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Academic literature on the topic 'Epithelium Cultures and culture media'

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Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Epithelium Cultures and culture media"

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Johnson, L. G., K. G. Dickman, K. L. Moore, L. J. Mandel, and R. C. Boucher. "Enhanced Na+ transport in an air-liquid interface culture system." American Journal of Physiology-Lung Cellular and Molecular Physiology 264, no. 6 (June 1, 1993): L560—L565. http://dx.doi.org/10.1152/ajplung.1993.264.6.l560.

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Use of the air-liquid interface culture technique has produced improved morphological differentiation of rodent, canine, and human tracheal epithelia. We have investigated the effect of this culture technique on ion transport activities of cultured canine bronchial epithelia. These cells were isolated from excised airways by enzymatic digestion and plated on permeable collagen membrane substrates. All cultures were maintained utilizing standard culture techniques, by bathing both apical and basolateral sides with hormone supplemented, serum-free media until confluent (days 4–6). Half of the cultures were converted to air-liquid interface cultures (ALIC) by gentle aspiration of the apical medium and half were continued under standard technique culture (STC) conditions. After three additional days, preparations cultured under both conditions were mounted in modified Ussing chambers where bioelectric properties were measured under short-circuit conditions. Mean short-circuit current (Isc) was significantly greater in ALIC (-91.3 +/- 7.84 microA/cm2) than in STC (-54.8 +/- 5.03 microA/cm2). The sodium channel blocker, amiloride, reduced Isc by 68.4 +/- 5.0% in STC and by 84.8 +/- 3.0% in ALIC. 22Na and 36Cl fluxes confirmed the presence of enhanced sodium absorption in ALIC when compared with STC. The depth of the apical fluid, measured by microelectrodes during ALIC, was approximately 15 microns. Studies of cellular metabolism demonstrated a shift in metabolism from an anaerobic to an oxidative pattern in ALIC. This change in the pattern of metabolism suggests that the ALIC technique enhanced sodium transport in canine bronchial epithelia by increasing oxygen delivery to the epithelium.
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Senanayake, P. deS, A. Calabro, K. Nishiyama, J. G. Hu, D. Bok, and J. G. Hollyfield. "Glycosaminoglycan synthesis and secretion by the retinal pigment epithelium: polarized delivery of hyaluronan from the apical surface." Journal of Cell Science 114, no. 1 (January 1, 2001): 199–205. http://dx.doi.org/10.1242/jcs.114.1.199.

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Hyaluronan and chondroitin sulfate glycosaminoglycan secretion from retinal pigment epithelial cells was established in confluent cultures with high transepithelial resistance. Cell cultures were maintained on Millicell-PCF culture plates, which allow separation of culture medium exposed to apical and basal epithelial surfaces. Following various times in culture, apical and basal culture media were sampled at three day intervals and the glycosaminoglycan content was quantified. Samples were digested with proteinase K to free the glycosaminoglycans from their core proteins, the glycosaminoglycans were ethanol precipitated, and subjected to hyaluronidase SD and chondroitinase ABC digestion to release hyaluronan and chondroitin sulfate disaccharides. Disaccharides were fluorotagged with 2-aminoacridone, separated on polyacrylamide gels and the molar fluorescence in each disaccharide band quantitated. Hyaluronan in the apical medium was significantly higher than in the basal medium (5-12 times) at all recovery intervals (P<0.0001). In contrast, the distribution of unsulfated chondroitin, 4-sulfated chondroitin and 6-sulfated chondroitin disaccharides in apical and basal media was non-polar. Confocal microscopy of cultures probed with a hyaluronan-specific fluorotag established that the HA evident in these cultures is restricted to the apical border of the RPE cultures. Collectively, these data indicate that hyaluronan synthesized by the retinal pigment epithelium is secreted preferentially from the apical surface, suggesting that this tissue is an important source of hyaluronan present in the interphotoreceptor matrix.
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Morimoto, Keisuke, Hiroshi Mishima, Teruo Nishida, and Toshifumi Otori. "Role of Urokinase Type Plasminogen Activator (u-PA) in Corneal Epithelial Migration." Thrombosis and Haemostasis 69, no. 04 (1993): 387–91. http://dx.doi.org/10.1055/s-0038-1651617.

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SummaryThe role of plasminogen activator (PA) in the migration of comeal reepithelialization was studied. Rabbit corneal blocks were cultured, and both the extent of epithelial migration over the exposed corneal stroma and the activity of PA released into the culture media were measured. A significant, direct correlation between epithelial migration and PA activity in the medium was observed, even when the migration was stimulated by fibronectin or EGF, or was inhibited by cytochalasin B or cycloheximide. Zymography confirmed that the PA released into the culture medium was of the urokinase type (u-PA). Immunohistochemical studies showed that u-PA and plasmin(ogen) were present at the leading edge of the migrating epithelium. Studies of corneal cell cultures indicated that epithelial cells rather than endothelial cells or fibroblasts were the source of the u-PA. The addition of anti-human u-PA IgG or protease inhibitors retarded the migration of the comeal epithelium in a dose-dependent manner, indicating that u-PA activity is essential for the migration of the corneal epithelium. These findings suggest that the migration of corneal epithelial cells requires not only cell attachment to the extracellular matrix through the fibronectin but also degradation of the fibronectin by the release of cellular u-PA.
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Seeber, Judith W., Michaela Zorn-Kruppa, Simone Lombardi-Borgia, Heike Scholz, Anna K. Manzer, Brigitte Rusche, Monika Schäfer-Korting, and Maria Engelke. "Characterisation of Human Corneal Epithelial Cell Cultures Maintained under Serum-free Conditions." Alternatives to Laboratory Animals 36, no. 5 (November 2008): 569–83. http://dx.doi.org/10.1177/026119290803600512.

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Three-dimensional tissue constructs have been proposed as in vitro screening models for ocular irritancy. Based on our previous studies, in which a full-thickness corneal model based exclusively on SV40-immortalised cell lines was generated, we have currently evaluated the effects of a range of commercially-available cell culture media on several cellular parameters in cultures of a human corneal epithelial (HCE) cell line. This cell line was used in an attempt to establish a rational basis for the development of serum-free culture media for the assembly and long-term tissue culture of full-thickness corneal models. Briefly, we investigated the impact of serum-free culture on the proliferation, morphology, barrier function and cytokine expression of HCE cells. The number of cell layers and the epithelial differentiation were evaluated by histology. Barrier properties were characterised via the determination of transepithelial electrical resistance (TEER), fluorescein permeation, and the expression of the tight junction-related protein, zona occludin 1 (ZO-1). The cytokine expression pattern in response to serum-free culture was measured by using an antibody array system. Our results revealed that both the morphology and the barrier function of the epithelial constructs were comparable to those of human donor corneas, when serum-free media were supplemented with ascorbic acid, calcium, hydrocortisone and retinoic acid. Under these conditions, the artificial epithelium based on serum-free HCE cultures represented a valid model for the natural ocular surface.
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Borzenok, Sergey A., Boris E. Malyugin, Maxim Y. Gerasimov, Dmitriy S. Ostrovskiy, and Anna V. Shatskikh. "Feeder-Free Cell Culture of Labial Oral Mucosal Epithelium for Tissue-Engineering and Regenerative Medicine." Annals of the Russian academy of medical sciences 75, no. 5 (December 27, 2020): 561–70. http://dx.doi.org/10.15690/vramn1357.

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Background.The cultured cheek mucosa epithelium (buccal epithelium, BE) is used for autologous transplants generation and tissue engineering. An alternative source of cells for these purposes may be the lip mucosa, covered, like BE, with non-keratinized stratified squamous epithelium, but with some histological distinctions.Aims to characterize the human lip mucosa as a promising source of epithelial cells for autologous transplantation and tissue engineering.Methods.Scrapings of the lip, cheek, and gum mucosa from five healthy volunteers were analyzed by cytofluorimetry to determine the level of desquamation and cytokeratin (CK) 10 and 13 expression. The lip mucosa of two patients was characterized using routine histological staining and fluorescence immunohistochemistry for CK 3, 4, 10, 13, and p63 marker. 35 samples of full-thickness strips of the patients lip mucosa were used to set the explant (n=18) and enzymatic (n=17) techniques for expansion epithelium. Culture systems with 1.05 and 0.06mM Calcium contained 5% fetal bovine serum, 5 g/ml human insulin, 5 g/ml hydrocortisone, 10 ng/ml human epidermal growth factor. Stable cultures were stained for p63, vimentin, zonula occludens-1 (ZO-1), and CK10. Software tools determined levels of their expression.Results.The number of cells in the lip and gum samples was significantly lower than from the cheek. The median number of CK13 positive cells was significantly different for the gum (6.4%) and cheek (64.8%, p=0.0089). Significant differences for CK10 positive cells were not observed. The epithelium of the lip mucosa was 72.13.6 m thick, relatively flat, and without keratinization sites. Samples were positively stained for CK 4 and 13, in the absence of expression of CK 3 and 10. The primary culture of epithelial cells obtained by explant technique was significantly more effective (p=0.001) in comparison with the enzymatic method. Stable cultures had a cobble-stone morphology in both culture systems. The levels of vimentin and p63 expression in both culture systems was not significantly differ. ZO-1 expression was 3.6-fold higher for 1.05-mMCa++medium (p=0.0006).Conclusions.Epithelium cell culture from the lip mucosa can be obtained by culturing explants without a feeder layer. Quality control steps have been developed for cultured cells and biopsy site.
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Kelly, Scott P., and Chris M. Wood. "Effect of cortisol on the physiology of cultured pavement cell epithelia from freshwater trout gills." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 281, no. 3 (September 1, 2001): R811—R820. http://dx.doi.org/10.1152/ajpregu.2001.281.3.r811.

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Cortisol had dose-dependent effects on the electrophysiological, permeability, and ion-transporting properties of cultured pavement cell epithelia derived from freshwater rainbow trout gills and grown on cell culture filter supports. Under both symmetrical (L15 media apical/L15 media basolateral) and asymmetrical (freshwater apical/L15 media basolateral) culture conditions, cortisol treatment elevated transepithelial resistance, whereas permeability of epithelia to a paracellular permeability marker (polyethylene glycol-4000) decreased. Cortisol did not alter the Na+-K+-ATPase activity or the total protein content of the cultured preparations. During 24-h exposure to asymmetrical conditions, the net loss rates of both Na+ and Cl− to the water decreased with increasing cortisol dose, an important adaptation to dilute media. Unidirectional Na+ and Cl− flux measurements and the application of the Ussing flux-ratio criterion revealed cortisol-induced active uptake of both Na+ and Cl− under symmetrical culture conditions together with an increase in transepithelial potential (positive on the basolateral side). Under asymmetrical conditions, cortisol did not promote active ion transport across the epithelium. These experiments provide evidence for the direct action of cortisol on cultured pavement cell epithelia and, in particular, emphasize the importance of cortisol for limiting epithelial permeability.
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Romberger, D. J., P. Pladsen, L. Claassen, M. Yoshida, J. D. Beckmann, and S. I. Rennard. "Insulin modulation of bronchial epithelial cell fibronectin in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 2 (February 1, 1995): L230—L238. http://dx.doi.org/10.1152/ajplung.1995.268.2.l230.

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Fibronectin (Fn) is involved in the migration of epithelial cells in re-epithelialization of wounds. Epithelial cell-derived Fn is particularly potent as a chemotactic factor for bronchial epithelial cells (BECs) in vitro. Thus modulation of airway epithelial cell Fn may be a key aspect of airway repair. Insulin is both an important growth factor and known chemotactic factor for cultured BECs. We postulated that insulin may modulate Fn production of cultured BECs. We examined this hypothesis utilizing bovine BECs in culture with serum-free media with and without insulin. BECs grown in media without insulin released more Fn into culture supernatants and contained more Fn in cell layers than cells grown with insulin. Labeling of cells with [35S]methionine demonstrated an increase in new protein production and Fn mRNA expression was increased. Increased Fn in BEC cultures without insulin was associated with an increase in active transforming growth factor-beta (TGF-beta) release as measured by a standard bioassay. Increased BEC Fn in cultures without insulin was partially inhibited by exposure of cultures to TGF-beta antibody. Thus insulin appears to modulate BEC Fn production in vitro in part through a TGF-beta-dependent mechanism. Insulin may be involved in airway repair mechanisms through modulation of epithelial cell Fn production.
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Chato-Astrain, Jesús, David Sánchez-Porras, Óscar Darío García-García, Claudia Vairo, María Villar-Vidal, Silvia Villullas, Indalecio Sánchez-Montesinos, Fernando Campos, Ingrid Garzón, and Miguel Alaminos. "Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers." Biomedicines 9, no. 11 (November 6, 2021): 1634. http://dx.doi.org/10.3390/biomedicines9111634.

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Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these cultures in less time. In the present study, we evaluated the effect of novel formulations based on EGF-loaded nanostructured lipid carriers (NLC). First, biosafety of NLC containing recombinant human EGF (NLC-rhEGF) was verified in immortalized skin keratinocytes and cornea epithelial cells, and in two epithelial cancer cell lines, by quantifying free DNA released to the culture medium. Then we established primary cell cultures of human skin keratinocytes with basal culture media (BM) and BM supplemented with NLC-rhEGF, liquid EGF (L-rhEGF), or NLC alone (NLC-blank). The results showed that cells isolated by enzymatic digestion and cultured with or without a feeder layer had a similar growth rate regardless of the medium used. However, the explant technique showed higher efficiency when NLC-rhEGF culture medium was used, compared to BM, L-rhEGF, or NLC-blank. Gene expression analysis showed that NLC-rhEGF was able to increase EGFR gene expression, along with that of other genes related to cytokeratins, cell–cell junctions, and keratinocyte maturation and differentiation. In summary, these results support the use of NLC-rhEGF to improve the efficiency of explant-based methods in the efficient generation of human keratinocyte primary cell cultures for tissue engineering use.
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Ferrara, N., D. K. Fujii, P. C. Goldsmith, J. H. Widdicombe, and R. I. Weiner. "Transport epithelial characteristics of cultured bovine pituitary follicular cells." American Journal of Physiology-Endocrinology and Metabolism 252, no. 3 (March 1, 1987): E304—E312. http://dx.doi.org/10.1152/ajpendo.1987.252.3.e304.

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Confluent monolayers of polygonal epithelioid cells were obtained from enzymatically and mechanically dispersed bovine anterior pituitaries (AP) and pars tuberali (PT). The ultrastructure of the cells composing the monolayer was consistent with the follicular or folliculostellate cells (FC) of the pituitary, i.e., lack of secretory granules; formation of follicles in culture; interdigitations with neighboring cells with numerous tight junctions; presence of extensive microfilaments; and sparse rough endoplasmic reticulum and Golgi apparatus. Culture media from monolayers of first passage cultures contained little if any of the AP hormones' luteinizing hormone, prolactin, and ACTH. Shortly after reaching confluency, regions of the monolayer bulge away from the surface of the culture dish to form domes. Dome formation has been described only with cultures of cells that function as transport epithelia in vivo. FC cultured on polycarbonate filters were placed in Ussing chambers. A transepithelial potential difference of approximately 1.1 mV and a resistance greater than 300 omega cm2 were detectable 4–5 days after plating. The short-circuit current (Isc) was decreased 70% by amiloride applied to the mucosal surface and further decreased by the addition of ouabain at the serosal surface. The beta-adrenergic agonist isoproterenol increased the Isc and this action was prevented by a beta-antagonist. These observations indicate that pituitary FC in culture behave as a transport epithelium. Considering the organization of FC in the AP and PT, they suggest a regulatory role for FC in the maintenance of the ionic composition of the interstitial fluid of the pituitary gland.
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Kouthouridis, Sonya, Julie Goepp, Carolina Martini, Elizabeth Matthes, John W. Hanrahan, and Christopher Moraes. "Oxygenation as a driving factor in epithelial differentiation at the air–liquid interface." Integrative Biology 13, no. 3 (March 2021): 61–72. http://dx.doi.org/10.1093/intbio/zyab002.

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Abstract Culture at the air–liquid interface is broadly accepted as necessary for differentiation of cultured epithelial cells towards an in vivo-like phenotype. However, air–liquid interface cultures are expensive, laborious and challenging to scale for increased throughput applications. Deconstructing the microenvironmental parameters that drive these differentiation processes could circumvent these limitations, and here we hypothesize that reduced oxygenation due to diffusion limitations in liquid media limits differentiation in submerged cultures; and that this phenotype can be rescued by recreating normoxic conditions at the epithelial monolayer, even under submerged conditions. Guided by computational models, hyperoxygenation of atmospheric conditions was applied to manipulate oxygenation at the monolayer surface. The impact of this rescue condition was confirmed by assessing protein expression of hypoxia-sensitive markers. Differentiation of primary human bronchial epithelial cells isolated from healthy patients was then assessed in air–liquid interface, submerged and hyperoxygenated submerged culture conditions. Markers of differentiation, including epithelial layer thickness, tight junction formation, ciliated surface area and functional capacity for mucociliary clearance, were assessed and found to improve significantly in hyperoxygenated submerged cultures, beyond standard air–liquid interface or submerged culture conditions. These results demonstrate that an air–liquid interface is not necessary to produce highly differentiated epithelial structures, and that increased availability of oxygen and nutrient media can be leveraged as important strategies to improve epithelial differentiation for applications in respiratory toxicology and therapeutic development.
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Dissertations / Theses on the topic "Epithelium Cultures and culture media"

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Thornton, Sarah. "Record hops to raves : authenticity and subcultural capital in music and media cultures." Thesis, University of Strathclyde, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261836.

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Tsoulis-Reay, Alexa. "Convergence, concern & the "real" girl : teenage girls' everyday media cultures /." Connect to thesis, 2009. http://repository.unimelb.edu.au/10187/4893.

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Rivas, Cecilia Maribel. "Imaginaries of transnationalism media and cultures of consumption in El Salvador /." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2007. http://wwwlib.umi.com/cr/ucsd/fullcit?p3258783.

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Thesis (Ph. D.)--University of California, San Diego, 2007.
Title from first page of PDF file (viewed June 8, 2007). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 159-168).
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林結美 and Kit-mei Lam. "Phenotypic and molecular characterization of a blood culture isolate of the family coriobacteriaceae." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42904274.

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Ferreira, Ana Raquel Santos. "A systems biology framework for pathway level culture media engineering: pplication to Pichia pastoris cultures." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/9369.

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Dissertação para obtenção do Grau de Doutor em Engenharia Química e Bioquímica
Culture media (CM) formulations contain hundreds of ingredients in aqueous solutions that may be involved in complex interactions in the same or competing pathways within the cell. This thesis proposes a new methodology for determining the optimal composition of CM that migrates from an empirical to a mechanistic or hybrid mechanistic CM development approach. A framework consisting in the execution of an array of cell cultures, endpoint exometabolomic assays and bioinformatics algorithm were brought together into a platform for CM engineering called Cell Functional Enviromics. This technology consists of a largescale reverse engineering approach that reconstructs cellular function on the basis of measured dynamic exometabolome data. To support this concept, a computational algorithm, called “envirome-guided Projection to Latent Pathways”, was developed. This method yields envirome-wide Functional Enviromics Maps (FEM), with rows representing medium factors, columns representing elementary (orthogonal) cellular functions and color intensity values, the strength of up-/down- regulation of cellular functions by medium factors. This method was applied to optimize Pichia Trace Metal salts for the yeast Pichia pastoris to improve the expression of heterologous proteins. An array of shake flasks experiments of the P. pastoris X33 strain were performed and used to build a FEM. Then, optimized CM formulations were calculated targeting predefined single-chain Fragment variable antibody (scFv) production improvements. Experimental validation shows a scFv productivity increase of approximately twofold, in relation to the control BSM recipe proposed by Invitrogen. These results were further validated in 2 L bioreactor experiments. Thereafter, scale-up to 50 L bioreactors was developed a mathematical model for further optimization of BSM salts in experiments of P. pastoris GS115. Direct adaptive (DO)-stat feeding controller that maximizes glycerol feeding through the regulation of DO concentration at 5% of saturation was developed and applied to the 50 L bioreactor, with the fully optimized CM composition.
Fundação para a Ciência e Tecnologia - bolsa de doutoramento SFRH/BD/36285/2007
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Pierre-Charles, Nathalie. "La construction du sens chez des jeunes de cultures diverses lors de la réception de messages médiatiques." Thesis, Paris 13, 2014. http://www.theses.fr/2014PA131043/document.

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Cette étude sur la compréhension des jeunes face aux messages médiatiques vise : - à mettre en évidence les apports des études de réception et plus précisément les capacités et les besoins des récepteurs médiatiques, le cadre de réception, la nature du public et les médiations socioculturelles ; - reconstituer la capacité, la compétence et l’intention communicationnelles du récepteur médiatique et la rhétorique de la réception ; - poser le cadrage pratique de notre étude par la nature scientifique et le type des messages proposés. Nous nous intéressons à la construction du sens par des jeunes de cultures diverses lors de la réception de messages scripturaux, audiovisuels et audio scriptovisuels. Nous traitons les données collectées par l’observation et le questionnement à la fois quantitativement et qualitativement. Les techniques de recueil de données retenues sont le questionnaire et l’observation. Problématiser notre objet de recherche revient à se poser la question suivante : quelle est la compréhension des jeunes face à la réception des messages médiatiques ? Nous organisons notre recherche autour de deux axes : - le jeune construit sa compréhension du message médiatique ; - le culturel et le social ont un impact sur la compréhension du message médiatique par le jeune
This study on the understanding of young people facing media messages aims: -to highlight the contributions of reception studies and specifically capabilities and the needs of media receivers, the approval framework, the nature of the public andsociocultural mediations; -restore the capacity, competence and the communicative intention of the media receiverand rhetoric of reception; -ask the practical framing of our study by the scientific nature and type of the proposedposts. We are interested in the construction of meaning by young people from various cultures during the reception of messages scriptural, audio-visual and audio disposed. We treat data collected through observation and questioning both quantitatively and qualitatively. Selected data collection techniques are the questionnaire and observation.Problematize our object of research is to ask the following question: what is the understanding of young people in receipt of media messages? We organize our research around two axes: -the young built his understanding of the media message.-the cultural and social impact on the understanding of the media message by the young
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Zimm, Malin. "Losing the plot : architecture and narrativity in fin-de siècle media cultures." Doctoral thesis, KTH, Architecture, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-481.

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This thesis investigates the role of the term plot in mediating relations between architecture and narrativity. Examining organisational strategies in the creation of real and virtual spaces, it identifies literary works by novelists who have resisted, or subverted, plot conventions in fiction (Joris-Karl Huysmans, Edmond de Goncourt, Xavier de Maistre and Neal Stephenson), and introduces architectural spaces such as Thomas Edison’s film-studio Black Maria, and the plotless productions of early cinematography, to juxtapose concepts of plot and spatiality in a study of the production and consumption of pre-digital virtual spaces. Plot here relates therefore both to narrative sequentiality and spatial organisation – from "storyline" to "ground plan". The "plotless" narrative structure of Huysmans, Goncourt and de Maistre focuses on the interaction between man – the "writerin- residence" – and his domestic interior, functioning as an excitant or stimulant for the production of both material and imagined spaces. The media culture of late 19th century society saw the first significant attempts at moving image technology and its related spatialities – the Black Maria, the kinetoscope, the kinetograph, and the films produced by these, which had yet to find a narrative form. The architecture of the plotless novels and the proto-cinematic experiments of the late 19th century modulate between physical reality and fiction. They are ripe in their descriptive narrativity, expanding in the imagination of the consumer. Stephenson’s imaginative transposition of book media into a "Primer" – a new form of narrative media that develops its narrative content directly from the environmental context of its reader – concludes the discussion of the thesis, highlighting interrelations between fictive and real space, influencing both writer and reader. The refusal of narrative plot deprives the reader of causality, but emphasises the fictitious spatial creation in which the reader becomes immersed. These spaces, by virtue of their disengagement from plot, allow us to revisit the possibilities of virtual space without common preconceptions concerning the creation or experience of digital mediating technology.

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Wonnah, Samson. "Myths, Risks, and Ignorance: Western Media and Health Experts’ Representations of Cultures in Ebola-Affected West African Communities." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3389.

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The 2014 Ebola outbreak, mostly affecting Liberia, Sierra Leone, and Guinea, is the largest ever recorded. The Ebola response encountered resistance in some affected communities, where some residents accused relief agencies from the Global North of denigrating local cultures. This thesis examines mainstream Western media and health experts’ representation of culture in the Ebola-affected region and employed Foucauldian analysis of discursive power to discuss the impact of such a representation on the concerned communities. Through a content analysis of selected journal and news articles by Western scholars and media and official reports by some relief agencies involved with the Ebola response, the study discovers evidence of culture bias. There was a use of significantly negative words in describing aspects of culture in the Ebola-affected region. Western media and health experts also largely associated the epidemic with African “backwardness.”
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Rogan, Frances. "Social media, bedroom cultures and femininity : exploring the intersection of culture, politics and identity in the digital media practices of girls and young women in England." Thesis, University of Birmingham, 2018. http://etheses.bham.ac.uk//id/eprint/8199/.

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In recent years, the position of (post-)millennial girls and young women within the digital landscape of social media has proven to be a topic of much interest to a number of feminists, journalists and cultural commentators. On the one hand, girls’ (social) media practices are presented as a key site of concern, wherein new digital technologies are said to have produced an intensification of individualized, neoliberal and post-feminist identities. At the same time, others have championed access to social media for young people as a revolutionary political tool, wherein previously marginalised political subjects (such as girls) can access and participate within new and exciting political cultures. This thesis offers an original contribution to these debates by locating itself at the intersection of these two approaches and examining the role of social media in the production of girls’ cultural and political identities. I present my findings from focus groups carried out with girls (aged 12-18) in three urban locations in England. This data is organised around the three overriding themes of space, surveillance and visibility. Ultimately, the thesis argues that social media should be conceptualised as an important terrain upon which neoliberal and postfeminist subjectivities can be both reproduced and subverted.
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Szemiel, Agnieszka M. "Replication of Bunyamwera virus in mosquito cells." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2570.

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The Bunyaviridae family is one of the largest among RNA viruses, comprising more than 350 serologically distinct viruses. The family is classified into five genera, Orthobunyavirus, Hantavirus, Nairovirus, Phlebovirus, and Tospovirus. Orthobunyaviruses, nairoviruses and phleboviruses are maintained in nature by a propagative cycle involving blood-feeding arthropods and susceptible vertebrate hosts. Like most arthropod-borne viruses, bunyavirus replication causes little damage to the vector, whereas infection of the mammalian host may lead to death. This situation is mimicked in the laboratory: in cultured mosquito cells no cytopathology is observed and a persistent infection is established, whereas in cultured mammalian cells orthobunyavirus infection is lytic and leads to cell death. Bunyaviruses encode four common structural proteins: an RNA-dependent RNA polymerase, two glycoproteins (Gc and Gn), and a nucleoprotein N. Some viruses also code for nonstructural proteins called NSm and NSs. The NSs protein of the prototype bunyavirus, Bunyamwera virus, seems to be one of the factors responsible for the different outcomes of infection in mammalian and mosquito cell lines. However, only limited information is available on the growth of bunyaviruses in cultured mosquito cell lines other than Aedes albopictus C6/36 cells. Here, I compared the replication of Bunyamwera virus in two additional Aedes albopictus cell clones, C7-10 and U4.4, and two Aedes aegypti cell clones, Ae and A20, and investigated the impact of virus replication on cell function. In addition, whereas the vertebrate innate immune response to arbovirus infection is well studied, relatively little is known about mosquitoes’ reaction to these infections. I investigated the immune responses of the different mosquito cells to Bunyamwera virus infection, in particular antimicrobial signaling pathways (Toll and IMD) and RNA interference (RNAi). The data obtained in U4.4 cells suggest that NSs plays an important role in the infection of mosquitoes. Moreover infection of U4.4 cells more closely resembles infection in Ae and A20 cells and live Aedes aegypti mosquitoes. My data showed that the investigated cell lines have various properties, and therefore they can be used to study different aspects of mosquito-virus interactions.
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Books on the topic "Epithelium Cultures and culture media"

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Ian, Freshney R., ed. Culture of epithelial cells. New York: Wiley-Liss, 1992.

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Michael, Skovmand, and Schrøder Kim, eds. Media cultures: Reappraising transnational media. London: Routledge, 1992.

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Inc, ebrary, ed. Domestic cultures. Maidenhead: Open University Press, 2008.

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C, Parks Lawrence, ed. Handbook of microbiological media. 2nd ed. Boca Raton: CRC Press, 1997.

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C, Parks Lawrence, ed. Handbook of microbiological media. Boca Raton: CRC Press, 1993.

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1948-, Crouch David, Jackson Rhona 1949-, and Thompson Felix 1951-, eds. The media and the tourist imagination: Converging cultures. London: Routledge, 2005.

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Dovey, Jon. Game cultures: Computer games as new media. Maidenhead, Berkshire, England: Open University Press, 2006.

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Atlas, Ronald M. Handbook of microbiological media. 4th ed. Boca Raton: Taylor & Francis, 2010.

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Hepp, Andreas. Cultures of mediatization. Cambridge, UK: Polity, 2013.

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Digestive Ferment Company. Difco Laboratories, Detroit. Difco manual: Dehydrated culture media and reagents for microbiology. Detroit: DIFCO Laboratories, 1994.

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Book chapters on the topic "Epithelium Cultures and culture media"

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Gstraunthaler, G., T. Seppi, C. Monteil, E. Healy, M. P. Ryan, J. P. Morin, and W. Pfaller. "The impact of growth conditions, culture media volume and glucose content on differentiation and metabolism of renal epithelial tissue cultures." In Ersatz- und Ergänzungsmethoden zu Tierversuchen, 265–66. Vienna: Springer Vienna, 1998. http://dx.doi.org/10.1007/978-3-7091-7500-2_45.

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Barnes, David. "Hormonally Defined, Serum-Free Media for Epithelial Cells in Culture." In Tissue Culture of Epithelial Cells, 235–53. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4814-6_12.

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Parham, John. "Green Journalism and Green Cultures." In Green Media and Popular Culture, 96–122. London: Macmillan Education UK, 2016. http://dx.doi.org/10.1007/978-1-137-00948-7_4.

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Barker, Thomas. "Making National Cultures." In The Handbook of Diasporas, Media, and Culture, 295–309. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2019. http://dx.doi.org/10.1002/9781119236771.ch20.

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Selvick, Stephanie. "Androgynous Social Media and Visual Culture." In Queer Youth and Media Cultures, 170–81. London: Palgrave Macmillan UK, 2014. http://dx.doi.org/10.1057/9781137383556_12.

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Osgerby, Bill. "Global media, local youth cultures, and hybridity." In Youth Culture and the Media, 153–74. Second Edition. | new york : routledge, 2020.: Routledge, 2020. http://dx.doi.org/10.4324/9781351065269-8.

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Moewaka Barnes, Helen, Patricia Niland, Lina Samu, Acushla Deanne Sciascia, and Tim McCreanor. "Ethnicity/culture, alcohol and social media." In Youth Drinking Cultures in a Digital World, 80–98. Abingdon, Oxon ; New York, NY : Routledge, 2017. | Series:: Routledge, 2017. http://dx.doi.org/10.4324/9781315660844-6.

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Unger, Alexander. "Modding as Part of Game Culture." In Computer Games and New Media Cultures, 509–23. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-2777-9_32.

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Cruz, Edgar Gómez, and Ignacio Siles. "Digital Cultures." In The Routledge Handbook to the Culture and Media of the Americas, 319–29. Abingdon, Oxon ; New York, NY : Routledge, 2020.: Routledge, 2020. http://dx.doi.org/10.4324/9781351064705-28.

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Berkin, Sarah Corona, and Sebastian Thies. "Visual cultures." In The Routledge Handbook to the Culture and Media of the Americas, 468–78. Abingdon, Oxon ; New York, NY : Routledge, 2020.: Routledge, 2020. http://dx.doi.org/10.4324/9781351064705-45.

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Conference papers on the topic "Epithelium Cultures and culture media"

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Groux, Kassandra, Anna Verschueren, Mathias Fink, Claude Boccara, Sacha Reichman, and Kate Grieve. "Detecting subcellular dynamic behaviours with dynamic full-field OCT on stressed retinal pigment epithelium cell cultures." In Optical Coherence Imaging Techniques and Imaging in Scattering Media, edited by Maciej Wojtkowski, Yoshiaki Yasuno, and Benjamin J. Vakoc. SPIE, 2021. http://dx.doi.org/10.1117/12.2616003.

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Telpov, Roman E. "Physical And Geographical Identity Features In Local Media Texts." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.104.

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Serebrennikova, Evgenia F. "Transnational Cognitive Units As Attractive Signs Of The Media Dialogue." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.93.

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Korotkevich, Daria O. "A Foreign Country As A Subject Of American Mass Media Discourse." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.46.

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Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

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Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. In particular, when culturing cells within hydrogel scaffold-filled networks, as may be done in micro-tissue engineering, the need for perfusion culture also increases the likelihood of a destructive bubble entering the network. Moreover, analysis of micro-cultures frequently entails labelling with antibodies and/or fluorescent probes, which again requires controlled perfusion of the various reagents through the network. We have developed a simple technique to preserve cell viability and simplify labeling within microscale cultures without the need for perfusion. Instead of bonding a microfluidic network to glass, PDMS, or another impermeable substrate, the network is bonded to a semi-permeable microdialysis membrane, which allows free exchange of oxygen, proteins, nutrients, and waste between the microfluidic channels and culture media in static culture plates.
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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarrier to microcarrier culture can be achieved with careful management of culture conditions and brief exposure to enzymes.Human umbilical artery and vein, and saphenous vein endothelial cells were prepared and grogn on microcarrier cultures to cell populations of 200-450 x 106 cells and conditioned for 14 day periods in serum-free media.The production profiles of several endothelial cell proteins including thrombospondin (TSP), von Willebrand Factor (vWF) and issue plasminogen activator (t-PA) were measured by radioimmunoassay under these conditions, and demonstrate the use of microcarrier cultures in producing milligram quantities of engothelial cell protein. For example, a HUVEC culture of 200 x 106 cells conditioned with serum-free media for 14 days yielded a total of 6.9mg TSP, 0.7mg vWF and 48.9ug t-PA. In this laboratory one such application of the system was the purification of endothelial proteins in amounts sufficient for immunisation of mice prior to the production of monoclonal antibodies and for subsequent characterisation.
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Merryman, W. David, Kristen Loveless, and Mehran Kasra. "Disc Nucleus Cellular Response to Dynamic Pressures at Critical Frequencies: A Pig Model." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-43092.

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Disc degeneration is a multifactor phenomenon. It has been found that intervertebral disc (IVD) cells respond to such factors as pH, osmotic pressure, genetic factors, and mechanical loading (Guilak, 1999). During daily activities the human intervertebral disc is exposed to oscillatory hydrostatic loads that produce pressures >2MPa in vivo (Nachemson, 1964 and 1979). It is known that dynamic loads with critical frequencies close to that of the in vivo human spine resonant frequency (4–5 Hz) have a destructive effect on disc tissue properties (Pope, 1993). Whether this destructive effect is purely mechanical, due to loading magnification, or biological, affecting cell metabolism, is unknown. Previous work (Merryman, 2002) showed that there was no significant effect upon monolayer IVD cells loaded at 15Hz, while lower frequencies (1 and 8Hz) altered collagen synthesis compared to control. To address this issue, we developed a mechanically active culture system capable of delivering a wide range of loading frequencies and amplitudes of hydrostatic pressure to cultures of disc cells. Nucleus pulposus cells of pig discs were isolated and suspended in alginate beads. Alginate cultures were divided into 6 groups; five groups were exposed to cyclic pressures of frequencies 1, 3, 5, 8, and 10Hz with the same amplitude of 1MPa, and group 6 was the control group (no loading). Cultures of different groups were loaded for 3 days (30 minutes daily) in a hydraulic chamber filled with culture media. The effect of loading frequency on collagen metabolism among different groups was compared by measuring incorporated [3H]-proline into collagen for medium and total extracts. The results indicated a poor synthesis rate and more degradation near the 5Hz frequency.
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Thiyagarajan, Magesh. "Portable Plasma Medical Device for Infection Treatment and Wound Healing." In ASME 2011 6th Frontiers in Biomedical Devices Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/biomed2011-66031.

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The purpose of this study was to determine the effects of plasma treatment on bacteria in liquid phases. We predict that the plasma gas can penetrate the liquid culture media and plasma treatment will efficiently kill the bacteria at unique time and distance parameters. It is also hypothesized that less stringent plasma treatment will negatively affect the growth rate of some species of bacteria and possibly their pathogenicity. The bacteria were exposed to hot and cold plasma at various time lengths and distance parameters. Our results indicated that 2 minutes of hot plasma treatment with the plasma torch 5 cm away from the liquid culture is effective in killing/sterilizing cultures of S. aureus, S. pyogenes, Salmonella spp, N. meningitidis, and E. coli. Five minutes of cold plasma with the probe immersed 1–2 cm inside the liquid culture were needed to kill the bacteria. The portable nonthermal plasma system can be used for infection treatment and wound healing applications affected by the microbes studied in this research [1–4].
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Momčilović, Jovana, Dragana Jakovljević, Milica Kanjevac, and Biljana Bojović. "FIZIOLOŠKE KARAKTERISTIKE RASTENJA PŠENICE (Triticum aestivum L.) U USLOVIMA IN VITRO." In XXVII savetovanje o biotehnologiji. University of Kragujevac, Faculty of Agronomy, 2022. http://dx.doi.org/10.46793/sbt27.503m.

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This study aimed to examine the effect of different media - Murashige Skoog (MS) and Gamborg (B5) on the growth and development of in vitro seedling cultures of wheat (Triticum aestivum L.). The effects were evaluated through the measurement of root and shoot length, fresh and dry mass, as well as through the determination of the concentration of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids). The obtained data indicate that MS has better effects on the growth and development of wheat seedlings, since longer shoot length, and better fresh weight were observed on seedlings from this type of media. Additionally, higher chlorophyll b concentration and lower carotenoid concentration were measured in wheat leaves grown on MS medium. It can be concluded that MS is more suitable for establishing the initial in vitro culture of wheat compared to the B5 medium.
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Ferdes, Mariana, and Rodica Roxana Constantinescu. "Isolation and characterization of fungal and bacterial proteolytic strains from chrome shavings." In The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.9.

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The chrome shavings waste obtained as a result of the leather finishing process accumulates in a large volume in tanneries and represent a major problem for the environment. This waste are particularly resistant to attack of microorganisms, due to the significant concentration of chromium and are thus difficult to degrade. In this study, chrome shavings were analyzed microbiologically by determining the total number of germs and the number of yeasts and molds on specific culture media. Several bacterial and fungal strains were isolated from the cultures in Petri dishes, after the growth of the colonies. These strains were characterized in terms of the production of proteolytic enzymes, by a method of screening on the media with casein, which allows the determination of proteolytic indices of microorganisms. As a result of the tests performed, five bacterial strains probably belonging to the genus Bacillus and two fungal strains from the genera Penicillium and Cladosporium were selected.
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Reports on the topic "Epithelium Cultures and culture media"

1

Hrytsenko, Olena. Sociocultural and informational and communication transformations of a new type of society (problems of preserving national identity and national media space). Ivan Franko National University of Lviv, February 2022. http://dx.doi.org/10.30970/vjo.2022.51.11406.

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The problems of the correlation of cosmopolitan and national identities are too complex to be unambiguous assessment, let alone alternative values (related to the ecological paradigm and the spiritual traditions of other cultures). However, it is obvious that without preserving the national identity, the integrity and independence of the national state becomes problematic. On the other hand, without taking into account the consequences of information wars and aggressive cosmopolitan tendencies of global media culture, there is a threat of losing the national information space and displacing it to the periphery of socio-political and economic life in Ukraine and in the modern world. In the process of working on research issues, the author of the article came out on the principles of objectivity, systematic and determinism, which in combination of their observance made it possible to determine the influence of the post-industrial information society on the formation of a new type of mass consciousness. As a result of the influence of globalization processes, there was a filling of the domestic information space with a supernational mass culture of entertainment, which in most cases leads to the spread of a primitive world outlook based on the ideology of consumption society, without leaving places to preserve sociocultural traditions and national identity. Therefore, given the problems of preserving national identity, it is necessary should be mentioned the information security of the state, which occupies one of the most important places, among various aspects of information security, since the unresolved problem of protection of the national information space significantly complicates the processes of formation of national identity.
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