Academic literature on the topic 'Environmental microbial isolates'
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Journal articles on the topic "Environmental microbial isolates"
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Pacholak, Amanda, Wojciech Smułek, Agnieszka Zgoła-Grześkowiak, and Ewa Kaczorek. "Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains." International Journal of Environmental Research and Public Health 16, no. 9 (April 30, 2019): 1526. http://dx.doi.org/10.3390/ijerph16091526.
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The continuous exposure of living organisms and microorganisms to antibiotics that have increasingly been found in various environmental compartments may be perilous. One group of antibacterial agents that have an environmental impact that has been very scarcely studied is nitrofuran derivatives. Their representative is nitrofurantoin (NFT)—a synthetic, broad-spectrum antibiotic that is often overdosed. The main aims of the study were to: (a) isolate and characterize new microbial strains that are able to grow in the presence of NFT, (b) investigate the ability of isolates to decompose NFT, and (c) study the impact of NFT on microbial cell properties. As a result, five microbial species were isolated. A 24-h contact of bacteria with NFT provoked modifications in microbial cell properties. The greatest differences were observed in Sphingobacterium thalpophilum P3d, in which a decrease in both total and inner membrane permeability (from 86.7% to 48.3% and from 0.49 to 0.42 µM min−1) as well as an increase in cell surface hydrophobicity (from 28.3% to 39.7%) were observed. Nitrofurantoin removal by selected microbial cultures ranged from 50% to 90% in 28 days, depending on the bacterial strain. Although the isolates were able to decompose the pharmaceutical, its presence significantly affected the bacterial cells. Hence, the environmental impact of NFT should be investigated to a greater extent.
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Roane, T. M., and S. T. Kellogg. "Characterization of bacterial communities in heavy metal contaminated soils." Canadian Journal of Microbiology 42, no. 6 (June 1, 1996): 593–603. http://dx.doi.org/10.1139/m96-080.
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Heavy metal pollution is a principle source of environmental contamination. We analyzed heavy metal impacted soil microbial communities and found that, in general, although lead adversely affected biomass, metabolic activity, and diversity, autochthonous lead- and cadmium-resistant isolates were found. In several metal-stressed soils, the microbial community consisted of two populations, either resistant or sensitive to lead. Additionally, a lead-resistant isolate was isolated from a control soil with no known previous exposure to lead, suggesting widespread lead resistance. Lead-resistant genera isolated included Pseudomonas, Bacillus, Corynebacterium, and Enterobacter species. Plasmids, ranging from 5 to 260 kb, were not detected through standard purifications from lead-resistant isolates. Positive correlations existed between antibiotic resistance and isolation habitat for lead-resistant strains, microbial metabolic activity and soil type, soluble lead concentration and microbial diversity, and arsenic concentration and total or viable cell concentrations.Key words: heavy metal, lead, cadmium, soil bacteria, stress, remediation.
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Pattani, Vivek B. "Characterization of Plant Growth-Promoting Activity of Bacteria Isolated from Forest and Coastal Regions of Saurashtra, Gujarat, India." Bioscience Biotechnology Research Communications 15, no. 1 (March 25, 2022): 144–51. http://dx.doi.org/10.21786/bbrc//15.1.22.
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The haphazard application of chemical fertilizers and pesticides causes tremendous damage to ecosystems and all biota. One of the most effective ways to tackle the threat is to use biofertilizer. Plant growth promoting bacteria (PGPB) are an important bacterial source for microbial fertilizers that can boost agricultural yields by encouraging plant growth. Bacterial isolates isolated from Saurashtra region, Gujarat, India were analysed for their capability to solubilize inorganic 'P' from tri calcium phosphate and production of indole acetic acid (IAA) quantitatively by bacterial. Production of ammonia, siderophore and hydrogen cyanide (HCN) by selected bacteria isolates was analysed. Biochemical characterization of selected bacterial isolates was done using Vitek 2 Compact system. Isolate GFS15C2 showed highest amount of phosphate solubilization, followed by isolate GFS07C1 and GFS01C1. Bacterial isolate GFS15C2 produced highest amount of IAA. All bacterial isolates were able produce ammonia. Eight bacteria isolates were be to produce HCN. Siderophore was produced by 14 bacterial isolates. In biochemical characterization all the bacterial isolates were able to use D-glucose. Based on biochemical characters clustering of bacteria isolates was done using Paleontological statistics software package for education and data analysis(PAST). Using cluster analysis by euclidean distance method based on biochemical characterization isolates GFS16C2 & SCS12C3 was found to have distinct characters than other isolates. The present study attempts to characterize PGPB which could be harnessed to improve plant growth. Several phosphate solubilizers and IAA producers also showed production of siderophores and HCN which suggests that these organisms do possess biocontrol ability. These PGPB microbial inoculants can be utilized to improve agricultural systems or as an alternate means of environmentally friendly plant disease biocontrol.
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Buerger, S., A. Spoering, E. Gavrish, C. Leslin, L. Ling, and S. S. Epstein. "Microbial Scout Hypothesis and Microbial Discovery." Applied and Environmental Microbiology 78, no. 9 (February 24, 2012): 3229–33. http://dx.doi.org/10.1128/aem.07308-11.
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ABSTRACTIn this study, we examine the temporal pattern of colony appearance during cultivation experiments, and whether this pattern could inform on optimizing the process of microbial discovery. In a series of long-term cultivation experiments, we observed an expected gradual increase over time of the total number of microbial isolates, culminating in a 700-fold colony count increase at 18 months. Conventional thought suggests that long-term incubations result in a culture collection enriched with species that are slow growing or rare, may be unavailable from short-term experiments, and likely are novel. However, after we examined the phylogenetic novelty of the isolates as a function of the time of their isolation, we found no correlation between the two. The probability of discovering either a new or rare species late in the incubation matched that of species isolated earlier. These outcomes are especially notable because of their generality: observations were essentially identical for marine and soil bacteria as well as for spore formers and non-spore formers. These findings are consistent with the idea of the stochastic awakening of dormant cells, thus lending support to the scout model. The process of microbial discovery is central to the study of environmental microorganisms and the human microbiome. While long-term incubation does not appear to increase the probability of discovering novel species, the technology enabling such incubations, i.e., single-cell cultivation, may still be the method of choice. While it does not necessarily allow more species to grow from a given inoculum, it minimizes the overall isolation effort and supplies needed.
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Clark, Chase M., Linh Nguyen, Van Cuong Pham, Laura M. Sanchez, and Brian T. Murphy. "Automated Microbial Library Generation Using the Bioinformatics Platform IDBac." Molecules 27, no. 7 (March 22, 2022): 2038. http://dx.doi.org/10.3390/molecules27072038.
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Libraries of microorganisms have served as a cornerstone of therapeutic drug discovery, though the continued re-isolation of known natural product chemical entities has remained a significant obstacle to discovery efforts. A major contributing factor to this redundancy is the duplication of bacterial taxa in a library, which can be mitigated through the use of a variety of DNA sequencing strategies and/or mass spectrometry-informed bioinformatics platforms so that the library is created with minimal phylogenetic, and thus minimal natural product overlap. IDBac is a MALDI-TOF mass spectrometry-based bioinformatics platform used to assess overlap within collections of environmental bacterial isolates. It allows environmental isolate redundancy to be reduced while considering both phylogeny and natural product production. However, manually selecting isolates for addition to a library during this process was time intensive and left to the researcher’s discretion. Here, we developed an algorithm that automates the prioritization of hundreds to thousands of environmental microorganisms in IDBac. The algorithm performs iterative reduction of natural product mass feature overlap within groups of isolates that share high homology of protein mass features. Employing this automation serves to minimize human bias and greatly increase efficiency in the microbial strain prioritization process.
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Milic, Jelena, Jelena Avdalovic, Tatjana Solevic-Knudsen, Gordana Gojgic-Cvijovic, Tanja Jednak, and Miroslav Vrvic. "Initial microbial degradation of polycyclic aromatic hydrocarbons." Chemical Industry and Chemical Engineering Quarterly 22, no. 3 (2016): 293–99. http://dx.doi.org/10.2298/ciceq150606043m.
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The group of polycyclic aromatic hydrocarbons (PAHs) are very hazardous environmental pollutants because of their mutagenic, carcinogenic and toxic effects on living systems. The aim of this study was to examine and compare the ability and efficiency of selected bacterial isolates obtained from oil-contaminated areas to biodegrade PAHs. The potential of the bacteria to biodegrade various aromatic hydrocarbons was assessed using the 2,6-dichlorophenol-indophenol assay. Further biodegradation of PAHs was monitored by gravimetric and gas-chromatographic analysis. Among the eight bacterial isolates, identified on the basis of 16S rDNA sequences, two isolates, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, had the ability to grow on and utilize almost all examined hydrocarbons. Those isolates were further examined for biodegradation of phenanthrene and pyrene, as single substrates, and as a mixture, in vitro for ten days. After three days, both isolates degraded a significant amount phenanthrene, which has a simpler chemical structure than pyrene. Planomicrobium sp.RNP01 commenced biodegradation of pyrene in the PAH mixture only after it had almost completly degraded phenanthrene. The isolated and characterized bacteria, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, have shown high bioremediation potential and are likely candidates to be used for degradation of highly toxic PAHs in contaminated areas.
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Vaish, S., N. Garg, and I. Z. Ahmad. "Bioprospecting of microbial isolates from biodynamic preparations for PGPR and biocontrol properties." Journal of Environmental Biology 42, no. 3 (May 4, 2021): 644–51. http://dx.doi.org/10.22438/jeb/42/3/mrn-1529.
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Aim: To study the role of microorganisms behind their bioenhancing, biocontrol properties, their enzymatic potential, and characterization of high performing microbial isolates on molecular basis. Methodology: Dominant culturable microbes including bacteria and fungi were isolated from biodynamic preparations and screened on the basis of plant-growth promoting (PGP) activities viz., ammonia production, phosphate solubilization, siderophore production, hydrogen cyanide production, seed germination efficacy and biocontrol properties. The dominant and effective microorganisms were screened for enzymes activities viz., pectinase, cellulase and amylase. The selected bacterial and fungal isolates, exhibiting higher enzyme activities, were subjected to molecular characterization. Results: Out of 68 bacterial and 25 fungal isolates from 8 biodynamic preparations (BD 500 - BD 507), 15 bacterial isolates exhibited high plant growth promoting activities while 10 bacterial isolates exhibited biocontrol activity against pathogens. Bacillus licheniformis isolated from BD 504, expressed high pectinase (2.595 U ml-1 min-1), cellulase (0.308 U ml-1 min-1) and amylase (0.418 U ml-1 min-1) activities. Fungal isolates with high enzymatic activities, were isolated from BD 500, 503 and 506, respectively. Interpretation: Microorganism isolates from biodynamic preparations possessed strong plant growth promoting, biocontrol and enzymatic properties, which might be responsible for the efficacy of organic preparations under field conditions.
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Munir, E., A. Lutfia, A. Hartanto, A. A. N. Fazri, C. Herdiyanti, R. Pratama, O. B. I. Sinaga, Z. A. Ramadani, and P. Hasanah. "Potential of landfill microbes in hydrocarbon degradation." IOP Conference Series: Earth and Environmental Science 977, no. 1 (June 1, 2022): 012095. http://dx.doi.org/10.1088/1755-1315/977/1/012095.
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Abstract Bioprospecting study of indigenous microbial community is still being approached especially for some microbial strains in bioremediation aspect. The objective of this study was to obtain a collection of indigenous bacterial and fungal isolates capable of growing on hydrocarbon medium. Soil samples were collected from Terjun Landfill located in Medan City, North Sumatra. Mineral salt agar medium supplemented with 10% (v/v) of hydrocarbon sources (diesel oil, pesticide, dye wastewater) was used to screen the microbial capacity in utilizing the hydrocarbon compounds as sole carbon source. Each isolate was categorized based on their growth performance or colony diameter on agar medium namely none (-), slow (+), moderate (++), and rapid (+++). The results obtained a total of 27 bacterial isolates and 6 fungal isolates. Majority of microbial isolates showed a better growth on pesticide medium than in diesel oil and dye wastewater which indicated their capability in converting the pesticide as nutrient. It is worthwhile to investigate the further application of our indigenous strains in contaminated areas in order to ensure the environmental sustainability.
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Kumari, Kiran, R. C. Aanad, and Neeru Narula. "Microbial degradation of polyethylene (PE)." South Pacific Journal of Natural and Applied Sciences 27, no. 1 (2009): 66. http://dx.doi.org/10.1071/sp09012.
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Thirty two bacterial isolates were obtained from soil by soil burial method followed by enrichment culture technique in film culturing (FC) media. Bacterial isolates differing in morphology were selected, purified and maintained at 4°C. Thirty % of these isolates were found to be Gram negative and 50% showed positive starch hydrolysis test and were screened for their ability to degrade Low Density Polyethylene (untreated, UV and heat strips) in film culturing media and percent weight loss of polyethylene after 4th week was determined. Among various isolates, highest degradation was by Is 3, Is 22 and Is 31 to the range of 25–27%, of UV treated polyethylene strips. High temperature (40°C), was found to enhance degradation rate of polyethylene more effectively by 24–28% compared to low temperature at 30°C (18–21%). Degradation of treated polyethylene strips (UV, heat steam) was up to 4% by compost treatment as studied by using CO2 evolution, an estimation tool to analyze % degradation. Bacterial activity was also affected by various environmental factors like sunlight, temperature, oxygen etc.
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Munir, Neelma, Kanza Malik, Rasheeda Bashir, Imran Altaf, and Shagufta Naz. "Isolation and Characterization of Polysaccharide- Degrading Microbes from Compost Samples." Journal of Environmental Science and Management 24, no. 2 (December 31, 2021): 10–16. http://dx.doi.org/10.47125/jesam/2021_2/02.
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This study isolated and identified novel microbial polysaccharide degrading species involved in composting process. Bacteria were isolated from different composting samples from Lahore Compost Private Limited, Lahore, Pakistan. Physico-chemical analysis of compost samples made from municipal waste were collected at 15-day interval during the composting process. A total of 55 bacteria were isolated and identified using morphological and biochemical characteristics. Out of 55 isolates, 26 mesophilic and 14 thermophilic had cellulose degrading potential. Out of 26 mesophyllic bacterial isolates, 20 were found cellulolytic. The isolated bacteria and fungi were identified morphologically and biochemically. The highest potential to degrade cellulose was recorded from four Bacillus strains. Molecular characterization of potential cellulolytic bacteria by 16S rRNA was performed. Bacillus sp. were found as the most prevalent cellulolytic bacteria in composting process. Fungi were also isolated and characterized morphologically and microscopically following techniques. The potential cellulolytic fungal isolates were Aspergillus fumigatus, Mucor sp., Saccharomyces sp., and Aspergillus niger. The results of this study would be helpful in highlighting the potential role of different microbes involved in enhancement of the composting process. These microbes can be used for the preparation of microbial inoculum based on their polysaccharide cellulose, bacteria, composting degrading ability.
Dissertations / Theses on the topic "Environmental microbial isolates"
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Nicholas, Rebecca Charlotte. "The effect of ozone and open air factor on environmental microbial isolates of significance in the food industry." Thesis, Cardiff Metropolitan University, 2014. http://hdl.handle.net/10369/7557.
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Ozone and open air factor (OAF) have been reported in the research literature as being effective anti-microbial agents. The action of ozone on microbes is relatively well understood, but the action of OAF is not well characterised. Both ozone and OAF have relatively short half-lives and do not leave behind toxic residues. The advantages of using a gas instead of aerosols, or droplets produced by fogging to decontaminate surfaces, is that a gas will come into contact with the horizontal, vertical and inverted planes of the surfaces (by diffusion), whereas aerosols or droplets will be affected by gravity. The effect of gaseous ozone, OAF and ozonated water with or without d-limonene emulsified in alcohol on surface attached and biofilm environmental L. monocytogenes amd P. aeruginosa were investigated. The interaction of each treatment with microorganisms was elucidated by determining microbial survival on different food contact surfaces, detecting cell injury by examining treated environmental L. monocytogenes cells using scanning electron and atomic force microscopy. All treatments were significantly more effective in eliminating the gram negative than the gram positive bacteria. This may be due to differences in cell wall structure and the cell’s ability to produce extracellular polymeric substances. Scanning electron microscopy revealed that gaseous ozone caused the cells to bleb out their cellular contents, whereas for OAF treated cells, holes were apparent in the cell wall. The ozonated water treatments were more effective in stripping the biofilm away from the surface substrata. The atomic force microscope showed that OAF, ozonated water and terpene, and the terpene in water treatments caused visible cell surface property changes, compared to gaseous ozone and ozonated water treatment alone.
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Greene, Elizabeth Anne. "Microbial sulfolane degradation by environmental organisms isolated from contaminated sour gas plant sediments." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0034/NQ46841.pdf.
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Giddey, Kirsten Francis. "Investigating the efficacy of hydrogen peroxide agaisnt isolated environmental Escherichia coli strains." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96613.
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Thesis (MSc Food Sc)--Stellenbosch University, 2015.ENGLISH ABSTRACT: Surface water used for irrigation is often highly contaminated on a microbial level. Using contaminated surface water for the irrigation of fresh produce can lead to foodborne disease outbreaks and Escherichia coli has been a major cause of foodborne outbreaks associated with fresh produce over the past few years. There are many possible on-farm treatment options available to decrease the high microbial loads present in surface water, one of these is H2O2 and various factors can influence its use. The aim of this study was to determine the efficacy of H2O2 on different E. coli strains. Water from the Plankenburg River was sampled and treated with (250, 300 and 350 mg.L-1) H2O2 and the impact at 0, 30, 60, 90 and 120 min was then evaluated. It was found that the log reductions differed between samples. Log reductions ranged between 1.60 – 2.63 for Aerobic colony counts (ACC), total coliforms and Escherichia coli. The water was not considered safe for irrigation use although it had been treated with H2O2. Reference (ATCC) and environmental E. coli strains were individually treated with H2O2 (250, 300 and 350 mg.L-1) at 0, 30, 60, 90 and 120 min. Log reductions for the ATCC strains ranged between 2.13 – 5.48. This indicated a variation in H2O2 resistance between the different reference strains tested. Log reductions for the environmental E. coli strains ranged between 2.17 – 3.93. Escherichia coli M53 and MJ56 were the most resistant and most sensitive environmental strains to the H2O2 treatment, respectively. Once again it was observed that variations existed between the log reductions achieved for different strains. Overall, it was observed that the ATCC E. coli strains were more sensitive to the H2O2 treatments when compared the environmental strains. This indicates that ATCC strains should not be used for H2O2 treatment optimisation. Certain factors can influence the efficacy of H2O2 such as concentration and organic matter (chemical oxygen demand) present in the water. Different H2O2 concentrations were evaluated (50, 350, 700 and 1 000 mg.L-1) on two E. coli strains (M53 and W1371). Results indicated that 50 mg.L-1 was not effective as less than 1 log reduction was achieved after 120 min. When 350 and 700 mg.L-1 were used similar log reductions were achieved (1.78 – 2.27), which was not expected. Using 1 000 mg.L-1 was considered an effective concentration that resulted in no growth present after 120 min. Escherichia coli strain W1371 carried EPEC virulence factors (potential pathogen). This was included in the study in order to determine how a strain carrying virulence factors would react to H2O2. Escherichia coli W1371 was considered resistant to the H2O2 treatment and log reductions were similar to that achieved for M53. The catalase activity of the E. coli strains was studied to determine if a link existed between catalase activity and H2O2 resistance. Although a trend was observed between heat-stable catalase activity and H2O2 resistance, there were exceptions. It was concluded that high catalase activity does not always coincide with H2O2 resistance and that other mechanisms might also contribute to E. coli survival. Overall, it was observed that there are certain factors that influence the efficacy of H2O2 as a treatment option. It can be concluded that environmental E. coli strains are generally more resistant to the H2O2 treatment compared to ATCC E. coli strains, this needs to be considered when using H2O2 or other chemical disinfectants as a treatment option.
AFRIKAANSE OPSOMMING: Oppervlakwater wat gebruik word vir besproeiing is dikwels op ‘n mikrobiese vlak hoogs gekontamineer. Die gebruik van oppervlakwater vir die besproeiing van vars produkte kan tot die uitbraak van voedselgedraagde siektes lei. Escherichia coli was een van die hoofoorsake van voedselgedraagde uitbrake geassosieerd met vars produkte gedurende die laaste paar jaar. Daar is verskeie moontlike behandelingsmetodes op plaasvlak beskikbaar om die hoë mikrobiese las in oppervlakwater te verlaag. Een hiervan is waterstofperoksied (H2O2) en verskeie faktore kan die gebruik hiervan beïnvloed. Die doel van hierdie studie was om die doeltreffendheid van H2O2 op verskillende E. coli isolate te bepaal. Watermonsters uit die Plankenburg Rivier is behandel met drie konsentrasies H2O2 (250, 300 en 350 mg.L-1) en die impak is na 0, 30, 60, 90 en 120 minute geëvalueer. Daar is gevind dat die log reduksies tussen monsters verskil het. Log reduksies het gewissel tussen 1.60 en 2.63 vir aerobiese kolonietellings (AKT), totale kolivorme en E. coli. Selfs na H2O2 behandeling, is die water nie as veilig vir besproeiing beskou nie. Verwysingsisolate (ATCC) en omgewingsisolate van E. coli is afsonderlik met H2O2 behandel (250, 300 en 350 mg.L-1) vir 0, 30, 60, 90 en 120 minute. Log reduksies vir die ATCC isolate het gewissel tussen 2.13 en 5.48. Hierdie verskille dui op die variasies wat tussen die getoetste verwysingsisolate voorkom. Log reduksies vir die omgewingsisolate het gewissel tussen 2.17 en 3.93. Escherichia coli M53 en MJ56 was onderskeidelik die mees weerstandbiedende en mees sensitiewe verwysingsisolate wat getoets is. Verskille in log reduksies het daarop gedui dat isolaat variasies voorkom. In geheel is dit gevind dat die ATCC E. coli isolate meer sensitief was vir die H2O2 behandelings vergeleke met die omgewingsisolate. Dit toon dat die ATCC isolate nie gebruik moet word vir H2O2 behandeling optimering nie. Sekere faktore, soos die konsentrasie en organiese materiaal (chemiese suurstof vereiste) in die water, kan die doeltreffendheid van H2O2 behandeling beïnvloed. Verskillende H2O2 konsentrasies is geëvalueer (50, 350, 700 en 1000 mg.L-1) op twee E. coli isolate (M53 en W1371). Resultate dui daarop dat 50 mg.L-1 nie effektief was nie omdat minder as 1 log reduksie behaal is na 120 minute. Toe 350 en 700 mg.L-1 gebruik is, is soortgelyke log reduksies (1.78 – 2.27) teen verwagting in behaal. Die gebruik van 1000 mg.L-1 is as ‘n effektiewe behandeling beskou aangesien daar geen groei na 120 minute teenwoordig was nie. Escherichia coli isolaat W1371 besit EPEC virulensie faktore (potensiële patogeen). Dit is in die studie ingesluit ten einde te bepaal hoe ‘n isolaat met virulensie faktore sou reageer op H2O2. Escherichia coli W1371 is as weerstandbiedend teen die H2O2 behandeling beskou en log reduksies was soortgelyk aan die van M53 . Die katalase aktiwiteit van die E. coli isolate is bestudeer om te bepaal of ʼn skakel bestaan tussen katalase aktiwiteit en H2O2 weerstandbiedendheid. Alhoewel ‘n tendens waargeneem is tussen hitte-stabiele katalase aktiwiteit en H2O2 weerstandbiedendheid, was daar uitsonderings. Die gevolgtrekking was dat hoë katalase aktiwiteit nie altyd saamval met H2O2 weerstandbiedendheid nie en dat ander meganismes ook mag bydra tot E. coli oorlewing. In geheel is dit waargeneem dat daar sekere faktore is wat die doeltreffendheid van H2O2 as ‘n behandelingsmetode beïnvloed. Daar is gevind dat omgewingsisolate van E. coli in die algemeen meer weerstandbiedend is teenoor H2O2 behandeling in vergelyking met ATCC E. coli isolate. Dit moet in ag geneem word wanneer H2O2 of ander chemiese ontsmettingsmiddels oorweeg word as ʼn behandelingsopsie.
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Morelos, M., Phillip R. Scheuerman, and G. Gist. "Antibiotic Resistant Bacteria Isolated from German Cockroaches Collected from a Hospital Laundry Facility." Digital Commons @ East Tennessee State University, 1989. https://dc.etsu.edu/etsu-works/2887.
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Shah, Siddharth Prakashchandra. "LIPID COMPOSITIONS OF MICROBIAL ORGANISMS ISOLATED FROM EXTREME ENVIRONMENTS AND THEIR IMPLICATION IN THERMO STABILITY OF BACTERIAL CELL MEMBRANE STRUCTURE." Diss., Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/419298.
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ChemistryPh.D.
Microorganisms with an ability to thrive in harsh environments are referred as “extremophiles”. With advances in biotechnology, interest has grown in the extremophile research because of their unique macromolecules’ characteristics due to their growth environments. Over last decade, researchers have isolated many extremophiles from environments like volcano, salt lakes, hydrothermal vents, deep oceans, Antarctica glaciers etc. Macromolecules of these extremophiles are responsible for their survival in extreme environments. In this research work we have isolated lipid molecules from three different microorganisms. 1) GWE1 strain, a thermophilic bacterium, isolated from dark crusty material from sterilization ovens. 2) 7L strain, a thermophilic bacterium, isolated from Chilean Copahue Volcano. 3) I1P strain, a facultative anaerobe of the family Enterobacteriaceae, recently isolated from Antarctica. Complex lipid arrangement and/or type in the cell membrane are known to affect thermostability of microorganisms and efforts were made to understand the chemical nature of the polar lipids of membrane. In this work, we extracted total lipids from cell membrane, separated them by TLC into various fractions and characterize the lipid structures of fractions with analytical tools such as 1H, 13C, 31P and 2D NMR spectroscopy, ATR-FTIR spectroscopy and MSn spectrometry. In GWE1 strain, we were able to identify glycerophosphoethanolamine, glycerophosphate, glycerophosphoglycerol and cardiolipin lipid classes and an unknown glycerophospholipid class with novel MS/MS spectra pattern. We have also noticed the presence of saturated iso-branched fatty acids with NMR spectra in individual lipid classes. In case of I1P strain, we have identified glycerophosphoglycerol, glycerophosphoethanolamine, glycerophosphate, and acyl glycerophosphoglycerol lipid classes with unsaturated fatty acids in their structure, which could be one of the many reasons for survivability at lower temperatures. In case of 7L strain, we were able to identify glycerophosphoglycerol, cardiolipin, glycerophosphoethanolamine and glycerophosphate lipid classes with saturated iso branched fatty acids. FAME analysis revealed iso-15:0 (52.29 %) and iso-17:0 (18.64 %) as major fatty acyl chains. We did not observe major difference in polar head group composition of lipid classes between thermophiles (GWE1 and 7L) compare to psychrophiles (I1P). Major difference among these three strains was in fatty acid composition of lipid molecule. Both thermophiles showed presence of lipids with long chain saturated fatty acids while I1P showed presence of lipid molecule with unsaturated fatty acid chain. Lipids made of unsaturated fatty acids have lower melting points and they introduce kink in the cell membrane structure. At lower temperatures, these effects allow membrane to maintain fluidity and its functionality, which in turn allows the microorganism to grow at lower temperature. Lipids made with saturated iso branched fatty acid chain have higher melting points and they pack together densely in cell membrane. At high temperature because of higher melting point and dense packing, membrane fluidity is not affected and this effect allows microorganism to grow at the higher temperature. We believe that change in fatty acid composition is one of the many reasons for these microorganisms to survive the extreme condition. Thermostability of the other macromolecules (DNA, enzyme) of these extremophiles is not studied in this dissertation.
Temple University--Theses
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Dumontet, Stefano. "Marine aquatic environment as a source of potential human pathogens : studies on prevalence, ecology and characterisation of Aeromonas spp. and Vibrio vulnificus isolated from marine environment /." Uppsala : Dept. of Veterinary Microbiology, Swedish Univ. of Agricultural Sciences ([Institutionen för veterinärmedicinsk mikrobiologi], Sveriges lantbruksuniv.), 2002. http://epsilon.slu.se/avh/2002/91-576-6370-X.pdf.
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Sislak, Christine Demko. "Novel Thermophilic Bacteria Isolated from Marine Hydrothermal Vents." PDXScholar, 2013. https://pdxscholar.library.pdx.edu/open_access_etds/1486.
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As part of a large study aimed at searching for patterns of diversity in the genus Persephonella along the north to south geochemical gradient of the ELSC, ten novel strains of Alphaproteobacteria were isolated unexpectedly. Using defined media under microaerophilic conditions to enrich for Persephonella from chimney samples collected at the seven vent fields on the ELSC and the dilution to extinction by serial dilution method to purify cultures, a total of ten strains belonging to the Alphaproteobacteria were isolated. Two of these isolates, designate MN-5 and TC-2 were chosen for further characterization and are proposed as two new species of a novel genus to be namedThermopetrobacter. Both strains are aerobic, capable of chemoautotrophic growth on hydrogen and grow best at 55°C, pH 6 and 3.0% NaCl. Strain MN-5 is capable of heterotrophic growth on pyruvate and malate and TC-2 is only able to grow heterotrophically with pyruvate. The GC content of MN-5 is 69.1 and TC-2 is 67 mol%. GenBank BLAST results from the 16S rRNA gene reveal the most closely related sequence to MN-5 is 90% similar and the most closely related sequence to strain TC-2 is 89% similar.
Sampling at a shallow marine vent on the coast of Vulcano Island, Italy in 2007 led to the isolation of a novel species of Hydrogenothermus, a genus within the Hydrogenothermaceae family. This isolate, designated NV1, represents the secondHydrogenothermusisolated from a shallow marine vent. NV1 cells are rod-shaped, approximately 1.5μm long and 0.7μm wide, motile by means of a polar flagellum and grow singularly or in short chains. Cells grow chemoautotrophically using hydrogen or thiosulfate as electron donors and oxygen as the sole electron acceptor. Growth was observed between 45 and 75°C with an optimum of 65°C (doubling time 140 min), pH 4.0-6.5 and requires NaCl (0.5-6.0% w/v). The G+C content of total DNA is 32 mol%.
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Vickers, Mark. "Bacteria isolated from orthopaedic patients in north-eastern Australia: Epidemiology, multi-drug resistance and the impact of environment and season." Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/213222/1/Mark_Vickers_Thesis.pdf.
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In orthopaedic medicine, post-operative infections are associated with increased morbidity, mortality and higher health care costs. There exists a need to improve understanding of factors influencing infection rates and antimicrobial resistance. Particularly for orthopaedic surgery performed in hotter, wetter climates where the tropical microbial niche presents unique challenges. This clinical research analyses the risks of orthopaedic post-operative infections associated with weather and climatic variables. Detailed epidemiological data is presented which describes features of bacteria complicating orthopaedic surgery in North Eastern Australia and the relationship between multi-drug resistance, geographic location, season and weather. Antimicrobial prescribing recommendations are also provided.
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"Integrated -omic study of deep-sea microbial community and new Pseudoalteromonas isolate." Doctoral diss., 2013. http://hdl.handle.net/2286/R.I.21027.
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abstract: This thesis research focuses on phylogenetic and functional studies of microbial communities in deep-sea water, an untapped reservoir of high metabolic and genetic diversity of microorganisms. The presence of photosynthetic cyanobacteria and diatoms is an interesting and unexpected discovery during a 16S ribosomal rRNA-based community structure analyses for microbial communities in the deep-sea water of the Pacific Ocean. Both RT-PCR and qRT-PCR approaches were employed to detect expression of the genes involved in photosynthesis of photoautotrophic organisms. Positive results were obtained and further proved the functional activity of these detected photosynthetic microbes in the deep-sea. Metagenomic and metatranscriptomic data was obtained, integrated, and analyzed from deep-sea microbial communities, including both prokaryotes and eukaryotes, from four different deep-sea sites ranging from the mesopelagic to the pelagic ocean. The RNA/DNA ratio was employed as an index to show the strength of metabolic activity of deep-sea microbes. These taxonomic and functional analyses of deep-sea microbial communities revealed a `defensive' life style of microbial communities living in the deep-sea water. Pseudoalteromonas sp.WG07 was subjected to transcriptomic analysis by application of RNA-Seq technology through the transcriptomic annotation using the genomes of closely related surface-water strain Pseudoalteromonas haloplanktis TAC125 and sediment strain Pseudoalteromonas sp. SM9913. The transcriptome survey and related functional analysis of WG07 revealed unique features different from TAC125 and SM9913 and provided clues as to how it adapted to its environmental niche. Also, a comparative transcriptomic analysis of WG07 revealed transcriptome changes between its exponential and stationary growing phases.Dissertation/Thesis
Ph.D. Civil and Environmental Engineering 2013
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Hsu, Shi-Fang, and 徐仕芳. "Effect of toluene addition on the survival of fluorescent Pseudomonas sp. isolate F5 in soil and the changes of microbial compositions in its surrounding environment." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/48416893656668770947.
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碩士東吳大學
微生物學系
85
How to study the influence of stress on the composition of surrounding microbial community has being a challenging topic for the researchers. In thisstudy, we have selected fluorescent Pseudomonas sp. F5 which can use toluene asa sole carbon source, as the targeting tool. This bacterium was identified byusing API 20NE, Biolog GN MicroPlate, and Microbial Identification System andthe results indicate that it is P. putida, P. fluorescence , and P. putida and(or) P. fluorescence, respectively. Since this bacterium can degrade toluene,based on the information of xylE and xylS nucleic acid sequences, we havedesigned 4 pairs of primer to be used in PCR amplification studies. The resultsindicate that only when using xyls2xylsn1 as primers, and KlenTaq as thepolymerase that we have obtained an amplified product with maching our design. As for the enumeration studies, besides using antibiotic amended selectivemedia to follow the changes in F5 population, we usen 1/2 strength TSA and soilextract agar to enumerate the total soil bacteria numbers. Although the colonynumbers developed on both media were around 107 - 108 CFU/g of freshed soil buttheir morphologies were very different. The colonies developed on soil extractagar were mainly actinomycetes. Through bi-cultural studies, we failed todetect any relationship between the decilne in numbers of isolate F5 and theantagonistic activities of theseactinomycetes. Reverse Sample Genome Probing (RSGP) was used to follow the cnges in microbial communities. DNA from soilsamples with different treatment, taken at different times were extracted, andlabelled by using GI-non-isotope labelling system. These labelled DNA were thenhybridized with a master membrane spotted with 16 standard bacterial isolates.It is clear from the resul the composition of bacterial communities changed withtime of incubation. At the same time, we also observed the influence of the addation of toluene to the soil bacterial communities. Based on the theoreticalDNA content of a single bacteriam cell, it is clear that the numbers derived ishigher than the numbers obtained from the selective media. Furthermore, thebacteria isolated from the 1/2 strength TSA plate were further identified usingMIS identification system. However, the results indicate a high percentage of"no match" which suggests that the data bank of this system needs to be improvedbefore this method can be empolyed. The method of RSGP can effectively outline the qualitative changes in various bacterial populations in the test emvironment. However, before quantitative changes can be monitored, a lot of basic informationstill need to be established
Books on the topic "Environmental microbial isolates"
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Kirchman, David L. Genomes and meta-omics for microbes. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0005.
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The sequencing of entire genomes of microbes grown in pure cultures is now routine. The sequence data from cultivated microbes have provided insights into these microbes and their uncultivated relatives. Sequencing studies have found that bacterial genomes range from 0.18 Mb (intracellular symbiont) to 13 Mb (a soil bacterium), whereas genomes of eukaryotes are much bigger. Genomes from eukaryotes and prokaryotes are organized quite differently. While bacteria and their small genomes often grow faster than eukaryotes, there is no correlation between genome size and growth rates among the bacteria examined so far. Genomic studies have also highlighted the importance of genes exchanged (“horizontal gene transfer”) between organisms, seemingly unrelated, as defined by rRNA gene sequences. Microbial ecologists use metagenomics to sequence all microbes in a community. This approach has revealed unsuspected physiological processes in microbes, such as the occurrence of a light-driven proton pump, rhodopsin, in bacteria (dubbed proteorhodopsin). Genomes from single cells isolated by flow cytometry have also provided insights about the ecophysiology of both bacteria and protists. Oligotrophic bacteria have streamlined genomes, which are usually small but with a high fraction of genomic material devoted to protein-encoding genes, and few transcriptional control mechanisms. The study of all transcripts from a natural community, metatranscriptomics, has been informative about the response of eukaryotes as well as bacteria to changing environmental conditions.
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Gleń-Karolczyk, Katarzyna. Zabiegi ochronne kształtujące plonowanie zdrowotność oraz różnorodność mikroorganizmów związanych z czernieniem pierścieniowym korzeni chrzanu (Atmoracia rusticana Gaertn.). Publishing House of the University of Agriculture in Krakow, 2019. http://dx.doi.org/10.15576/978-83-66602-39-7.
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Horseradish roots, due to the content of many valuable nutrients and substances with healing and pro-health properties, are used more and more in medicine, food industry and cosmetics. In Poland, the cultivation of horseradish is considered minor crops. In addition, its limited size causes horseradish producers to encounter a number of unresolved agrotechnical problems. Infectious diseases developing on the leaves and roots during the long growing season reduce the size and quality of root crops. The small range of protection products intended for use in the cultivation of horseradish generates further serious environmental problems (immunization of pathogens, low effectiveness, deterioration of the quality of raw materials intended for industry, destruction of beneficial organisms and biodiversity). In order to meet the problems encountered by horseradish producers and taking into account the lack of data on: yielding, occurrence of infectious diseases and the possibility of combating them with methods alternative to chemical ones in the years 2012–2015, rigorous experiments have been carried out. The paper compares the impact of chemical protection and its reduced variants with biological protection on: total yield of horseradish roots and its structure. The intensification of infectious diseases on horseradish leaves and roots was analyzed extensively. Correlations were examined between individual disease entities and total yield and separated root fractions. A very important and innovative part of the work was to learn about the microbial communities involved in the epidemiology of Verticillium wilt of horseradish roots. The effect was examined of treatment of horseradish cuttings with a biological preparation (Pythium oligandrum), a chemical preparation (thiophanate-methyl), and the Kelpak SL biostimulator (auxins and cytokinins from the Ecklonia maxima algae) on the quantitative and qualitative changes occurring in the communities of these microorganisms. The affiliation of species to groups of frequencies was arranged hierarchically, and the biodiversity of these communities was expressed by the following indicators: Simpson index, Shannon–Wiener index, Shannon evenness index and species richness index. Correlations were assessed between the number of communities, indicators of their biodiversity and intensification of Verticillium wilt of horseradish roots. It was shown that the total yield of horseradish roots was on average 126 dt · ha–1. Within its structure, the main root was 56%, whereas the fraction of lateral roots (cuttings) with a length of more than 20 cm accounted for 26%, and those shorter than 20 cm for 12%, with unprofitable yield (waste) of 6%. In the years with higher humidity, the total root yield was higher than in the dry seasons by around 51 dt · ha–1 on average. On the other hand, the applied protection treatments significantly increased the total yield of horseradish roots from 4,6 to 45,3 dt · ha–1 and the share of fractions of more than 30 cm therein. Higher yielding effects were obtained in variants with a reduced amount of foliar application of fungicides at the expense of introducing biopreparations and biostimulators (R1, R2, R3) and in chemical protection (Ch) than in biological protection (B1, B2) and with the limitation of treatments only to the treatment of cuttings. The largest increments can be expected after treating the seedlings with Topsin M 500 SC and spraying the leaves: 1 × Amistar Opti 480 SC, 1 × Polyversum WP, 1 × Timorex Gold 24 EC and three times with biostimulators (2 × Kelpak SL + 1 × Tytanit). In the perspective of the increasing water deficit, among the biological protection methods, the (B2) variant with the treatment of seedlings with auxins and cytokinins contained in the E. maxima algae extract is more recommended than (B1) involving the use of P. oligandrum spores. White rust was the biggest threat on horseradish plantations, whereas the following occurred to a lesser extent: Phoma leaf spot, Cylindrosporium disease, Alternaria black spot and Verticillium wilt. In turn, on the surface of the roots it was dry root rot and inside – Verticillium wilt of horseradish roots. The best health of the leaves and roots was ensured by full chemical protection (cuttings treatment + 6 foliar applications). A similar effect of protection against Albugo candida and Pyrenopeziza brassicae was achieved in the case of reduced chemical protection to one foliar treatment with synthetic fungicide, two treatments with biological preparations (Polyversum WP and Timorex Gold 24 EC) and three treatments with biostimulators (2 × Kelpak SL, 1 × Tytanit). On the other hand, the level of limitation of root diseases comparable with chemical protection was ensured by its reduced variants R3 and R2, and in the case of dry root rot, also both variants of biological protection. In the dry years, over 60% of the roots showed symptoms of Verticillium wilt, and its main culprits are Verticillium dahliae (37.4%), Globisporangium irregulare (7.2%), Ilyonectria destructans (7.0%), Fusarium acuminatum (6.7%), Rhizoctonia solani (6.0%), Epicoccum nigrum (5.4%), Alternaria brassicae (5.17%). The Kelpak SL biostimulator and the Polyversum WP biological preparation contributed to the increased biodiversity of microbial communities associated with Verticillium wilt of horseradish roots. In turn, along with its increase, the intensification of the disease symptoms decreased. There was a significant correlation between the richness of species in the communities of microbial isolates and the intensification of Verticillium wilt of horseradish roots. Each additional species of microorganism contributed to the reduction of disease intensification by 1,19%.
Book chapters on the topic "Environmental microbial isolates"
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Belechheb, Taoufik, Mohammed Bakkali, Amin Laglaoui, and Abdelhay Arakrak. "Characterization and Efficiency of Rhizobial Isolates Nodulating Cytisus monspessulanus in the Northwest of Morocco: In Relation to Environmental Stresses." In Environmental and Microbial Biotechnology, 63–72. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2576-6_3.
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Sowmiya Sri, S., V. Suji, and G. Sophia Reena. "Microbiome Analysis of Pesticide Contaminated Soils Its Impact on the Microbial Isolates." In Environmental Science and Engineering, 35–40. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-64122-1_3.
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Somova, Lydia A., Nickolay S. Pechurkin, Mark Nelson, and Lawrence K. Wang. "Microbial Ecology of Isolated Life Support Systems." In Environmental Biotechnology, 257–86. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-140-0_6.
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Hamane, Samia, Mounir Hassani Zerrouk, Anass E. Lyemlahi, Saida Aarab, Amin Laglaoui, Mohammed Bakkali, and Abdelhay Arakrak. "Screening and Characterization of Phosphate-Solubilizing Rhizobia Isolated from Hedysarum pallidum in the Northeast of Morocco." In Environmental and Microbial Biotechnology, 113–24. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2576-6_7.
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Tsuruta, Takehiko. "Biosorption of Uranium for Environmental Applications Using Bacteria Isolated from the Uranium Deposits." In Microbes and Microbial Technology, 267–81. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7931-5_11.
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Panosyan, Hovik. "Thermophilic and Halophilic Prokaryotes Isolated from Extreme Environments of Armenia and Their Biotechnological Potential." In Microbial Versatility in Varied Environments, 13–34. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3028-9_2.
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Guimarães, Tábata Cristina, Thiara Santana Gomes, Clara Dourado Fernandes, Fernanda Dantas Barros, Kamila Valença Oliveira, Muhammad Bilal, Ram Naresh Bharagava, Luiz Fernando Romanholo Ferreira, and Luciana M. Hollanda. "Antitumor Microbial Products by Actinomycetes Isolated from Different Environments." In Microorganisms for Sustainability, 113–60. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2679-4_5.
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Fle, Manfred G., Jan L. W. Rademaker, Frank J. Louws, James Versalovic, and Frans J. De Bruijn. "Section 3 update: Genotyping of bacterial isolates from the environment using Low-Molecular-Weight RNA fingerprints." In Molecular Microbial Ecology Manual, 2489–512. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_305.
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Swathi Priya, S., C. Shanmuga Priya, and J. Maria Shyla. "Analysis of Microbial Isolate from Contaminated Street Food with a Potential to Degrade Food Waste." In Environmental Science and Engineering, 235–42. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-64122-1_16.
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Sikdar, Debosmita, Ivy Kanungo, and Dipanwita Das. "Microbial Enzymes: A Summary Focusing on Biotechnology Prospective for Combating Industrial Pollutants." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), 70–76. Dordrecht: Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_8.
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AbstractEnvironmental issues are growing at an alarming rate and addressing the same is the need of the hour. Hazardous industrial pollutants and discharges are adding to the misery. Therefore, new ideas and technologies are being created and adopted to deal with ever increasing conservational troubles. Due to the burning issue of environmental pollution rising daily, a paradigm shift towards more sustainable and greener has to be pondered on. Microbial enzymes are such versatile, useful and beneficial weapons those can be exploited to combat the above-mentioned issues. In this aspect various works have been done and different sources of isolation of microbes and their fermentation process for procuring enzymes from them have been investigated in detail in those work. Pualsa Jagdish et al.’s work (2013) from Viva College, Virar, and Maharashtra entails that Lipase enzyme was procured from curd and waste oil was used as substrate. Lipase was produced by Lactobacillus sp. Whose lipolytic activity was calculated to be 0.082 U/mg. This enzyme if isolated under favorable conditions can be used to be applied for various industrial purposes in order to suppress the pollution rate and reduced the dependency on market-based chemicals and reagents those are highly dangerous and harmful. Work of Ashutosh Nema et al. (2019) [1], talks about the use of lipase enzyme as well as proteases are used as catalysts in biodiesel production as an effective and economical approach. According to Wu et al., large scale productions of protease have been achieved from Aspergillus species for their application in food and beverage industries. Alkaline proteases were reported to be produced under solid state fermentation processes by A. flavus and A. oryzae. Ikram-Ul-Haq and Mukhtar (2015) [2] stated that Penicillium sp. Alkaline proteases were generated under both solid state and submerged fermentation. The Mucor sp. of fungi can produce protease for milk clotting and can substitute rennin in the cheese making industry. Fungal enzymes are commonly used in industries over bacterial enzymes due to various technical reasons such as the feasibility of gaining enzymes at a high concentration in the fermentation medium and easier downstream processing. This way it can be encapsulated that microbial enzymes are savior in the field of pollution remediation and replacer of harsh and hazardous chemicals for carrying out various industrial applications.
Conference papers on the topic "Environmental microbial isolates"
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Taura, Usman, Sara Al-Araimi, Saif Al-Bahry, Yahya Al-Wahaibi, and Lujain Al-Rashdi. "Isolation of Autochthonous Consortium for the Bioremediation of Oil Contaminated Produced Water." In SPE Nigeria Annual International Conference and Exhibition. SPE, 2022. http://dx.doi.org/10.2118/212024-ms.
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Abstract In this research, we isolated indigenous bacteria capable of remediating oil-contaminated produced water in an efficient, cost-effective and environmentally friendly manner. Nine different produced water samples were collected from Omani oil fields and analysed for their physicochemical properties and microbial communities present. Different technologies were performed to extract the DNA of the microbial community cultured in different media. Metagenomic classification of the microbial community showed that the abundant genera are the Acidithiobacillus, Proteinphilum and Marinobacter. The isolated microbes that showed the highest efficiency in oil degradation were further evaluated for liquid-based biodegradation as well as in naturally occurring and artificially contaminated soil. Fourteen bacteria samples were found to be efficient in bioremediating the three environments tested. In the liquid-based media, the isolates were able to degrade the heavy oil carbon chains (C14-C20) by at least 50% after 1 week period, while some of the most potent isolates have achieved more than 95% or completely degraded all the hydrocarbon chains. Similarly, in the naturally contaminated soil, the isolates demonstrated a complete degradation of the lighter carbon molecules from C10-C16 and also achieved a higher than 90% degradation for the heavier components. Likewise, the isolates have exhibited similar biodegradation ability when exposed to an induced contaminated soil where all the lower carbon chains (C12-C17) were mostly degraded by the microbes in the samples.
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Rastimesina, Inna, Olga Postolachi, Valentina Vorona, Vera Mamaliga, and Alina Voinescu. "IDENTIFICATION OF MIXED MICROBIAL CONSORTIA ISOLATED FROM POLYETHYLENE FILMS SURFACE." In International Symposium "The Environment and the Industry". National Research and Development institute for Industrial Ecology, 2022. http://dx.doi.org/10.21698/simi.2022.ab35.
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Chou, Fong-In, Chia-Chin Li, Tzung-Yuang Chen, and Hsiao-Wei Wen. "Microbial Occurrence in Bentonite-Based Buffer Materials of a Final Disposal Site for Low Level Radioactive Waste in Taiwan." In ASME 2010 13th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2010. http://dx.doi.org/10.1115/icem2010-40284.
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This research addresses the potential of microbial implications in bentonite for use as a buffer and backfill material in final disposal site for low-level radioactive waste (LLRW) in Taiwan, where has a special island-type climate. Microbe activities naturally present in this site were analyzed, and buffer materials (BM) consisted of 100%, 70% or 50% bentonite were prepared for laboratory studies. A total of 39 microbial strains were isolated, and the predominant strains included four bacterial, one yeast and four fungal strains. Growth inhibition was not detected in any tested strain cultured in a radiation field with a dose rate of 0.2 Gy/h. Most of the isolated strains grew under a dose rate of 1.4 Gy/h. The D10 values of the tested strains ranged from 0.16 to 2.05 kGy. The mycelia of tested fungal strains could spread over 5 cm during six months of inoculation in BM. The spreading activity of the tested bacteria was less than that of the fungi. Moreover, biofilms were observed on the surfaces of the BM. Since a large and diverse population of microbes is present in Taiwan, microbes may contribute to the mobilization of radionuclides in the disposal site.
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Semenova, Anastasia Arturovna, Yulia Konstantinovna Yushina, Maria Alexandrovna Grudistova, Elena Viktorovna Zaiko, and Olga Evgenievna Ivanova. "STUDY OF MICROBIAL COMMUNITIES IN MEAT PROCESSING ENTERPRISES." In NEW TECHNOLOGIES IN MEDICINE, BIOLOGY, PHARMACOLOGY AND ECOLOGY. Institute of information technology, 2021. http://dx.doi.org/10.47501/978-5-6044060-1-4.30.
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The article discusses the results of a study of the microbial diversity of objects in the production environment of two meat processing enterprises, including antibiotic resistance, isolated strains of pathogenic microorganisms and their ability to biofilm formation.
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Kalzhekov, N., S. L. Bachtel, H. Posamentier, T. E. Playton, S. Jenkins, E. Iskakov, Z. Katrenov, P. Mitch Harris, and N. Tlepbergenov. "Seismic Geomorphology of Microbial-dominated Margin and Slope Environments around an Isolated Platform, Tengiz Field." In KazGeo 2012. Netherlands: EAGE Publications BV, 2012. http://dx.doi.org/10.3997/2214-4609.20142917.
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Kuzikova, Irina, Irina Kuzikova, Vera Safronova, Vera Safronova, Nadezda Medvedeva, and Nadezda Medvedeva. "IMPACT OF NONYLPHENOL ON THE PHYSIOLOGICAL ACTIVITY OF FUNGI FROM THE COASTAL AREA OF THE GULF OF FINLAND." In Managing risks to coastal regions and communities in a changing world. Academus Publishing, 2017. http://dx.doi.org/10.31519/conferencearticle_5b1b93c5890b52.86067390.
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Nonylphenol (NP) is the most abundant environmental estrogen listed as one of the priority hazardous substances in the Water Framework Directive (EC 2000) and the priority pollutant of Baltic Sea (HELCOM 2010). The present study aims to compare the effects of technical nonylphenol (tNP) on the cellulase, amylase and protease activity of the terrestrial fungal strains played a significant role in aquatic ecosystems due to their high adaptive capacity and a large range of functional activity. The study also attempts to understand the mechanisms behind the varying sensitivity of the terrestrial fungi to tNP. The fungal strains were isolated from the bottom sediments of the coastal area of the eastern part of the Gulf of Finland. The terrestrial fungi were identified based on their morphological characteristics and nucleotide sequence analysis of internal transcribed space region. One reason for significant differences in sensitivity to the toxicant studied among the fungi is the change in the fungal cell permeability, in particular in cell membrane permeability, induced by NP. Environmentally relevant concentrations of tNP cause significant changes in activity of hydrolytic enzymes in the terrestrial fungi Aspergillus tubingensis, Penicillium expansum, Penicillium glabrum, and Cadophora fastigiata involved in organic matter degradation in bottom sediments. There can be increasing or decreasing trend, depending on both the type of enzyme and the tNP concentration. The revealed changes may disrupt the destructive processes in bottom sediments, as well as succession and stability of microbial communities functioning in the aquatic environment. It was found that tNP contributes to the activation of proteolytic enzymes, considered as potential fungal virulence factors. This may lead to emergence fungal strains with enhanced virulence in aquatic microbiocenoses. The investigations of the physiological responses of terrestrial fungi under nonylphenol will be important for biochemical processes dynamics and their environmental consequences evaluation.
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Kuzikova, Irina, Irina Kuzikova, Vera Safronova, Vera Safronova, Nadezda Medvedeva, and Nadezda Medvedeva. "IMPACT OF NONYLPHENOL ON THE PHYSIOLOGICAL ACTIVITY OF FUNGI FROM THE COASTAL AREA OF THE GULF OF FINLAND." In Managing risks to coastal regions and communities in a changing world. Academus Publishing, 2017. http://dx.doi.org/10.21610/conferencearticle_58b431765a62a.
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Nonylphenol (NP) is the most abundant environmental estrogen listed as one of the priority hazardous substances in the Water Framework Directive (EC 2000) and the priority pollutant of Baltic Sea (HELCOM 2010). The present study aims to compare the effects of technical nonylphenol (tNP) on the cellulase, amylase and protease activity of the terrestrial fungal strains played a significant role in aquatic ecosystems due to their high adaptive capacity and a large range of functional activity. The study also attempts to understand the mechanisms behind the varying sensitivity of the terrestrial fungi to tNP. The fungal strains were isolated from the bottom sediments of the coastal area of the eastern part of the Gulf of Finland. The terrestrial fungi were identified based on their morphological characteristics and nucleotide sequence analysis of internal transcribed space region. One reason for significant differences in sensitivity to the toxicant studied among the fungi is the change in the fungal cell permeability, in particular in cell membrane permeability, induced by NP. Environmentally relevant concentrations of tNP cause significant changes in activity of hydrolytic enzymes in the terrestrial fungi Aspergillus tubingensis, Penicillium expansum, Penicillium glabrum, and Cadophora fastigiata involved in organic matter degradation in bottom sediments. There can be increasing or decreasing trend, depending on both the type of enzyme and the tNP concentration. The revealed changes may disrupt the destructive processes in bottom sediments, as well as succession and stability of microbial communities functioning in the aquatic environment. It was found that tNP contributes to the activation of proteolytic enzymes, considered as potential fungal virulence factors. This may lead to emergence fungal strains with enhanced virulence in aquatic microbiocenoses. The investigations of the physiological responses of terrestrial fungi under nonylphenol will be important for biochemical processes dynamics and their environmental consequences evaluation.
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Radu, Elena, F. Marinescu, I. Savin, M. Popa, G. Pircalabioru, Mihai Badic, and C. Chifiriuc. "MODULATION OF THE ANTIBIOTIC SUSCEPTIBILITY PROFILES OF SOME MICROBIAL STRAINS ISOLATED FROM WASTEWATER UNDER THE INFLUENCE OF THE ELECTROMAGNETIC FIELD." In International Symposium "The Environment and the Industry". National Research and Development Institute for Industrial Ecology, 2016. http://dx.doi.org/10.21698/simi.2016.0043.
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Sviridova, O. V., N. I. Vorobyov, Ya V. Pukhalsky, O. N. Kurchak, O. P. Onishchuk, V. I. Safronova, I. G. Kuznetsova, and V. N. Pishchik. "Ability of the soil cellulolytic bacteria to colonize endophytic niche of barley grains." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.20.
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To identify microorganisms that can penetrate into the endophytic niche of the grain of barley plants, many years of vegetative experiments were conducted on sod-podzolic soil without the use of mineral fertilizers. In the non-growing season, a biological product, consisting of cellulolytic association of bacteria with genotypic passport, decomposed barley straw. Presowing treatment of seeds was not carried out, therefore, during the growing season; local microorganisms decomposing plant residues could be present in the barley rhizosphere. After six years of rotation of barley plants, the microbiological composition of its seed niche was studied. As a result, it was found that in the seeds of barley bacteria are present in an amount of 240 ± 20 CFU/g of grain. Isolated pure cultures of microorganisms were identified as Cellulomonas gelida, Micrococcus luteus and Bacillus licheniformis by the sequence of ITS fragments of 16S rRNA. These types of bacteria were also present in the used biological product. Based on the research conducted, it can be assumed, that permanent cultivation of barley plants and sowing of seeds of the previous year can contribute to the formation of effective microbial and plant biosystems that are resistant to environmental stress.
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Miu, Lucreția, Ioana Lidia Ilea, Rodica Roxana Constantinescu, Madalina Ignat, Nicolae Catrina, Marcel Ionescu, Doina Creangă, and Emanuel Văcălie. "Case Study – Basement of the National Museum of Cotroceni." In The 9th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2022. http://dx.doi.org/10.24264/icams-2022.v.4.
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The work presents a series of aspects related to the degree of biodeterioration of some heritage objects made of inorganic substrates (brick), and the identification of fungi and bacteria with a damaging action on historical materials. Inorganic substrates represented by dust particles from walls and bricks were studied. Fired clay bricks can be severely affected by various macroscopic forms of degradation, as a consequence of chemical, physical-mechanical and biological degradation processes as a result of exposure to aggressive environmental agents. The samples studied (they were collected in compliance with conservation-restoration norms) are part of the “Small Cellar” of the Cotroceni Palace, one of the few spaces where the walls of the old foundation built by Șerban Cantacuzino between 1679-1681, are still preserved. By monitoring the microclimate parameters of the “small cellar” space, it was concluded that there is a greater variation of the relative humidity from 40% to 63%, but also of the temperature from 16℃ to 22℃, fluctuations due to the change of seasons. The samples taken, developed on the culture medium and isolated were analyzed from the point of view of microbiota, and the identified fungi mainly belong to the genera Mucor sp., Alternaria sp., Rhizopus sp., Fusarium sp. and Penicillium sp. Three different treatments, Clic, Q and Top Crete, were tested and applied on the wall by brushing. After 7 days, samples were collected and tested regarding microbial loading and the results are a proof of the treatment efficiency. The samples were further monitored from a microbiological point of view, but also from the point of view of the appearance given to the bricks after treatment (color change, brick texture, organoleptic properties, etc.).
Reports on the topic "Environmental microbial isolates"
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Reyis, N. W. Microbial dechlorination of PCBs in vitro studies using an anaerobic environmental isolate. Final report. Office of Scientific and Technical Information (OSTI), November 1990. http://dx.doi.org/10.2172/604381.
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Avni, Adi, and Gitta L. Coaker. Proteomic investigation of a tomato receptor like protein recognizing fungal pathogens. United States Department of Agriculture, January 2015. http://dx.doi.org/10.32747/2015.7600030.bard.
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Maximizing food production with minimal negative effects on the environment remains a long-term challenge for sustainable food production. Microbial pathogens cause devastating diseases, minimizing crop losses by controlling plant diseases can contribute significantly to this goal. All plants possess an innate immune system that is activated after recognition of microbial-derived molecules. The fungal protein Eix induces defense responses in tomato and tobacco. Plants recognize Eix through a leucine-rich-repeat receptor- like-protein (LRR-RLP) termed LeEix. Despite the knowledge obtained from studies on tomato, relatively little is known about signaling initiated by RLP-type immune receptors. The focus of this grant proposal is to generate a foundational understanding of how the tomato xylanase receptor LeEix2 signals to confer defense responses. LeEix2 recognition results in pattern triggered immunity (PTI). The grant has two main aims: (1) Isolate the LeEix2 protein complex in an active and resting state; (2) Examine the biological function of the identified proteins in relation to LeEix2 signaling upon perception of the xylanase elicitor Eix. We used two separate approaches to isolate receptor interacting proteins. Transgenic tomato plants expressing LeEix2 fused to the GFP tag were used to identify complex components at a resting and activated state. LeEix2 complexes were purified by mass spectrometry and associated proteins identified by mass spectrometry. We identified novel proteins that interact with LeEix receptor by proteomics analysis. We identified two dynamin related proteins (DRPs), a coiled coil – nucleotide binding site leucine rich repeat (SlNRC4a) protein. In the second approach we used the split ubiquitin yeast two hybrid (Y2H) screen system to identified receptor-like protein kinase At5g24010-like (SlRLK-like) (Solyc01g094920.2.1) as an interactor of LeEIX2. We examined the role of SlNRC4a in plant immunity. Co-immunoprecipitation demonstrates that SlNRC4a is able to associate with different PRRs. Physiological assays with specific elicitors revealed that SlNRC4a generally alters PRR-mediated responses. SlNRC4a overexpression enhances defense responses while silencing SlNRC4 reduces plant immunity. We propose that SlNRC4a acts as a non-canonical positive regulator of immunity mediated by diverse PRRs. Thus, SlNRC4a could link both intracellular and extracellular immune perception. SlDRP2A localizes at the plasma membrane. Overexpression of SlDRP2A increases the sub-population of LeEIX2 inVHAa1 endosomes, and enhances LeEIX2- and FLS2-mediated defense. The effect of SlDRP2A on induction of plant immunity highlights the importance of endomembrane components and endocytosis in signal propagation during plant immune . The interaction of LeEIX2 with SlRLK-like was verified using co- immunoprecipitation and a bimolecular fluorescence complementation assay. The defence responses induced by EIX were markedly reduced when SlRLK-like was over-expressed, and mutation of slrlk-likeusing CRISPR/Cas9 increased EIX- induced ethylene production and SlACSgene expression in tomato. Co-expression of SlRLK-like with different RLPs and RLKs led to their degradation, apparently through an endoplasmic reticulum-associated degradation process. We provided new knowledge and expertise relevant to expression of specific be exploited to enhance immunity in crops enabling the development of novel environmentally friendly disease control strategies.
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Mizrahi, Itzhak, and Bryan A. White. Uncovering rumen microbiome components shaping feed efficiency in dairy cows. United States Department of Agriculture, January 2015. http://dx.doi.org/10.32747/2015.7600020.bard.
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Ruminants provide human society with high quality food from non-human-edible resources, but their emissions negatively impact the environment via greenhouse gas production. The rumen and its resident microorganisms dictate both processes. The overall goal of this project was to determine whether a causal relationship exists between the rumen microbiome and the host animal's physiology, and if so, to isolate and examine the specific determinants that enable this causality. To this end, we divided the project into three specific parts: (1) determining the feed efficiency of 200 milking cows, (2) determining whether the feed- efficiency phenotype can be transferred by transplantation and (3) isolating and examining microbial consortia that can affect the feed-efficiency phenotype by their transplantation into germ-free ruminants. We finally included 1000 dairy cow metadata in our study that revealed a global core microbiome present in the rumen whose composition and abundance predicted many of the cows’ production phenotypes, including methane emission. Certain members of the core microbiome are heritable and have strong associations to cardinal rumen metabolites and fermentation products that govern the efficiency of milk production. These heritable core microbes therefore present primary targets for rumen manipulation towards sustainable and environmentally friendly agriculture. We then went beyond examining the metagenomic content, and asked whether microbes behave differently with relation to the host efficiency state. We sampled twelve animals with two extreme efficiency phenotypes, high efficiency and low efficiency where the first represents animals that maximize energy utilization from their feed whilst the later represents animals with very low utilization of the energy from their feed. Our analysis revealed differences in two host efficiency states in terms of the microbial expression profiles both with regards to protein identities and quantities. Another aim of the proposal was the cultivation of undescribed rumen microorganisms is one of the most important tasks in rumen microbiology. Our findings from phylogenetic analysis of cultured OTUs on the lower branches of the phylogenetic tree suggest that multifactorial traits govern cultivability. Interestingly, most of the cultured OTUs belonged to the rare rumen biosphere. These cultured OTUs could not be detected in the rumen microbiome, even when we surveyed it across 38 rumen microbiome samples. These findings add another unique dimension to the complexity of the rumen microbiome and suggest that a large number of different organisms can be cultured in a single cultivation effort. In the context of the grant, the establishment of ruminant germ-free facility was possible and preliminary experiments were successful, which open up the way for direct applications of the new concepts discovered here, prior to the larger scale implementation at the agricultural level.