Relevant bibliographies by topics / Expression à la surface de la levure

Academic literature on the topic 'Expression à la surface de la levure'

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Published: 7 July 2024
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  2. Dissertations / Theses
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  4. Book chapters
  5. Conference papers
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Journal articles on the topic "Expression à la surface de la levure":

1

Köster, B., and M. Strand. "Schistosoma mansoni: immunolocalization of two different fucose-containing carbohydrate epitopes." Parasitology 108, no. 4 (May 1994): 433–46. http://dx.doi.org/10.1017/s0031182000075995.

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SUMMARYWe have used two monoclonal antibodies, 128C3/3 and 504B1, to immunolocalize their carbohydrate epitopes in different developmental stages of Schistosoma mansoni. Both epitopes contain fucose: mAb 128C3/3, as we have shown previously, recognizes fucose in a novel, possibly internal linkage (Levery et al. 1992) while mAb 504B1, as we show here, bound to the Lex epitope, which contains fucose α1 → 3 linked to N-acetyl-glucosamine. The tissue expression of these epitopes was strikingly different and both elicit an immune response in infected hosts. The mAb 128C3/3-defined epitope was exposed on the surface of all larval stages but not on adult worms; however, it was found in the excretory system of adult worms of both sexes. In contrast, surface expression of the Lex epitope was initiated after the transformation of cercariae to schistosomula and was maintained throughout the adult life in both sexes.
2

Creson, Jennifer R., Andy A. Lin, Qun Li, David F. Broad, Margo R. Roberts, and Stephen J. Anderson. "The Mode and Duration of Anti-CD28 Costimulation Determine Resistance to Infection by Macrophage-Tropic Strains of Human Immunodeficiency Virus Type 1 In Vitro." Journal of Virology 73, no. 11 (November 1, 1999): 9337–47. http://dx.doi.org/10.1128/jvi.73.11.9337-9347.1999.

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ABSTRACT We have investigated the ability of anti-CD28 antibody costimulation to induce resistance to macrophage (M)-tropic strains of human immunodeficiency virus type 1 (HIV-1) in vitro. Our results confirm the observations of Levine et al. (15) that stimulation of CD4 T cells with anti-CD3/anti-CD28 antibodies coimmobilized on magnetic beads renders the cells resistant to infection by M-tropic strains of HIV-1. The resistance was strongest when the beads were left in the cultures throughout the experiment. In contrast, stimulation of CD4 T cells with the same antibodies immobilized on the surface of plastic culture dishes failed to induce resistance and resulted in high levels of p24 production. This was true even if the cells were passaged continuously on freshly coated plates. If the beads were removed after initial stimulation, p24 production increased over time and produced a result intermediate to the other forms of stimulation. For beads-in, beads-out, and one-time plate stimulated cultures, resistance to infection correlated with down-regulation of CCR5 expression at the cell surface and with increased production of β-chemokines. However, cultures of CD4 T cells continuously passaged on anti-CD3/anti-CD28-coated plates produced large amounts of p24 despite decreased levels of CCR5 expression and increasing production of β-chemokines. Expression of the T-cell activation markers CD25 and CD69 and production of gamma interferon further supported the differences in plate versus bead stimulation. Our results explain the apparent contradiction between the ability of anti-CD28 antibody costimulation to induce resistance to HIV infection when presented on magnetic beads and the increased ability to recover virus from the cells of HIV-positive donors who are on highly active antiretroviral therapy when cells are stimulated by anti-CD3/anti-CD28 immobilized on plastic dishes.
3

Bagashev, Asen, Elena Sotillo, Glendon Wu, and Andrei Thomas-Tikhonenko. "The Importance of CD19 Exon 2 for Surface Localization: Closing the Ig-like Loop." Blood 126, no. 23 (December 3, 2015): 3433. http://dx.doi.org/10.1182/blood.v126.23.3433.3433.

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Abstract Background: CD19 is a near-universal surface marker of B-cell malignancies. Therefore, it is regarded as a target of choice for various immunotherapies, such as bi-specific T-cell engagers and chimeric antigen receptor-armed T-cells. Although the latter have proven remarkably effective in treating B-cell acute lymphoblastic leukemia (B-ALL), relapses caused by the loss of the CD19 surface epitopes are emerging as a major reason for treatment failure (Maude et al, 2014). However, the molecular mechanisms of epitope loss remain incompletely characterized. Recently, we have identified a novel alternatively spliced CD19 isoform lacking exon2 (Δex2 CD19) (Sotillo et al 2015). Although exon 2 encodes a single conserved N-linked glycosylation site (Asn-86) and two conserved cysteine residues (Cys-38 and Cys-97) responsible for the formation of a disulfide bond (Zhou et al 1991), the function and subcellular localization of the Δex2 isoform remained unknown. Our aim was to identify specific amino acids within exon 2 that are responsible for proper CD19 surface expression. Methods: CD19-null (ΔCD19) derivatives of Nalm6 and 697 B-ALL cell lines were generated using the CRISPR/Cas9 approach. We then reconstituted them with full-length and Δex2 CD19 isoforms, expressed either on their own or as CD19-GFP fusions. Additionally, CD19 N-terminus mutations (ΔSP [no signal peptide], N86A, C97A and N86A/C97A) were introduced in full-length CD19. Transduced cells were analyzed by flow cytometry, confocal microscopy, and western blotting. Glycosylation of the mutants was verified using treatment with swainsonine (Golgi glycosylation inhibitor) and an in vitro de-glycosylation assay. Results: As expected, deletion of the N-terminal signal peptide responsible for the endoplasmic reticulum translocation led to impaired surface expression. Surprisingly, deletion of exon 2 sequences had a similar effect: although up to 10% of Δex2 CD19 was found on the plasma membrane (where it enhanced pre-B-cell receptor signaling), the remainder was largely cytosolic. Furthermore, while the N86A substitution in full-length CD19 did not significantly affect its surface localization, substituting Cys-97 with Ala fully recapitulated the Δex2 cytosolic phenotype. In fact, the C97A mutant appeared to be stuck in the ER and never reach Golgi, since its electrophoretic mobility was not affected by swainsonine. In contrast, its glycosylation in ER was unperturbed, as evidenced by in vitro de-glycosylation assays. Conclusion: The immunoglobulin-like loop connecting Cys-38 and Cys-97 of CD19 is required for its plasma membrane localization. One possible mechanism is the N-termini-mediated interaction with CD81, which is known to be needed to transport CD19 to the cell surface (Matsumoto et al 1993). Maude SL, Frey, N, Shaw, PA, Aplenc R, Barrett DM, Bunin NJ, Chew A, Gonzalez VE, Zheng Z, Lacey SF, Mahnke YD, Melenhorst JJ, Rheingold SR, Shen A, Teachey DT, Levine BL, June CH, Porter DL, and Grupp SA. Chimeric antigen receptor T cells for sustained remissions in leukemia. N Engl J Med 2014 371:1507-1517. Sotillo E, Barrett D, Bagashev A, Black K, Lanauze C, Oldridge D, Sussman R, Harrington C, Chung EY, Hofmann TJ, Maude SL, Martinez NM, Raman P, Ruella M, Allman D, Jacoby E, Fry T, Barash Y, Lynch KW, Mackall C, Maris J, Grupp SA, and Thomas-Tikhonenko A. Alternative splicing of CD19 mRNA in leukemias escaping CART-19 immunotherapy eliminates the cognate epitope andcontributes to treatment failure. 2015 AACR Annual Meeting, Philadelphia. Zhou LJ, Ord DC, Hughes AL and Tedder TF, Structure and domain organization of the CD19 antigen of human, mouse, and guinea pig B lymphocytes. Conservation of the extensive cytoplasmic domain. J Immunol. 1991 147(4):1424-32 Matsumoto AK, Martin DR, Carter RH, Klickstein LB, Ahearn JM, and Fearon DT Functional dissection of the CD21/CD19/TAPA-1/Leu-13 complex of B lymphocytes. J Exp Med. 1993 178(4):1407-17. Disclosures No relevant conflicts of interest to declare.
4

Labroussaa, Fabien, Vincent Baby, Sébastien Rodrigue, and Carole Lartigue. "La transplantation de génomes." médecine/sciences 35, no. 10 (October 2019): 761–70. http://dx.doi.org/10.1051/medsci/2019154.

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Le développement de la génomique synthétique (GS) a permis l’élaboration d’outils et de méthodes innovantes permettant la synthèse, l’assemblage et la modification génétique précise de chromosomes bactériens complets. La raison principale de ce succès, ayant abouti à la création de la première cellule synthétique quasi-minimale JCVI-syn3.0, est l’utilisation de la levure Saccharomyces cerevisiae comme hôte temporaire d’accueil et de modification de ces génomes. Cependant, une autre technique a joué un rôle considérable dans le succès retentissant de ces travaux : la transplantation de génomes bactériens (TG). Cette technique, encore mal comprise, permet d’installer des génomes complets naturels ou synthétiques dans un contexte cellulaire favorable à leur expression et donner la vie. Une meilleure compréhension du processus de TG permettrait d’élargir l’ensemble des techniques de GS, appliquées actuellement quasi exclusivement à l’étude des mycoplasmes, à de nombreuses autres bactéries d’intérêt, y compris des bactéries génétiquement non-modifiables à ce jour.
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RAHAMIMOFF, HANNAH, XIAOYAN REN, CHAVA KIMCHI-SARFATY, SURESH AMBUDKAR, and JUDITH KASIR. "NCX1 Surface Expression." Annals of the New York Academy of Sciences 976, no. 1 (January 24, 2006): 176–86. http://dx.doi.org/10.1111/j.1749-6632.2002.tb04739.x.

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Käfer, G., A. Willer, W. D. Ludwig, A. Krämer, R. Hehlmann, and J. Hastka. "Intracellular expression of CD61 precedes surface expression." Annals of Hematology 78, no. 10 (October 21, 1999): 472–74. http://dx.doi.org/10.1007/s002770050601.

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7

Kanematsu, Takashi, Makoto Fujii, Hiroto Tanaka, Hisanori Umebayashi, and Masato Hirata. "Surface Expression of GABAA Receptors." Journal of Oral Biosciences 52, no. 4 (January 2010): 322–29. http://dx.doi.org/10.1016/s1349-0079(10)80012-x.

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8

Dunbar, Andrew, Min Lu, Mirko Farina, Young Park, Julie Yang, Dongjoo Kim, Abdul Karzai, et al. "Increased Interleukin-8 (IL8)-CXCR2 Signaling Promotes Progression of Bone Marrow Fibrosis in Myeloproliferative Neoplasms." Blood 136, Supplement 1 (November 5, 2020): 6–7. http://dx.doi.org/10.1182/blood-2020-138843.

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Introduction: Elevated pro-inflammatory cytokines are a hallmark feature of myeloproliferative neoplasms (MPNs). The pro-inflammatory cytokine interleukin-8 (IL8) is increased in patients with myelofibrosis (MF) and correlates with adverse outcome, including overall survival. Previously, the Levine/Fang labs identified increased IL8 secretion from individual CD34+ stem cells in a subset of MF patients. The role of IL8 and its cognate receptors CXCR1/2 in MF pathogenesis has not been delineated. Methods: Single-cell cytokine assays were performed on isolated CD34+ cells from 60 clinically annotated MPN patients (20 MF, 20 PV, 20 ET) using a previously described micro-chip platform (Kleppe et al, Can Disc 2013). 10 healthy donors (CD34+ cells from hip replacements) were used as controls. Integrated RNA-Seq and Assay for Transposase-Accessible Chromatin followed by next-generation sequencing (ATAC-Seq) was performed on CD34+ cells from MPN patients with and without expanded IL8 secreting clones for gene expression/chromatin accessibility analysis. To model the role of IL8-CXCR2 on fibrosis in vivo, the human MPLW515L transplant model (hMPLW515L) of MF was used. Specifically, wild-type (WT) murine bone marrow (Creneg-Cxcr2f/f; Cxcr2WT) or marrow lacking the CXCR2 receptor (VavCre-Cxcr2f/f; Cxcr2KO)were retrovirally infected with MSCV-hMPLW515L-IRES-GFP and transplanted into lethally irradiated WT recipient mice and monitored for disease. Blood counts, chimerism, and flow cytometry were assayed. Moribund mice were sacrificed and assayed for grade reticulin fibrosis and overall survival. Results: Single-cell cytokine assays confirmed an increased proportion of IL8-secreting CD34+ cells in MF patients (40%) in comparison to other MPN sub-types (10% PV/0% ET) (Figure 1A). MF patients with expanded IL8 secreting clones (defined as >50% of total CD34+ cells) had increased leukocytosis (p<0.0001), larger spleen sizes (p=0.0004), greater prevalence of constitutional symptoms (p=0.0084), and higher-grade reticulin fibrosis in marrow (Figure 1B) in comparison to MF patients without prevalent IL8 clones. IHC confirmed increased IL8 expression in marrow biopsies from 8/15 MF patients in comparison to 0/4 normal controls (Figure 1C), and high IL8 expression was also observed in MF splenic megakaryocytes (MKs) as well as in splenic stromal/endothelial cells not seen in normal spleen (Figure 1D). Integrated RNA-Seq/ATAC-Seq analysis of IL8-high MF patients confirmed up-regulation of IL8-CXCR2 signaling and enrichment in pro-inflammatory pathways (i.e TNFa, NFkB, etc) by GSEA, as well as increased expression/accessibility of pro-inflammatory genes S100A8 and S100A9-previously implicated in fibrosis development. Flow analysis of IL8-high MF CD34+ cells revealed enhanced surface expression of CXCR2 and its analog CXCR1, such that MF was characterized by increased IL8 ligand and receptor expression (Figure 1E) and coincided with enhanced NFkB pathway activity (Figure 1F). Consistent with this, colony forming assays of cultured MF CD34+ cells revealed enhanced colony output when cultured with IL8 compared to WT CD34+ cells-an effect ameliorated by co-treatment with the CXCR1/2 antagonist Reparixin (Figure 1G). In vivo, hMPLW515L adoptive transplant with Cxcr2KO hematopoietic donor cells demonstrated improved leukocytosis, thrombocytosis (Figure 2A) and splenomegaly in comparison to Cxcr2WT hMPLW515L recipient mice. Pathologic analysis revealed a reduction in reticulin fibrosis in bone marrow (Figure 2B) and spleen, translating into an improvement in overall survival (Figure 2C). Notably, a significant reduction in dysplastic MKs-a hallmark feature of MF-was also observed in Cxcr2KO hMPLW515L mice (Figure 2D) supporting a role for CXCR2 signaling in MK proliferation. Conclusion: IL8 secreting clones are associated with increased symptom severity and fibrosis grade in MF. Gene expression of MF CD34+ IL8 secreting clones shows up-regulation of inflammatory genes S100A8/A9, implicated in myofibroblast proliferation. Cxcr2 KO abrogates fibrosis formation and prolongs survival in the hMPLW515L model, and CXCR1/2 inhibition impairs colony forming capacity of MF CD34+ cells. These data suggest pharmacologic inhibition of this pathway should be investigated as potential therapy in MF and in PV/ET patients at high risk of fibrotic transformation. Disclosures Fan: IsoPlexis: Current Employment, Current equity holder in private company; Singleron Biotechnologies: Current Employment, Current equity holder in private company. Levine:Morphosys: Consultancy; Prelude Therapeutics: Research Funding; Qiagen: Current equity holder in publicly-traded company, Membership on an entity's Board of Directors or advisory committees; Gilead: Honoraria; Loxo: Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees; Novartis: Consultancy; Amgen: Honoraria; Astellas: Consultancy; Imago: Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees; C4 Therapeutics: Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees; Isoplexis: Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees; Celgene: Consultancy, Honoraria, Research Funding; Roche: Consultancy, Honoraria, Research Funding; Lilly: Consultancy, Honoraria; Janssen: Consultancy. Hoffman:Protagonist: Consultancy; Abbvie: Membership on an entity's Board of Directors or advisory committees; Dompe: Research Funding; Novartis: Membership on an entity's Board of Directors or advisory committees; Forbius: Consultancy.
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Swamynathan, Shivalingappa K. "Ocular Surface Development and Gene Expression." Journal of Ophthalmology 2013 (2013): 1–22. http://dx.doi.org/10.1155/2013/103947.

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The ocular surface—a continuous epithelial surface with regional specializations including the surface and glandular epithelia of the cornea, conjunctiva, and lacrimal and meibomian glands connected by the overlying tear film—plays a central role in vision. Molecular and cellular events involved in embryonic development, postnatal maturation, and maintenance of the ocular surface are precisely regulated at the level of gene expression by a well-coordinated network of transcription factors. A thorough appreciation of the biological characteristics of the ocular surface in terms of its gene expression profiles and their regulation provides us with a valuable insight into the pathophysiology of various blinding disorders that disrupt the normal development, maturation, and/or maintenance of the ocular surface. This paper summarizes the current status of our knowledge related to the ocular surface development and gene expression and the contribution of different transcription factors to this process.
10

Pope, Kevin O., Adriana C. Ocampo, Gary L. Kinsland, and Randy Smith. "Surface expression of the Chicxulub crater." Geology 24, no. 6 (1996): 527. http://dx.doi.org/10.1130/0091-7613(1996)024<0527:seotcc>2.3.co;2.

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You might also be interested in the extended bibliographies on the topic 'Expression à la surface de la levure' for particular source types:
Journal articles Dissertations / Theses Books

Dissertations / Theses on the topic "Expression à la surface de la levure":

1

Pruvost, Tiphanie. "Ingénierie moléculaire de la réactivité croisée inter-espèces d’anticorps thérapeutiques par Yeast Surface Display." Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPASQ074.

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Le succès des anticorps monoclonaux comme outils thérapeutiques s'explique en partie par leur grande spécificité pour leur cible. Celle-ci implique souvent qu'un anticorps développé contre une cible humaine échoue à la reconnaître chez les espèces utilisées lors des essais précliniques. L'objectif de ces travaux est de développer une méthode d'ingénierie de protéine permettant à un anticorps de reconnaître un antigène provenant d'espèces différentes en utilisant comme modèles deux anticorps reconnaissant la protéine LAMP1 humaine mais pas ses versions murine et simienne. Durant ce projet le principe de mutagénèse exhaustive à l'acide aminé près (DMS) est couplé à l'expression de protéines à la surface des levures (YSD) ainsi qu'à la cytométrie en flux. Une première partie détaille l'utilisation de la combinaison de ces techniques pour l'identification des épitopes des deux anticorps sur LAMP1 humain. Les acides aminés situés dans les épitopes ont alors pu être comparés à ceux des protéines murine et simienne ce qui a permis d'expliquer l'absence de reconnaissance pour ces deux protéines. La seconde partie décrit la stratégie d'ingénierie protéique développée sur ces deux anticorps. L'expression des banques de DMS à la surface des levures a permis de sélectionner des mutations améliorant la reconnaissance croisée de LAMP1 sans affecter la fonctionnalité de l'anticorps. Ces mutations ont alors été combinées dans une nouvelle banque qui a été à nouveau criblée à plusieurs reprises pour en extraire les variants les plus promoteurs. L'affinité de ces variants pour différents orthologues de LAMP1 a pu être évaluée afin de confirmer l'efficacité de la stratégie grâce à laquelle plusieurs variants cross-réactifs ont pu être obtenus pour les deux anticorps étudiés
Success of the monoclonal antibodies as therapeutic tools is partly due to their high specificity for their targets. Because of this high specificity antibodies developed against a human target often fail to recognize this target in animals used as models in preclinical trials. Thus, the goal of this study is to develop a protein engineering method aiming at conferring an antibody the ability to recognize a same antigen belonging to different species. To do so, two antibodies recognizing the human LAMP1 protein but not the murine and simian LAMP1 are used as models. In this project an exhaustive mutagenesis (DMS) monitored by Yeast Surface Display (YSD) and flow cytometry. The first part describes how these technics are combined in order to identify the epitopes of two antibodies on human LAMP1. The amino acids of epitopes are compared to those of murine and simian LAMP1 to explain the lack of recongnition of this two proteins. The second part focuses on the engineering strategy developped on the two antibodies. The expression of the DMS libraries in YSD allowed to select single mutations improving the cross-reactivity on LMAP1 without affecting the functionality of the antibodies. These mutations have been combined in a second library that has been screened for promissing variants. The affinities of these variants for differents human et simian LAMP1 orthologs has been measured to confirm the succes of the strategy. For each antibody many cross-reactive variants have been obtained
2

Sivelle, Coline. "Conception et production d’anticorps anti-TNFa non immunogènes pour le traitement des maladies inflammatoires." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS603.

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L’efficacité des anticorps anti-TNFα peut être particulièrement affectée par leur immunogénicité. Elle se traduit par la production d’anticorps dirigés contre la protéine thérapeutique (ADA) et induisant des effets indésirables. Pour l’adalimumab (Humira®), qui est l’anti-TNFα le plus utilisé, ce phénomène est observé chez plus de 30% des patients pour certaines pathologies. Les épitopes T responsables de cette immunogénicité sont principalement localisés dans les régions CDR impliquées dans l’interaction avec le TNFα. L’objectif de ce travail est de retirer les séquences épitopes T de l’anticorps sans altérer sa fonctionnalité. Afin de maitriser ce problème d’immunogénicité, ces travaux de thèse proposent d’utiliser le Yeast Surface Display (YSD) afin de contrôler la fonctionnalité de l’anticorps tout au long du processus de mutagénèse. Pour cela, une première étape de comparaison des différents formats d’expression d’anticorps en YSD a permis de définir le format d’affichage à utiliser pour la suite du projet. La stratégie de déimmunisation adoptée ensuite est basée sur la suppression des épitopes T. Dans un premier temps, elle réunit une approche de mutagénèse exhaustive (DMS) et l’utilisation d’algorithme afin d’identifier les substitutions délétères pour l’interaction HLA II/épitope T, mais neutre pour la fonction de l’anticorps. Dans un second temps, ces substitutions sont combinées dans des banques pré-enrichies en mutants fonctionnels. Des mutants ayant une immunogénicité potentielle réduite ont ensuite été sélectionnés à partir de ces banques. Plusieurs mutants de l’adalimumab à l’immunogénicité réduite selon l’algorithme de prédiction ont été identifiés et caractérisés. Ils possèdent tous une affinité augmentée pour le TNFα se traduisant par une activité au moins doublée par rapport à l’adalimumab
Efficacy of anti-TNFα antibodies is well known to be affected by their immunogenicity. Some patients developp anti-drug antibodies (ADA) which can elicit adverse effects and neutralize the therapeutic protein. Adalimumab (Humira®), which is the most used anti-TNFα, is reported to be immunogenic for more than 30% of patients in some diseases. T-cell epitopes that account for its immunogenicity are mostly carried by regions implied in the interaction with TNFα. In the present work, we propose to remove T-cell epitopes from the antibody sequence while maintaining its functionality.To undertake this issue, this PhD project uses Yeast Surface Display (YSD) to monitor the affinity of the biologic during the mutagenesis process. To do so, a comparison of different expression formats of antibody in YSD has been performed in order to define the format that will be used for the deimmunization method. Then, the strategy chosen to reduce immunogenicity is based on T-cell epitopes removal. First, it merges deep mutational scanning and in silico HLA II binding prediction to identify substitutions deleterious for HLA II/T-cell epitopes interaction while neutral for the function of the biologic. Secondly, these substitutions were combined to obtain libraries pre-enriched with functional sequences. Mutants with reduced immunogenic potential were then selected from these libraries. Several mutants of adalimumab with reduced immunogenicity potential according to HLA II binding prediction were identified and caracterized. All of them show an increased affinity for TNFα associated with an improvement of activity of at least two fold in comparison to adalimumab
3

Kudla, Bernard. "Construction d'un vecteur d'expression pour la levure Schizosaccharomyces pombe." Paris 11, 1987. http://www.theses.fr/1987PA112080.

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Le vecteur d'expression pBK02 a été construit pour la levure Schizosaccharomyces pombe à partir d'un promoteur fort détecté chez cet organisme. Le plasmide navette E. Coli/S. Pombe pUZL contenant l'opéron bactérien lactose délété de ses séquences promotrices a été utilité. Il nous a permis de mesurer chez S. Pombe, l'activité promotrice de différente fragments génomiques de cette levure insérés devant l’opéron délété. L'insertion du plasmide appelé p 54/1 produit à l’état multicopies environ 5% des protéines sous la forme bêta-galactosidase active. Cette insertion a été choisie et le promoteur qu'elle contient, caractérisé. Les séquences chromosomiques homologues au promoteur ont été clonées et le transcrit TA correspondant a été mis en évidence. L'efficacité du promoteur 54/1 à diriger la synthèse d'un transcrit hétérologue (ARN LacZ) a été comparée à celle qu'il présente à diriger la synthèse d'un transcrit homologue (ARN TA). Le promoteur 54/1 ne semble pas affecté par la séquence dont il dirige la synthèse. Ce promoteur a été utilisé pour faire exprimer chez S. Pombe différents gènes hétérologues: lacZ de E. Coli, XylE de P. Putida, Phlr de E. Coli et amy de B. Licheniformis.
4

Klonowska, Agnieszka. "Expression hétérologue de laccases du champignon Marasmius quercophilus chez la levure Saccharomyces cerevisiae." Aix-Marseille 3, 2000. http://www.theses.fr/2000AIX30085.

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Marasmius quercophilus est un basidiomycète qui colonise la litière de chêne vert (Quercus ilex L. ). Ce champignon utilise plusieurs enzymes lui permettant de dégrader la lignine afin d'accéder à la cellulose. In vitro, il a une très forte activité d'oxydation des phénols consécutive à l'expression de plusieurs formes de laccases parmi lesquelles Lac1 est majoritaire. Ces enzymes à cuivre, utilisant l'oxygène moléculaire pour oxyder de très nombreux substrats, sont au centre de projets d'applications biotechnologiques. A partir de culture en conditions contrôlées nous avons purifié et partiellement caractérisé 3 formes de laccase autres que Lac1. Quatre membres de la famille multigénique qui code pour ces enzymes ont été clonés et séquencés. Parmi ceux-ci, lac1, le gène qui code pour la forme majoritaire, a été formellement identifié. Les ADN complémentaires correspondants à ces gènes ont été exprimés dans la levure Saccharomyces cerevisiae. Deux d'entre eux, l'ADNc17 (lac1) et l'ADNc20, conduisent à la synthèse de laccases sécrétées et actives. .
The basidiomycete Marasmius quercophilus colonizes the decaying litter of the evergreen oak (Quercus ilex L. ). This mushroom uses several enzymes to degrade lignin in order to have an access to cellulose. In vitro, it has a strong phenol oxidation activity related to the expression of several laccase isoforms among which Lac1 is predominant. Using molecular oxygen to oxidise numerous substrates, these copper enzymes are at the heart of many biotechnological projects. From controled culture conditions, we purified and partially characterized three laccase isoforms other than Lac1. Four members of the multigenic family which encodes laccases were cloned and sequenced. Among these genes, lac1, the structural gene encoding the major isoform, was formaly identified. The complementary DNA corresponding to these genes were expressed in the yeast Saccharomyces cerevisiae. Two of them, cDNA17 (lac1) and cDNA20, lead to the synthesis of secreted active laccases
5

Lacour, Thierry. "Expression du cytochrome p45011 bovin dans la levure : identification d'un nouveau transporteur d'electrons." Université Louis Pasteur (Strasbourg) (1971-2008), 1997. http://www.theses.fr/1997STR13101.

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Dans le cadre d'un projet visant a reproduire la voie de biosynthese des hormones steroides dans la levure, nous avons etabli l'activite in vivo du cytochrome p45011 bovin dans les mitochondries de levure. Ces travaux mettent en evidence une nouvelle proteine de levure, yadr, capable de transferer les electrons indispensables a l'activite catalytique du cytochrome. Des analyses genetiques et biochimiques permettent de caracteriser cette proteine.
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Boutabout, Mansour. "La Rétrotranscriptase de l'élément Ty1 de la levure Saccharomyces cerevisiae : Clonage, expression, fidélité." Université Louis Pasteur (Strasbourg) (1971-2008), 2002. http://www.theses.fr/2002STR13157.

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Kribii, Rachida. "La squalène synthase d'Arabidopsis thaliana - caractérisation moléculaire et expression chez la levure Saccharomyces cerevisiae." Poitiers, 1996. http://www.theses.fr/1996POIT2390.

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La squalene synthase (sqs) est l'enzyme qui catalyse la premiere etape specifique de la voie de biosynthese des sterols en condensant deux molecules de farnesyl diphosphate en une molecule de squalene. Nous avons isole un adnc codant la sqs de la plante arabidopsis thaliana. L'adnc presente un cadre ouvert de lecture codant potentiellement une proteine de 410 acides amines qui presente des similarites significatives avec les sqs de levures et de mammiferes. Cet adnc a ete exprime, sous controle d'un promoteur fort de levure, dans une souche de saccharomyces cerevisiae depourvue de l'activite sqs et auxotrophe pour l'ergosterol. L'etude a revele que l'adnc, codant la qsq vegetale, ne permet pas la complementation de la mutation de la souche de levure. Nous avons pu observer, en revanche, que cet adnc conduit a la production d'une sqs fonctionnelle et dont l'activite est localisee au niveau des microsomes. Nous avons demontre que le squalene forme par l'action de l'enzyme vegetale, n'est cependant pas utilise comme substrat de la squalene epoxydase de levure, et explique ainsi l'absence de complementation. Un adnc codant une proteine chimerique, dont la region c-terminale de l'enzyme de plante a ete remplacee par la sequence correspondante chez la levure, permet a la levure deletee au niveau du gene de la sqs, d'acquerir une prototrophie vis a vis de l'ergosterol, suggerant ainsi que cette region de l'enzyme de levure est necessaire pour l'acheminement du squalene dans la voie de biosynthese de l'ergosterol. D'autre part, l'analyse du genome d'arabidopsis thaliana, par la technique de southern, revele la presence, chez cette plante, de deux genes codant la sqs organises en tandem. Le gene de sqs, correspondant a l'adnc que nous avons clone, est exprime dans les differents organes de la plante et principalement dans les racines
8

CORREDOR, RODRIGUEZ MAURICIO. "Diversite genetique, constitution chromosomique et expression genetique chez la levure fromagere halotolerant debaryomyces hansenii." Paris 11, 2001. http://www.theses.fr/2001PA112044.

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Dans ce travail nous avons construit des sondes specifiques a partir de fragments de rapd (amplification aleatoire d'adn polymorphe), qui nous a permis de developper une methode rapide et sensible pour l'identification de la levure d. Hansenii. La sonde f01pro a permis de typer les souches de d. Hansenii des ecosystemes fromageres. Les profils obtenus par rflp (polymorphisme de la longueur des fragments de restriction) avec la sonde f01pro discrimine les souches de d. Hansenii et releve un polymorphisme notable. L'analyse de ce polymorphisme par la methode upgma a mis en evidence une diversite genetique importante chez d. Hansenii, tout a fais, independante de l'origine geographique et de type de production fromagere. Cette heterogeneite significative au sein de d. Hansenii a ete correle avec les profils chromosomiques obtenus par pfge (electrophorese en champ pulse). L'analyse de caryotypes en southern blot a permis de confirmer le caractere repetitif des sondes specifiques telles que f01pro, le retrotransposon copia-like et une sequence telomerique hypothetique. De plus, les genes ribosomiques ont ete retrouves dans le chromosome le plus grand. Nous avons evalue les sondes specifiques en ecologie moleculaire afin d'etudier des micro-ecosystemes de populations de d. Hansenii. L'hybridation sur colonie avec la sonde f01pro a ete efficace pour identifier d. Hansenii au milieu des autres especes. La pcr in situ avec les amorces specifiques obtenues a partir de retrotransposon copia-like identifie specifiquement les cellules de d. Hansenii. Cependant, l'hybridation in situ d'une sonde specifique de ce retrotransposon n'a pas permis d'identifier d. Hansenii. Par ailleurs, nous n'avons pas trouve de sonde specifique capable d'identifier a la fois les deux varietes de d. Hansenii. Comme d'autres auteurs, nous avons conclu, en utilisant des outils moleculaires, que la variete fabriyii serait mieux classee comme l'espece d. Fabryii. Finalement, nous avons voulu etudier un des role biochimique de d. Hansenii dans les produits laitiers et le fromage. Nous avons etudie en particulier l'activite -galactosidase et les genes associes a cette activite lac4 et lac12. L'activite -galactosidase dans d. Hansenii a ete determine comme constitutive et le gene de lactose permease lac12 a ete identifie et partialement caracterise.
9

Helies-Toussaint, Cécile. "Expression des genes dans le testicule." Paris 11, 1996. http://www.theses.fr/1996PA11T012.

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Verdiere, Jacqueline. "Régulation de la synthèse de l'iso 2-cytochrome c chez la levure Saccharomyces cerevisiae." Paris 11, 1987. http://www.theses.fr/1987PA112281.

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Chez S. Cerevisiae deux iso cytochromes l'iso1 et l’iso2 cyt c sont synthétisés. Ils sont codés par deux gènes indépendants : CYC1 et CYP3 ; ils sont exprimés respectivement à des taux très différents, 95% et 5%. La possibilité de sélectionner des mutations surproductrices diso2 cyt c nous a permis de mettre en place un système génétique qui comprend d'une part, des mutations du gène de structure CYP1 de type "promotor up" agissant en cis et d'autre part, des mutations indépendantes des gènes de structure agissant en transe, tout particulièrement au locus CYP1. L'étude génétique des allèles mutés aux différents loci ainsi que les interactions entre mutations agissant en cis et celles agissant en trans, nous a permis d'élaborer un modèle de régulation de l'expression du gène de structure de l’iso2 : CYP3. L'analyse moléculaire a montré que les quatre mutations obtenues au locus CYP3 sont des macros lésions affectant la région située entre 140 et 500 paires de base en amont de la région codante. Deux d'entre elles sont des insertions à des positions différentes de transposons de type TYl, la troisième est une grande délétion affectant également l'expression d'un gène voisin, enfin la dernière est une petite délétion. CYP1 est un des loci agissant en trans. Nous avons montré que le produit de ce gène se comporte comme un activateur. La construction de souches double mutantes, associant à chaque mutation de type "promoter up" un des allèles mutés au locus CYP1 a montré que deux des mutations cis actives restent sous le contrôle du produit codé par CYP1, alors que les deux autres lui échappent en partie pour l'une et totalement pour l’autre. Un des allèles mutés au gène CYP1 a été cloné. LB séquence d'un fragment de 5,3 Kb a révélé que celui-ci code pour une protéine potentielle de 1. 483 acides aminés qui présente les caractéristiques d'une protéine ''DNA binding". L'analyse par "orthern blot" révèle la présence de deux transcrits de 4,8 et 2,2 Kb approximativement. La transcription du gène n'est sensible ni à l'oxygène, ni à l'hème, ni au substrat carboné. Nous avons montré que HAP1 identifié par Guarente comme nécessaire à l'activité de l'UAS (Upstream Activating Sequence) du gène CYC1 et CYP1 sont le même gène. Nous avons également montré que le produit " de CYPl est médiateur des effets de l'hème sur l'expression de gènes dépendants de l'oxygène.
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Books on the topic "Expression à la surface de la levure":

1

Leung, Roland. Persistence of procoagulant surface expression by activated platelets. Ottawa: National Library of Canada, 2003.

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Barbosa, Isabel Leal. The expression of surface antigens and phagocytic activity by human monocytes and alveolar macrophages. Uxbridge: Brunel University, 1989.

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Francqui Colloquium (3rd 1997 Brussels, Belgium). The trypanosome surface: Proceedings of the Third Francqui Colloquium, 26-27 May 1997, Brussels. Paris: De Boeck Université, 1999.

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1953-, Benovic Jeffrey L., ed. Regulation of G protein-coupled receptor function and expression. New York: Wiley-Liss, 2000.

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Michael, Conn P., ed. Gene expression in neural tissues. San Diego, Calif: Academic Press, 1992.

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Ali, Saima. Regulation of the cell surface expression and the function of GABA(B)R1a by GABA(B)R2. Ottawa: National Library of Canada, 2000.

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Kelleher, Mittie Elizabeth. Relationship of BE2 surface antigen expression to T-cell clonality in cutaneous T-cell lymphoma and scleroderma. [New Haven: s.n.], 1990.

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Smith, Alan L. The volcanic geology of the Mid-Arc Island of Dominica, Lesser Antilles: The surface expression of an Island-Arc batholith. Boulder, Colorado, USA: The Geological Society of America, 2013.

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Robinson, Elizabeth M. The effect of a shallow low viscosity zone on mantle flow and its expression at the surface of the earth. Woods Hole, Mass: Woods Hole Oceanographic Institution, 1987.

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Tooze, Jennifer Anne. Cell surface and molecular expression of immunoregulatory determinants on B-Cell chronic lymphocytic leukaemia cells and their putative normal lineage counterparts. Uxbridge: Brunel University, 1993.

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Book chapters on the topic "Expression à la surface de la levure":

1

Wang, Zhiguo, and Baofeng Yang. "Surface-Enhanced Raman Spectroscopy Method." In MicroRNA Expression Detection Methods, 275–80. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-04928-6_20.

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Mras, Gabriele M. "‘Surface’ as an expression of an intention." In Wollheim, Wittgenstein, and Pictorial Representation, 160–68. 1 [edition]. | New York: Routledge, 2016.: Routledge, 2016. http://dx.doi.org/10.4324/9781315640983-7.

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Schräml, Michael, D. Ambrosius, and M. Lanzendörfer. "Rapid Generation of Protein Variants and Subsequent Analysis by Surface Plasmon Resonace." In Cell-Free Protein Expression, 69–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-59337-6_9.

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Young, Mark T. "Studying Purinoceptor Cell-Surface Expression by Protein Biotinylation." In Methods in Molecular Biology, 137–46. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9717-6_9.

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Yi, Feng, Stephen F. Traynelis, and Kasper B. Hansen. "Selective Cell-Surface Expression of Triheteromeric NMDA Receptors." In Methods in Molecular Biology, 145–62. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7321-7_7.

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Heuner, Klaus, Michael Steinert, Claudia Dietrich, Gunter Fischer, Rolf Köhler, and Jörg Hacker. "Function and Expression of Legionella pneumophila Surface Factors." In Legionella, 43–48. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817985.ch8.

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Yi, Feng, Stephen F. Traynelis, and Kasper B. Hansen. "Selective Cell-Surface Expression of Triheteromeric NMDA Receptors." In Methods in Molecular Biology, 55–77. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3830-9_5.

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Jones, David T., Dagoberto Monroy, Zhongua Ji, and Stephen C. Pflugfelder. "Alterations of Ocular Surface Gene Expression in Sjögren’s Syndrome." In Lacrimal Gland, Tear Film, and Dry Eye Syndromes 2, 533–36. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5359-5_75.

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Lam, Clarissa, Mahmud Arif Pavel, Parul Kashyap, Zahra Salehi-Najafabadi, Victoria Valentino, and Yong Yu. "Detection of CXCR2 Cytokine Receptor Surface Expression Using Immunofluorescence." In Cytokine Bioassays, 193–200. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0928-5_17.

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Ujir, Hamimah, Michael Spann, and Irwandi Hipni Mohamad Hipiny. "3D Facial Expression Classification Using 3D Facial Surface Normals." In Lecture Notes in Electrical Engineering, 245–53. Singapore: Springer Singapore, 2014. http://dx.doi.org/10.1007/978-981-4585-42-2_29.

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Conference papers on the topic "Expression à la surface de la levure":

1

"Expression of Surface Molecules in Hematological Malignancies." In INTERNATIONAL CONFERENCE ON BIOLOGICAL RESEARCH AND APPLIED SCIENCE. Jinnah University for Women, 2024. http://dx.doi.org/10.37962/ibras/2024/70-73.

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Boukobza, Ram, and Ari Rappoport. "Multi-word expression identification using sentence surface features." In the 2009 Conference. Morristown, NJ, USA: Association for Computational Linguistics, 2009. http://dx.doi.org/10.3115/1699571.1699573.

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Geller, David S., Sheref E. Hassan, and Richard Gorlick. "Abstract 5104: Cell-surface receptor expression in osteosarcoma." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-5104.

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Ahn, Dae-Keon, Soon-Man Kwon, and Seok-Hee Lee. "Expression for Surface Roughness Distribution of FDM Processed Parts." In 2008 International Conference on Smart Manufacturing application (ICSMA). IEEE, 2008. http://dx.doi.org/10.1109/icsma.2008.4505572.

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Masoni, I., R. Brossier, J. L. Boelle, and J. Virieux. "Generic Gradient Expression for Robust FWI of Surface Waves." In 76th EAGE Conference and Exhibition 2014. Netherlands: EAGE Publications BV, 2014. http://dx.doi.org/10.3997/2214-4609.20141407.

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Civelekoglu, Ozgun, Ningquan Wang, Mert Boya, Tevhide Ozkaya-Ahmadov, Ruxiu Liu, and A. Fatih Sarioglu. "Quantitative Measurement of Cell Surface Expression Via Magnetophoretic Cytometry." In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). IEEE, 2019. http://dx.doi.org/10.1109/transducers.2019.8808437.

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Machiraju, S., N. Arger, B. Benn, A. Sommer, N. Bhakta, and L. Koth. "Differential Expression of Monocyte Cell Surface Proteins in Pulmonary Sarcoidosis." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a4511.

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Richman, Sarah A., Liang-Chuan Wang, Edmund K. Moon, Steven M. Albelda, and Michael C. Milone. "Abstract 2294: Reversible regulation of chimeric antigen receptor surface expression." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-2294.

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Gralnick, H. R., L. M. Magurder, K. Hansmann, M. Vail, G. Marti, R. McEver, and S. Williams. "THE SURFACE EXPRESSION OF ALPHA GRANULE PROTEINS FOLLOWING THROMBIN STIMULATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643859.

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We have studied the platelet glycoproteins (GP) GPIb and the GPIIb/IIIa and the expression of alpha granule proteins (AGP) on the platelet (P) surface following thrombin (T) stimulation. The platelets were separated from plasma proteins on a arabinogalactan gradient. The P were stimulated with purified alpha T 0.1u/108p. Either monospecific polyclonal or murine monoclonal antibodies were used to detect the P glycoprotein and AGP. The platelets were analyzed on an EPICS V Flow Cytometer. Resting P had small amounts of AGP (2-8%) present on their surface. Within 1-3 min. after T stimulation significantly increased amounts of PF4 (26%) vWf (8%) Ig (10%) and the 140 kD alpha granule membrane (70%) were present on the P surface. The peak expression of all the AGP occurred within 5 mins. The 140 kD activation protein remained stable over 3-60 mins, in contrast the PF4 and the vWf expression peaked at 5 mins. and then decreased to near baseline levels. The GPIb and GPIIb/IIIa showed different patterns after activation. The GPIb intensity and number of positive cells decreased over time, while the GPIIb/ IIIa increased in flourescent intensity and the number of positive cells. These studies indicate that T stimulation of AGP on the P surface. vWf and P4 have a transient appearance on the P surface while Ig and the 140 kD activation protein both appear to become stable components of the P plasma membrane. This technique of detecting platelet activation is a specific, sensitive, and rapid method.
10

Hashimoto, M., and D. Morooka. "Facial expression of a robot using a curved surface display." In 2005 IEEE/RSJ International Conference on Intelligent Robots and Systems. IEEE, 2005. http://dx.doi.org/10.1109/iros.2005.1545357.

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Reports on the topic "Expression à la surface de la levure":

1

Chaney, William G. Control of Metastasis-Associated Gene Expression by Cell-Surface Beta-1, 6 Branched Oligosaccharide Expression. Fort Belvoir, VA: Defense Technical Information Center, June 2002. http://dx.doi.org/10.21236/ada413058.

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Zaharieva, Emanuela, Elena Vikentieva, Rosica Andreeva, Dora Popova, and Zdravko Kamenov. CD36 Surface Expression on Monocytes in Type 2 Diabetes. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, April 2018. http://dx.doi.org/10.7546/crabs.2019.04.14.

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Chaney, William G. Control of Metastasis-Associated Gene Expression by Cell-Surface Beta-1,6 Branched Oligosaccharide Levels. Fort Belvoir, VA: Defense Technical Information Center, June 2003. http://dx.doi.org/10.21236/ada420126.

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Ching, Kathryn. Differential Control of ErbB2 Surface Expression in Breast Cancer Cells by an Alternatively Spliced Form of ERBIN. Fort Belvoir, VA: Defense Technical Information Center, May 2004. http://dx.doi.org/10.21236/ada427073.

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Peck, Jeremy W. Differential Control of ErbB2 Surface Expression in Breast Cancer Cells by an Alternatively Spliced Form of ERBIN. Fort Belvoir, VA: Defense Technical Information Center, May 2003. http://dx.doi.org/10.21236/ada416779.

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Brent L. Iverson, George Georgiou, Mohammad M. Ataai, and Richard R. Koepsel. New Strategies for Designing Inexpensive but Selective Bioadsorbants for Environmental Pollutants: Selection of specific Ligands and Their Cell Surface Expression. Office of Scientific and Technical Information (OSTI), February 2001. http://dx.doi.org/10.2172/789805.

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Iverson, B. L., G. Georgiou, M. M. Ataai, and R. R. Koepsel. New strategies for designing inexpensive but selective bioadsorbants for environmental pollutants: Selection of specific ligands and their cell surface expression. 1998 annual progress report. Office of Scientific and Technical Information (OSTI), June 1998. http://dx.doi.org/10.2172/13502.

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Iverson, B. New strategies for designing inexpensive but selective bioadsorbants for environmental pollutants: Selection of specific ligands and their cell surface expression. Technical progress report, September 15, 1996--September 14, 1997. Office of Scientific and Technical Information (OSTI), January 1997. http://dx.doi.org/10.2172/13501.

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9

Neodo, Anna, Fiona Augsburger, Jan Waskowski, Joerg C. Schefold, and Thibaud Spinetti. Monocytic HLA-DR expression and clinical outcomes in adult ICU patients with sepsis – a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2022. http://dx.doi.org/10.37766/inplasy2022.11.0119.

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Review question / Objective: The scope of this review was defined using PICOTS framework where 1) population: adult critically ill patients with sepsis or septic shock; 2) index prognostic factor: cell surface protein expression of mHLA-DR in blood; 3) comparative factor: none; 4) outcomes to be predicted: mortality, secondary infections, length of stay, and organ dysfunction score (sequential organ failure assessment [SOFA], multiple organ dysfunction score [MODS], logistic organ dysfunction score [LODS]), composite outcomes where component endpoints consist of at least one of the outcomes stated above (e.g., “adverse outcome” defined as death or secondary infection), 5) timing (of the prediction horizon and the moment of prognosis): any; and 6) setting: ICU. Condition being studied: Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to severe infections. It can further progress to septic shock, which includes hemodynamic failure and increased mortality rates. A recent worldwide epidemiological study estimated 48.9 million sepsis cases and 11 million of sepsis-related deaths (~20% of global deaths in 2017). Although its management has advanced considerably, sepsis remains deadly and challenging to treat. The 28/30-day mortality averages around 25% for sepsis and 38% for septic shock in high-income countries. Current models describe the underlying pathophysiologic mechanisms of sepsis as an interplay between concurrent dysfunctional pro- and anti-inflammatory immune response.
10

Cruikshank, K. M., A. M. Johnson, R. W. Fleming, and R. L. Jones. Winnetka deformation zone: Surface expression of coactive slip on a blind fault during the Northridge earthquake sequence, California. Evidence that coactive faulting occurred in the Canoga Park, Winnetka, and Northridge areas during the 17 January 1994, Northridge, California earthquake. Office of Scientific and Technical Information (OSTI), December 1996. http://dx.doi.org/10.2172/677055.

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