Dissertations / Theses on the topic 'Farm manure in methane production'

[Truncated abstract] In Australia, there is pressure to reduce the amount of methane produced by ruminant livestock because they are the single largest source of methane emitted from anthropogenic sources, accounting for 70.7% of agricultural methane emissions. In addition, methane production represents a loss of gross energy intake to the animal. The organisms that are responsible for methane production in the animal gut are a distinct group of Archaea called methanogens. Methanogens occupy three different niches within the rumen. Some live freely in the rumen digesta (planktonic), others are attached to the outer surface of the rumen ciliates (ectosymbiotic), and some reside within the ciliates (endosymbiotic). The types and number of methanogens, as well as rumen ciliates and their symbiotic interactions, influence the amount of methane produced from the rumen. These factors in turn are affected by many factors, including diet and ruminal retention time. In this thesis, I tested the general hypothesis that increasing the amount of grain in the diet and reducing the retention time would affect the abundance and diversity of methanogens in their different niches, including their association with ruminal ciliates. Twenty-four fistulated sheep were used in a complete factorial design with the sheep randomly divided into four groups. ... The change in DGGE banding patterns and Shannon indices when sheep were fed grain indicated that the types of methanogens changed when sheep were fed low and high grain diets, but their diversity did not. In contrast, the diversity of rumen ciliates decreased when sheep were fed a high grain diet. A total of 18 bands from the DGGE analysis of the ciliates were sequenced. All except one, which was 98% similar to Cycloposthium sp. not found previously in the rumen, matched the sequences for previously identified rumen ciliates. Some of the rumen ciliates identified were not present in sheep fed the high grain diet. On a high grain diet, methanogens associate endosymbiotically with rumen ciliates to get better access to hydrogen. It appears that the association between methanogens and rumen ciliates is dictated by the availability of hydrogen in the rumen and not the generic composition of the ciliate population. Furthermore, endosymbiotic methanogens appear to produce less methane than methanogens in other niches. The pot scrubbers did not change ruminal retention time but they did reduce the acetate/propionate measurements observed in sheep on the high grain treatment. The reason why pot scrubbers had this effect remains unknown, but it is interesting to consider that some physical interaction has occurred between the pot scrubbers, the grain and the sheep that has improved the fermentation parameters in sheep fed a high grain diet. The results from this study have advanced our understanding of the interaction between methanogens and ruminal ciliates, and methanogenesis in the rumen in response to dietary changes and mechanical challenges. Extending this work to look more specifically at the species of methanogens that are most closely linked to high methane production and how they interact with the ruminal ciliates will be critical for manipulating enteric greenhouse gas emissions.

Biogas is an expanding sector within the broad field of agriculture and animal production. Small-scale biogas offers local combined power and heating production and the substrate is transformed into high-quality biological fertilizer. This bachelor thesis focuses on a pig farm in south-western Sweden, where biogas is produced from pig manure, evaluates and suggests ways of optimizing the process and investigates whether investing in an upgrading plant would be a feasible and more cost-efficient option. The results show that the biogas plant is working well, although the production differs from the original plans. This shows in turn that planning and examining the basic conditions before making the investment is of great importance, as well as monitoring and keeping detailed statistics of the running process. Logistical factors make optimizing the process through additional substrates difficult. The thesis shows that investing in a Biosling upgrading plant would be a profitable option, supposing that the upgraded gas is sold via the natural gas infrastructure. Furthermore, many farmers are interested in producing their own fuel for tractors and other machines, which offers more future alternatives for the upgraded biogas. However, biogas producers in Sweden today are not offered any particular subsidies, which makes it especially hard for small-scale producers.

This research investigates an alternative approach to the use of two wastes from agricultural and livestock activities developed in Colombia. Swinemanure and coffee mucilage were used to evaluatean anaerobic co-digestion process focused on hydrogen production. In addition, the aims covered a further stage in order to close the cycle of the both wastes. The thesis was conducted in three phases : 1. Evaluation of hydrogen production from the co-digestion of coffee mucilage and swine manure during dark fermentation ; 2. Trends over retention time through the monitoring of microorganisms by quantitative PCR and other parameters incluiding pH, oxidation reduction potential, and hydrogen partial pressure ; 3. Treatment of the effluent from hydrogen production process by anaerobic digestion with methane production. The experimental results showed that mixtures of both wastes are able to produce hydrogen. A substrate ratio of 5:5, which was associated with a C/N ratio of 53, was suitable for hydrogen production. Moreover, the stability and optimization of the process were evaluated by increasing the influent organic load rate. This wasthe best experimental condition in terms of average cumulative hydrogen volume, production rate and yield which were 2661 NmL, 760 NmLH2/Lwd and 43 NmL H2/gCOD, respectively. This performance was preserved over time, which was verified through the repetitive batch cultivation during 43 days. Two trends were identified over retention time associated with similar cumulative hydrogen, but with differences in lag-phase time and hydrogen production rate. T.thermosaccharolyticum was the dominating genus during the short trend related to the shortest lag phase time and highest hydrogen production rate. The long trends were associated with a decrease of Bacillus sp. concentration at the beginning of the experiments and with the possible competition for soluble substrates between T.thermosaccharolyticum and Clostridium sp. The third phase showed that the use of a second stage to produce methane was useful enhancing the treatment of both wastes. Finally, the overall energy produced for both biofuels (Hydrogen andmethane) showed similar levels with other process. However, hydrogen was around the 10% of the overall energy produced in the process. In addition, both gases could be mixed to produce biohythane which improves the properties of biogas.

Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第17619号
農博第1981号
新制||農||1009(附属図書館)
学位論文||H25||N4740(農学部図書室)
30385
京都大学大学院農学研究科農学専攻
(主査)教授 稲村 達也, 教授 白岩 立彦, 教授 廣岡 博之
学位規則第4条第1項該当

This thesis links papers reporting field measurements, modelling studies and reviews of greenhouse gas (GHG) emissions and their abatement from agriculture, in particular from livestock production. The aims of the work were to: quantify GHG emissions from litter-based farmyard manures; evaluate means by which GHG emissions from agricultural production may be abated; assess synergies and conflicts between the abatement of other N pollutants on emissions of nitrous oxide (N2O); analyse two records of soil temperature from 1976-2010 from Wolverhampton (UK) and Vienna (Austria). Agricultural emissions of GHGs are not readily abated by ‘end of pipe’ technologies. Large decreases in agricultural GHG emissions may require changes in the production and consumption of food that could have unwelcome impacts on both consumers and producers. However, identifying and prioritizing both modes and locations of production, together with utilizing inputs, such as N fertilizer and livestock feeds, more efficiently can reduce GHG emissions while maintaining outputs. For example, GHG emissions from livestock production may be lessened by increasing the longevity of dairy cows, thereby decreasing the proportion of unproductive replacement animals in the dairy herd. Sourcing a larger proportion of calves from the dairy herd would decrease emissions of GHGs from beef production. The distance between the region of food production to that of consumption has relatively little impact on total GHG emissions per tonne of food product. Due to greater productivity or lesser energy inputs, importing some foods produced in other parts of the world may decrease GHG emissions per tonne compared with UK production, despite the additional emissions arising from long-distance transport. Manure application techniques to abate ammonia (NH3) emissions do not axiomatically increase emissions of N2O and may decrease them. Soil temperature measurements from 1976 to 2010 were consistent with the warming trends reported over the last 40 years.

Biomass and biomass-derived waste are important renewable energy sources which plays a vital role in greenhouse gas reduction from fossil fuel.  Biomass can be degraded in a process known as anaerobic digestion (AD) to produce biogas. Biogas is a mixture of methane and carbon dioxide which is utilized as a renewable source of energy. This project was based on the investigation of AD process in Nordvästra Skånes Renhållnings AB (NSR) a biogas facility in Helsingborg Sweden. A lab simulation of NSR digesters was conducted to evaluate the effects of swine manure on AD using two continuously stirred tank reactors (CSTR) R1 (control) and R2 with a working volume of 4L for 21 weeks. The study was divided into 4 periods and the investigation was carried out by increasing the organic loading rate (OLR) step wise from 2.5 to 3.6 gVSL-1day-1. To assess the effects of swine manure, the performance and stability of the reactors were monitored by collecting data from process parameters. These process parameters included biogas production, pH, volatile fatty acids, methane yield, methane content and organic solids (total and volatile solids). Increase in OLR resulted in increase biogas production in both reactors, however R2 with additional swine manure (15%) produced more biogas than R1. Methane yield was fairly stable during the experiment and had a similar trend in both reactors, but however R2 had a slightly higher average yield (730±60 mLCH4 gVS-1) than R1 (690±60 mLCH4 gVS-1) during the entire experiment. Increase OLR resulted in increase VFA in period 2; R2 with additional swine manure had a lower peak VFA concentration of 25 mM as compared to 33mM in R1.  The characteristics of NSR substrate mix and swine manure provided a good buffering system (stable pH), and reactors were still running stably at 3.6 gVSL-1day-1. Furthermore swine manure was investigated to contain macro-nutrients and trace metals which might have enhanced the AD process in R2 containing more Co, Zn, Ni and Mo than R1. Since this investigation was a simulation, the waste mix used at NSR contained 7% swine manure, this made it difficult to give clearer conclusions about the effects of co-digestion of swine manure on the biogas process since the control (R1) had 7% swine manure. Keywords: Anaerobic digestion, co-digestion, swine manure, substrate mix, organic loading rate, biogas production, methane yield, VFA, process parameters, CSTR.

Anaerobic digestion is the process by which organic carbon is converted into biogas in the form of carbon dioxide (CO2) and methane (CH4). Both of these products are greenhouse gases that contribute to global warming. Therefore if anaerobic reactors are improperly maintained and biogas is leaked or intentionally released into the atmosphere because biogas production exceeds household demand, these reactors may become generators of greenhouse gas emissions instead of sustainable energy producers. The objective of this research was to develop a framework to assess if the demand for biogas by a rural adopter of an anaerobic digester matched with the associated local gas production. A literature review of the energy required to prepare commonly consumed food of rice and beans was conducted to establish required household biogas volumes. This review determined that 0.06 m3 of methane was required to prepare a half a kg of rice (on a dry weight basis) and 0.06 m3 of methane was required to prepare a half a kg of beans (on a dry weight basis). Furthermore an analysis of occupants of a rural Panamanian town was performed along with a design model for rural anaerobic reactor gas production to determine if an overproduction of biogas would occur if anaerobic reactors were built for families who owned swine. It was determined using this approach that all of the fifteen household would experience an overproduction of biogas based on household demand of methane and therefore would risk the release of greenhouse gases. Household size ranged from one to seven occupants and swine ownership ranged from one to fifteen per household. The differences of biogas supply with respect to demand from these fifteen situations ranged from 0.09 to 0.35 m3 of a biogas with 40% methane and 0.27 to 6.17 m3 of excess biogas with a methane content of 70% per household per day. An average of 0.45 m3 of a biogas with 40% methane per household per day was calculated and 0.87m3 for 70% methane for all fifteen households, excluding one outlier. However, because this research uses a model based on plug flow reactor mechanics, results may produce varied results from other studies concerning small scale anaerobic digestion.

Mathematical modeling in animal agriculture can be applied at various levels including at the tissue, organ, animal, farm, regional and global levels. The purposes of this research were i) to evaluate models used to estimate volatile fatty acid (VFA) and methane (CH4) production and assess their impact on regional enteric CH4 inventory, and ii) to develop a process-based, whole-farm model to estimate net farm GHG emissions. In the first study, four VFA stoichiometric models were evaluated for their prediction accuracy of rumen VFA and enteric CH4 production. Comparison of measured and model predicted values demonstrated that predictive capacity of the VFA models varied with respect to the type of VFA in rumen fluid which impacted estimated enteric CH4 production. Moving to a larger scale assessment, we examined the enteric CH4 inventory from Manitoba beef cattle (from 1990 to 2008) using two mechanistic rumen models that incorporate VFA stoichiometric models: COWPOLL and MOLLY, and two empirical models: Intergovernmental Panel on Climate Change (IPCC) Tier 2 and a nonlinear equation (Ellis). The estimated absolute enteric CH4 production varied among models (7 to 63%) indicating that estimates of GHG inventory depend on model selection. This is an important consideration if the values are to be used for management and/or policy-related decisions. Development of models at the individual farm component level (animal, soil, crop) does not accurately reflect net GHG emissions generated from the whole production system. We developed a process-based, whole-farm model (Integrated Components Model, ICM), using the existing farm component models COWPOLL, manure-DNDC and some aspects of IPCC to integrate farm components and their associated GHG emissions. Estimates of total farm GHG emissions and their relative contribution using the ICM were comparable to estimates using two other whole-farm models (Integrated Farm System Model and Holos model). Variation was observed among models both in estimating whole-farm GHG emissions and the relative contribution of the different sources in the production system. Overall, whole-farm models are required to explore management options that will mitigate GHG emissions and promote best management practices. However, for full assessment of the production system, other benefits of the system (e.g., carbon sequestration, ecosystem services), which are not part of current whole-farm models, must be considered.

The research describes an optimized waste-to-energy technology that utilizes agricultural residues for renewable energy, while reducing global methane emissions and maintaining food security. Laboratory-, pilot- and farm-scale anaerobic batch digesters were evaluated to enhance methane production from the anaerobic digestion of untreated rice straw in dry conditions using a novel co-digestion approach. An existing farm-scale biogas plant loaded with rice straw and piggery wastewater produced 295 MWh in a 422-day digestion cycle. The long acclimation period (approximately 200 days) and low biogas yield (181 LCH4/kgVS) could be enhanced by adding anaerobic sludge from the pulp and paper mill treatment process. In a laboratory setting, the addition of the sludge resulted in a specific methane yield of 335 LCH4/kgVS within 92 days. Hydrolysis of the straw was accelerated, and stable conditions were observed in terms of pH, alkalinity and nutrients. Similar improvements were demonstrated in pilot-scale digesters (1 m3) - a specific methane yield of 231 LCH4/kgVS was achieved in a 93-day digestion cycle with the sludge compared to 189 days without the sludge. Insufficient mixing within the pilot-scale system caused lower overall methane yields than those obtained in the laboratory-scale digesters. If sufficient mixing and mesophilic conditions are maintained within the farm-scale system, the co-digestion of rice straw with pig wastewater and paper mill sludge (wet weight ratio of 1:1.25:0.5) has the potential to reduce the retention time to three months (versus 422 days) and increase methane yields to over 300 LCH4/kgVS

O presente trabalho teve como principal objetivo avaliar o desempenho de um reator anaeróbio híbrido (RAnH) em escala piloto no tratamento da fração líquida de estrume bovino, visando à remoção de matéria orgânica e à produção de biogás. O RAnH estudado apresentava volume total 6,2 m3 e foi composto por manta de lodo, na qual a biomassa encontrava-se suspensa, e leito fixo, no qual utilizou-se Biobob® como material suporte para imobilização da biomassa. Para dar suporte a partida do RAnH, a água residuária em estudo foi submetida a teste de biodegradabilidade visando avaliar a influencia da inoculação do reator no potencial de biodegradabilidade e de produção de metano. No teste, a inoculação mostrou-se adequada, apresentando biodegradabilidade de 57 ± 4% e produção de metano de 344 ± 26 mL CH4. g SV-1. A operação do RAnH foi realizada por 260 dias e foi dividida em duas etapas: etapa inicial, na qual se procedeu a adequação da operação do reator, e etapa experimental, na qual se avaliou o desempenho do reator perante o aumento progressivo da carga orgânica aplicada. Durante a operação do reator foi alcançada a aplicação de COV bruta total de 25,50 ± 2,53 kg DQO.m-3.d-1 e a COV solúvel total de 7,69 ± 0,02 kg DQO.m-3.d-1, sendo atingido o TDH de 1,27 ± 0,004 d. O RAnH apresentou eficiência de remoção média de 65 ± 4 % durante a etapa experimental. A produção média de metano foi de 0,310 ± 0,095 m3 CH4. m-3.d-1 e 0,098 ± 0,018 m3 CH4. kg SVad, com 89 ± 3% de metano na composição do biogás. O potencial de produção de energia elétrica estimada com base no reaproveitamento metano produzido no RAnH foi de 0,89 kWh.m-3. O aproveitamento desta energia em sistema com vazão de 3.500 m3.d-1 geraria energia suficiente para abastecer 615 residências e uma economia mensal de R$ 11.835,09.
The main objective of this work was to evaluate the performance of a pilot-scale anaerobic hybrid reactor (RAnH) in the treatment of the liquid fraction of dairy manure, in order to remove organic matter and produce biogas. The applied RAnH had a total volume of 6.2 m3 and was composed of sludge blanket, in which the biomass was suspended, and fixed bed, in which Biobob® was applied as a support material for biomass immobilization. In order to support RAnH startup, the wastewater under study was submitted to a biodegradability test to evaluate the influence of reactor inoculation on the biodegradability potential and methane production potential. In the test the inoculated condition presented appropriate biodegradability (57 ± 4%) and higher methane production potential (344 ± 26 mL CH4.gVS-1). The RAnH operation was performed for 260 days and was divided into two stages: initial stage, in which the reactor operation was adjusted, and the experimental stage, in which the reactor performance was evaluated with the progressive increase of the applied organic load. During the reactor operation was reachead total raw VOC application of 25.50 ± 2.53 kg COD m-3.d-1 and total soluble VOC of 7.69 ± 0.02 kg COD m-3.d-1, with the HRT of 1.27 ± 0.004 d. The RAnH presented average removal efficiency of 65 ± 4% during the experimental stage. The average methane production was 0.310 ± 0.095 m3 CH4.m-3.d-1 and 0.098 ± 0.018 m3 CH4. kg VSad, with 89 ± 3% of methane in the biogas composition. The potential of electric energy production estimated based on the reuse of the methane produced in the RAnH was 0,89 kWh.m-3. The use of this energy in a system with a flow of 3,500 m3.d-1 would generate sufficient energy to supply 615 homes and a monthly saving of R$ 11,835.09.

With the expansion of the Tillamook Creamery, in Tillamook Oregon, to double or more its cheese production, the demand for milk presents an economic opportunity for the member dairies of the Tillamook County Creamery Association. Before area dairies can expand their herd size to increase milk production for the creamery, the problem of manure waste management and pollution control must be solved. This study considers the technical and economic feasibility of developing a centralized waste treatment and methane generation facility to treat manure generated by Tillamook County dairies. A computer program modeling animal waste anaerobic digester design served as the basis for generating cost and production estimates for several hypothetical scenarios assuming input data specific to the Tillamook situation. A follow up study was also made to determine the variability of the potential ultimate methane yield of manures from Tillamook dairies. This study indicates that the proposed system is technically feasible. The study estimates that a comprehensive treatment system could cost dairymen from $70 to $100 per cow per year to start, but the economic feasibility improves as more manure is treated and more dairies participate. A full scale system has the potential to break even economically from the sale of electricity produced by a 5 megawatt methane powered generator. With the marketing of treated solids as a high grade fertilizer the system could gross a return of $1 to $75 per cow per year, depending on the scenario.
Graduation date: 1992

The aim of the study was to determine the effects of different dietary fibre levels on methane production and growth performance of Bonsmara and Nguni steers. Nine Bonsmara and nine Nguni male weaners aged 8 – 9 months were used for the study. On arrival, animals were fed Eragrostis curvula hay on ad libitum basis for the first 14 days as part of adaptation to the feedlot environment. Animals were gradually adapted to treatments to prevent metabolic disorders. The study was conducted as a 3 x 3 x 2 factorial experiment. Treatments were allocated in a completely randomised design. Data were submitted to analysis of variance (ANOVA). Student's t-LSD was calculated at the 5 % level (P < 0.05) to compare treatment means for significant effects. Feeding a diet that is low in crude fibre content had significantly increased (P < 0.05) growth performance in both breeds. Bonsmara steers had significantly higher growth performance (P < 0.0001) across all treatment diets compared to Nguni steers. High rumen total microbial count was observed in animals that received diets with low crude fibre. Reducing dietary crude fibre resulted in reduced methane production. Low dietary fibre showed low rumen archaea counts. There was a positive correlation between rumen pH and methane emission for both Bonsmara (R2 = 0.9105) and Nguni (R2 = 0.9517) steers. However, a negative correlation was observed between rumen total microbial count and feed conversion ratio for both Bonsmara (R2 = 0.8286) and Nguni (R2 = 0.7208) steers. The low rumen archaea counts were detected from the Bonsmara. Feeding diets with low fibre levels is recommended for improving growth performance and reducing methane production for Bonsmara and Nguni steers.
Agriculture and  Animal Health
M. Sc. (Agriculture)

The use of off-farm materials as amendments in anaerobic digestion of manure is an interesting option due to the benefits of boosting biogas production, and making the process more economical for the farmer. The addition of varying amounts of glycerol, which is a by-product of biodiesel production, was used as an amendment to anaerobic digestion of hog manure in lab-scale tests. The use of 2% glycerol produced the greatest amount of methane and biogas, however stabilization time was high, and the digestion of nutrients in the manure decreased. The addition of 4% glycerol resulted in an overloading of COD and digester failure. The addition of 1% glycerol resulted in a doubling of the methane and biogas production and the acclimation period was quite short, while the effluent quality remained good. There were no detrimental effects of using crude glycerol observed compared to using pure glcyerol. Batch tests also showed that smaller additions of glycerol (0.5%, 1%) produced the highest methane yields and were recommended as good co-substrates for anaerobic digestion with hog manure.
February 2009

碩士
國立中央大學
化學工程與材料工程研究所
99
Because the sharp rise in oil prices in recent years, and let the biomass energy has been re-valued(biomethane、biohydrogen、bioethanol、biodiesel), and rising environmental awareness of agricultural waste recycling is also increasing emphasis. Therefore, it is a very worthy subject of study. In this study investigated the methane production by anaerobic co-digestion swine manure with rice straw. Mainly the parameter are temperature(40 ℃、55℃) and inoculum(improved number 1、improved number 2、improved number 3) to investigated the effect of methane production, and find the optimal operating condition to produce methane by anaerobic digestion with above two experiments. Finally,investigated the effect of swine manure(SW) with rice straw(RS) at three different SW to RS volatile solid ratio of 3:1, 1:1, 1:3 by optimal operating condition. The results indicate that, the cumulative methane production was 3328.49 54.51 mL under the condition of temperature at 55℃ and add inoculum of improved number 3, and the cumulative methane production was the best. Cumulative methane production was 3745.11 79.54 mL under volatile solid ratio at 3:1 was the best in the experiment of different volatile solid ratio.