Dissertations / Theses on the topic 'Explant cultures'
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Teixeira, Marta Carolina Jesus. "Patient-derived explant cultures for cancer modelling." Master's thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Quimica e Biológica António Xavier, 2018. http://hdl.handle.net/10362/130073.
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Ahmed, Najma Ayesha. "Studies on the regulation of chorionic gonadotropin production in explant cultures of human placenta." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74342.
Full textPlacental hCG production was assessed using two different methods: (a) hCG concentrations in media recovered from cultures were measured by radioimmunoassay and (b) tissue levels of hCG in cultured placentae were determined immunohistochemically; both were evaluated before and after exposure to steroid hormones. In first trimester placentae, progesterone and dehydroepiandrosterone (DHEA) increased hCG concentrations both in collected medium and levels in cultured placentae. Estradiol increased the levels of hCG in tissues but not in media. Cortisol increased concentrations in media but did not alter tissue levels. Testosterone decreased hCG levels in media, but had no effect on hCG placental content. In third trimester cultures, progesterone and DHEA were the only hormones studied which increased concentrations of hCG in media; estradiol, cortisol and testosterone had no effect. Progesterone, estradiol and DHEA, alone or in combination, extended the viability of first trimester placental explant cultures from approximately 7 to 30 days. There was a significant relationship between placental viability and tissue hCG levels (r = 0.73, P $<$ 0.001). The concentrations of hCG, progesterone and estradiol in human placentae were determined at various times through gestation. These studies suggest that a temporal relationship exists between the placental levels of hCG and these steroids, and that they may be significant determinants of growth and differentiation of the placenta in vivo. Furthermore, these investigations support the hypothesis that hCG production by the placenta is subject to paracrine regulation by steroid hormones.
Baydoun, Martha. "Développement d’un modèle de culture tridimensionnelle à partir d’explants entériques pour l’étude de la cryptosporidiose." Thesis, Lille, 2018. http://www.theses.fr/2018LIL1I003.
Full textAlmost 20% of cancers are due to a viral, bacterial or parasitic infection. For instance, Cryptosporidium parvum (C. parvum) was found to induce the development of an invasive digestive adenocarcinomas in an experimental model of SCID mice.However, the understanding of the pathogenesis of Cryptosporidium has been limited by the lack of a long-term culture system. It is therefore essential to improve the in vitro culture systems by trying to mimic the in vivo conditions. Firstly, the association between Cryptosporidium infection and cancer development was investigated through an epidemiological study among cohorts of Lebanese patients with or without recent diagnosis of digestive cancer before any treatment. A high rate of Cryptosporidium was detected in biopsies from Lebanese patients with digestive neoplasia/adenocarcinoma. These results showed that Cryptosporidium is associated with human colon cancer being maybe a potential etiological agent of this disease. In the second part, a three dimensional (3D) Cryptosporidium culture model was developed from adult murine colon. This system allowed the reproduction of neoplasic lesions at 27 days post-infection, providing new evidence of the role of the parasite in the induction of carcinogenesis. This is the first description of a low-grade intraepithelial neoplasia induction after parasite infection in a 3D culture. Finally, in order to automatize the culture, a microfluidics device for explant culture was designed. An explant culture was maintained for almost 8 days using this microfluidic device. To our knowledge, this is the first description of a gut tissue culture on a microfluidic device that was viable for 8 days
Gerfin-Moser, Andrea Michaela. "Excitatory amino acid receptor expression : localisation in pigeon brain and modulation in rat hippocampal explant cultures by epileptiform activity /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10918.
Full textFougeray, Sylvie. "Toxicologie cellulaire de liquides de dialyse péritonéale sur des cultures de cellules mésothéliales de premier explant provenant de sujets IRC (insufisants rénaux chroniques) et IRC." Montpellier 1, 1996. http://www.theses.fr/1996MON13514.
Full text許霖慶 and Lam-hing Hui. "Studies on explant regeneration and morphogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1985. http://hub.hku.hk/bib/B3120692X.
Full textHui, Lam-hing. "Studies on explant regeneration and morphogenesis /." [Hong Kong : University of Hong Kong], 1985. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1231481X.
Full textPenrod, Leah Vee. "The Effects of EPA and DHA on the Uterine Inflammatory Response in Mares during In Vitro Culture of Endometrial Tissue." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/145149.
Full textJosé, Andrea Alessandra Filomena Brasil Vieira. "Efeito do ácido linoléico conjugado trans-10, cis-12 na regulação da lipogênese e expressão gênica em culturas de tecido adiposo de suínos em crescimento." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-08022006-143005/.
Full textThe objective of this study was to evaluate the effects of conjugated linoleic acid (CLA), specifically the tnas-10, cis-12 isomer on cultures of adipose tissue explants from growing pigs.
Amaral, Antonio Francisco de Campos. "Comportamento in vitro de explantes de matrizes de cenoura (Daucus carota L.) tratadas com variáveis níveis de potássio." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-14082003-165946/.
Full textThe growth of plants, organs, tissues and cells in vitro culture depends on the development of optimized culture medium for the perfect interaction among essential components such as phytoregulators, carbon source and minerals nutrients. The factors limiting the growth of organs or tissues in vitro conditions are similar to those limiting growth in vivo conditions. The objective of this work was aimed at studying the influence of the potassium nutritional status of matrixes plants of carrot Daucus carota Link on the in vitro morphogenesis. Matrixes plants were obtained from seeds germinated in organic substratum and cultivated in plastic pots containing washed sand in greenhouse conditions. The matrixes plants were then submitted to treatments with nutrients solutions containing variable potassium levels. After 30 and 60 days treatment, explants (internodes) were collected, disinfested and inoculated in solid culture medium of Murashige and Skoog (MS) containing different potassium concentrations and supplemented with 0,1mg.L -1 of 2,4-D for callogenesis induction in dark conditions. Cell differentiation by somatic embryogenesis was pursued by culturing the calli in auxina-free same culture medium in growth room under photoperiod of 16/8 hours (light/dark). The evaluation of the callogenesis induction and cell growth was carried out 60 days after explants inoculation, based on the mass of fresh and dry matter accumulation on each explant. The evaluation of cell differentiation (plant formed/explant) and of cell differentiation rate (number of plants formed/g of dry matter of callus) was carried after 30 days of culturing under light conditions. Callogenesis induction and cell growth on the explants of treated matrixes plants were affected by the potassium treatment levels in the nutrient solution and by the duration of the treatments. Explants from treated plants with the higher K + concentrations showed callus induction and growth inversely proportional to the concentration of K + in the culture medium for both (30 and 60 days) treatment duration. However the callogenesis accumulated after 60 days treatment was twice as much as that of 30 days treatments. Short time treatments duration (30 days) with higher levels of K + in the nutrient solutions and low concentrations of K + in the culture medium influenced the cell differentiation negatively (nº plants/explant) in the callus of the explants from treated plants. Cells from calli induced on explants from matrixes plants for 30 days were more morphogenic than the cells in the 60 days treatment where high callogenesis was observed. Also better cell differentiation rate was observed on calli induced on explants from treated matrixes plants with nutrient solutions containing the highest potassium levels and inoculated on MS culture medium containing highest potassium concentrations.
Mount, Seth. "Serum-Free Xenogen-Free Culture Conditions Support Human Explant-Derived Cardiac Stem Cell Growth." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35678.
Full textVan, Lerberghe Nadine. "Phénotypes comparés des fibroblastes dermiques de souris vieillissant dans l'animal et dans différentes conditions de culture: explants, cultures sur substrats plan, cultures en gels de collagène. (Immunocytochimie photonique et microscopie électronique)." Doctoral thesis, Universite Libre de Bruxelles, 1986. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213522.
Full textMurray, Colin Alexander. "Periradicular disease (PRD) : development of a novel explant culture model to investigate the cytokine network." Thesis, University of Glasgow, 2005. http://theses.gla.ac.uk/5370/.
Full textReinsberger, Claus. "Do cultured human skin explants elaborate coeliac antigen, possibly even Tissue-Transglutaminase?" [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966537475.
Full textHoepker, Veit Helmut. "Trophic actions of myenteric plexus explants on striatal neurons in cell culture." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283397.
Full textWetten, Andrew C. "The influence of light regime on growth and differentiation of cultured tomato explants." Thesis, Oxford Brookes University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315398.
Full textOmwenga, George Isanda. "Callus Development and Organogenesis in Cultured Explants of Cowpea (Vigna unguiculata (L.) Walp." Thesis, University of North Texas, 2004. https://digital.library.unt.edu/ark:/67531/metadc4655/.
Full textDavies, Catrin Meleri. "Validation of an ex vivo, loaded, circumfusion culture for living cancellous bone explants." Thesis, Cardiff University, 2005. http://orca.cf.ac.uk/54559/.
Full textMorin, Caroline. "Caractérisation des propriétés mécaniques et électrophysiologiques des explants bronchiques mis en culture organoïde." Mémoire, Université de Sherbrooke, 2005. http://savoirs.usherbrooke.ca/handle/11143/3812.
Full textMorin, Caroline. "Caractérisation des propriétés mécaniques et électrophysiologiques des explants bronchiques mis en culture organoïde." [S.l. : s.n.], 2005.
Find full textWilliams, Adam. "Proteomic studies of an explant model of equine articular cartilage in response to pro-inflammatory and anti-inflammatory stimuli." Thesis, University of Nottingham, 2014. http://eprints.nottingham.ac.uk/14371/.
Full textJesty, James Henry Frederick. "The behaviour of coconut and wheat leaf explants in tissue culture : a comparative study." Thesis, Cardiff University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305476.
Full textNikolakis, Georgios [Verfasser]. "Towards the establishment and characterization of a human skin explant co-culture model with SZ95 sebocytes / Georgios Nikolakis." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2014. http://d-nb.info/1056908041/34.
Full textMatsunaga, Mami. "Initiation of Supporting Cell Activation for Hair Cell Regeneration in the Avian Auditory Epithelium: An Explant Culture Model." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263555.
Full textDavies, Sallie. "IGFBP-1 production in an explant culture system of human Fallopian tube mucosal cells and influences upon that production." Thesis, University of Southampton, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.427456.
Full textKarekla, Ellie. "Improving therapeutic approaches for the treatment of non-small cell lung cancer using an ex-vivo explant culture system." Thesis, University of Leicester, 2015. http://hdl.handle.net/2381/39930.
Full textJones, William Dylan. "Epidermal adhesion molecules in human wounds and development of a tissue explant culture system to investigate modification of their expression." Thesis, Cardiff University, 2004. http://orca.cf.ac.uk/55575/.
Full textOliveira, Leandro Silva de. "Propagação de Eucalyptus cloeziana F. Muell." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11150/tde-05052014-154718/.
Full textEucalyptus cloeziana has importance for its wood technologic characteristics, mainly the durability, density and resistance. Moreover, this species has limitations on adventitious rooting of cuttings, having difficulties to obtain clonal seedlings and to advance in the improvement programs. In this perspective, the present work aimed to evaluate the E. cloeziana micropropagation by juvenile and mature explants as a technique for propagation this specie. Therefore, the work was divided into four basic studies. The first study was the establishment of the protocol to indirect organogenesis to E. cloeziana hypocotyls and cotyledons. In the second study evaluated the rescue of E. cloeziana adult matrices for epicormic shoots induction in crow branches. In the third study was definite a micropropagation protocol by axillary branching of E. cloeziana adult matrices. Finally, in the fourth study was evaluated the in vitro rejuvenation of E. cloeziana adult matrices to micropropagation by axillary branching and the micro-cutting technique of in vitro rejuvenated matrices to prove the in vitro culture viability to the propagation of this specie. The indirect organogenesis E. cloeziana was dependent of explant type and growth regulator and its concentration used in the different morphogenesis phases. The adventitious shoots were multiplicities and acclimatized with success to obtain E. cloeziana clonal seedlings. The vegetative rescue results of E. cloeziana adult matrices showed a higher induction epicormic shoots from branches collected in warmer and rain season. The medium diameter between 2.0 and 5.0 cm was considered the better to obtain higher epicormic shoots number in vegetative rescue. The micropropagation protocol of E. cloeziana adult matrices was realized with success using epicormic shoots, induced in the branches, like explants. The shoots in vitro multiplication was realized on WPM medium culture, supplemented with BA and NAA. The better growth regulators concentrations were different for each genotype. Treatments pulse with GA3 was not suitable to promote the elongation of shoots in vitro, which was obtained by the BA concentration reduction at 0.1 mg L-1. The in vitro shoots acclimatization and ex vitro rooting was performed in mini-incubators with success and it permit to obtain clonal seedlings from E. cloeziana adult matrices to form a clonal micro-garden. The shoots of E. cloeziana adult matrices showed different in vitro multiplication rates with specific-genotype responses to micropropagation. The micro-cutting technique results of E. cloeziana adult matrices provide evidence that occurred in vitro rejuvenation of these genotypes, during the micropropagation. Therefore, the nursery results showed that other factors, not only maturation, can be involved in the adventitious recalcitrance of E. cloeziana. In conclusion, the importance micropropagation like a biotechnological tool to propagation of E. cloeziana was proved and opened important perspectives for future investigations to optimize the propagation methods for this specie.
Polesi, Natalia Pimentel Esposito. "Estudo da comunidade bacteriana endofítica e de sua manifestação na micropropagação de Eucalyptus benthamii." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-22092015-143023/.
Full textEucalyptus benthamii has proven to be especially advantageous as an alternative culture in cold regions, justifying efforts to establish protocols for micropropagation. However, the matrices are preferably selected when adults (material with lower morphogenic efficiency), making micropropagation more dependent of subcultures and too longer to reverse the material to rejuvenation. Thus, reduction of losses in vitro has deserved attention, such for example the endophytic manifestations that require the maximization of efficiency culture and adjustments to the Protocol, in order to minimize them, enabling better understanding of the relations established and maintained between endophytes and its host along micropropagation. For this, mini-stumps were used from two sources of mini-cuttings collected from the epicormic shoots of mega-cuttings from the treetop base and shoots from girdling from the trunk base, both of one E. benthamii matrix with 13 years of age established in clonal mini garden under greenhouse condition, aimed to evaluate how is the multiplication of two sources of explants (mini-stumps) under different growing conditions; analyze how the endophytic manifestations frequency and intensity are affected by different conditions; investigate the changes to occurrence in the endophytic bacterial community due to the variation of culture conditions and micropropagation phase (in vivo = mini-stumps, and in vitro = micro-stumps, elongated and rooted materials). In order to meet these objectives, the research was divided in two parts. In the first (chapter 3) the development, morphophysiological aspects, histochemical and endophytic manifestation were evaluated in the multiplication of the two explants sources from different media and culture conditions. In the second (chapter 4) endophytic bacterial communities were analyzed by PCR-DGGE based on the V6 region of 16S rDNA gene. The results showed that micro-stumps from mega-cuttings had better development regardless of treatment and increased frequency of endophytic events, comparing with the girdling. Endophytic bacterial communities were different between samples in vivo and in vitro, and have changed over the subcultures and the elongated and rooted samples. The differences in the development of micro-stumps can be explained by the totipotentiality inherent to the material, but may also be affected by both the manifestation occurrence and the endophytic bacterial community more or less sensitive to the micropropagation, helping or harming the in vitro development of their hosts. We also highlight that even in an aseptic and environmentally controlled system, the endophytic microorganisms that resisted the whole process of disinfection and cultivation, are not \"asleep\", quite the opposite may change in quantity when your host is subjected to a new cultivation system (in vitro establishment) and a new phase within the micropropagation (multiplication → stretching and enraizamnento), or even along the subcultures. This way, the complex network of relationships of the plant with their endophyte does not cease during the in vitro culture, unlike remains dynamic.
Hirota, Keisho. "Live imaging analysis of the growth plate in a murine long bone explanted culture system." Kyoto University, 2019. http://hdl.handle.net/2433/242405.
Full textHussein, Salem Hussein. "Aspects of embryogenesis and organogenesis in the in vitro culture of various explants of the eggplant (Solanum melongena L.)." Thesis, University of Southampton, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316471.
Full textAttfield, Elizabeth Margaret. "An analysis of the stages of organogenesis in leaf explants of Nicotiana tabacum L. CV. Xanthi NC. cultured in vitro." Thesis, University of Southampton, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303344.
Full textWang, Shiping. "Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells." Diss., Virginia Tech, 1994. http://hdl.handle.net/10919/39137.
Full textLiu, Hongxiang. "Effects of pulsed electromagnetic fields on extracellular matrix composition of embryonic chick sternal cartilage explanted to culture." Thesis, Royal Veterinary College (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.362746.
Full textFávero, Pâmela. "Transformação genética de três cultivares de laranja doce a partir de explantes de plantas adultas." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-11022011-094748/.
Full textThe difficulty of tissue transformation of adult woody species is still an obstacle to be overcome for most sweet orange cultivars in the Brazilian citrus industry. This difficulty is related to the high level of contamination, low regenerative potential and low transformation capacity of adult tissues of woody species. Thus, the objective of this work was to optimize the protocol for genetic transformation of adult tissue of three cultivars of sweet orange. Therefore, with in vitro culture adjustments, it was possible to obtain transgenic adventitious buds though Agrobacterium tumefaciens of three sweet orange cultivars (Citrus sinensis L. Osbeck), Hamlin, Pêra and Valencia, using adult material as explants source. The transgenic buds were identified as transgenic by the GUS histochemical test and confirmed by the PCR analysis, which showed fragment amplification of 817 bp corresponding to the part of the amplified uidA gene. The use of co-culture media rich in auxins and cytokinins (2.0 mg L-1 of 2.4 D, 2.0 mg L-1 of AIA and 1.0 mg L-1 of 2-iP) was more efficient for all three cultivars. Selection media with high concentrations of cytokinin (3 mg L-1) promoted the regeneration and, consequently, the transformation of adventitious buds in the three cultivars. The use of sonication was not effective to reduce endophytic contamination. Moreover, this procedure reduced transformation efficiency of explants. The average efficiencies of genetic transformation for the three varieties were 2.5% for Hamlin, 1.4% for Pêra and 3.7% for Valência.
León, Enrique Asterio Benítez. "GERMINAÇÃO, ESTABELECIMENTO, REGENERAÇÃO E CALOGÊNESE IN VITRO EM EXPLANTES DE AÇOITA-CAVALO (Luehea divaricata Mart. & Zucc.)." Universidade Federal de Santa Maria, 2010. http://repositorio.ufsm.br/handle/1/8668.
Full textThe advance of biotechnology in forestry has favored techniques of in vitro propagation of species that present difficulties in reproduction by sexual seeds. Luehea divaricata Mart. & Zucc, a member of the Malvaceae family, is a species that has suffered in recent decades, a large reduction in the areas occupied by natural populations, because of its use in the manufacture of furniture. Additionally, it presents slow and irregular germination and seed viability is very uneven, characteristics that contribute to a reduced ability to recover natural populations. Considering the potential for the application of techniques of tissue culture in the mass propagation of this species, this study aimed to: develop a methodology to promote an efficient surface disinfestation of seed germination in order to in vitro evaluate the ability of two types of explants of seminal in performing the in vitro establishment; evaluate the nodal segments isolated from of plants grown in vitro germination depending on the addition of BAP and NAA in different concentrations, in the WPM nutrient medium, and, finally, analyze the calli seetings responses of leave fragments to different concentrations of BAP and NAA in WPM. The project was developed at the Laboratory of Tissue Culture at the Center for Biotechnology and Breeding of the Federal University of Santa Maria. The seeds used were collected by Fepagro/Forests and the plants obtained were used as source of explants for the experiments in tissue culture. We tested the effect of different concentrations and immersion times of seeds in mercuric chloride, and also the influence of fungicides Dicarboximida and Benzimidazole in the surface disinfestations. To in vitro establish were evaluated apical and nodal segments isolated from plants after 60 days in vitro culture. The surface disinfestation of L. divaricata seeds with mercuric chloride was efficient, promoting a satisfactory germination and lack of phytotoxicity. Moreover, the use of fungicides, after disinfestation procedure with mercuric chloride, showed toxic effect on plants, failed to control contamination by microorganisms. The L. divaricata in vitro establishment was successful from both the apical and nodal segments, especially those that result in higher leaf development. Concentrations of BAP and NAA tested dont not result in a hormonal balance appropriate to promote optimal responses in vitro multiplication of L. divaricata from the culture of nodes segments and the presence of BAP influence significantly the calli induction, unlike NAA which dont not affect the calli formation on leaf fragments. Further studies should be performed to optimize the in vitro surface desinfestation, multiplication and callus induction capable of producing a larger number of aseptic cultures to continue to other steps in tissue culture. Finally, studies that aim to contribute to the L. divaricata micropropagation should continue, since the species presents barriers to the spread by seeds that can be overcome by tissue culture.
O avanço da biotecnologia na área florestal tem favorecido as técnicas de propagação in vitro de espécies que apresentam dificuldades na reprodução via sementes. Luehea divaricata Mart. & Zucc (açoita-cavalo), integrante da família Malvaceae, é uma espécie florestal que sofreu, nas últimas décadas, grande redução das áreas ocupadas por populações naturais, em função do interesse de seu uso na fabricação de móveis vergados. Adicionalmente, apresenta germinação lenta e irregular e a viabilidade das sementes é muito desuniforme, características que contribuem para uma reduzida capacidade de recuperação natural das populações. Considerando-se a potencialidade da aplicação de técnicas de cultura de tecidos na propagação massal dessa espécie, o presente trabalho teve como objetivos: desenvolver uma metodologia que promova uma eficiente desinfestação superficial de sementes, visando à germinação in vitro; avaliar a capacidade de dois tipos de explantes de origem seminal em realizar o estabelecimento in vitro; avaliar a regeneração de segmentos uninodais isolados de plantas oriundas da germinação in vitro em função da adição da citocinina BAP e da auxina ANA, em diferentes concentrações, ao meio nutritivo WPM; e, por fim, analisar as respostas calogênicas de fragmentos foliares, face às diferentes concentrações de BAP e ANA em meio nutritivo WPM. Os trabalhos foram desenvolvidos no Laboratório de Cultura de Tecidos, do Núcleo de Biotecnologia e Melhoramento do da Universidade Federal de Santa Maria. Foram utilizadas sementes coletadas pela Fepagro/Florestas e, as plantas obtidas, foram utilizadas como fonte de explantes para os experimentos de cultura de tecidos. Foram testados o efeito de diferentes concentrações e de tempos de imersão das sementes em bicloreto de mercúrio, e, também, a influência de fungicidas Dicarboximida e Benzimidazol na desinfestação superficial. Para o estabelecimento in vitro, foram avaliados segmentos nodais e apicais caulinares isolados de plantas, após 60 dias de cultivo in vitro. A desinfestação superficial das sementes de açoitacavalo com bicloreto de mercúrio foi eficiente, promovendo uma germinação satisfatória e ausência de fitotoxicidade. Por outro lado, o uso de fungicidas, após procedimento de desinfestação com bicloreto de mercúrio, além de apresentar efeito tóxico para as plantas, não controlaram a contaminação por microrganismos. O estabelecimento in vitro de açoitacavalo foi efetuado com sucesso, tanto a partir de segmentos apicais, como segmentos nodais, com destaque para estes pelo maior desenvolvimento foliar. As concentrações de BAP e ANA testadas, não resultam em um balanço hormonal adequado à promoção de respostas otimizadas na multiplicação in vitro de açoita-cavalo, a partir do cultivo de segmentos nodais; a presença de BAP influencia, significativamente, a calogênese, ao contrário de ANA, que não afeta a formação de calos em fragmentos foliares de açoitacavalo. Estudos adicionais devem ser realizados no sentido de otimizar a desinfestação superficial, a multiplicação e a calogênese in vitro, permitindo a obtenção de um número maior de culturas assépticas para dar continuidade a outras etapas da cultura de tecidos. Finalmente, os estudos que visam contribuir para a micropropagação em açoita-cavalo devem prosseguir, já que a espécie apresenta entraves para a propagação por sementes que podem ser superados pela cultura de tecidos.
Silva, Herbert Gomes da. "O explicar: a explicação humana na perspectiva do observador como ser biológico e cultural." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/81/81133/tde-25022013-133523/.
Full textIn this research we study and defend the phenomenon of the explain from the observer\'s perspective. The conceptual system that emphasizes the observer as a biological and cultural being based on the Theory of the Biology of Knowledge by Humberto Maturana and Francisco Varela. The explanation is a human phenomenon and simultaneously biological that only exists when is accepted by the observer. The interaction is possible when the biological constitution of the observer allows an interference of the external environment to his internal dynamics, directly related to the nervous system that operates on operational clausura. The observer is an autopoietic being, therewith suffers constant changes in his structure in the interactional processes of the praxis of the living, and yet, no losing his identity. When there is more than one observer that interacts and modifies each other, besides being in a structural coupling, they begin to live and have a set of behaviors that depend on their collective history (ontogeny). To illustrate models of accepted explanations has been collected statements with students of the 9th elementary grade of a public school in Sao Paulo. From that statements, it was possible to create categories that demonstrated the existence of elements in the students\' explanations from the context of the praxis of the living, which would indicate that the education of science should reflect on it as a key element to reference the language and teaching methods, making science as something natural to make its observers who seek to know what is on scientists doings, because \"All doings is a knowledge, and all knowledge are doings\". The autopoietic observer is in an indissociable relationship between language, biological constitution and culture.
Faria, Daniele Vidal. "Otimização do protocolo de organogênese in vitro em explantes hipocotiledonares de urucum (Bixa orellana L.)." Universidade Federal de Viçosa, 2014. http://www.locus.ufv.br/handle/123456789/7577.
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Com a substituição dos corantes sintéticos pelos naturais, o urucum (Bixa orellana L.) tem alcançado crescente importância no mercado internacional, por ser a principal fonte do apocarotenoide bixina, utilizada para vários fins na indústria, sendo o único responsável por suprir a demanda do comércio mundial. Neste cenário busca-se a compreensão da via e a clonagem dos genes envolvidos na via de biossíntese da bixina, visando principalmente à obtenção de plantas melhoradas, bem como para inserção de genes da própria via em outras espécies comerciais de cultivo amplo e mecanizado. Atualmente trabalhos relacionados à transformação genética em B. orellana são escassos e há a necessidade de otimizar métodos de regeneração in vitro que viabilizem a obtenção de plantas transgênicas com desempenho superior em nível de produção de bixina. Dessa forma o objetivo desse trabalho foi testar diferentes explantes hipocotiledonares de urucum, citocininas e diferentes meios de cultura na organogênese, desenvolvimento e enraizamento de brotos de B. orellana. Foi observado que explantes hipocotiledonares contendo tamanho de 6,0; 8,0; e 10,0 mm de comprimento apresentaram eficientes respostas organogênicas, sendo observada a formação de até 10 brotos por explante quando cultivados no meio JADS suplementado com zeatina. Em explantes hipocotiledonares de urucum seccionado longitudinalmente e posicionados com a secção em contato com o meio de cultura JADS acrescido de zeatina foi observado a formação de 17,11 brotos por explante. Explantes hipocotiledonares pré-tratafdoscom Zeatina, BAP e TDZ (0,1 e 0,5 mg L-1) não diferiram significativamente na indução de brotos em comparação com explantes não tratados com citocininas. O meio de cultura JADS se mostrou mais eficiente em comparação ao MS, induzindo maior número de brotos. Os brotos apresentaram-se mais alongados, vigorosos e totalmente não hiperhídricos. Os brotos induzidos em meio JADS apresentaram também maior número e maior desenvolvimento de raízes em comparação com brotos induzidos em meio MS de urucum, consequentemente, devido ao desenvolvimento superior, brotos induzidos no meio JADS, apresentaram 100% de sobrevivência durante a fase de aclimatização ex vitro. O presente trabalho possibilitou otimizar as respostas organogênicas em diferentes explantes hipocotiledonares de urucum, concentrações de fitorreguladores e meios de cultura, os quais podem ser utilizados para estudos futuros de transformação genética de urucum.
With the substitution of synthetic dyes to natural, annatto seeds (Bixa orellana L.) industry is increasing the international market importance, given that this species is the main natural and commercial source of the apocarotenoid bixin. This plant pigment is used for various industrial purposes, and it is the main pigment that has been comerciallized worldwide. In this scenario, it is necessary to understand the metabolic pathway and the cloning of the genes involved in the bixin biosynthesis, aiming to obtain improve plants or even to add genes of interest related to bixin biosynthesis to other commercial crop species of broad and mechanized cultivation. Currently, there are few publications related to genetic transformation in B. orellana, being necessary to optimize in vitro protocols that will allow the obtainining of transgenic plants with higher level of bixin production. Thus, the aim of this work was to test different hypocotyl explants of annatto, the cytokinin effect and different media on in vitro organogenesis and rooting of B. orellana adventitious shoots. It was observed that hypocotyl explants with 6.0, 8.0 and 10 mm longer showed the best organogenic responses inducing up to 10 shoots per explant when cultured in JADS medium supplemented with zeatin. Hypocotyl explants of annatto induced more shoots when they were sectioned longitudinally and positioned with the section in contact with the JADS medium in presence of zeatin, which stimulated the induction of 17.11 shoots per explant. The hypocotyl explants pre-treated with zeatin, BAP and TDZ (0.1 and 0.5 mg L- ) did not differ significantly on shoot induction from control explants which were not treated with cytokinins. The JADS medium was more appropriate for shoot induction in comparison with MS medium. The shoots induced on JADS medium were more vigourous, longer and non-hiperhidric. The JADS medium affect positively the rooting, given that the shoots displayed more roots and well developed roots in comparison with shoots induced on MS medium. Consequently, due to the best development on JADS medium, the shoots supported the ex vitro acclimatization phase with 100% of survival. This work possibilited the optimization of organogenesis from different hypocotyl explants, plant growth regulator concentrations and culture media, which can be used in further studies of annatto genetic transformation.
Vieta, i. Corcoy Pep Anton. "Nous formats en la comunicació de la química. Explorant el seu paper en el foment de la cultura i les vocacions científiques." Doctoral thesis, Universitat de Girona, 2017. http://hdl.handle.net/10803/404101.
Full textAl llarg d’aquesta tesi s’han dut a terme projectes de comunicació científica on la innovació i la recerca han estat aplicades per millorar les propostes existents, explorar altres formats i, tot experimentant, proposar novetats sense deixar de banda l’avaluació de la repercussió de l’activitat divulgativa sobre el públic, en el foment de vocacions i en la millora de la percepció social de la ciència. Molts dels canvis socials ocorreguts durant el transcurs de la tesi han estat presos com a reptes de la mateixa esdevenint oportunitats ben aprofitades. Entre ells destaquen les xarxes socials, com a noves eines de comunicació digital, o la popularització del coneixement obert, així com els MOOC o la flipped classroom, com a metodologies innovadores en l’àmbit de l’educació
Mosquim, Paulo Roberto. "Cultura in vitro de "Explants" de soja (Glycine max CLJ Merril) desenvolvimento da tecnica e avaliação de diferentes fontes de nitrogenio." [s.n.], 1989. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315230.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: No presente trabalho desenvolveu-se um sistema de ¿explant¿ que possibilitou verificar o transporte e o metabolismo de substâncias nitrogenadas, em caules, vagens, tegumentos das sementes e cotilédones das sementes de soja (Glycine Max [L] Merril) cv. Santa Rosa. O ¿explant¿ utilizado continha apenas uma vagem completatmente expandida com duas sementes (pesando em torno de 200 mg) um caule com cerca de 6 cm de comprimento e um pedaço de pecíolo remanescente. A base do caule era então mergulhada em frasco contendo um meio de cultura definido, pH 5.0, mantido a 2ºC, em banho de gelo. As vagens permaneceram a uma temperatura de 25ºC, sob luz contínua em uma câmara de germinação. Nos testes para verificar as melhores condições do meio de cultivo que assegurassem melhores crescimentos das sementes verificou-se que: Não houve necessidade da adição de cofatores ou vitaminas. A melhor composição de macronutrientes do meio foi a de CHANDLER et al (1983). As melhores concentrações de sacarose estão entre 33,3 e 66,6 mg/ml do meio e a de glutamina foi de 6,26 mg/ml. Os caules apresentaram pouca reserva de carboidratos, mas continham reservas de substâncias nitrogenadas. Não houve necessidade da substituição dos meios de cultivo durante o período de análise. ...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital
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Doutorado
Doutor em Ciências Biológicas
Patel, Taranjeet. "Using dynamic cultural theories to explain the viability of international strategic alliances : a focus on Indo-French alliances." Thesis, Open University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422015.
Full textCapaldi, Flávia Regina. "Avaliação de diferentes fontes de nitrogênio em explantes de Cryptomeria japonica D.Don. "elegans" cultivados in vitro: análises bioquímicas e relações entre reguladores vegetais." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/11/11150/tde-30072002-162030/.
Full textCryptomeria japonica D. Don. "elegans" is a fast-growing tree belonging to the Taxodiaceae and it is considered a responsive species 'in vitro'. The aim of this work was to evaluate the effect of ten different concentrations of nitrate and ammonium in morphogenesis 'in vitro' of Cryptomeria japonica D. Don. "elegans" explants. Analysis of relative growth rate, total soluble proteins, total soluble aminoacids, total non structural carbohydrates and electrophoresis of total proteins was performed in order to observe the role of different nitrogen sources on the growth and development of the explants cultivated 'in vitro'. The concentratios of nitrate and ammonium that showed the best results were selected and used in an experiment with different concentrations of NAA and BAP to observe the shoot production. Each experiment was analised at the 30 th , 60 th and 90 th days of culture. The biochemical analysis was performed only at 90 th day of cultive. Concentrations from 26 to 31mmol.L -1 of NO3 - and lower than 5mmol.L -1 of NH4 + were the most effective for growth and development of the explants. However, the experiment with different concentrations of NNA and BAP showed that the best shoot production was achieved on 22,5mmol.L -1 of NO3- + 2,5mmol.L -1 of NH4 + and on 25,0mmol.L -1 of NO3 - + 5,0mmol.L -1 of NH4 + and with 2,0mg.L -1 of BAP + 1,0mg.L -1 of NNA.
Borsoi, Nice Livio. "Desinfecção de explantes e cultivo in vitro de Piretro da Dalmácia (Chrysanthemum cinerariaefolium Vis. cv. Vacaria)." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-05082009-103629/.
Full textThe activities that involve floriculture have distinguished positively in the economic scenario of the Brazilian agribusiness. Among the plant species used for that purpose it can be distinguished the species belonging to the botanical family Asteraceae, mainly from the genus Chrysanthemum. The species C. cinerariaefolium Vis. is worldwide cultivated in order to produce the alelochemicals, which have activities of natural insecticides (pyretrine). In Brazil it was produced in large scale, in the Taquara-RS County in the decade of 30 to 40. Recently the interest on its cultivation has increased as natural alternative of pest management as ornamental plant, mainly in yards. However, the fact of no seed is produced by the plant that limits its capacity of massal production. In order to optimize its micropropagation two experiments were developed. In the experiment 1 it was evaluated 30 days after cultivation the influence of five concentrations of sodium hipochlorine (10, 20, 30, 40 and 50% of the commercial product Mazzarolo), and five time lengths of the explants permanence in the disinfecting solution (5, 10, 15, 20 and 25 min.). It was isolated 125 meristems, 25 treatments with five replications. The isolated explants were inoculated in isolation media, that was constituted of MS + 1.0 mg.mL-1 of BAP + 0.1 mg.L-1 of GA3 + 0.01 mg.L-1 ANA + 30 g.L- 1 of sucrose in 7.0 g.L-1 of Agar. In the experiment 2 it was evaluated the influence of different concentrations of hipochlorine and two length of time in disinfection solution measuring the percentage of necrosis, amorphous structures, seedlings formed and length of the sprouts. The regenerated seedlings from experiment 1 were inoculate in a medium of multiplication that was of MS + 0.4 mg.L-1 of Thiamine HCl + 100 mg.L-1 of Mio-Inositol 0.05 mg.L-1 of ANA + 1.0 mg.L-1 of BAP + 40 g.L-1 of sucrose and 7.0 g.L-1 of Agar. The pH of the medium was adjusted to 5.9 before the autoclaving process. The results obtained in the experiment 1 indicated that the higher the time length in the solution, the higher is the percentage of necrosis. However, the higher is the permanence of higher rate of microbial contamination. In the experiment 2; it was observed that the percentage of necrosis is higher at the concentration of 50%. There was no influence of the analyzed factors in the yield of amorphous structures. For the number of sprouts/explants and length of sprouts, the better performance was in the solution of 30%. However, the rate of sprouts per explants diminished as the length of time in the solution increased. The best results for sprout length were obtained up to 15 minutes of permanence. Therefore, it can be concluded that C. cinerariaefolium Vis. responds positively to in vitro culture. The rate of necrosis increases as the hipochlorine concentration is higher. On the other hand, the contamination rate increases as the disinfection solution diminishes. The concentration o hipochlorine sodium as the length of time of permanence in the initial disinfecting solution used during the explants disinfection affects the number and the sprout length produced during the subculture.
Lelu, Marie-Anne. "Embryogenese somatique chez picea abies l. Karst. A partir de cotyledons de jeunes plantes." Paris 6, 1987. http://www.theses.fr/1987PA066485.
Full textCrespo, Rodríguez Noé Cuauhtémoc. "An examination of behavioral, psychological, socio-cultural and environmental factors that may explain gender differences in children's differences in children's physical activity." Diss., [La Jolla] : [San Diego] : University of California, San Diego ; San Diego State University, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p3386765.
Full textTitle from first page of PDF file (viewed February 3, 2010). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 139-150).
Brockhill, Aneta. "How does the analysis of structural violence help to explain the persistence of the Israel-Palestine conflict? : a case study of the barrier." Thesis, University of Plymouth, 2017. http://hdl.handle.net/10026.1/9289.
Full textAlejos, Marcos. "Transient Expression of BABY BOOM, WUSCHEL, and SHOOT MERISTEMLESS from Virus-Based Vectors in Cotton Explants: Can We Accelerate Somatic Embryogenesis to Improve Transformation Efficiency?" Thesis, University of North Texas, 2019. https://digital.library.unt.edu/ark:/67531/metadc1609070/.
Full textGraff, Curt Gerard. "Course selection theory and college transition seminars: an adaptation of college choice models to explain first-year students' course enrollment behavior." Diss., University of Iowa, 2011. https://ir.uiowa.edu/etd/1141.
Full textCHOUFFOT, STRUYCKEN BARBARA. "Contribution a l'etude des capacites de multiplication vegetative du shorea curtisii dyer ex king, diphterocarpaceae de malaisie." Strasbourg 1, 1986. http://www.theses.fr/1986STR13138.
Full textBrodlie, Malcolm James. "Development of a primary airway epithelial cell culture model and explanted tissue archive to study the role of neutrophilic inflammation and airway remodelling in cystic fibrosis lung disease." Thesis, University of Newcastle Upon Tyne, 2011. http://hdl.handle.net/10443/1228.
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