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Relevant bibliographies by topics / Escherichia Identification

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Academic literature on the topic 'Escherichia Identification'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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Journal articles on the topic "Escherichia Identification"

1

Parin, U., S. Kirkan, SS Arslan, and HT Yuksel. "Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea." Veterinární Medicína 63, No. 3 (March 28, 2018): 110–16. http://dx.doi.org/10.17221/156/2017-vetmed.

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The aim of this study was to determine the incidence of Escherichia fergusonii in dairy cattle with clinical signs of diarrhoea. The specimens were obtained from three different farms in Denizli province of Turkey, between August 2016 and December 2016. Rectal contents of 57 Holstein-friesian dairy cattle with diarrhoea were collected from farms located in the Aegean Region (Denizli province, Turkey). Rectal swabs were inoculated into enrichment, differential and selective culture media. A total of 49 (86%) Escherichia spp. were isolated by phenotypic identification from 57 rectal swab samples. Presumptive E. fergusonii isolates were tested with the API 20E identification kit and all isolates (100%) were identified as E. coli. Primers targeting specific E. coli and E. fergusonii and genes, including the beta-glucuronidase enzyme, conserved hypothetical cellulose synthase protein and regulator of cellulose synthase and hypothetical protein, putative transcriptional activator for multiple antibiotic resistance were used for detection and differentiation of E. coli and E. fergusonii. Thirteen of the 49 E. coli-verified isolates were identified as E. fergusonii after duplex PCR using EFER 13- and EFER YP-specific primers. Confirmation of strain identity was conducted using Sanger sequencing analysis. The rates of antibiotic resistance of E. fergusonii to penicillin G and erythromycin were 100% and 77%, respectively. In conclusion, field strains of E. fergusonii were detected in cattle with diarrhoea in Turkey, and the strains were found to be resistant to multiple antibiotics.

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Hachim Mohammed, Husham, Mohammed Flayyih Tareef, and Ali Kamal Mohammed. "Molecular Identification of Isolate from Escherichia coli Isolates from Dialysis Patients." Journal of Pure and Applied Microbiology 12, no. 4 (December 30, 2018): 2087–94. http://dx.doi.org/10.22207/jpam.12.4.45.

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Freestone, P., S. Grant, I. Toth, and V. Norris. "Identification of phosphoproteins in Escherichia coli." Molecular Microbiology 15, no. 3 (February 1995): 573–80. http://dx.doi.org/10.1111/j.1365-2958.1995.tb02270.x.

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Lindsey, Rebecca L., L. Garcia-Toledo, D. Fasulo, L. M. Gladney, and N. Strockbine. "Multiplex polymerase chain reaction for identification of Escherichia coli , Escherichia albertii and Escherichia fergusonii." Journal of Microbiological Methods 140 (September 2017): 1–4. http://dx.doi.org/10.1016/j.mimet.2017.06.005.

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York, Mary K., Ellen Jo Baron, Jill E. Clarridge, Richard B. Thomson, and Melvin P. Weinstein. "Multilaboratory Validation of Rapid Spot Tests for Identification of Escherichia coli." Journal of Clinical Microbiology 38, no. 9 (2000): 3394–98. http://dx.doi.org/10.1128/jcm.38.9.3394-3398.2000.

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To validate the accuracy of rapid tests for identification ofEscherichia coli, five laboratories sequentially collected 1,064 fresh, clinically significant strains with core criteria of indole-positive, oxidase-negative, nonspreading organisms on sheep blood agar plates (BAP), having typical gram-negative rod plate morphology, defined as good growth on gram-negative rod-selective media. An algorithm using beta-hemolysis on BAP, lactose reaction on eosin-methylene blue or MacConkey agar,l-pyrrolidonyl-β-naphthylamide (PYR), and 4-methylumbelliferyl-β-d-glucuronide (MUG) was evaluated. Identifications using the algorithm were compared to those obtained using commercial kit system identifications. One thousand strains wereE. coli and 64 were not E. coli by kit identifications, which were supplemented with conventional biochemical testing of low probability profiles. Of the 1,064 isolates meeting the core criteria, 294 were beta-hemolytic and did not require further testing to be identified as E. coli. None of the 64 non-E. coli strains were hemolytic, although other indole-positive, lactose-negative species were found to be hemolytic when further strains were examined in a follow-up study. Of the remaining strains, 628 were identified as E. coli by a lactose-positive and PYR-negative reaction. For nonhemolytic, lactose-negative E. coli, PYR was not helpful, but a positive MUG reaction identified 65 of 78 isolates as E. coli. The remaining 13 E. coli strains required kit identifications. This scheme for E. coli identification misidentified three non-E. coli strains as E. coli, for an error rate of 0.3%. A total of 13 kit identifications, 657 PYR tests, and 113 MUG tests were needed to identify 1,000 E. coli strains with the algorithm. The use of this rapid system saves laboratory resources, provides timely identifications, and yields rare misidentifications.

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Lee, Yun-Song, and Myung-Hee Chung. "Identification of Escherichia coli 8-oxoguanine endonuclease." Experimental & Molecular Medicine 32, no. 3 (September 2000): 155–60. http://dx.doi.org/10.1038/emm.2000.26.

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Ero, R., L. Peil, A. Liiv, and J. Remme. "Identification of pseudouridine methyltransferase in Escherichia coli." RNA 14, no. 10 (August 28, 2008): 2223–33. http://dx.doi.org/10.1261/rna.1186608.

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Rotar, Ancuta Mihaela, Cristina Anamaria Semeniuc, Sorin Apostu, Carmen Pop, Mihaela Duma, Ramona Suharoschi, and Larisa Giura. "Identification and Prevalence of Escherichia coli and Escherichia coli O157: H7 in Foods." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology 70, no. 2 (November 13, 2013): 139. http://dx.doi.org/10.15835/buasvmcn-fst:9392.

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The objective of this study is to investigate the incidence of Escherichia coli in animal and non-animal foods, and mainly the incidence of the serotype O157: H7 producing verotoxin. The presence of common Escherichia coli and Escherichia coli O157: H7 in various foods (of animal and non animal origin) was performed in Transylvania area. We analyzed a total of one hundred forty-one samples of minced meat, one hundred twenty-six samples of meat , twenty six samples of meat products, five samples of alcoholic beverages, three samples of seafood, one hundred samples of cheese from pasteurized milk, seventeen samples of butter, four samples of vegetables and one sample of milk powder, using the standard cultural method and Vidas Eco method for E. coli O157: H7 strains. E. coli was identified in 50 samples of minced meat, 55 samples of meat prepared, 4 samples of meat products, 2 samples of alcoholic beverages, 25 samples of cheese from pasteurized milk, 6 samples of butter and 1 sample of vegetables. In this study were not been identified any foods contaminated with the E. coli O157: H7 serotype. The results of this reasearch have demostrated that E. coli wich represents a hygienic indicator of recent food contamination, can be destroyed with heat treatment and hygienic handling of foods. Our country over the years has been among the few countries where the incidence of the E. coli O157: H7 serotype has been minimal.

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Blum, S., E. D. Heller, O. Krifucks, S. Sela, O. Hammer-Muntz, and G. Leitner. "Identification of a bovine mastitis Escherichia coli subset." Veterinary Microbiology 132, no. 1-2 (November 2008): 135–48. http://dx.doi.org/10.1016/j.vetmic.2008.05.012.

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N, Padmaja, Anand Acharya, and Nageswar Rao P. "IDENTIFICATION OF UROVIRULENT MARKERS IN UROPATHOGENIC ESCHERICHIA COLI." Journal of Evolution of Medical and Dental Sciences 1, no. 4 (October 30, 2012): 578–81. http://dx.doi.org/10.14260/jemds/90.

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Dissertations / Theses on the topic "Escherichia Identification"

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Bourbonneux, Valéry. "Identification des "Escherichia" par auxanogramme." Paris 5, 1993. http://www.theses.fr/1993PA05P139.

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Kerner, Michael Johannes. "The Escherichia coli GroEL interaction proteome identification and classification /." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=979094593.

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Kiel, Michael Christopher. "Identification and characterization of an Escherichia coli ribosomal ATPase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0024/NQ50055.pdf.

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Jarboe, Laura Renee. "Identification and simulation of regulatory networks in Escherichia coli." Diss., Restricted to subscribing institutions, 2006. http://proquest.umi.com/pqdweb?did=1276395911&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Blanchard, Geneviève. "Versatilité nutritionnelle de l'espèce génomique "Escherichia Coli-Shigella"." Paris 5, 1990. http://www.theses.fr/1990PA05P055.

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DIEKERT, PASCALE. "Methodes d'identification et de denombrement des coliformes fecaux et d'escherichia coli." Strasbourg 1, 1990. http://www.theses.fr/1990STR15043.

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Christensen-Dalsgaard, Mikkel. "Identification and characterization of new messenger RNA interferases of Escherichia coli." Thesis, University of Newcastle Upon Tyne, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555996.

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Toxin-antitoxin (TA) loci, which are widely distributed in prokaryotes, are organized in small operons, consisting of genes encoding toxins and antitoxins. Transcription from the promoter is regulated by the TA protein complex. Activation of the toxin occurs when the toxin is in excess of the antitoxin, for example during nutritional stress when the labile antitoxins are rapidly degraded by cellular proteases. The biological function of TA systems is debated and several different models have been proposed. Several of the TA loci encode messenger RNA interferases (mls) that inhibit translation by cleaving mRNA. Two types are known: those that cleave mRNA codons at the ribosomal A-site and those that cleave any RNA site-specifically. In this work, it was shown that the ml, YoeB cleaved mRNA strictly dependent on translation in vivo. Furthermore, it was shown that site-specific mRNA cleavage by MazF occurs independently of translation but that translation can seriously influence MazF cleavage efficiency. Expression of the toxin Doc of phage P1 was shown to mediate mRNA cleavage, though activation of the endogenous E. coli RelE ml. In a different study, three new ml encoding TA loci of E. coli were identified and characterized. Ectopic expression of the three ml genes lead to a rapid growth arrest caused by inhibition the global rate-of-translation. Furthermore, ml induced degradation of several different model RNAs were observed in vivo. Two of the mls cleaved only translated RNAs, similar to RelE and YoeB, whereas one ml cleaved both in coding and non-coding regions of the RNAs, resembling the properties of MazF and ChpBK toxins. Transcription of the three TA mRNAs was induced by various stressful conditions and dependent on the proteases Lon and Clp. In a different section of this work, a new synthetic lethal screen was developed using antisense RNA regulated R1 plasmids. The screen was used to search for synthetic lethal of sick interactions with the molecule responsible for trans-translation, tmRNA. Two genes were found to be synthetic sick with tmRNA: the tatC gene, which encodes an essential component of the twin- ariginine translocation complex and the dksA gene, which encode a transcription factor responsible for regulation of ribosomal RNA promoters.

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Riley, Laura Maryse. "Identification of Bacteriophage 024B- Encoded Genes Expressed by Escherichia coli Lysogens." Thesis, University of Liverpool, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510970.

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Sarica, Nazim. "Identification de contraintes locales impactant l’expression des gènes chez Escherichia coli." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASL021.

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L'expression des génomes dépend de toutes les « contraintes » qui s'appliquent à l'ADN, que ce soit au niveau nucléotidique et génique (1D), qu'au niveau de son organisation conformationnelle (3D). Les études menées chez les bactéries ont montré que l'organisation 3D de leur chromosome circulaire s'échelonne du niveau moléculaire au niveau cellulaire. Au niveau moléculaire, les NAPs (Nucleoid Associated Proteins) organisent l'ADN en microdomaines d'environ 10 kb qui présentent des surenroulements de l'hélice d'ADN indépendants (Postow et al., 2004). Au niveau cellulaire, le chromosome est organisé en 4 régions structurées et 2 autres non structurées, appelées macrodomaines, d'une taille proche de 1Mb (Valens et al., 2004). Le projet consiste à étudier l'impact de contraintes topologiques sur la transcription des gènes en fonction de la position chez E. coli. La taille élevée du chromosome et les éléments interagissant avec rendent variable et dynamique l'organisation chromosomique. Il est donc difficile d'étudier certains aspects de la structure du chromosome et de corréler ces résultats avec l'expression génique
A long list of constrains that affect gene expression have been discovered thanks to the thousands of sequenced genomes and comparative genomics. These contrains can be at the nucleotidic level, but also at the 3D scale. Studies showed that the spatial organization of bacterial genomes goes from the molecular level to the cellular level. At the molecular level, NAPs (Nuceloid Associated Proteins) are modeling the chromosome by inducing 10kb loops called microdomains, that present different supercoiling levels. At the cellular scale, it has been observed 4 different structured regions + 2 non-structured regions on E.coli's chromosome. These regions of approximatively 1Mbp are called macrodomains. The first goal of this project is to study the effects of positioning and constrains on gene expression in E.coli. With the chromosome being so large and precisely organized in time and space by several interacting elements simultaneously, it is often difficult for researchers to isolate one specific feature and to accurately correlate this to gene regulation. What becomes obvious when one begins to wade through the literature is that the field would greatly benefit from simplified model chromosomes

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Baertlein, Dawn Marie August. "Identification of phosphate starvation inducible mineral phosphate solubilization genes in Escherichia coli." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184606.

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Under conditions of phosphate starvation Escherichia coli can solubilize mineral phosphates, such as dicalcium phosphate, to orthophosphate which is then available for uptake and cell growth processes. lac operon fusions were created using MudX phage, and mineral phosphate solubilization (Mps) mutants were identified by their inability to solubilize mineral phosphate on plate assays. Four of these mutants have been mapped on the E. coli chromosome via Hfr matings and are located at two distinct portions of the chromosome; between 23 and 50 minutes and between 60 and 90 minutes. One mutant in each region has phosphate starvation inducible (Psi) promoter activity. One of these mutants (DB1047) was mapped to between 69 and 75 minutes via F' matings, and fine structure mapped to 75 minutes by hybridization with λ clones from a genomic library of Escherichia coli. DB1047 was characterized more closely and found to exhibit pleiotropy with regard to several membrane related traits. Evidence that this is a single insertional event comes from the simultaneous loss of all traits tested in spontaneous revertants. Additionally, a Tn5 mutant was identified that was identical for these traits. Our data strongly support the hypothesis that the mutation carried by DB1047 is in the ompB locus. This locus consists of the two regulatory cotranscribed genes, ompR and envZ. This locus is involved in regulation of transcription of the ompC and ompF genes for outer membrane porin proteins, and is located at 75 minutes on the chromosome as is the DB1047 mutation. DB1047 lacks the outer membrane porin OmpF, a phenotype previously attributed to envZ mutants. However, the ompR321 mutant resembles DB1047 in reduced ability to solubilize phosphate. Additional supporting evidence for the DB1047 mutation belonging to the ompB locus comes from the most recent report that mutations in the himA gene, which I found to be deficient in the ability to solubilize phosphate, also affect the regulation of production of the outer membrane porin OmpF.

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Books on the topic "Escherichia Identification"

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Cinotto, Peter J. Occurrence of fecal-indicator bacteria and protocols for identification of fecal-contamination sources in selected reaches of the West Branch Brandywine Creek, Chester County, Pennsylvania. Reston, Va: U.S. Geological Survey, 2005.

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United States. Environmental Protection Agency. Office of Research and Development. Microbial source tracking guide document. Cincinnati, Oh: U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 2005.

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Patel, Samir D. Identification of a mutation in the Asparaginyl-tRNA synthetase gene of N4316, a mutant of Escherichia coli that is deficient in translation. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1993.

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Freundlieb, Sabine. Periplasmic amylase of Escherichia coli: Identification of its structural gene, malS, isolation of the protein, and its application for in vivo analysis of the lamB channel. Konstanz: Hartung-Gorre, 1988.

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5

Kiel, Michael Christopher. Identification and characterization of an escherichia coli ribosomal ATPase. 2000.

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Lee, Jong Ho. Identification and characterization of a new gene essential for production of formate hydrogenlyase activity in Escherichia coli. 1987.

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Choi, Mieyoung. Sequencing and cloning of the N4-coded single-stranded DNA binding protein gene: Identification and functional analysis of active domains. 1992.

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Book chapters on the topic "Escherichia Identification"

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Nouwens, Amanda S., Femia G. Hopwood, Mathew Traini, Keith L. Williams, and Bradley J. Walsh. "Proteome Approach to the Identification of Cellular Escherichia coli Proteins." In Organization of the Prokaryotic Genome, 331–46. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818180.ch18.

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Gannon, V. P. J., S. D’Souza, T. Graham, and R. K. King. "Specific Identification of Escherichia coli O157:H7 Using a Multiplex PCR Assay." In Advances in Experimental Medicine and Biology, 81–82. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1828-4_10.

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Bletz, Stefan, Alexander Mellmann, and Barbara Middendorf-Bauchart. "Identification of Shiga Toxin-Producing Escherichia coli Outbreaks Using Whole Genome Sequencing." In Methods in Molecular Biology, 87–97. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1339-9_3.

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Kashiwagi, Keiko, and Kazuei Igarashi. "Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae." In Methods in Molecular Biology, 295–308. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-034-8_18.

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Mori, Hirotada, Tomoya Baba, Katsushi Yokoyama, Rikiya Takeuchi, Wataru Nomura, Kazuichi Makishi, Yuta Otsuka, Hitomi Dose, and Barry L. Wanner. "Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12." In Gene Essentiality, 45–65. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2398-4_4.

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Ruano-Gallego, David, and Luis Ángel Fernández. "Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing Escherichia coli to Epithelial Cells." In Methods in Molecular Biology, 253–72. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1339-9_11.

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Chu, Shijian, and Trevor J. Trust. "Identification and Characterization of an Aeromonas salmonicida Gene Which Affects A-Protein Expression in Escherichia coli." In Advances in Bacterial Paracrystalline Surface Layers, 311–13. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9032-0_34.

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Almendros, Cristóbal, and Francisco J. M. Mojica. "Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification of Target Interference Motifs in Escherichia coli." In Methods in Molecular Biology, 161–70. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2687-9_10.

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Leimkühler, Silke, and K. V. Rajagopalan. "Identification of the Sulfurtransfer Pathway for the Generation of the Dithiolene Moiety of Molybdopterin in Escherichia Coli." In Chemistry and Biology of Pteridines and Folates, 253–57. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0945-5_42.

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Babu, Mohan, Gareth Butl, Oxana Pogoutse, Joyce Li, Jack F. Greenblatt, and Andrew Emili. "Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli." In Proteomics, 373–400. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-157-8_22.

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Conference papers on the topic "Escherichia Identification"

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Yao, Mingyin, Jinlong Lin, Muhua Liu, Qiulian Li, Zejian Lei, and Lin Huang. "Identification of escherichia coli by laser induced breakdown spectroscopy." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639492.

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Feng, Jian, Yan-hong Bai, Yun-long Wang, and Jian-zhou Jing. "Detection and Identification of Enterohemorrhagic Escherichia coli O157:H7 Using Agilent 2100 Bioanalyzer." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5515776.

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Abrar, Mahdi, Teuku Reza Ferasyi, Amiruddin, Fakhrurrazi, Erina, Rusli Sulaiman, Rina Aulia Barus, Teuku Shaddiq Rosa, and Rezky Ramadhan. "Isolation and Identification of Escherichia coli Serotype O157 from Swabs of Rectal Faeces in Aceh Cattle." In 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/absr.k.210420.039.

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Yongmei Li and Fan Li. "Identification and characterization of class 1 integron-mediated antibiotics resistance among Shiga toxin-producing Escherichia coli isolates." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5966141.

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Yan, Yaxian, Huiying Zhang, Luming Xia, Liangke Shu, and Jianhe Sun. "Identification and Characterization of stx2 Converting Bacteriophage from Shiga-Toxin-Producing Escherichia coli O157 Strains of Animal Origin." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162916.

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Promponas, Vasilis J. "A simple clustering approach for pathogenic strain identification based on local and global amino acid compositional signatures from enomic sequences: the Escherichia genus case." In 2009 9th International Conference on Information Technology and Applications in Biomedicine (ITAB 2009). IEEE, 2009. http://dx.doi.org/10.1109/itab.2009.5394396.

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Cho, Myoung-Ock, Hyo Mi Chang, Yeon Gyu Yu, Hwataik Han, and Jung Kyung Kim. "Selective and Automated Detection of Airborne Asbestos Fibers Using Chrysotile-Adhesive Protein and High-Throughput Microscopy (HTM)." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-63721.

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There are several methods to detect asbestos including phase contrast microscopy (PCM), polarized light microscopy, X-ray diffraction, and electron microscopy. Although the PCM method is widely used due to its simple process and relatively low cost, it is a time-consuming and laborious process that is manually performed by a human counter. We developed a high-throughput microscopy (HTM) system for automated counting of airborne asbestos fibers to automate the conventional PCM method. Our results show that automatic image acquisition by synchronization of charge-coupled device (CCD) camera with movement of stages, and image analysis using image processing software, significantly reduced time consumption and labor. In this study, we used DksA chrysotile-adhesive protein for the selective detection of asbestos. DksA, known as the protein that specifically attaches to chrysotile, was extracted from Escherichia coli through a recombinant protein technique. We tried to detect chrysotile selectively from other fibers or particles, and we developed a highly selective and automated low-cost device for automated identification and enumeration of airborne asbestos fibers based on the HTM method.

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Berechet, Mariana Daniela, Demetra Simion, Maria Stanca, Cosmin Andrei Alexe, Rodica Roxana Constantinescu, Maria Rapa, and Andreea Turcanu. "Antibacterial and antioxidant activities of lemon balm (Melissa officinalis L.) essential oil." In The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.2.

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Lemon balm (Melissa officinalis L.) belongs to the Lamiaceae family. Essential oil extracted from the aerial parts of lemon balm has been investigated for the protection of fruits during storage, as insecticidal, as well as in medicine, due to its bioactive properties. In this paper, the composition and identification of components from Melissa officinalis L. essential oil were determined by gas chromatography coupled with mass spectrophotometry (GC/MS) analysis. Total phenol content (TPC) and the scavenging activity towards 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and 2,2′-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS+·) free radicals were evaluated by UV-VIS spectrometry. Antibacterial activities were carried out against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Seventeen bioactive compounds were found as constituents of Melissa officinalis L. essential oil, among which o-cymene (19.735%), dehydro-p-cymene (17.180%), and limonene (11.589%) were found as the major components. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) provided a confirmation for the chemical components of lemon balm essential oil identified by GC/MS. The values recorded for TPC and antioxidant activity were as follows: 54.72 mg GAE/g dry substance, 28.53% for DPPH, and 46.17% for ABTS assays, respectively. 100 µL lemon balm essential oil proved total antibacterial activity against the tested microorganisms. The results showed that the Melissa officinalis L. may be a good candidate as plant-derived antioxidant and antibacterial agent for medical footwear, wound dressings and other medical applications.

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Mack, Andrew, and Julie A. Funk. "Abbreviated identification scheme for Eschericia coli in swine feces." In Second International Symposium on Epidemiology and Control of Salmonella in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-469.

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