Academic literature on the topic 'Exosome purification'
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Journal articles on the topic "Exosome purification"
Huang, Kun, Sudha Garimella, Alyssa Clay-Gilmour, Lucia Vojtech, Bridget Armstrong, Madison Bessonny, and Alexis Stamatikos. "Comparison of Human Urinary Exosomes Isolated via Ultracentrifugation Alone versus Ultracentrifugation Followed by SEC Column-Purification." Journal of Personalized Medicine 12, no. 3 (February 24, 2022): 340. http://dx.doi.org/10.3390/jpm12030340.
Full textCrossland, Rachel E., Jean Norden, Louis Bibby, Joanna Davis, and Anne M. Dickinson. "Validation of Isolation Methodology and Endogenous Control Selection for qRT-PCR Assessment of Microrna Expression in Serum and Urine Exosomes." Blood 124, no. 21 (December 6, 2014): 5793. http://dx.doi.org/10.1182/blood.v124.21.5793.5793.
Full textKluszczyńska, Katarzyna, Liliana Czernek, Wojciech Cypryk, Łukasz Pęczek, and Markus Düchler. "Methods for the Determination of the Purity of Exosomes." Current Pharmaceutical Design 25, no. 42 (January 7, 2020): 4464–85. http://dx.doi.org/10.2174/1381612825666191206162712.
Full textPammi Guru, Krishna Thej, Jamuna Surendran Sreeja, Dhrishya Dharmapal, Suparna Sengupta, and Palash Kumar Basu. "Novel Gold Nanoparticle-Based Quick Small-Exosome Isolation Technique from Serum Sample at a Low Centrifugal Force." Nanomaterials 12, no. 10 (May 13, 2022): 1660. http://dx.doi.org/10.3390/nano12101660.
Full textMacías, Mónica, Vera Rebmann, Beatriz Mateos, Nerea Varo, Jose Luis Perez-Gracia, Estibaliz Alegre, and Álvaro González. "Comparison of six commercial serum exosome isolation methods suitable for clinical laboratories. Effect in cytokine analysis." Clinical Chemistry and Laboratory Medicine (CCLM) 57, no. 10 (September 25, 2019): 1539–45. http://dx.doi.org/10.1515/cclm-2018-1297.
Full textVinduska, Vojtech, Caleb Edward Gallops, Ryan O’Connor, Yongmei Wang, and Xiaohua Huang. "Exosomal Surface Protein Detection with Quantum Dots and Immunomagnetic Capture for Cancer Detection." Nanomaterials 11, no. 7 (July 18, 2021): 1853. http://dx.doi.org/10.3390/nano11071853.
Full textLee, Jaewook, Ji-Heon Lee, Jagannath Mondal, Joon Hwang, Han Sang Kim, Vinoth Kumar, Akhil Raj, Seung Rim Hwang, and Yong-Kyu Lee. "Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes." International Journal of Molecular Sciences 23, no. 17 (August 25, 2022): 9619. http://dx.doi.org/10.3390/ijms23179619.
Full textPregnolato, Francesca, Lidia Cova, Alberto Doretti, Donatella Bardelli, Vincenzo Silani, and Patrizia Bossolasco. "Exosome microRNAs in Amyotrophic Lateral Sclerosis: A Pilot Study." Biomolecules 11, no. 8 (August 16, 2021): 1220. http://dx.doi.org/10.3390/biom11081220.
Full textKuleshova, A. E., L. V. Purvinsh, E. E. Burkova, A. E. Grigorieva, E. G. Evtushenko, G. A. Stepanov, E. I. Ryabchikova, and G. A. Nevinskii. "Horse Milk Exosomes: Isolation, Microscopic and Biochemical Analysis, and Prospects of Use." Biotekhnologiya 36, no. 5 (2020): 62–71. http://dx.doi.org/10.21519/0234-2758-2020-36-5-62-71.
Full textRutherford, Sarah C., Seema Sawh, Ashish Saxena, Jennifer Ishii, Maria D. Dominguez, Nyasha Chambwe, Eloisi Caldas Lopes, Xabier Agirre, Doron Betel, and Rita Shaknovich. "Characterization of DLBCL-Derived Exosomes and Investigation of Their Biological Properties." Blood 124, no. 21 (December 6, 2014): 3021. http://dx.doi.org/10.1182/blood.v124.21.3021.3021.
Full textDissertations / Theses on the topic "Exosome purification"
Grunt, Magdalena [Verfasser], and Heidi [Akademischer Betreuer] Schwarzenbach. "Establishment of new assays of exosome purification and microRNA isolation and quantification / Magdalena Grunt ; Betreuer: Heidi Schwarzenbach." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2020. http://d-nb.info/1203301472/34.
Full textGrunt, Magdalena Verfasser], and Heidi [Akademischer Betreuer] [Schwarzenbach. "Establishment of new assays of exosome purification and microRNA isolation and quantification / Magdalena Grunt ; Betreuer: Heidi Schwarzenbach." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2020. http://nbn-resolving.de/urn:nbn:de:gbv:18-102262.
Full textLuz, Juliana Silva da. "Análise estrutural e funcional de cofatores do exossomo em Saccharomyces cerevisiae e Pyrococcus." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-29092006-124545/.
Full textThe synthesis of ribosomes is one of the major metabolic pathways in eukaryotic cells. This process starts in the nucleolus and ends with the export and final maturation of the ribosomal subunits 40S and 60S in the cytoplasm. Three eukaryotic ribosomal RNAs (18S, 5.8S and 25S) are synthesized as a 35S primary transcript (35S pre-rRNA), which is then processed by a complex and ordered series of nucleotide modifications and endo- and exonucleolytic cleavage reactions. These processing reactions depend on 170 proteins, 80 small nucleolar RNAs and specific pre-rRNA sequences. The trans-acting factors, that take part in the processing can be grouped as RNA-helicases, endonucleases, snoRNPs (small nucleolar ribonucleoprotein complexes) and exonucleases, including the exosome. The yeast exosome is composed of 10 essential proteins that function in the processing of rRNAs, snRNAs, snoRNAs and in the degradation of aberrant mRNAs. Recently, the archaeal exosome structure was determined, but no information is yet available on the regulation of the exosome function or on the possible role of the cofactors that transiently interact with it. The main goals of this work were the functional characterization of the protein components of the Saccharomyces cerevisiae exosome RNase PH ring, as well as the structural and functional characterization of the possible cofactors of that complex, Nop17p and Ylr022p. Since the recent characterization of the Pyrococcus exosome, the study of the archaeal exosome cofactors, Pab418p, Pab1135p and aNip7p, was also included in this work, in order to correlate the data on the complex of these different organisms. Our results show that the exonucleolytic activity of the yeast exosome is dependent on the heterodimers formation, as described for archaea. Although it is not clear how Nip7p affects the exosome function in yeast, aNip7p binds RNA and inhibits a-exosome activity in vitro. Yeast Ylr022p binds RNA inespecificaly in vitro, but coprecipitates specific RNAs more efficiently from total cell extracts. Its archaeal orthologue, Pab418p, also binds RNA, but does not affect significantly a-exosome function.
Menino, Glaucia Freitas. "Estudo do exossomo de Archaea e de sua interação com a proteína reguladora PaNip7." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-06042016-143157/.
Full textThe exosome is a multiprotein complex evolutionarily conserved from archaea to higher eukaryotes that performs essential cellular functions such as: 3\'→5\' exoribonucleolytic activity, regulation of mRNA levels, maturation of structural RNAs and quality control of RNAs during the various stages of the gene expression mechanism. In Archaea, the exosome is composed of up to four different subunits, two with RNase PH domains, aRrp41 and aRrp42, and two with RNAs binding domains, aCsl4 and aRrp4. Three copies of the aRrp4 and/or aCsl4 proteins associate with the hexameric catalytic core of the RNase PH ring and complete the formation of the complex. The PaNip7 protein is a regulating cofactor of the Pyrococcus abyssi archaeal exosome and acts in the inhibition of the enzyme complex by binding simultaneously to the exosome and RNAs. In this project, the reconstitution in vitro of the Pyrococcus abyssi archaeal exosome formed by the PaCsl4 top protein was achieved. To this end protein interaction analyses were performed using affinity chromatography, gel filtration and SDS-PAGE techniques. In addition to the formation of the PaCsl4-exosome isoform, a peptide fragment corresponding to the C-terminal region of PaNip7 was synthesized by solid-phase method, purified by RP-HPLC and the purified peptide was characterized by LC/ESI-MS aiming to perform future binding experiments with the exosome.
de, Boer Candice. "Development of a multi-stage purification process for serum-derived exosomes and evaluation of their regenerative capacity." Doctoral thesis, Faculty of Health Sciences, 2020. http://hdl.handle.net/11427/32229.
Full textChao, Pei-Yu, and 巢沛禹. "Exosomes purification based on PEG coated Fe3O4." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/02074383761495791195.
Full text中原大學
機械工程研究所
104
Cancer also known as malignant tumors, is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body, for many years, cancer is a disease of the highest mortality, over 100 cancers affect humans. When cancer metastasis, cancer cells secretes a kind of microvesicles called “Exosome” to create the microenvironment at the destination. So, if we can detect the quantity of exosomes in human body, perhaps have the opportunity to check out the cancer at an early stage. Exosomes appears in biological fluids like blood, but it is impurity that contain lots of protein like globulin and albumin, will reduce the accuracy of liquid biopsy. So this study focuses on exosome isolation with magnetic nanoparticles. The use of chemical co-precipitation method to synthesize Polyethylene glycol(PEG)-coated Fe3O4 nanoparticles, the surface of the Fe3O4 has the reticular structure of PEG, can produce space crowing effect to make protein precipitated from the original location. The particles are uniformly mixed with serum, let particles catch protein, then use magnet to separate the particles and the supernatant, the supernatant contain pure exosomes. In order to use the protein precipitation, the purification of exosomes can be achieved. The diameter of PEG-coated Fe3O4 nanoparticles is about 20 nm, and the agglomerates size is about several hundred nanometers. The use of FESEM, XRD and FTIR to check the PEG is indeed coated on the surface of Fe3O4 nanoparticles. In the experiment of protein isolation, through ELISA to detect, the particles can decline the protein concentration to 39.9% of stock solution. To confirm exosomes are still in the supernatant, the result of Nanosight shows the supernatant content 1.6x1010 particles/ml the size of 30-200nm inclusions, same as the stock solution, SDS-PAGE shows the CD63 exosomes band (~53kD) both are equally show in supernatant and stock solution clearly, the immunoglobulin and albumin band (160~188kD and ~66kD) are shown in stock solution but disappear in supernatant, the result shows the exosome still in the supernatant but the other impurity protein are filtered. As a result, the PEG-coated Fe3O4 nanoparticles indeed can catch the protein and retain the exosome, this is a practical method in exosome isolation.
Gu, Yuan-Huang, and 古原煌. "The Development of an Exosomes Purification and Inspection System." Thesis, 2017. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22105CYCU5489074%22.&searchmode=basic.
Full text中原大學
機械工程研究所
105
For a long time, cancer is a disease that leads to paramount mortality, if we earlier can detect and give appropriate treatment, the more higher survival rate will be greatly improved. When cancer cells begin to hyperplasia or transfer, exosomes will be abnormally secreted in the body. So, if we can develop a simple inspection to detect the quantity of exosomes, perhaps that we just build an early warning for cancers. In this study, we developed a system for purification, dilution sampling of exosomes, and inspection of the number of nanoparticles. First of all, capturing the proteins inside the serum through Polyethylene glycol (PEG)-coated Fe_3 O_4 nanoparticles, then the particles were attached to the bottom by using magnetic force to obtain the exosomes supernatant, the supernatant was diluted with deionized water, and finally put the diluted measuring fluid into the nanoparticles inspection system, the exosomes was measured by rapid image processing after Rayleigh scattered light produced by laser irradiation of exosomes. In this study, the Exosome Purification and Dilution Sampling System are not only able to automatically complete the purification and dilution sampling within 3 minutes, but also can effectively reduce the human factors. Besides, the inspection system only need to improve the traditional microscope, and use laser and 3D printing technology to create a dark field observation platform, to inspect nanoparticles quickly. It has low-cost and easy to popularize characteristics, have the opportunity to develop into an equipment to help humans early detection of cancer cells.
LIU, KANG-YU, and 劉康宇. "Purification and functional study of lung cancer cell exosomes." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/g5s294.
Full text國立聯合大學
化學工程學系碩士班
107
Exosomes are membrane-like-extracellular vesicles with diameter between 20-200nm secreted by most of the eukaryotic cells, which are suggested to have drug resistance effects on cancer cells. Despite a considerable amount of exosomes that could be found in biological medium, the extraction and purification process remains challenging, the high cost and low yield of commercially available kits have limited the applications and development. Here, a novel, affordable and scalable platform was successfully established to effectively purify exosomes from biological medium via precipitation, while preserving the molecular functions of that. In this study, the quality of extraction and purification of exosomes secreted by non-small cell lung cancer PC9 cells were investigated between ExoQuick-TC™, a commercial kits, and polyethylene glycol (PEG) with various molecular weights (Mn), concentrations and pH. Particle size of exosomes purified by all methods were similar, while four-folded yield as well as increased expression of marker protein of exosomes were obtained via 14% PEG with Mn of 8000 at pH 5.4 when compared with ExoQuick-TC™. Besides, PC9 cells pre-treated with exosomes purified by this platform had resulted in 3-5 times increased expression of anti-drug related proteins, 25% reduced apoptosis rate as well as 15% increased cell viability when treated with an anti-cancer drug, gefitinib, indicating that the molecular functions of exosomes purified in this study were preserved.
Book chapters on the topic "Exosome purification"
Lobb, Richard, and Andreas Möller. "Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification." In Methods in Molecular Biology, 105–10. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7253-1_9.
Full textZigáčková, Dagmar, Veronika Rájecká, and Štěpánka Vaňáčová. "Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays." In Methods in Molecular Biology, 237–53. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9822-7_12.
Full textPi, Fengmei, Yangwu Fang, Dongsheng Li, and Peixuan Guo. "Method of Large-Scale Exosome Purification and Its Use for Pharmaceutical Applications." In RNA Nanotechnology and Therapeutics, 355–64. 2nd ed. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003001560-41.
Full textPorrua, Odil. "Purification and In Vitro Analysis of the Exosome Cofactors Nrd1-Nab3 and Trf4-Air2." In Methods in Molecular Biology, 277–89. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9822-7_14.
Full textGonzales, Patricia A., Hua Zhou, Trairak Pisitkun, Nam Sun Wang, Robert A. Star, Mark A. Knepper, and Peter S. T. Yuen. "Isolation and Purification of Exosomes in Urine." In Methods in Molecular Biology, 89–99. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-711-2_6.
Full textMontecalvo, Angela, Adriana T. Larregina, and Adrian E. Morelli. "Methods of Purification of CTL-Derived Exosomes." In Methods in Molecular Biology, 87–102. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1158-5_7.
Full textFridman, Eran, Lana Ginini, Ziv Gil, and Neta Milman. "The Purification and Characterization of Exosomes from Macrophages." In Methods in Molecular Biology, 77–90. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0802-9_6.
Full textDe Gregorio, Cristian, Paula Díaz, Rodrigo López-Leal, Patricio Manque, and Felipe A. Court. "Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs." In Methods in Molecular Biology, 299–315. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7649-2_19.
Full textWierz, Marina, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, and Jerome Paggetti. "Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation." In Methods in Molecular Biology, 231–45. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8885-3_16.
Full textRai, Alin, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson, and David W. Greening. "A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes." In Methods in Molecular Biology, 105–49. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1186-9_9.
Full textConference papers on the topic "Exosome purification"
Inuzuka, Tatsutoshi, Ayako Kurimoto, and Yuki Kawasaki. "Abstract A045: High-purity purification of exosome from multiple specimens and automation of the process." In Abstracts: AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; October 26-30, 2019; Boston, MA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1535-7163.targ-19-a045.
Full textMandrekar, Michelle, Jami English, Douglas Horejsh, Chris Moreland, Herly Karlen, and Marjeta Urh. "Abstract 4426: Automated miRNA purification from plasma, serum or exosomes." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-4426.
Full textCappione, Amedeo, Sara Gutierrez, Masaharu Mabuchi, Janet Smith, Ivona Strug, and Timothy Nadler. "Abstract 3483: A centrifugal ultrafiltration-based method for rapid purification of exosomes from biological samples." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3483.
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