Journal articles: 'Essentials oils'

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Manion, Chelsea R., and Rebecca M. Widder. "Essentials of essential oils." American Journal of Health-System Pharmacy 74, no. 9 (May 1, 2017): e153-e162. http://dx.doi.org/10.2146/ajhp151043.

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Abstract Purpose Information to guide clinicians in educating and advising patients using or intending to use essential oils for self-administered aromatherapy or other medicinal purposes is presented. Summary The term essential oils refers to highly concentrated, aromatic oils extracted from plants by steam distillation, hydrodiffusion, or pressure. Market reports indicate strong growth in the use of essential oils in the United States in recent decades. Therapeutic claims made in the marketing of essential oils have led the Food and Drug Administration to caution a number of suppliers. Along with rapid growth in sales of essential oils to consumers there has been an increase in the amount of published evidence regarding aromatherapy and essential oils; the annual number of relevant articles indexed using Medical Subject Headings terminology has doubled since 2004. In order to help ensure proper application and safe use of essential oils as a self-care modality, healthcare professionals can benefit from a general knowledge of the terminology and foundational concepts of medicinal use of essential oils, as well as resources to facilitate evaluations of appropriateness of use. Conclusion Because of the increasing popularity of essential oils and the prevalence of essential oil–based self-care practices targeting a wide variety of ailments in the United States, healthcare professionals must be prepared to address concerns about the agents’ safety and efficacy. Proper literature evaluation requires the ability to discern the quality of an oil, the safety of administration, and the validity of its use.

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Plant, Rebecca M., Lisa Dinh, Shaara Argo, and Monica Shah. "The Essentials of Essential Oils." Advances in Pediatrics 66 (August 2019): 111–22. http://dx.doi.org/10.1016/j.yapd.2019.03.005.

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Boz, Irina, and Simona Dunca. "The Study of Essentials Oils Obtained from Thymus pannonicus L. - Microbiological Aspects." Acta Biologica Marisiensis 1, no. 1 (June 1, 2018): 53–59. http://dx.doi.org/10.2478/abmj-2018-0006.

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Abstract Essentials oils have been used over time in the food and cosmetics industry, but also in the medical and pharmaceutical industry. Environmental factors such as temperature, radiation and photoperiod play an extremely important role in the quantity and quality of volatile oils. It is also known that the vegetation stage can play an important role in the chemical composition of volatile oils. The purpose of this paper is to establish the antibacterial and antifungal activity of volatile oils of Thymus pannonicus, taking into account the ontogenetic stage in which the plants were collected, highlighting the compounds of therapeutic importance. To test the antimicrobial activity of essential oils two methods of work were used: Kirby-Bauer disc diffusion method and microplate method. The essential oils studies were tested on Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans. It was find that all essential oils tested have antimicrobial activity at all stages of development tested. The maximum antimicrobial activity has been recorded for the oils extracted from individuals collected at the anthesis stage.

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Rivaroli, Dayane Cristina, Rodolpho Martin Do Prado, Mariana Garcia Ornaghi, Camila Mottin, Tatiane Rogério Ramos, Ana Guerrero Barrado, André Mendes Jorge, and Ivanor Nunes Do Prado. "Essential Oils in the Diet of Crossbred (½ Angus vs. ½ Nellore) Bulls Finished in Feedlot on Animal Performance, Feed Efficiency and Carcass Characteristics." Journal of Agricultural Science 9, no. 10 (September 13, 2017): 205. http://dx.doi.org/10.5539/jas.v9n10p205.

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This experiment was carried out to evaluate the animal performance, feed efficiency and carcass characteristics of 27 crossbred bulls (½ Angus vs. ½ Nellore), finished in feedlot for 120 days, with the addition of distinct levels of essential oils in the diets. The experiment was conducted in a completely randomized trial and animals were distributed into three treatments: control (CON), addition of 500 mg/kg of DM/animal/day of a mix of essential oils (E500), and addition of 1000 mg/kg of DM/animal/day of a mix of essential oils (E1000). The essential oils supplemented to the diets were based on a combination of vegetal extracts from: oregano, garlic, lemon, rosemary, thymus, eucalyptus and sweet orange. The bulls had an average age of 12±2 months and weight of 243.4±6.8 kg when the trial started. The roughage and concentrate ratio was 10% and 90%, respectively. The inclusion of essentials oils in the diets did not affect final body weight, average daily gain, and feed intake, but there was a tendency for improved feed efficiency for the E500 treatment. The ADG ranged from 1.55 to 1.70. Average DMI and feed conversion were 7.07 kg/day and 4.31, respectively. Essentials oil levels did not affect the carcass characteristics. More studies are needed to elucidate the synergism of combinations of essential oils in the rumen.

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Miguel, Maria Graça, Cláudia Cruz, Leonor Faleiro, Mariana T. F. Simões, Ana Cristina Figueiredo, José G. Barroso, and Luis G. Pedro. "Foeniculum vulgare Essential Oils: Chemical Composition, Antioxidant and Antimicrobial Activities." Natural Product Communications 5, no. 2 (February 2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500231.

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The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), α-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity >50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

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Biswas, Atanu, Maria do Socorro Rocha Bastos, Roselayne Ferro Furtado, Gary Kuzniar, Veera Boddu, and H. N. Cheng. "Evaluation of the Properties of Cellulose Ester Films that Incorporate Essential Oils." International Journal of Polymer Science 2020 (May 21, 2020): 1–8. http://dx.doi.org/10.1155/2020/4620868.

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Films made from cellulose esters are often used as bio-based food packaging materials. In this work, we studied the incorporation of nine essential oils into cellulose acetate, cellulose acetate propionate, and cellulose acetate butyrate. The essential oils were derived from lime, nutmeg, eugenol, pimenta berry, rosemary, petitgrain, coffee, anise, and trans-cinnamaldehyde. In almost all cases, the addition of essential oils to cellulose ester reduced tensile strength and Young’s modulus but increased elongation at break. Thus, an essential oil acted like a plasticizer that enhanced the flexibility of the polymer. Essential oils containing limonene and pinenes (e.g., from lime and nutmeg) gave the strongest plasticizing action, whereas essentials oils containing fatty acids (e.g., from coffee) were the weakest plasticizers. The water barrier property was improved the most when essential oils were added to cellulose acetate; however, different cellulose ester/essential oil combinations showed different effects. Whereas most of the essential oils decreased the transparency of the films, eugenol, pimento berry, and anise were notable exceptions. Thus, depending on a specific application, a particular polymer/EO combination can be used to give the optimal performance.

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MILICHOVSKÝ, MILOSLAV, BŘETISLAV ČEŠEK, ONDŘEJ MIKALA, and JAN GOJNÝ. "WATER ACTIVITY RESTRICTION BY APPLICATION OF ESSENTIALS OILS." Cellulose Chemistry and Technology 53, no. 3-4 (April 26, 2019): 281–89. http://dx.doi.org/10.35812/cellulosechemtechnol.2019.53.28.

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Soran, Maria Loredana, Codruta Varodi, Simona Codruta Cobzac, and Ildiko Lung. "Essentials Oils Determination fromSatureja hortensisL. by Chromatographic Techniques." Journal of Essential Oil Bearing Plants 14, no. 6 (January 2011): 699–707. http://dx.doi.org/10.1080/0972060x.2011.10643992.

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Hussain, Khalid, and Syed Saeed-ul-Hassan . "Solubility of Essentials Oils by Non-alcoholic Agent." Journal of Medical Sciences 3, no. 3 (April 15, 2003): 220–26. http://dx.doi.org/10.3923/jms.2003.220.226.

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WIDATI, RIKHAYATI RATIH, SURANTO SURANTO, and ARTINI PANGASTUTI. "The effect of Ocimum basilicum L. essential oils toward quality of Oreochromis niloticus fillet in the cold storage." Biofarmasi Journal of Natural Product Biochemistry 4, no. 1 (February 15, 2006): 22–26. http://dx.doi.org/10.13057/biofar/f040105.

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The aims of this research was to study the effect of Ocimum basilicum L. essentials oils toward quality of Oreochromis niloticus fillet during cold storage by examining in the physical, chemical, and microbiological treatments respectivelly. The research was conducted using the complete random design of factorial consisting of 2 factors: (i) the variation of O. basilicum essential oils treatment, there were without essential oils, 0.025%, 0.05%, and 0.075%; (ii) the duration of fish storage: 0, 3, and 6 days. The observed parameters were: the physical freshness of fish, total volatile base (TVB), pH, water value, total plate count and organoleptic test. The data were analyzed by determine treatment effect toward all variable and duncanâ€™s multiple range test (DMRT) on the level 5%. The results showed that based on the physically testing control become deterioration on the third day, while fillet added with O. basilicum essential oils become deterioration in six days. Based on TVB test, the control fillet and fillet treated with 0.075% of O. basilicum essential oils become deterioration on the day of six. Fillet treated with 0.025% and 0.05% of O. basilicum essential oils still fresh in six days. The highest increasing of water value was on the control of fillet. Based on the microbiological test, the addition of O. basilicum essential oils reduced the bacterial number, fillet still fresh on the third day

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Chalchat, J. C., R. Ph. Garry, A. Michet, and E. Lecomte. "Hydroborations of some monoterpenes and sesquiterpenes from essentials oils." Flavour and Fragrance Journal 7, no. 3 (June 1992): 107–10. http://dx.doi.org/10.1002/ffj.2730070302.

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Pusceddu, Michelina, Ignazio Floris, Nicoletta P. Mangia, Alberto Angioni, and Alberto Satta. "In Vitro Activity of Several Essential Oils Extracted from Aromatic Plants against Ascosphaera apis." Veterinary Sciences 8, no. 5 (May 10, 2021): 80. http://dx.doi.org/10.3390/vetsci8050080.

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The use of natural substances such as essentials oils against bee pathogens is of great interest as an alternative to traditional methods based on synthetic compounds like antibiotics and fungicides, in order to minimize the risk of having toxic residues in hive products and to prevent the development of resistance phenomena. This study evaluated the inhibitory, fungicidal and sporicidal activity of ten essential oils extracted from aromatic plants against Ascosphaera apis, the etiological agent of chalkbrood, an invasive honey bee mycosis. The most effective essential oils were Thymus herba-barona, Thymus capitatus and Cinnamomum zeylanicum, which showed values of minimum fungicidal concentration and minimum sporicidal concentration ranging from 200 to 400 ppm. Carvacrol was the main component of Thymus capitatus and Thymus herba-barona oils, whereas cinnamic aldehyde prevailed in Cinnamomum zeylanicum oil. Further in-apiary studies will allow the evaluation of side effects on bees and residues in hive products.

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Otaifah, Y. N., A. Bouyahya, A. Talbaoui, H. Harhar, and S. El Hajjaji. "Chemical Composition of Yemeni Medicinal Plants Essentials Oils and Their Antibacterial and Antioxidant Activities." Phytothérapie 18, no. 3-4 (December 11, 2018): 195–203. http://dx.doi.org/10.3166/phyto-2018-0070.

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Medicinal plants have been used since time immemorial to fight against diseases. The biological activities of these plants may be due to the presence of bioactive compounds such as essential oils. The aim of this study was to determine the chemical composition of three Yemen medicinal plants, and e evaluate their antibacterial and antioxidant activities. The chemical composition of essential oils was determined using gas chromatography–mass spectrometry (GC–MS) analysis. The antibacterial effect was evaluated against pathogenic bacterial strains using the micro-platoon method and the antioxidant activity was estimated by DPPH (2,2-diphenyl-1-picrylhydrazyl) scanning radical assay. Essential oils (EOs) are rich in phenolic compound. Carvotanacetone is the main compound of Pulicaria jaubertii (PJ) essential oil (82.35%) and β-maaliene (64.94%) is the major compound of Plectranthus montanus Benth (PMB) essential oil, while, Tarchonanthus camphoratus (TC) essential oil possesses several main compounds such as fenchol (14.5%), α-pinene (12.43%), and α-terpineol (8.58%). Essential oils of PJ and PMB showed remarkable antibacterial effect against Staphylococcus aureus (minimum inhibitory concentration, MIC = 0.25% [v/v]), and PJEO against Escherichia coli (MIC = 0.25%[v/v]); the lowestMBC (minimum bactericidal concentration) values was obtained with PJEO against Staphylococcus aureus (CMI = 0.25% [v/v]). TCEO interestingly showed capacity while scanning the DPPH radical (6.32 mg E Trolox). These findings showed the biological potential of Yemeni aromatic medicinal plants as antibacterial and antioxidant agents. However, further investigations are needed to determine the antibacterial and antioxidant mechanisms of these oils.

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Parizotto, Carlos A., Evandro L. Dall'Oglio, Leonardo G. de Vasconcelos, Paulo T. de Sousa, Eduardo G. R. Taques Filho, and Carlos Alberto Kuhnen. "Measuring dielectric properties for microwave-assisted extraction of essential oils using single-mode and multimode reactors." RSC Advances 9, no. 10 (2019): 5259–69. http://dx.doi.org/10.1039/c8ra08727j.

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Schmidt, Erich, Jürgen Wanner, Martina Höferl, Leopold Jirovetz, Gerhard Buchbauer, Velizar Gochev, Tania Girova, Albena Stoyanova, and Margit Geissler. "Chemical Composition, Olfactory Analysis and Antibacterial Activity of Thymus vulgaris Chemotypes Geraniol, 4-Thujanol/Terpinen-4-ol, Thymol and Linalool Cultivated in Southern France." Natural Product Communications 7, no. 8 (August 2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700833.

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The essential oils of four chemotypes of Thymus vulgaris L. (Lamiaceae) were analyzed for their composition and antibacterial activity to assess their different properties. GC-MS and GC-FID analyses revealed that the essentials oils can be classified into the chemotypes thymol (41.0% thymol), geraniol (26.4% geraniol), linalool (72.5% linalool) and 4-thujanol/terpinen-4-ol (42.2% cis- and 7.3% trans-sabinene hydrate, 6.5 % terpinen-4-ol). The olfactory examination confirmed the explicit differences between these chemotypes. Furthermore, antibacterial activity was investigated against several strains of two Gram-positive ( Brochothrix thermosphacta and Staphylococcus aureus) and four Gram-negative food-borne bacteria ( Escherichia coli, Salmonella abony, Pseudomonas aeruginosa and P. fragi). All essential oil samples were demonstrated to be highly effective against Gram-positive strains, whereas the impact on Gramnegative microorganisms was significantly smaller, but still considerable. The results obtained indicate that, despite their different properties, the essential oils of selected T. vulgaris chemotypes are potent antimicrobials to be employed as useful additives in food products as well as for therapeutic applications.

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Alizadeh, Ardalan. "Essential oil composition, phenolic content, antioxidant, and antimicrobial activity of cultivated Satureja rechingeri Jamzad at different phenological stages." Zeitschrift für Naturforschung C 70, no. 3-4 (March 1, 2015): 51–58. http://dx.doi.org/10.1515/znc-2014-4121.

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Abstract Satureja rechingeri is a rare endemic and endangered species found in Iran. Its propagation, variations in essential oil and phenolic content, as well as antioxidant and antimicrobial activities at different phenological stages are reported in this study. The chemical composition of essential oils obtained by hydro-distillation from the aerial parts were determined by GC and GC-MS. A total of 47 compounds were identified in the essential oils of S. rechingeri at different phenological stages. The major components of all oils were carvacrol (83.6%–90.4%), p-cymene (0.8%–2.9%) and γ-terpinene (0.6%–2.4%). The total phenolic content and the antioxidant activity of methanolic extracts were determined with the Folin-Ciocalteau reagent and by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assay, respectively. Total phenols varied from 35.5 to 37.5 mg gallic acid equivalents/g dry weight (dw), and IC50 values in the radical scavenging assay ranged from 46.2 to 50.2 mg/mL, while those in the FRAP assay were between 49.6 and 52.5 μM quercetin equivalents/g dw. By the disc diffusion method and by determination of the minimum inhibitory concentrations (MIC), the essentials oils of the various phenological stages were found to have high activities against four medically important pathogens.

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Rasekh, Mansour, Hamed Karami, Alphus Dan Wilson, and Marek Gancarz. "Performance Analysis of MAU-9 Electronic-Nose MOS Sensor Array Components and ANN Classification Methods for Discrimination of Herb and Fruit Essential Oils." Chemosensors 9, no. 9 (August 31, 2021): 243. http://dx.doi.org/10.3390/chemosensors9090243.

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The recent development of MAU-9 electronic sensory methods, based on artificial olfaction detection of volatile emissions using an experimental metal oxide semiconductor (MOS)-type electronic-nose (e-nose) device, have provided novel means for the effective discovery of adulterated and counterfeit essential oil-based plant products sold in worldwide commercial markets. These new methods have the potential of facilitating enforcement of regulatory quality assurance (QA) for authentication of plant product genuineness and quality through rapid evaluation by volatile (aroma) emissions. The MAU-9 e-nose system was further evaluated using performance-analysis methods to determine ways for improving on overall system operation and effectiveness in discriminating and classifying volatile essential oils derived from fruit and herbal edible plants. Individual MOS-sensor components in the e-nose sensor array were performance tested for their effectiveness in contributing to discriminations of volatile organic compounds (VOCs) analyzed in headspace from purified essential oils using artificial neural network (ANN) classification. Two additional statistical data-analysis methods, including principal regression (PR) and partial least squares (PLS), were also compared. All statistical methods tested effectively classified essential oils with high accuracy. Aroma classification with PLS method using 2 optimal MOS sensors yielded much higher accuracy than using all nine sensors. The accuracy of 2-group and 6-group classifications of essentials oils by ANN was 100% and 98.9%, respectively.

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da Costa, Jamile S., Ellen de Nazaré S. da Cruz, William N. Setzer, Joyce Kelly do R. da Silva, José Guilherme S. Maia, and Pablo Luis B. Figueiredo. "Essentials Oils from Brazilian Eugenia and Syzygium Species and Their Biological Activities." Biomolecules 10, no. 8 (August 6, 2020): 1155. http://dx.doi.org/10.3390/biom10081155.

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The Eugenia and Syzygium genera include approximately 1000 and 1800 species, respectively, and both belong to the Myrtaceae. Their species present economic and medicinal importance and pharmacological properties. Due to their chemical diversity and biological activity, we are reporting the essential oils of 48 species of these two genera, which grow in South America and found mainly in Brazil. Chemically, a total of 127 oil samples have been described and displayed a higher intraspecific and interspecific diversity for both Eugenia spp. and Syzygium spp., according to the site of collection or seasonality. The main volatile compounds were sesquiterpene hydrocarbons and oxygenated sesquiterpenes, mainly with caryophyllane and germacrane skeletons and monoterpenes of mostly the pinane type. The oils presented many biological activities, especially antimicrobial (antifungal and antibacterial), anticholinesterase, anticancer (breast, gastric, melanoma, prostate), antiprotozoal (Leishmania spp.), antioxidant, acaricidal, antinociceptive and anti-inflammatory. These studies can contribute to the rational and economic exploration of Eugenia and Syzygium species once they have been identified as potent natural and alternative sources to the production of new herbal medicines.

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Ganesan, Jegannathan, Vighnesh Adikesavan, and Srimathi Devi Jegannathan. "Anti – Microbial effect of three essential oils in treating Upper respiratory tract Infection via vapours generated by Pressurized steam chamber (PSC)." International Journal of Ayurvedic Medicine 12, no. 4 (December 31, 2021): 943–47. http://dx.doi.org/10.47552/ijam.v12i4.2191.

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Disinfection tunnel (DT) or sanitization tunnel used for disinfecting the persons by spraying with appropriately atomized virucide spray, Direct inhalation or spraying of disinfectants on people with chemical element and different toxicant chemicals may lead to eye and skin irritation and cause numerous allergic disorders. So this obstacle was overcome by Ideal flow control private limited by designing the Pressurized Steam Chamber (PSC) in which Natural oils was mixed in an emulsifier solution, and induced along with steam through multiple nozzles within the chamber, so that persons could get disinfected the entire body. Anti-microbial efficacy of our three essentials oils were determined by standard method, ISO 4833-1:2013 by collecting sample through walk in/walk out chamber protocol. Our present study report of MSME (ministry of micro, small and medium enterprises) and SGS Chennai, reveals that in phytotherapeutic oils initial microbial load are found to be <1 CFU / mL, in emulsifier and in formulation (water + emulsifier + natural oils) the presence of bacterial colonies found to be 470000 CFU / mL and 20000000 CFU / mL whereas, the distillate collected from essential oils at 65̊ c shows the absence of microbial load. Further swab analysis report of 8 individuals states that samples (hand swab and surface swab) collected for the estimation of Total plate Count showed that there is a reduction in microbial load when exposed to the Steam generated by Pressurized Steam Chamber (PSC) at both the time intervals, 20 and 40 seconds. These findings confirm that three essential phytotherapeutic oils combined with steam have some potent activity against emerging disease.

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Zeman, Ladislav, Petr Mareš, and Michal Večerek. "The effect of anethole containing essentials oils on nutrients digestibility of pigs." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 57, no. 1 (2009): 171–80. http://dx.doi.org/10.11118/actaun200957010171.

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The plant additives and their functional components can selectively influence the intestinal microorganism growth in positive or negative direction. If the growth promotion relates with positive microorganisms and growth elimination is connected with pathogens the results is nutrients utilization improvement, stimulation of immunologic system or positive influence of intermedial metabolism. Anethole is one of these important plant metabolites. There are described following positive effects of anethole in human medicine: vasorelaxant, antithrombotic, releasing of heart function, fytoestrogenic (it mean improving of milk secretion, menstruation, promotion of menses, birth improvement, men’s hormonal changes improving, sexual libido improving), antioxidative, antifungal, improvement of derma permeability, antihelmintic, insecticidal, yeast elimination, antibacterial, antipyretic.In our work the effect of anise and fennel essential oils on nutrients utilization in pig experiment was evaluated. The trial was organized in accredited experimental stable Žabčice of Mendel University of Agriculture and Forestry Brno. The high level efficiency of experimental animals is mentioned through general parameters (average daily gain, feed intake and feed conversion). The results of nutrients utilization rate show slightly higher digestibility of nutrients in treatment with anise oil, this improvement is not higher then 1.0 %. We can see also improvement of nitrogen retention in body mass on level of 5.6 % (anise treatment compared with control group). There is low variability between experimental animals (except nitrogen retention coefficient) but we can not see any statistical significance. On base of these results we can say the used phytogenic additives do not affect negatively the nutrient utilization in used concentration (0.1 % of essential oil in feed mixture) and are fully eligible for animal nutrition. These results are also supported by few research papers connected with similar topic.

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KULPA, Danuta, Aneta WESOŁOWSKA, and Paula JADCZAK. "Micropropagation and Composition of Essentials Oils in Garden Thyme (Thymus vulgaris L.)." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46, no. 2 (February 21, 2018): 525–32. http://dx.doi.org/10.15835/nbha46211020.

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Thymus vulgaris L. is an important aromatic plant, because of the synthesis and production of its essential oils for the pharmaceutical and cosmetic industries. In this study, we developed a micropropagation protocol for T. vulgaris ‘Słoneczko’ and evaluated the potential of micropropagated plants for essential oil production with industrial application. The seeds were soaked for 10 min in 10% sodium hypochlorite (NaOCl) solution. Then, each seed was put into a 20 ml test tube filled with 5ml of Murashige and Skoog (MS) medium. Half of the cultures were subjected to light intensity which was maintained at 40 µEm−2s−1, and the other half was cultured in the dark. Shoot explants were multiplied in vitro using MS medium supplemented with BAP, 2iP or KIN. The results obtained indicate that the cytokinin which had the most positive impact on plant development at the multiplication stage was 5 mg dm−3 2iP. Single-node fragments of shoots cultured on MS medium without plant growth regulators were transferred to MS medium supplemented with IAA, IBA and NAA at concentrations of 0.2, 0.5, 1 and 2 mgdm−3. The best rooting of shoots was obtained on MS medium supplemented with 2 mgdm−3 IBA. The essential oils obtained by hydrodistillation in Deryng and Clevenger apparatus from in vitro shoot cultures of T. vulgaris L. were analysed using gas chromatography-mass spectrometry (GC-MS). Analysis revealed the presence of 54 components represented mainly by oxygenated monoterpenes (56.81-57.28%) and monoterpene hydrocarbons (31.90-33.72%). Among identified constituents, the most abundant were thymol (33.37-34.05%), γ-terpinene (11.62-11.91%), p-cymene (9.81-10.07%), carvacrol (5.63-5.96%), carvacrol methyl ether (3.86-3.87%) and linalool (3.16-3.36%).

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Sieniawska, Elwira, Łukasz Świątek, Michał Wota, Barbara Rajtar, and Małgorzata Polz-Dacewicz. "Microemulsions of essentials oils – Increase of solubility and antioxidant activity or cytotoxicity?" Food and Chemical Toxicology 129 (July 2019): 115–24. http://dx.doi.org/10.1016/j.fct.2019.04.038.

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Mulyanti, Sri, Dewi Sodja Laela, Euis Julaeha, Anne Agustina Suwargiani, and Dudi Aripin. "Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro." Padjadjaran Journal of Dentistry 32, no. 1 (March 31, 2020): 39. http://dx.doi.org/10.24198/pjd.vol32no1.25486.

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Introduction: Essentials oil of lime (Citrus aurantifolia) peel has high activity in inhibiting the growth of Streptococcus mutans bacteria. The objective of this research was to determine the formulation of mouth rinse from the essential oils of lime (C. aurantifolia) with the highest inhibitory and on the growth of S. mutans. Methods: This research was an experimental in-vitro study. The sample used was the lime (C. aurantifolia) peel waste peel using hydrodistillation technique; testing of the essential oil quality was performed using 100°C distilled water for 3 hours; organoleptic test and physical mouth rinse formula test was also conducted. Mouth rinse formulations were made from the essential oils in the concentrations of 1, 2, 4, 8, 10, and 20%. Mouth rinse activity was tested with optical density. Minimum inhibitory concentration was determined with microdilution. Results: Essential oil mouth rinse formulation showed antibacterial activity at the concentrations of 2, 4, 8, 10, and 20% (formulations I-IV); the recommended mouth rinse concentration was 4% (formulation III) due to its highest antibacterial. Formulation IV showed the highest minimum inhibitory concentration value of 5.2 mg/mL (0.52%) and the safe minimum bactericidal concentration of 0.4 mg/mL (1.04). Conclusion: Mouth rinse formulation from essential oils of lime (C. aurantifolia) peel begins to show inhibition of the growth of S. mutans bacteria at the concentration of 2% essential oils (formulation II), with inhibition against the growth of S. mutans is 7.08. The formula shows a minimum inhibitory concentration (MIC) value and a minimum bactericidal concentration (MBC) of 5.2 mg/mL (0.52%) and 10.4 mg/mL (1.04), respectively.

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N’goran, Koua Serge Beranger, Brahima Camara, Aya Carine N’guessan, Nahawa Kone, Marie-Solange Tiebre, Djakalia Ouattara, and Severin Ake. "In vitro activities of fungicides based of plants essentials oils (NECO, ASTOUN and FERCA) and phosphorous acid on Phytophthora katsurae (Pythiaceae), causal agent of the premature nut fall and the heart rot of the coconut tree, in Côte d’Iv." International Journal of Biological and Chemical Sciences 15, no. 5 (January 23, 2022): 1968–78. http://dx.doi.org/10.4314/ijbcs.v15i5.22.

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The present study aimed at evaluating the antifungal activities of NECO, ASTOUN and FERCA fungicides based of plants essentials oils against Phytophthora katsurae a major constraint in coconut plantations in Côte d'Ivoire. In vitro, the inhibition properties of these fungicides was evaluated at different concentrations 50, 100, 250, 500, 1000, 1500, 2000 and 2500 ppm and compared to a synthetic fungicide (phosphorous acid) at 1, 5, 10, 25, 50, 100 and 150 ppm on the radial growth of the mycelium of an isolated pathogen from a diseased nut. The mycelial growth was measured every day during 10 days in the Petri dishes. The results showed inhibition rates ranging from 1.48% to 100%. The biopesticides NECO and ASTOUN were effective against Phytophthora katsurae at respectively 149.14 and 272.38 ppm compared to phosphorous acid. NECO was fungitoxic at 2500 ppm and fungistatic at 1500 and 2000 ppm. Phosphorous acid was simply fungicidal at 150 ppm. Based on these results, these fungicides based of plants essentials oils could be used as biological control of Pnhytophthora katsurae in coconut plantations in Côte d'Ivoire.

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Mnayer, Dima, Anne-Sylvie Fabiano-Tixier, Emmanuel Petitcolas, Tayssir Hamieh, Nancy Nehme, Christine Ferrant, Xavier Fernandez, and Farid Chemat. "Chemical Composition, Antibacterial and Antioxidant Activities of Six Essentials Oils from the Alliaceae Family." Molecules 19, no. 12 (December 1, 2014): 20034–53. http://dx.doi.org/10.3390/molecules191220034.

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Soran, Maria Loredana, Simona Codruta Cobzac, Codruta Varodi, Ildiko Lung, Emanoil Surducan, and Vasile Surducan. "The extraction and chromatographic determination of the essentials oils fromOcimum basilicumL. by different techniques." Journal of Physics: Conference Series 182 (August 1, 2009): 012016. http://dx.doi.org/10.1088/1742-6596/182/1/012016.

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Viuda-Martos, M., M. A. Mohamady, J. Fernández-López, K. A. Abd ElRazik, E. A. Omer, J. A. Pérez-Alvarez, and E. Sendra. "In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants." Food Control 22, no. 11 (November 2011): 1715–22. http://dx.doi.org/10.1016/j.foodcont.2011.04.003.

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Carvalhinho, Sara, Ana Margarida Costa, Ana Cláudia Coelho, Eugénio Martins, and Ana Sampaio. "Susceptibilities of Candida albicans Mouth Isolates to Antifungal Agents, Essentials Oils and Mouth Rinses." Mycopathologia 174, no. 1 (January 14, 2012): 69–76. http://dx.doi.org/10.1007/s11046-012-9520-4.

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Bilel, Hallouma, Lamia Boubakri, Fethi Zagrouba, and Naceur Hamdi. "Chemical composition, antimicrobial and antioxidant activities of the essentials oils from flowers of Salvia sharifii." European Journal of Chemistry 6, no. 3 (September 30, 2015): 301–4. http://dx.doi.org/10.5155/eurjchem.6.3.301-304.1264.

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Yarou, Boni Barthélémy, Thomas Bawin, Antoine Boullis, Stéphanie Heukin, Georges Lognay, François Jean Verheggen, and Frédéric Francis. "Oviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae)." Environmental Science and Pollution Research 25, no. 30 (August 7, 2017): 29880–88. http://dx.doi.org/10.1007/s11356-017-9795-6.

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Primasari, Putri Intan, Dwi Murtiastutik, Pepy Dwi Endraswari, Cita Rosita Sigit Prakoeswa, and Evy Ervianti. "Comparison of In Vitro Testing Antifungal Activity between Rosemary Essentials Oil and Fluconazol on Candida species Isolate from HIV/AIDS Patients with Candidiasis Oral." Berkala Ilmu Kesehatan Kulit dan Kelamin 32, no. 3 (November 30, 2020): 182. http://dx.doi.org/10.20473/bikk.v32.3.2020.182-188.

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Background: The most common opportunistic infection obtained in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) is Oral Candidiasis (OC). Currently widely reported about increased fluconazole resistance, as a therapy that is often used in patients with HIV/AIDS with OC. For this reason, effective antifungal agents are needed as alternative therapies. Several studies have reported some antifungal activity from rosemary essential oils. Purpose: The aim of this study is to evaluate the antifungal activity between rosemary essential oils and fluconazole against the isolates used by Candida sp. taken from HIV / AIDS patients with OC. Methods: This research was an experimental laboratory study with a post-test only design of 40 Candida sp. isolates from HIV / AIDS patients with OC in the Outpatient Unit and Inpatient Installation of the Infectious Disease Intermediate Care Unit (UPIPI) Dr. Soetomo General Academic Teaching Hospital, Surabaya. Examination of antifungal activity using conventional test methods with disk diffusion. Result: Disk diffusion test result revealed average inhibition zone of rosemary essential oil in Candida albicans 1,75±3,64 mm and Candida non-albicans 1,5±3,08 mm, while the average inhibition zone of fluconazole in Candida albicans 17,9±8,62 mm and Candida non-albicans 4,85±7,9 mm. There was significant difference (p <0.001) between the inhibition zone formed in Candida albicans by fluconazole and rosemary essential oil, but no significant difference of inhibition zone formed in Candida non-albicans. Conclusion: Antifungal activity fluconazole has greater than Rosemary essential oils in Candida albicans.

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Rabib, Halima, Sophia Zougagh, Mohamed Hsain, Wadi Badri, and Tayeb Koussa. "GC/MS Analysis and Antibacterial Activity of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters." Mediterranean Journal of Chemistry 8, no. 4 (June 4, 2019): 302–7. http://dx.doi.org/10.13171/mjc84190706041316hr.

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The quality and the variability of the chemical composition and antimicrobial properties of essentials oils isolated from Tetraclinis articulata (Vahl) leaves, which were studied from two different regions that are ecologically various. The average yield of essential oil is 0,56 % for the Ras Elma Tazekka region (mountain) and 0,36% for the Debdou region (plain). An analysis made by GC and GC/ MS identified 22 components in Tetraclinis articulata leaves essential oil in the mountain area and 29 components in the plain area. These major components represent the variability of quantity. The percentages following respectively by the two areas are: Bornyl acetate (34,84%; 32,55%), α-Pinene (11,41%; 18,83%), Camphor (11,24 %; 11,31%), Limonene (11,94 %; 8%) and Borneol (8,35%; 7,79%). The antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli which has been inhibited from the 50μg / mL concentration for the Debdou region (Plain) and 25μg / mL for that of the Ras Elma Tazekka region (Mountain).

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Kone, A. P., D. Cinq-Mars, Y. Desjardins, F. Guay, A. Gosselin, and L. Saucier. "Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat." World Rabbit Science 24, no. 2 (June 29, 2016): 107. http://dx.doi.org/10.4995/wrs.2016.3665.

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The effects of dietary supplementation of onion, cranberry, strawberry and essentials oils on meat quality were analysed. Five groups of 48 Grimaud female weaned rabbits received the supplemented or the control ration; the experimental unit was a cage of 6 rabbits. Each experimental diet contained 10 ppm of added active ingredients. Rabbits were fed with the experimental diets for 4 wk before determining slaughter and carcass traits and determining the pH at 1 and 24 h post mortem (pHu) of the Longissimus dorsi (LD) and the Biceps femoris (BF) muscle, left and right, respectively. Cooking loss, drip loss and L*, a* and b* color parameters were obtained of the right LD and for ground meat and antioxidant status (TBARS, DNPH, Folin Ciocalteu). Only the pHu of the LD muscle for the strawberry supplemented group was significantly lower when compared to the control group (P=0.04). However, we note that for the pH of the LD, the average was less than 6 for the meat of animals who received a diet enriched in polyphenols, compared to the control group. Plant extract supplementation did not influence meat quality traits, growth performance or oxidative stability. But under aerobic and anaerobic conditions, our results indicate that diet supplementation with extracts rich in polyphenols, especially with essential oils, had a small bot sporadic positive effect in reducing bacterial microflora compared to the control group (P<0.05). In conclusion, plant extracts and essential oils can be used in a rabbit diet without adverse effects on performance and meat quality traits. This effect could be optimized by investigating higher doses.

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Ergin, Kubra Nalkiran, Songul Karakaya, Gamze Göger, Oksana Sytar, Betul Demirci, and Hayri Duman. "Anatomical and Phytochemical Characteristics of Different Parts of Hypericum scabrum L. Extracts, Essential Oils, and Their Antimicrobial Potential." Molecules 27, no. 4 (February 11, 2022): 1228. http://dx.doi.org/10.3390/molecules27041228.

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Hypericum (Hypericaceae) is a genus that comprises a high number of species around the world. In this study, the roots, aerial parts, flowers, fruits, and aerial parts with flowers from Hypericum scabrum were macerated separately by methanol and water and then fractionated by different solvents of, such as ethyl acetate, n-hexane, butanol, dichloromethane, aqueous residue sub-extracts, and ethnobotanical use. All the extracts, sub-extracts and essential oils of H. scabrum were investigated for the first time in detail for their antimicrobial, total phenolics, and antioxidant activities. Anatomical structures of the root, stem, leaf, upper and lower leaf surface, stamen, sepal, and petal of H. scabrum were examined. The biochemical layout of essential oils was determined by GC and GC/MS. The antioxidant activity was determined by free radical scavenging activity (by DPPH). Antimicrobial activity was applied against Candida albicans ATCC 10231, Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 19659, and C. tropicalis ATCC 750 using microdilution methods. The essentials of the aerial parts, flower, and fruit are characterized by the presence of monoterpene hydrocarbons, whereas roots oil include alkanes. The GC-FID and GC-MS analysis showed that major components of roots, aerial parts, flowers, and fruits oils were undecane (66.1%); α-pinene (17.5%), γ-terpinene (17.4%), and α-thujene (16.9%); α-pinene (55.6%), α-thujene (10.9%), and γ-terpinene (7.7%); α-pinene (85.2%), respectively. The aerial part sub-extracts indicated a greater level of total phenolics and antioxidant potential. The n-hexane sub-extracts (from aerial part, flower, and aerial part with flower) showed the best activity against B. subtilis, with 39.06 µg/mL MIC value. The presented research work indicates that H. scabrum can be a novel promising resource of natural antioxidant and antimicrobial compounds.

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Hilali, Miloudi, and Zoubida Charrouf. "Essentials Oils and the Phenolic Compositions of the Fruit Pulp of the Argan Fruit at Morocco." Journal of Agronomy 19, no. 3 (June 15, 2020): 156–61. http://dx.doi.org/10.3923/ja.2020.156.161.

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Gabbanini, S., E. Lucchi, M. Carli, E. Berlini, A. Minghetti, and L. Valgimigli. "In vitro evaluation of the permeation through reconstructed human epidermis of essentials oils from cosmetic formulations." Journal of Pharmaceutical and Biomedical Analysis 50, no. 3 (October 2009): 370–76. http://dx.doi.org/10.1016/j.jpba.2009.05.018.

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Cerceau, Cristiane I., Luiz C. A. Barbosa, Claudinei A. Filomeno, Elson S. Alvarenga, Antônio J. Demuner, and Paulo H. Fidencio. "An optimized and validated 1H NMR method for the quantification of α-pinene in essentials oils." Talanta 150 (April 2016): 97–103. http://dx.doi.org/10.1016/j.talanta.2015.10.087.

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Castellino, Giuseppa, Francisco Mesa, Francesco Cappello, Cristina Benavides-Reyes, Giuseppe Antonio Malfa, Inmaculada Cabello, and Antonio Magan-Fernandez. "Effects of Essential Oils and Selected Compounds from Lamiaceae Family as Adjutants on the Treatment of Subjects with Periodontitis and Cardiovascular Risk." Applied Sciences 11, no. 20 (October 14, 2021): 9563. http://dx.doi.org/10.3390/app11209563.

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Essential oils from different plant species were found to contain different compounds exhibiting anti-inflammatory effects with the potential to be a valid alternative to conventional chemotherapy that is limited in long-term use due to its serious side effects. Generally, the first mechanism by which an organism counteracts injurious stimuli is inflammation, which is considered a part of the innate immune system. Periodontitis is an infectious and inflammatory disease caused by a dysbiosis in the subgingival microbiome that triggers an exacerbated immune response of the host. The immune–inflammatory component leads to the destruction of gingival and alveolar bone tissue. The main anti-inflammation strategies negatively modulate the inflammatory pathways and the involvement of inflammatory mediators by interfering with the gene’s expression or on the activity of some enzymes and so affecting the release of proinflammatory cytokines. These effects are a possible target from an effective and safe approach, suing plant-derived anti-inflammatory agents. The aim of the present review is to summarize the current evidence about the effects of essentials oils from derived from plants of the Lamiaceae family as complementary agents for the treatment of subjects with periodontitis and their possible effect on the cardiovascular risk of these patients.

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Sliba, Bianca Maria Borges, Mariana Martins Juglair, Fernanda Alves, Marilisa Carneiro Leão Gabardo, Carolina Dea Bruzamolin, and João Armando Brancher. "The antimicrobial activity of essentials oils of thyme, oregano, copaiba, tea tree, and frankincense against Enterococcus faecallis / Análise da atividade antimicrobiana dos óleos essenciais de tomilho, orégano, copaíba, melaleuca, e olíbano contra Enterococcus faecallis." Brazilian Journal of Development 8, no. 2 (February 4, 2022): 9079–91. http://dx.doi.org/10.34117/bjdv8n2-045.

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Oral diseases are among the problems that most affect the quality of life of people in the world. Traditional methods for drug treatment of these diseases are almost always expensive and, in addition, they cause side effects and can promote an increase in bacterial resistance. Thus, the demand for plant-derived products, such as herbal, floral and essential oils, has been growing every day, favoring the development of new therapeutic products that can replace or complement traditional therapeutic treatment. Among the natural products that have been gaining space, essential oils (EOs) stand out. Therefore, the aim of this study was to evaluate the role of thyme, oregano, copaiba, tea tree and frankincense EOs in inhibiting the growth of Enterococcus faecalis bacteria. The oil diffusion technique in agar was performed to evaluate the efficiency of the EOs, at different concentrations: 25%, 50%, 75% and 100%. The EOs that showed the greatest inhibition halo were, in decreasing order: Thyme Oil (5.0 mm), Tea tree Oil (4.70 mm) and Oregano Oil (3.75mm) in the concentration of 100%, with results similar to 0.12% chlorhexidine (positive control), whose inhibition halo was 3 mm. Copaiba and Frankincense oils were not efficient to inhibit bacterial growth at concentrations of 25, 50 and 75%. The results obtained in this research demonstrated that thyme, tea tree and oregano oils inhibited the growth of Enterococcus faecalis bacteria.

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Salifou, Soumanou, Houénagnon Marcel Aristide Houngnimassoun, Ignace Ogoudananan Dotche, Sabbas Attindehou, and Sahidou Salifou. "Bioactivity of essentials oils of Hyptis suaveolens (L.) on the reproductive parameters of the Rhipicephalus (Boophilus) microplus." Journal of Phytopharmacology 9, no. 2 (April 28, 2020): 125–29. http://dx.doi.org/10.31254/phyto.2020.9209.

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Zhigzhitzhapova, Svetlana V., Tuyana E. Randalova, Larisa D. Radnaeva, Elena P. Dylenova, Shilong Chen, and Faqi Zhang. "Chemical Composition of Essentials Oils of Artemisia frigida Willd. (Asteraceae) Grown in the North and Central Asia." Journal of Essential Oil Bearing Plants 20, no. 4 (July 4, 2017): 915–26. http://dx.doi.org/10.1080/0972060x.2017.1377113.

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Fitsiou, Eleni, and Aglaia Pappa. "Anticancer Activity of Essential Oils and Other Extracts from Aromatic Plants Grown in Greece." Antioxidants 8, no. 8 (August 7, 2019): 290. http://dx.doi.org/10.3390/antiox8080290.

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Aromatic plants have a long and significant history in the traditional medicine of many countries. Nowadays, there is an increasing interest in investigating the biological properties of aromatic plant extracts mainly due to their diversity, high availability, and low toxicity. Greece is abundant in aromatic plants, which can be attributed to the country’s geographical position, the morphology of its landscape, and its numerous mountainous and insular areas. In the past 15 years, a number of aromatic plant extracts of Greek origin have been studied for their bioactivities, including their antiproliferative potential against different types of cancer. Although the pharmacological activities of specific species of Greek origin have been reviewed before, no gathered information on explicitly Greek species exist. In this review, we summarize existing data on the antiproliferative activity of extracts isolated from Greek aromatic plants and discuss their molecular mode(s) of action, where available, in order to identify promising extracts for future research and link chemical constituents responsible for their activity. We conclude that essentials oils are the most frequently studied plant extracts exhibiting high diversity in their composition and anticancer potential, but also other extracts appear to be worthy of further investigation for cancer chemoprevention.

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Arya, Anita, S. K. Mendiratta, R. K. Agarwal, S. K. Bharti, and Pramila Umarao. "Antimicrobial profile and organoleptic acceptability of some essentials oils and their blends in hurdle treated chicken meat spread." International Journal of Current Microbiology and Applied Sciences 8, no. 09 (September 10, 2019): 2162–77. http://dx.doi.org/10.20546/ijcmas.2019.809.250.

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Selmi, Slimen, Kais Rtibi, Dhekra Grami, Azhar Hajri, Karim Hosni, Lamjed Marzouki, and Hichem Sebai. "Antioxidant properties of Artemisia herba-alba and Eucalyptus camaldulensis essentials oils on malathion-induced reproductive damage in rat." RSC Advances 6, no. 112 (2016): 110661–73. http://dx.doi.org/10.1039/c6ra18268b.

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Uchenna, Amadi P., Ogunka-Nnoka U. Charity, and Abbey Bene. "Properties of Oils From Plantain Pseudostem Biotransformed Using Crude Local Enzyme Sources: A Comparison of Poultry Feed Oil." Recent Patents on Food, Nutrition & Agriculture 10, no. 2 (September 18, 2019): 140–51. http://dx.doi.org/10.2174/2212798410666181217141311.

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Background: Plantain Pseudostem (PPS) wastes were biotransformed by applying Simultaneous Saccharification and Fermentation (SSF) using excised snail digestive juice and yeast slurry, and their oil properties compared with oils from commercially sold poultry feeds (PF). Patents suggesting the inclusion of certain additives (US20090226558A1), spices (US5741508A), cysteamine (US4711897A), and dextrin (US6326051B1) in animal diets are regarded as expensive, thus, requiring cheaper and readily available sources of growth. Methods: The analysis of their free radical scavenging potentials was carried by spectrophotometry, while fatty acids, volatile fatty acids, essentials oils, and phytosterols were determined by chromatography. Results: After biotransformation, the melting point, specific gravity, acid, and peroxide values of the oils from SSF-PPS were significantly lower than those of PF, and showed elevations of C6:0- C18:1(trans-9) fatty acids, palmitic, stearic, γ-Linolenic, α-linolenic, behenic, and lignoceric acids. Camphene, β-phelandrene, eugenol, β-elemene, bicyclogermacrene, guaiol, tetradecanoic acid, and hexadecanoic acid levels decreased when PPS was biotransformed. Lactic (1575.75 mg/100g), acetic (1234.26 mg/100g), propionic (845.74 mg/100g), and n-butyric (68.56 mg/100g) acids were the predominant Volatile Fatty Acids (VFAs) in the SSF-PPS oils, which were higher than those found in the PF oil while PF oil contained higher campesterol, Stigmasterol, and 5-avesmasterol. The 2,2- azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 1,1-diphenyl-2- picrylhydrazyl (DPPH), superoxide, and nitric oxide radical scavenging potentials of PPS oil at high concentrations after biotransformation were equivalent to the standards and the PF oil. Conclusion: his study has shown that biotransformation involving snail digestive juice and yeasts extensively improves the oil qualities of agricultural residues sufficient enough for poultry nutrition.

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Ngongang, Dimitri Tchami, Ascension Maximilienne Nyegue, Del Florence Esther Ndedi, and Xavier François Etoa. "Effect of Four Essential Oils on Cells Release Membrane and Biofilm Formation of Clinical Bacterial Isolated From Oral Infection." Journal of Drug Delivery and Therapeutics 9, no. 6 (November 15, 2019): 28–35. http://dx.doi.org/10.22270/jddt.v9i6.3600.

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Background: Bacterial biofilms forming are current resistant bacterial form to the treatment of oral diseases that colonizes in the gingival and sub-gingival regions of the mouth. The present study aims to screen the anti-biofilm potential and evaluate the effect of four essential oils on cells release membrane. Methods: Seven type isolate bacteria obtained during previous work were screen to select those who had ability to form biofilm using Congo Red Agar method, tube method and crystal violet method. The inhibitory parameter of biofilm forming was determine using microtiter plate method. The effect of essential oil on cell membrane release of each selected bacterial was put in evidence by measuring cellular material that absorb at 260 nm and 280 nm after 0 min, 30 min and 60 min of exposure and confirm by measuring DNA, RNA and proteins release by treated cells on extracellular medium using Nanodrop 1000 spectrophotometer. Results: The crystal violet method shows twelve (12) strong, five (05) moderate and five (05) weak biofilm forming bacteria. The anti-biofilm activity against the oral bacteria who shown that most of essentials oils have activity on different biofilm formation and the MICs ranged from 0.31 mg/mL to 1.25 mg/mL. Concentration of intracellular material released in extracellular medium ranged from 186,56 ± 2,35 ng/µL to 766,6 ± 2,84 ng/µL for DNA, 158,06 ± 1,87 ng/µL to 628,53 ± 2,05 ng/µL for RNA and 695,9 ± 2,11ng/µL to 1125,23 ± 2,15 ng/µL for proteins. Conclusion: This study demonstrates that the selected EOs have a significant anti-biofilm activity, acting on the cell surface and causing the disruption of the bacterial membrane. These EOs are interesting alternative to conventional antimicrobials for the control of oral microorganisms. Keywords: Anti-biofilm activity, Biofilm, oral diseases

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Chillet, Marc, Angélique Hoareau, Mathilde Hoarau, and Jérome Minier. "Potential Use of Essentials Oils to Control Fruitlet Core Rot (FCR) in Pineapple (Queen Victoria Variety) in Reunion Island." American Journal of Plant Sciences 11, no. 11 (2020): 1671–81. http://dx.doi.org/10.4236/ajps.2020.1111119.

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Ocaña-Fuentes, A., E. Arranz-Gutiérrez, F. J. Señorans, and G. Reglero. "Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: Anti-inflammatory properties based on cytokine response on THP-1 macrophages." Food and Chemical Toxicology 48, no. 6 (June 2010): 1568–75. http://dx.doi.org/10.1016/j.fct.2010.03.026.

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Mahnashi, Mater H., Yahya S. Alqahtani, Bandar A. Alyami, Ali O. Alqarni, Muhammad Ayaz, Mehreen Ghufran, Farhat Ullah, et al. "Phytochemical Analysis, α-Glucosidase and Amylase Inhibitory, and Molecular Docking Studies on Persicaria hydropiper L. Leaves Essential Oils." Evidence-Based Complementary and Alternative Medicine 2022 (January 19, 2022): 1–11. http://dx.doi.org/10.1155/2022/7924171.

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Objective. Medicinal plants and essentials oils are well known for diverse biological activities including antidiabetic potential. This study was designed to isolate essential oils from the leaves of Persicaria hydropiper L. (P. hydropiper), perform its phytochemical analysis, and explore its in vitro antidiabetic effects. Materials and Methods. P. hydropiper leaves essential oils (Ph.Los) were extracted using a hydrodistillation apparatus and were subjected to phytochemical analysis using the gas chromatography mass spectrometry (GC-MS) technique. Ph.Lo was tested against two vital enzymes including α-glucosidase and α-amylase which are important targets in type-2 diabetes. The identified compounds were tested using in silico approaches for their binding affinities against the enzyme targets using MOE-Dock software. Results. GC-MS analysis revealed the presence of 141 compounds among which dihydro-alpha-ionone, cis-geranylacetone, α-bulnesene, nerolidol, β-caryophyllene epoxide, and decahydronaphthalene were the most abundant compounds. Ph.Lo exhibited considerable inhibitory potential against α-glucosidase enzyme with 70% inhibition at 1000 μg mL−1 which was the highest tested concentration. The inhibitory activity of positive control acarbose was 77.30 ± 0.61% at the same tested concentration. Ph.Lo and acarbose exhibited IC50 of 170 and 18 µg mL−1 correspondingly. Furthermore, dose-dependent inhibitions were observed for Ph.Lo against α-amylase enzyme with an IC50 of 890 μg mL−1. The top-ranked docking conformation was observed for β-caryophyllene epoxide with a docking score of -8.3182 against α-glucosidase, and it has established seven hydrogen bonds and one H-pi interaction at the active site residues (Phe 177, Glu 276, Arg 312, Asp 349, Gln 350, Asp 408, and Arg 439). Majority of the identified compounds fit well in the binding pocket of Tyr 62, Asp 197, Glu 233, Asp 300, His 305, and Ala 307 active residues of α-amylase. β-Caryophyllene epoxide was found to be the most active inhibitor with a docking score of -8.3050 and formed five hydrogen bonds at the active site residues of α-amylase. Asp 197, Glu 233, and Asp 300 active residues were observed to be making polar interactions with the ligand. Conclusions. The current study revealed that Ph.Lo is rich in bioactive metabolites which might contribute to its enzyme inhibitory potential. Inhibition of these enzymes is the key target in reducing postprandial hyperglycemia. However, further detailed in vivo studies are required for their biological and therapeutic activities.

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Zahara, Meutia, Mauizah Hasanah, and Rahma Zalianda. "Identification of Zingiberaceae as medicinal plants in Gunung Cut Village, Aceh Barat Daya, Indonesia." Journal of Tropical Horticulture 1, no. 1 (November 9, 2018): 24. http://dx.doi.org/10.33089/jthort.v1i1.9.

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Zingiberaceae is well known as gingers family with abundant numbers of spices widely used in Southeast Asia, because of their unique flavors, ornamental and also the medicinal values. The extracts of Zingiberaceae rhizome contain many essentials oils, including terpenes, alcohols, ketones, flavonoids and phytoestrogens that use as medicine. Zingiberaceae grow vigorously in a wide range of habitats ranging from riverine to the limestone area, from lowland to the upper mountain regions. They often grow in shady area but some of the native species are able to tolerate to the sun exposure. The cultivation of this family was developed widely. Gunung Cut Village is one the villages in Aceh Barat Daya district which cultivated four species of this family: Zingiber officinale, Curcuma domestica, Alpina galanga and Kaempferia galanga. They mainly use for medicinal purposes as it can cure many diseases traditionally.

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Journal articles on the topic 'Essentials oils'

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