Relevant bibliographies by topics / Epitope library

Academic literature on the topic 'Epitope library'

Author: Grafiati
Published: 11 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Epitope library.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Epitope library"

1

Moriki, Takanori, Ichiro N. Maruyama, Yusuke Yamaguchi, Atsuko Igari, Yasuo Ikeda, and Mitsuru Murata. "Identification of ADAMTS13 Epitopes Required for Binding to von Willebrand Factor Using Lambda Phage Surface Display." Blood 110, no. 11 (November 16, 2007): 2707. http://dx.doi.org/10.1182/blood.v110.11.2707.2707.

Full text
Abstract:
Abstract The metalloprotease ADAMTS13 cleaves multimeric von Willebrand factor (VWF) to regulate VWF-mediated thrombus formation. We planned to search core epitopes of ADAMTS13 that is required for its binding to VWF. We constructed a random cDNA fragment library expressing various peptides of ADAMTS13 on the surface of lambda phage and screened the library using immobilized VWF as a probe. After the first screening, the C-terminus of the spacer domain from Arg670 to Glu684 (termed as epitope-1) and the middle of the cysteine-rich domain from Arg484 to Arg507 (epitope-2) were determined as epitopes. When we added the synthetic epitope-1 peptide to the second screening, a new site, from Pro618 to Glu641 (epitope-3), was found in the middle of spacer domain. While the presence of synthetic epitope-2 peptide did not affect the subsequent screening, the presence of epitope-3 peptide enhanced the isolation of clones encoding epitope-1. These results suggest that ADAMTS13 epitopes-1, -2 and -3 may interact with each other for their binding to VWF. From screening in the presence of any combination or all of the three synthetic peptides, however, no new VWF binding site was uncovered. To examine the effect of divalent metal cations on the binding of ADAMTS13 epitopes to immobilized VWF, screening was carried out in the presence or absence of 5 mM of EDTA. No new epitope site was found. We next explored inhibitory effect of the synthetic epitope peptides on ADAMTS13 protease activity using recombinant ADAMTS13 and FRETS-VWF73 as a substrate. Synthetic epitopes-2 and -3 peptides markedly inhibited the cleavage of VWF by ADAMTS13, while the synthetic epitope-1 peptide did not as efficiently as epitopes-2 and -3. The stronger inhibitory effect of epitope-3 peptide than that of epitope-1 peptide was confirmed by SDS-agarose gel electrophoresis analysis of cleavage products of denatured multimeric VWF molecules by recombinant ADAMTS13. This was consistent with the dissociation constants for the three synthetic peptides with immobilized VWF determined by surface plasmon resonance, in which epitopes-2 and -3 have higher affinities for VWF than that of epitope-1. The results described above suggest that ADAMTS13 may initially bind to immobilized VWF through the sites of epitope-1 and epitope-2 with relatively weak affinity. The binding of epitope-1 to VWF may subsequently induce the conformational change of VWF, thereby exposing a binding site for epitope-3 for the efficient catalytic cleavage of VWF by ADAMTS13.
APA, Harvard, Vancouver, ISO, and other styles
2

Ostrowski, M., J. A. Galeota, A. M. Jar, K. B. Platt, F. A. Osorio, and O. J. Lopez. "Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 Ectodomain." Journal of Virology 76, no. 9 (May 1, 2002): 4241–50. http://dx.doi.org/10.1128/jvi.76.9.4241-4250.2002.

Full text
Abstract:
ABSTRACT After infection of swine with porcine reproductive and respiratory syndrome virus (PRRSV), there is a rapid rise of PRRSV-specific nonneutralizing antibodies (NNA), while neutralizing antibodies (NA) are detectable not sooner than 3 weeks later. To characterize neutralizing epitopes, we selected phages from a 12-mer phage display library using anti-PRRSV neutralizing monoclonal antibody (MAb) ISU25-C1. In addition, phages carrying peptides recognized by swine antibodies with high seroneutralizing titer were isolated after subtracting from the library those clones binding to swine anti-PRRSV serum with no neutralizing activity. Two epitopes located in the ectodomain of PRRSV GP5 were identified. One of these epitopes, which we named epitope B, was recognized both by neutralizing MAb ISU25-C1 and swine neutralizing serum (NS) but not by swine nonneutralizing serum (NNS), indicating that it is a neutralizing epitope. Epitope B is sequential, conserved among isolates, and not immunodominant. Antibodies directed against it are detected in serum late after infection. In contrast, the other epitope, which we named epitope A, is hypervariable and immunodominant. Antibodies against it appear early after infection with PRRSV. This epitope is recognized by swine NNA but is not recognized by either neutralizing MAb ISU25-C1 or swine NA, indicating that it is not involved in PRRSV neutralization. During infection with PRRSV, epitope A may act as a decoy, eliciting most of the antibodies directed to GP5 and delaying the induction of NA against epitope B for at least 3 weeks. These results are relevant to the design of vaccines against PRRSV.
APA, Harvard, Vancouver, ISO, and other styles
3

Buchli, Rico, Rodney S. VanGundy, Sonja Plompen, Aaron D. Rennels, Nicholas J. Ede, and William H. Hildebrand. "Mining for Treasures: Systematic Profiling of 8-, 9-, 10-, and 11-mer HLA-B*0702-restricted CD8+ T cell epitopes from the Influenza A/Puerto Rico/8/34 (H1N1) Virus Hemagglutinin with Potential Application in Vaccine Development (B193)." Journal of Immunology 178, no. 1_Supplement (April 1, 2007): LB40. http://dx.doi.org/10.4049/jimmunol.178.supp.b193.

Full text
Abstract:
Abstract Identification of T cell epitopes is of crucial importance for the development of cancer and viral T cell eliciting vaccines. Here, we define CD8+ T cell epitopes using a novel high-throughput system for the synthesis and screening of peptide libraries that include all dominant epitope lengths (8–11mers) for MHC class I molecules. These representative peptide libraries are tested for their ability to bind various MHC class I molecules in a competitive manner. This comprehensive screening strategy begins with the synthesis of an overlapping truncated peptide library containing mixes of four peptides with a common C terminus, but having a length of 8, 9, 10, or 11 residues. Following the initial screen, positive hits are resolved into individual epitope responses. In order to identify HLA-B*0702-restricted epitopes, a peptide library was prepared for the influenza A virus Hemagglutinin (HA) protein. Screening results revealed 63 high/medium affinity epitopes at various sizes. A number of these putative influenza epitopes coincide with directly discovered influenza epitopes as well as influenza epitopes reportedly recognized by CTL. In conclusion, this study demonstrates that the rapid and methodical screening of T cell immune epitope libraries can help in detecting and monitoring infections as well as enabling immune intervention strategies with respect to treatment or prevention of the disease.
APA, Harvard, Vancouver, ISO, and other styles
4

Jette, D. C., F. T. Kreutz, B. A. Malcolm, D. S. Wishart, A. A. Noujaim, and M. R. Suresh. "Epitope mapping of prostate-specific antigen with monoclonal antibodies." Clinical Chemistry 42, no. 12 (December 1, 1996): 1961–69. http://dx.doi.org/10.1093/clinchem/42.12.1961.

Full text
Abstract:
Abstract Prostate-specific antigen (PSA) is a widely used marker for screening and monitoring prostate cancer. We identified and characterized the epitopes of two anti-PSA monoclonal antibodies (mAbs) designated B80 and B87. The epitopes were initially mapped as nonoverlapping by developing a sandwich immunoassay to measure PSA with the two anti-PSA mAbs. The two antibodies do not cross-react with homologous pancreatic kallikrein, but recognize epitopes unique to PSA. B80 and B87 can recognize both free and complexed PSA and hence measure total PSA. Epitope scanning and bacteriophage peptide library affinity selection procedures were used to identify and locate an epitope on PSA. A possible epitope for B80 was identified as being located on or near PSA amino acid residues 50-58 (-GRH-SLFHP-). The epitope for B87 was likely on an exposed nonlinear conformational determinant, unique to PSA, and not masked by the binding of B80 or alpha 1-antichymotrypsin.
APA, Harvard, Vancouver, ISO, and other styles
5

Chen, Longxin, Chaoyang Zhu, Hui Guo, Runting Li, Limeng Zhang, Zhenzhen Xing, Yue Song, et al. "Epitope-directed antibody selection by site-specific photocrosslinking." Science Advances 6, no. 14 (April 2020): eaaz7825. http://dx.doi.org/10.1126/sciadv.aaz7825.

Full text
Abstract:
Currently, there are no methods available offering solutions to select and identify antibodies binding to a specific conformational epitope of an antigen. Here, we developed a method to allow epitope-directed antibody selection from a phage display library by photocrosslinking bound antibodies to a site that specifically incorporates a noncanonical amino acid, p-benzoyl-l-phenylalanine (pBpa), on the target antigen epitope. By one or two rounds of panning against antibody phage display libraries, those hits that covalently bind to the proximity site of pBpa on specific epitopes of target antigens after ultraviolet irradiation are enriched and selected. This method was applied to specific epitopes on human interleukin-1β and complement 5a. In both cases, more than one-third of hits identified bind to the target epitopes, demonstrating the feasibility and versatility of this method.
APA, Harvard, Vancouver, ISO, and other styles
6

Suprun, Maria, Scott H. Sicherer, Robert A. Wood, Stacie M. Jones, Donald Y. M. Leung, A. Wesley Burks, David Dunkin, et al. "Mapping Sequential IgE-Binding Epitopes on Major and Minor Egg Allergens." International Archives of Allergy and Immunology 183, no. 3 (November 24, 2021): 249–61. http://dx.doi.org/10.1159/000519618.

Full text
Abstract:
<b><i>Introduction:</i></b> Molecular studies of hen’s egg allergens help define allergic phenotypes, with IgE to sequential (linear) epitopes on the ovomucoid (OVM) protein associated with a persistent disease. Epitope profiles of other egg allergens are largely unknown. The objective of this study was to construct an epitope library spanning across 7 allergens and further evaluate sequential epitope-specific (<i>ses-</i>)IgE and <i>ses-</i>IgG<sub>4</sub> among baked-egg reactive or tolerant children. <b><i>Methods:</i></b> A Bead-Based Epitope Assay was used to identify informative IgE epitopes from 15-mer overlapping peptides covering the entire OVM and ovalbumin (OVA) proteins in 38 egg allergic children. An amalgamation of 12 B-cell epitope prediction tools was developed using experimentally identified epitopes. This ensemble was used to predict epitopes from ovotransferrin, lysozyme, serum albumin, vitellogenin-II fragment, and vitellogenin-1 precursor. <i>Ses-</i>IgE and <i>ses-</i>IgG<sub>4</sub> repertoires of 135 egg allergic children (82 reactive to baked-egg, the remaining 52 tolerant), 46 atopic controls, and 11 healthy subjects were compared. <b><i>Results:</i></b> 183 peptides from OVM and OVA were screened and used to create an aggregate algorithm, improving predictions of 12 individual tools. A final library of 65 sequential epitopes from 7 proteins was constructed. Egg allergic children had higher <i>ses-</i>IgE and lower <i>ses-</i>IgG<sub>4</sub> to predominantly OVM epitopes than both atopic and healthy controls. Baked-egg reactive children had similar <i>ses-</i>IgG<sub>4</sub> but greater <i>ses-</i>IgE than tolerant group. A combination of OVA-sIgE with <i>ses-</i>IgEs to OVM-023 and OVA-028 was the best predictor of reactive phenotype. <b><i>Conclusion:</i></b> We have created a comprehensive epitope library and showed that <i>ses-</i>IgE is a potential biomarker of baked-egg reactivity.
APA, Harvard, Vancouver, ISO, and other styles
7

Holzem, Achim, Jörg M. Nähring, and Rainer Fischer. "Rapid identification of a tobacco mosaic virus epitope by using a coat protein gene-fragment–pVIII fusion library." Journal of General Virology 82, no. 1 (January 1, 2001): 9–15. http://dx.doi.org/10.1099/0022-1317-82-1-9.

Full text
Abstract:
This study describes the identification of the epitope recognized by the tobacco mosaic virus (TMV) coat protein (CP)-specific monoclonal antibody 29 (MAb29) by displaying a CP gene-fragment library on pVIII of filamentous phage M13. More than 80% of the clones isolated after one round of panning bound specifically to MAb29. DNA sequencing of ten randomly chosen MAb29-specific clones and subsequent sequence comparison revealed a common seven amino acid epitope (ELIRGTG) representing amino acids 131–137 of the TMV CP. The reactivity of MAb29 in competition ELISA towards glutathione S-transferase fused to this epitope was stronger than that towards full-length wild-type TMV CP, confirming the epitope sequence determined by gene-fragment phage display. This demonstrated that gene-fragment libraries displayed on the phage surface as fusion proteins with the filamentous bacteriophage gene VIII are useful tools for rapid identification of linear epitopes recognized by MAbs.
APA, Harvard, Vancouver, ISO, and other styles
8

Yuan, Tom Z., Ana G. Lujan Hernandez, Erica Keane, Qiang Liu, Fumiko Axelrod, Shweta Kailasan, Madeleine Noonan-Shueh, Mohammad Javad Aman, Aaron K. Sato, and Yasmina N. Abdiche. "Rapid exploration of the epitope coverage produced by an Ebola survivor to guide the discovery of therapeutic antibody cocktails." Antibody Therapeutics 3, no. 3 (July 2020): 167–78. http://dx.doi.org/10.1093/abt/tbaa016.

Full text
Abstract:
ABSTRACT Background Development of successful neutralizing antibodies is dependent upon broad epitope coverage to increase the likelihood of achieving therapeutic function. Recent advances in synthetic biology have allowed us to conduct an epitope binning study on a large panel of antibodies identified to bind to Ebola virus glycoprotein with only published sequences. Methods and Results A rapid, first-pass epitope binning experiment revealed seven distinct epitope families that overlapped with known structural epitopes from the literature. A focused set of antibodies was selected from representative clones per bin to guide a second-pass binning that revealed previously unassigned epitopes, confirmed epitopes known to be associated with neutralizing antibodies, and demonstrated asymmetric blocking of EBOV GP from allosteric effectors reported from literature. Conclusions Critically, this workflow allows us to probe the epitope landscape of EBOV GP without any prior structural knowledge of the antigen or structural benchmark clones. Incorporating epitope binning on hundreds of antibodies during early stage antibody characterization ensures access to a library’s full epitope coverage, aids in the identification of high quality reagents within the library that recapitulate this diversity for use in other studies, and ultimately enables the rational development of therapeutic cocktails that take advantage of multiple mechanisms of action such as cooperative synergistic effects to enhance neutralization function and minimize the risk of mutagenic escape. The use of high-throughput epitope binning during new outbreaks such as the current COVID-19 pandemic is particularly useful in accelerating timelines due to the large amount of information gained in a single experiment.
APA, Harvard, Vancouver, ISO, and other styles
9

Maier, Richard H., Christina J. Maier, Raphaela Rid, Helmut Hintner, Johann W. Bauer, and Kamil Önder. "Epitope Mapping of Antibodies Using a Cell Array–Based Polypeptide Library." Journal of Biomolecular Screening 15, no. 4 (March 16, 2010): 418–26. http://dx.doi.org/10.1177/1087057110363821.

Full text
Abstract:
The authors describe a technique for mapping the epitopes of protein antigens recognized by mono- or polyclonal antibodies. This method is based on a recombinant polypeptide library, expressed in a bacterial expression system, arrayed at high density, and tested on a membrane with automated procedures. The authors analyzed the epitope of a commercially available monoclonal antibody to vitamin D receptor (VDR). About 2300 overlapping VDR peptides were screened on a test array, and a contiguous stretch of 37 amino acids was identified as the epitope. Its authenticity was confirmed by Western blotting and an immunofluorescence competition assay on human skin tissue samples. The authors define the proposed method as a cell-based protein or peptide array that is adaptable to many applications, including epitope mapping of antibodies and autoantibodies, autoantigen detection from patient sera, whole-proteome approaches such as protein-peptide interactions, or selection of monoclonal antibodies from polyclonal sera. The advantages of this method are (a) its ease of protein array production based on well-established bacterial protein/peptide expression procedures; (b) the large number of printable colonies (as many as ~25,000) that can be arrayed per membrane; (c) there is no need for protein purification of recombinantly expressed proteins; (d) DNA, rather than protein, is the starting material to generate the arrays; and (e) its high-throughput and automatable format.
APA, Harvard, Vancouver, ISO, and other styles
10

Tsai, D. E., and J. D. Keene. "In vitro selection of RNA epitopes using autoimmune patient serum." Journal of Immunology 150, no. 3 (February 1, 1993): 1137–45. http://dx.doi.org/10.4049/jimmunol.150.3.1137.

Full text
Abstract:
Abstract Nucleotide-specific autoimmune epitopes have not been precisely defined despite the fact that certain kinds of DNA and RNA species are known to bind autoantibodies. Our laboratory has used nucleic acid epitope libraries, consisting of randomized RNA pools, to select specific RNA conformers recognized by antibodies, including a peptide-specific antibody. In the present study, serum from a patient with systemic lupus erythematosus was used to select ligands from an RNA epitope library. The selected RNA contained sequences that were found to be similar to regions within the U1 small nuclear RNA, previously shown to react with autoantibodies. Furthermore, the selected RNA epitopes were able to inhibit autoantibody reactivity with specific regions of U1 RNA, thus demonstrating their immunologic cross-reactivity with the natural RNA epitope. Although the origins of nucleic acid-binding autoantibodies are not understood, the identification of these defined U1 RNA epitopes, in regions of the RNA where cell proteins are not known to bind, is most compatible with models of immunologic cross-reactivity or with direct presentation to the immune system rather than with anti-Id models. These experiments demonstrate that RNA epitope libraries may be used to reveal the fine specificity of autoimmune recognition and provide a useful approach to study RNA-protein interactions.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Epitope library"

1

Cortini, Andrea. "Serum profiling and autoantibodies identification in Multiple Sclerosis using epitope and CSF IgG phage display libraries." Doctoral thesis, Università degli studi di Trieste, 2009. http://hdl.handle.net/10077/3073.

Full text
Abstract:
2007/2008
Multiple sclerosis (MS) is considered the prototype of inflammatory autoimmune diseases of the central nervous system (CNS). The typical feature of the disease is the plaques of demyelination. The evolution of the plaque lesion in MS implicates an inflammatory phase followed by a recovery of functional myelin; a second step is the chronic progressive disease with axonal loss. The earlier phase of MS may be mediated by an autoimmune reaction. Whereas the role of T cells in MS pathogenesis is well established, the role of B cells and autoantibodies in demyelination and plaque formation is still unresolved. However several evidences suggest a contribute of autoantibodies in MS pathogenesis. B cells and myelin specific autoantibodies are present in the sclerosis plaques, and there is an increased production of immunoglobulin (Ig) in the cerebrospinal fluid (CSF) of more than 90% of MS patients . Typically these Ig present an oligoclonal pattern and sequencing of oligoclonal IgG showed extensive somatic mutations suggesting B cell clonal expansion and a specific antigen-driven immune response. The most extensively studied putative autoantigens are components of CNS myelin (myelin basic protein MBP, proteolipid protein PLP, myelin oligodendrocyte glycoprotein MOG). The autoantibodies in MS recognize both linear and conformational epitopes, but at present the conformational epitopes of myelin proteins have not been identified. For example, in MS, the T-cell receptors of autoreactive T lymphocytes recognize various peptides of the MBP, and, in EAE, the anti-MOG antibodies recognize only conformational epitopes. Furthermore, the progression of MS is accompanied by the decline of primary T-cell autoreactivity and by the concurrent emergence of neo-autoreactivity (epitope spreading). However recent investigation have showed that no myelin antigens, like neuron-specific enolase (NSE), retinal arrestin, beta-arrestin, may also have a role in MS pathogenesis. Autoimmunity against these antigens may be linked to neurodegeneration, defective remyelination, and predisposition to uveitis in multiple sclerosis. Several strategies, involving the phage display technology, have been employed in the attempt to discover the antigen that drives the immune response in MS. A first strategy depends on the cloning of IgG repertoire of MS patients in a phage display library screened with brain sections or known antigens. Another strategy involves large phage display libraries of random peptides screened with IgG of CSF in order to identify peptides recognized by antibodies present in CSF of MS patients. Phage display is a technique which involves the coupling of phenotype to genotype in a selectable format. It has been extensively used in molecular biology to study protein-protein interactions and to select antibodies against a wide range of different antigens. In this project we have proposed: 1. to study the autoimmune response in MS by using the phage display for the expression of antibodies involved in the disease. We wanted to make a ScFv library from B cells of CSF of different multiple sclerosis patients, to employ as tool to select a phage display Human Brain cDNA library for the identification of new antigens recognized by the immune sistem in MS patients. 2. To produce single gene mini library of putative antigens (MBP,PLP, MOG) for the generation of epitope chips to use for serotyping the immune response in different patients 3. To investigate the feasibility to use a single gene phage display mini-library as tool for epitope mapping (both linear and conformational) of novels autoantigens 4. To investigate the role of NSE(neuron specific enolase), a new possible no myelin autoantigen in multiple sclerosis, in the pathogenesis of the disease and the usefulness as possible diagnostic marker. Results: Scope 1 B-cells from liquor of two MS patients were centrifuged and the total RNA was extracted from the pellets. Total RNA was retrotranscripted and variable region of heavy and light chain of the antibodies were amplified by PCR. Heavy chain and light chain were assorted and assembled before to be cloned in the phagemid vector pDAN 5. A 2x104 independent clones library was obtained and analyzed by PCR and fingerprinting. A diversity of 30,8% for heavy chain and 72,7% for light chain was established. ScFv library was used to select a phage display Human Brain cDNA library. 17 clones with an high reactivity were obtained and after sequencing 6 clones on 17 have shown to be the same antigen(antigen A ); the reactivity on other two antigens obtained with the selection (antigen B and C) of CSF from 18 MS patients and 16 patients with other neurological disease (OND) was tested by ELISA to evaluate diagnostic value of this protein. The results shown that SM response was statistically different from OND response; the ELISA test gave a specificity of 94,12% and a significance of 53,85 %. The reactivity for the antigen B was also evaluated on sera of MS patients and controls. The MS response was statistically different from OND response and shown a specificity of 97,44% and a significance of 58,62 %. Scope 2&3 We have generated three single gene mini libraries of the major antigens in MS (MBP, MOG and PLP); cDNA of each gene was obtained by RT-PCR and after fragmentation cloned in a phagemid vector (pEP1) to obtain a mini-library for each gene. We have obtained a 2x105 for MBP, 2.4x104 for MOG and 1.6x106 for PLP independent clones library. MBP and MOG libraries were characterized by PCR and fingerprinting. Sequencing analysis shown that the entire MBP transcript variant 7 mRNA (664-1177 nt) and MOG isoform alpha 1 mRNA (262-918 nt) were represented in the respective library. To testing the capacity of selecting a single epitope from our libraries, we have performed a selection test with a commercial monoclonal antibody that recognize MBP 82-98 epitope; after three selection panning all selected clones contain the nucleotidic sequence 906- 956 nt (MBP transcript variant 7 mRNA) which encodes the immunogenic epitope recognized by the monoclonal antibody. Scope 4 The reactivity of sera from 31 MS patients and 14 healthy controls was tested by ELISA on NSE ; statistical analysis of the results shown that the two populations were significantly different.
XXI Ciclo
1981
APA, Harvard, Vancouver, ISO, and other styles
2

Marson, Lorena. "Phage-display epitope library development for biomarkers identification in autoimmune diseases of the Central Nervous System." Doctoral thesis, Università degli studi di Trieste, 2012. http://hdl.handle.net/10077/7405.

Full text
Abstract:
2010/2011
The principal aim of my PhD was the setting of a protocol for the creation of phage libraries to display cDNA fragments encoding real ORF sequences, that could correspond to potential epitopes. A similar phage display library contains all the potential ORF repertoire of a cell or tissue. This tool can be specially used in the study of autoimmune diseases to perform different kind of analysis, such as the identification of epitopes involved in pathological reaction, the comparison between healthy and pathological conditions, or between different pathological conditions. A complex protocol was developed. It provides for: cDNA normalization, cDNA fragmentation to obtain peptides with useful size, and ORF enrichment to obtain really coding fragments. With this system we have created a epitopes library from Human brain mRNA.
Il principale obiettivo del mio lavoro di ricerca è la messa a punto di un protocollo per la costruzione di librerie fagiche di frammenti di cDNA codificanti per frammenti ORF, e che quindi potrebbero corrispondere a potenziali epitopi. Questo tipo di librerie contengono, potenzialmente, tutto il repertorio ORF di una cellula o di un tessuto e possono quindi essere utilizzate nello studio di malattie autoimmuni al fine di identificare nuovi epitopi coinvolti nella risposta immunitaria, di fare un confronto tra lo stato patologico e quello sano o tra diverse condizioni patologiche. Abbiamo quindi messo a punto un complesso protocollo che prevede: la normalizzazione del cDNA, la sua frammentazione per ottenere peptidi di dimensioni opportune, e l'arricchimento in frammenti realmente codificanti. Con questo sistema abbiamo realizzato una libreria di epitopi a partire da mRNA di cervello umano.
XXIV Ciclo
1984
APA, Harvard, Vancouver, ISO, and other styles
3

Löfblom, John. "Staphylococcal surface display for protein engineering and characterization." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

Full text
Abstract:
Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. Directed evolution has provided researchers with a tool for circumventing our limited knowledge and hence the possibility to create novel molecules that by no means could have been designed today. The approach is based on construction of high-complexity combinatorial libraries from which protein variants with desired properties can be selected. Engineered proteins are already indispensable tools in nearly all areas of life science and the recent advent of mainly monoclonal antibodies as therapeutic agents has directed even more attention to the field of combinatorial protein engineering. In this thesis, I present the underlying research efforts of six original papers. The overall objective of the studies has been to develop and investigate a new staphylococcal surface display method for protein engineering and protein characterization. The technology is based on display of recombinant proteins on surface of the Gram-positive bacteria Staphylococcus carnosus. In two initial studies, two key issues were addressed in order to improve the protein engineering method in regard to affinity discrimination ability and transformation efficiency. The successful results enabled investigation of the staphylococcal display system for de novo generation of affibody molecules from large combinatorial libraries. In this study, a high-complexity protein library was for the first time displayed on surface of Gram-positive bacteria and by means of fluorescence-activated cell sorting, specific affinity proteins for tumor necrosis factor-alpha were isolated. Moreover, in following papers, the staphylococcal display method was further improved and investigated for affinity determination, soluble protein production and epitope mapping purposes in order to facilitate downstream characterizations of generated affinity proteins. Taken together, in these studies we have demonstrated that the staphylococcal display system is a powerful alternative to existing technologies for protein engineering and protein characterization.
QC 20100809
APA, Harvard, Vancouver, ISO, and other styles
4

Sharav, Tumenjargal. "Tumor-spezifische T-Zellen und T-Zellepitope bei kutanen Lymphomen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2005. http://dx.doi.org/10.18452/15220.

Full text
Abstract:
Ziel dieser Arbeit war die Identifizierung von Tumor-assozierten T-Zellepitopen (TATE) in kutanen T-Zelllymphomen (CTCL) und die Charakterisierung der Tumour-spezifischen zytotoxischen Immunabwehr. Zwei Tumor-spezifische T-Zellklone wurden aus den Tumor-infiltrierenden Lymphozyten (TIL) eines CTCL-Patienten etabliert. Die potentiellen natürlichen T-Zellepitope und Mimotope (synthetische Epitope ohne natürliche Korrelate aber mit meist hohen T-Zellaktivitäten) für diese Klone wurden mit einer kombinatorischen Peptidbibliothek identifiziert. Die Qualität und Quantität der T-Zellaktivitäten waren bei den jeweiligen Peptiden unterschiedlich. Die funktionellen Aviditäten varierten dabei um 3 Größenordnungen. Bei den einzelnen Peptiden korrelierte die Zytolyse und Zytokinsekretion nicht immer. Mit einigen Mimotopen wurden CTCL-Patienten für therapeutische Zwecke vakziniert. Die Frequenzen der Mimotop-spezifischen T-Zellen erhöhten sich während der ersten Vakzinationszyklen und tumorizide Aktivitäten konnten nachgewiesen werden. Diese ersten klinischen Anwendungen der Mimotope zeigen die Möglichkeit solcher Mimotope die sonst unzureichende Immunabwehr zu modulieren. Die Identifizierung neuer TATE ermöglichte die weitere Untersuchung der Tumor-spezifischen T-Zellen in der Peripherie und im Tumor des Patienten. Diese Analysen zeigten, dass diese Zellen im peripheren Blut aktiv aber im Tumor inaktiv waren. Die TILs waren vom effektor-memory Phänotyp expremierten aber nur schwach oder z. T. keine der Moleküle mit Effektorfunktion. Das immunsuppressive Zytokin TGF-beta könnte eine wichtige Rolle bei dieser unzureichenden Immunabwehr bei CTCL spielen.
The major goals of this work was the identification of tumour-associated T cell epitopes (TATE) in cutaneous T cell lymphoma (CTCL) and the characterisation of the tumour-specific cytolytic immune response. Two tumour-specific cytolytic T cell clones were established from the tumour-infiltrating lymphocytes (TIL) of one CTCL-patient. The potential natural T cell epitopes and mimotopes (epitopes without natural correlates but with more T cell stimulating capacity) for these T cell clones were identified using a combinatorial peptide library. The quantity and quality of the T cell response was different. The functional avidity of the peptides differed more than 3 orders of magnitude. The cytolysis and cytokine release did not correlate for each peptide. Some of the mimotopes were injected into CTCL-patients for therapeutic purpose. The frequency of the mimotope-specific T cell increased during the first vaccination cycles and a tumouricidal capacity could be observed. This first clinical application of the mimotopes showed the capacity of the mimotopes for the modulation of weak anti-tumour immune response. The identification of the new TATE allowed further characterisation of the tumour-specific T cells in the periphery and in the tumour of the patient. High frequency of the tumour-specific T cells could be detected in the tumour but they failed to show effector functions in comparison to the tumour-specific T cells in the peripheral blood. The tumour-specific T cells had the effector memory phenotype but expressed none or less amount of the cytolytic effector molecules. The reason for the suboptimal anti-tumour response in CTCL could be the immunesuppressive cytokine TGF-beta.
APA, Harvard, Vancouver, ISO, and other styles
5

McMahan, Rachel H. "Relating TCR-peptide-MHC affinity to immunogenicity for the design of tumor vaccines /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2007.

Find full text
Abstract:
Thesis (Ph.D. in Immunology) -- University of Colorado Denver, 2007.
Typescript. Includes bibliographical references (leaves 133-156). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;
APA, Harvard, Vancouver, ISO, and other styles
6

Ahsendorf, Henrike Verfasser], Bertram [Akademischer Betreuer] [Brenig, Bertram [Gutachter] Brenig, Christiane [Gutachter] Stahl-Hennig, and El Wahed Ahmed [Gutachter] Abd. "Selection and characterization of human recombinant antibodies against Orthopoxviruses from an immunoglobulin library and mapping of functional epitopes of Vaccinia virus surface proteins / Henrike Ahsendorf ; Gutachter: Bertram Brenig, Christiane Stahl-Hennig, Ahmed Abd El Wahed ; Betreuer: Bertram Brenig." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1208221833/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Ahsendorf, Henrike [Verfasser], Bertram [Akademischer Betreuer] Brenig, Bertram [Gutachter] Brenig, Christiane [Gutachter] Stahl-Hennig, and El Wahed Ahmed [Gutachter] Abd. "Selection and characterization of human recombinant antibodies against Orthopoxviruses from an immunoglobulin library and mapping of functional epitopes of Vaccinia virus surface proteins / Henrike Ahsendorf ; Gutachter: Bertram Brenig, Christiane Stahl-Hennig, Ahmed Abd El Wahed ; Betreuer: Bertram Brenig." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1208221833/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Tseng, Sy-Woei, and 曾斯偉. "Construction of phage-display peptide library for the screening of antibody epitope." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/04418277385769813527.

Full text
Abstract:
碩士
國立中興大學
分子生物學研究所
88
Abstract Phage display technology, consisting of the expression of target sequence in the surface of phage particles and the affinity selection, is a biological system that facilitates the cloning and rapid selection of peptides or proteins from large combinatorial libraries. Libraries are generated by cloning of a batch of DNA encoding millions of variants of certain ligands into the phage genome or phagemid as a fusion to the gene encoding one of the phage coat proteins. Upon expression, the fusion coat protein is incorporated into new phage particles that are assembled in the bacterium and consequently presented on the phage surface, with its genetic material residing within the phage particles. The purpose of this study is to construct a random peptide library containing both linear and conformational epitopes to serve as an “all purpose” peptide source for searching of disease-related peptides. To achieve this goal, EcoRI-HindIII DNA fragments encoding 45 amino acid residues, of which 36 have random sequences, were cloned into the corresponding sites of the modified pCANTAB5E plasmid and the resulted plasmids were transformed into E. coli TG1. The percentage of insert-containing colonies in the transformants was evaluated by PCR using primers flanking the insert DNAs. Approximately, 83% of the clones contained inserts. The expression of the insert gene was determined by colony immunoblot analysis of a portion of the original clones using the antibody specific for E-Tag which is located downstream of the insert. The number of clone capable of expression coincided with the percentage of the insert-containing clones. Finally, the diversity of the library was evaluated by sequencing of 10 randomly picked clones. The results indicated that all exhibited difference sequences. The diversity of the library was estimated to be 4.4 x 106 according to the above information,. To establish a system for identification of disease-related peptides, affinity selection was performed using sera from system lupus erythematosus patients.
APA, Harvard, Vancouver, ISO, and other styles
9

Rai, Chung-I., and 雷仲益. "Identification of the epitope recognized by enhancing monoclonal antibody against dengue virus using phage-displayed library." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/59300346968131685111.

Full text
Abstract:
碩士
國立成功大學
醫學檢驗生物技術學系
94
Dengue virus (DV), consists of four serotypes (DEN1-4), is the causative agent of mild dengue fever and lift-threatening dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS). The antibody dependent enhancement (ADE) hypothesis has been proposed to explain why more severe manifestations of DHF/DSS occur predominantly during secondary infection with different serotypes. However, the epitopes recognized by these enhancing antibodies are unclear. Recently, anti-prM monoclonal antibody (mAb 70-21) which recognized all DV serotypes without neutralizing activity were generated and demonstrated as an enhancing antibody for DV infection. In this study, the epitope recognized by mAb 70-21 was identified using a phage-displayed random peptide library. After three rounds of biopanning, immunopositive phage clones showed specific binding to mAb 70-21 but not to normal mouse serum or purified normal mouse IgG by ELISA. DNA sequencing and GCG analysis of these phage clones showed a consensus sequence, QNNLGPR, which shared sequence homology to the amino acid 58-61 of dengue prM protein and N-terminus of heat shock protein 60. Moreover, phage clones induced-antisera recognized not only these clones but also synthetic peptide of the consensus sequence. In addition, phage-induced antisera also bound to normal Vero or RD cells as demonstrated by indirect fluorescent assay. Western blot analysis and immunoprecipitation showed heat shock protein 60 in BHK-21 cell lysate was recognized by phage clones induced-antisera. Like mAb70-21, these phage-induced antisera also could enhance dengue virus infection of BHK-21 cells. Moreover, antibodies against QNNLGPR synthetic peptide were significantly increased in dengue patients with severe syndrome. Taken together, our results suggest that antibodies which recognized the epitope shared by prM of DV and heat shock protein 60 may enhance DV infection of host cells, which may play a role in the development of DHF or DSS.
APA, Harvard, Vancouver, ISO, and other styles
10

Ahsendorf, Henrike. "Selection and characterization of human recombinant antibodies against Orthopoxviruses from an immunoglobulin library and mapping of functional epitopes of Vaccinia virus surface proteins." Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0005-137B-C.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Epitope library"

1

Renatus, Flavius Vegetius. Epitoma rei militaris. Stutgardiae: B.G. Teubner, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

P, Milner N., ed. Vegetius, epitome of military science. Liverpool: Liverpool University Press, 1993.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Renatus, Flavius Vegetius. Vegetius : epitome of military science. Liverpool, [Eng.]: Liverpool University Press, 1993.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

1925-, Stelten Leo F., ed. Epitoma rei militaris. New York: P. Lang, 1990.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Renatus, Flavius Vegetius. Epitoma rei militaris. Oxford: Clarendon Press, 2004.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Renatus, Flavius Vegetius. Epitoma rei militaris =: Das gesamte Kriegswesen. Aarau: Sauerlander, 1986.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Renatus, Flavius Vegetius. The earliest English translation of Vegetius' De re militari. Heidelberg: C. Winter Universitätsverlag, 1988.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

1892-, Phillips Thomas Raphael, ed. The military institutions of the Romans. Westport, CT: Greenwood Press, 1985.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Renatus, Flavius Vegetius. Le livre de l'art de chevalerie de Vegesce: Traduction anonyme de 1380. Helsinki: Suomalainen Tiedeakatemia, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Renatus, Flavius Vegetius. Compendio delle istituzioni militari. 2nd ed. Catania: Edizioni del Prisma, 1997.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Epitope library"

1

Midoro-Horiuti, Terumi, and Randall M. Goldblum. "Epitope Mapping with Random Phage Display Library." In Methods in Molecular Biology, 477–84. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-992-5_28.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Vanniasinkam, Thiru, Mary D. Barton, Tongted Phumoonna Das, and Michael W. Heuzenroeder. "B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay." In Epitope Mapping Protocols, 121–28. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7841-0_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Smith, George P., and Jamie K. Scott. "Using an epitope library to identify peptide ligands for antibodies against folded epitopes." In Peptides, 485–88. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2264-1_186.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Volkmer-Engert, R., J. Hellwig, B. Ehrhard, W. Höhne, and J. Schneider-Mergener. "Characterization of the interaction between the monoclonal antibody CB 4-1 and its peptide epitope GATPQDLNTM using a soluble and a cellulose-bound peptide epitope library." In Peptides 1994, 473–74. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-1468-4_213.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Wen, Fei, Mason R. Smith, and Huimin Zhao. "Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification." In Methods in Molecular Biology, 213–34. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9597-4_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Wen, Fei, and Huimin Zhao. "Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification." In Methods in Molecular Biology, 245–64. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-589-7_15.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Bent, Margaret. "25.Washington, Library of Congress, M2.1 .C6 1400 Case: A Neglected English Fragment." In Epitome musical, 529–52. Turnhout, Belgium: Brepols Publishers, 2022. http://dx.doi.org/10.1484/m.em-eb.5.132718.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Gembero-Ustárroz, María. "4. Music Books for Lima Cathedral and their Social Context in the Early Seventeenth Century: Black Slaves as a Guarantee for Producing a New Plainchant Library." In Epitome musical, 105–26. Turnhout, Belgium: Brepols Publishers, 2021. http://dx.doi.org/10.1484/m.em-eb.5.129719.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Brahma, Sandipan, and Steven Henikoff. "CUT&RUN Profiling of the Budding Yeast Epigenome." In Methods in Molecular Biology, 129–47. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2257-5_9.

Full text
Abstract:
AbstractMapping the epigenome is key to describe the relationship between chromatin landscapes and the control of DNA-based cellular processes such as transcription. Cleavage under targets and release using nuclease (CUT&RUN) is an in situ chromatin profiling strategy in which controlled cleavage by antibody-targeted Micrococcal Nuclease solubilizes specific protein-DNA complexes for paired-end DNA sequencing. When applied to budding yeast, CUT&RUN profiling yields precise genome-wide maps of histone modifications, histone variants, transcription factors, and ATP-dependent chromatin remodelers, while avoiding cross-linking and solubilization issues associated with the most commonly used chromatin profiling technique Chromatin Immunoprecipitation (ChIP). Furthermore, targeted chromatin complexes cleanly released by CUT&RUN can be used as input for a subsequent native immunoprecipitation step (CUT&RUN.ChIP) to simultaneously map two epitopes in single molecules genome-wide. The intrinsically low background and high resolution of CUT&RUN and CUT&RUN.ChIP allows for identification of transient genomic features such as dynamic nucleosome-remodeling intermediates. Starting from cells, one can perform CUT&RUN or CUT&RUN.ChIP and obtain purified DNA for sequencing library preparation in 2 days.
APA, Harvard, Vancouver, ISO, and other styles
10

Moret, E. E., R. M. J. Liskamp, and J. P. Tollenaere. "Structure-based design of epitope mimetics." In Pharmacochemistry Library, 371–81. Elsevier, 1997. http://dx.doi.org/10.1016/s0165-7208(97)80079-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Epitope library"

1

LOFTUS, J. C., E. F. Plow, A. L. Frelinger III, M. A. Smith, S. D’ouza, and M. H. Ginsberg. "LOCALIZATION AND CHEMICAL SYNTHESIS OF A DIVALENT CATION REGULATED EPITOPE IN PLATELET MEMBRANE GPIIb." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643959.

Full text
Abstract:
Platelet membrane glycoprotein (GP)IIb-IIIa is a component of a common adhesive protein receptor for fibrinogen, fibronectin, and von Willebrand factor. A monoclonal antibody, PMI-1, defines a divalent cation dependent regulation of the surface orientation of the heavy chain of GPIIb. Exposure of the PMI-1 epitope inversely correlates with the capacity of platelets to bind fibrinogen and aggregate. We have now localized and chemically synthesized this epitope. A 1.1 Kb cDNA clone which directs the synthesis of a fusion protein which bears the PMI-1 epitope was isolated from a lambda gt 11 expression library constructed from mRNA from the human erythroleukemia (HEL) cell line. The position of the N-terminal sequence of the light chain of GPIIb in the deduced amino acid sequence of the clone defined the orientation of the light and heavy chains of GPIIb. Analysis of the amino acid sequence corresponding to the heavy chain of GPIIb identified a single region with a high likelihood of containing a continuous epitope. A synthetic 17 residue peptide, corresponding to the predicted antigenic site, inhibited the binding of PMI-1 to platelets. Two uM peptide was required to inhibit binding 50% in the presence of 1 uM PMI-1, indicating an approximate dissociation constant of 1.5 uM for the peptide-antibody complex. This figure should be compared to a Kd of 0.95 uM (JCI 78:1103, 1986) for PMI-1 binding to GPIIb. A second peptide, corresponding to the region immediately adjacent to the predicted antigenic site, failed to inhibit PMI-1 binding. Neither peptide inhibited the binding of two other monoclonal anti GPIIb-IIIa’s to platelets. The peptides had similar effects on PMI-1 interaction with purified GPIIb-IIIa in detergent solution. These data localize the PMI-1 epitope to a 17 amino acid region located near the carboxyl terminal of the heavy chain of GPIIb. Thus, they chemically define a region of GPIIb whose surface expression reflects the competence of GPIIb-IIIa as a component of a platelet receptor for adhesive proteins.
APA, Harvard, Vancouver, ISO, and other styles
2

Lu, Yang, Yongpeng Xiao, Qiao Li, and Man Liu. "An introduction to main B-cell epitope prediction methods and software based on phase display library." In 2011 23rd Chinese Control and Decision Conference (CCDC). IEEE, 2011. http://dx.doi.org/10.1109/ccdc.2011.5968954.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Morrissey, J. H., D. S. Fair, and T. S. Edgington. "STRUCTURE AND PROPERTIES OF THE HUMAN TISSUE FACTOR APOPROTEIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643738.

Full text
Abstract:
Tissue factor (TF), an integral membrane glycoprotein, is an initiating molecule for the coagulation protease cascade. TF must reside in a phospholipid membrane for optimal activity where it functions as the receptor and essential allosteric activator for factor Vll/VIIa.TF apoprotein was purified from human brain and placenta using factor Vll-affinity chromatography or immunoaffinity chromatography with a mouse anti-TF monoclonal antibody. Both methods resulted in a homogeneous preparation consisting of a highly glycosylated 47 kDa heavy chain and a 12.5 kDa light chain.Removal of asparagine-linked carbohydrate chains with glycopeptidase F reduced the apparentmolecular weight of the heavy chain to 37 kDa but hadno effect on the mobility of the light chain in SDS gel electrophoresis. Electrophoretic analysis of theintact protein with and without reduction indicates that the light chain is disulfide-linked to the heavychain in about half of the TF molecules and is notessential for function.The majority of polyclonal andtwenty- nine monoclonal antibodies against purified TF strongly inhibit coagulation and in all cases aredirected against epitopes on the heavy chain alone. Functional regions of the TF heavy chain have been investigated using a library of twenty-nine monoclonalantibodies and a series of overlapping, synthetic oligopeptides based on sequence information obtained from cloning the cDNA for TF. Supported by NIH grantsHL-16411 and CA-41085.
APA, Harvard, Vancouver, ISO, and other styles
4

Murata, Kenji, Kayoko Saso, Linh T. Nguyen, Douglas Millar, Marcus O. Butler, Pamela S. Ohashi, and Naoto Hirano. "Abstract 568: Decoding shared antigenic epitopes and their cognate TCR genes in melanoma TILs using a library of paired human cell-based pHLA multimers and artificial APCs." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-568.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Murata, Kenji, Kayoko Saso, Linh T. Nguyen, Douglas Millar, Marcus O. Butler, Pamela S. Ohashi, and Naoto Hirano. "Abstract 568: Decoding shared antigenic epitopes and their cognate TCR genes in melanoma TILs using a library of paired human cell-based pHLA multimers and artificial APCs." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-568.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Marquerie, G., A. Duperray, G. Uzan, and R. Berthier. "BIOSYNTHETIC PATHWAYS OF THE PLATELET FIBRINOGEN RECEPTOR IN HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642954.

Full text
Abstract:
Interaction between cells and between cells and extracellular matrices are critical for a number of biological processes, including organ development, cell differenciation, cell motility, and the inimune' response. These interactions are mediated by a family of adhesion receptors that recognize short sequences such as Arg-Gly-Asp (RGD). These receptors share similar structural properties. They are heterodimers composed of a and B subunits and sometime express common epitopes. This suggests that the structural and functional relationship of these receptors may result from the transcription of related genes or may arise from cell specific post-transcriptional events. Thus, analysis of the biosynthesis and processing of these receptors would provide valuable insights into the molecular mechanism which control their expression at the surface,of different cells. Platelet membrane glycoprotein (GP) IIb-IIIa is a member of this receptor family. This protein is a non covalent heterodimer composed of two distinct polypeptides, Glib which consists of two subunits Ilba and IlbB (Mr = 116 kD, Mr = 25 kD) and GPIIIa (Mr = 100 kD, reduced). GPIIb-IIIa functions at site of platelet aggregation and serves as receptor for RGD containing factors including fibrinogen, fibronectin and von Willebrand factor. We report here on the investigation of the biosynthetic pathways of this RGD receptor in human megakaryocytes. High number of megakaryocytic cells from the megakaryoblastic stage to the polyploid mature megakaryocyte were obtained from liquid culture of cryopreserved leukocyte stem cell concentrates from patients with chronic myelogenous leukemia (CML). After sorting, using a FACS IV and indirect immunofluores-cent labeling with monoclonal antibodies anti-GPIIb-IIIa, 95 % of the cells in culture were of the megakaryocytic lineage. These megakarocytes represented an excellent tool to delineate at the molecular level events associated with the biosynthesis of GPIIb-IIIa.Metabolic labeling and pulse-chase experiments indicated that GPIIb and GPIIIa are synthesized from separate mRNA and that the two subunits of GPIIb derive from a common precursor. This was further confirmed by cell-free translation of megakaryocyte mRNA and the identification of separate cDNA containing sequences coding for the pro-GPIIb and for GPIIIa. These cDNA were isolated from a Xgt11 expression library constructed with purified megakaryocyte RNA, and were used to size the messengers coding for the two polypeptides. A single mRNA species of 3.9 kB was found to encode the pro-GPIIb, whereas two different mRNA species of 2.9 kB and 4. 1 kB were identified with the GPIIIa cDNA.The newly synthesized GPIIIa associates early with the pro-GPIIb in the rough endoplasmic reticulum. Examination of the glycosylation pathways with endoglycosidase H, tunicamycin and monensin indicated that high mannose oligosaccharides are added to the GPIIIa and pro-GPIIb polypeptide backbone. The pro-GPIIb is then processed with conversion of high mannose to the complex type carbohydrate, whereas GPIIIa remains endoH sensitive. Glycosylation of pro-GPIIb-IIIa and processing of oligosaccharides are prerequisite for proteolytic maturation of pro-GPIIb and the expression of the mature complex at the surface of the cell. Thus post-translational processing of GPIIb-IIIa requires an early assembly of the complex. This may have important implications in the maturation of megakaryocyte granules and in the molecular mechanism underlying the Glanzmann thrombastenic disease.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Epitope library' for particular source types:
Journal articles Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography