Dissertations / Theses on the topic 'Expression study'
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Hettle, S. J. H. "Expression of TN1/3 transposase." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375435.
Full textChristine, Deborah. "The teaching of children's artistic expression." Thesis, The University of Arizona, 1988. http://hdl.handle.net/10150/276714.
Full textWong, Hung-lai. "Gene expression study in ovary cancer." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B4073819X.
Full text黃虹麗 and Hung-lai Wong. "Gene expression study in ovary cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B4073819X.
Full textRODRIGUES, PAULA SALGADO LUCENA. "EXPRESSIVE TALKING HEADS: EXPRESSIVE TALKING HEADS: A STUDY ON SPEECH AND FACIAL EXPRESSION IN VIRTUAL CHARACTERS." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2002. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=6525@1.
Full textFUNDAÇÃO PADRE LEONEL FRANCA
A face humana é interessante e desafiadora acima de tudo pela sua familiaridade. Essencialmente, é a parte do corpo utilizada para reconhecer indivíduos. Assim como a face, a fala é um importante instrumento na forma de comunicação do ser humano. Através da fala é possível externar pensamentos e, muitas vezes, ela indica o estado de ânimo em que uma pessoa se encontra. Juntos, fala e face são os principais elementos de interatividade entre os seres humanos. Contudo, reproduzir com naturalidade e fidelidade as peculiaridades destes dois elementos no universo computacional não é uma tarefa simples, constituindo-se em tópicos de pesquisa em diversas áreas, em particular na animação facial. Entre os diversos tipos de sistemas de animação facial, destacam-se como diretamente relacionados a este trabalho aqueles que envolvem a sincronização da fala de um personagem com a animação da sua face. Sistemas desse tipo são conhecidos como talking head ou talking face. Para o desenvolvimento de um sistema talking head, é necessário identificar as possíveis abordagens para a modelagem dos dois elementos básicos: fala e face. Os modelos utilizados irão influenciar não apenas a maneira como a animação é conduzida, mas a própria forma de interatividade do sistema. Uma contribuição importante deste trabalho é o estudo das possíveis abordagens e a proposta de uma taxonomia para a classificação de sistemas talking head. A partir da taxonomia proposta e fazendo uso de uma determinada abordagem para cada parâmetro analisado, foi desenvolvida uma aplicação que recebe como entrada um texto contendo a fala e anotações de expressividade e gera como saída, em tempo real, a animação de um personagem virtual enunciando o texto de entrada com o áudio e os movimentos faciais sincronizados. O sistema desenvolvido, denominado Expressive Talking Heads, explora a naturalidade da animação facial e ao mesmo tempo busca oferecer ao usuário uma interface com interatividade em tempo real. O Expressive Talking Heads pode ser executado tanto no modo isolado (stand alone) como acoplado a navegadores para a web, tendo sido projetado e desenvolvido com a preocupação de oferecer uma solução independente da plataforma e do sistema operacional utilizados.
The human face is interesting and challenging mainly because of its familiarity. Essentially, it is the part of the human body that is used to recognize individuals. As well as the face, the speech is an important instrument for human communication, allowing the exteriorization of thoughts and the definition of emotions. Together, speech and face are the main elements of interactivity among human beings. However, the natural and faithful reproductions of the pecularities of these elements in the computational universe is not a simple task, constituting topics of the research in the diverse areas, particularly in facial animation. Among the diverse types of facial animation systems developed, those that involve the facial animation of the virtual character combined with speech synchronization are distinguished as directly related to this work. These kinds of systems are known as talking head or talking face. Fot the development of a talking head system, it is necessary to identify the possible approaches for the speech and face modeling. The models used will influence not only the way that the animation is performed, but it will also affect the system´s interactivity. An important contribution of the present master thesis is the study of several possible approaches for the main elements and the proposal of taxonomy for the classification of the talking head systems. From the proposed taxonomy and making use of one approach for each analyzed paramenter, an application was developed that receives as input a text composed by the character´s speech and genus, language and emotion parameters, and it generates as output, in real time, the animation of a virtual character uttering the input text with speech synchronization and expressiveness. The system developed, called Expressive Talking Heads, explores the naturalness of facial animation and it seeks to offer the user a real- time interactivity interface. The Expressive Talking Heads system can run as a stand-alone applicattion or connected to web browsers. It was designed and developed to provide a platform and operating system independent solution.
Guo, Ran. "Subcloning, Expression, and Enzymatic Study of PRMT5." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/biology_theses/26.
Full textDevlin, Andrea. "A study of CYP1B1 expression in tumourigenesis." Thesis, University of Ulster, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494336.
Full textFan, Wai-leong, and 樊偉亮. "Study of gene expression on ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44659039.
Full textVillette, Stephane. "Molecular study of selenoprotein in gene expression." Thesis, University of Newcastle Upon Tyne, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391327.
Full textSteel, Muriel C. "A study of muscarinic receptor gene expression." Thesis, University College London (University of London), 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314798.
Full textYuwono, Triwibowo. "A study of actinidin expression in yeast." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/34380.
Full textAllen, Rebecca Louise. "A study of gene expression in Pseudomonas." Thesis, University of Leicester, 1989. http://hdl.handle.net/2381/35185.
Full textWhite, David Robert. "Studies on the acquisition, expression and disruption of mammalian sperm motility." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/27046.
Full textGonçalves, Ângela. "RNA sequencing for the study of gene expression regulation." Thesis, University of Cambridge, 2012. https://www.repository.cam.ac.uk/handle/1810/265548.
Full textAli, Norryai A. "Expression and secretion of OXA-2 beta-lactamase by Streptomyces lividans." Thesis, University of Surrey, 1986. http://epubs.surrey.ac.uk/847196/.
Full textToufighi, Kiana 1980. "Integrative study of gene expression and protein complexes." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/380907.
Full textEn las últimas décadas, la emergente vista integrativa de la célula ha triunfado sobre el paradigma histórico: ‘un gene/una proteína/una función’. Esto es ilustrado por los efectos biológicos opuestos de proteínas regulatorias clave en cultivos celulares inmortalizados frente a primarios e in vitro frente a in vivo. El tema persistente en este disertación es la integración de un amplio set de datos para estudiar los distintos contextos celulares. En primer lugar, utilizamos los datos de expresión génica obtenidos de células madre epidérmicas para descubrir las ondas de transcripción expresadas en sintonía con los genes conocidos de los ritmos circadianos. En este estudio demostramos que las respuestas de las células madres a las señales de proliferación/diferenciación dependen de hora del día y el tiempo circadiano es importante para la homeostasis de la piel. Posteriormente, combinamos estos datos de expresión con la información estructural de proteínas y complejos proteicos para describir la regulación temporal de complejos durante el proceso de diferenciación. Por último, mostramos que los complejos de proteínas humanos están compuestos de un ‘núcleo’ estable y una 'periferia' plástica cuya expresión específica de tejido celular permite que los complejos de proteínas funcionen de una manera dependiente del contexto.
De, las Heras Rachel, and n/a. "Neuronal Differentiation: A Study Into Differential Gene Expression." Griffith University. School of Biomolecular and Biomedical Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040225.161725.
Full textSangaralingham, Sasantha Jeson. "Study of the gene expression during cardiac growth." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ55929.pdf.
Full textWakeley, Philip Robert. "A study of maize male gametophytic gene expression." Thesis, Royal Holloway, University of London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261723.
Full textChoudhury, Tanzeem Khalid 1975. "FaceFacts : study of facial features for understanding expression." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/61109.
Full textIncludes bibliographical references (p. 79-83).
by Tanzeem Khalid Choudhury.
S.M.
Varanka, T. (Tuomas). "A comparative study of facial micro-expression recognition." Bachelor's thesis, University of Oulu, 2019. http://jultika.oulu.fi/Record/nbnfioulu-201909282947.
Full textMeyer, Eric C. "A visual scanpath study of facial affect recognition in schizotypy and social anxiety." Diss., Online access via UMI:, 2005.
Find full textMullarkey, K. "Gene expression in the salivary gland of the blowfly, (Calliphora vomitoria), larva." Thesis, University of Exeter, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382866.
Full textBohlin, Stina. "Meaningless movement or essential expression : A study about gestures." Thesis, Luleå tekniska universitet, Institutionen för ekonomi, teknik, konst och samhälle, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-85247.
Full textSpauwen, Janneke, Lydia Krabbendam, Roselind Lieb, Hans-Ulrich Wittchen, and Os Jim van. "Does urbanicity shift the population expression of psychosis?" Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-108748.
Full textBambrough, Marilyn Edna. "An Artful Habitat:Creating an Environment for Divergent Expression." BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/8798.
Full textLi, Zhi 1959 Dec 23. "Cloning, expression and functional study of human hepadnaviral polymerase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0010/NQ59620.pdf.
Full textWhitehouse, Adrian. "An inducible expression system to study baculovirus gene function." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260142.
Full textPrice, Rebekah. "Study of expression of multigene families in Plasmodium falciparum." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270241.
Full textPappas, Beverly. "Mechanistic Study of p23-Mediated Aryl Hydrocarbon Receptor Expression." Scholarly Commons, 2018. https://scholarlycommons.pacific.edu/uop_etds/3131.
Full textTodd, C. B. "Septin 6 : A Study of Splicing, Expression and Stability." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501611.
Full textTorres, FÃbio Fernandes. "The gerund in future tense expression: a sociofunctionalism study." Universidade Federal do CearÃ, 2009. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3814.
Full textFundaÃÃo de Amparo à Pesquisa do Estado do CearÃ
This research deals with the variation of future tense with gerund forms in the Portuguese spoken in Fortaleza, from data of speakers of three different professions, collected by means of sociolinguistic interviews: salesmen, teachers and telemarketing workers. This phenomenon presents six variants: a) the imminent periphrastic simple future, b) the imminent periphrastic extended future - that compose the imminent future variable; c) the medium periphrastic simple future, d) the medium periphrastic extended future - that compose the medium future variable; e) the resultative periphrastic simple future, and f) the resultative periphrastic extended future - that compose the resultative future variable. We also consider a definition of gerundismo based on form, aspect, modality and temporal criteria. The theoretical referential is composed by the association of postulates of the Sociolinguistics Analysis and the Linguistic Functionalism, resulting in the theoretical configuration of the Sociofuncionalismo, considered by Tavares (2003). The analysis is performed in four stages: in the first one, we analyze the variants of imminent future; after that, the variants of medium future; later, the variants of resultative future and, finally, a section is dedicated for analysis of the variant called gerundismo. Results confirm that the phenomenon under study is influenced by factors of distinct nature: social factors, such as sex and profession of speakers and linguistic factors as type of the verb (auxiliary or modal verbs) and presence of one mark of future tense.
Esta dissertaÃÃo trata da variaÃÃo de tempo futuro no portuguÃs falado em Fortaleza codificado por perÃfrases gerundivas, a partir de dados de fala de informantes de trÃs diferentes Ãreas de atuaÃÃo, coletados por meio de entrevistas sociolinguÃsticas: vendedores, professores e operadores de telemarketing. Esse fenÃmeno apresenta seis variantes: a) futuro iminente perifrÃstico simples, b) futuro iminente perifrÃstico estendido â que compÃem a subvariÃvel futuro iminente; c) futuro mÃdio perifrÃstico simples, d) futuro mÃdio perifrÃstico estendido â que compÃem a subvariÃvel futuro mÃdio; e) futuro resultativo perifrÃstico simples e f) futuro resultativo perifrÃstico estendido â que compÃem a subvariÃvel futuro resultativo. Trata-se tambÃm da variante denominada gerundismo para a qual propomos uma definiÃÃo baseada em critÃrios tais como forma, aspecto, modalidade e natureza temporal. O referencial teÃrico à composto pela associaÃÃo de postulados da SociolinguÃstica Variacionista e do Funcionalismo LinguÃstico, resultando na configuraÃÃo teÃrica do Sociofuncionalismo, proposto por Tavares (2003). A anÃlise à feita em quatro etapas: na primeira, analisamos as variantes de futuro iminente; em seguida, as variantes de futuro mÃdio, depois, as variantes de futuro resultativo e, por Ãltimo, à dedicada uma seÃÃo para anÃlise da variante denominada gerundismo. Os resultados confirmam que o fenÃmeno em estudo à influenciado por fatores de uma natureza distinta: fatores sociais, tais como o sexo e a profissÃo dos falantes e fatores linguÃsticos como o tipo do verbo (verbos auxiliares ou modais) e presenÃa de uma marca de tempo futuro.
Tang, Mei Kuen. "Applications of the subtractive hybridization method to study gene expression in rat liver after cadmium exposure." HKBU Institutional Repository, 1994. https://repository.hkbu.edu.hk/etd_ra/39.
Full textParker, Sydney Lawrence. "Temporality and its expression in the interlanguage of adult learners of Welsh." Thesis, University of Wales Trinity Saint David, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683050.
Full textJia, Yizhen, and 贾亦真. "Bioinformatics study of the lineage and tissue specificity of genes and gene expression." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45540652.
Full textLin, Shyr-Yeu 1962. "Genetically engineered mouse models for the study of follistatin biology." Monash University, Institute of Reproduction and Development, 2003. http://arrow.monash.edu.au/hdl/1959.1/5739.
Full textTsiga, I. A. "To tell freedom : A study of black South African autobiography." Thesis, University of Essex, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377927.
Full textBall, Sarah Elizabeth. "A study of c-fms in myeloid leukaemias." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252976.
Full textSpauwen, Janneke, Lydia Krabbendam, Roselind Lieb, Hans-Ulrich Wittchen, and Os Jim van. "Does urbanicity shift the population expression of psychosis?" Technische Universität Dresden, 2004. https://tud.qucosa.de/id/qucosa%3A26767.
Full textBailey, Paul Charles. "A molecular study of dormancy-breaking in seeds of Trollius ledebouri." Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260658.
Full textEffio, Pedro Jorge Chimoy. "Estudo sobre a expressão dos genes de alfa amilase de Bacillus stearothermophilus e de anopheles merus em células de bactéria, levedura e inseto." Universidade de São Paulo, 2001. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-25102018-171719/.
Full textThe alpha amylase A1 gene was isolated trom a genomic library of lambda EMBL3 made with DNA from the primitve Díptera Anopheles merus (Díptera, Nematocera, Cuclicoidea). This gene was partialy sequenced, characterized by standard restriction enzyme cleavage, and cloned into the pIBI24 plasmid by Pernasetti (1991). Here we present the expression of this gene in: bacteria (Escherichia coli AD494), insect cell (Spodoptera frugiperda) and yeast (Pichia pastoris). It was shown that only in Spodoptera frugiperda cells the protein display enzymatic activity. The presence of the A1 product in the extracelullar culture media was tested in Escherichia coli AD494 using the vector pRSETc. To this end, the gene sequence containing its own signal peptide was inserted in frame into the vector. Expression without a signal peptide was conducted using the pAE2 vector, a construct derived from pRSETc. In another construct, the A1 gene was inserted in frame after the promoter sequence and the signal peptide of the A2 gene (alpha amylase Bacillus stearothermophilus) using the pIBI24-Bst vector. Although the E.coli AD494 cells transformed with these constructs expressed the enzyme, no enzyme activity was detected. A1 was not secreted, either with its own signal peptide or with that of amylase A2. Expression of the A1 gene in yeast (Pichia pastoris) was conducted using the pPic9 vector, wich allows intracellular expression or secretion of this protein. The gene was amplified by PCR with forward primers, so as to amplyfy the gene sequence with or without the signal peptide. The gene containing the signal peptide was constructed with different Kozak sequences preceeding the ATG codon. For extracellular expression, the signal peptide of the pPic9 alpha factor was used. The results showed that the enzyme is expressed , but remained within the cell and do not display activity. The same constructs of the A1 gene made for P. pastoris were inserted in Baculovirus to infect Spodopera frugiperda cells. In this system, the amylase was successfully expressed and display enzymatic activity, mainly in the extracellular supernatant, using constructs of the gene with own signal peptide or with the signal peptide for amylase of Zabrotes subfasciatus. The gene A2 was expressed in the Baculovirus/Spodoptera system and the yeast P. pastoris using constructs containing the signal peptide of alpha amylase of Z. subfasciatus, the protein was secreted with activity.
Morten, Karl J. "A study of La France disease in Agaricus bisporus." Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254387.
Full textSodekawa, Hiromi. "Enchi Fumiko : a study in the self-expression of women." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28285.
Full textArts, Faculty of
Asian Studies, Department of
Graduate
Han, Jiahuai. "Study of the regulation of cachectin/tumor necrosis factor expression." Doctoral thesis, Universite Libre de Bruxelles, 1990. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213139.
Full textBaken, Kirsten Annika. "Immunotoxicogenomics gene expression profiling as a tool to study immunotoxicity /." [Maastricht] : Maastricht : Universitaire Pers Maastricht ; University Library, Universiteit Maastricht [host], 2007. http://arno.unimaas.nl/show.cgi?fid=9329.
Full textStern, Kalyn Michiko. "Immunohistological study of sorting nexin 27 expression in rat brain." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p1453421.
Full textTitle from first page of PDF file (viewed July 14, 2008). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 88-94).
Grant, A. "The hippocalcin gene : a study in neuron-specific gene expression." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599607.
Full textLeung, Cheuk-man, and 梁卓文. "A study of BARX2 expression in esophageal squamous cell carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B47560460.
Full textpublished_or_final_version
Pathology
Master
Master of Medical Sciences
曾騰霈. "Study on Robot Facial Expression." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/70225260118183508943.
Full text建國科技大學
自動化工程系暨機電光系統研究所
99
The facial expressions of a robot were designed to have the robot look friendly. Propeller servo controller and Visual Basic Windows were applied for controlling actuators. The computer programs developed in this research may drive eyelids, mouth, and brows individually or simultaneously to create the facial expressions that include happiness, anger, depression and gladness. The results of this study made robots personified.
"Study of BRE expression and regulation." 2006. http://library.cuhk.edu.hk/record=b5896531.
Full textThesis (M.Phil.)--Chinese University of Hong Kong, 2006.
Includes bibliographical references (leaves 164-179).
Abstracts in English and Chinese.
Chapter Chapter one: --- Introduction --- p.4
Chapter 1.1 --- Introduction of BRE --- p.4
Chapter 1.1.1 --- Discovery of BRE --- p.4
Chapter 1.1.2 --- cDNA sequence and amino acids sequence of BRE --- p.4
Chapter 1.1.3 --- BRE expression level in Human and rat organs --- p.5
Chapter 1.1.4 --- Expression of Human and mouse BRE in multiple isoforms --- p.6
Chapter 1.1.4.1 --- BRE isoforms in Human --- p.6
Chapter 1.1.4.2 --- BRE isoforms in mouse --- p.6
Chapter 1.1.5 --- mRNA level of BRE upon stress --- p.7
Chapter 1.1.6 --- BRE and steroidogenesis --- p.8
Chapter 1.1.7 --- BRE and p55 tumor necrosis factor α (TNF) receptor --- p.9
Chapter 1.1.8 --- BRE and NFkB activity --- p.9
Chapter 1.1.9 --- Anti-apoptotic effect of BRE --- p.10
Chapter 1.1.10 --- BRE enhances the growth of tumor cells --- p.12
Chapter 1.1.11 --- BRE and its ubiquitination activity --- p.12
Chapter 1.1.12 --- Regulation of Prohibitin and p53 expression and proliferation by BRE --- p.13
Chapter 1.2 --- Regulation of transcription --- p.15
Chapter 1.2.1 --- Cis-acting elements --- p.17
Chapter 1.2.1.1 --- The TATA box --- p.18
Chapter 1.2.1.2 --- The GC box and CAAT box --- p.18
Chapter 1.2.1.3 --- The initiator (Inr) --- p.19
Chapter 1.2.1.4 --- CpG islands --- p.20
Chapter 1.2.2 --- Trans- acting protein factors --- p.21
Chapter 1.2.2.1 --- Zinc finger domain --- p.21
Chapter 1.2.2.2 --- Basic helix-turn-helix domain (bHLH) --- p.22
Chapter 1.3 --- Hypothesis and Objectives --- p.23
Chapter Chapter two: --- Materials and Methods --- p.25
Chapter 2.1 --- Materials --- p.25
Chapter 2.1.1 --- Primers used in polymerase chain reaction (PCR) and sequencing --- p.25
Chapter 2.1.2 --- DNA clones used in the studies --- p.26
Chapter 2.1.3 --- Materials for DNA manipulation --- p.27
Chapter 2.1.4 --- Materials for protein manipulation --- p.28
Chapter 2.1.5 --- Antibodies --- p.28
Chapter 2.1.6 --- Chemical used in treatments --- p.29
Chapter 2.1.7 --- Kits --- p.29
Chapter 2.1.8 --- Culture media and reagents --- p.30
Chapter 2.1.9 --- Instrumentation --- p.30
Chapter 2.1.10 --- Bacterial strain used for transfection and cloning --- p.31
Chapter 2.2 --- Methodologies --- p.36
Chapter 2.2.1 --- Cell culture --- p.36
Chapter 2.2.1.1 --- Monolayer cells --- p.36
Chapter 2.2.1.2 --- Suspension cell --- p.36
Chapter 2.2.2 --- Identification of the transcriptional start site (TSS) of BRE by RNA ligase- mediated rapid amplification of 5,and 3,cDNA ends (RLM-RACE) --- p.37
Chapter 2.2.3 --- Preparation of the 5' untranslated region (UTR) fragments of BRE --- p.39
Chapter 2.2.3.1 --- Polymerase chain reaction (PCR) with Taq polymerase --- p.39
Chapter 2.2.3.2 --- Polymerase chain reaction (PCR) with PhusiońёØ high-fidelity DNA.… --- p.40
Chapter 2.2.4 --- Construction of the reporter constructs --- p.42
Chapter 2.2.5 --- Cell transfection --- p.42
Chapter 2.2.6 --- Dual-luciferase reporter assay --- p.43
Chapter 2.2.7 --- Western blotting --- p.44
Chapter 2.2.8 --- Cell cycle analysis by flow cytometry --- p.45
Chapter 2.2.9 --- BRE antibody production --- p.43
Chapter Chapter Three: --- Identification of transcriptional start sites and promoter region for BRE --- p.52
Chapter 3.1 --- Identification of the transcriptional start sites for BRE --- p.52
Chapter 3.2 --- Computational analysis of the 5' region of BRE --- p.57
Chapter 3.2.1 --- Putative transcriptional factor binding sites --- p.57
Chapter 3.2.2 --- CpG island --- p.58
Chapter 3.3 --- Identification of BRE promoter --- p.64
Chapter Chapter Four: --- Characterization of transcriptional regulation of BRE --- p.70
Chapter 4.1 --- Regulation of BRE promoter by genotoxic stimuli and retinoic acid --- p.71
Chapter 4.1.1 --- Etoposide --- p.71
Chapter 4.1.2 --- 4-nitroquinoline-l -oxide (4NQO) --- p.79
Chapter 4.1.3 --- Retinoic acid (RA) --- p.87
Chapter 4.2 --- Regulation of BRE promoter by p53 protein and gamma irradiation --- p.90
Chapter 4.2.1 --- Co-transfection with p53 plasmid --- p.90
Chapter 4.2.2 --- Gamma irradiation (y irradiation) --- p.96
Chapter 4.2.2.1 --- γ irradiation treatment of HeLa cells --- p.96
Chapter 4.2.2.2 --- γ irradiation treatment of Balb/c 3T3 cells --- p.99
Chapter 4.3 --- Regulation of BRE promoter by BRE --- p.103
Chapter 4.3.1 --- Co-transfection with V5-tagged BRE (GS-BRE) --- p.103
Chapter 4.3 2 --- Co-transfection with untagged BRE (pcDNA3-BRE) --- p.107
Chapter 4.4 --- Regulation of BRE promoter by culture condition --- p.110
Chapter 4.4.1 --- Cell density --- p.110
Chapter 4.4.2 --- Serum deprivation --- p.114
Chapter 4.5 --- Regulation of BRE promoter by kinase inhibitors --- p.120
Chapter Chapter Five: --- BRE and cell cycle analysis --- p.127
Chapter 5.1 --- Cell synchronization in G1 phase by aphidicolin (APC) --- p.127
Chapter 5.1.1 --- Flow analysis --- p.128
Chapter 5.1.2 --- Luciferase reporter assay --- p.128
Chapter 5.1.3 --- Western blot analysis --- p.129
Chapter 5.2 --- Cell synchronization in G2/M phase by colchicine (COL) --- p.137
Chapter 5.2.1 --- Flow analysis --- p.137
Chapter 5.2.2 --- Luciferase reporter assay --- p.137
Chapter 5.2.3 --- Western blot analysis --- p.138
Chapter 5.3 --- Cell cycle analysis of the treatments investigated by luciferase assays --- p.144
Chapter Chapter Six: --- Discussion --- p.149
Chapter 6.1 --- Study of BRE expression --- p.149
Chapter 6.2 --- Study of BRE regulation --- p.154
Chapter 6.3 --- Conclusion --- p.163
Reference --- p.164
Appendix (Raw data and statistical information of luciferase assays)