Dissertations / Theses on the topic 'Epithelium Cultures and culture media'
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Thornton, Sarah. "Record hops to raves : authenticity and subcultural capital in music and media cultures." Thesis, University of Strathclyde, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261836.
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Tsoulis-Reay, Alexa. "Convergence, concern & the "real" girl : teenage girls' everyday media cultures /." Connect to thesis, 2009. http://repository.unimelb.edu.au/10187/4893.
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Rivas, Cecilia Maribel. "Imaginaries of transnationalism media and cultures of consumption in El Salvador /." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2007. http://wwwlib.umi.com/cr/ucsd/fullcit?p3258783.
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Thesis (Ph. D.)--University of California, San Diego, 2007.Title from first page of PDF file (viewed June 8, 2007). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 159-168).
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林結美 and Kit-mei Lam. "Phenotypic and molecular characterization of a blood culture isolate of the family coriobacteriaceae." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42904274.
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Ferreira, Ana Raquel Santos. "A systems biology framework for pathway level culture media engineering: pplication to Pichia pastoris cultures." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/9369.
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Dissertação para obtenção do Grau de Doutor em Engenharia Química e BioquímicaCulture media (CM) formulations contain hundreds of ingredients in aqueous solutions that may be involved in complex interactions in the same or competing pathways within the cell. This thesis proposes a new methodology for determining the optimal composition of CM that migrates from an empirical to a mechanistic or hybrid mechanistic CM development approach. A framework consisting in the execution of an array of cell cultures, endpoint exometabolomic assays and bioinformatics algorithm were brought together into a platform for CM engineering called Cell Functional Enviromics. This technology consists of a largescale reverse engineering approach that reconstructs cellular function on the basis of measured dynamic exometabolome data. To support this concept, a computational algorithm, called “envirome-guided Projection to Latent Pathways”, was developed. This method yields envirome-wide Functional Enviromics Maps (FEM), with rows representing medium factors, columns representing elementary (orthogonal) cellular functions and color intensity values, the strength of up-/down- regulation of cellular functions by medium factors. This method was applied to optimize Pichia Trace Metal salts for the yeast Pichia pastoris to improve the expression of heterologous proteins. An array of shake flasks experiments of the P. pastoris X33 strain were performed and used to build a FEM. Then, optimized CM formulations were calculated targeting predefined single-chain Fragment variable antibody (scFv) production improvements. Experimental validation shows a scFv productivity increase of approximately twofold, in relation to the control BSM recipe proposed by Invitrogen. These results were further validated in 2 L bioreactor experiments. Thereafter, scale-up to 50 L bioreactors was developed a mathematical model for further optimization of BSM salts in experiments of P. pastoris GS115. Direct adaptive (DO)-stat feeding controller that maximizes glycerol feeding through the regulation of DO concentration at 5% of saturation was developed and applied to the 50 L bioreactor, with the fully optimized CM composition.
Fundação para a Ciência e Tecnologia - bolsa de doutoramento SFRH/BD/36285/2007
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Pierre-Charles, Nathalie. "La construction du sens chez des jeunes de cultures diverses lors de la réception de messages médiatiques." Thesis, Paris 13, 2014. http://www.theses.fr/2014PA131043/document.
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Cette étude sur la compréhension des jeunes face aux messages médiatiques vise : - à mettre en évidence les apports des études de réception et plus précisément les capacités et les besoins des récepteurs médiatiques, le cadre de réception, la nature du public et les médiations socioculturelles ; - reconstituer la capacité, la compétence et l’intention communicationnelles du récepteur médiatique et la rhétorique de la réception ; - poser le cadrage pratique de notre étude par la nature scientifique et le type des messages proposés. Nous nous intéressons à la construction du sens par des jeunes de cultures diverses lors de la réception de messages scripturaux, audiovisuels et audio scriptovisuels. Nous traitons les données collectées par l’observation et le questionnement à la fois quantitativement et qualitativement. Les techniques de recueil de données retenues sont le questionnaire et l’observation. Problématiser notre objet de recherche revient à se poser la question suivante : quelle est la compréhension des jeunes face à la réception des messages médiatiques ? Nous organisons notre recherche autour de deux axes : - le jeune construit sa compréhension du message médiatique ; - le culturel et le social ont un impact sur la compréhension du message médiatique par le jeuneThis study on the understanding of young people facing media messages aims: -to highlight the contributions of reception studies and specifically capabilities and the needs of media receivers, the approval framework, the nature of the public andsociocultural mediations; -restore the capacity, competence and the communicative intention of the media receiverand rhetoric of reception; -ask the practical framing of our study by the scientific nature and type of the proposedposts. We are interested in the construction of meaning by young people from various cultures during the reception of messages scriptural, audio-visual and audio disposed. We treat data collected through observation and questioning both quantitatively and qualitatively. Selected data collection techniques are the questionnaire and observation.Problematize our object of research is to ask the following question: what is the understanding of young people in receipt of media messages? We organize our research around two axes: -the young built his understanding of the media message.-the cultural and social impact on the understanding of the media message by the young
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Zimm, Malin. "Losing the plot : architecture and narrativity in fin-de siècle media cultures." Doctoral thesis, KTH, Architecture, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-481.
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This thesis investigates the role of the term plot in mediating relations between architecture and narrativity. Examining organisational strategies in the creation of real and virtual spaces, it identifies literary works by novelists who have resisted, or subverted, plot conventions in fiction (Joris-Karl Huysmans, Edmond de Goncourt, Xavier de Maistre and Neal Stephenson), and introduces architectural spaces such as Thomas Edison’s film-studio Black Maria, and the plotless productions of early cinematography, to juxtapose concepts of plot and spatiality in a study of the production and consumption of pre-digital virtual spaces. Plot here relates therefore both to narrative sequentiality and spatial organisation – from "storyline" to "ground plan". The "plotless" narrative structure of Huysmans, Goncourt and de Maistre focuses on the interaction between man – the "writerin- residence" – and his domestic interior, functioning as an excitant or stimulant for the production of both material and imagined spaces. The media culture of late 19th century society saw the first significant attempts at moving image technology and its related spatialities – the Black Maria, the kinetoscope, the kinetograph, and the films produced by these, which had yet to find a narrative form. The architecture of the plotless novels and the proto-cinematic experiments of the late 19th century modulate between physical reality and fiction. They are ripe in their descriptive narrativity, expanding in the imagination of the consumer. Stephenson’s imaginative transposition of book media into a "Primer" – a new form of narrative media that develops its narrative content directly from the environmental context of its reader – concludes the discussion of the thesis, highlighting interrelations between fictive and real space, influencing both writer and reader. The refusal of narrative plot deprives the reader of causality, but emphasises the fictitious spatial creation in which the reader becomes immersed. These spaces, by virtue of their disengagement from plot, allow us to revisit the possibilities of virtual space without common preconceptions concerning the creation or experience of digital mediating technology.
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Wonnah, Samson. "Myths, Risks, and Ignorance: Western Media and Health Experts’ Representations of Cultures in Ebola-Affected West African Communities." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3389.
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The 2014 Ebola outbreak, mostly affecting Liberia, Sierra Leone, and Guinea, is the largest ever recorded. The Ebola response encountered resistance in some affected communities, where some residents accused relief agencies from the Global North of denigrating local cultures. This thesis examines mainstream Western media and health experts’ representation of culture in the Ebola-affected region and employed Foucauldian analysis of discursive power to discuss the impact of such a representation on the concerned communities. Through a content analysis of selected journal and news articles by Western scholars and media and official reports by some relief agencies involved with the Ebola response, the study discovers evidence of culture bias. There was a use of significantly negative words in describing aspects of culture in the Ebola-affected region. Western media and health experts also largely associated the epidemic with African “backwardness.”
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Rogan, Frances. "Social media, bedroom cultures and femininity : exploring the intersection of culture, politics and identity in the digital media practices of girls and young women in England." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/8199/.
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In recent years, the position of (post-)millennial girls and young women within the digital landscape of social media has proven to be a topic of much interest to a number of feminists, journalists and cultural commentators. On the one hand, girls’ (social) media practices are presented as a key site of concern, wherein new digital technologies are said to have produced an intensification of individualized, neoliberal and post-feminist identities. At the same time, others have championed access to social media for young people as a revolutionary political tool, wherein previously marginalised political subjects (such as girls) can access and participate within new and exciting political cultures. This thesis offers an original contribution to these debates by locating itself at the intersection of these two approaches and examining the role of social media in the production of girls’ cultural and political identities. I present my findings from focus groups carried out with girls (aged 12-18) in three urban locations in England. This data is organised around the three overriding themes of space, surveillance and visibility. Ultimately, the thesis argues that social media should be conceptualised as an important terrain upon which neoliberal and postfeminist subjectivities can be both reproduced and subverted.
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Szemiel, Agnieszka M. "Replication of Bunyamwera virus in mosquito cells." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2570.
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The Bunyaviridae family is one of the largest among RNA viruses, comprising more than 350 serologically distinct viruses. The family is classified into five genera, Orthobunyavirus, Hantavirus, Nairovirus, Phlebovirus, and Tospovirus. Orthobunyaviruses, nairoviruses and phleboviruses are maintained in nature by a propagative cycle involving blood-feeding arthropods and susceptible vertebrate hosts. Like most arthropod-borne viruses, bunyavirus replication causes little damage to the vector, whereas infection of the mammalian host may lead to death. This situation is mimicked in the laboratory: in cultured mosquito cells no cytopathology is observed and a persistent infection is established, whereas in cultured mammalian cells orthobunyavirus infection is lytic and leads to cell death. Bunyaviruses encode four common structural proteins: an RNA-dependent RNA polymerase, two glycoproteins (Gc and Gn), and a nucleoprotein N. Some viruses also code for nonstructural proteins called NSm and NSs. The NSs protein of the prototype bunyavirus, Bunyamwera virus, seems to be one of the factors responsible for the different outcomes of infection in mammalian and mosquito cell lines. However, only limited information is available on the growth of bunyaviruses in cultured mosquito cell lines other than Aedes albopictus C6/36 cells. Here, I compared the replication of Bunyamwera virus in two additional Aedes albopictus cell clones, C7-10 and U4.4, and two Aedes aegypti cell clones, Ae and A20, and investigated the impact of virus replication on cell function. In addition, whereas the vertebrate innate immune response to arbovirus infection is well studied, relatively little is known about mosquitoes’ reaction to these infections. I investigated the immune responses of the different mosquito cells to Bunyamwera virus infection, in particular antimicrobial signaling pathways (Toll and IMD) and RNA interference (RNAi). The data obtained in U4.4 cells suggest that NSs plays an important role in the infection of mosquitoes. Moreover infection of U4.4 cells more closely resembles infection in Ae and A20 cells and live Aedes aegypti mosquitoes. My data showed that the investigated cell lines have various properties, and therefore they can be used to study different aspects of mosquito-virus interactions.
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Kavanaugh, Rathi G. "Investigation of bacterial populations in a biological nutrient removal system." Diss., Virginia Tech, 1991. http://hdl.handle.net/10919/39828.
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Bacterial populations proliferating in a pilot scale biological nutrient removal system (BNR) were studied. The objective of the research was to develop media and methods to identify bacterial populations in BNR systems. Samples were obtained from the last aerobic zone of a University of Cape Town (UCT)-type system. The most probable numbers (MPN) of bacteria in the samples were analyzed in liquid media containing volatile fatty acids as sole sources of carbon. Samples were also transferred to denitrification medium, and MPN's of denitrifiers were recorded. The growth in liquid medium was plated on solid medium. Gram-negative cultures were isolated and identified. The phosphorus-removal capacity of five isolates also was studied.
The results indicated that several different genera of bacteria are involved in the removal of phosphorus in an operating BNR system. Four major groups of phosphorus storing bacteria, Aeromonas/Vibrio, coliforms, Pseudomonas spp and Acinetobacter spp, were recovered. The identification of cultures on denitrification medium also recovered Pseudomonas, Aeromonas, coliforms and Acinetobacter, indicating the overlap in the function of these genera. The phosphorus accumulations in three of the tested cultures showed accumulations in excess of 10 percent.
The MPN's of bacteria in acetate and propionate media obtained using samples from the pilot scale BNR system and a full scale activated sludge system were statistically analyzed. The analyses showed significant differences between MPN in acetate and propionate medium using samples from the BNR system, whereas there were no significant differences in samples from the conventional activated sludge plant. The possibility of the application of these data in process control and modeling is proposed.Ph. D.
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Gustafsson, Stina, and Therese Högberg. "“In the footsteps of globalization” : A media ethnographic study about Ethiopian journalism students’ perceptions about television’s influence on their identities and on cultures in Ethiopia." Thesis, Linnéuniversitetet, Institutionen för medier och journalistik (MJ), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-25724.
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This is a media ethnographic bachelor thesis. It examines journalism students at Bahir Dar University’s perceptions about how television can influence their identities but also cultures and lifestyles in Ethiopia in general. Ethnographic observations, time-use diaries, group interviews and individual interviews were used to gather data. However, the individual interviews are our main method. Our results showed that the journalism students believe that television influence them in various ways: their way of dressing and looking, acting, thinking and being. They also believe that televisions’ ability to change their identities in a bigger context means that cultures and traditions in Ethiopia are changing. The journalism students talk about different phenomenons that have arisen in Ethiopia during the last years, for example homosexuality, new techniques and appliances and new kind of clothes and food. And they say that they believe these phenomenons have arisen in Ethiopia because of media globalization.
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Du, Plooy Daniel Rupert. "Mediated identity construction across cultures : an analysis of reports on the Guguletu Seven." Thesis, Stellenbosch : University of Stellenbosch, 2008. http://hdl.handle.net/10019.1/1841.
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Thesis (MPhil (General Linguistics))--University of Stellenbosch, 2008.This thesis has been written as a research project within a programme that topicalises intercultural communication in fairly broad terms. It provides an analysis of the different constructions in the media of events and people by journalists from different linguistic communities who have regular intercultural contact in the course of reporting on local newsworthy events. The communities here are different media producers, different news publishing institutions who print and circulate current news to audiences in different language communities. Illustratively, attention will go to the particular role players in the media, i.e. news producers (journalists, newspapers, publishing groups), newsmakers (people whose actions are observed and topicalised in the media) and news consumers (the audience, readership) engaged in reporting on a particular, prominently mediated event in 1986, and again in 1996. The event that is now recorded as the Guguletu Seven incident is investigated for the way in which it can highlight cultural linguistic differences in mediating the same event.
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Daste, Sophie. "Expérimentations artistiques des espaces de fiction générés par les nouveaux médias issus des cultures générationnelles otaku et geek." Thesis, Paris 8, 2015. http://www.theses.fr/2015PA080042.
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Dans un contexte généralisé où les cultures générationnelles otaku et geek multiplient leurs influences autant sur le plan du contenu culturel que sur les nouveaux médias qu’elles génèrent, nous nous sommes intéressés à sa propension à pénétrer les champs des arts dont la pratique contemporaine. La convergence des médias inhérents à ces deux cultures produit de nouveaux moyens d’expression donnant naissance à plusieurs types de pratiques expérimentales. Il s’est agi pour nous d’interroger nos référents autant que notre pratique sous ce nouvel angle. Une émergence de nouveaux systèmes technologiques se démocratise et intervient de plus en plus fréquemment dans le quotidien des usagers. Sur la base de ces nouvelles connaissances se développent de nouveaux rapports aux objets à partir desquels se forment de nouveaux espaces de fiction.La thèse « Expérimentations artistiques des espaces de fiction générés par les nouveaux médias issus des cultures générationnelles otaku et geek » est transdisciplinaire. Elle mêle études étymologiques, anthropologiques et sociologiques à l’étude des arts. En plus de s'appuyer sur des études réalisées par des chercheurs, la méthodologie appliquée se fonde sur l’expérimentation des œuvres présentées qui touchent à la fois à l’art contemporain, aux livres, aux bandes dessinées, aux films, aux jeux vidéo, aux jeux à réalité alternée, etc., mais aussi à la convergence de tous ces médias. L’expérimentation par une pratique nous semble dans le domaine présenté une donnée très importante du bien-fondé de la recherche qui est basée sur l’étude d’un terrain culturel en constante évolutionIn a widespread background in which the influences of Otaku and Geek Generational Cultures have increased as regards both their cultural content and the new media created by them, we undertook to study their propensity to penetrate into the art sphere, whether within or outside contemporary art. The convergence of the media inherent to both cultures has produced new means of expression which have given rise to several types of experimental practices. In the light of these new aspects, we strove to review our referents as well as our practice. Emerging new IT systems are becoming more and more popular and they are increasingly affecting their users' daily lives. With such new items of knowledge, new relations to things have developed and they provide a foundation on which new Fantasy Realms are being built."Artistic Testings of Fantasy Realms Created by New Media derived from the Otaku and Geek Generational Cultures" is a transdisciplinary thesis. It blends art research with etymological, anthropological and sociological studies. The methodology applied relies on studies carried out by researchers and is based on the testing of the works presented which are related to contemporary art, books, comics, video games, alternate reality games etc., as well as to the convergence of all such media. As regards the domain referred to herein, we believe that testing through practising is a paramount aspect of the relevance of this thesis which is based on the study of an ever evolving cultural territory
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Da, Serra Maria Fatima. "Fungal and substrate-associated factors affecting lignocellulolytic mushroom cultivation on wood sources available in South African [i.e. Africa]." Thesis, Rhodes University, 1997. http://hdl.handle.net/10962/d1004080.
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Vast- quantities of lignocellulosic materials, representing potential substrates for the cultivation of speciality mushrooms, are produced annually in South Africa. A number of these materials are derived as waste products of the timber and agricultural industries, e.g. Maranti (Shorea spp.) and Port Jackson Willow (Acacia longifolia) respectively. The screening of various wood-degrading fungi, which are cultivated worldwide for their production of speciality mushrooms, indicated that under the environmental conditions considered, certain species were adapted to cultivation on these lignocellulosic wastes (Pleurotus species) whereas others were not (Lentinus edodes and Flammulina velutipes). Furthermore, intra- and interspecies specific differences in the growth and production potential of the various lignocellulolytic fungi investigated on synthetic and natural medium were discovered. Biochemical and genetical investigations of these strains indicated differences between and within species which were often significant. Species varied qualitatively and quantitatively in the lignocellulolytic enzymes produced, which was loosely correlated with productivity on the different media investigated. Genetical studies, using RAPD fingerprinting, indicated that the Pleurotus genus is highly variable which supports the observed differences in growth, yield and enzymatic activity between different strains and species.
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Ortmanis, Andris. "The development of a microcomputer controlled variable pathlength turbidimeter /." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65460.
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Rossi, Rossana Cassanta. "Patrolando juventudes: o caderno Patrola ensinando jovens a consumir." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2007. http://hdl.handle.net/10183/11093.
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Esta Dissertação tem como objetivo desconstruir discursos acerca dos modos de ser jovem na contemporaneidade articulados ao consumo como prática social. Compreendo que é através das estratégias engendradas no âmbito da cultura do consumo que objetos, imagens, desejos, identidades, valores, modos de ser podem ser transformados em mercadorias: podem ser ‘adquiridos’, consumidos e por fim descartados. Tornam-se ‘objetos’ a serem usados e exibidos. Entre tantos artefatos que circulam e são produzidos nessa cultura de consumo, está o Caderno Patrola, encartado no jornal Zero Hora – jornal de maior circulação no Estado do Rio Grande do Sul. Assim, realizo uma leitura (entre tantas possíveis) a respeito de mídia, cultura de consumo e juventudes. De certa forma, é uma leitura que os Estudos Culturais, campo teórico no qual me movimento, possibilitam-me produzir. Para que esta Dissertação pudesse ser construída tal como está, o corpus de pesquisa se constituiu de diferentes materiais, a saber: seleção de edições do Caderno Patrola, pesquisas em diversos sites, incursões em comunidades virtuais do orkut e em blogs,, conversas com jovens leitores do Caderno através do Messenger e e-mails com a editora do Caderno. Nas análises, trago algumas reflexões a respeito dos modos de endereçamento do Caderno Patrola, discutindo algumas das estratégias do Caderno para interpelar os sujeitos jovens para suas páginas, bem como para convidá-los a consumir produtos que ‘anuncia’. Ainda, analiso o modo como os discursos do Caderno Patrola não apenas sugerem objetos de consumo que podem constituir certos modos de ser jovem, como também podem ensinar o que consumir para ‘possuir’ tais modos, para, assim, adotarse as ditas posições desejáveis de ser jovem. A partir das problematizações realizadas no decorrer do estudo acerca do Caderno Patrola, é possível compreender como se investe no promissor mercado jovem, não só fabricando produtos para eles como também transformando os produtos fabricados pelos jovens em algo rentável. Além disso, é possível constatar como as próprias culturas juvenis se tornam um produto, uma vez que muitos desejam ser, estar, permanecer jovem e, por isso, passam a consumir produtos ditos pertencentes a elas. Assim, através do potencial pedagógico do Caderno, procuro entender algumas das configurações da cultura de consumo, como somos produzidos nessa condição cultural e de que modo o Caderno, como um artefato dessa cultura, apresenta-se articulado a ela.This Dissertation aims to deconstruct discourses concerning the ways of being young in the contemporary articulated to consumption as social practice. I understand that it is through the produced strategies in the scope of the consumer culture where objects, images, desires, identities, values, ways of being can be transformed into products: they can be ‘acquired', consumed and finally discarded. They become objects to used and to shown. As an artifact between much others that circulate and are produced in this consumer culture is the section Patrola – a newspaper supplement that circulates on Fridays in Zero Hora, in the State of Rio Grande do Sul. Thus, I realize a reading (one of those that is possible to) about media, consumer culture and youths. In some way, it is a reading that the Cultural Studies, theoretic field in which I movement myself, make possible to produce. In order to this dissertation be constructed as it is, the corpus is constituted of different materials: select edition of the Patrola section, research in many sites, incursions in virtual communities of orkut and in blogs, talks with young readers of the Patrola through Messenger and e-mails with the editor of the section. In the analyses, I reflect about modes of address of Patrola section, discussing some strategies of the section that interpellate young for its pages, as well as inviting them to consume the ‘announced' products. I also analyze how the discourses of Patrola not only suggest consumption of products that can constitute ways of being young, but how they can teach what consume to own theses ways, in order to adopt desirable positions of being young. From the problematization of this study concerning to Patrola, its possible to understand how one invests in the promising young market, not only producing manufactures for them as well as transforming the manufactured products by the young into income-producing. Still, I could examine how the youth cultures become a product, once that many people desire to be young through the consumption of products that one says to belong to them. Therefore, thought the section’s pedagogy potential, I looked for understand some of the configurations of the consumer culture, how we are produced in this cultural condition and the way that Patrola, as an artifact of this culture, is articulated to it.
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Ronaritivichai, Anjaruwee 1962. "CULTURAL AND OTHER STUDIES ON FUNGI THAT DECAY ARIZONA CYPRESS." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/275483.
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McFadden, Thomas Bernard. "Influence of hormones on synthesis and secretion of milk proteins by mammary tissue from male and female cattle of beef and dairy breeds." Thesis, Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/101238.
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The ability of mammary tissue from prepubertal bulls and heifers of beef and dairy breeds to respond to hormonal stimuli through synthesis and secretion of milk proteins was studied. Experimental animals were six to eight month old Angus and Holstein cattle. All subjects were injected with estradiol and progesterone for seven days and slaughtered on day 15. Mammary tissue was explanted and cultured for 96 h in basal medium (B) which contained hormones necessary for maintenance, or stimulatory medium (P), further supplemented with prolactin. Selected cultures were incubated for 24 h in B or P medium containing 3H-amino acids. Concentrations of non-labeled alpha-lactalbumin (Alac), 3H-Alac, and 3H-total protein (TP) were determined in media and in explant homogenates.
Among cultures of bull mammary tissue, Angus explants secreted greater overall quantities of 3 H-TP and 3H-Alac than Holstein explants (p<.05). Secretion of Alac was also greater in Angus cultures at two of eight treatment periods (p<.01). Concentrations of all three protein fractions were likewise enhanced in homogenates of Angus explants for at least three of four treatment periods (p≤.05). Presence of prolactin in medium stimulated secretion of Alac (p<.005), and accumulation of all three fractions in explants (p<.10).
Holstein heifer explants secreted more Alac at three of eight treatment periods than Angus explants (p<.0005). Overall secretion of ³H-TP and ³H-Alac also was elevated in Holstein over Angus females (p<.10), as were concentrations of all three fractions in homogenates (p≤.01). Presence of prolactin had no direct effect on any protein parameters in female tissue.
I conclude that mammary tissue of immature bulls and heifers can be hormonally induced to express it's genetic merit for milk production (based on breed differences), through synthesis and secretion of milk proteins. Prolactin stimulated protein production in bulls but not in heifers. These findings indicate that similar methods of stimulating mammary tissue to produce milk proteins may be adaptable for commercial evaluation of genetic potential for milk production, especially in young bulls.M.S.
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Stewart, Paul M. "Diatom and protozoan community analysis and colonization on artificial substrates in lentic habitats." Diss., Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/49888.
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The purpose of this research was to examine the colonization process and relationship of physico-chemical parameters to diatom and protozoan communities colonizing polyurethane foam (PF) artificial substrates in lentic habitats. This was the first study to utilize multivariate techniques for comparison of protozoan and diatom communities
The following hypotheses were examined in this study:
1. diatom and protozoan species accrual is similar because the organisms are approximately the same size and share similar ecological conditions,
2. protozoan assemblages are influenced by the physicochemical parameters of their environment, and
3. diatoms and photosynthetic protozoans are more closely related to the physico-chemical parameters of their environment than are the protozoans of all trophic groups.
PF substrates were placed in the littoral zone of lentic habitats. Substrates were sampled through a time series and examined for their diatom and protozoan species' presence-absences. The first hypothesis was tested by using the MacArthur-Wilson equilibrium model and by fitting the data to the model by non·linear least squares regression. Protozoan species accrual fit the model in most cases, while diatom species accrual did not. The second part of the research dealt with five lentic habitats in northern lower Michigan which were sampled as described above and concurrent with organismal sampling several physico-chemical parameters were sampled. These environmental parameters included pH, alkalinity, conductivity, temperature, and concentrations of dissolved oxygen, chloride, silica, ammonia, and total and ortho-phosphate. Protozoan communities were examined using reciprocal averaging ordination. It was found that the bog and marsh had distinct communities, while the three lakes did not. Several physicochemical parameters and factors correlated significantly with axes generated by samples in species space. The final section tested the degree of relationship among diatoms, autotrophic protozoans, and protozoans to the physicochemical parameters and factors. pH had the highest correlations with the first axes for each group. Diatom communities had the greatest degree of relationship to the physico-chemical parameters, evidence for this is provided by the greatest number of correlations between ordination axes and the physico-chemical parameters and factors.Ph. D.
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Hajdu, Melissa Anne. "Effect of culture conditions, donor source, and injection site on in vitro development of deoxyribonucleic acid microinjected porcine zygotes." Thesis, This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-12172008-063219/.
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Watt, Diane P. "Juxtaposing Sonare and Videre Midst Curricular Spaces: Negotiating Muslim, Female Identities in the Discursive Spaces of Schooling and Visual Media Cultures." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/19973.
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Muslims have the starring role in the mass media’s curriculum on otherness, which circulates in-between local and global contexts to powerfully constitute subjectivities. This study inquires into what it is like to be a female, Muslim student in Ontario, in this post 9/11 discursive context. Seven young Muslim women share stories of their high schooling experiences and their sense of identity in interviews and focus group sessions. They also respond to images of Muslim females in the print media, offering perspectives on the intersections of visual media discourses with their lived experience. This interdisciplinary project draws from cultural studies, postcolonial feminist theory, and post-reconceptualist curriculum theorizing. Working with auto/ethno/graphy, my own subjectivity is also brought into the study to trouble researcher-as-knower and acknowledge that personal histories are implicated in larger social, cultural, and historical processes. Using bricolage, I compose a hybrid text with multiple layers of meaning by juxtapositing theory, image, and narrative, leaving spaces for the reader’s own biography to become entangled with what is emerging in the text. Issues raised include veiling obsession, Islamophobia, absences in the school curriculum, and mass media as curriculum. Muslim females navigate a complex discursive terrain and their identity negotiations are varied. These include creating Muslim spaces in their schools, wearing hijab to assert their Muslim identity, and downplaying their religious identity at school. I argue for the need to engage students and teacher candidates in complicated conversations on difference via auto/ethno/graphy, pedagogies of tension, and epistemologies of doubt. Educators and researchers might also consider the possibilities of linking visual media literacy with social justice issues.
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Cordova, Amanda. "Co-culture d'embryons bovins et de cellules épithéliales d'oviducte : un modèle in vitro pour la compréhension du dialogue embryo-maternel précoce." Thesis, Tours, 2013. http://www.theses.fr/2013TOUR4030.
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L’oviducte joue un rôle central dans le transport et la préparation des gamètes, la fécondation et le développement précoce. Un dialogue embryo-maternel s’établit pour assurer le succès du développement de l’embryon et de son transport vers le site d’implantation. L’objectif principal de ce travail était de confirmer l’existence d’un dialogue moléculaire et fonctionnel précoce grâce à la coculture d’embryons bovins produits in vitro sur des cellules épithéliales tubaires bovines (BOEC). Nous avons montré que les BOEC ont un effet important sur le développement embryonnaire précoce, particulièrement pendant les 4 premiers jours. Cet effet ce traduit par un clivage accéléré, une modulation des gènes exprimés après l’activation du génome embryonnaire, un taux plus élevé de développement au stade de blastocyste et une adaptation de l’expression génique de ces blastocystes. En retour, les embryons induisent dans les BOEC, des changements d’expression de facteurs impliqués dans la réponse à l’interféron. Une spécificité régionale des profils d’expression a également été observée dans l’oviducteThe oviduct plays a pivotal role in gametes transport and final capacitation, as well as in fertilization and early embryo development. An embryo-maternal communication takes place to ensure the successful early embryo development and transport towards its implantation site. The principal aim of this research was to confirm the existence of such early embryo-maternal molecular and functional dialogue using bovine oviduct epithelial cells (BOEC) as coculture to support the development of in vitro produced bovine embryos. We showed that BOEC had an important effect on early embryo development, especially during the first 4 days. This effect translates into accelerated cleavage kinetics, modulation of gene expression after embryonic genome activation, increased rate of embryo development to the blastocyst stage and improved gene expression profile. Moreover the embryos are triggering a BOEC response by upregulating genes related to interferon signaling. A regional specificity of gene expression profile in the oviduct has also been detected
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Klaussner, Miriam. "An examination of communication across cultures in news media and at informal/personal levels : with concentration on relations among two South East Asian countries and Australia and those two countries and Germany." Thesis, Queensland University of Technology, 2002.
Find full textAbstract:
In the age of globalisation dominated by mass communication, the flow of information contributes to a big extent to the worldviews of its "global citizens".
From this point of view the mass media can be seen as one of the most salient sources of cross-cultural communication. This study investigates mass
communication across cultures, focusing on South East Asia (Malaysia and Singapore), Australia and Germany. The centre of attention is the Western media coverage of South East Asia and vice versa. In this context a content analysis of
newspapers of the three regions has been conducted. In addition, working practices and conditions of Western foreign correspondents in South East Asia have been
examined. Apart from the investigation of inter-cultural media coverage, another focus of attention will be the examination of two levels of communication: The business
level, concentrating on issues like e.g. the Asian business etiquette; and the private level, looking into the transition to a different culture from the perspective of Australian and German expatriates. Apart from investigating mass communication across cultures and to provide a written analysis of the findings, a series of radio documentaries in English and in German has been produced. They cover the following issues: Foreign correspondents in South East Asia, the expatriate-lifestyle of Australians and Germans in South East Asia, business etiquette in Asia, student exchange Germany-Asia, image and prejudices East-West and Tourism.
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Silva, Antonio Cardoso da. "Produção de Quitina e Quitosana em cultura submersa de Rhizopus arrhizus nos meios milhocina e sintético para Mucolares." Universidade Católica de Pernambuco, 2007. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=178.
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Investigações foram realizadas com fermentação submersa de Rhizopus arrhizus para produção de biomassa e dos co-polímeros quitina e quitosana, através do cultivo em meio sintético para Mucorales e milhocina, como substrato alternativo. Neste sentido, foram realizadas fermentações em frascos de Erlenmyers de 250 mL de capacidade, contendo 50 mL dos meios, foram inoculados em duplicatas com 1% de uma suspensão de 107/esporos por mL, incubados sob agitação orbital de 150rpm. A cada 24 h foram realizados conteúdo em biomassa, consumo de glicose, além da estimação e caracterização de quitina e quitosana e o pH foi monitorado no decorrer dos estudos (96h). Os dados obtidos foram validados utilizando uma análise por regressão não linear, visando explorar o potencial e versatilidade dos mucorales na produção dos co-polímeros. Os resultados obtidos com o meio sintético para Mucorales demonstraram um aumento máximo de biomassa com 72 h de cultivo submerso. A glicose foi totalmente consumida pelo metabolismo do fungo com 96h, com pH 3,2 e conseqüente estágio de declínio celular. A produção máxima de quitina e de quitosana por R. arrhizus foi de 73,5 mg e 158 mg, respectivamente, por grama de biomassa em 48 h de cultivo, com velocidade máxima de crescimento de Max 0,036(h-1)e tempo de geração de 4,6 h. Por outro lado, o cultivo submerso de R. arrhizus em milhocina, nas concentrações de 4,8 e 16%, como meio alternativo e de baixo custo, demonstrou crescimento máximo de 16,8 g/L, na concentração de 8% de milhocina, observando-se Max 0,064(h-1. Altos rendimentos de quitina (575mg/g de biomassa) e quitosana (416 mg/g de biomassa) foram obtidos com milhocina a 8%, com 72 h de cultivo, respectivamente, e pH variando de 6,5 para 8,2. Todos os copolímeros isolados foram caracterizados pelo índice de cristalinidade e espectro de absorção ao raio infravermelho, confirmando um alto grau de pureza quando comparados aos padrões de quitina e quitosana. Os dados obtidos experimentalmente de produção de quitina e quitosana foram validados pela estimativa de regressão não linear, demonstrando um bom ajuste das equações e reprodutibilidade. Os resultados com a fermentação submersa de R. arrhizus comparando milhocina a 8% com o meio sintético para Mucorales observou-se um aumento considerável de 782% e 263%, respectivamente, para a produção de quitina e quitosana. Assim, os resultados obtidos sugerem R. arrhizus como fonte de produção dos co-polímeros, como também a milhocina, considerando o potencial nutritivo e o baixo custoInquiries had been carried out with submerged fermentation of Rhizopus arrhizus for production of biomass and copolymers chitin and chitosan, using the culture in synthetic medium for Mucoralean and corn steep liquor, as alternative substratum. In this direction, fermentations in Erlenmyers flasks of 250mL had been carried out, contend 50 mL of the media had been inoculated in duplicates with 1% of a suspension of 107/spores/mL, incubated under orbital shaker of 150rpm. To each 24 h had been carried out the content in biomass, glucose consumption, production and characterization of chitin and chitosan, and pH was monitored in elapsing of the studies (96h). The dates had been validated using an analysis for not linear regression, aiming at to explore the potential and versatility of Mucoralean in the production of copolymers. The results obtained with the synthetic medium for Mucoralean had demonstrated a maximum increase of biomass at 72 h of submerged culture. The total of glucose total was consumed by the metabolism of fungus at 96h, with pH 3,2 and consequence period of behavior of cellular decline. The maximum production of chitin and chitosan was 73.5mg and 158 mg, respectively, for gram of biomass with 48 h of cultivation, and maximum speed of growth of Max 0.036 (h-1) and generation time of 4.6h. On the other hand, the submerged culture of R. arrhizus in corn steep liquor, concentrations of 4, 8 and 16%, as alternative medium and of low cost showed maximum growth of 16.8 g/L, in the concentration of 8% of corn steep liquor, observing a Max 0.064h-1. High yields of chitin (575 mg/g biomass) and chitosan (416mg/g biomass) could be achieved using the medium containing corn steep liquor at 8%, with 72 h of cultivation, respectively, and pH varying of 6.5 to 8.2. All the isolated copolym rs in both culture media were characterized by index of crystallinity and absorption to the infra-red ray peaks, and were confirmed using the chitin and chitosan standards. The experimental data obtained with chitin and chitosan were validated by the estimation of not linear regression, demonstrating to a good adjustment of the equations and reproducibility. The results with the submerged fermentation of R. arrhizus were compared corn steep liquor at 8% with synthetic medium for Mucoralean fungi, and was observed an increase of 782% and 263% respectively, for chitin and chitosan production. The results obtained suggest R. arrhizus as source of production of the copolymers and as well as the corn steep liquor, considering the nutritional potential and the low cost
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Van, Ginkel Marney. "Molecular characterisation of the multi-antibiotic resistant bacteria, Klebsiella Pneumoniae isolated from nosocomial infections." Thesis, Cape Peninsula University of Technology, 2017. http://hdl.handle.net/20.500.11838/2735.
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Thesis (MSc (Biomedical Technology))--Cape Peninsula University of Technology, 2017.Background: It is well established that Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogenic organism that has been frequently identified as the cause of nosocomial and community acquired infections. Furthermore, studies have shown that over the last few decades strains of the genus Klebsiella have systematically developed resistance to numerous antibiotics. Aims and Methods: The primary aim of this study was to investigate the prevalence of K. pneumoniae in nosocomial and community isolates in the Western Cape province of South Africa. Various identification techniques such as the polymerase chain reaction (PCR) using the API 20 E, the VITEK®2 system, primers specific for the 16S-23S rDNA ITS region and the Matrix-assisted laser desorption/ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) were compared for the identification of this pathogen. The VITEK 2 system was used to detect antibiotic resistant profiles of the K. pneumoniae isolates and to identify the extended spectrum beta-lactamase (ESBL) phenotypic among these isolates. The PCR was used to detect Beta-lactam genes viz. CTX-M (blaCTX-M), TEM (blaTEM) and SHV (blaSHV) respectively in both the genome and plasmid DNA of K. pneumoniae using gene specific primers. Results: In total 57 agar plate bacterial cultures or glycerol stock bacterial cultures were obtained during 2011. Of the 57 isolates, the API 20 E test identified 47 (82.5%) of the isolates (n = 57) as K. pneumoniae while 10 isolates (17.5%) were identified as Raoultella species. The VITEK 2 method and PCR identified all 57 isolates as K. pneumoniae (100%). Of the isolates, 82.5% (47/57) were positively identified as Klebsiella species, 14% (8/57) were identified as Klebsiella variicola and 3.5% (2/57) were shown as no reliable identification (NRI) when using the MALDI-TOF MS. Examination of the 57 isolates using primers specific for the CTX-M (blaCTX-M), TEM (blaTEM) and SHV (blaSHV) respectively showed the following: PCR amplicons for the TEM gene were produced successfully for 46 (81%) of the 57 isolates included in this project, while 11 (19%) of the samples did not yield any TEM amplicons; PCR amplicons for the blaSHV gene were obtained successfully for 56 (98%) of the 57 DNA samples, while 1 sample (2%) did not yield any SHV amplicons; and PCR amplicons for the blaCTX-M gene were produced successfully by 89% (n = 51) of the DNA samples included in this project, while 11% (n = 6) did not yield any CTX-M amplicon. Extended-spectrum beta-lactamase phenotypes had been confirmed in 84% (n = 48) K. pneumoniae isolates while nine isolates were found to be non-ESBL. Resistance rates for these 48 isolates were high and showed resistance patterns of: Amoxicillin/Ampicillin, Amoxycillin/Clavulanate, Ceftriaxone/Cefotaxime, Cefuroxime/Cefprozil and Ceftazidime (100%, n = 48); Piperacillin/Tazobactam and Cefoxitin (98%, 47/48); Cefepime (96%, 46/48); Aztreonam (94%; 45/48); Tobramycin (81%, 39/48); Gentamycin and Ciprofloxacin (77%, 37/48); Trimethoprim/Sulfamethoxazole (67%, 32/48); and Tigecycline (25% 12/48). Conclusion: For the analysis by all four methods employed, a total agreement of 68.4% was obtained, indicating the positive identification of K. pneumoniae in 39 of the 57 samples analysed. An average agreement of 28.1% was then obtained for the comparison of results generated for three of the methods utilised, while a 3.5% average agreement was obtained for at least two methods. Furthermore, all four methods agreed that 82.5% of the isolates were Klebsiella species while three methods agreed that 17.5% of the isolates were Klebsiella species. Based on the results obtained in the current study, PCR and VITEK 2 were the methods of choice for the identification of K. pneumoniae. The current study also showed, that ESBL-K. pneumoniae strains are present in the Western Cape province, South Africa; with high resistance profiles to numerous antibiotics including the Cephalosporins.
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Sólorzano, Cháves Eddyn Gabriel. "Avaliação de cultivos de Kappaphycus alvarezii em diferentes condições climáticas e hidrólise enzimática do resíduo gerado pós-processamento de Carragena /." Araraquara, 2017. http://hdl.handle.net/11449/152614.
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Orientador: Fernando MasarinBanca: Banca: Kelly Johana Dussan Medina
Banca: Samuel Conceição de Oliveira
Resumo: Kappaphycus alvarezii é uma macroalga vermelha utilizada principalmente para produção industrial de hidrocolóides (principalmente carragena). O cultivo de K. alvarezii foi estabelecido como uma atividade econômica importante em mais de 20 países. Os principais componentes da biomassa de K. alvarezii são carboidratos. O processamento de carragena a partir de K. alvarezii promove a formação de um resíduo rico em glucana. Objetivo: Avaliar o desenvolvimento de cultivos de K. alvarezii em diferentes condições climáticas e averiguar o efeito da hidrólise enzimática sobre os resíduos obtidos pós-processamento de carragena. Métodos: A parte experimental envolveu o cultivo de 4 cepas de K. alvarezii ao longo de um ano no oceano atlântico em Ubatuba-SP. A partir dos dados dos cultivos foram calculadas as velocidades de crescimento e produtividades. Cultivos selecionados com base nos dados de produtividade foram previamente misturados e homogeneizados e submetidos a um pré-tratamento com KOH 6%. As biomassas previamente pré-tratadas com KOH 6% foram extraídas com água para a obtenção das carragenas e dos resíduos. As frações obtidas no processamento foram caracterizadas quimicamente. Os resíduos foram hidrolisados enzimaticamente com celulases comerciais. Resultados: Foram diferenciados três níveis de produtividade (A, setembro-dezembro de 2013; B, janeiro-março de 2014; C, abril-junho 2014). Os valores de produtividade foram maiores no nível B e intermediários nos níveis A e C. Os val... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The fungus of genus Paracoccidioides is the etiological agent of paracoccidioidomycosis (PCM), an important mycosis in Brazil and Latin America. There are different available therapies, as antifungals belong to the class of polyenes, azoles and sulfonamides. The development of new antifungal agents with greater effectiveness is required to prevent relapse and reduce side effects, the period of treatment and sequelae. The gallic acid molecule with different modifications (alkyl gallates) showed antifungal activity against a representative panel of pathogenic fungi. The decyl gallate (G14) was the study object of this work, for which the Paracoccidioides-host interaction was connected with the mechanism of action. Therefore, the fungus Paracoccidioides lutzii (Pl01) was challenged with the G14 to analyze the changes in the development of budding and cell viability by MTT assay. Then, it was assessed the influence of the G14 in the interaction rate of Pl01 with murine alveolar macrophage lineage AMJ2-C11, with mammalian lung lineage A549 and MRC-5, and hemocytes of Galleria mellonella larvae using assays by flow cytometry. The compound G14 was evaluated for survival rate of infected G. mellonella larvae and to capacity of modulation of nitric oxide production in RAW macrophages. The mechanism of action was performed by chemical-genetic interaction assay followed by genetic interaction, which involves comparing a mutant collection, of Saccharomyces cerevisiae yeast, with hypersensitive profile to G14 to double mutant database to predict via affected. In P. lutzii (Pl01)... (Complete abstract click electronic access below)
Mestre
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Gillmann, Antoine. "Étude de la survie de contaminants bactériens modèles d’origine industrielle, isolés d’environnements oligotrophes, et élaboration de milieux synthétiques permettant leur croissance." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAJ004/document.
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La mise au point de milieux de culture permettant de mettre en évidence rapidement et de manière reproductible des micro-organismes exigeants représenterait une évolution significative dans le contrôle des produits et procédés industriels. Deux milieux de culture synthétiques ont été élaborés pour répondre à ce besoin. Le développement des milieux de culture a été réalisé en combinant l’analyse de composés nutritionnels à l’étude de certains métabolismes bactériens. Les formulations des milieux de culture obtenues permettent ainsi la croissance de micro-organismes aux exigences nutritionnelles très variées comme ceux fréquemment isolés dans l’eau industrielle. En parallèle, des expériences sur des biofilms multi-espèces, utilisant des bactéries isolées de systèmes d’eau industrielle, ont permis d’observer que la survie des bactéries en milieu pauvre en nutriments, est dépendante d’interactions coopératives, basées sur le « swarming » et le « hitchhiking »The development of culture media to quickly and reproducibly detect fastidious microorganisms represents a significant change in the control of industrial products and processes.Two synthetic culture media have been developed to reach specifications. The development was performed by combining the analysis of nutritional compounds to the study of specific bacterial metabolisms. The formulations of the resulting culture media allow the growth of microorganisms with different nutritional requirements as those frequently isolated in industrial water.In parallel experiments on multi-species biofilms, using bacteria isolated from industrial water systems, it was observed that the survival of bacteria in nutrient-poor environments is dependent on cooperative interactions, based on "swarming" and "hitchhiking"
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Enomoto, Rene. "Webbdesign i Japan och Sverige : En tvärkulturell analys av webbplatser från hög-kontextuella kulturer och låg-kontextuella kulturer." Thesis, Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-34632.
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Den här uppsatsen syftar till att analysera och identifiera skillnader och likheter i webbdesign mellan två länder, Japan och Sverige. De japanska och svenska webbplatserna för två företag etablerade i både Japan och Sverige, IKEA och DAIKIN, har analyserats utifrån en modell framtagen av Würtz (2006) i en tidigare studie på området. Denna modell är i sin tur baserad på Halls och Hofstedes teorier om kulturella skillnader och skillnader mellan så kallade låg-kontextuella kulturer (t.ex. Sverige) och hög-kontextuella kulturer (t.ex. Japan). Modellen fokuserar på användandet av animation, främjande av kulturella värderingar som maskulinitet/feminitet och individualism/kollektivism, om individer avbildas tillsammans med produkter eller inte, grad av transparens på webbplatserna och slutligen linjär kontra parallell navigation på webbplatserna. Förutom studien av webbplatserna har två intervjuer hållits med en designer/utvecklare från respektive företag för att få ökad förståelse för hur företagen tänker kring webbdesign. Studien har inte funnit några väsentliga skillnader i användandet av animation, graden av transparens eller navigation mellan de studerade webbplatserna men skillnader i kulturella värderingar har identifierats. De japanska webbplatserna främjar familjeorienterade värderingar och stereotypiska könsroller i valet av bilder på webbplatserna. De svenska webbplatserna främjar värden som är mer typiska för låg-kontextuella kulturer som till exempel livsstil och frihet. Det har också noterats att det japanska företaget använde sig av en maskot vilket kan tolkas som typisk för japansk kultur. I intervjuerna gav utvecklarna från båda företag liknande svar kring hur de tänker om användande av bilder och animation kontra text på sidorna. Den japanska utvecklaren förklarade att japanska kunder uppskattar kundrecensioner för att minska graden av osäkerhet vilket kan sägas vara typiskt för Japan. Den sammanfattande slutsatsen från studien är att kulturella skillnader i webdesign minskar, kanske på grund av globaliseringen av företag och samhällen.This thesis aims to analyze and capture differences and similarities in webbdesign between two countries, Japan and Sweden. The Swedish and Japanese websites of two companies established in both Japan and Sweden, IKEA and DAIKIN, have been analyzed based on a model devised by Würtz (2006) in a previous study of the same topic. This model is in turn based on Hall and Hofstede’s theories on cultural differences and differences between so-called low-context cultures (e.g. Sweden) and high-context cultures (e.g. Japan). The model focuses on the use of animation, promotion of cultural values such as masculinity/femininity and individualism/collectivism, individuals being depicted together with products or not, level of transparency in the web design and finally linear vs. parallel navigation on the websites. Besides the study of the websites two interviews were held with a designer/developer from each company to get further input into how the companies think about their web design. The study did not find any significant differences in the use of animation, transparency and navigation between the websites studied but differences in cultural values could be seen in terms of the Japanese sites promoting more family-oriented values and stereotypical gender roles through the pictures used on the websites. The Swedish sites promoted values more typical for low-context cultures such as lifestyle and freedom. It was also noted that the Japanese company featured a mascot which can be said is typical for Japanese culture. Regarding the interviews the developers had similar thoughts on the use of text versus pictures and animations. The Japanese developer explained that Japanese customers appreciate customer reviews in order to lower uncertainty which can be said is typical for Japan. The overall conclusion from this study however is that cultural differences in web design are becoming less, perhaps due to the globalization of business and society.
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Adeleke, Rasheed Adegbola. "Isolation, propagation and rapid molecular detection of the Kalahari truffle, a mycorrhizal fungus occurring in South Africa." Thesis, Rhodes University, 2007. http://hdl.handle.net/10962/d1002951.
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Terfezia pfeilii is an edible mycorrhizal fungus that thrives in the Kalahari Desert of southern Africa. It is best known by desert dwellers for its flavour and as a source of nutrition. Although the genus Terfezia is generally regarded as being an ectomycorrhizal mycobiont, the exact mycorrhizal type formed by T. pfeilli and its' associated host plants remains uncertain. Discovery of the host plants for T. pfeilii would first be required in order to further investigate the life cycle and cultivation of this truffle. This study focussed on the isolation of mycelia from the ascocarp, optimising the growth conditions of the mycelial cultures, rapid molecular identification of T. pfeilii, investigation of potential helper bacteria and mycorrhizal synthesis experiments. T. pfeilii ascocarps were harvested from the Spitskop Nature Reserve in Upington, South Africa. Ascocarps were successfully identified using both morphological and molecular methods. Despite the delayed growth mostly caused by contaminating microorganisms, the isolation of T. pfeilii mycelia culture was successful. Molecular techniques were used to confirm the identity of the pure culture. Further studies were conducted on ways to improve the growth conditions of the mycelial culture on Fontana medium. An optimum temperature of 32°C, the addition of Bovine Serum Albumin as a nitrogen source and a pH of 7.5 significantly improved the growth of T. pfeilii in vitro. A rapid PeR-based molecular method was developed to speed up the identification of T. pfeilii. Specific primers that can exclusively amplify the ITS region of T. pfeilii were designed and used to identify both the ascocarps and the mycelial culture. The specificity of these primers was confirmed by their inability to amplify DNA from the isolates of contamining fungi obtained during the isolation process. Molecular comparison was made to confirm the reclassification of South African samples of T. pfeilii as Kalaharituber pfeilii as proposed by Ferdman et al.,(2005). However, in this study, the name T. pfeilii has been retained. A total of 17 bacterial isolates were obtained from the fruiting bodies of T. pfeaii and these were tested for stimulation of mycelial growth in vitro, indole production and phosphate solubilising capabilities. Bacterial isolates that showed potential to be Mycorrhization Helper Bacteria (MHB) were identified as Paenibacillus sp., Bacillus sp. and Rhizobium tropici. Selected plant seedlings were inoculated with T. pfeilii cultures or ascocarp slurry in order to re-establish the mycorrhizal association. After 8 months, light microscopy observations revealed an endomycorrhizal type association between Cynodon dactylon and T. pfeilii. This was confirmed with molecular analysis using specific T. pfeilii ITS primers. After 15 months, molecular methods confirmed Acacia erioloba as another host plant. These results have provided essential information paving the way for further investigation into the life cycle and biology of the Kalahari truffle.KMBT_363
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Alves, Maria Rosângela Calheiros. "Produção de quitosana por Mucor subtilíssimus por fermetação semi-sólida em meio alternativo e aplicação na remoção do corante azul de metileno." Universidade Católica de Pernambuco, 2013. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=1208.
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Conselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Quitosana é um polímero natural derivado da desacetilação da quitina, oriundo da parede celular de fungos e exoesqueletos de crustáceos. Devida a sua estrutura química a quitosana apresenta propriedades de grande importância biotecnológica com diversas aplicações nas áreas ambientais, agricultura, cosméticos entre outras. Para averiguar a produção de quitosana por micro-organismo, estudos foram realizados utilizando o fungo Mucor subtilíssimus UCP/WFCC 1262 isolado do solo da caatinga do estado de Pernambuco, através do planejamento fatorial completo de 23, por fermentação semisólida (FSS), utilizando batata doce (Ipomoea batatas L.) suplementada com milhocina (resíduo industrial) e extrato de levedura, sendo as variáveis respostas produção de biomassa e quitosana. Para a produção de biomassa o ensaio 5 cuja composição: 3g de batata doce, 8ml de milhocina, e não utilizando extrato de levedura apresentou o melhor resultado com 13,32g/L de biomassa, e para quitosana o ponto central com 120,96 g/100g de biomassa com seguinte composição: 20g de batata doce, 6ml de milhocina, 0,1ml de extrato de levedura. A caracterização da quitosana demonstrou um grau de desacetilação de 60%. A quitosana microbiológica obtida foi testada quanto a sua capacidade ambiental no processo de descoloração do corante catiônicao, azul de metileno (AM), empregado na indústria têxtil tendo como variáveis pH, tempo e temperatura . Os resultados obtidos sobre eficiências de descoloração do azul de metileno pela adsorção da quitosana demonstraram que o pH 6 foi mais eficiente na descoloração do AM com a biossorção de 92,73%, na condição 8,30mg do adsorvente para 20 mg de AM em solução aquosa, sugerindo seu emprego em processos de biorremediação de efluentes têxteis.
Chitosan is a natural polymer derived from deacetylation of chitin, derived from the cell wall of fungi and exoskeletons of crustaceans. Due to its chemical structure exhibits properties of chitosan great biotechnological importance with many applications in the environmental fields, agriculture, cosmetics, among others. To check chitosan production by micro-organism, studies were performed using the fungus Mucor subtilíssimus UCP / WFCC 1262 isolated from soil of the savanna of the state of Pernambuco, through full factorial design of 23, by solid state fermentation (FSS) using sweet potato (Ipomoea batatas L.) supplemented with corn steep liquor (industrial waste) and yeast extract, and the response variables biomass and chitosan. For biomass production testing 5 whose composition 3g sweet potato, 8ml milhocina, and not using yeast extract showed the best result with 13.32 g / L of biomass, chitosan and the center point with 120.96 g / 100g biomass with the following composition: 20 g sweet potatoes, 6ml of corn steep liquor, 0.1 ml of yeast extract. The characterization of chitosan showed a degree of deacetylation of 60%. Microbiological chitosan obtained was tested for its environmental capacity in the discoloration of catiônicao dye, methylene blue (MB), process used in the textile industry having variables such as pH, time and temperature. Results obtained with efficiencies discoloration of methylene blue adsorption of the chitosan showed that the pH 6 was more efficient in bleaching AM biosorption with 92.73% under the condition of 8.30 mg to 20 mg of adsorbent in solution PM aqueous, suggesting its use in bioremediation processes of textile effluents.
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Peng, Hsin-Pey. "The rise of regionalisation in the East Asian television industry: a case study of trendy drama 2000-2012." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2012. https://ro.ecu.edu.au/theses/534.
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This thesis examines the contemporary Taiwanese television industry and its influence on the Asian TV market and popular culture in Asia. It explores the East Asian TV industry’s ability to produce a specific regional TV genre – that of trendy drama – as a means of representing the tastes and lifestyles of a new audience. I claim in the thesis that the East Asian TV industries have produced trendy drama for an emerging middle class audience in Asia. Trendy drama still is one of the most popular genres at the level of local TV productions; it can also be sold to an Asian regional audience. The main premise of the study is that the media has the symbolic power to centralise most social resources and technology, and because of that they can produce certain cultural meanings influential to ordinary people’s social and cultural experience. A study of the rise of regionalisation which specifically focused on the East Asian TV industry, has led to this case study of trendy drama. In the case study I analyse how East Asian TV industries produce and sell these types of local TV productions to a wider TV market. After the review of regionalisation literature, the study examines the specific content of the TV genre, trendy drama, within the context of the Asian TV market. This raises questions about the role of trendy drama and its function in the rise of regionalisation from political and economic perspectives. The answers to these questions are then used to examine the production of Taiwanese idol drama through a filmic and semiotic analysis. The earlier findings are supported by the television producers’ and directors’ (professionals’) practical insights into why and how they produce trendy drama for the Asian market. Macro- and micro-level approaches used in this study demonstrate the transition from a global television industry dominated by America to the way East Asian TV industries earlier on drew from the American TV industry’s values, technical knowledge and resources. However, ultimately the East Asian TV industry developed their own expertise which is why they now have the symbolic power to sell to audiences within the region. Furthermore, East Asian TV industries today have the ability to centralise enormous resources so they can produce culturally shared meanings, which is becoming part of popular culture in Asia. Consequently, the media’s symbolic power enhances the rise of regionalisation in East Asian TV industries. It is intended that this project will inform further debate about the changing configuration of television markets within the Asian region and the role of the media in mediating popular culture within the contemporary media age.
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Ferjoux, Céline. "Discours de l'innovation et médiations de la programmation. La télévision à l'heure du numérique." Thesis, Paris 2, 2011. http://www.theses.fr/2011PA020053.
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Ce travail de recherche doctoral en sciences de l'information et de la communication propose d'appréhender un média, la télévision, en France, au printemps 2005. Cette période correspond à un épisode de mutation qui voit l'ancienne télévision analogique migrer vers un environnement numérique et informatique. Notre propos cherche à explorer la notion de média en articulant trois dimensions : le contexte, le discours et le format. Il s'agit d'explorer des mécanismes qui configurent les pratiques médiatiques, au printemps 2005, à travers : la définition d'un contexte d'innovation technique, l'analyse de discours d'actualité qui affirment des lignes éditoriales et enfin, la valorisation de la programmation et la conception de formats de programmes qui intègrent la culture numérique. Les objets construits au cours de cette recherche sont des formes médiatiques, composées de codes identifiables et marquées par le contexte culturel de leur production. En tant que pratique médiatique, la programmation télévisuelle est transversale. Elle assure une continuité entre les trois dimensions : le contexte dans lequel elle est agencée, les discours qui la prennent pour objet, enfin, les codes et formats télévisuels qu'elle intègre. Notre projet consiste à étudier la programmation télévisuelle comme un objet médiatique complexe, configuré par les enjeux techniques, industriels et économiques liés à la diffusion d'une innovation. Ainsi, dans une première partie, les modalités de la médiatisation des choix de programmation seront appréhendées en observant les discours d'actualité produits et diffusés le 31 mars 2005, à l'occasion du lancement en France de la télévision numérique terrestre. La nature éditoriale du média télévision, et la programmation comme objet de discours d'autopromotion seront mises en évidence. Puis, dans une deuxième partie, les nouvelles formes de la médiation de la programmation seront examinées à travers un corpus web, composé des pages des sites des chaînes de télévision pré-numériques"Discurses on innovation and broadcast programming mediations. Television in the digital age". This doctoral research in information and communication sciences (Media Studies) offers to understand the functional behaviour of a media, the television, in France, during the spring 2005. This period corresponds to an episode of change that sees the old analog TV migrate to a digital environment. Our work seeks to explore the notion of media articulating three dimensions : context, discourse and format. This is to explore mechanisms that shape media practices in the spring of 2005, through : the definition of a technical innovation context, the analysis of the news discourse which affirms editorial strategies and finally, the development of broadcast programming and programs designs that incorporate the digital culture. The objects built in this research are media forms, composed of identifiable codes and marked by the cultural context of their production. As a media practice, television programming is transversal. It ensures continuity between the three dimensions: the context in which it is arranged, the speeches which are intended, finally, the television codes and formats that are integrated. Our project is to study broadcast programming as a complex media object, configured by the technical, industrial and economic issues related to the diffusion of an innovation. Thus, in a first part, the terms of the media coverage of the scheduling choices will be understood by observing the news discourses produced and distributed on March the 31st 2005, at the launching in France of the digital terrestrial television. The editorial nature of television, and the broadcast programming as an object of discourse of self-promotion will be highlighted. Then, in a second part, the new mediation forms of scheduling will be examined through a web corpus, consisting of pre-digital broadcasters web pages
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Robinson, Amanda L. "Detection and enumeration of sublethally-injured Escherichia coli O157:H7 using selective agar overlays." Muncie, Ind. : Ball State University, 2009. http://cardinalscholar.bsu.edu/672.
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Lopes, Isabella de Cenço [UNESP]. "Produção de conídios do fungo entomopatogênico Metarhizium anisopliae em diferentes condições de cultivo e em biorreator de bandeja." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/139445.
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O objetivo deste trabalho foi de aumentar a produção de conídios de Metarhizium anisopliae através de alterações do substrato e das condições de cultivo e produzi-lo em biorreator de bandeja. Os substratos testados foram arroz tipo 1, quirera de arroz e farelo de arroz, sendo o cultivo realizado em embalagens plásticas contendo 10 g de substrato seco. Inicialmente, foi empregado arroz tipo 1 como substrato, variando-se as formas de umidificação no seu preparo, sendo o cultivo realizado em embalagens plásticas contendo 10g de substrato seco. Determinada a condição adequada de umidificação, os substratos arroz tipo 1 e quirera de arroz foram submetidos a diferentes condições de fotoperíodo: escuto contínuo, claro contínuo e escuro e claro alternados. O farelo de arroz foi adicionado a bagaço de cana-de-açúcar de modo a estruturar fisicamente o meio de cultivo. Os melhores resultados foram obtidos com arroz e quirera de arroz. A primeira alternativa de ampliação de escala foi realizada em embalagens plásticas, utilizando arroz tipo 1 e quirera com 500g de substrato seco. A etapa seguinte da ampliação de escala foi em um biorreator de bandeja com aeração realizada sobre a camada de substrato, sendo os testes realizados com arroz tipo 1, e duas espessuras de camada partículas, 2 e 4 cm. Em todos os ensaios, a resposta observada foi a concentração final de conídios. Foi testada ainda a virulência dos conídios produzidos em relação a lagartas de Diatraea flavipennella nas diferentes condições de produção. De acordo com os resultados apresentados, o farelo de arroz não é uma boa opção de substrato devido a sua pouca praticidade de manipulação e a quirera apresentou resultados satisfatórios nos ensaios, podendo ser considerada uma opção viável para utilização industrial devido ao seu baixo custo. O biorreator de bandeja elaborado apresentou bons resultados de produção de conídios em relação a produção em embalagem plástica de maior capacidade com o substrato arroz tipo 1, a qual, no entanto, apresentou produção de esporos inferior à da embalagem de menor capacidade. Os testes de virulência comprovaram a eficiência dos conídios em todos os ensaios com pequenas variações no tempo de mortalidade.
The work targeted the increase of the production of spores of Metarhizium anisopliae through modifications of the substrate and of the cultivation conditions, and produce such spores in a tray bioreactor. Type 1 rice, broken rice and rice bran were tested as substrate, which were cultivated in plastic bags containing 10 g of dry substrate. Alternatives of humidification were tested with type 1 rice. For the best alternative of humidification, type 1 rice and broken rice were submitted to alternatives of different exposures to light, provided by a fluorescent lamp: continuous dark, continuous light, alternation between dark and light. To the bran was added sugar cane bagasse in order to provide physical structure to the culture medium. The best results were obtained with rice and broken rice and the illumination regime does not influence on the spore production. The first attempt of scaling-up the spore production was the use of plastic bags containing 500 g of dry substrate, either rice or broken rice. The next scaling-up step was in a tray bioreactor aerated flowing air parallel to the top of the cultivation medium, using type 1 rice as substrate in two different thickness of medium, 2 and 4 cm. In every experiments, the response variable was the conidia concentration. The virulence against Diatraea flavipennella caterpillars was also tested for spores produced in the different experimental conditions. The results showed that rice bran is not an efficient alternative due to its difficult manipulation, while broken rice produce conidia concentration similar to type 1 rice and can be considered a cheap alternative for industrial production of spores. The tray bioreactor presented similar results to the large capacity plastic bags, which presented lower conidia concentrations than the small capacity container. The virulence experiments showed high efficiency of the conidia in all tested samples, with little variation in the time of lethality.
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Oussi, Christian. "Pouvoirs et médias de masse : éthiques politiques et économiques." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM1007.
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Ce travail s'intéresse aux influences réciproques entre pouvoirs et médias de masse en Occident comme en Orient en insistant sur la situation particulière du Liban où les pratiques culturelles sont plus larges que dans bien d'autres pays. Notre but sera de montrer les interférences entre ces deux pouvoirs. Ce sujet n'est peut-être pas tout à fait neuf mais nous pensons y apporter des considérations nouvelles, pour ce qui est des particularités des pouvoirs dans les pays du Moyen Orient et la perception que les orientaux peuvent avoir des pouvoirs et des médias de masse dans les pays occidentaux. On s’y intéresse surtout aux pouvoirs politiques et économiques. Pour les médias de masse, on se limite aux journaux, radio, télévisions et internet. La conclusion insiste sur une nécessaire moralisation tant des médias que des hommes de pouvoir. En l’absence de prises de conscience forte, la seule solution est de recourir soit à l’exil soit à la loi à condition qu’elle soit assortie de contrôles et de sanctions. Le problème est que les Nouvelles Technologies apparaissent comme étant devenues d’une puissance incontournable et immaîtrisable à court terme. . Nul ne peut prévoir ce qui pourra en advenir. . Pour débuter on présente 4 auteurs qui ont illustré ce sujet dans la littérature et le cinéma montrant l’importance de cette interpénétration entres pouvoir et média de masse. On présente ensuite un aperçu historique sur l'évolution des pouvoirs montrant comment l'émergence des techniques d’information et de communications ont pu affecter ces différents pouvoirs. On montrera comment tout cela a pu affecter les concepts de liberté. A coté des pouvoirs politiques, un chapitre est consacré à la diffusion des informations dans le cadre des entreprises. Enfin dans une partie plus expérimentale, nous reportons des entretiens entre journalistes sur ce sujet puis dressons un cadre d’étude pour traduire les opinions de journalistes du Moyen Orient face à la question de ces influences réciproquesThis thesis aims at defining the reciprocal unfluences‘berween mass medias and political and economical powers. The research concentrates on Arabs and Middle East Countries. To start we present 4 authors that have illustrated this in the litterature and 7th art. After that we put our attention to the evolution of different powers and communication tools in past history. Then we put our attention on the different constitutions defining the limit of political powers.But also to the mangement of mass information required in the framework of firms and various institutions showing how the manner things that are presented can influence the public, executives and shareholders. In an experimental part we report interwiews taken from a pannel of journalists. Also we show the frame of an inquiry devoted to business and political people as to their attitufe vs mass medias and power influences that they can have experienced
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Imatoukene, Nabila. "Optimisation de la production d’acides gras inhabituels chez la levure Yarrowia lipolytica par fermentation de substrats bio-sourcés." Thesis, Compiègne, 2017. http://www.theses.fr/2017COMP2362.
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Les acides linoléiques conjugués (CLA) et les acides gras cyclopropanes (CFA) sont deux types d’acides gras à haute valeur ajoutée. Ils présentent un grand intérêt dans divers domaines agroalimentaire ou industriel. Les CLA sont connus pour leurs effets bénéfiques pour la santé, alors que les CFA constituent une bonne source pour la fabrication de lubrifiants, plastiques…après leur hydrogénation. Les CLA sont synthétisés par voie chimique qui génère différents types d’isomères. Les CFA sont produits par les plantes dépendantes du climat et les bactéries présentant naturellement de faibles productivités. Il semble donc intéressant de chercher des alternatives pour leurs productions. Y. lipolytica constitue une bonne alternative. Ceci passe par des modifications génétiques pour permettre ces synthèses et l’étude de l’influence des conditions de culture. Pour cela, les voies de synthèse de ces deux acides gras ainsi que les éléments qui peuvent influencer leurs productions sont à acquérir. Dans ce contexte, nous avons testé plusieurs souches possédant différentes modifications génétiques en erlenmeyer et en fermenteur sur le milieu de néo-synthèse et de bioconversion. Une souche a été sélectionnée permettant la diminution de la dégradation des CLA par le blocage de la voie de la β-oxydation. La meilleure production de CLA en fermenteur a été obtenue en présence d’huile de soja et grâce à la surexpression du gène FAD2 (302 mg. L-1). L’influence de la composition et des paramètres de culture a été étudiée. Les résultats ont montré une meilleure assimilation de la peptone de Soja Dynamis riche en acides aminés libres par la levure qui conduit à une productivité améliorée. Ceci par son effet positif sur la croissance et les productions. De plus, la stratégie Fed-batch en fermenteur a permis la production optimale de CLA (0,4 g.L-1), la limitation en azote et phosphore semble affecter leur accumulation. D’autres souches dites de premières générations productrices de CFA grâce à l’expression du gène de la CFAs d’E.coli ont été testées sur différents milieux de culture. Deux souches (GY1005 et GY1070) ont été retenues pour leur production maximale d’huile et/ou de CFA sur le milieu de néo-synthèse en fermenteur. Ceci grâce à la surexpression des gènes DGA2 et GPD1 permettant une forte accumulation de lipides et l’absence de remobilisation et de dégradation des acides gras. Les paramètres et la composition du milieu de culture tels qu’une forte agitation (1 000 rpm) et une stratégie Fed-batch en utilisant une solution de même composition que le milieu de départ ont permis de maximiser la production de CFA (1,2 g.L-1 pour GY1070 et 0,9 g.L-1 pour GY1005). Une souche dite de deuxième génération (JMY5578) a été optimisée génétiquement (expression du gène de la CFAs sous le contrôle d’un promoteur plus fort et l’expression du gène LRO1). Cette souche a été testée en erlenmeyer et en fermenteur dans le but d’évaluer l’influence des composés du milieu de culture sur la production de CFA. Les résultats ont montré un gain de 30% de CFA et 6% de biomasse en présence des chlorures de magnésium et des vitamines dans le milieu de culture. De plus, un ajout en continu de dextrose et un milieu de départ riche en azote et phosphore a permis une production de 3 g.L-1 de CFA. Nous avons étudié l’impact des promoteurs sur l’expression du gène de la CFAs dans des souches de 2éme génération. L’expression du gène sous le contrôle du promoteur php8d a donné le meilleur résultat en termes d’accumulation des CFA dans la souche JMY6068 (46% dans les lipides totaux) ; il semble être le promoteur le plus fort en comparaison avec TEFConjugated linoleic acids (CLA) and cyclopropane fatty acids (CFA) are two high value added products. They are of a great interest in various agri-food or industrial fields. CLAs are known for their health benefits, while CFAs, after their hydrogenation are a good source for the manufacture of lubricants, plastics.... CLAs are synthesized chemically which generates different types of isomers. CFAs are produced by climate-dependent plants and by bacteria with low productivity. It seems therefore interesting to look for alternatives for their production. Y. lipolytica is a good alternative. This involves genetic modifications to enable these syntheses and the study of the influence of culture conditions. For this purpose, synthetic pathways of these two fatty acids as well as the elements that can influence their production are to be acquired. In this context, we tested several strains with different genetic background in flask and fermenter on neo-synthesis and bioconversion media. One strain was selected allowing reduction of CLA degradation by blocking the β-oxidation pathway and the better production of CLAs in fermenter with soybean oil by overexpression of FAD2 (302 mg. L-1). The influence of media composition was studied. The results showed better assimilation of Dynamis soy peptone rich in free amino acids by this yeast. This has a positive effect on growth and productions. In addition, the Fed-batch fermenter strategy allowed the optimal production of CLA (0.4 g. L-1), and nitrogen and phosphorus limitations seems to affect their accumulation. First generation strains producing CFA by expression of the CFA gene of E. coli were studied. They were tested on different culture media. Two strains (GY1005 and GY1070) were retained for their maximum oil production and/or CFA accumulation on neosynthesis medium in fermenter. This is due to DGA2 and GPD1 genes overexpression allowing a strong lipid accumulation and in the absence of remobilization and degradation of fatty acids. Media composition and culture parameters, such as strong stirring (1 000 rpm) and a Fed-batch strategy using a solution with the same composition as the starting medium, made it possible to maximize CFA production (1.2 g. L-1 for GY1070 and 0.9 g. L-1 for GY1005). A so-called second generation strain (JMY5578) was genetically optimized (CFA gene was expressed under the control of a stronger promoter and LRO1 over-expression gene). This strain was tested in flask and fermenter in order to evaluate the influence of culture medium compounds on CFA production. The results showed a gain of 30% CFA and 6% biomass in the presence of magnesium chloride and vitamins in the culture medium. In addition, a continuous addition of dextrose in fermenter and a nitrogen and phosphorus-rich starting medium allowed production of 3 g.L-1 of CFA. We also looked at the effect of promoters for the CFA gene expression in secondgeneration strains. Expression of the gene under the control of the php8d promoter gave the best result in terms of CFA accumulation in JMY6068 strain (46% in total lipids) and appears to be the strongest promoter in comparison with TEF
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Кічура, Марія Андріївна. "Технологія виробництва бактеріального концентрату закваски для кисломолочних продуктів. Дільниця виробничого біосинтезу." Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/41082.
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Дипломний проект містить 108 сторінок, 10 ілюстрацій, 3 таблиці, 2
креслиники та 109 бібліографічних найменувань за переліком посилань.
Метою даної роботи є проектування виробництва бактеріальної закваски
для виготовлення кисломолочних продуктів дієтичного харчування,зокрема
йогурту.
Робота присвячена збільшенню обсягів виробництва вітчизняних заквасок,
що дасть можливість збільшення асортименту та здешевлення молочнокислих
продуктів.
Обгрунтовано і запропоновано в якості продуцента бактеріальної закваски
використовувати штами гомоферментативних термофільних молочнокислих
бактерій Lactobacillus delbrueckii subsp. Вulgaricus та Streptococcus salivarius
subsp. Thermophilus, отримані у результаті селекції з використанням
природнього добору , мають пробітичні властивості і є високотехнологічними
для виготовлення кисломолочної пробдукції.
Обрано ефективне, економічно вигідне та надійне обладнання для
виробничого культивування закваски. Розраховано та обрано апарат для
виробничого культивування. Наведено технологічний, конструктивний та
гідравлічний розрахунки обраного ферментеру.
В роботі обґрунтовані та подані технологічна та апаратурна схеми
виробництва.The diploma project contains 108pages, 10 illustrations, 3 tables, 2 drawings and 109 bibliographic titles according to the list of references. The purpose of this work is to design the production of bacterial leaven for the manufacture of fermented milk products for dietary nutrition. The work is devoted to increasing the production of domestic leavens, which will increase the range and reduce the cost of lactic acid products. It is substantiated and proposed to use strains of homofermentative thermophilic lactic acid bacteria Lactobacillus delbrueckii subsp as a producer of bacterial yeast. Vulgaricus and Streptococcus salivarius subsp. Thermophilus, obtained by selection using natural selection, have probiotic properties and are high-tech for the manufacture of fermented milk production. Efficient, cost-effective and reliable equipment for production cultivation of sourdough has been selected. The device for industrial cultivation is calculated and selected. Technological, constructive and hydraulic calculations of the selected fermenter are given. The technological and hardware schemes of production are substantiated and presented in the work
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Bombardi, Franciele Mendes de Lima. "Sensoriamento ótico da dinâmica do crescimento de colônias de escherichia coli em ambiente hídrico." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2526.
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Este trabalho apresenta um estudo empregando duas técnicas óticas para monitorar o crescimento de culturas de cepas de Escherichia coli em dois meios de cultura líquidos: Espectroscopia de absorção UV-Vis (turbidimetria) e espectroscopia Raman. Na primeira técnica, a turbidez permite avaliar as diferentes fases naturais de crescimento de uma cultura bacteriana (lag, exponencial, estacionária e decaimento) por meio da densidade ótica, medida com um espectrômetro UV-VIS. Na segunda, o espalhamento Raman (medido com um espectrômetro dispersivo), a partir de amostras de água contaminada, fornece não apenas informações sobre as fases de crescimento, mas também abre a possibilidade de identificação bacteriana através da sua impressão digital característica. Mediu-se a dinâmica de duas cepas de E. coli – (nomeadas como H2/11 e H3C2/12) em um caldo líquido nutriente e quatro cepas de E. coli (nomeadas como H2/11, H3C2/12, 109 e 110) em caldo líquido EC, mantidas a 37,0°C ao longo de 24 horas. Alíquotas das amostras foram removidas da cultura em intervalos de tempo regulares para medições espectrais. A análise da turbidez permitiu medir o tempo de geração (isto é, o tempo de duplicação de uma população), que foi maior para cepas crescidas em caldo EC. Os espectros Raman forneceram informações sobre a evolução temporal das bandas a 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associadas com impressões digitais de componentes biológicos específicos. Os dados espectrais foram analisados por Análise de Componentes Principais (PCA). Os resultados obtidos em ambas as técnicas permitiram identificar as fases lag, exponencial e estacionária das cepas estudadas.This work is a study using two optical techniques to monitor the growth of cultures of Escherichia coli strains in two liquid culture medium: Raman spectroscopy and UV-Vis absorption spectroscopy (turbidimetry). In one hand, turbidity allows evaluating the different phases of growth of a bacterial culture (lag, exponential, stationary and decay) by optical density, measured with an UV-VIS spectrometer. On the other hand, Raman scattering (measured with a dispersive spectrometer) from contaminated water samples not only provides information about the grow phases, but also opens a possible identification of bacterial by its characteristic fingerprint. Two strains of E. coli (named as H2 / 11 and H3C2 / 12) were measured in liquid nutrient broth and four E. coli strains (named H2 / 11, H3C2 / 12, 109 and 110) in EC liquid broth, kept at 37.0 ° C over 24 hours. Aliquots of the samples were removed from the culture at regular time intervals for spectral measurements. The turbidity analysis allowed to measure the generation time, which was higher for strains grown in EC broth. Raman spectra provided information about the time evolution of the bands at 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associated with fingerprints of biological components. Data were analyzed by Principal Component Analysis (PCA). These results of both techniques allowed identifying the phases lag, exponential and stationary of the studied strains.
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Panzeca, Andrea. "You Don't Have to Be Good." ScholarWorks@UNO, 2015. http://scholarworks.uno.edu/td/1979.
Full textAbstract:
You Don't Have to be Good, is a nonfiction collection of prose, poetry and graphic memoir set in New Orleans, central Florida, and points in between. In this coming-of-age memoir, I recall the abrupt end of my dad's life, the 24 years of my life in which he was alive, and the years after his death—remembering him while living without him in his hometown of New Orleans. Along the way there are meditations on language, race, gender, dreams, addiction, and ecology. My family and I encounter Hurricane Katrina and Mardi Gras, and at least one shuttle launch. These are the stories I find myself telling at parties, and also those I've never voiced until now.
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Tsoulis-Reay, Alexa. "Convergence, concern and the "real" girl: teenage girls' everyday media cultures." 2009. http://repository.unimelb.edu.au/10187/4893.
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This is a revisionist audience study examining the everyday media cultures of twenty-four young teenage girls from Melbourne in Australia. It argues that in an era of proliferated and convergent media, audience studies cannot restrict its vision to a single media text, technology, or genre.It takes a broad approach to girls’ media culture and considers the full range of media that girls engage with on a daily basis. It identifies a hegemonic discourse about girls’ media use which it calls “(feminist) new media effects”. This anxiety takes as its key concern the proliferation of media and mediated representations of girls across the spaces of everyday life. (Feminist) new media effects discourse renders girls passive and unable to cope with such media presence without the guidance of adults to teach them how to correctly engage with the media. In order to challenge this construction, the thesis examines participants’ engagements with a range of convergent media texts and technologies, including Internet social networking, repeat DVD spectatorship, young female celebrities, and discourses of moral panic. It shows how mediated representations of girls across these sites are embedded in the fabric of participants’ everyday lives. Apart from highlighting the challenge that this poses to the practice of conducting audience research, it demonstrates the ways that girls both resist and incorporate mediated constructions of femininity within their everyday negotiations of teenage girlhood. It argues that the representation of girlhood constructed in (feminist) new media effects discourse – the vulnerable girl overwhelmed by toxic media messages – is key to girls’ media culture. My findings indicate that participants are primarily invested in resisting this construction of youthful femininity.
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Coover, Roderick Luis. "Visualizing cultures : the tropologies of montage and the ethnographic image /." 1999. http://wwwlib.umi.com/dissertations/fullcit/9943004.
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Thesis (Ph. D.)--University of Chicago, 1999."The videocassette is not included in this original manuscript. It is available for consultation at the author's graduate school library"--Prelim. Includes bibliographical references (leaves 3-7). Also available on the Internet.
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Phulukdaree, Alisa. "The effects of Sutherlandia frutescens in cultured renal proximal and distal tubule epithelial cells." Thesis, 2009. http://hdl.handle.net/10413/8570.
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Sutherlandia frutescens (SF), an indigenous medicinal plant to South Africa (SA), is traditionally used to treat a diverse range of illnesses including cancer and viral infections. The biologically active compounds of SF are polar, thus renal elimination increases susceptibility to toxicity. This study investigated the antioxidant potential, lipid peroxidation, mitochondrial membrane potential and apoptotic induction by SF on proximal and distal tubule epithelial cells. Cell viability was determined using the MTT assay. Mitochondrial membrane potential was determined using a flow cytometric JC-1 Mitoscreen assay. Cellular glutathione and apoptosis were measured using the GSH-GloTM Glutathione assay and Caspase-Glo® 3/7 assay, respectively. The IC50 values from the cell viability results for LLC-PK1 and MDBK was 15 mg/ml and 7 mg/ml, respectively. SF significantly decreased intracellular GSH in LLC-PK1 (p < 0.0001) and MDBK (p < 0.0001) cells. Lipid peroxidation increased in LLC-PK1 (p < 0.0001) and MDBK
(p < 0.0001) cells. JC-1 analysis showed that SF promoted mitochondrial membrane depolarization in both LLC-PK1 and MDBK cells up to 80%
(p < 0.0001). The activity of caspase 3/7 increased both LLC-PK1 (11.9-fold; p < 0.0001) and MDBK (2.2-fold; p < 0.0001) cells. SF at high concentrations plays a role in increased oxidative stress, altered mitochondrial membrane integrity and promoting apoptosis in renal tubule epithelia.Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2009.
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Zhi, Chunxing. "Cultivation of Laminaria saccharina gametophyte cell cultures and Acrosiphonia coalita tissue cultures in a bubble-column photobioreactor." Thesis, 1994. http://hdl.handle.net/1957/35536.
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Riley, Henry Drinker. "The characterization of the induction of lipocortin I by administration of dexamethasone and thyroid hormone in a thymic epithelial cell lne." Thesis, 1990. http://hdl.handle.net/10125/9372.
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Connon, Stephanie A. "Culturing uncultured environmental microorganisms." Thesis, 2002. http://hdl.handle.net/1957/31852.
Full textAbstract:
Research on natural environments, over the last decade, is replete with
microbial diversity studies that used culture-independent approaches. The cloning
and sequencing of the 16S rRNA genes has been the driving force in the expansion
of awareness about the great diversity of previously undiscovered microorganisms.
Well-known uncultured groups of microorganisms are numerous, and half of the
known phylogenetic divisions of bacteria are not represented in any culture
collection. It is no longer assumed that cultures acquired from an environment
represent the dominant or physiologically important organisms from that
environment. A high throughput culturing (HTC) technique was developed in an
attempt to bring into culture some of these widespread and uncultured
microorganisms. Over the course of 3 years, 2,484 culturing attempts were
screened for microbial growth from sample collections off the coast of Oregon and
576 attempts from groundwater at McClellan Air Force Base (MAFB). However,
using the HTC approach up to 14% of the microorganisms counted by direct
microscopy were cultured. In contrast, less than 1% of the microorganisms from
natural environments that are observed under a microscope can be grown using
standard agar plating techniques. This newly developed technique was successful
at bringing into culture 11 previously uncultured or undescribed Proteobacteria.
Four were isolated from the marine environment including, members of the SAR11
clade (alpha subclass), OM43 (beta-subclass), SAR92 (gamma subclass), and
OM60/OM241 (gamma subclass). SAR11 was transiently cultured in this study
but was later successfully brought into culture using these HTC techniques by Mike
Rapp��. Eight were isolated from a trichioroethene (TCE) and cis-dichloroethene
(cis-DCE) contaminated aquifer, including members of the MHP14 clade (alpha
subclass), 4-Org1-14 dade (alpha subclass), Herbaspirillum/Oxalobacter clade
(beta subclass), HTCC333 (beta subclass), HTCC410 (beta subclass), PM1 clade
(beta subclass), Boom-7m-04 clade (beta subclass) and OM43 clade (beta
subclass). Culturing microorganisms is an important step towards understanding
their physiology and ecology, and in most cases is necessary for the formal
systematic description of a new species. For microorganisms of global
significance, such as the major uncultured bacterioplankton and soil microbiota,
obtaining cultures is a prerequisite for obtaining complete genome sequences and
understanding the relevance of these microorganisms to biogeochemical cycles.Graduation date: 2003
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Mullikin, Ronald K. "Cultivation of suspension cultures of Laminaria saccharina (Phaeophyceae) gametophytes in tubular, planar, and stirred tank photobioreactors." Thesis, 1998. http://hdl.handle.net/1957/33749.
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Ramanan, Sundar. "Biomass productivity enhancement of Laminaria saccharina cultures in a stirred-tank bioreactor by batch and fed-batch nutrient delivery." Thesis, 1996. http://hdl.handle.net/1957/34286.
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Lin, Yu-Shuai, and 林余帥. "Studies on the culture conditions of Ganoderma lucidum in submerged cultures and the utilization of pineapple peel in the fermentation media." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/56138218369239319524.
Full textAbstract:
碩士東海大學
食品科學系
90
The purpose of this research is to investigate the influence of different environmental parameters and the components of the fermentation media on the mycelium growth and extracellular -polysaccharide production in submerged culture of Ganoderma lucidum, as well as utilization of fruit peel as substrates in the cultivation of this organism. Experiments were run on orbital shake flasks and 5 liter jar-fermentor. Results showed that the initial pH of the cuture medium was best at 4.0 and without illumination in the 7 days shake flasks fermentation. It had better mycelium growth and higher concentration of polysaccharide. But, the results had negative effect when the pH in the fermenting broth were re-adjusted during shake flasks fermentations. As a carbon source, sucrose was superior to glucose for polysaccharide production. Medium containing 3% sucrose could produce 0.470 mg/ml polysaccharide which is higher than (p<0.05) the 5% glucose (0.417mg/ml); A 4% fructose medium resulted in a mycelium concentration of 569.6 mg/100ml, which is higher than (p<0.05) 5% glucose (558.9 mg/100ml). Yeast extracts was the best among the organic nitrogen sources tested. Substrates containing 7% (w/v) pineapple peel favored mycelium growth, but depressed polysaccharide formation. Addition of 0.1%-0.5% ascorbic acid stimulated both mycelium growth and polysaccharide production to a maximum level of 702.1 mg/100ml and 0.786 mg/ml, respectively, as compared to the control of 440.7 mg/100ml and 0.394 mg/ml. Addition of citric acid, malic acid and gluconic acid showed negative effect for polysaccharide production. The polysaccharide obtained from the fermentor test were less than that of the shake flasks after 14 days culture. Controlled pH at 4.0 in the fermentor had reversed effect on the polysaccharide production when compared to the pH uncontrolled fermentation.
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"Evaluation of mageu-based gluten-free bread in South Africa." Thesis, 2015. http://hdl.handle.net/10210/13705.
Full textAbstract:
M.Tech. (Food Technology)Coeliac disease is an autoimmune disease triggered by the ingestion of gluten; persons suffering from coeliac disease are compelled to follow a life-long gluten-free diet. Gluten-free bread, (GFB), has poor quality attributes compared to wheat bread. The effect of mageu, a traditional beverage on quality parameters of GFB with and without selected hydrocolloids was studied. Mageu produced from maize flour and commercial starter cultures were used in GFB based on sorghum, soybean flour and maize starch. It is hypothesized that mageu with or without hydrocolloids could improve GFB quality aspects. The quality parameters measured were specific volume, loaf height, bake loss, rheological attributes, crumb firmness, firming rate, onset of mould growth and sensory attributes: texture, crumb colour, crust colour, flavour and overall acceptability.....