Academic literature on the topic 'Enterococcus faecalis; biofilm; pH'
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Journal articles on the topic "Enterococcus faecalis; biofilm; pH"
Spiegelman, Lindsey, Adrian Bahn-Suh, Elizabeth T. Montaño, Ling Zhang, Greg L. Hura, Kathryn A. Patras, Amit Kumar, et al. "Strengthening of enterococcal biofilms by Esp." PLOS Pathogens 18, no. 9 (September 14, 2022): e1010829. http://dx.doi.org/10.1371/journal.ppat.1010829.
Full textHakiki, Dalhar, Latief Mooduto, Ketut Suardita, and Dian Agustin Wahjuningrum. "Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm." Conservative Dentistry Journal 7, no. 1 (September 27, 2019): 18. http://dx.doi.org/10.20473/cdj.v7i1.2017.18-22.
Full textRidhalaksani, Ridzki, Kamizar Nazar, Nila Kesuma Djauharie, Ratna Meidyawati, and Dewa Ayu Npa. "THE ANTIBACTERIAL POTENTIAL OF N-ACETYLCYSTEINE AS AN ENDODONTIC IRRIGANT ON THE CLINICAL ISOLATES OF THE ENTEROCOCCUS FAECALIS BIOFILM." International Journal of Applied Pharmaceutics 9 (January 1, 2018): 17. http://dx.doi.org/10.22159/ijap.2017.v9s2.05.
Full textZhan, Xiangjun, Yingzhu Tan, Yingmei Lv, Jianing Fang, Yuanjian Zhou, Xing Gao, Huimin Zhu, and Chao Shi. "The Antimicrobial and Antibiofilm Activity of Oregano Essential Oil against Enterococcus faecalis and Its Application in Chicken Breast." Foods 11, no. 15 (August 1, 2022): 2296. http://dx.doi.org/10.3390/foods11152296.
Full textAskora, Ahmed, Mohamed El-Telbany, Gamal El-Didamony, Eman Ariny, and Momen Askoura. "Characterization of φEf-vB1 prophage infecting oral Enterococcus faecalis and enhancing bacterial biofilm formation." Journal of Medical Microbiology 69, no. 9 (September 1, 2020): 1151–68. http://dx.doi.org/10.1099/jmm.0.001246.
Full textZancan, Rafaela Fernandes, Bruno Cavalini Cavenago, Denise Ferracioli Oda, Clovis Monteiro Bramante, Flaviana Bombarda de Andrade, and Marco Antonio Hungaro Duarte. "Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics." Brazilian Dental Journal 30, no. 6 (November 2019): 536–41. http://dx.doi.org/10.1590/0103-6440201902613.
Full textZainal, Fatin Faqihah, Nur Syafiqah Syamimi Suhaimi Suzey, Norzawani Jaffar, and Chew Ching Hoong. "The Influence of Lactobacillus Species on Nosocomial Pathogens’ Biofilm." Asian Journal of Medicine and Biomedicine 6, S1 (November 10, 2022): 178–80. http://dx.doi.org/10.37231/ajmb.2022.6.s1.578.
Full textWigler, Ronald, Shlomo Matalon, Tomer Goldberger, Anat Or Lerner, and Anda Kfir. "Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on Enterococcus faecalis Biofilm." Applied Sciences 10, no. 17 (September 2, 2020): 6096. http://dx.doi.org/10.3390/app10176096.
Full textvan Merode, Annet E. J., Henny C. van der Mei, Henk J. Busscher, Karola Waar, and Bastiaan P. Krom. "Enterococcus faecalis strains show culture heterogeneity in cell surface charge." Microbiology 152, no. 3 (March 1, 2006): 807–14. http://dx.doi.org/10.1099/mic.0.28460-0.
Full textAkaluka, Cynthia K., Justinah C. Orji, Wesley Braide, Emmanuel Egbadon, and Samuel A. Adeleye. "Abattoir Wastewater Treatment and Energy Recovery Using a Ferricyanide-Catholyte Microbial Fuel Cell." International Letters of Natural Sciences 55 (June 2016): 68–76. http://dx.doi.org/10.18052/www.scipress.com/ilns.55.68.
Full textDissertations / Theses on the topic "Enterococcus faecalis; biofilm; pH"
Tse, Chee-choong Micheal, and 謝志聰. "Effect of ultrasonic agitation on enterococcus faecalis biofilm." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45165993.
Full textChittezham, Thomas Vinai. "The molecular control and biological implications of autolysis in enterococcus faecalis biofilm development." Diss., Manhattan, Kan. : Kansas State University, 2009. http://hdl.handle.net/2097/1519.
Full textSchurig, Tilman David [Verfasser], and Karl-Thomas [Akademischer Betreuer] Wrbas. "Der antibakterielle Effekt verschiedener Wurzelkanalfülltechniken auf Enterococcus faecalis – Biofilm in humanen Wurzelkanälen in vitro." Freiburg : Universität, 2016. http://d-nb.info/112274336X/34.
Full textYang, Nan. "Role of Escherichia coli curli in relation with intestinal components - mucin, Klebsiella pneumoniae and Enterococcus faecalis." Phd thesis, INSA de Lyon, 2011. http://tel.archives-ouvertes.fr/tel-00657247.
Full textCastro, Pedro Coimbra de Almeida Osório de. "Biofilmes em endodontia." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4386.
Full textIntrodução: Um biofilme é uma comunidade estruturada de células bacterianas envolvidas numa matriz constituída por substâncias poliméricas e aderido a uma superfície sólida. Esta comunidade permite um modo protegido de crescimento que permite a sobrevivência dos constituintes celulares em ambientes hostis e fornece uma tolerância aumentada a agentes antimicrobianos. Objectivo: Tentar compreender a forma como os constituintes celulares se organizam e ter um melhor conhecimento das formas de resistência antimicrobiana características da organização em biofilmes, como também de rever os métodos actualmente usados para a desinfecção no tratamento endodôntico e abordando métodos alternativos ainda em estudo. Materiais e métodos: Para tal realizou-se uma pesquisa bibliográfica nos principais motores de busca: Pubmed, B-On, SciELO, Science Direct, como também no repositório da Universidade Fernando Pessoa e da Faculdade de Medicina Dentária do Porto utilizando as palavras-chave “Biofilms”, “Apical Periodontitis”, “Enterococcus faecalis” e “Biofilm Treatment “ que foram associadas de várias formas. Desta pesquisa efectuada, entre Junho de 2014 e Julho de 2014, foram escolhidos 117 artigos em Português e Inglês dos quais foram usados 89. Resultados: A forma como actualmente procedemos à desinfecção do sistema de canais radiculares, passando pela instrumentação mecânica e irrigação química não é totalmente satisfatória no que toca a uma total erradicação dos microorganismos devido a várias limitações como a complexidade anatómica dos canais e a ecologia presente no interior dos mesmos. Conclusões: De futuro, terão que ser desenvolvidas outras estratégias antimicrobianas para suplementar as existentes. Embora estas pareçam promissoras in vitro elas carecem de estudos in vivo, os quais serão necessários no futuro para ultrapassar as várias limitações presentes no sistema de canais radiculares. Introduction: A biofilm is a structured bacterial cell community enveloped in a matrix composed of polymeric substances and attached to a solid surface. This community allows for a protected way of growth that permits the survival of the cellular components in hostile environments and provides a higher tolerance to antimicrobial agents. Objective: Trying to understand the way cellular components are organized and have a better knowledge of the antimicrobial resistances that are characteristic of the way biofilms are structured, as well as to review the currently used methods for disinfection in an endodontic treatment and to address alternative methods still in study. Materials and Methods: To this end a bibliographic research was performed on the main search engines: Pubmed, B-On, SciELO, Science Direct, and also on Universidade Fernando Pessoa and Faculdade de Medicina Dentária do Porto’s repository using “Biofilms”, “Apical Periodontitis”, “Enterococcus faecalis” and “Biofilm Treatment“ as key-words that were associated in many forms. From this research performed between June 2014 and July 2014, were selected 117 articles in Portuguese and English and from those, 89 were used. Results: The way that we currently proceed regarding the disinfection of the root canal system using mechanical instrumentation and chemical irrigation is not fully satisfactory, when it comes to the total eradication of the microorganisms present due to several limitations like the complexity of the root canal anatomy and the ecology present inside the root canal. Conclusions: In the future, other antimicrobial strategies will have to be developed to supplement the currently used ones. Although these look promising in vitro they lack in vivo studies, that will be necessary in the future to overcome the several limitations present in the root canal system.
Iyer, Vijayalakshmi Subramanian. "Role of the transcription regulator RpoN (sigma 54) in Enterococcus faecalis biofilm development, metabolism and virulence." Diss., Kansas State University, 2012. http://hdl.handle.net/2097/17150.
Full textDepartment of Biology
Lynn Hancock
Enterococci are the third leading cause of nosocomial infections including urinary tract infections (UTI), surgical site infections (SSI) and blood stream infections. Enterococci are also found in the gastrointestinal tracts of humans, and other mammals. We elucidated the influence of the transcriptional regulator RpoN on enterococcal biofilm formation, virulence potential and cell wall architecture and proposed a potential involvement for carbohydrate metabolism in these processes. Biofilms are held together by matrix (BM) components such as extracellular DNA (eDNA) released by cell death from a sub-population of cells. The rpoN mutant (ΔrpoN) was resistant to autolysis as well as fratricide-mediated cell death and eDNA was not detected in planktonic as well as biofilm cultures. Unlike the parental strain V583, the ΔrpoN mutant formed proteinase K sensitive biofilms, suggesting that protein as well as eDNA serves as an important matrix component. The rabbit model of endocarditis was used to assess the effect of rpoN deletion on enterococcal virulence. Rabbits infected with ΔrpoN had reduced bacterial burden in heart, blood, liver, kidney and vegetation in comparison to the parental strain. The growth defect of ΔrpoN in physiologically relevant glucose levels (5 mM) partially explains the reduced bacterial burdens observed in the virulence study. Microarray analysis of ΔrpoN showed that 10% of the genome is differentially regulated by RpoN. Deletion of rpoN also protects Enterococcus faecalis from lysis in the absence of known modulators of cellular lytic events such as O-acetylation and D-alanylation. Of the four identified enhancer binding proteins in E. faecalis, MptR regulates the RpoN-dependent mannose/glucose uptake system (MptABCD) and the ΔmptR mutant phenocopied the ΔrpoN mutant in the eDNA release and growth assays. Because MptC and MptD have been shown to be the cellular receptors for class IIa and IIc bacteriocins, we are presently testing the hypothesis that these receptors may serve as a global receptor for bacteriocins. In conclusion, our data demonstrates that alterations in the metabolic state of the bacterium, as observed in the ΔrpoN mutant could be responsible for the switch in biofilm matrix composition, and this switch in turn likely influences the virulence potential of the bacterium.
Alves, Denise Ramos Silveira. "Efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis e estrutura dentinária." Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/6229.
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Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
Objective: Evaluate the effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in infected root canals and on dentin structure. Methods: Twenty-one root canals of a sample of 24 extracted single-rooted human teeth were infected by E. faecalis for 60 days to form biofilm. The antimicrobial strategies tested were (n=3 in each group): root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group I) or 0.01% malachite green (group II); root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group III); PDT with 0.01% methylene blue without root canal preparation (group IV); root canal preparation using NiTi rotary instruments, 22.5% NaOCl and 17% EDTA irrigation, and no PDT (group V); 2.5% NaOCl irrigation with no root canal preparation, and 17% EDTA irrigation (group VI); positive control (group VII). Three roots were not infected and were used as negative controls (group VIII). Samples for microbiological tests were collected using three sterile paper points, later stored in BHI and incubated at 37o C for 48 hours at three time points: before (S1) and after (S2) root canal preparation, and after PDT application (S3). Bacterial growth was analyzed according to turbidity of culture medium, presence of bacteria, and spectrophotometric optical density (nm). Specimens were sectioned and prepared for SEM analysis of dentin structure. Results: Bacteria were found at S1, S2 and S3 in all experimental groups. Optical density of culture media at S2 and S3 in groups I, II and III were lower than at S1, but not statistically different. Optical density of culture media at S2 was 28.70% and 24.67% lower than at S1 in groups I and II; after PDT, optical density was 90.00% (group I) and 37.30% (group II) lower. In group III, it was 97.70% lower at S2 and an additional 92.00% lower after PDT. In group IV, optical density increase 3.2%. Dentin analysis after PDT revealed areas of melting and recrystallization, peritubular dentin projections, intertubular dentin erosion and fusion of dentinal tubule openings, which made dentin surface irregular. Some dentinal tubules were obliterated, and there were changes in the shape of their openings. Conclusion: PDT applied after root canal preparation using manual or rotary files was not effective in eliminating E. faecalis completely. PDT changed dentin structure and resulted in dentin melting and recrystallization, as well as in dentinal tubule erosion and obliteration.
Objetivo: Avaliar o efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis em canais radiculares infectados e sobre a estrutura dentinária. Metodologia: O estudo foi desenvolvido em vinte e quatro dentes humanos unirradiculares extraídos, dos quais vinte e um canais radiculares foram infectados com E. faecalis por 60 dias para formação de biofilme. As estratégias antimicrobianas testadas foram (n=3): preparo do canal radicular com instrumentos rotatórios de NiTi/ NaOCl 2,5%/ irrigação final EDTA 17%, e TFD com azul de metileno 0,01% (Grupo I) ou verde malaquita 0,01% (Grupo II); preparo do canal radicular com instrumentos manuais de aço inox/ NaOCl 2,5%/ irrigação final EDTA 17% e TFD com azul de metileno 0,01% (Grupo III); TFD com azul de metileno 0,01% sem preparo prévio do canal radicular (Grupo IV); preparo do canal radicular com instrumentos rotatórios de NiTi/ NaOCl 2,5%/ irrigação final EDTA 17% sem emprego da TFD (Grupo V); irrigação com hipoclorito de sódio 2,5% sem preparo do canal radicular/ irrigação final EDTA 17% (Grupo VI); controle positivo (Grupo VII). Três espécimes não foram contaminados, sendo utilizados como controle negativo (Grupo VIII). Coletas microbiológicas foram realizadas, antes (CM1) e após (CM2) o preparo do canal radicular, e depois da aplicação da TFD (CM3), utilizando três pontas de papel absorventes esterilizadas, posteriormente armazenadas em BHI e a seguir , incubadas a 37o C por 48 horas. O crescimento bacteriano foi analisado pela turbidez do meio de cultura, sendo determinada a presença ou ausência de bactérias, e pela densidade óptica do meio de cultura, interpretada por espectrofotometria (nm). A seguir, os espécimes foram seccionados e preparados para análise da estrutura dentinária por meio de imagens de MEV. Resultados: A presença de bactérias foi verificada na CM1 , CM2 e CM3 de todos os grupos experimentais. As medidas da densidade óptica dos meios de cultura das CM2 e CM3 nos grupos experimentais I, II e III apresentaram redução quando comparada a CM1, porém não significativa estatisticamente. Nos Grupos I e II a densidade óptica do meio de cultura foi reduzida em 28.70% e 24,67% em CM2, respectivamente. Após a TFD, a redução da densidade óptica foi 90,00% (Grupo I) e 37,70% (Grupo II). No Grupo III, a redução da densidade óptica do meio de cultura foi de 97,70% na CM2, com redução adicional de 92,00% após TFD. No Grupo IV foi verificado aumento da densidade óptica do meio de cultura em 3,2%. A análise da dentina evidenciou, nos grupos submetidos à TFD, áreas de derretimento e recristalização, projeção da dentina peritubular, e regiões com erosão da dentina intertubular e união das entradas dos túbulos dentinários, tornando a superfície dentinária irregular. Obliteração de túbulos dentinários com alteração do contorno de suas entradas também foi verificada. Conclusão: A TFD, após preparo do canal radicular com sistema rotatório ou manual, não foi efetiva na eliminação completa de E. faecalis, e alterou a estrutura dentinária, determinando derretimento e recristalização de dentina, erosão e obliteração de túbulos dentinários.
Rosa, Ricardo Abreu da. "Efetividade da terapia fotodinâmica associada à soluções irrigadoras frente a dois modelos de biofilme." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/144218.
Full textThe aim of this study was to evaluate the antibacterial effect and the biofilm dissolution promoted by photodynamic therapy (PDT) associated with 2.5% NaOCl and 2% CHX over monospecies and multispecies biofilms. In monospecies biofilm model, forty-six mandibular premolars were contaminated with Enterococcus faecalis strains (ATCC 29212) broth culture for 21 days. Specimens were divided into three groups according to the irrigant used: saline, 2% of CHX and 2.5% of NaOCl. After irrigation with 5 mL of each irrigant, PDT was performed. Samples were collected at baseline (S1), after irrigation (S2) and after PDT (S3). In multispecies biofilm model, sixty bovine dentin blocs were infected intraorally, and divided into six groups: saline, saline/PDT, CHX, CHX/PDT, NaOCl and NaOCl/PDT. Confocal laser scanning microscopy was used to assess the percentage and the biovolume of live cells and the total biovolume. All groups reduced UFC’ counts after irrigation procedures (S1-S2); however CHX and NaOCl promoted the lowest UFCs counts (P < 0.05). PDT significantly reduced the bacterial counts in saline group (S2-S3; P < 0.05). In multispecies biofilm model, the lowest amount of live cells was observed in CHX, CHX/PDT, NaOCl and NaOCl/PDT groups, with no differences among them (P > 0.05). PDT did not reduce the total biovolume (P > 0.05); however it appears to decrease the biovolume and the amount of live cells after irrigation with 2% CHX and 2.5% NaOCl. PDT associated with saline reduced the bacterial load in canal infected with E. faecalis. PDT seems to reduce the amount and the biovolume of live cells, but did not reduce the total biovolume of cells in multispecies biofilm induced in situ. Finally, the irrigant was decisive to dissolve multispecies biofilm.
Chettaoui, Rayane. "Activité anti-biofilm du cranberry et de l’un de ses métabolites envers Enterococcus faecalis dans un contexte d’infection urinaire." Thesis, Cergy-Pontoise, 2017. http://www.theses.fr/2017CERG0909.
Full textEscherichia coli and Enterococcus faecalis are two main pathogens involved in urinary tract infections (ITU) in town medicine and in the hospital. These bacterial species are responsible for acute UTIs with recurrence phenomena and in chronic ITUs. The consumption of antibiotics is directly correlated with the resistance of uropathogenic bacteria, which shows the importance of controlling the use of antibiotics and of developing alternative preventive and curative treatments for urinary infections.Cranberry consumption of their extracts is traditionally associated with the maintenance of healthy urinary tract. In addition, some clinical studies seem to show a preventive effect of ITUs associated with cranberry consumption. In vitro and ex vivo, the consumption of these extracts by humans reduces the adhesion of certain E. coli strains to urinary epithelial cells and biofilm formation of different species. The working hypothesis is that the consumption of cranberry extracts leads to the formation of urinary metabolites that decrease the adhesion of uropathogenic bacteria to the urinary epithelium. This mechanism would be the basis for the prevention of ITU by consumption of cranberry extracts. However, bioactive metabolites remain largely unknown
Ardalan, Cyrous. "A Comparative Study of Intraradicular Enterococcus Faecalis Biofilm Removal with Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4741.
Full textBooks on the topic "Enterococcus faecalis; biofilm; pH"
Bao, Yinyin. Role of mprF1 and mprF2 in the pathogenicity of Enterococcus faecalis. Freiburg: Universität, 2012.
Find full textBook chapters on the topic "Enterococcus faecalis; biofilm; pH"
Kumar Oli, Ajay, Palaksha K. Javaregowda, Apoorva Jain, and Chandrakanth R. Kelmani. "Mechanism Involved in Biofilm Formation of Enterococcus faecalis." In Bacterial Biofilms [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.103949.
Full textBhonchal Bhardwaj, Sonia, and Seema Kumari. "Oral Bacteriophages." In Bacteriophages in Therapeutics. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.100269.
Full textRaja, Kavita. "Enterococcal Infections: Recent Nomenclature and emerging trends." In Streptococcal Infections [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.104792.
Full textConference papers on the topic "Enterococcus faecalis; biofilm; pH"
Ke Sun, Jue Zhang, Jing Fang, Jing Wang, Jie Pan, and Weidong Zhu. "Cold plasma therapy for enterococcus faecalis biofilm infected tooth root canal in vitro." In 2012 IEEE 39th International Conference on Plasma Sciences (ICOPS). IEEE, 2012. http://dx.doi.org/10.1109/plasma.2012.6383868.
Full textPereira, Ulisses A., Luiz C. A. Barbosa, Célia R. A. Maltha, Antônio Jacinto Demuner, and Andréa de L. Pimenta. "Synthesis of lactones and lactams analogues to rubrolides as inhibitors of Enterococcus faecalis biofilm formation." In 15th Brazilian Meeting on Organic Synthesis. São Paulo: Editora Edgard Blücher, 2013. http://dx.doi.org/10.5151/chempro-15bmos-bmos2013_2013814154045.
Full textLi, Yinglong, Guomin Wang, Jue Zhang, Jing Fang, Jie Pan, and Weidong Zhu. "Evaluation of cold plasma treatment and safety in disinfecting 21-day root canal enterococcus faecalis biofilm in vitro." In 2014 IEEE 41st International Conference on Plasma Sciences (ICOPS) held with 2014 IEEE International Conference on High-Power Particle Beams (BEAMS). IEEE, 2014. http://dx.doi.org/10.1109/plasma.2014.7012693.
Full textHidayati, Rizka, Ari Asnani, Muhamad Salman Fareza, and Dwi Utami Anjarwati. "In Vitro Study of Reduction of Oral Enterococcus faecalis Biofilm on Application of Combination of Chrysomya megacephala Maggot Extract and Sodium Hypochlorite." In 1’s t Jenderal Soedirman International Medical Conference (JIMC) in conjunction with the Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia (KIBI ). SCITEPRESS - Science and Technology Publications, 2020. http://dx.doi.org/10.5220/0010488400980103.
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