Journal articles on the topic 'EPSILON NEGATIVE MEDIA (ENG)'

We propose a design of narrow-band multi-channel optical filter using multiple stacks of unit cell consisting of trilayer of Doubly positive medium (DPS)–Doubly negative medium (DNG)–Epsilon negative medium (ENG). Finite Difference Time Domain (FDTD) technique is used to numerically simulate the effect of optical thickness of DPS, DNG and ENG media on tunability of the transmission spectrum of such a medium. We also demonstrate the control on the number of channels at different frequencies that can be transmitted through such medium.

This article discusses the physical theory and mathematical modeling of epsilon-negative (ENG) material. Numerical simulation results are also given in later part of this article, and they prove that this kind of material has a great shielding effect. We mainly focus on the modeling and simulation of one dimensional epsilon-negative (ENG) material. First we set up a Drude model to theoretically simulate ENG material, and then use the finite difference time-domain methods to analyze the electromagnetic wave propagation in our shielding material.

A comprehensive review of the fundamentals and applications of epsilon-negative materials is presented in this paper. Percolative composites, as well as homogeneous ceramics or polymers, have been investigated to obtain the tailorable epsilon-negative properties. It's confirmed the anomalous epsilon-negative property can be realized in conventional materials. Meanwhile, from the perspective of materials science, the relationship between the negative permittivity and the composition and microstructure of materials has been clarified. It's demonstrated that the epsilon-negative performance is attributed to the plasmonic response of delocalized electrons within the materials and can be modulated by it. Moreover, the potential applications of epsilon-negative materials in electromagnetic interference shielding, laminated composites for multilayered capacitance, coil-less electric inductors, and epsilon-near-zero metamaterials are reviewed. The development of epsilon-negative materials has enriched the connotation of metamaterials and advanced functional materials, and has accelerated the integration of metamaterials and natural materials.

This paper presents a new planar particle that shows negative effective permittivity under irradiation by an electromagnetic wave. The mutual coupling between the couples of these particles is studied in particular. The response of this particle sensitive to an electric field is strongly anisotropic. The particle is aimed to be used to compose an isotropic epsilon-negative metamaterial in two forms. First, a unit cell of the metamaterial consists of a cube bearing six particles on its faces, located with specific orientations. The experiments showed that this unit cell is suitable for manufacturing an isotropic epsilon-negative metamaterial obtained by arranging these cells in a 3D cubic periodic system. The second form of an epsilon-negative metamaterial with an isotropic response consists of the planar particles themselves, distributed quasi-randomly, composing a 2D system and/or of particles placed in spherical shells and distributed fully randomly in a hosting material forming a 3D system. The isotropy of these systems was verified by measurements in a rectangular waveguide.

A high gain omnidirectional antenna with low profile is proposed and is investigated numerically and experimentally. Based on the conventional center-fed circular epsilon-negative (ENG) zeroth-order resonator (ZOR) antenna, dendritic structure negative permeability metamaterial (NPM) is used as the substrate to enhance the gain of the omnidirectional antenna. The experimental results show that the gain of a center-fed circular ENG ZOR antenna with NPM substrate is enhanced about 2.2 dB, and the efficiency is enhanced about 38%, in the whole broad operating bandwidth as compared to that of the antenna without NPM substrate, which can be used to improve the reliability of wireless communications.

In this work, we developed a unique mathematical model to solve dispersion relation for surface polaritons (SPs) in artificial composite materials grating. Here, we have taken two types of materials for analysis. In the first case, the grating composed of epsilon-negative (ENG) material and air interface. In second case, grating composed of left-handed materials (LHMs) and ENG medium interface is considered. The dispersion curves of both p and s polarized SPs modes are obtained analytically. In the case of ENG grating and air interface, polaritons dispersion curves exist for p-polarization only, whereas for LHM grating and ENG medium interface, the polaritons dispersion curves for both p and s polarization are observed.

Radio frequency (RF) blackout and attenuation have been observed during atmospheric reentry since the advent of space exploration. The effects range from severe attenuation to complete loss of communications and can last from 90 s to 10 min depending on the vehicle’s trajectory. This paper examines a way of using a metasurface to improve the performance of communications during reentry. The technique is viable at low plasma densities and matches a split-ring resonator (SRR)-based mu-negative (MNG) sheet to the epsilon-negative (ENG) plasma region. Considering the MNG metasurface as a window to the exterior of a reentry vehicle, its matched design yields high transmission of an electromagnetic plane wave through the resulting MNG-ENG metastructure into the region beyond it. A varactor-based SRR design facilitates tuning the MNG layer to ENG layers with different plasma densities. Both simple and Huygens dipole antennas beneath a matched metastructure are then employed to demonstrate the consequent realization of significant signal transmission through it into free space beyond the exterior ENG plasma layer.

Decreasing the Radar Cross Section (RCS) is investigated in electromagnetic materials, i.e. double-positive (DPS) , double-negative (DNG) , epsilon-negative (ENG) and mu-negative (MNG) materials. The interesting properties of these materials lead to a great flexibility in manufacturing structures with unusual electromagnetic characteristics. The valid conditions for achieving the transparency and gaining resonance for an electrically small cylinder are established, in this corresponding The effect of incidence direction on RCS inclusive of transparency and resonance conditions is also explored ,through computer simulations for an electrically small cylinder.

This study presents a double-inverse-epsilon-shaped, triple-band epsilon-negative (ENG) metamaterial with two split ring resonators (SRRs). The proposed unit cell comprises a single slit two SRRs with two inverse-epsilon-shaped metal bits. Rogers RT6002, of dimension 10 × 10 × 1.524 mm3, is used as a substrate. An electromagnetic simulator CST microwave studio is used to investigate the effective medium parameters of the material. The proposed metamaterial shows three resonance peaks that are demarcated at the frequencies 2.38 GHz, 4.55 GHz and 9.42 GHz consecutively. The negative permittivity of the metamaterial is observed at the frequency ranges of 2.39–2.62 GHz, 4.55–4.80 GHz and 9.42–10.25 GHz. The goodness of the material was presented by the effective medium ratio (EMR) of the unit cell at 12.61. In addition, the simulated results are authenticated by using different electromagnetic simulators such as HFSS and ADS for the equivalent circuit model, which exhibits insignificant disparity. The anticipated scheme was finalised through some parametric analyses, together with configuration optimisation, different unit cell dimensions, several substrate materials, and altered electromagnetic (EM) field transmissions. The proposed triple band (S-, C- and X-bands) with negative permittivity (ε) metamaterial is practically used for numerous wireless uses, for instance, far distance radar communication, satellite communication bands and microwave communication.

The transmission properties of a periodic structure consisting of alternating metal layers and air layers are studied by numeric methods. The metal layers are the epsilon-negative (ENG) materials. The interaction of the evanescent waves in metal layers and propagation waves in air layers forms some special transmission bands. Given proper structure parameters, one transmission frequency only allows one incident direction in the low frequency range. This structure can simultaneously achieve frequency filtering and direction filtering.

AbstractIn the first part, the scattering characteristics of an isolated dielectric coated conducting rod have been investigated. The types of considered coatings for the scattering analysis are realistic materials including barium strontium titanate, magnetodielectric, gallium arsenide, and silicon carbide. It is found that the gallium arsenide coating can be used to significantly reduce the scattering from a thin perfectly electric conducting cylindrical rod at specific observation angles. In the second part, the effective permittivity and permeability of metamaterials composed of two dimensional periodic arrangements of these dielectric coated conducting cylindrical rods have been studied. An increase in the double negative (DNG) bandwidth of a metamaterial composed of barium strontium titanate coated conducting rods has been observed in contrast to the corresponding bandwidth of a metamaterial composed of only barium strontium titanate material rods. Also an additional plasmonic epsilon negative (ENG) bandwidth has been found in case of a metamaterial composed of barium strontium titanate coated conducting rods. It is further studied that the widest ENG, mu negative, and DNG bandwidths exist for a metamaterial composed of gallium arsenide rods.

This paper proposes the design of a four-element array planar antenna based on a single antenna that combines the Double Positive (DPS) and Epsilon Negative (ENG) materials. The single antenna consists of a microstrip segment (which is equivalent to a DPS material) connected to a grounded microstrip segment (which is equivalent to an ENG material). T-Junction power dividers with one-input and two-output ports are used for feeding the two-element and the four-element array antennas. The proposed array antenna is designed to operate optimally at 30GHz frequency under Finite Element Method (FEM)-based simulation. The obtained simulation results show that the proposed array antennas have good radiation performances, in which the four-element array antenna has a -10dB bandwidth ranging from 28.7 to 33.4GHz and 12.9dBi gain.

Near-perfect resonant absorption is attained in a single-polarization single-mode photonic crystal fiber (SPSM PCF) within the long-wave infrared (LWIR) range from 10 to 11 μm. The basic PCF design is a triangular lattice-based cladding of circular air holes and a core region augmented with rectangular slots. A particular set of air holes surrounding the core is partially filled with SiC, which exhibits epsilon near-zero (ENZ) and epsilon negative (ENG) properties within the wavelength range of interest. By tuning the configuration to have the fields of the unwanted fundamental and all higher order modes significantly overlap with the very lossy ENG rings, while the wanted fundamental propagating mode is concentrated in the core, the SPSM outcome is realized. Moreover, a strong plasmonic resonance is attained by adjusting the radii of the resulting cylindrical core-shell structures. The cause of the resonance is carefully investigated and confirmed. The resonance wavelength is shown to finely shift, depending on the relative permittivity of any material introduced into the PCF’s air holes, e.g., by flowing a liquid or gas in them. The potential of this plasmonic-based PCF structure as a very sensitive, short length LWIR spectrometer is demonstrated with an environmental monitoring application.

In this paper, we propose a reconfigurable metasurface antenna for beam switching applications. The reconfigurable metasurface is formed by uniformly distributed double-split square rings loaded with positive-intrinsic-negative (PIN) diodes for dual operations of a wave reflector and a wave director. Specifically, when the PIN diodes are forward biased, an epsilon-negative (ENG) metasurface is realized which reflects all incident waves with appropriate polarization; when the diodes are reverse biased, at the same operating frequency, a mu-near-zero (MNZ) metasurface is acquired which directs wave propagation. For excitation, a dipole radiator loaded with the same type of PIN diode is designed. Simulation and measurement results show good agreement and verify the beam switching functionality of the proposed metasurface antenna.

Abstract A robust and simple mid-infrared hollow-core anti-resonant fiber (ARF) based refractive index (RI) sensor with an intensity demodulation method is presented and analyzed for monitoring liquid analytes. The ARF allows liquid analytes to flow through its hollow area for detection. To obtain ideal sensing performance, an epsilon negative (ENG) material is introduced into the selected anti-resonant tube. With the high absorption of the ENG material, only one fundamental mode is available for detection and is sensitive to the RI variation of analytes. Moreover, the effects of structural parameters on the sensing performances are discussed and analyzed to further understand the mechanism and optimization. The final result shows that the ARF sensor can exhibit a high sensitivity of −372.58 dB RIU−1 at a fixed wavelength within a broad RI range from 1.33 to 1.45, which covers most liquid analytes. It is a promising candidate for chemical and environmental analysis. Additionally, it has the potential for deep research to feed diverse applications.

In this paper, we introduce and review the zeroth-order resonator (ZOR) antennas with outstanding characteristics including various applications that have been researched so far. Since the zeroth-order resonance frequency is independent of a physical length of antenna, the ZOR antenna can theoretically be designed quite small and have a possibility to apply to considerably lots of applications. First, we have presented the ZOR antennas implemented by double-negative (DNG), epsilon-negative (ENG), and mu-negative (MNG) transmission lines. Then, the research related on extremely small, wide beamwidth, wideband, and circularly polarized (CP) ZOR antennas have been continuously carried out. Based on a series of these studies, the ZOR antennas were utilized for various applications such as a wireless power transfer (WPT), a compact controlled reception pattern antenna (CRPA), a penta-band mobile antenna, and a wide steering array antenna.

A triple-band epsilon-negative (ENG) metamaterial based on a split ring resonator (SSR) with a modified hexagonal-shaped metal strip proposed in this study is a new combination of a single slit square resonator and a modified hexagonal-shaped metal strip. The desired unit cell FR-4 (lossy) that was selected as the substrate was 1.6 mm thick. Following the assessment of the unit cell, a high-frequency electromagnetic simulator like the computer simulation technology (CST) microwave studio was applied to assess the S-parameters. The proposed design exhibited resonance at 2.89, 9.42, and 15.16 GHz. The unit cell also demonstrated negative permittivity in the frequency ranges 2.912–3.728 GHz, 9.552–10.144 GHz, and 15.216–17.328 GHz, along with a negative refractive index. An effective medium ratio (EMR) of 11.53 is an indicator of the goodness of the metamaterial unit cell. It is deliberate at the lowermost resonance frequency of 2.89 GHz. Moreover, the simulated results that were validated using HFSS and equivalent circuit model indicated slight variations. The proposed design was finalised based on several parametric studies, including design optimisation, different unit cell sizes, various substrate materials, and different electromagnetic (EM) field propagations. The proposed triple band (S, X, and Ku bands) negative permittivity metamaterial unit cell can be utilised for various wireless applications, such as microwave communication, satellite communication, and long-distance radio communication.

This paper proposes one-dimensional antenna arrays of the four-element and the eight-element using composite materials. Firstly, the single element is designed to resonate at Zeroth-order using a pair of Double positive (DPS) and Epsilon negative (ENG) materials meta-structured transmission line (MTL). Secondly, three of 1:2 T-Junction power dividers and seven of 1:2 T-Junction power dividers based on micro-strip technology are designed for feeding the four-element and the eight-element array antennas, respectively. Finally, the proposed arrays are optimized using FEM-based simulation to operate at the frequency of 8,5 GHz. The simulated results show that both antenna arrays have Zeroth-order resonance (ZOR) property, in which the four-element array has a bandwidth spreading from 8.39 to 8.61 GHz and a maximum gain of 8.82 dB while the other one of the eight-element array is 8.39 – 8.60 GHz and 12.2 dB, respectively. The proposed array antennas can be used for wireless applications or mobile communications.

Abstract In this paper, a new single negative metamaterial (MTM) unit cell structure with low profile of 6.2 × 6.2 m2 is proposed. The unit cell shows near zero refractive index (NZRI) characteristics in C, X and Ku bands whereas epsilon negative index (ENG) in Ku-Band frequency. The properties of the proposed MTM unit cell are analysed with the Rogers 5880 substrate which displays low permittivity. To verify the effectiveness of the split ring resonators (SRR) as a metamaterial, a comparison of the unit cell properties in 2 × 3 array structures is created. Besides low profile, tunability performance is shown in the proposed design by adjust the central strip line width of the proposed metamaterial whereas the frequency resonance can be shifted and controlled accordingly. Using commercially available CST software, the reflection (S11) and transmission (S12) parameters of the unit cell are determined. The proposed MTM unit cell can be potentially used in many applications including satellite communication systems.

In this paper, a compact textile ultrawideband (UWB) planar monopole antenna loaded with a metamaterial unit cell array (MTMUCA) structure with epsilon-negative (ENG) and near-zero refractive index (NZRI) properties is proposed. The proposed MTMUCA was constructed based on a combination of a rectangular- and a nonagonal-shaped unit cell. The size of the antenna was 0.825 λ0 × 0.75 λ0 × 0.075 λ0, whereas each MTMUCA was sized at 0.312 λ0 × 0.312 λ0, with respect to a free space wavelength of 7.5 GHz. The antenna was fabricated using viscose-wool felt due to its strong metal–polymer adhesion. A naturally available polymer, wool, and a human-made polymer, viscose, that was derived from regenerated cellulose fiber were used in the manufacturing of the adopted viscose-wool felt. The MTMUCA exhibits the characteristics of ENG, with a bandwidth (BW) of 11.68 GHz and an NZRI BW of 8.5 GHz. The MTMUCA was incorporated on the planar monopole to behave as a shunt LC resonator, and its working principles were described using an equivalent circuit. The results indicate a 10 dB impedance fractional bandwidth of 142% (from 2.55 to 15 GHz) in simulations, and 138.84% (from 2.63 to 14.57 GHz) in measurements obtained by the textile UWB antenna. A peak realized gain of 4.84 dBi and 4.4 dBi was achieved in simulations and measurements, respectively. A satisfactory agreement between simulations and experiments was achieved, indicating the potential of the proposed negative index metamaterial-based antenna for microwave applications.

Abstract A tuneable single layer metamaterial (MTM) unit cell comprised of symmetric H-shaped resonators for 5G applications is proposed with epsilon negative (ENG) between 28 - 32 GHz, near zero permeability along the entire frequency range, and near zero refractive index between 28 - 32 GHz. The constructed design offers wide operational frequency range of 24.6 - 31.8 GHz with transmission coefficient resonances (S21) at 28 GHz. The symmetric resonating patch is partitioned into four comparable and equal quartiles, with an H-shaped resonator in each quartile. The metamaterial copper metallic layer is constructed on a Rogers (RT-5880) substrate and has dimensions of 9 × 9 mm2. The scattering parameter for the interaction with the electromagnetic wave is introduced the interesting properties of the effective parameter. The compactness of the subwavelength based metal-dielectric based resonator is related to the tailored metallic design. Numerical simulation of the proposed design is executed in CST microwave studio.

A compact coplanar waveguide (CPW)-fed wideband metamaterial-inspired antenna is designed and developed in this paper. The proposed structure is an asymmetric structure in which three rectangular stubs are employed between signal patch and CPW ground plane. It is demonstrated that these stubs provides lumped parameters of conventional epsilon-negative transmission line (ENG-TL). As an advantage of these stubs proposed antenna operates on 1.67–2.76 GHz with −10 dB impedance bandwidth of 49.2%. The proposed antenna having small electrical size of 0.17λ0 × 0.35λ0 × 0.01λ0 at fo = 2.21 GHz. The simulated average gain and radiation efficiency of the proposed antenna is 1.61 dB and 96.33% respectively throughout the bandwidth. Properties such as smaller electrical size, simpler design, consistent radiation characteristics, and ease of fabrication are making this antenna suitable for GSM, WLAN/Wi-Fi, Bluetooth, and WiMAX applications.

This article presents printed log periodic antennas with metamaterials for use in microwave imaging. A single layer of epsilon negative (ENG) metamaterial (MTM) array (1 × 6) of the unit cell is on the radiating patch. Adding a single negative metamaterial structure enhances the properties of far-field antennas, such as radiation pattern and gain, both of which are vital for breast imaging. Two frequency bands exhibit negative permittivity: 3–3.3 GHz and 3.6–4.5 GHz. In the operating band, the proposed antennas have achieved a maximum gain of 5.5 dBi and impedance bandwidth of 3 GHz (2–5 GHz) with a reflection coefficient less than −10 dB. At the lowest operating frequency of 2 GHz, the electrical dimensions of this designed antenna are 0.34λ × 0.26λ × 0.01λ. All 16 transceiver antennas are arranged vertically in a circular pattern around the phantom, each acting as a transmitter and the rest as receivers. The system design is carried out with the electromagnetic simulators CST and HFSS. After receiving the extracted data, the data are postprocessed using the MATLAB software and the delay multiply and sum (DMAS) imaging algorithm. Based on the reconstructed image, it is evident that the MTM-loaded antenna-based imaging system can detect many undesired tumors inside the breast phantom.

Globalisation and the explosion of communication technologies have created unprecedented challenges for states, with regard to their responsibility for the preservation and maintenance of cultural heritage. In the era of digital content production, state cultural institutions have become key players, as they undertake the tasks of value preservation, value creation and value transfer, and in so doing provide an important public service. Such organisations are responsible for preserving the national character of culture in the face of the negative effects of globalisation, while creating a twenty-first century version of culture that meets the demands of the new media space and social networks.

Social media websites, such as Facebook and Twitter, are starting to become places, where people present and evaluate various events in the world: terrorist attacks in London, Barcelona, Berlin, Brussels. What is more, these websites influence values of their users and readers. Technologies allow people to exchange views at the very moment of the event. The time zone, area, or other physical aspects of the platform participants do not matter. However, this ability might cause negative impact on the discussed social groups. The aim of this article – to analyse the discourse formation in media regarding refugees’ integration and humanitarian crisis in Europe. The goals of this study are: to figure out how the practices of public participation evidences in the context of communication through social media; to form a methodology according to up-to-date communicational concepts and analyse how the images of refugees are formed in social media; to reveal the main actors, involved in the formation of the discourse on refugees in Lithuania, by analysing the content in Facebook pages “Priimsiu pabėgėlį” (eng. “Refugees Welcome”) and “Visuomeninis komitetas prieš priverstinę imigraciją” (eng. “Public Committee against Forced Immigration”).

Negative group delay may be observed in dispersive media with anomalous dispersion in a certain frequency range. The fact that an outgoing wave packet precedes an incoming one does not violate the causality principle but is only a consequence of a waveform reshaping. This effect is observed in media such as photonic crystals, hyperbolic and epsilon-near-zero metamaterials, undersized waveguides, subwavelength apertures, side-by-side prisms, and resonant circuits at various frequencies. The current work is devoted to the design of a simple negative-group-delay medium with tunable properties in the THz frequency range. This medium consists of a bismuth-based frequency-selective surface on a dielectric substrate and may be tuned both statically and dynamically. While a geometry variation defines a main form of an effective permittivity dispersion and group delay/group velocity spectra, an external voltage allows one to adjust them with high precision. For the configuration proposed in this work, all frequency regions with noticeable change in group delay/group velocity lie within atmospheric transparency windows, which are to be used in 6G communications. This medium may be applied to THz photonics for a tunable phase-shift compensation, dispersion management in systems of THz signal modulation, and for encoding in next-generation wireless communication systems.

This paper reports a central spiral split-rectangular-shaped metamaterial absorber surrounded by a polarization-insensitive ring resonator for s-band applications. The rated absorption is 99.9% at 3.1 GHz when using a three-layer structure where the top and ground are made of copper and the center dielectric material is a commonly used FR-4 substrate. The central split gaps have an impact on the unit cell by increasing high absorption, and an adequate electric field is apparent in the outer split ring gap. At 3.1 GHz, the permittivity and permeability are negative and positive, respectively, so the proposed unit cell acts as an epsilon negative (ENG) metamaterial absorber. In a further analysis, Roger4450B was used as a substrate and obtained excellent absorption rates of 99.382%, 99.383%, 99.91%, and 95.17% at 1.44, 3.96, 4.205, and 5.025 GHz, respectively, in the S- and C-band regions. This unit cell acts as a single negative metamaterial (SNG) absorber at all resonance frequencies. The S11 and S21 parameters for FR-4 and Rogers4450B were simulated while keeping the polarization angle (θ and φ) at 15, 30, 45, 60, 75, and 90 degrees to measure, permittivity, permeability, reflective index, absorption, and reflection. The values of the reflective index are near zero. Near-zero reflective indexes (NZRI) are widely used in antenna gain propagation. The unit cell fabricated for the FR-4 substrate attained 99.9% absorption. S-band values in the range of (2–4) GHz can be applied for low-frequency radar detection.

The aim of our studies was to evaluate in vitro methods for the simple and efficient selection of putative probiotic bacterial strains. Of the possible methods, the following were tested: culturing on selective media, Gram staining, catalase assay, hemolytic, clonality and aggregation ability, gastric acid tolerance and bile acid tolerance. A total of 217 bacterial strains isolated from raw sheep’s milk, curdled milk and sheep’s cheese samples produced in Transylvania were included in our experiments. Isolates with hemolytic activity, as well as those exhibiting Gram-negative or catalase-positive phenotypes not characteristic of probiotics were excluded from our studies. Based on the results of RAPD-PCR studies suitable for the detection of individual-level polymorphisms, a total of 34 clone classes and 57 strains with unique RAPD patterns were identified. From each of the 34 clone classes thus narrowed, one strain was selected and tested for its aggregation ability, as well as its gastric acid and bile acid tolerance. High aggregation values above 70%, typical of probiotic strains, were measured in the case of a total of six isolates. In the course of the presence-absence studies conducted on the surface of solid media supplemented with acid or bile acid, it was possible to select several strains specifically tolerant to acid or bile acid. Based on our results, isolates to be included in further tests, e.g., in antibiotic resistance and antimicrobial activity assays, were selected.

Contemporary school should create favorable conditions for schoolchildren. The aim of the study was to comparatively analyze risk factors related to the educational process in different schools and to develop a new approach to objective assessment of combined exposure to environmental factors and factors related to the educational process and its influence on schoolchildren’s health. The study focused on the following research objects: 1) five different secondary schools, the test ones providing profound educational programs and the reference ones being ordinary secondary schools with the same or lower levels of pollution in environmental objects; 2) quality of components in the school environment (organization of the educational process, quality of meals provided by school, quality of ambient air on a school territory and inside a school, quality of drinking water, socioeconomic conditions); 3) health of 756 schoolchildren. The study was conducted by using sanitary-epidemiological, sanitary-hygienic and sociological methods; clinical and laboratory examinations; chemical analytical tests. Fuzzy logic was applied to estimate combined influence exerted by factors related to the educational process and environmental factors. We established several determinants of negative effects produced by the educational process on schoolchildren’s health. They included elevated intensity and monotony of educational and intellectual loads, shorter breaks between classes and recovery index deficiency. Diet-related factors included excess consumption of fats and carbohydrates, overall caloric contents being too high, protein and micronutrient deficiency. Chemical factors were elevated levels of metals, aromatic hydrocarbons, aldehydes, and chlorinated organic compounds in biological media. Risk-inducing factors of schoolchildren, regardless of a school and age, include organization of the educational process (Ipj = 0.45–0.58) and school meals (Ipj = 0.41–0.54); the group potential hazard index for these factors reached its peak values in primary school (Ipj = 0.49–0.58 and Ipj = 0.46–0.54). The maximum value of the integral potential hazard index (Ipdk = 0.41–0.46) caused by combined exposure to factors related to the educational process and environmental factors, regardless of a school type, was detected in senior schoolchildren in school with profound studies of natural sciences (Ipdk = 0.41); the minimum value was detected in a military school (Ipdk = 0.33).

<p class="Abstrak"><em>Coronavirus</em> merupakan salah satu parasit yang menyerang sistem pernapasan manusia. Peningkatan kasus <em>coronavirus</em> berlangsung sangat cepat dan menyebar ke berbagai negara. Oleh karena itu, World Health Organization (WHO) menetapkan <em>Coronavirus</em> sebagai pandemi. Hal ini mengakibatkan seluruh kegiatan yang sebelumnya tatap muka atau luar jaringan (luring) menjadi dalam jaringan (daring), termasuk kegiatan belajar mengajar. Dengan ditetapkannya pembelajaran secara daring menyebabkan adanya opini yang bersifat pro dan kontra dari berbagai kalangan masyarakat. Opini tersebut akan digunakan dalam penelitian ini dan akan diolah terlebih dahulu dalam tahap <em>preprocessing</em>. Metode yang digunakan dalam penelitian ini adalah <em>Longest Common Subsequences</em> (LCS) dan <em>Support Vector Machine</em> (SVM) dengan data sebesar 500 yang terbagi menjadi 250 data berlabel positif dan 250 data berlabel negatif. Dari 500 data tersebut dibagi menjadi 450 data untuk data latih dan 50 data untuk data uji. Dengan menggunakan metode <em>Longest Common Subsequences</em> untuk perbaikan kata dan metode <em>Support Vector Machine</em> untuk klasifikasi dengan nilai parameter terbaik yaitu <em>learning rate</em> (γ) = 0,0001, <em>lambda</em> (λ) = 0,1, <em>complexity</em> (C) = 0,001, <em>epsilon</em> (ϵ) = 0,0001 dan iterasi maksimum = 50 dapat menghasilkan nilai rata-rata hasil evaluasi yaitu <em>precision</em> = 0,5653, <em>recall</em> = 0,948, <em>f-measure</em> = 0,7047 dan <em>accuracy</em> = 0,598. Hasil pengujian tersebut mununjukkan bahwa dengan menambahkan metode <em>Longest Common Subsequences</em> untuk perbaikan kata dapat meningkatkan tingkat akurasi yang sebelumnya hanya 0,59 menjadi 0,598.</p><p class="Abstrak"> </p><p class="Abstrak"><strong>Abstract</strong></p><p class="Abstrak"> <em>Coronavirus is a parasite that attacks the human respiratory system. The increase incases coronavirus took place very fast and spread to various countries. Therefore, the World Health Organization (WHO) has designated Coronavirus as a pandemic. This results in all activities that were previously face-to-face or offline (offline) becoming online (online), including teaching and learning activities. With the establishment of online learning, there are pro and contra opinions from various circles of society. This opinion will be used in this research and will be processed first in the stage preprocessing. The method used in this research is Longest Common Subsequences (LCS) and Support Vector Machine (SVM) with 500 data divided into 250 data labeled positive and 250 data labeled negative. Of the 500 data is divided into 450 data for training data and 50 data for test data. By using the method Longest Common Subsequences for word improvement and the method Support Vector Machine for classification with the best parameter values, namely learning rate (γ) = 0.0001, lambda (λ) = 0.1, complexity (C) = 0.001, epsilon (ϵ ) = 0.0001 and the maximum iteration = 50 can produce the average value of the evaluation results, namely precision = 0.5653, recall = 0.948, f-measure = 0.7047 and accuracy = 0.598. The test results show that by adding method of Longest Common Subsequences for word improvement, it can increase the level of accuracy which was previously only 0.59 to 0.598.</em></p>

The Malaysian government’s move to introduce Jawi-Khat in the Malay-language curriculum in Chinese and Tamil vernacular schools has been fraught with tension and opposition, especially among the Chinese Malaysian community. Being the second-largest ethnic group in Malaysia, the Chinese’s negative response to the initiative has generated some implications for the country’s socio-political order. Sin Chew Daily, the first newspaper to break the news, was accused by then Finance Minister Lim Guan Eng of stirring fears among the Chinese community. Lim’s condemnation of the Chinese daily and the manner in which the whole Jawi-Khat episode played out have raised questions over the roles of Chinese newspapers vis-à-vis their counterparts of other languages in the reportage of the Jawi-Khat move. Thus, a study on the ways three top vernacular-language newspapers in Malaysia, namely, Sin Chew Daily, The Star and Harian Metro, covered this issue, was conducted. The extent of news coverage, news sources, news frames and valence of the reports were analysed. The research findings reveal that each of the newspapers framed the Jawi-Khat controversy differently. Sin Chew remains a classic ethnic newspaper, having reported extensively on the issue and actively pursued the voice of opposition of various stakeholder groups towards a policy that impacts on Chinese education, a key area vital to the Chinese community. Otherwise, the three newspapers have, to varying degrees, performed the interpretive function within a controlled media landscape and attempted to de-escalate conflicts and misunderstanding arising from the Jawi-Khat move. Keywords: Jawi-Khat, media framing, vernacular newspapers, newspaper roles, ethnic relations.

Abstract Among the three classic Philadelphia-negative myeloproliferative neoplasms (Phneg MPN), myelofibrosis (MF) is the most severe in terms of survival and quality of life, with very limited therapeutic options. Although the pathogenesis of Phneg MPN remains still poorly understood, aberrant megakaryocytopoiesis is a common, distinctive feature. Specifically, in MF, bone marrow megakaryocytes (MKs) are hyperplastic and show typical morphological abnormalities such as hypolobated nuclei, tendency to form tight clusters and impaired capacity to generate pro-platelets (pro-PLTs) in-vitro. Recent data proved that MF-MK hyperplasia is a consequence of both increased proliferation and reduced apoptosis of MK progenitors, likely correlated to the over-expression of the anti-apoptotic gene Bcl-xL (Ciurea et al. Blood 2007). Protein Kinase Cε (PKCε) is a novel, calcium-independent PKC isoform, capable to modulate cell proliferation, differentiation and survival. Our group showed that PKCε plays a crucial role in normal and malignant hematopoiesis (Mirandola et al. Blood 2006; Gobbi et al. Stem Cells 2007; Gobbi et al. Blood 2009). During in-vitro erythroid and megakaryocytic differentiation of normal CD34+ progenitors, PKCε levels are finely tuned with a virtually opposite kinetic: progressively increasing during erythroid maturation while peaking early and then decreasing during MK maturation. Forced expression of PKCε in the later phases of megakaryocytopoiesis delays MK differentiation, proving that PKCε silencing is required for MK full differentiation. Here we investigated the expression of PKCε in primary myelofibrosis (PMF)-MK progenitors and we tested whether PKCε modulation may affect megakaryocytic differentiation of PMF-CD34+ cells. CD34+ cells were immunomagnetically isolated from 5 PMF patients and 2 G-CSF mobilized donors (controls, C) and then cultured up to 14 days in serum-free medium supplemented with 200 ng/mL thrombopoietin, 50 ng/mL Stem Cell Factor and 3 ng/mL Interleukin-3. MK differentiation was assessed by morphological analysis and in-vitro pro-PLT generation. Consistently with current literature, also in our serum-free based culture, PMF-MKs showed impaired differentiation associated with abnormal morphology (smaller size, round and hypolobated nuclei) and reduced pro-PLT generation when compared to C (Fig. 1 panel A). First, we demonstrated by Western Blot analysis (WB), that PMF-CD34+ displayed higher levels of PCKε, phosphorylated PKCε (pPKCε), Bcl-xL and Bcl-2 (Fig. 1, panel B ). Figure 1. Figure 1. This is consistent with their augmented proliferative capacity in MK-differentiating medium [median fold increase of PMF cultures was significantly higher than C (12.91 vs 1.09, respectively, p<0.05)]. Additionally, during in-vitro MK differentiation, PKCε levels of PMF-MKs were significantly higher than C-MKs at any time point of the culture analyzed by WB (a representative picture is shown in Fig. 1, panel C). Consequently, we sought to determine whether PKCε inhibition was able to restore a normal in-vitro MK maturation assessed by: i) MK morphology ii) pro-PLT formation, iii) number of PLTs released in the culture medium (evaluated as number of CD41+/calcein AM+ cells, as described in Gobbi et al. Blood 2013). PKCε activity was pharmacologically modulated in two different experiments by the εV1-2 (CEAVSLKPT) peptide conjugated to TAT47-57 (CYGRKKRRQRRR) by a cysteine disulfide bound (Brandman J. Biol. Chem. 2007). As shown in Fig. 2, treatment with εV1-2 was capable to restore a normal MK morphology (panel A) and adequate pro-PLT formation (panel B). Addition of the sole vehicle (TAT47-57) in the culture medium did not provide any improvement on cell maturation, proving that the effects we observed were entirely attributable to PKCε-inhibition by εV1-2. Figure 2. Figure 2. Additionally, a clear trend in terms of increase of the number of PLTs released in the media of PMF cultures treated with εV1-2 was shown (Fig. 2, panel C). Our data demonstrate for the first time a potential involvement of PKCε in the pathogenesis of MF and that PKCε inhibition may revert, in-vitro, the abnormal megakaryocytopoiesis that typifies this neoplasm. Since PKC and PKCε are currently under investigational use in a number of diseases, PKCε inhibition may configure as a new potential therapeutic strategy for MF patients. Disclosures: No relevant conflicts of interest to declare.

<p class="Abstrak">Adanya virus baru yaitu COVID-19 atau SARS-CoV-2 yang berasal dari Wuhan, China pada awal tahun 2020 telah menggemparkan seluruh warga dunia salah satunya Indonesia dan memiliki tingkat penularan yang tinggi. Sehingga untuk meminimalisir penyebaran COVID-19, pemerintah Indonesia menetapkan salah satu kebijakan dalam dunia pendidikan yaitu pembelajaran/perkuliah <em>online</em>. Kebijakan tersebut mengakibatkan seluruh penyebaran informasi berubah menjadi online dan memberikan dampak yang luas bagi keberlanjutan pendidikan di Indonesia. Hal tersebut menimbulkan kontroversi pada kalangan masyarakat dan banyak yang akhirnya beropini pada media sosial, salah satunya Twitter. Analisis sentimen berguna untuk mengetahui ketepatan komputasi sistem dalam mengenali pembicaraan pada Twitter mengenai kebijakan pembelajaran <em>online </em>cenderung bersentimen negatif atau positif dengan menggunakan metode <em>Support Vector Machine </em>dan <em>Lexicon Based Features. </em>Penggunaan <em>Lexicon Based Features </em>berpengaruh terhadap objek penelitian yang menghasilkan nilai<em> accuracy </em>0,6<em>, </em>nilai<em> precision </em>0,56<em>, </em>nilai <em>recall </em>0,75<em>, </em>dan<em> fmeasure </em>0,64 dengan parameter optimal dalam mencapai konvergensi yaitu 𝜆 (Lambda) = 0,7, nilai parameter 𝛾 (gamma) = 0,0001, nilai parameter 𝐶 (Kompleksitas) = 0,0001, iterasi = 50, dan 𝜀 (Epsilon) = 0,00000001. Hal tersebut menunjukkan bahwa metode yang digunakan pada penelitian ini dapat mengenali pembicaraan data komentar pada Twitter karena dibuktikan dengan nilai <em>accuracy </em>yang cukup tinggi.</p><p class="Abstrak"><strong><em>Abstract</em></strong></p><p class="Judul2"><em>The existence of a new virus, namely COVID-19 or SARS-CoV-2, that come from Wuhan, China, in early 2020, has shocked all citizens of the world, including Indonesia, and has a high transmission rate. So to minimize the spread of COVID-19, the Indonesian government has set one policy in online learning/lectures. This policy resulted in all information dissemination being online and had a broad impact on education in Indonesia. This policy caused controversy among the public, and many ended up giving opinions on social media, one of which was Twitter. Sentiment analysis is useful for determining the timeliness of system computing in discussions on Twitter regarding online learning policies that tend to have negative or positive sentiments using the Support Vector Machine and Lexicon Based Features methods. The use of Lexicon Based Features affects the object of research which produces an accuracy value of 0.6, a precision value of 0.56, a recall value of 0.75, and a size of 0.64 with the optimal parameter in achieving convergence, namely (Lambda) = 0.7, the parameter value (gamma) = 0.0001, the parameter value (Complexity) = 0.0001, iterations = 50, and (Epsilon) = 0.00000001. This evaluation value shows that the method used in this study can recognize the conversation of comment data on Twitter because a fairly high accuracy value evidences it.</em></p>

In Germany, the discourse about the incomerelated parental leave policy introduced in 2007 and its legitimisation strongly depends on its effects in the context of certain goals. However, the question of which goals the parental leave policy actually has, is answered controversially in the arenas of politics, media and science. This article shows the characteristics of the different discourses about the parental leave policy in these arenas and the mechanisms behind their interaction. Empirically, frequency analyses and valency analyses of newspapers between 2004 and 2012 are carried out which are combined with content analyses of documents of the German Federal Parliament. The analyses confirm that the different goals can be categorised into five groups. The results show that the definition of goals is the key for understanding the discourses and the public judgement of the parental leave policy. OLS regressions show that the main factors for a positive or negative disposition in the media are the initial reason for the article and the number of considered goals. Hence, for politicians, a communication strategy stressing all five goals is promising. Zusammenfassung Der Diskurs über das 2007 eingeführte Elterngeld und dessen politische Legitimation hängen eng mit der Wirkung auf bestimmte Ziele zusammen. Welche primären Ziele das Elterngeld hat, wird in den Sphären Politik, Medien und Wissenschaft unterschiedlich beantwortet. Der Beitrag zeigt die jeweils charakteristischen Merkmale der Elterngeld-Diskurse in diesen Bereichen und durch welche Mechanismen diese wechselseitig verknüpft sind. Empirische Grundlage ist eine Frequenzund Valenzanalyse von Zeitungsartikeln für 2004 bis 2012, die mit einer Inhaltsanalyse parlamentarischer Dokumente kombiniert wird. Die Analysen belegen, dass das Elterngeld in allen drei Sphären fünf Zieldimensionen hat. Sie verdeutlichen, dass die Definition von Zielen des Elterngelds der Schlüssel zum Verständnis der Diskurse und der Bewertung des Bundeselterngeld- und Elternzeitgesetzes (BEEG) in der Öffentlichkeit ist. Basierend auf Regressionsanalysen sind der Anlass der Berichterstattung und die Summe der attribuierten Ziele zentrale Faktoren für die Valenz von Zeitungsartikeln. Demnach ist für die Politik eine Kommunikationsstrategie vielversprechend, die den Fünfklang an Zielen betont.

Abstract Human C/EBPε is expressed as four isoforms (32, 30, 27, 14kD) through alternative promoter usage, RNA splicing, and translational start sites. We are studying the C/EBPε isoforms in vivo in cord blood (CB) CD34+ progenitors to define their roles in granulocyte differentiation and hematopoietic lineage specification. The C/EBPε32/30 isoforms function as transcriptional activators. We reported that C/EBPε27 interacts with and is a repressor of GATA-1 transactivation of eosinophil-specific genes (Du J et al. JBC2002; 277:43481). C/EBPε14, which contains DNA binding and bZIP domains but lacks a transactivation domain, may function as a dominant negative repressor of C/EBPε32,30 or other C/EBPs through heterodimerization or competition for C/EBP binding sites. We assessed mRNA expression of the C/EBPε isoforms during eosinophil differentiation of CB CD34+ progenitors to determine their temporal patterns of expression. CD34+ progenitors (&gt;95% pure) induced to differentiate into eosinophils by SCF, IL-3, and IL-5 showed differentiation to &gt;90% eosinophils by 17–19 days. Semi-quantitative RT-PCR using C/EBPε isoform-specific primers showed that CD34+ progenitors initially express only the repressor C/EBPε14 isoform, with induction of all isoforms by day 3 to different peak levels of expression by days 9–11 during the promyelocyte to myelocyte transition. These results define temporal changes in the expression ratios of the C/EBPε activator vs. repressor isoforms during eosinophil development that may differentially regulate gene transcription in this process. We subcloned the C/EBPε isoform cDNAs into an MSCV-based bicistronic retroviral vector (pGCDNsam IRES-EGFP) and ectopic expression was induced in CB CD34+ progenitors by retroviral transduction for 72hrs. The CD34+/GFP+ cells were sorted by FACS and plated in Collagen Cult™ media containing SCF, IL-3 and a lineage-specific cytokine (i.e. EPO, G-CSF, or IL-5), or in suspension culture containing SCF, IL-3 and IL-5 to drive eosinophil differentiation. Total and differential colony and cell counts were performed after 15–17 days based on colony morphology, histochemical and enzyme staining of the cells. The activator C/EBPε32 isoform significantly altered myeloid development, favoring eosinophil over neutrophil or erythroid development. Even in cultures containing EPO, cells transduced with C/EBPε32 failed to develop into erythroid colonies (BFU-E). C/EBPε27, a potent repressor of GATA-1 in vitro, inhibited erythroid colony growth by ~50%, and doubled the numbers of granulocyte-macrophage colonies compared to C/EBPε14 or empty vector control. C/EBPε32 strongly induced eosinophil colony formation at the expense of neutrophil other myeloid lineages regardless of the cytokines used, inducing ~90% eosinophil colonies. C/EBPε27 reduced eosinophil colonies by &gt;50%. Likewise, &gt;90% of cells transduced with C/EBPε32 and grown in suspension culture were eosinophils, whereas C/EBPε27 and C/EBPε14 inhibited eosinophil differentiation by ~50% and &gt;98%, respectively. Thus, the C/EBPε isoforms: (1) are differentially expressed during eosinophil development, (2) have the capacity to reprogram stem cells to different myeloid lineages consistent with their predicted activator vs. repressor activities and interactions with hematopoietic transcription factors such as GATA-1 or other C/EBPs, and (3) may be useful in their ability to reprogram myeloid terminal differentiation for the development of novel approaches to treat myeloid or other leukemias.

The aim of this project was to evaluate the effects of zinc in osteogenic media and its effect on the differentiation of adipose-derived stem cells (ASC) into osteoblasts. Zinc has a stimulatory effect on bone formation and mineralization in vivo and vitro (Seo et al. 2010 Nutr. Res. Pract. 4, 356–361). Our hypothesis was that the presence of zinc in the osteogenic media would positively influence both the speed of formation and the number of osteoblastic nodules formed. Swine ASC were isolated as described (Monaco et al. 2009 Open Tissue Eng. Regen. Med. J. 2, 20–33). The ASC were divided in 8 different treatments: 6 different concentrations of zinc in the osteogenic medium (8, 4, 0.8, 0.4, 0.08, and 0.04 mM) plus 2 control treatments (osteogenic medium without zinc and a negative control, DMEM). The media was changed twice a week for 4 weeks. The experiment was replicated 4 times. At the end of the culture period, cells were stained with Alizarin Red S. In each well, we counted the nodules and divided them in 2 categories: formed and forming nodules. The second evaluation that we did was to evaluate the diameter of the largest nodules (2/well) in each group. Data were analysed by ANOVA using the Generalized Linear Model procedure (SPSS, IBM Corp., Armonk, NY, USA). Bonferroni’s post-hoc test was used to perform statistical multiple comparison. The α-level was set at 0.05. The results showed that the doses of zinc of both 4 and 8 mM were toxic to the whole cell populations in this treatment, which was indicated by cell death, whereas the concentrations of 0.8 and 0.4 mM were not cytotoxic but no nodules formed. Here we report the results that are greater than zero in Table 1. There is a positive effect on nodule formation when the zinc is added to the media. It is clear that the total number of nodules is different between the 0.08 mM zinc group and the control (P < 0.003). When we evaluated nodule diameter we found a direct correlation between the zinc concentration and the diameter of the nodules: 292.7 (±136.6) v. 366.8 (±218.7) v. 423.7 (±267.7) µm for the control, 0.04 mM zinc, and 0.08 mM zinc, respectively. The largest nodule was found in the 0.08 mM zinc treatment at 886.6 µm. These results confirmed the positive effect of this mineral on bone formation. This preliminary experiment is the first step towards the analysis of the behaviour of ASC on scaffolds with zinc incorporated into their matrix. Table 1. The average number (SD in parentheses) of formed and forming osteoblast nodules compared between treatment groups

Tissue engineering offers a viable alternative to bone grafts in repairing large bone defects. This involves using scaffolds of various sizes and shapes that contain stem cells and other osteoinductive molecules. The aim of this project was to evaluate the effects of vitamin K in osteogenic medium and its effect on the differentiation of adipose-derived stem cells (ASC) into osteoblasts. Vitamin K has been shown to increase bone mineral density by acting as a coenzyme in the γ-carboxylation of osteocalcin, a protein involved in bone mineralization (Weber 2001 Nutrition 11–12, 1024). Our hypothesis was that the presence of vitamin K in the osteogenic medium would positively influence the number of osteoblastic nodules formed. Swine ASC were isolated as described (Monaco et al. 2009 Open Tissue Eng. Regen. Med. J. 2, 20–33). The ASC were divided into 7 different treatments: 5 concentrations of vitamin K in the osteogenic medium (10, 50 100, 500, 1000 nM) plus 2 control treatments (osteogenic medium without vitamin K and a negative control, DMEM). The media was changed twice a week for 4 weeks. The experiment was replicated 6 times. At the end of the culture period, cells were stained with Alizarin Red S and Von Kossa. In each well, we counted the nodules and then divided them in 2 categories: formed and forming nodules. Data were analysed by analysis of variance using the generalized linear model (GLM) procedure of SPSS (IBM/SPSS, Armonk, NY, USA); the least significant difference (l.s.d.) post hoc test was used to perform statistical multiple comparison, and the α-level was set at 0.05. The results showed (in Table 1) that there was no positive effect on nodule formation when vitamin K was added to the medium; however, when 1000 nM vitamin K was added, nodule formation decreased. More experiments need to be conducted to determine if vitamin K can act synergistically with other vitamins to produce a significant role in ASC differentiation into osteoblasts. This preliminary experiment is the first step towards the analysis of the behaviour of ASC on scaffolds with vitamin K incorporated into their matrix. Table 1.The average number of formed and forming osteoblast nodules compared between treatment groups (SD in parentheses)

Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane). Biosurfactants can reduce the stress between solids and liquids on the surface and at the end. Biosurfactants have several properties, i.e. they are stable, less harmful, as well as readily degradable, and extremely eco-friendly. Biosurfactants also have a wide range of industrial uses because they are a versatile category of chemical substances. The principal justification for conducting such research was the isolation of possible biosurfactants containing bacteria. Sampling was performed for the isolation of bacteria producing biosurfactants from different oil-polluted sites That is to say, experiment for emulsification, test for oil spreading, test for drop collapse, and measure for hemolysis. The capability to produce biosurfactants was seen in 22 different isolates from polluted sites B1, B2, and B3. Through different biochemical tests and Gram staining, it was identified that isolated bacterial strains are Pseudomonas spp and that is Pseudomonas aeruginosa. The procedure used as characterizing biosurfactants was the TLC plate’s procedure, by using TLC plates process yellow dots emerged after spraying on silica gel plates with an throne and ninhydrin reagents. These yellow spots confirmed the presence and production of rhamnolipid in the biosurfactant. Hence, it was concluded that identified strains in the study can be helpful in the heavy metals, pesticides, and hydrocarbons bio-degradation and bioremediation. These may also be used as biological control agents to protect plants from various pathogens, resulting in improved crop yields. Introduction Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane) (Chen et al., 2007; Ghayyomiet al., 2012). Main purpose of the bio-surfactantsgeneration or production is a consequence of financial availability (Van Dyke et al., 1993 It is reported that almost 50 percent of the world's surfactants are used because of the need for cleaning agents as well as the rate of growth grows every day (Deleu and Paquot, 2004). Appropriate use of bio-surfactants will control environmental emissions what these are the most dangerous, constantly rising gradually and disrupting the routine maintenance of life every day. Awareness campaign initiatives have been introduced and also increase for environmental laws, various innovative approaches need to be implemented and even the issue of pollution focused entirely. Developing appropriate advanced technologies to help clear up chemicals and toxins from the ecosystem, like hydrocarbons (both inorganic and organic). Studies on biosurfactants are being launched by scholars and researchers with significant health issues like adverse environmental effects, air contamination, environmental change, and waste management (Makkar and Cameotra, 2002 Biosurfactants contribute to expanded demand for such microbial products as alternatives to chemical surfactants (Benatet al., 2000). Microbes seem to have the capability to degrade contaminants, but their biodegradation is limited leading to hydrophobicity, low solubility in water, and inadequate bioavailability, of such pollutants (Patil, et al., 2012). GhayyomiJazeh, Mishraet. al (2001) those bacteria that produce biosurfactants were isolated from the site of petroleum spills and afterward, 160 strains and as well as 59 strains were able to produce biosurfactants have shown better performance in a test for hemolysis of blood, and 45 strains with positive findings within oil spread experiment were applied in the laboratory to isolate and segregate the media cultured Banat process (Rahman et al., 2002) These were observed and researched that biosurfactants of Pseudomonas aeruginosa spp are most likely to disrupt the bonding of hydrocarbons like nonadecane, octa, Hexa, and hepta, in marine Water contaminated with oil spills up To approximately 47%, 53%, 73% and 60%(Abrar et al., 2020). Current study concluded that the isolated strain having the ability to degrade hydrocarbon as well as the ability to degrade the heavy metal. The strain also can protect the plant from various diseases. The present research found that the isolated strain is capable of degrading hydrocarbon while also being capable of degrading the heavy metal. As well as the strain does have the capability to defend plants from different diseases. Material And Methods Area of Study The investigation was conducted at HazaraUniversity(HU) Microbiology Laboratory, MansehraPakistan. Assemblage of Samples Thehomestay area of the city Mansehra Pakistan which is named as a township, where oil spills arose, oil spills soil samples were obtained as well as sampling from various Mansehra automobile workshops were also done. Sterilized bags of polythene were being used to collect samples of the soil, after thatthe sample was taken towards the Hazara University (HU) Mansehra Microbiology Laboratory to examine and extract bacterial strains that could develop biosurfactants. The soil temperature at the time of sample selection was around 30 ° C. The pH was also verified by Galvano science companies at the time of selection by pH meter, and the pH being reported was 7. Preparation of Media 15 x 100 mm Petri dishes were being used to prepare the media. Agar plates were thoroughly cleaned with water from the tap and then carefully covered in aluminum foil following cleaning then placed within autoclave at 121°C for about 15 min at 15 psi for sterilization. The nutrient agar which contains 0.5% NaCl, 0.3% beef extract, 0.5% peptone, and 1.5% agar, in 500 ml of distilled water, 14 g of the nutrient agar media (Merck) were dissolved. The nutrient level used mainly for the production of non-fastidious species. Nutrient agar is widely known as it's capable of growing a variety of bacteria types and provides nutrients required for the growth of bacteria. Upon sufficient dissolution of such nutrient agar in distilled water, these were then sterilized by autoclaving for 15 min at 15 psi in the autoclave and held at 121 °C Upon autoclaving, pouring of the media was done in laminar flow hood, and then packed and placed for yet more use in a fridge at 4°C. 2.4 Preparation of serial dilution The bacteria are isolated using the serial dilution process. During this process, 10 test tubes were taken and distilled water (9ml) was added in each tube. After that tubes were put for 15 minutes in the autoclave machine at 121°C. After that 1gm of a crude oil sample from the soil was added in a test tube containing distilled water. Further, 1 ml of the solution was taken from the first test tube and poured to the adjoining tubes for the preparation dilution as under . Afterward, 10μl of the solution was pipetted from both the dilution of and shifted for spread culture techniques, then incubated the plates at 37°C for 48hrs. Biosurfactants extraction Firstly, in nutrient broth solution theculture of bacteria was added and inoculated with oil, the bacterial colony was then incubated at the temperature of 25°C in a shaking incubator just for 7 days. Incubation after seven days of trembling. Thebacterial Crop was then taken and centrifuged at 5000rpm at temperature 4°C for 20minutes. Following centrifugation, the supernatant was collected and then mixed in the equivalent amount in Methanol: Chloroform. White sediment was then retained and collected for further use . Bacterial Colonies Isolation 1 g of the soil polluted with oil was diluted serially up to 106 dilutions.10 μl of 104 and 106 dilutions for spread culture were transferred to the MSM agar plates and nutrient agar. The plates were then incubated at 37°C for 48hrs. Twenty-two morphologically separate colonies were separated for further specific examination just after the incubation and processed by using the technique of streak plate. Screening of Isolates’ Biosurfactants Behavior To check the activity of biosurfactants produced by the bacterial species the following methods of screening were done. Hemolytic Activity of Biosurfactants for Erythrocytes Blood agar containing 5% of blood was prepared as after the fresh isolates were added and inoculated on blood agar plates, then the plates were taken and placed in the incubator at temperature 37°C for 48hrs (Rashediet al., 2005). Thereafter the observation of clear zone in the colonies indicated the existence of bacterial species that produce biosurfactants. This experiment was undertaken to control the ability of isolated bacteria to induce blood agar hemolysis. Three forms of hemolysis usually involve; alpha, beta, and hemolysis of the gamma. The agar underneath the species is dark greenish, then it is Alpha, the yellowish color produced in beta hemolysis and gamma hemolysis does not affect the bacterial sppwhichadded on the plates (Anandaraj and Thivakaran, 2010). Bio-surfactant identification with process of CTAB MSM (Mineral salt agar medium) with (2%) of glucose serving both as carbon source, (0.5 mg / ml) acetyl-tri-methyl-ammonium-bromide (CTAB), and methylene blue (MB: 0.2 mg/ml) are used to detect anionic bio-surfactants (Satpute et al., 2008). For this method, thirty microliters (30μl) of cell-free supernatant were added to each of the wells of the methylene blue agar plate that comprises of borer (4 mm in diameter). after that, the incubation of the plates was done for 48-72 hrs at 37°C. Just after incubation in each of the wells, a dark blue halo zone was being used to show the successful anionic bio-surfactant production. Table 1: Composition of MSM Media S. No Ingredients Amount (gm/L) I Potassium dihydrogen phosphate (KH2PO4) II Magnesium Sulfate (MgSO4) III Iron Sulfate (FeSO4) IV Sodium Nitrate (NaNO3) V Calcium Chloride (CaCl2) VI Ammonium Sulfate (NH4)2SO4 Technique for Spreading of Oil A sufficient number of isolated bacteria were inoculated into a solution of 100ml nutrient broth. Over 3 days, the culture was incubated at 37 ° C in a rotating shaker incubator (150 rpm). After that biosurfactants synthesis was checked in culture suspensions (Priya and Usharani, 2009; Anandaraj and Thivakaran, 2010). For this process, thirty milliliters (30ml) of distilled water was added in a Petri dish. In the middle of the distilled water, 1 milliliter (1ml) of diesel oil was added, and then a centrifuged twenty microliter (20μl) culture was introduced to the middle of a plate, which was isolated from oil spilled soil or local oily groundwater. The species producing the bio-surfactant displace the hydrocarbons and disperse it even in the water. Then it was calculated and analyzed within 1 mint (Ali et al., 2013). Technique for Drop collapse In this process, 96-wellsformed in each of the plates of nutrient agar. Afterwards, all the 96-wells of microliter plates was then filled withmineral oil of about 2ml. Then stabilized the plate at 37oC for 1 hour, after which the oil surface was filled with 5μl of supernatant culture. Therefore, the drop shape was taken to be observed on the oil surface after 1min. The drop which was collapsed, generated by the supernatant culture which is used to signify positive(+ive) outcome and the drops which stayed the same and displayed no changeindicates negative(-ive) outcome. And was taking distilled water as a control(Plaza et al., 2006). Emulsification index The emulsification index was calculated, as stated by the process followed by Cooper and Goldenberg (1981) In this process, 2 ml of kerosene oil was taken and inserted in each of the test tubes to the same amount of cell-free supernatant, and then homogenized for 2 min in a vortex at high speed and allowed for 24 hours to stand. The emulsification steadiness was then determined after the 24 hours, and the emulsification value was estimated by measuring the emulsified layer height by the total liquid layer height, then multiplied by 100. Quantification for the Dry weight of Biosurfactants The bacterial colony was inserted and inoculated in the nutrient broth medium, followed by oil and centrifuged at 5000rpm and after that, the supernatant was clutched and treated with chloroform and methanol and mixed. The white colored deposits were taken and used for the furtherprocess of dry weight. Afterwards, took the clean Petri plate and determined the empty plate weight. Next, the sediment was poured onto Petri plates. Now, for the drying process the hot air oven was used and set the 100ºC of temperature for 30minutes and the plates were put in the oven. After the drying process, the plates were weighted again. The dry weight was calculated for the biosurfactants using the formula which described below: Selected strains Identification and their characterization Instead, various basic biochemical methods were used to identify the isolated bacterial strains. Various biochemical tests, such as Gram staining, Oxidase test, Urease test.Catalase test, Methyl red test, Motility test, Indole test, Starch hydrolysis, Citrate test, Spore staining, Gelatin hydrolysis. Then afterwards, for the preliminary characterization of the biosurfactant, the thin layer chromatography process was used. Physical characterization of the strains selected Gram staining First, on the slide, using the wire loop the bacterial pure culture was taken, and smear was prepared on the slide, and then a drop of purified water was applied. Then, the sterile loop or needle was correctly mixed the bacterial colony and purified water, then mixed up until it is somewhat turbid. Then, spirit lamp was used to fixed the bacterial smear on slide and cooled to room temperature. With this glass slide was loaded with solution of crystal violet and stood for 1minute anddistilled water was applied on slide. Meanwhile the slide was submerged for 1 minute with the iodine solution, and then flushed and rinsed with water. Therefore, decolorizer of about 1 to 2 drops(5 percent acetone and 95 percent alcohol) were added to the slide’s smear and stand for 30seconds, and then treated with water. After then slide was rinsed with safranin for 60seconds, and then treated with water anddry in air. Microscopic analysis was done with 100x objective lenses using emersion oil on smear. Cell morphology The isolates of the bacterial cell were gram stained on slides and then the slides were observed under the light microscope, showing the shape and color of the cells. Biochemical characterization of the selected strains Catalase test Aim of this study is to identify, evaluate and examine that, whether or not the microbes are capable of producing catalase enzymes, while catalase is a protective enzyme, i.e. catalase has the potential to protect against the lethal chemicals known as (H2O2). In this study a bacterial culture that was clarified overnight was used. This culture has been smeared on a glass slide, and 3 percent hydrogen peroxide (H2O2) has been applied and observed on smear. Effects have been observed for bubble formation. Citrate test This study was performed to check the amount or ingest the citrate as the carbon and energy supply for growth and metabolism. Medium containing bromothymol blue and sodium citrate as pH indicator, bacterial was introduced. Ammonium chloride is also present in this medium used as a nitrogen source. Results were noted with variations of color from green to blue. Urease test The capability of urease enzyme for degrading urea was calculated in this bacterial capacity test. Bacterial culture was taken and inoculated for 48 hours at 37 ° C in urease broth, and then color was observed. Methyl red test Through using the process known as mixed acid fermentation which is used to evaluate the bacteria's acid production. The bacterial culture was taken and introduced in the broth of MR-VP and then incubated for 3days at a temperature of 37°C. Two (2) to three (3) drops of Methyl red were added in the broth medium after the incubation period. The change in broth color was observed for final results after a few seconds. Indole test Through using the process to assess the bacteria 's capability to crash indole from tryptophane molecules. After the 24 hours of incubated, taken the fresh inoculum of bacteria and then inserted into the tryptone medium, 24 hours of incubation of about 30oC, 2ml of the tryptone broth medium was added into a sterile test tube. Kovac's reagent was taken to be added (few drops) in sterile test tube and stimulated for a few minutes, and variations of color were detected. Gelatin test It is the approach assess to figure out the use of enzymes known as gelatins from bacterial organisms that precipitate the gelatin. Fresh inoculum of bacteria was taken after 24 hours, and inserted into the media of gelatin agar. This was incubated for around 24 hours, so the temperature did not exceed 30 ° C. Media was observed after incubation time. Starch hydrolysis Several of the micro-organisms that use the starch as a carbon energysource. Therefore, this method has been used to assess whether or not bacteria may use starch as a source of carbon. The bacterial fresh inoculum was spread on the petri starch agar plates, and after that the plate was incubated for 24 hours andmaintained the temperature at 30 to 35 ° C, then gradually applying the supplements of iodine to the plates to flow the change, and then examining the plates. Preliminary characterization of the strains selected Experimental characterization of the bio-surfactant was performed by using the process of TLC (Anandaraj et al., 2010). On a silica gel plate, crude portion of the rudimentary bio-surfactant was separated using Methanol: Chloroform: water (CH3OH: CHCl3: H2O) in the ratio of as an eluent with a different color producing reagents. Ninhydrin reagent (0.5 g ninhydrin in 100ml anhydrous acetone) was used to find bio-surfactant lipopeptide as red spots and anthrone reagent (1 g anthrone in 5ml sulfuric acid combined with 95ml ethanol) as yellow spots to identify rhamnolipid bio-surfactant (Yin et al., 2008). Results and Discussion Isolation of bacteria At first, twenty-two (22) strains from a polluted soil sample were isolated from nutrient agar media.Mixed culture provided by these colonies, so they were taken and smeared on the plates of nutrient agar and then fresh inoculum was collected and stored at temperature of 4oC for the further analysis. Bio-surfactants (surface-active compounds)are formed by a variety of amphiphilic bacterial and fungal organisms that are extracellular (a part of the cellular membrane) (Chen et al., 2007). Screening of Isolated strains for biosurfactant producing colonies Different experiments were carried out to identify, isolate and screen bacteria that are capable of generating bio-surfactants and that is Oil spreading technique(OST), blood hemolysis test(BHT), CTAB test, Emulsification operation. There were twenty-two distinct isolates observed in the current research. And the B1, B2 and B3culture were taken and selected from the twenty-two (22) strains isolated from the polluted spot, which were found to produce biosurfactant. And the oil spreading technique showed promising results for these strains. And strain B2 showed a greater displacement of oil and this is 4 mm. Oil spreading method is quick and often easy to handle, and this technique requires no particular equipment, only a very small amount of sample is used. This approach can be applied when the production and quantity of biosurfactant is small (Plaza et al., 2006) and (Youssef et al., 2004) Only bacterial cultures have been allocated and screened for bacterial species that can generate or use biosurfactants. Just three (3) strainsamong them presented the best results.Those 3 strain,s (B1, B2 and B3) were selected as an additional analysis. Blood hemolysis test On the petri plates of blood agar, the . Isolated bacteriaof B1, B2 and B3 were taken andstreak at the temperature about 37°C for 48 hours. Strain B1 demonstrated β (Beta) hemolysis after the incubation cycle and B2 and B3strains demonstrated γ (Gamma) hemolysis. The B1 strain had an emulsification index of about 74 percent and that was very high as compared to 70 percent for B2 and about 53 percent for B3 respectively. Around the same time, B1 strain showed β (Beta) hemolysis and γ (Gamma) hemolysis was shown bystrains B2 and B3 on the platesof bloodagar. The β hemolysisshowed by the strain B1 in the blood agar test, and the strain B2 and B3 showed γ (Gamma) hemolysis. It is determined that 20 percent strains that are the bestproducer of rhamnolipid have not fully lysed the blood, because the ability of the producer strains capacity not be responsible for the hemolytic activity. According to many researchers, who have shown that this is not such an effective tool for biosurfactant detection due to many bioproducts that may also induce red blood cell lysis, that is not so sufficient to be the surface-active molecule (Youssef et al., 2004). (Rashedi and others, 2005). Table2 Blood Hemolysis Test CTAB agar plate test This test confirms the anionic biosurfactants development. After plate incubation at a temperature of 37 ° C for 72 hours, dark blue hollow zone was existedaround each of the B1 strains wells, which clearly indicated the positive (+ive) development of anionic Biofactant. In addition, the B1 and B2 strains showed positive (+ I ve) results and, in the CTAB analysis, the B3 strain was found to be negative (-ive). The growing microorganisms when secreted the anionic biosurfactants on the plates of CTAB (cetyl-tri-methyl-ammonium-bromide) and methylene blue, then as a result the dark blue-purple insoluble ion pairs formed on the plates. The halo zone around each of the colonies was developed that can recognize rhamnolipid production and that was dark blue in colour, and could correlate with production of rhamnolipid (Siegmund et al., 1991). As indicated in (Fig1) Fig1: B1 positive on CTAB agar plate Oil Spreading Technique The oil was displaced by B1, B2and B3 strains in this test strain and showed a zone that was so clear. The bacterial strains capable of developing biosurfactant were tested and separated from the sample of soil which was oil spilled and brought from the District of Mansehra, Pakistan and from automobile workshops of Mansehra. As shown in (Fig.2). Fig.2: Results of Oil Spreading by B1, B2 and B3Table 3;.Test for oil spreads Bacterial culture Formation of zone (mm) Readings B,1 B,2 B,3 Drop-collapse technique During this process the drop shape was observed at the oil surface. As seen in Fig 3, the collapsed drop was provided by the supernatant culture B1 , B2 and B3.. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Fig 3: Result of Drop-collapse test Table 4: The activity of Biosurfactant emulsification Dry weight of bio-surfactants In this examination, white-colored sediment was collected. Then measured the weight of the sterile Petri plate which was empty in the first step. Then, the sediment was poured into plates. The plates were taken and weighted after 30 minutes of drying on a hot air oven, following the process of drying. The weight of biosurfactants (dry weight) was measured using the following formulations: Fig 4: Dry weight of biosurfactants Table: 5: Dry weight of the biosurfactants Bacterial Culture Weight of the plate (g) biosurfactant in The plate after drying (g) Dry weight of Biosurfactant (g) B,1 B,2 B,3 Identification of selected strains and their characterization Gram staining For structural applications, and stroke analysis gram staining method was used.(Fig.5) shows findings from the process of gram staining. Fig 5: Microscopic view of Gram staining Biochemical identification of bacterial strains and their characterization Specific biochemical studies were performed to identify the species for further recognition and characterization. The bio-surfactant producing microorganism was found to be Pseudomonas aeruginosa after conducting various characterizations and the biochemical tests(Eric Deziel et al., 1996), Which can be used to further analyze and study the industrial development of the biosurfactant. Rhamnolipid is also isolated and produced from the Pseudomonas aeruginosa species on the silica gel plate (Rashedi et al., 2005), a form of biosurfactants highly recommended for processes of bioremediation. All the findings collected from biochemical testing were labeled as Berge 's Manual and it revealed that the protected microorganism was (Pseudomonas aeruginosa). Results of biochemical test were tabulated in (Table.5) Table 6: Bacterial strain identification Tests B1 B2 B3 Gram staining Negative Negative Negative Oxidases Positive e Positive Positive Catalase Positive Positive Positive Indole Positive Negative Negative Citrate Positive Negative Negative Urease Negative Positive Negative Nitrate Positive Positive Positive Motility Positive Positive Positive Gelatin hydrolysis Positive Negative Negative Lactose Negative Positive Positive Methyl red Negative Positive Positive Voges Proskauer Negative Negative Negative Fig 6: Results of biochemical tests(A) Methyl red and Voges Proskauer tests (b) catalase tests (c) oxidase tests (d) indole tests (e) citrate tests (g) lactose tests (h) urease tests Preliminary bacterial strain’s characterization The plates showed yellow dots, when sprayed with anthrone reagent. It indicated the existence of biosurfactants of rhamnolipid in the organism on the plate of TLC as seen in theFig.7 Fig 7: Biosurfactant characterization by TLC Conclusion Biosurfactant development is exciting and perceptible across industries to clean up oil waste and pollutants, particularly in the ecosystem.Compared with chemical surfactants, the biosurfactants are less harmful. It plays an important role in defining the advantages and the importance of industrial applications. Therefore, it is not possible to disregard the growing role and importance of biosurfactants in environmental sustainability.Biosurfactant formulations which can be used for bacterial, fungal, and viral organisms as growth inhibitors. Such biosurfactant inhibition properties can make them components that are applicable to Numerous illnesses that are used as medicinal agents. Therefore it was decided that the described strain could be used as a potential source for heavy metal bioremediation pesticide and hydrocarbon polluted sites. And also used as shielding the plant from different pathogens, contributing to improved crop yields. There is no doubt that the biosurfactants are a multifunctional, advanced, versatile, long-lasting and updated type not only for the twenty-first century but beyond. Conflict of interest The authors declared that they have no conflict of interest and the paper presents their own work which does not been infringe any third-party rights, especially authorship of any part of the article is an original contribution, not published before and not being under consideration for publication elsewhere. References Ali, S.R.; Chowdhury, B.R.; Mondal, P. and Rajak, S. “Screening and characterization of biosurfactants producing microorganism from natural environment (Whey spilled soil)”. Nat. Sci. Res. 2013, 3(13), 34–64. 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The quantity of municipal solid waste (MSW) generation is escalating at an alarming rate with every passing year alongside the modernization of our economy. Unfortunately, the majority of this waste remains uncollected or ends up in open dumping and followed by uncontrolled burning. Citing the deep-rooted consequences, open dumping should be absolutely abandoned and scientific interventions should be aggressively exercised to reclaim the municipal brownfields. The present research work undertook the judicial task of assessing the comparative feasibility of biomining and scientific capping as a technology selection for reclamation of about a decade old 120 million tons of waste chunk laying at Jawahar Nagar dump yard. Primary dump samples were collected from various locations, considering depth as a variable. While leachate and groundwater samples were collected from Malkaram lake and preinstalled borewells receptively. Additionally, the ambient air quality and noise level also been ascertained within the buffer zone. The blended representative solid sample was segregated using a 70 mm mesh size trommel into organic and inorganic fractions. The organic fraction was composted using a lab-scale aerobic static pile composting (ASPC) while the trommel reject was processed as refuse derived fuel (RDF). Evidently, the compost lagged quality and depicted nutrient deficiency. While the burning of RDF produced siloxane gas, significantly due to elevated silicon level in the primary waste. Furthermore, due to the prolonged leaching tenure and seasonal dilution, the concentration of legacy leachate was relatively weaker. Borewell samples collected from a depth of 20 feet also portrayed minor contamination up to 500 meters horizontal radius. The issue of leachability can solely be resolved with the capping of the existing dump and the end product quality derived from the biomining process is highly questionable. Thus, handling such large quantity capping is a befitting option over biomining for Jawahar Nagar dumpsite. INTRODUCTION Presently, in India due to rapid urbanization and industrialization, the generation of MSW has been increasing tremendously and also expected to continue a similar trend in the future (Scott, 1995; Bhat et al., 2017; Sethurajan et al., 2018; Sharma et al., 2018). Annually, the comprehensive urban MSW generation in India is more than 62 million tons. Metro cities are the mammoth contributor of the entire chunk and waste production had already reached an alarming figure of 50,000 tonnes/day. While the waste generation from the tier 2 cities is also rigorously escalating and presently contribute up to 20,000 tones/day (Sharma et al., 2018). A study conducted by the central pollution control board (CPCB) revealed MSW generation in India is increasing at a distressing rate of 5 % per annum with a sharp escalation in the quantities of domestic hazardous waste (Sharma et al., 2018). With major financial constraints, inefficacy of collection, treatment, and disposal incurs further reasons to worry. So far India has miserably failed to set up wholesome source segregation and collection method. Presently, the country spends more than 60% of its annual waste management budget only in collection. Besides, only 20% or less of the collected materials are scientifically handled and treated. Citing the statistics, it is evident that the majority of the MSW is simply gets dumped on the low laying grounds located somewhere on the outskirts of the cities. The precipitation, infiltration, surface water runoff, bird menace, rodent interference etc. triggers the vulnerability of waste and leads to mal odor, ground and surface water contamination, human and environmental health deterioration (Jayawardhana et al., 2016). Further, the perseverance of the inorganic and inert fractions leads to soil contamination, poses a fire threat, and also may incur carcinogenicity and acute toxicity among the animals (Mir et al., 2021). There are numerous techniques for the reclamation and remediation of the dumpsites, includes processes such as capping and closure, in-situ vitrification, sub-surface cut-off walls, and waste biomining (Chakrabarti and Dubey, 2015; Thakare and Nandi, 2016). Waste biomining is a stable way to get rid of the entire range of problems associated with open dumping and reclaim valuable land (Kaksonen et al., 2017). There are several instances including reclamation of Mumbai Gorai dump yard by IL & FS Environment, 70 – 80 years old 12,00,000 tons of dump clearance by Nagar Nigam Indore within a minute span of 3 years and many more. But the process of biomining is highly sensitive and case-specific. The success of the process solely depends on factors such as characteristics of the waste, efficacy of the effective microorganism culture, acceptability of the processed end product at the local market etc. (Jerez, 2017; Banerjee et al., 2017; Venkiteela, 2020). Contrarily, though the scientific capping is not an end-to-end solution but still advisable in the cases where the quantity of waste is gigantic, land scarcity is prevalent, no nearby industries to consume the end products etc. Mehta et al. (2018) have also supported the above claim based on the assessment of locations specific MSW dump reclamation case studies. While in another Nagpur-based case study conducted by Ashootosh et al. (2020) reported the superiority of the biominingprocess over simple land capping due to the favorability of the local conditions. Capping eliminates the environmental interference and thereby reduces biosphere contamination and leachate generation. Further, it captivates rodent and vector breeding and thereby curtails the spreading of communicable diseases and improves aesthetics. But right consolidation through compaction and execution is utmost necessary in the above case. As non-compaction and faulty sloping will easily lead to heavy settlement and slope failure (Berkun et al., 2005; Al-Ghouti et al., 2021). The present study has been pursued with the primary objective to run a techno-commercial assessment between scientific capping and biomining. While the secondary objective was to ascertain the level of contamination and propose mitigative measures. MATERIALS AND METHODStudy Area Spanning over 350 acres of a precious piece of land at the outskirts of Hyderabad city, Jawahar Nagar dumping yard was brutally utilized by the Greater Hyderabad Municipal Corporation (GHMC) for open dumping for a prolonged tenure of 10 years. It housed nearly 12 lakh metric tons of heterogeneous solid and domestic hazardous waste and continues polluting until 2015, until the Ramky group was offered to cap the legacy dumping and scientifically handle the site. The present study has been facilitated at Hyderabad Municipal Solid Waste Limited, formerly known as Jawahar Nagar dump yard to analyze and assess the feasibility of bio-mining as handling and management alternate to the existing practice of scientific capping. The epicenter of processing and disposal facility is lying approximately on the cross-section of 17°31'24.45"N and 78°35'23.37"E. As per the contract, the comprehensive legacy dumping to be capped in three phases over about 150 acres of area and Ramky has significantly entered the phase two of the operation only within a span of five years by successfully capping more than half of the legacy footprint. Sampling Methodology The waste pile was divided into three layers namely, base, middle, and top. A uniform amount of sample was collected from the successive layers of all five different corners which cover north, south, east, west, and central of the garbage pile. Sampling inspections were performed using a manual auger besides large samples were collected using a JCB excavator. The top six-inch layer of the pile was removed to avoid any contamination while collecting the samples and 5-10 kg of sample was collected from each of the locations. Further, intermediate and bottom layer samples were collected by digging a 500 mm diameter hole through the heap. A composite was prepared by a homogenized blending of all the fifteen grub samples. The blend was distributed into four equal quadrants and the top and bottom quadrants were eliminated diagonally while the left-over quadrants were mixed thoroughly. This process was repeated until a sample of the required bulk of 20 kg is obtained. Surface and subsurface water samples from borewell were collected in and around the facility. Piezometric monitoring borewells located near the landfills were utilized for the subsurface sample collection. While a rainwater pond turned leachate lake named Malkaram was determined as the primary source for leachate collection. Buffer samples were collected from Ambedkar Nagar, the nearby colony exiting at a distance of only 300 meters. Lab-scale Experimentation The representative sample was characterized for composition and further screened through a 70 mm mesh size trommel. The trommel permeate was considered as the organic fraction while the reject was mostly inorganics and inert. The organics were subjected to ASPC. The quantity of the air required is arrived using the method delineated below (Figure 1). MSW Pile size: 2m x 0.5m x 0.5m Volume of pile: 0.5 m3 Average Density of MSW: 620 Kg/m3 Weight of pile: 310 Kg Nitrogen required for matured compost: 9300 mg/kg dry : 9300 X 310 mg : 2.88 x 106 mg : 2.88 Kg Total air required: 2.88 x 100/76 [as Nitrogen in air is 76% by weight] : 3.79 Kg of dry air : 3.79/1.225 m3 [@ 15 deg C density of air 1.225 kg/m3] : 3.1 m3 This air is to be supplied for 100 min / day for 0.5 m pile Air flow rate required: 3.1 x 60/100 = 1.86 m3/h (for practical purpose a flowrate of 2 m3/h was maintained). The maturation period was considered as 28 days and post-maturation, the stabilized material was further cured for 24 hours and screened using 12 mm and 4 mm trommel respectively to obtain the desired product quality and particle size. Whereas, the trommel reject was evenly spreader on the copper trays and dried in an oven at 1050C for 2 hours. The dried material was micronized to the size of 50 mm or below using a scissor and inert such as glass, sand, stone etc. were segregated manually (Mohan and Joseph, 2020). Concurrently, a bench-scale capped landfill prototype was built using the below-mentioned procedure to evaluate the factors such as settlement and slope stability. A 30 mm thick low permeable soil was laid on the top of the waste, followed by a 60 mm layer of compacted clay liner (CCL). Each join between successive liner material was closely monitored. A 1.5 mm thick HDPE liner was placed on the top of the CCL. A 285 GSM geotextile membrane was placed as the successive above layer followed by a 15 mm thick drainage media layer. A further layer of geotextile membrane was placed on top of the drainage media for better stabilization, grip, and strength. The top vegetative soil layer of 45 mm thickness was laid off on top of the geotextile media and St. Augustine grass was rooted (Cortellazzo et al., 2020; Ashford et al., 2000). 2.4 Sample Analysis pH, Electrical Conductivity (EC) and Turbidity of the samples were analyzed using pH, EC-TDS, and Nephelometer of Mettler Toledo. The pH meter was calibrated with the buffer solution of 4.0, 7.0 & 9.12 at a controlled temperature. EC-TDS meter was calibrated with 0.1 M KCL having 12.8 mS/cm of conductivity. Nephelometer was calibrated with Formazine solution of 10 & 100 NTU. Total Dissolved Solids (TDS), (mg/L) was performed using the gravimetric method at 1800C in the oven. Titrimetric parameters such as Total Alkalinity as CaCO3 (mg/L), Total Hardness as CaCO3 (mg/L), Chloride as Cl- (mg/L), Calcium as Ca2+ (mg/L), Residual Free Chlorine (RFC), (mg/L) were analyzed using APHA (American Public Health Associations) method, 23rd Edition, 2017. Total Kjeldahl Nitrogen (mg/L) and Ammonical Nitrogen (mg/L) were performed through distillation followed by titration with H2SO4 as a titrant. Sulphide as S2- was done with the Iodometric method after distillation. Each titrimetric parameter was analyzed in triplicate after standardizing the titrant with required reagents and crossed checked by keeping a check standard. Sodium as Na (mg/L) and Potassium as K (mg/L) were performed using Flame Photometer. The photometer was calibrated with different standards from 10 to 100 (mg/L) standard solutions. The leachate sample was diluted enough to get the value within the standard range and cross-checked with check standards at the same time. Chemical Oxygen Demand (COD), (mg/L) was performed using the open reflux method for 2 hours at 1500C in COD Digestor. Biochemical Oxygen Demand (BOD), (mg/L) was performed using the alkali iodide azide method for 3 days. The samples were kept in a BOD incubator at 270C for 3 days. It was kept in duplicate to have a check on quality control. Sulphate was analyzed by the gravimetric method instead of turbidimetric or through UV-Visible spectrophotometer as its concentration was found more than 40 mg/L. Nitrate as NO3- was analyzed after filtration at 220-275 nm, while Hexavalent Chromium as Cr6+ was analyzed at 540 nm in the UV-Vis. Parameters like Cyanide as CN-, Fluoride as F-, and Phenolic Compounds were gone through a distillation process followed by UV-Vis. The distillation process ensures the removal of interferences presents either positive or negative. For the parameters like Total Iron or Ferric Iron, the samples were digested properly with the required reagents on the hot plate before analyzing in UV-Vis. For the metal analysis the water samples were digested at a temperature of 1000C using aqua regia as a media. The samples were digested to one-fourth of the volume on a hot plate. The recommended wavelengths as per APHA 3120 B were selected for each of the metals. The standard graph was plotted for each of the metals before analysis and crossed checked with the check standard at the same time. Parameters such as bulk density and particle size were performed through the certified beaker and sieve. The percentage of moisture content was estimated using the oven by keeping the compost sample for 2 hours at 1050C. C/N ratio was estimated through CHNS analyzer keeping sulfanilamide as a check standard. The analysis was performed by extracting the desired component in the desired solution prescribed in the method followed by converting the same from mg/L to mg/Kg. RESULTS AND DISCUSSION An exhaustive bench-study has been pursued and real-time samples were collected and analyzed for all possible parameters to determine the pros and cons attributed to both processes. The investigation begins by collecting the samples and concluded by impact assessment studies inclusive of the buffer zone. Both solid, liquid, and gaseous samples were precisely investigated to opt for the best solution. A detailed finding of the investigation is summarized below. Primarily, the representative solid sample was characterized through a manual separation process and the results are portrayed in Figure 1. Compost Characterization ASPC of the organic fraction has resulted in a recovery of 46.7% of the initial load. While 53.3% of the influent mass were inert and barely degradable fraction contributes to reject, the rest 4.1% is miscellaneous process loss. The processed compost was extensively analyzed including for metal contamination and the same is tabulated in Table 1. The value of C/N ratio, OC, TN, K2O, P2O5, and NPK evidently portrays the shortcoming in terms of nutrient availability. Though it is highly enriched in organic carbon and thus the same can be effectively utilized as a soil preconditioner. Ayilara et al. (2020) also reported a similar finding, where the city compost sourced from MSW lagged major plant nutrients. RDF Characterization Processed trommel rejects constitute cloth, rexine, leather, jute, paper, plastics, coir and other inert contributed to RDF. The fraction of inert was as high as 37.2% of the overall RDF mass and it mostly constituted glass and sand. The combined weight of sand and glass fragments contributed 73.5% of the total inert, while the rest was stone and small brickbats. The higher level of silicon associated with the presence of glass and sand yielded siloxane and triggered the possibility of kiln corrosion. A detailed RDF analysis report is enclosed in Table 2. The values explicitly portray the quality of RDF is moderately lower and higher salts concentration is extremely prevalent. With relatively lower NCV and such high salt concentration, the above specimen will certainly pose a corrosion threat to the kiln and shall be either neglected as kiln feed or can be utilized after dilution with Grade III RDF quality. Further, such high ash generation will also induct high transportation and landfill charges. Leachate Characterization The Malkaram leachate lake is the end result of prolonged, slow, and steady mixing of the legacy leachate through the existing fissure cracks in the sheath rock bottom profile. Apparently, the concentration of leachate is significantly lower due to the dilution. Samples were analyzed in triplicates and the mean value is tabulated here in Table 3. The metal concertation and rest of the parameter values are well within the secondary treatment influent range, except for TDS. Thus, a modular aerobic biological treatment unit such as moving bed biofilm bioreactor (MBBR) or membrane bioreactor (MBR) would be a well-suited pick. However, a reverse osmosis (RO) system needs to be installed to get rid of the high TDS content. The permeate of RO can be reused back into the system. Whereas, the reject can be converted into dried powder through forced evaporation mechanisms. The higher concentration of salts in RDF collaterally justifies the elevated TDS level in leachate. In a leachate impact assessment study performed by El-Salam and Abu-Zuid (2015) the reported BOD/COD ratio of 0.69 is greater than double the value of 0.301 reported in Table 3. Though the difference in both the values are quite high, it is relatable and justifiable by the huge age difference of the source waste. The primarily characterized data is of a fresh leachate generated from regular MSW, while the later one is from a decade old waste that barely has any unstabilized organic content. Groundwater Contamination The obvious reason for downward leachate infiltration and osmotic movement facilitates groundwater contamination. Both surface and subsurface water samples were collected within the dump yard and the buffer zone and analyzed using the standard methods. The results are portrayed in Table 4. The slightly alkaline pH of the borewell sample is an indication of the ongoing anaerobic process. The dissolved oxygen value of 3.5 mg/L further validates the correlation. Higher TDS and hardness values are self-indicative of elevated salt concentration in source waste. Eventually, the same interfered with the RDF quality. Positively in the case of all the parameters, a successive decrement in pollution concentration has been spotted from dump ground towards the buffer zone. In a similar study conducted by Singh et al. (2016) at Varanasi, Uttar Pradesh the reported concentration of the parameters is significantly higher than reported in Table 4. The basic reason behind variation is the dissimilarities of the local soil profile. The sandy and clay loam soil profile of Varanasi allows a greater rate of percolation and infiltration. While the bottom sheath rock profile at Jawahar Nagar permits the only a minute to little percolation rate. The difference in percolation rate is directly correlated to the concentration levels in this case. Contrarily, Kurakalva et al. (2016) have reported much-elevated pollutant concertation both in ground and surface water for a study conducted at the same site in 2016. The higher concentration is relatable to the fact of the non-closure of the open dump back then. Capping activity had at Jawahar Nagar gained its pace 2018 onwards and capping for the primary section of 70 acres got concluded only during mid of 2019. Due to the decrement in runoff and percolation, the quality of both surface and subsurface water has improved drastically. Impact Assessment The odor and groundwater contamination are two of the primary issues that triggered a massive public agitation initially. The root causes of both the issues are identified as rainwater percolation and anaerobic digestion respectively. Eventually, the completion of the capping process would resolve both the problems effectively. Other non-tangential impacts include nausea; headache; irritation of the eye, nasal cavity, and throat; diarrhoeal diseases; vector-borne disease, cattle toxicity etc. Scientific capping can easily cater as the wholesome solution for all (Cortellazzo et al., 2020). Yu et al. (2018) had performed an extensive study to comprehend the relativity of respiratory sickness and MSW borne air pollution. The study made a couple of dreadful revelations such as gases released due to the anaerobic digestion of MSW such as methane, hydrogen sulphide, and ammonia incur detrimental impact on Lysozyme and secretory immunoglobulin A (SIgA). While SO2 was reported as the lung capacity and functionality reducer. Further, a gender-specific study executed by the same research group revealed, air pollution impacts more severely on male children than the female and retards immune functions. Presently, the area of 351 acres has been developed as Asia’s one of the largest state of the art municipal solid waste processing and disposal facility by Ramky Enviro Engineers Limited. This ensured zero dumping and no further environmental interventions. As legal compliance, the facility monitors the quality of groundwater and ambient air quality in and around the facility on monthly basis to assure the biosafety. The variation in concentration of various monitoring parameters between 2012 to 2020 is summarized in Figure 2. The concentration of each of the parameters are showcased in ppm and a standard equipment error was settled at 3% for respirable dust sampler and multi-gas analyzer (Taheri et al., 2014). Despite all parameter values have gradually increased except for methane, the facility still managed to maintain them well under the regulatory limits. The decrement in methane concentration is directly correlated to the practice of aerobic composting and aeration-based secondary treatment that prevented the formation of the anaerobic atmosphere and henceforth methane generation. While for the rest of the parameters the increment in values is quite substantial and predictable due to the sudden escalation in MSW generation in the past decade in correlation with Gross domestic product (GDP) enhancement. The observed and interpreted impacts due to the elevated pollutant level are in-line with the georeferenced findings reported by Deshmukh and Aher (2016) based on a study conducted at Sangamner, Maharashtra. CONCLUSION The study critically analyzed and investigated every techno-environmental and socio-economic aspect correlated to open dumping. The bench-scale experimentation revealed the efficiency of the single liner scientific capping is fair enough to eliminate any further rainwater infiltration, however, it has no control over the generation of leachate due to the inherent moisture. Internal moisture related issue was anyhow compensated with pertinent compaction prior to dispose of the waste. Contrarily, both the products derived through the biomining process namely, compost and RDF lagged quality due to scantier nutrient content and higher salt and silicon content respectively. Besides, impact assessment studies concede the pollutant concentration in groundwater in and around the plant has drastically diminished post-July 2019 due to the partial completion of waste capping. It also abetted lowering the dust and odor issues relatively in the surrounding. ACKNOWLEDGMENT The authors would like to sincerely acknowledge GHMC, Hyderabad Integrated Municipal Solid Waste Limited, and Ramky Enviro Engineers Limited for enabling us to pursue the sample collection and other necessary onsite activities. Further, the authors would like to register profound acknowledgment to EPTRI for supporting us with the essential experimental facilities. REFERENCES Sharma, A., Gupta, A.K., Ganguly, R. (2018), Impact of open dumping of municipal solid waste on soil properties in mountainous region. 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AbstractWith the transfer-matrix method, conditions of extraordinary electromagnetic resonant tunneling through all combinations of three-layer nonmagnetic (µr = 1) media containing epsilon-negative (ENG) and doublepositive (DPS) slabs were explored. We show that abnormal phenomena can occur in the ENG-DPS-ENG structure, without any restriction on the permittivity of the DPS layer. Changes of transmittance as a function of frequency and incidence angle for a dispersive, lossy model are also calculated, and the results demonstrate the possibility of exhibition this counterintuitive tunneling phenomenon in the DPS-ENG-DPS structure within a wide range of incidence angles.

AbstractA couple ring enclosed circular geometric resonator (CRECGR) based dual-band polarization insensitive metamaterial (MM) with high effective medium ratio (EMR), and excellent absorptance is proposed in this study, which can be utilized as a sensor and absorber in the 5G sub-6 GHz frequency range. A circular geometry-based unique patch has been introduced in the proposed unit cell to achieve high polarization insensitive properties with excellent absorption for the 5G sub-6 GHz spectrum. The distinctive feature of this proposed CRECGR unit cell is its simple and unique structure with a high EMR of 11.13, polarization insensitive up to 180°, and epsilon negative (ENG) properties, including a negative refractive index and near-zero permeability for 5G sub-6 GHz applications. Furthermore, this designed unit cell yields excellent absorption properties with high quality factor. The designed MM unit cell is fabricated on low loss Rogers RT5880 printed media with an electrical dimension of 0.089λ × 0.089λ × 0.017λ. The performance of the designed CRECGR metamaterial is determined using Computer Simulation Technology (CST), Advanced Design Software (ADS), and measurements. The CRECGR unit cell offers dual resonances at 3.37 GHz and 5.8 GHz, covering the 5G sub-6 GHz band with ENG, near-zero permeability and negative index. The polarization insensitive properties of the unit cell were also investigated for maximum angle of incidence, which confirmed the identical response. The simulated outcome is verified by experiment with excellent accordance. Moreover, the unit cell performance with a complete backplane is explored, noting a maximum absorption of 99.9% for all normal and oblique incidence waves, suitable for sensing and antenna systems. In addition, the suggested unit cell sensing performance is evaluated using the permittivity-based sensing model. The proposed MM outperforms recent related studies in terms of polarization insensitivity up to 180°, high insensitive absorptivity, high EMR, and sensing applications. These features prove that the proposed CRECGR metamaterial is perfect for 5G Applications.

Abstract In this paper, a spatially selective (SS) structure is investigated through the critical jump characteristic when the permittivity of the epsilon-negative (ENG) materials approaches zero. In the range where the incident frequency of the TE wave is 5.1-5.6 c/d and that of the TM wave is 5.0-5.7 c/d, the transmittance exceeds 0.85 and the squareness factor (SF) surpasses 0.9. By adjusting the electronic plasma frequency to affect the position of epsilon-near-zero (ENZ) frequency, a wide-angle control of the SS characteristic extent is produced. Using the fragility of the critical angle to the background analyte medium, a variable refractive index sensor is proposed. When the electronic plasma frequency is continuously altered, the analyte with the measurement range of 1.1-1.3, 1.3-1.5, 1.5-1.7, or 1.7-1.9 can be detected. The corresponding sensitivity is 57.1 degrees/RIU, 48.8 degrees/RIU, 40.0 degrees/RIU, or 32.8 degrees/RIU. The elongation in the thickness of ENG materials contributes to the improvement of the SF, executing the discrimination of Vibrio cholera, E. coli, and Shigella flexneri. The effects of losses on device performance are also briefly considered. Different from previous studies, the proposed SS selective structure adopts a new ENZ mechanism, and can simplify the design of the structure to a certain extent, and has a possibility in the design of highly sensitive sensors.

AbstractThis study has investigated the impact of inverse G-like shape resonators that exhibited epsilon negative (ENG) and near-zero refractive index (NZI) properties for multi-band wireless communications applications. The electrical measurement of structure is 0.118λ × 0.118λ × 0.021 λ, which is calculated at 3.94 GHz. FR-4 is used as a substrate layer, and the resonator is designed on it. This structure is manifested in the ENG and NZI characteristics within the frequency range of 3.8–4.17, 7.68–8.54, 10.67–11.36 GHz, and 4.07–4.15 and 8.29–8.37 GHz, respectively. This study also manifests the polarization insensitivity nature of 0°–90°, and the incident angle is investigated up to 60° for both TE and TM modes. The proposed structure achieves triple resonance at 3.94 GHz, 8.08 GHz, and 11.17 GHz, respectively, included in the S, C, and X frequency bands. The CST Microwave Studio 2019 software is conducted to design, develop, perform analysis, investigate electromagnetic properties, and extract effective medium parameters. The Advanced Design Software (ADS) is used to model the equivalent circuit of the unit cell. The simulated, measured, and ADS results verified the scattering parameter performance. The EMR value of the structure is 8.47, indicating the structure's compactness. The compact design with simplicity, ENG, and NZI properties make the proposed structure significant for microwave application, mainly to enhance the antenna bandwidth and gain filter design. ENG and NZI properties the operation frequency stability and efficiency for low earth orbit nanosatellite communications.

AbstractIn this paper, a tuned metamaterial (MTM) consisting of a symmetric split ring resonator is presented that exhibits epsilon negative (ENG), near zero permeability and refractive index properties for multiband microwave applications. The proposed metamaterial is constituted on a Rogers (RT-5880) substrate with 1.57 mm thickness and the electrical dimension of 0.14λ × 0.14λ, where wavelength, λ is calculated at 4.2 GHz. The symmetric resonating patch is subdivided into four equal and similar quartiles with two interconnecting split rings in each quartile. The quartiles are connected at the center of the substrate with a square metal strip with which four tuning metal strips are attached. These tuning metal strips are acted as spacers between four quartiles of the resonator patch. Numerical simulation of the proposed design is executed in CST microwave studio. The proposed MTM provides four resonances of transmission coefficient (S21) at 4.20 GHz, 10.14 GHz, 13.15 GHz, and 17.1 GHz covering C, X and Ku bands with negative permittivity, near zero permeability and refractive index. The calculated effective medium ratio (EMR) is 7.14 at 4.2 GHz indicates its compactness. The resonance frequencies are selective in nature which can be easily tuned by varying the length of the tuning metal stubs. The equivalent circuit of the proposed MTM is modelled in Advanced Design Software (ADS) that exhibits a similar S21 compared with CST simulation. Surface current, electric and magnetic fields are analyzed to explain the frequency tuning property and other performances of the MTM. Compact size, ENG with near zero permeability and refractive index along with frequency selectivity through tuning provides flexibility for frequency selective applications of this MTM in wireless communications.

Social media websites, such as Facebook and Twitter, are starting to become places where people present and evaluate various events in the world such as terrorist attacks in London, Barcelona, Berlin, Brussels in 2017. Moreover, these websites influence value perception of their users and readers. These new technologies allow people to exchange views at the very moment of the event and time zone and location users of the platforms do not have a significant role in this exchange process. However, this capability of these new technologies can have a negative impact on some social groups. The aim of this article is to provide an analysis of the discourse in social media on refugees’ integration in their respective host societies by analysing the content in Lithuanian language Facebook pages ‘Priimsiu pabėgėlį’ (eng. ‘Refugees Welcome’) and ‘Visuomeninis komitetas prieš priverstinę imigraciją’ (eng. ‘Public Committee against Forced Immigration’).