SUMMARYThe well-known pathogens of fasciolosis, Fasciola hepatica (Fh) and Fasciola Gigantica (Fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. On the other hand, aspermic Fasciola flukes reported from Asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. The aim of this study was to reveal the origin of aspermic Fasciola flukes. The nuclear single copy markers, phosphoenolpyruvate carboxykinase and DNA polymerase delta, were employed for analysis of Fasciola species from China. The hybrid origin of aspermic Fasciola flukes was strongly suggested by the presence of the Fh/Fg type, which includes DNA fragments of both F. hepatica and F. gigantica. China can be regarded as the cradle of the interspecific hybridization because F. hepatica and F. gigantica were detected in the northern and southern parts of China, respectively, and hybrids flukes were distributed between the habitats of the two species. The Chinese origin was supported by the fact that a larger number of mitochondrial NADH dehydrogenase subunit 1 (nad1) haplotypes was detected in Chinese aspermic Fasciola populations than in aspermic populations from the neighbouring countries. Hereafter, ‘aspermic’ Fasciola flukes should be termed as ‘hybrid’ Fasciola flukes.

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Ollerenshaw, C. B., and E. G. Graham. "Differentiation of the rediae of Fasciola hepatica and Fasciola gigantica." Annals of Tropical Medicine & Parasitology 80, no. 5 (October 1986): 573–74. http://dx.doi.org/10.1080/00034983.1986.11812069.

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Abdollahi Khabisi, S., and B. Sarkari. "Detection of Fasciola Coproantigens Using Polyclonal Antibodies Produced Against Fasciola hepatica and Fasciola gigantica Secretory Excretory Antigens." Armaghane Danesh 27, no. 2 (March 1, 2022): 170–84. http://dx.doi.org/10.52547/armaghanj.27.2.170.

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Nukeri, Sophy, Mokgadi Pulane Malatji, Mita Eva Sengupta, Birgitte Jyding Vennervald, Anna-Sofie Stensgaard, Mamohale Chaisi, and Samson Mukaratirwa. "Potential Hybridization of Fasciola hepatica and F. gigantica in Africa—A Scoping Review." Pathogens 11, no. 11 (November 6, 2022): 1303. http://dx.doi.org/10.3390/pathogens11111303.

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The occurrence of Fasciola gigantica and F. hepatica in Africa is well documented; however, unlike in Asia, there is a paucity of information on the existence of hybrids or parthenogenetic species on the continent. Nonetheless, these hybrid species may have beneficial characteristics, such as increased host range and pathogenicity. This study provides evidence of the potential existence of Fasciola hybrids in Africa. A literature search of articles published between 1980 and 2022 was conducted in PubMed, Google Scholar, and Science Direct using a combination of search terms and Boolean operators. Fasciola species were documented in 26 African countries with F. hepatica being restricted to 12 countries, whilst F. gigantica occurred in 24 countries, identified based on morphological features of adult Fasciola specimens or eggs and molecular techniques. The co-occurrence of both species was reported in 11 countries. However, the occurrence of potential Fasciola hybrids was only confirmed in Egypt and Chad but is suspected in South Africa and Zimbabwe. These were identified based on liver fluke morphometrics, assessment of the sperms in the seminal vesicle, and molecular techniques. The occurrence of intermediate host snails Galba truncatula and Radix natalensis was reported in Ethiopia, Egypt, South Africa, Tanzania, and Uganda, where F. hepatica and F. gigantica co-occurrences were reported. The invasive Pseudosuccinea columella snails naturally infected with F. gigantica were documented in South Africa and Egypt. In Zimbabwe, P. columella was infected with a presumed parthenogenetic Fasciola. This suggests that the invasive species might also be contributing to the overlapping distributions of the two Fasciola species since it can transmit both species. Notwithstanding the limited studies in Africa, the potential existence of Fasciola hybrids in Africa is real and might mimic scenarios in Asia, where parthenogenetic Fasciola exist in most Asian countries. In South Africa, aspermic F. hepatica and Fasciola sp. have been reported already, and Fasciola hybrids have been reported? in Chad and Egypt. Thus, the authors recommend future surveys using molecular markers recommended to identify Fasciola spp. and their snail intermediate hosts to demarcate areas of overlapping distribution where Fasciola hybrids and/or parthenogenetic Fasciola may occur. Further studies should also be conducted to determine the presence and role of P. columella in the transmission of Fasciola spp. in these geographical overlaps to help prevent parasite spillbacks.

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Poovorawan, Kittiyod. "Fascioliasis." Thai Journal of Hepatology 2, no. 1 (January 4, 2019): 1–5. http://dx.doi.org/10.30856/th.jhep2019vol2iss1_01.

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Fascioliasis is infection by liver flukes, Fasciola spp. The two species that infect humans are Fasciola hepatica and Fasciola gigantica. Other herbivorous animals such as cattle, goats, water buffalo, horses, camels, hogs, rabbits, and deer can be infected with Fasciola spp. Humans acquire Fasciola after ingestion of food and water that are contaminated with Fasciola metacercaria, especially aquatic plants. Fascioliasis is usually mild disease in human. Clinical manifestrations are related with the the phases of infection. In migratory phase, symptoms may occur when metacercariae excyst in the duodenum and young liver flukes migrate through the intestinal wall to the liver capsule and hepatic parenchyma before reaching the bile ducts. Clinical symptoms include abdominal pain in the right hypochondrium, dyspepsia and anorexia, nausea, vomiting, and hepatomegaly. Systemic manifestration such as urticaria and eosinophilia are also common presentation. After Fasciola reside in the biliary tract, most of cases are asymptomatic. Biliary track obstruction can accidentally occur and might lead to cholangitis. Triclabendazole 10 mg/kg as a single dose is the current drug of choice for fascioliasis treatment and triclabendazole two doses of 10 mg/kg for 1 day is effective in severe or persistent infections, with cure rates of 79% and 92%, respectively. Currently, there is no vaccine for prevent Fasciola infection. Proper cooking or cleaning food especially aquatic plants before consumption will prevent accidental Fasciola infection. Adequate mass treatment among infected animals in endemic areas and proper sanitary in the livestock farm are important for disease control. Figure 1 ภาพถ่ายตัวเต็มวัยพยาธิ Fasciola hepatica (Linnaeus, 1758) (A) และ Fasciola gigantica (Cobbold, 1855) (B)(ได้รับการอนุเคราะห์ภาพจาก รศ.ดร.อุรุษา แทนขำ ภาควิชาปรสิตหนอนพยาธิ คณะเวชศาสตร์เขตร้อน มหาวิทยาลัยมหิดล)

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Caravedo, Maria A., Walter Ramirez, Maria L. Morales, Martha Lopez, Claire E. Janes, Brittany A. Bunag, Katie L. Mixon, A. Clinton White, Melinda B. Tanabe, and Miguel M. Cabada. "Fasciola hepatica Infection Risk for Adult Household Members Living with Children with Fascioliasis in Cusco, Peru." American Journal of Tropical Medicine and Hygiene 104, no. 6 (June 2, 2021): 2069–73. http://dx.doi.org/10.4269/ajtmh.21-0131.

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Abstract.Fasciola hepatica is highly prevalent in the highlands of Peru. School-age children have the greatest risk of infection. Mass treatment of at-risk populations has been proposed to control the infection and prevent complications. However, the decreasing effectiveness of triclabendazole raises concerns regarding this strategy. Previous studies reported aggregation of Fasciola infection among family members. This study aimed to determine the risk of fascioliasis among household members living with Fasciola-infected children identified through school-based testing. We conducted a cross-sectional study including adult members of households where children with and without fascioliasis were identified. Demographic, epidemiological, and socioeconomic information was collected. One blood sample was drawn to test for Fasciola antibodies, and three stool samples were collected for microscopy for Fasciola ova. We tested 326 adults from 213 households. Of these adult subjects, chronic fascioliasis (24 of 326, 7.4%) was the most common helminth infection. Thirty-nine subjects (12.7%) tested positive for Fasciola antibodies. Combining microscopy and serum antibody tests, 13.2% (43 of 326) had evidence of Fasciola infection. One third (104 of 326, 31.9%) of the participants lived with at least one child infected with Fasciola hepatica. Adults with fascioliasis were four times more likely to live with an infected child. Poverty and diet were associated with increased risk of Fasciola infection. Adults with fascioliasis were significantly more likely to live with Fasciola-infected children.

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ITAGAKI, T., M. ICHINOMIYA, K. FUKUDA, S. FUSYUKU, and C. CARMONA. "Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica." Parasitology 138, no. 10 (July 18, 2011): 1278–84. http://dx.doi.org/10.1017/s0031182011000965.

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SUMMARYExperiments on hybridization between Fasciola hepatica and Fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two Fasciola species. Molecular evidence for hybridization was based on the DNA sequence of the internal transcribed spacer 1 (ITS1) region in nuclear ribosomal DNA, which differs between the species. The results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. However, viable adults of the hybrids F1 and F2 were produced from both parental F. hepatica and F. gigantica. The hybrids inherited phenotypic characteristics such as ratio of body length and width and infectivity to rats from parental Fasciola hepatica and F. gigantica. These findings suggest that reproductive isolation is incomplete between Fasciola hepatica and F. gigantica. Adults of the hybrids F1 and F2 were completely different in mode of reproduction from aspermic Fasciola forms that occur in Asia and seem to be offspring originated from hybridization between F. hepatica and F. gigantica and to reproduce parthenogenetically.

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Peng, Mao, Mie Ichinomiya, Maiko Ohtori, Madoka Ichikawa, Toshiyuki Shibahara, and Tadashi Itagaki. "Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA." Parasitology Research 105, no. 3 (May 8, 2009): 809–15. http://dx.doi.org/10.1007/s00436-009-1459-0.

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Choi, Young-Jun, Santiago Fontenla, Peter U. Fischer, Thanh Hoa Le, Alicia Costábile, David Blair, Paul J. Brindley, Jose F. Tort, Miguel M. Cabada, and Makedonka Mitreva. "Adaptive Radiation of the Flukes of the Family Fasciolidae Inferred from Genome-Wide Comparisons of Key Species." Molecular Biology and Evolution 37, no. 1 (September 10, 2019): 84–99. http://dx.doi.org/10.1093/molbev/msz204.

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Abstract Liver and intestinal flukes of the family Fasciolidae cause zoonotic food–borne infections that impact both agriculture and human health throughout the world. Their evolutionary history and the genetic basis underlying their phenotypic and ecological diversity are not well understood. To close that knowledge gap, we compared the whole genomes of Fasciola hepatica, Fasciola gigantica, and Fasciolopsis buski and determined that the split between Fasciolopsis and Fasciola took place ∼90 Ma in the late Cretaceous period, and that between 65 and 50 Ma an intermediate host switch and a shift from intestinal to hepatic habitats occurred in the Fasciola lineage. The rapid climatic and ecological changes occurring during this period may have contributed to the adaptive radiation of these flukes. Expansion of cathepsins, fatty-acid-binding proteins, protein disulfide-isomerases, and molecular chaperones in the genus Fasciola highlights the significance of excretory–secretory proteins in these liver-dwelling flukes. Fasciola hepatica and Fasciola gigantica diverged ∼5 Ma near the Miocene–Pliocene boundary that coincides with reduced faunal exchange between Africa and Eurasia. Severe decrease in the effective population size ∼10 ka in Fasciola is consistent with a founder effect associated with its recent global spread through ruminant domestication. G-protein-coupled receptors may have key roles in adaptation of physiology and behavior to new ecological niches. This study has provided novel insights about the genome evolution of these important pathogens, has generated genomic resources to enable development of improved interventions and diagnosis, and has laid a solid foundation for genomic epidemiology to trace drug resistance and to aid surveillance.

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Davelois Atac, Kelly Roxana, Cesar Augusto Jara Campos, Andrea Victoria Coronado Figarella, Omar Julio Escalante Gavidia, and Hermes Mario Escalante Añorga. "Rendimiento diagnóstico de la prueba rápida fasciorap basada en la técnica de inmunocromatografía o flujo lateral para la detección de anticuerpos en pacientes con Fasciolosis humana." Revista de Gastroenterología del Perú 42, no. 2 (June 30, 2022): 92. http://dx.doi.org/10.47892/rgp.2022.422.1374.

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Objetivo: Determinar el rendimiento diagnóstico de la técnica de inmnunocromatografía o flujo lateral para la detección de anticuerpos en pacientes con Fasciolosis humana. Materiales y métodos: Estudio observacional, prospectivo y de corte transversal. Hemos desarrollado una prueba de flujo lateral (Fasciorap) para el diagnóstico serológico de las Fasciolosis humana por Fasciola hepatica, compuesta por antígenos de excreción-secreción de formas adultas conjugadas con orocoloidal de 40 nm y una proteína A e IgG de conejo anti Fasciola hepatica como reactivos detectores en la línea de prueba y control, flanqueados por almohadillas en un cassette. Se evaluaron 240 sueros, 120 positivos, 50 sueros de pacientes con otras parasitosis, 20 de pacientes con enfermedades infecciosas y 50 sueros de personas no parasitadas, la interpretación de resultados se realizó por inspección visual a los 15 minutos de aplicada las muestras. Resultados: La prueba detectó la presencia de anticuerpos en el suero de pacientes con fasciolosis, alcanzando una sensibilidad de 92,5%, una especificidad de 94,17%, un valor predictivo positivo de 94,07% y negativo de 92,62%; con 100% de concordancia en la repetibilidad y reproducibilidad. Conclusiones: Fasciorap detecta casos de fasciolosis, por lo tanto, es una potencial prueba diagnóstica en zonas endémicas donde se requiere pruebas de punto de atención

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Bourée, P. "Diffusion de Fasciola hepatica." Médecine et Santé Tropicales 27, no. 2 (May 2017): 145. http://dx.doi.org/10.1684/mst.2017.0670.

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Aksoy, D. Y., U. Kerimoglu, A. Oto, S. Erguven, S. Arslan, S. Unal, F. Batman, and Y. Bayraktar. "Infection with Fasciola hepatica." Clinical Microbiology and Infection 11, no. 11 (November 2005): 859–61. http://dx.doi.org/10.1111/j.1469-0691.2005.01254.x.

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Santarém, Vamilton Alvares, Raimundo Alberto Tostes, Haroldo Alberti, and Osimar de Carvalho Sanches. "Fasciola hepatica in capybara." Acta Tropica 98, no. 3 (July 2006): 311–13. http://dx.doi.org/10.1016/j.actatropica.2006.05.004.

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Lee, Chung G., and Gary L. Zimmerman. "Banding Patterns of Fasciola hepatica and Fasciola gigantica (Trematoda) by Isoelectric Focusing." Journal of Parasitology 79, no. 1 (February 1993): 120. http://dx.doi.org/10.2307/3283290.

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Mahardika, Mesia Margi, Sri Agus Sudjarwo, and Setiawan Koesdarto. "Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro." KnE Life Sciences 3, no. 6 (December 3, 2017): 266. http://dx.doi.org/10.18502/kls.v3i6.1135.

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The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC50 and LC90. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC50 and LC90. The results were LC50 of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC90 was 8.4% at 10 hours. Key words: Ocimum sanctum Linn. leaves, Fasciola gigantica, ethanol extract, in vitro.

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Itagaki, Tadashi, and Ken-ichi Tsutsumi. "Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepaticaand Fasciola giganticadetermined by ITS-2 sequence of nuclear rDNA." International Journal for Parasitology 28, no. 5 (May 1998): 777–81. http://dx.doi.org/10.1016/s0020-7519(98)00037-x.

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Saijuntha, Weerachai, Chairat Tantrawatpan, Takeshi Agatsuma, Chennan Wang, Pewpan M. Intapan, Wanchai Maleewong, and Trevor N. Petney. "Revealing genetic hybridization and DNA recombination of Fasciola hepatica and Fasciola gigantica in nuclear introns of the hybrid Fasciola flukes." Molecular and Biochemical Parasitology 223 (July 2018): 31–36. http://dx.doi.org/10.1016/j.molbiopara.2018.06.004.

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Postevoy, Andreyanov, and Puzanova. "STUDY OF MOLLUSCS’ INFESTATION WITH FASCIOLA HEPATICA IN BRYANSK REGION." THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL, no. 20 (May 14, 2019): 481–86. http://dx.doi.org/10.31016/978-5-9902340-8-6.2019.20.481-486.

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The intermediate host of Fasciola hepatica is Lymnaea truncatula mollusk. However, participation of other species of mollusks (L. stagnalis, Galba palustris, etc.) in the development cycle of F. hepatica is not excluded. In this connection, we have studied the distribution of mollusks of these species in the territory of the Bryansk region. The aim of the work was to carry out a study on the invasion of fasciol larvae of mollusks on pastures of trematodiasis-unfriendly farms of the Bryansk region. On farms, the density of mollusks and their species, as well as the dynamics of the infection of mollusks by the partenite stages of F. hepatica, were determined. The work was carried out from May to October 2018 on 3 pastures with an area of 2 to 5 hectares. The study was subjected to mollusks: small pond snail L. truncatula in the amount of from 149 to 151 specimens, ordinary pond snail – L. stagnalis from 10 to 18 specimens, marsh pond snail – L. palustris from 30 to 85 specimens, iushkoviy pond snail – L. auricularia from 4 to 21 specimens. Mollusks were examined every month during the pasture period (May-October) for infection with fasciol larvae by the compressor method. It was revealed that only small pond snail L. truncatula was infested by the larval stages of development of fasciol. The rest of the limneids were free from this trematodase invasion. The small pond snail was infected more intensely in September. The fasciol larvae of the small pond snail were recorded in May, June, August and September; the infection rate was 1.3%, 2.6%, 6.6% and 8%, respectively.

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Bich Nga, Nguyen Thi, Do Thi Roan, Nguyen Thi Khue, Huynh Hong Quang, Nguyen Van De, and Le Thanh Hoa. "Analysis of genetic distance and phylogenetic relationships of “natural/admixed” and “introgressive” hybridization of Fasciola spp. in Vietnam." Vietnam Journal of Biotechnology 16, no. 3 (December 18, 2018): 423–30. http://dx.doi.org/10.15625/1811-4989/16/3/13467.

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Fasciola hepatica, F. gigantica and “intermediate” forms of Fasciola species, are responsible for fascioliasis in ruminant animals and humans in many countries. There are two forms: “natural/admixed” and “introgressive” hybridization. Vietnam is a "hot spot" country, where the “hybrid” Fasciola forms have been found nationwide. Determination of phylogenetic relationships and genetic distance of these hybrid forms with those in family Fasciolidae and class Trematoda (Phyllum Platyhelminthes) is necessary to confirm their taxonomic classification. In this study, we sequenced the ITS1 and ITS2 for discrimination of two kinds of hybrid Fasciola mentioned above. The deduced amino acid sequences of the entire mitochondrial genes, cytochrome b (cob), nicotinamide dehydrogenase 1 (nad1) and cytochrome oxidase 1 (cox1), from two hybrid Fasciola spp. including Fsp-DL11-VN (from buffalo, admixed hybrid), Fsp-FH1-VN (from human, introgressive hybrid) and “pure” F. gigantica (Fgig-T4V-VN, from cattle) were obtained and combined (cob+nad1+cox1). These sequences were used as markers for phylogenetic analysis together with 28 representative isolates/species of Fasciolidae and Trematoda. Genetic distances between 13 isolates/species in the family Fasciolidae have also been determined to accurately account for their species-relationships. As results, pairwise genetic distance calculation showed that the distance rate was only 0.4% – 0.7% between Fasciola spp. hybrids of Vietnam and China, slightly higher, 1.3 – 2.0% with “pure” F. gigantica, whilist this rate was quite high compared with F. hepatica (5.7% – 5.9%), with Fasciolopsis buski (20.6% – 21.0%), and two other fasciolids, Fasciola jacksoni and Fascioloides magna (11.0% – 12.6%). The phylogenetic tree of 31 strains/species showed 5 distinct groups, corresponding to 5 families, ie., Fasciolidae, Echinostomatidae, Echinochasmidae, Heterophyidae, Opisthorchiidae and an outgroup, Schistosomatidae. Two "hybrid" Fasciola species of Vietnam (Fsp-FH1-VN and Fsp-DL11-VN) grouped with the reference "hybrid" Fsp-GHL-CN strain of China; and “pure” Fgig-T4V-VN with the two “pure” F. gigantica, Fgig-Bali-ID (Indonesia) and Fgig-GX-CN (China). These results confirmed that “hybrid” Fasciola spp. were of the maternally inherited mitochondrial lineage from F. gigantica.

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Postevoy. "STUDY OF MOLLUSCS IN BIOTOPES ON PASTURES CONTAMINATED BY FASCIOLA HEPATICA." THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL, no. 23 (April 18, 2022): 371–76. http://dx.doi.org/10.31016/978-5-6046256-9-9.2022.23.371-376.

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The mollusk Lymnaea truncatula is an intermediate host of Fasciola hepatica. However, the involvement of other mollusk species (L. stagnalis, Galba palustris, etc.) in the F. hepatica development cycle might take place. In this connection, we have studied the spread of molluscs of these species in areas contaminated by Fasciola hepatica. The research purpose was to study the infection of molluscs by Fasciola larvae in the habitats (biotopes) on pastures contaminated by trematodiasis in the Bryansk Region. We determined the population density of molluscs and their species on the pastures, and the infection dynamics of parthenita stages of F. hepatica in molluscs. The study was performed on 3 pastures of 12 to 27 hectares from April to October 2021. The following molluscs were examined: 10 to 93 specimens of the snail L. truncatula, 11 to 24 specimens of the great pond snail L. stagnalis, and 12 to 42 specimens of the aquatic snail L. palustris. Molluscs were examined for infection with Fasciola larvae by the compression method every month during the grazing period (April-October). As a result of the study, the snail L. truncatula was only found to be infected by larval stages of Fasciola. The remaining lymnaeids were free from this trematode invasion. The snail L. truncatula was more infected with Fasciola in September. The Fasciola larvae in the snail were observed in May, August and September with the infection rate of 2.3%, 3.5% and 4.3%, respectively.

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Shinggu, P. A., O. T. Olufemi, J. A. Nwuku, E. B. T. Baba-Onoja, and P. D. Iyawa. "Liver Flukes Egg Infection and Associated Risk Factors in White Fulani Cattle Slaughtered in Wukari, Southern Taraba State, Nigeria." Advances in Preventive Medicine 2019 (March 19, 2019): 1–5. http://dx.doi.org/10.1155/2019/2671620.

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Fasciola, Fascioloides, and Dicrocoelium cause liver fluke diseases in ruminants and are of zoonotic and economic importance. This cross-sectional study aimed to determine the prevalence of liver fluke egg infection in White Fulani Cattle slaughtered in Wukari Cattle market abattoir in Wukari, Taraba State. A total of 262 gallbladders were collected and their contents were analyzed for the presence of eggs of liver flukes using sedimentation technique. Descriptive analysis was done using SPSS version 20 for window and Pearson’s Chi-Square (χ2) was used to evaluate the association between variables. Fasciola and Dicrocoelium eggs were encountered. Only 74 (28.2%) samples were positive for Fasciola egg and 211 (80.5%) for Dicrocoelium. The prevalence of liver fluke infection revealed 25% and 28.6% Fasciola eggs for male and female animals, respectively, while lancet fluke had 83.3% for male and 80.3% for female. Fasciola eggs were recovered in 20.8% of animals aged less 3 years (<3yrs) and 29.9% was recorded in animals 3 years and above (≥ 3yrs) while 81.3% for animals ≥ 3yrs and 77.1% for animals <3yrs were recorded for Dicrocoelium eggs. The body condition score-based prevalence for Fasciola yielded 38.1%, 26.8%, and 14% for poor, average, and good, respectively, while Dicrocoelium yielded 85.7%, 79.3%, and 85.7%. There was a significant difference between the body condition scores for fasciolosis. Only 59% harboured single infection with eggs of Dicrocoelium (P< 0.05) while 6.9% harboured Fasciola eggs. Mixed infection associating Fasciola and Dicrocoelium was observed in 21.4% of the sample cattle. Liver fluke infections: fasciolosis and dicrocoeliosis occur among White Fulani cattle in Wukari and these infections are associated with the body condition score of the animals. This greatly affects the cattle production. There is a need to institute adequate control programmes complemented with good well-planned management practices in any production system involving cattle in Wukari.

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Chartier, C., M. Bushu, and U. Anican. "Les dominantes du parasitisme helminthique chez les bovins en Ituri (Haut-Zaïre). II. Les associations parasitaires." Revue d’élevage et de médecine vétérinaire des pays tropicaux 43, no. 4 (April 1, 1990): 491–97. http://dx.doi.org/10.19182/remvt.8772.

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Sept cent quatre-vingt un bovins ont été examinés à l'abattoir de Bunia (Ituri), d'août 1986 à décembre 1987, pour étudier les deux associations parasitaires suivantes : Fasciola sp., Schistosoma sp., paramphistomes et Fasciola sp., Haemonchus sp., Oesophagostomum sp. Les prévalences pour chaque parasite sont élevées : 96,5 p. 100 pour les paramphistomes, 58,1 p. 100 pour Schistosoma sp., 58,7 p. 100 à 61,9 p. 100 pour Fasciola sp., 90,5 p. 100 pour Haemonchus sp. et 75,5 p. 100 pour Oesophagostomum sp. Concernant l'association à trématodes, 41,3 p. 100 des 516 bovins examinés hébergent les trois parasites simultanément et une liaison significa t ive positive existe entre l'infection à Fasciola sp. et Schistosoma sp. Concernant l'association à Fasciola sp., Haemonchus sp. et Oesophago s tomum sp., 44,5 p. 100 des 265 bovins examinés présentent les trois helminthe s en même temps mais les infestations semblent indépendantes. Pour cette dernière association, les lésions organiques correspondantes sont modérées, laissant supposer des charges parasitaires peu importantes.

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Fahlevi, M. R., I. S. Pratama, and M. Sriasih. "Anthelmintic activity assay of Starchytarpeta jamaicensis L. Vhal tea against Fasciola sp." IOP Conference Series: Earth and Environmental Science 913, no. 1 (November 1, 2021): 012096. http://dx.doi.org/10.1088/1755-1315/913/1/012096.

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Abstract Stachytarpeta jamaicensis L. Vhal (SJ) tea has been empirically used as an anthelmintic, but scientific evidence on its use as an anthelmintic against Fasciola sp. is still limited. The aim of this study was to determine the effective concentration of SJ tea as an anti-Fasciola. S. jamaicensis leaves were cut, dried and then packed in the form of tea bags. The presence of secondary metabolites in the tea was also analyzed phytochemically. The anthelmintic activity assay of SJ tea was carried out using an in vitro experimental design with a post-test with control group design. The in vitro test consisted of negative control (0.9% NaCl), positive control (10% Albendazole) and SJ tea with a concentration of 10%, 5%, and 2.5%. The worm’s movement was observed and the time required for the death of Fasciola was recorded and analyzed. Phytochemical analysis shows that SJ tea contained alkaloid compounds, flavonoids, saponins, tannins and triterpenoids. Time required for death of Fasciola in the positive control, the negative control, the tea concentration of 10%, 5%, and 2.5% were 18.75, 168.75, 23.75, 42.5, and 66.25 min, respectively. S. jamaicensis tea with a concentration of 10% showed comparable effectiveness (p>0.05) to standard reference 10% Albendazole as an anti-Fasciola and resulted in the fastest death of Fasciola fluke compared to other concentrations of the tea. The results of this study indicate that SJ tea can be used as an alternative in overcoming Fasciola infestation. Further works are required to determine its safety when used in vivo.

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Luo, Xier, Kuiqing Cui, Zhiqiang Wang, Zhipeng Li, Zhengjiao Wu, Weiyi Huang, Xing-Quan Zhu, Jue Ruan, Weiyu Zhang, and Qingyou Liu. "High-quality reference genome of Fasciola gigantica: Insights into the genomic signatures of transposon-mediated evolution and specific parasitic adaption in tropical regions." PLOS Neglected Tropical Diseases 15, no. 10 (October 5, 2021): e0009750. http://dx.doi.org/10.1371/journal.pntd.0009750.

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Fasciola gigantica and Fasciola hepatica are causative pathogens of fascioliasis, with the widest latitudinal, longitudinal, and altitudinal distribution; however, among parasites, they have the largest sequenced genomes, hindering genomic research. In the present study, we used various sequencing and assembly technologies to generate a new high-quality Fasciola gigantica reference genome. We improved the integration of gene structure prediction, and identified two independent transposable element expansion events contributing to (1) the speciation between Fasciola and Fasciolopsis during the Cretaceous-Paleogene boundary mass extinction, and (2) the habitat switch to the liver during the Paleocene-Eocene Thermal Maximum, accompanied by gene length increment. Long interspersed element (LINE) duplication contributed to the second transposon-mediated alteration, showing an obvious trend of insertion into gene regions, regardless of strong purifying effect. Gene ontology analysis of genes with long LINE insertions identified membrane-associated and vesicle secretion process proteins, further implicating the functional alteration of the gene network. We identified 852 predicted excretory/secretory proteins and 3300 protein-protein interactions between Fasciola gigantica and its host. Among them, copper/zinc superoxide dismutase genes, with specific gene copy number variations, might play a central role in the phase I detoxification process. Analysis of 559 single-copy orthologs suggested that Fasciola gigantica and Fasciola hepatica diverged at 11.8 Ma near the Middle and Late Miocene Epoch boundary. We identified 98 rapidly evolving gene families, including actin and aquaporin, which might explain the large body size and the parasitic adaptive character resulting in these liver flukes becoming epidemic in tropical and subtropical regions.

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Hourdin, P., D. Rondelaud, and J. Cabaret. "Effect of concurrent infection with Muellerius capillaris on the development of redial generations of Fasciola hepatica in Lymnaea truncatula." Journal of Helminthology 66, no. 2 (June 1992): 108–17. http://dx.doi.org/10.1017/s0022149x00012670.

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ABSTRACTThe rediae of Fasciola hepatica were counted according to generation in adult and juvenile Lymnaea truncatula following single infection with Fasciola hepatica, double infection with F. hepatica and then Muellerius capillaris, or double infection with M. capillaris and F. hepatica. The rediae found in double infections were essentially first generation and an early cohort from the second generation. The following differences were observed in adult snails which underwent double infection when compared to corresponding single infections: i) dependent rediae were almost completely absent; ii) degenerating independent rediae were found in identical or decreased numbers; iii) living independent rediae of the first generation were decreased in number, while those of the second generation had variable decreased numbers. The results were similar in juvenile snails with double infections, except that the numbers of degenerating independent rediae were higher than those found in corresponding single infections and the numbers of rediae of the second generation were increased. The order of exposure in double infections had no influence on the number and maturation of fasciolid rediae.

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Evack, Jennifer Giovanoli, Remo S. Schmidt, Sylwia D. Boltryk, Till S. Voss, Annour A. Batil, Bongo N. Ngandolo, Helena Greter, Jürg Utzinger, Jakob Zinsstag, and Oliver Balmer. "Molecular Confirmation of a Fasciola Gigantica × Fasciola Hepatica Hybrid in a Chadian Bovine." Journal of Parasitology 106, no. 2 (April 24, 2020): 316. http://dx.doi.org/10.1645/19-66.

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Tran, Diem Hong, and Huong Thi Thu Phung. "Detecting Fasciola hepatica and Fasciola gigantica microRNAs with loop-mediated isothermal amplification (LAMP)." Journal of Parasitic Diseases 44, no. 2 (September 28, 2019): 364–73. http://dx.doi.org/10.1007/s12639-019-01164-w.

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Marcilla, A., M. D. Bargues, and S. Mas-Coma. "A PCR-RFLP assay for the distinction between Fasciola hepatica and Fasciola gigantica." Molecular and Cellular Probes 16, no. 5 (October 2002): 327–33. http://dx.doi.org/10.1006/mcpr.2002.0429.

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Salihu, M. D., A. I. Musawa, B. Garba, Y. Yakubu, M. B. Bello, A. A. Magaji, A. U. Junaidu, A. H. Jibril, F. M. Ballah, and C. R. Achi. "Molecular characterization and species differentiation of Fasciola parasite isolated from cattle slaughtered at Sokoto modern abattoir, Nigeria." Nigerian Journal of Parasitology 43, no. 1 (May 28, 2022): 102–8. http://dx.doi.org/10.4314/njpar.v43i1.14.

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Fascioliasis is an important zoonotic disease endemic in many African countries, causing significant financial losses due to reduced productivity and visceral condemnation at slaughter. Fasciola hepatica and F. gigantica are the main causative agents of fascioliasis in domestic animals and humans. Traditional species differentiation based on their morphometric characteristics is subjective and can be challenging. This study was undertaken to identify the Fasciola species associated with cattle infection using a molecular approach. Thirty-eight Fasciola parasite samples collected from cattle slaughtered at the Sokoto modern abattoir were characterised by PCR-RFLP analysis of ITS1 and ITS2 genes using RsaI restriction enzyme, sequencing, and phylogenetic analysis. The results revealed that the isolates belonged to the F. gigantica species based on RFLP patterns. Similarly, phylogenetic results showed clustering with F. gigantica when compared with sequences from neighbouring African countries obtained from the GenBank. This study affirmed that F. gigantica is the predominant Fasciola species affecting cattle in Sokoto state, Nigeria. The results also demonstrate the discriminatory potentials of RFLP and its ability to determine genetic variability among Fasciola Parasites.

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Asmaydo, Dhio, Iwan Sahrial Hamid, Muchammad Yunus, Kusnoto Kusnoto, Mohammad Sukmanadi, and Endang Suprihati. "The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro." Journal of Parasite Science 3, no. 1 (December 4, 2019): 15. http://dx.doi.org/10.20473/jops.v3i1.16426.

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The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

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Journal articles on the topic 'Fasciola'

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