Academic literature on the topic 'Ethylenediaminetetraacetic acid (EDTA)'
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Journal articles on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Mohammadi, Zahed, Sousan Shalavi, and Hamid Jafarzadeh. "Ethylenediaminetetraacetic acid in endodontics." European Journal of Dentistry 07, S 01 (September 2013): S135—S142. http://dx.doi.org/10.4103/1305-7456.119091.
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ABSTRACTEthylenediaminetetraacetic acid (EDTA) is a chelating agent can bind to metals via four carboxylate and two amine groups. It is a polyamino carboxylic acid and a colorless, water-soluble solid, which is widely used to dissolve lime scale. It is produced as several salts, notably disodium EDTA and calcium disodium EDTA. EDTA reacts with the calcium ions in dentine and forms soluble calcium chelates. A review of the literature and a discussion of the different indications and considerations for its usage are presented.
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Coyne, Erwin, Harry L. Messmore, Jeanine M. Walenga, Jawed Fareed, and William H. Wehrmacher. "Ethylenediaminetetraacetic Acid (EDTA) in Heparin." Thrombosis Research 90, no. 5 (June 1998): 245–46. http://dx.doi.org/10.1016/s0049-3848(98)00063-2.
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Chen, Min, and R. Stephen Reid. "Solution speciation in the aqueous Na(I)–EDTA and K(I)–EDTA systems." Canadian Journal of Chemistry 71, no. 5 (May 1, 1993): 763–68. http://dx.doi.org/10.1139/v93-100.
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Speciation in the aqueous sodium(I) – ethylenediaminetetraacetic acid and potassium(I) – ethylenediaminetetraacetic acid equilibrium systems was investigated by automated gravimetric potentiometric titration. It was found that in both cases, particularly below neutral pH values, speciation includes a previously uncharacterized diprotonated complex. All formation and acid dissociation constants were measured for the full systems. The validity of previous literature equilibrium constants, which are based on incomplete equilibrium models, is discussed.
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Nakatsu, K., M. Vandenberghe, S. Kobus, J. Kawamoto, J. F. Brien, and G. S. Marks. "Endothelium-dependent relaxation of rabbit aorta by acetylcholine requires ethylenediaminetetraacetic acid." Canadian Journal of Physiology and Pharmacology 64, no. 7 (July 1, 1986): 1050–52. http://dx.doi.org/10.1139/y86-179.
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Experiments were conducted to determine if ethylenediaminetetraacetic acid (EDTA) was essential for the acetylcholine (ACh)-induced relaxation of blood vessels. Isolated rabbit aortic rings were prepared for recording isometric tension. They were maintained in Krebs bicarbonate solution with various concentrations of EDTA. With EDTA concentrations of 0 or 0.003 mM, no ACh-induced relaxation was observed; only the contractile effect of ACh was seen. With 0.03 and 0.30 mM EDTA, Ach induced relaxation with EC50 values of 0.11 and 0.098 μM, respectively. Under the experimental conditions used, EDTA was essential for demonstration of ACh-induced relaxation.
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Andreev, Irina, Valeria Gabechaya, and Oksana Smelykh. "Evaluation of the spring rapeseed use efficiency in the technology of induced nickel phytoextraction under conditions of polluted Albic Luvisol." АгроЭкоИнфо 4, no. 52 (August 18, 2022): 17. http://dx.doi.org/10.51419/202124417.
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A greenhouse experiment was conducted to study the effect of chelating agent disodium salt of ethylenediaminetetraacetic acid (EDTA) on nickel phytoextraction with rapeseed plants (Brassica napus (L.) subsp. oleifera Metzg.) as test crop in the contaminated with Ni (40 and 120 mg/kg) Albic Luvisol. The use of EDTA at a dose of 0.5 mmol/kg of soil increased the nickel uptake by plants by 1.9 - 2.4 times compared to EDTA unamended soil. Due to the absence of phytotoxic effect, values of uptake, bioaccumulation and translocation coefficients, the highest efficiency of nickel phytoextraction by rapeseed plants was achieved at doses of metal in the soil at the level of approximately permissible concentration (40 mg/kg). Keywords: PHYTOREMEDIATION, INDUCED PHYTOEXTRACTION, NICKEL, CHELATING AGENT, DISODIUM SALT OF ETHYLENEDIAMINETETRAACETIC ACID (EDTA), SPRING RAPESEED, POLLUTION, ALBIC LUVISOL
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Kopermsub, P., V. Mayen, and C. Warin. "Nanoencapsulation of Nisin and Ethylenediaminetetraacetic Acid in Niosomes and Their Antibacterial Activity." Journal of Scientific Research 4, no. 2 (April 27, 2012): 457. http://dx.doi.org/10.3329/jsr.v4i2.9409.
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Ethylenediaminetetraacetic acid or EDTA was used complementarily with nisin to give a synergistic antibacterial effect against Gram positive and negative bacteria. Nisin and EDTA were encapsulated in nonionic surfactant vesicles or niosomes. Sorbitan monooleate and polyethylene glycol were precursors in preparation of niosomes. Size reduction of niosomes was conducted via extrusion through polycarbonate membrane with pore size of 200 nm. Diameters of prepared blank niosomes and nisin-EDTA-encapsulated niosomes were approximately 130 nm and 270 nm, respectively. Bilayer structure of niosomes was observed from negative staining-transmission electron microscopic images. Long-termed investigation of antimicrobial activity of nisin-EDTA-encapsulated niosomes and free nisin/EDTA were conducted against Staphylococcus aureus and Escherichia coli. Bacterial counts denoted a slow release of nisin-EDTA-encapsulated niosomes overtime whilst free nisin/EDTA gave a sudden bactericidal activity. Due to that free nisin/EDTA was immediately exploited at the beginning, bacterial counts then tended towards higher during the latter time of antimicrobial activity test. Keywords: antibacterial; EDTA; Escherichia coli; niosomes; nisin; Staphylococcus aureus. © 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v4i2.9407 J. Sci. Res. 4 (2), 457-465 (2012)
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Su, En-Chin, Ju-Ting Lee, Yi-Jean Gong, Bing-Shun Huang, and Ming-Yen Wey. "Photocatalytic conversion of ethylenediaminetetraacetic acid dissolved in real electroplating wastewater to hydrogen in a solar light-responsive system." Water Science and Technology 77, no. 12 (June 18, 2018): 2851–57. http://dx.doi.org/10.2166/wst.2018.276.
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Abstract A sustainable and multifunctional photocatalysis-based technology has been established herein for simultaneous hydrogen generation and oxidation of ethylenediaminetetraacetic acid (EDTA) in real electroplating wastewater. When the photocatalyst concentration was 4 g/L and electroplating wastewater pH was 6, optimal adsorptions of EDTA2−, H+, and H2O were observed, while hydrogen generation efficiency reached 305 µmol/(h g). Owing to EDTA oxidation and occupation of the active sites of the photocatalyst by Ni ions or Ni-EDTA chelates, the charge separation and adsorptions of H+ and H2O decreased, reducing hydrogen generation efficiency with time. The lower EDTA and Ni concentrations in treated wastewater showed that photocatalytic conversion of EDTA in real electroplating wastewater to enhance hydrogen generation efficiency can be a practical alternative energy production technology. This study provided a novel idea to enhance the value of electroplating wastewater, to build a hydrogen generation route with no consumption of a valuable resource, and to reduce EDTA and Ni concentrations in electroplating wastewater.
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Moonprasith, N., A. Poonsrisawat, V. Champreda, C. Kongkaew, S. Loykulnant, and K. Suchiva. "Deproteinization of Nonammonia and Ammonia Natural Rubber Latices by Ethylenediaminetetraacetic Acid." Advances in Materials Science and Engineering 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/1516945.
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Deproteinized natural rubber (DPNR) was made from sodium hydroxymethylglycinate latex (SH-latex) and ammonia latex (NH3-latex) by mixing with different forms of ethylenediaminetetraacetic acid (EDTA) in the presence of sodium dodecyl sulfate (SDS). The mixtures were stirred at room temperature followed by centrifugation to separate the denatured proteins. The optimized reaction contained 0.01 wt% ethylenediaminetetraacetic acid tetrasodium (EDTA-4Na) with 1% SDS. The nitrogen contents of the DPNR from SH-latex and NH3-latex were reduced to 0.005 wt% and 0.008 wt%, respectively, compared to 0.551 wt% in the starting SH-latex and 0.587 wt% in the NH3-latex. SDS-PAGE analysis and FT-IR spectroscopy showed decomposition of latex proteins to peptides of smaller molecular weight. Physical properties of the DPNR rubber were studied. The novel EDTA-4Na treatment is considered an effective deproteinization method with potential application on both ammonia and nonammonia preservative systems.
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Ramadan, Abd El-Motaleb M., Shaban Y. Shaban, Mohamed M. Ibrahim, Adel A. H. Abdel-Rahman, Shehab A. Sallam, Sami A. Al-Harbi, and Walid Omar. "Synthesis and spectroscopic characterization of ternary copper(ii) complexes containing nitrogen and oxygen donors as functional mimics of catechol oxidase and phenoxazinone synthase." New Journal of Chemistry 44, no. 16 (2020): 6331–45. http://dx.doi.org/10.1039/c9nj06131b.
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Two ternary copper(ii) complexes 1 [(Cu2Me4en)4(EDTA)] and 2 [(CuMe4en)2(MIDA)] containing the mixed ligand system of 1,1′,4,4′-tetramethylethylenediamine (Me4en) (L) and N-methyliminodiacetic (MIDAH2) (L′) or ethylenediaminetetraacetic acid (EDTAH4) (L′) were synthesized.
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Marcia, Mario, Prabhpreet Singh, Frank Hauke, Michele Maggini, and Andreas Hirsch. "Novel EDTA-ligands containing an integral perylene bisimide (PBI) core as an optical reporter unit." Org. Biomol. Chem. 12, no. 36 (2014): 7045–58. http://dx.doi.org/10.1039/c4ob01007h.
Full textDissertations / Theses on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Ahmad, Salman. "Raman spectroscopic evidence for side-on binding of peroxide ion to ferric EDTA." Full text open access at:, 1988. http://content.ohsu.edu/u?/etd,177.
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Fauconnier, Theresa K. "Azapropazone and derivatized EDTA and DTPA complexes as MRI contrast agents /." *McMaster only, 1996.
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Fang, Bin. "Remobilisation of Heavy Metals from Sediments Using Aminopolycarboxylic Acids." Thesis, The University of Sydney, 2005. http://hdl.handle.net/2123/630.
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This thesis describes a study of the remobilisation of heavy metals from sediments by three aminopolycarboxylic acids (APCAs). They are nitrilotriacetic acid, ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid. The investigation is introduced by examining the sources, uses and chemistry of these acids. The introduction also includes a discussion of what is known about the inclusion of heavy metals into sediments and their remobilisation from sediments. Typical concentrations of APCAs in natural waters and sediments have been catalogued from the literature. The advantages and disadvantages of various laboratory techniques employed for the remobilisation of heavy metals by APCAs from sediments are assessed, as is the use of such experiments in quantifying the role of APCAs in the remobilisation of heavy metals from sediments. Sediments from three areas were sampled for this study; they were the Alexandra Canal, Captains Flat and Jenolan Caves in New South Wales, Australia. In each area several sites were sampled. For each site there is a brief description of the catchment geology and hydrology. Selected sediment-associated waters in the areas were analysed for their metal concentrations as well as for ultratrace levels of APCAs employing a method developed in the present study. The waters were analysed for the major ions Ca2+, Mg2+, K+, Na+, Cl-, NO3- and SO42-. The sediments from selected sites in each of the areas were dried and fractionated. The dry total and fine sediments were analysed for their metal content and the latter was found to adequately represent the former in this respect. Water samples from the three areas showed different chemistries and exhibited more subtle differences between sites. In general, the Alexandra Canal waters are saline and alkaline and are a mixture of urban runoff and seawater; the Captains Flat waters are acidic and contain high sulfate from acid mine and tailings drainage; the Jenolan Caves waters are neutral and have the features characteristic of waters draining through limestone. The APCA contamination in all water samples when ranked against other global sites is very low. Although the current APCA levels in the waters appear low, it was concluded that they should be closely monitored so that efforts can be made to minimise the risk of APCAs being hazardous environmental contaminants and also that any remobilisation of heavy metals from sediments by APCAs can be controlled. Agitation and column laboratory-scale experiments were carried out in order to obtain an understanding of the remobilisation of metals by contamination levels of APCAs in water, both as the individual APCAs and as a mixture of APCAs. Complimentary experiments were carried out using a molar excess of APCAs calculated from the metal concentrations obtained by acid digestion (assuming 1:1 metal complex formation). Both types of remobilisation experiments were designed to investigate the role that redox potential (Eh) and concentration of APCAs in natural waters have on the remobilisation of heavy metals from the sediments. The agitation experiments were employed to assess metal remobilisation for the situation where the sediments are disturbed while the column experiments explored metal remobilisation for the case where the sediments are left undisturbed in situ. The major conclusions from the agitation experiments that used fine sediment from the Alexandra Canal were that 100 ppm APCA solutions will remobilise metals from the sediments under oxic conditions but only remobilise infinitesimally small amounts of metal under anoxic conditions. The use of fine sediments for the duplicate agitation experiments was found to give adequate duplication of results. A mixture of APCAs in solution acts similarly to the average of the three individual APCA solutions, showing that there are no antagonistic or synergistic effects likely to occur when they are found together in the environment. It was found that the mmoles of the metals remobilised exceeded the mmoles of the APCAs added when 500.0 mL of 100 ppm APCA solution was used on 50.00 g of sediment. This might be due to APCAs remobilising metals from the sediments in ways other than by complexation. Even though an excess of APCAs was available, metal remobilisation was not complete when the experiments were forced to terminate. During the 14 days of the experiment, only one quarter of the metals liberated from the sediment by HNO3 and 30 % H2O2 digestion were remobilised by the APCAs. Therefore an excess of free APCAs remains in solution. Fine sediments from Alexandra Canal, Captains Flat and Jenolan Caves were employed in the oxic agitation experiments using excess APCAs in solution. These experiments resulted in the following major conclusion: when producing an APCA remobilisation signature for trace and ultratrace metals, the geochemistry of the site is of secondary importance to the source of the contaminating metals. This is a feature of the trace and ultratrace metal speciation in the source rather than their concentration in it. From the different levels of calcium present in the three areas it was found that calcium is unlikely to form stable 1:1 APCA complexes at the pH values employed and is unlikely to compete with the heavy metal remobilisation by APCAs. Total sediments from Alexandra Canal and 100 ppm APCA solutions were employed for the column leaching experiments. From mass, pore water volumes and flow measurements it was shown that the ten mini cores taken from the same site were not true replicates. Despite this, when the sediments have settled and the pore waters removed from the cores, the levels of metal being leached stabilise and may represent a clearer picture of the in situ metal leaching from sediment with time. The levels of metal leached from the cores in 14 days suggest that during this period the cores are essentially anoxic, with the oxygen supplied from the oxic leaching solutions used for inorganic and microbial processes in the sediments. Agitation experiments appeared to yield an adequate picture of what would happen if free APCA solution came in contact with fine sediments suspended in the water column. Column leaching experiments employing total sediment were found to be only of limited value in assessing heavy metal remobilisation from undisturbed sediment. These experiments do not give a reliable assessment of the bioavailability of heavy metals and further testing of the acute and chronic toxicity of the sediments is recommended. APCA solutions that have been used in sediment and soil washing under conditions related to those used in the present study may contain an excess of the free APCAs as well as APCA heavy metal complexes and hence may be toxic to biota.
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Fang, Bin. "Remobilisation of Heavy Metals from Sediments Using Aminopolycarboxylic Acids." University of Sydney. Chemistry, 2005. http://hdl.handle.net/2123/630.
Full textAbstract:
This thesis describes a study of the remobilisation of heavy metals from sediments by three aminopolycarboxylic acids (APCAs). They are nitrilotriacetic acid, ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid. The investigation is introduced by examining the sources, uses and chemistry of these acids. The introduction also includes a discussion of what is known about the inclusion of heavy metals into sediments and their remobilisation from sediments. Typical concentrations of APCAs in natural waters and sediments have been catalogued from the literature. The advantages and disadvantages of various laboratory techniques employed for the remobilisation of heavy metals by APCAs from sediments are assessed, as is the use of such experiments in quantifying the role of APCAs in the remobilisation of heavy metals from sediments. Sediments from three areas were sampled for this study; they were the Alexandra Canal, Captains Flat and Jenolan Caves in New South Wales, Australia. In each area several sites were sampled. For each site there is a brief description of the catchment geology and hydrology. Selected sediment-associated waters in the areas were analysed for their metal concentrations as well as for ultratrace levels of APCAs employing a method developed in the present study. The waters were analysed for the major ions Ca2+, Mg2+, K+, Na+, Cl-, NO3- and SO42-. The sediments from selected sites in each of the areas were dried and fractionated. The dry total and fine sediments were analysed for their metal content and the latter was found to adequately represent the former in this respect. Water samples from the three areas showed different chemistries and exhibited more subtle differences between sites. In general, the Alexandra Canal waters are saline and alkaline and are a mixture of urban runoff and seawater; the Captains Flat waters are acidic and contain high sulfate from acid mine and tailings drainage; the Jenolan Caves waters are neutral and have the features characteristic of waters draining through limestone. The APCA contamination in all water samples when ranked against other global sites is very low. Although the current APCA levels in the waters appear low, it was concluded that they should be closely monitored so that efforts can be made to minimise the risk of APCAs being hazardous environmental contaminants and also that any remobilisation of heavy metals from sediments by APCAs can be controlled. Agitation and column laboratory-scale experiments were carried out in order to obtain an understanding of the remobilisation of metals by contamination levels of APCAs in water, both as the individual APCAs and as a mixture of APCAs. Complimentary experiments were carried out using a molar excess of APCAs calculated from the metal concentrations obtained by acid digestion (assuming 1:1 metal complex formation). Both types of remobilisation experiments were designed to investigate the role that redox potential (Eh) and concentration of APCAs in natural waters have on the remobilisation of heavy metals from the sediments. The agitation experiments were employed to assess metal remobilisation for the situation where the sediments are disturbed while the column experiments explored metal remobilisation for the case where the sediments are left undisturbed in situ. The major conclusions from the agitation experiments that used fine sediment from the Alexandra Canal were that 100 ppm APCA solutions will remobilise metals from the sediments under oxic conditions but only remobilise infinitesimally small amounts of metal under anoxic conditions. The use of fine sediments for the duplicate agitation experiments was found to give adequate duplication of results. A mixture of APCAs in solution acts similarly to the average of the three individual APCA solutions, showing that there are no antagonistic or synergistic effects likely to occur when they are found together in the environment. It was found that the mmoles of the metals remobilised exceeded the mmoles of the APCAs added when 500.0 mL of 100 ppm APCA solution was used on 50.00 g of sediment. This might be due to APCAs remobilising metals from the sediments in ways other than by complexation. Even though an excess of APCAs was available, metal remobilisation was not complete when the experiments were forced to terminate. During the 14 days of the experiment, only one quarter of the metals liberated from the sediment by HNO3 and 30 % H2O2 digestion were remobilised by the APCAs. Therefore an excess of free APCAs remains in solution. Fine sediments from Alexandra Canal, Captains Flat and Jenolan Caves were employed in the oxic agitation experiments using excess APCAs in solution. These experiments resulted in the following major conclusion: when producing an APCA remobilisation signature for trace and ultratrace metals, the geochemistry of the site is of secondary importance to the source of the contaminating metals. This is a feature of the trace and ultratrace metal speciation in the source rather than their concentration in it. From the different levels of calcium present in the three areas it was found that calcium is unlikely to form stable 1:1 APCA complexes at the pH values employed and is unlikely to compete with the heavy metal remobilisation by APCAs. Total sediments from Alexandra Canal and 100 ppm APCA solutions were employed for the column leaching experiments. From mass, pore water volumes and flow measurements it was shown that the ten mini cores taken from the same site were not true replicates. Despite this, when the sediments have settled and the pore waters removed from the cores, the levels of metal being leached stabilise and may represent a clearer picture of the in situ metal leaching from sediment with time. The levels of metal leached from the cores in 14 days suggest that during this period the cores are essentially anoxic, with the oxygen supplied from the oxic leaching solutions used for inorganic and microbial processes in the sediments. Agitation experiments appeared to yield an adequate picture of what would happen if free APCA solution came in contact with fine sediments suspended in the water column. Column leaching experiments employing total sediment were found to be only of limited value in assessing heavy metal remobilisation from undisturbed sediment. These experiments do not give a reliable assessment of the bioavailability of heavy metals and further testing of the acute and chronic toxicity of the sediments is recommended. APCA solutions that have been used in sediment and soil washing under conditions related to those used in the present study may contain an excess of the free APCAs as well as APCA heavy metal complexes and hence may be toxic to biota.
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Wang, Zhongning. "Apparent and partial molar heat capacities and volumes of aqueous chelating agents, EDTA and NTA." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ36191.pdf.
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Herman, Jacob Paul. "Ethylenediaminetetraacetate and nitrilotriacetate degradation by bacterium BNC1 biochemical characterization of the substrate uptake system and cloning of the entire EDTA-degrading gene cluster in Escherichia coli /." Online access for everyone, 2004. http://www.dissertations.wsu.edu/Dissertations/Summer2004/j%5Fherman%5F051904.pdf.
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Chang, Hyun-Shik. "Determination of oxidation mechanism of ethylenediaminetetraacetate (EDTA)-metal complexes by alkaline permanganate and structures of in situ formed manganese oxides containing heavy metals /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/10106.
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Ferreira, Letícia Bergo Coelho. "Bioquímica do plasma seminal e efeito do edta adicionado ao meio de congelamento do sêmen de cão doméstico." Universidade Federal de Viçosa, 2013. http://locus.ufv.br/handle/123456789/5180.
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One of the main deleterious factors inherent to the process of canine semen freezing is the large amount of sperm membranes lesions observed in the thawing. The freezing induce some destabilization of cell membrane and changes in the conformation between membrane receptors and ionic channels, promoting the calcium influx and increasing the probability of a premature acrosome reaction (AR), that invalidate the fertilizantion of the oocyte. The calcium is the principal inducing agent of the AR in vitro, and there is not recent reports about the biochemical composition of the seminal plasm and about the calcium levels that prevent the premature AR in the frozen of the domestic dog semen too. This study aimed to evaluate the alkaline phosphatase and cationic composition in seminal plasma of the fractions of dog ejaculate; and also test the effects of calcium amount reducing in freezing medium using the EDTA as a chelating agent. The semen was collected from three half-breed dogs. The sperm-rich fraction (SRF) and the prostatic fraction (PF) were separately collected. Both samples were evaluated by spectrophotometry, measuring the concentrations of calcium, sodium, potassium, alkaline phosphatase (AP), and the freezing medium. The fresh semen was evaluated by classical tests (strength, progressive motility, morphology, and membrane integrity). The remainder was divided into three aliquots that were diluted in a freezing medium based on tris-citrate-egg yolk, glycerol and equex with different concentrations of EDTA. The first treatment did not contain EDTA, treatment 2 contained 0.1% EDTA and the third, 0.25%. The most abundant cation in seminal plasma of these dogs was sodium, followed by potassium and calcium, respectively, both in SRF and in PF, without significant difference between the concentrations of these ions in both fractions. The AP obtained in SRF was significantly higher (p <0.000001) than that obtained in PF. There was a high concentration of calcium in the freezing medium based, that was significantly reduced in the treatments with EDTA. Deleterious effects were observed in all analyzed parameters in thawed semen compared with fresh semen (progressive motility, morphology, and membrane integrity). With the use of fluorescent probes, we did not note significant difference in the percentage of the plasmatic membranes e acrosomal membranes injured among the treatments with different EDTA concentrations. The extracellular medium has high calcium concentration, and despite the EDTA had significantly reduced the extracellular calcium in those treatments that it was added, there was not acrosomal membrane injury reduction in these treatments. This may have been caused because the concentration of this cation still remained high in comparision with its concentration in the original seminal plasma. We note too that the EDTA did not presented deleterious effects on the spermatozoa inside the evaluated parameters.
Um dos principais fatores deletérios, inerentes ao processo de congelamento de sêmen canino, é a grande quantidade de lesões de membranas espermáticas observada ao descongelamento. O congelamento induz a desestabilização das membranas celulares e alterações na conformação de receptores e canais iônicos, promovendo influxo de cálcio para o citosol, aumentando a chance de ocorrer prematuramente a reação acrossomal (RA), o que pode inviabilizar a fertilização do ovócito. O cálcio é reconhecido como o principal agente indutor da RA em espermatozoides de mamíferos. Não existem relatos recentes sobre meios de congelamento de sêmen de cão que contenham concentrações de cálcio preventivas à RA prematura. Os objetivos deste estudo foram avaliar quantitativamente a concentração dos cátions cálcio, sódio e potássio e da enzima fosfatase alcalina nas frações dos ejaculados de cães e também testar o efeito da redução da quantidade de cálcio no meio de congelamento utilizando o EDTA como quelante. Utilizou-se ejaculados de três cães mestiços, coletando em separado a fração rica em espermatozoides (FR) e a fração prostática (FP). Ambas foram avaliadas por espectrofotometria para aferição das concentrações dos cátions e da FA, assim como os meios de congelamento utilizados. O sêmen fresco foi avaliado por meio de testes clássicos (vigor, motilidade, hiposmótico e morfologia espermática) e o restante foi dividido em três alíquotas para diluição em meio base de congelamento tris-citrato- gema de ovo com glicerol e equex, meio básico contendo 0,1% de EDTA e meio básico contendo 0,25% de EDTA. O cátion estudado que se apresentou mais abundante no plasma seminal foi o sódio, seguido pelo potássio e o cálcio, tanto na FR quanto na FP, não havendo diferença entre as concentrações em ambas as frações (p >0,05). A FA obtida na FR foi maior que a obtida na FP (p<0,05). Observou-se elevada concentração de cálcio no meio base de congelamento, a qual foi reduzida nos tratamentos com EDTA (p<0,05). Houve efeitos deletérios em todos os parâmetros analisados no sêmen descongelado em comparação com o sêmen fresco (vigor, motilidade, integridade de membrana plasmática e morfologia). Não foi observada diferença na avaliação de lesões nas membranas plasmática e acrossomal com uso de sondas fluorescentes entre os meios com diferentes concentrações de EDTA (p>0,05). Embora o uso de EDTA tenha reduzido o cálcio extracelular, a concentração deste cátion permaneceu alta em comparação com sua concentração original no plasma seminal, fator que pode ser responsável pela não redução de lesões de membrana acrossomal nos tratamentos com EDTA. Por outro lado o EDTA não apresentou efeito deletério sobre os espermatozoides de acordo com os parâmetros avaliados.
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Sillence, Kelly. "Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT) : a comparison of molecular techniques." Thesis, University of Plymouth, 2016. http://hdl.handle.net/10026.1/5319.
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Prenatal assessment of fetal health is routinely offered throughout pregnancy to ensure that the most effective management can be provided to maintain fetal and maternal well-being. Currently, invasive testing is used for definitive diagnosis of fetal aneuploidy, which is associated with a 1% risk of iatrogenic fetal loss. Developing non-invasive prenatal testing (NIPT) is a key area of research and methods to increase the level of cell-free fetal DNA (cffDNA) within the maternal circulation have been discussed to improve accuracy of such tests. In this study, three strategies; co-amplification at lower denaturation temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against real-time PCR (qPCR) was compared for fetal sex and RHD genotyping. In addition RHD zygosity testing was carried out for non-maternal samples. Consequently, Pippin Prep™ gel electrophoresis was combined with ddPCR analysis for the NIPD of Down Syndrome (DS) in pseudo-maternal samples. The results revealed that the Pippin Prep™ gel electrophoresis enrichment approach successfully demonstrated 2-fold to 5-fold increases in the cffDNA fraction. However, further optimisation assays of COLD-PCR and inverse-PCR using actual maternal samples were required. The spike experiments for DS detection revealed that with the present assay IV overrepresentation of the chromosome 21 target could be significantly detected for samples with ≥15% ‘cffDNA fraction’. In conjunction with the Pippin Prep™ enrichment method, this would have enabled assessment of all 10 maternal samples. Alternatively, fetal sex and RHD genotyping results determined that ddPCR provides a more sensitive platform compared to qPCR approaches, particularly for samples that express low cffDNA fractions (<2%). The ddPCR platform also proved to be a rapid and accurate system for the determination of RHD zygosity. This study highlights that ddPCR could be used as opposed to qPCR for accurate determination of fetal sex and RHD status. While sequencing approaches currently provide the most sensitive platforms for NIPT of fetal aneuploidy, high costs (>£400) prevent universal application. The combination of cffDNA enrichment with ddPCR analysis could provide a cheaper and more widely available platform for NIPD. However, further large scale validation studies using actual maternal samples are required.
Books on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Campbell, Colm J. Synthesis and study of platinum(II) and platinum(IV) complexes of EDTA derivatives as potential antitumour agents. Dublin: University College Dublin, 1996.
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Pahlman, J. E. Safe cleaning of State of Maine filters using EDTA-type chelating agents. Pittsburgh, Pa: U.S. Dept. of the Interior, Bureau of Mines, 1986.
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Molnar, Andris. Edta: Synthesis, Uses and Environmental Concerns. Nova Science Publishers, Incorporated, 2013.
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Cranton, Elmer M. A Textbook on Edta Chelation Therapy. 2nd ed. Hampton Roads Publishing Company, 2001.
Find full textBook chapters on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Wolf, K., and P. A. Gilbert. "EDTA—Ethylenediaminetetraacetic Acid." In Detergents, 243–59. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-540-47108-0_7.
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Urist, Marshall R. "Enzymes in Bone Morphogenesis: Endogenous Enzymic Degradation of the Morphogenetic Property in Bone in Solutions Buffered by Ethylenediaminetetraacetic Acid (EDTA)." In Ciba Foundation Symposium 11 - Hard Tissue Growth, Repair and Remineralization, 143–60. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470719947.ch7.
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Barton, C. Charles, and Harihara M. Mehendale. "EDTA (Ethylenediaminetetraacetic Acid)." In Encyclopedia of Toxicology, 147–48. Elsevier, 2005. http://dx.doi.org/10.1016/b0-12-369400-0/00363-x.
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Barton, C. "EDTA (Ethylenediaminetetraacetic Acid)." In Encyclopedia of Toxicology, 310–11. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-386454-3.00309-2.
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"Ethylenediaminetetraacetic Acid (EDTA)." In Food Additives Data Book, 116–17. Oxford, UK: Blackwell Science Ltd, 2007. http://dx.doi.org/10.1002/9780470995327.ch30.
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"Slaying." In Vampirology: The Science of Horror's Most Famous Fiend, 231–50. The Royal Society of Chemistry, 2021. http://dx.doi.org/10.1039/bk9781839161575-00231.
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When all else fails, there is nothing left to do but to destroy troublesome vampires. But how do you kill something that is already dead? A number of methods have been tried and tested over the centuries. Chemical methods of destruction include ethylenediaminetetraacetic acid (EDTA), water and silver bullets. Burning is another popular choice, but just how flammable could a vampire be? The most well known method is, of course, a stake through the heart; but in practice it is far from easy. This chapter explores why are certain types of wood might be favoured in different parts of the world. Sometimes it might be because of their hardness or resistance to splintering, but in other cases there might be extra toxic components that make sure the dead remain so.
Conference papers on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Guerrero, Rodel D., Imee Kassandra E. Cacho, Dan Michael A. Asequia, and Joshua Emmanuel L. Hugo. "Desorption of Lead Ions from Used Sodium Alginate-Hydroxypropyl Cellulose Adsorbent Beads." In 7th GoGreen Summit 2021. Technoarete, 2021. http://dx.doi.org/10.36647/978-93-92106-02-6.7.
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Efficient removal of adsorbed lead ions from the sodium alginate-hydroxypropyl cellulose beads was necessary to guarantee their long-term use for repeated adsorption-desorption cycles. In this study, the desorption characteristics of previously adsorbed lead ions on sodium alginate-hydroxypropyl (SA-HPC) cellulose adsorbent beads were tested using various eluents such as sulfuric acid and ethylenediaminetetraacetic acid (EDTA). SA-HPC adsorption beads were produced using 3:1 ratio of sodium alginate to hydroxypropyl cellulose via ionotropic gelation. Generally, using H2SO4 as eluent maintained an exceptional adsorption efficiency throughout in each cycle, but showed a weak desorption efficiency performance. The desorption efficiency using 0.1M EDTA, on the other hand, was found to be the most effective but resulted to the beads disintegration after the first cycle.
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Qin, Hantang, Jingyan Dong, and Yuan-Shin Lee. "Electrohydrodynamic Jet Printing of Silver Seeds: Micro Scale Patterning by Electroless Copper Deposition." In ASME 2015 International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/msec2015-9487.
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The paper describes a new method for micro scale patterning of highly conductive features on flexible and flat surfaces. The method uses electrohydrodynamic jet printing to deposit silver seeds on-demand that serve as catalysts for subsequent electroless deposition of copper. The electroless deposition of copper on substrates occurred only where silver seeds exist. In the study, ethylenediaminetetraacetic acid (EDTA) and triethanolamine (TEA) were used as chelating agents, and formaldehyde as reducing agent. Copper growth rate and resistivity were investigated using microscopic and profilometer to determine optimal concentration of each agents in reaction solution. The results indicated that EDTA significantly affects copper growth rate, playing an important role in complexing, while TEA in the dual-complexing system will balance deposition rate and stability of solution. Optimal temperature and time for copper deposition on silver nanoparticles were also discussed in the study. The techniques of activating substrates by selective printing and electroless metallization was successfully used to pattern on glass, and flexible polymer films, and both flat and curved substrates were used. The proposed technique was also capable of fabricating metal structures on flexible substrates with excellent conductivity. Metal filaments with resistivity four times bulk copper and thickness up to 15μm were demonstrated in the research.
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Gasimli, Nijat, Mohamed Mahmoud, Amjed Hassan, Muhammed Shahzad Kamal, Shirish Patil, and Ridha Al-Abdrabalnabi. "Iron Sulfide Scale Inhibition Through Adsorption of Chelating Agents." In SPE Annual Technical Conference and Exhibition. SPE, 2022. http://dx.doi.org/10.2118/210275-ms.
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Abstract An important oilfield issue is the formation of a wide range of scales during oil and gas well operations. Oilfield scales hinder assessing an optimum hydrocarbon production as their precipitation on formation, various surface, and downhole equipment leads to many problems, including pressure decrement, formation damage, and operational failure of subsurface equipment. One type of these scales is the iron sulfide scale and based on studies in the Khuff reservoir, iron sulfide scales are likely to deposit on production tubing and rock formation. Therefore, it becomes essential to restrain the occurrence of iron sulfide scale using environmentally friendly chemicals in production tubing, water injection wells, and near-wellbore formation. The primary focus of this work is the prevention of iron sulfide scale deposition in carbonate formations during water injection applications. Iron sulfide scale inhibition was studied through dynamic inhibition adsorption experiments. In contrast to conventional experiments, for scale inhibition and adsorption of chelating agents (static bottle, dynamic filter tube tests) and simulation studies, a novel experimental setup (coreflooding experiments) was proposed to study the inhibitor adsorption. Broad concentrations of high-pH aminocarboxylic acids (such as ethylenediaminetetraacetic acid (ETDA) and diethylenetriamine pentaacetate acid (DTPA)) were examined (10 wt%, 15 wt%, and 20 wt%), at temperatures of 120°F and 200°F. Results of the study revealed that iron (III) precipitation is an obvious threat causing severe formation damage in carbonate rocks by significantly decreasing the rock permeability. Adsorption of chelating agents on limestone rocks highly depends on their concentrations. Specifically, an increase in the concentration of EDTA and DTPA at elevated temperature conditions resulted in higher adsorption. The inhibition experiments revealed that 20 wt% EDTA could significantly decrease the iron sulfide scale precipitation. Unlike the conventional testing methods for scale formation and prevention, a novel experimental setup - coreflooding during the inhibitor adsorption, formation, and inhibition of iron sulfide scale in carbonate formation was used. The main advantage of the method is the consideration of permeability alteration happening due to the scale formation. Another point is that in previous studies, various scale control chemicals and experimental approaches have been suggested for iron sulfide scale inhibition, and polymeric, phosphonate, and sulfonated co-polymeric inhibitors were used. However, the subgroup of chelating agents - aminocarboxylic acids, was used in this study.
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Gasimli, Nijat, Mohamed Mahmoud, Amjed Hassan, Muhammed Shahzad Kamal, Shirish Patil, and Ridha Al-Abdrabalnabi. "Adsorption of Aminopolycarboxylic Acids in Carbonate Formations." In SPE Annual Technical Conference and Exhibition. SPE, 2022. http://dx.doi.org/10.2118/210058-ms.
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Abstract Numerous well operations, including water injection, varying stimulation approaches, and enhanced oil recovery (EOR) techniques are implemented during the production period in order to maintain the longevity of hydrocarbon production. However, reservoir formation, production, and injection facilities are often impacted by these treatments. Well operations induce inorganic scale to form near-wellbore regions and in various production and injection structures. Consequently, the deposition of scales hinders assessing an optimum hydrocarbon production as their precipitation on formation, various surface, and downhole equipment leads to many problems, including pressure decrement, formation damage, and operational failure of subsurface equipment. As a control measure to prevent scale precipitation downhole squeeze treatment is commonly used in the petroleum industry. By applying a squeeze treatment, a scale inhibitor solution is introduced into a formation above the formation pressure, allowing the scale inhibitor to get into the deep into near-wellbore formation. Downhole squeezing allows scale inhibitors to adsorb on the internal rock surface to avoid the settling down of scale precipitates. Thus, the study of adsorption of different types of inhibitors, such as chelating agents, polymeric inhibitors, and polyphosphates on formation is becoming necessary. The study incorporated several experimental techniques, including dynamic adsorption experiments using coreflooding setup, ICP-OES (Inductively Coupled Plasma - Optical Emission Spectrometry), and ζ-potential measurements targeting evaluation of adsorption of aminopolycarboxylic acids in carbonate rocks and iron precipitation in calcite mineral. Potential precipitation of iron in varying pH environments and causing the formation of iron-containing scales was assessed through ζ-potential measurements. The findings reveal that the concentration of aminopolycarboxylic acids plays a significant role in their adsorption on carbonate rocks. The adsorption is also affected by different factors, such as the presence of salts. The results of ζ-potential measurements showed that iron (II) and iron (III) precipitation is controlled by the pH environment in calcite minerals. The treatments with 20 wt% ethylenediaminetetraacetic acid (EDTA) and diethylenetriamine pentaacetate acid (DTPA) produced the highest adsorption capacity in carbonate rock samples by inhibiting 84% and 85% of iron (III) ions, respectively. The encountered permeability damage in the adsorption tests was between 25% and 32%. Moreover, the presence of the salts considerably decreased the adsorption of EDTA and caused almost 20% more permeability reduction. Unlike the conventional testing methods for inhibitor adsorption, a novel experimental setup, coreflooding was used during the inhibitor adsorption, and scale inhibition in carbonate formation.
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Park, S., L. A. Harker, and E. G. Levin. "DEMONSTRATION OF SINGLE CHAIN UROKINASE-TYPE PLASMINOGEN ACTIVATOR (scu-PA) ON HUMAN PLATELET MEMBRANES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642906.
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A role for platelets in enhancing fibrinolysis has been suggested. To elucidate the nature of fibrinolysis-accelerating effect of human platelets, different fractions of the platelets were tested for fibrinolytic activity by the fibr:i.n plate method in the presence of plasminogen and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by fibrin autography. The platelets were washed in Tyrode’s buffer containing 0.5 ug/ml prostaglandin Ef, 1 mM ethylenediaminetetraacetic acid (EDTA), and 1 mM phenylmethylsulfonyl fluoride by albumin density gradient separation and gel filtration techniques. Platelet membranes were isolated by differential centrifugation after disruption of the platelets by ultrasonication. The sedimented platelet membranes demonstrated plasminogen activation with subsequent fibrinolysis which was completely abolished in the presence of affinity-purified antibody to high molecular weight urokinase-type plasminogen activator (u-PA). Minimal u-PA activity was observed associated with the supernatant from sonicated platelets indicating that most of the detectable u-PA was membrane-bound. Fibrin autography following SDS-PAGE of the platelet membrane fraction revealed a lysis zone at the molecular weight level of 54,000. Single chain nature of the u-PA was demonstrated by immunoblot analysis of the platelet membrane after SDS-PAGE under reducing conditions. Treatment of the platelets with 1.2 u/ml alpha-thrombin eliminated membrane-associated u-PA activity and probably resulted from the cleavage and inactivation of scu-PA. u-PA activity was quantitated by the 125I_fibrin plate method: values in the range of 0.15-0.25 mU/109 platelets were observed. Most of the activity was liberated from the platelet membrane by exposure to 3 M KC1 or 0.1 M glycine, pH 2.3, but not to 10 mM EDTA. We conclude that human platelets have scu-PA on their membranes that may play a role as a carrier of scu-PA in blood circulation. Binding studies are in progress.
Reports on the topic "Ethylenediaminetetraacetic acid (EDTA)"
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Crowley, David, Yitzhak Hadar, and Yona Chen. Rhizosphere Ecology of Plant-Beneficial Microorganisms. United States Department of Agriculture, February 2000. http://dx.doi.org/10.32747/2000.7695843.bard.
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Rhizoferrin, a siderophore produced by Rhizopus arrhizus, has been shown in previous studies to be an outstanding Fe carrier to plants. However, calculations based on stability constants and thermodynamic equilibrium lead to contradicting conclusions. In this study a kinetic approach was employed to elucidate this apparent contradiction and to determine the behavior of rhizoferrin under conditions representing soil and nutrient solutions. Stability of Fe3+ complexes in nutrient solution, rate of metal exchange with Ca, and rate of Fe extraction by the free ligand were monitored for rhizoferrin and other chelating agents by 55Fe labeling. Ferric complexes of rhizoferrin, desferri-ferrioxamine-B (DFOB), and ethylenediamine-di(o-hydroxyphenylacetic acid) (EDDHA) were found to be stable in nutrient solution at pH 7.5 for 31 days, while ferric complexes of ethylenediaminetetraacetic acid (EDTA) and mugineic acid (MA) lost 50% of the chelated Fe within 2 days. Fe-Ca exchange in Ca solutions at pH 8.7 revealed rhizoferrin to hold Fe at non-equilibrium state for 3-4 weeks at 3.3 mM Ca and for longer periods at lower Ca concentrations. EDTA lost the ferric ion at a faster rate under the same conditions. Fe extraction from freshly prepared Fe-hydroxide at pH 8.7 and with 3.2 mM Ca was slow and followed the order. DFOB > EDDHA > MA > rhizoferrin > EDTA. Based on these results we suggest that a kinetic rather than equilibrium approach should be the basis for predictions of Fe-chelates efficiency. We conclude that the non-equilibrium state of rhizoferrin is of crucial importance for its behavior as a Fe carrier to plants.