Relevant bibliographies by topics / Enterococcus faecalis

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Academic literature on the topic 'Enterococcus faecalis'

Author: Grafiati
Published: 4 June 2021
Last updated: 7 July 2024

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Journal articles on the topic "Enterococcus faecalis"

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Maheux, Andrée F., Sébastien Bouchard, Ève Bérubé, and Michel G. Bergeron. "Rapid molecular identification of fecal origin-colonies growing on Enterococcus spp.-specific culture methods." Journal of Water and Health 15, no. 2 (December 17, 2016): 239–50. http://dx.doi.org/10.2166/wh.2016.199.

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The mEI, Chromocult® enterococci, and m-Enterococcus culture-based methods used to assess water quality by the detection of Enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of Enterococcus spp. and (2) environmental colonies identified by 16S rRNA sequencing. Then, two specific-rtPCR assays targeting Enterococcus spp. and Enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. The mEI, Chromocult® enterococci, and m-Enterococcus methods detected β-glucosidase activity for 28 (68.3%), 32 (78.0%), and 12 (29.3%) of the 41 reference enterococcal strains tested, respectively. Analysis with environmental colonies showed that mEI and Chromocult® enterococci media had false positive rates of 4.3% and 5.0%, respectively. Finally, the two rtPCR assays showed a specificity of 100%. Only two (2/19) colonies of E. faecium isolated from mEI agar were not detected by the Enterococcus faecium rtPCR assay, for a sensitivity of 89.5%. Our results showed that Chromocult® enterococci medium recovered more E. faecalis/faecium cells than the two other methods. Thus, the use of Chromocult® enterococci combined with the Enterococcus faecalis/faecium rtPCR assay showed the best combination to decrease the high false-positive rate obtained when the entire Enterococcus genus is targeted.
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Kumurya, A. S., and B. Ega. "An Overview on Vancomycin Resistant Enterococcus faecalis." UMYU Journal of Microbiology Research (UJMR) 6, no. 1 (June 30, 2021): 160–67. http://dx.doi.org/10.47430/ujmr.2161.033.

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There are over 15 species of the Enterococcus genus, 80-90% of clinical isolates as E. faecalis. The aim of this work is to review the current information on Vancomycin resistant Enterococcus fecalis. The study reviewed using electronic documents and hard copies from public libraries of relevant literatures relating to biology, epidemiology, drug resistance mechanism, treatment, and control of Enterococcus faecalis. The review revealed that Enterocuccus faecalis formerly known as Streptococcus faecalis is a Gram-positive commensal bacterium that inhabits the gastrointestinal tracts of healthy humans and other mammals. However, it can cause lifethreatening infections in humans, especially in the nosocomial environment, where there are naturally high levels of antibiotic resistance. Thus, Enterococci have proven to present a therapeutic challenge because of their resistance to many antimicrobial drugs, including cell-wall active agents; aminoglycosides, penicillin, ampicillin, and vancomycin.” The Enterococci have the capacity to acquire a wide variety of antimicrobial resistance factors through plasmid transfer by conjugation, which present serious problems in the management of patients with Enterococcal infections. In general, Enterococcal isolates with lowered susceptibility to vancomycin are categorized as vanA, vanB, and vanC, vanA and vanB pose the greatest threat because they are the most resistant genes.E. faecalis are also resistant to teicoplanin. Enterococcal strains that are vancomycin-dependent have been found, but are rare and less common than vancomycin-resistant strains (referred to as “vancomycin-resistant Enterococci” or “VRE”). The review, identified that although VRE infection possess the tendency to become endemic especially in very ill debilitated patients who have been exposed to broad spectrum antibiotics; and the immune-compromised, yet Vancomycin continues to be the drug of choice for serious life threatening infections as sepsis, pneumonia, and endocarditis. Keywords: Vancomycin-resistant Enterococci(VRE), Enterococcus faecalis, Resistance gene
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Mustafa, Eman, and Amera Al-Rawi. "Molecular investigation of enterococcal surface protein (esp) gene of Enterococcus faecalis isolated from endodontic patients." Medicinski casopis 57, no. 4 (2023): 141–46. http://dx.doi.org/10.5937/mckg57-46998.

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Objective. Enterococci are generally considered transient components of oral bacteria that may be a reason for several oral and systemic infections, particularly those related to dental root canal infections. The current study aims to examine the occurrence of Enterococcus surface protein, esp in Enterococcus faecalis, which is isolated from infected root canals. Methods. Forty samples were collected from endodontic patients who attended the Conservative Treatment Department in the College of Dentistry/Mosul University/Dental Teaching Hospital. Materials and Methods: All samples were traditionally examined using HiCrom TM Enterococcus faecium Agar base medium and biochemical tests. 16srRNA sequencing was performed using the polymerase chain reaction technique to confirm their identity. Then, all Enterococcus faecalis isolates were examined for the existence of esp gene coding for enterococcal surface protein using PCR assay. Results. From 40 clinical samples obtained, 31 isolates were recognized as E. faecalis by traditional methods; unexpectedly, other non-enterococci genera were also grown on HiCromTM Enterococcus faecium Agar base medium. The PCR products for the sequence-specific primers obtained from the full-length of 16S rRNA gene sequence, which belongs to E. faecalis, and the PCR products for specific primer of esp genes created bands at the position of 138bp and 932 bp on the agarose gel, respectively. The gene correlating with the aggregation of this bacteria on the canal walls was detected in a high proportion (91%) of the isolates. Conclusions. PCR assay provides an accurate, rapid, and more sensitive detection of E. faecalis. A positive correlation between esp gene and enterococcal infections in root canals has been found.
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KIM, YEONG BIN, HYUN JOO SEO, KWANG WON SEO, HYE YOUNG JEON, DONG KYU KIM, SHIN WOO KIM, SUK-KYUNG LIM, and YOUNG JU LEE. "Characteristics of High-Level Ciprofloxacin-Resistant Enterococcus faecalis and Enterococcus faecium from Retail Chicken Meat in Korea." Journal of Food Protection 81, no. 8 (July 17, 2018): 1357–63. http://dx.doi.org/10.4315/0362-028x.jfp-18-046.

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ABSTRACTGenes encoding ciprofloxacin resistance in enterococci in animals may be transferred to bacteria in the animal gut and to zoonotic bacteria where they could pose a human health hazard. The objective of this study was to characterize antimicrobial resistance in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from retail chicken meat. A total of 345 enterococci (335 E. faecalis and 10 E. faecium) were isolated from 200 chicken meat samples. Of these, 85 E. faecalis isolates and 1 E. faecium isolate were confirmed as HLCR enterococci. All 86 HLCR enterococci displayed gyrA-parC point mutations consisting of S83I-S80I (94.2%, 81 isolates), S83F-S80I (2.3%, 2 isolates), S83Y-S80I (2.3%, 2 isolates), and S83Y-S80F (1.2%, 1 isolate). Sixty-one (72.9%) of the 86 HLCR enterococci showed multidrug resistance to three to six classes of antimicrobial agents. Multilocus sequence typing revealed that E. faecalis had 17 different sequence types (ST) and E. faecium had 1 different ST, with ST256 observed most often (44 isolates, 51.8%). Although these results cannot exclude the possibility that pathotypes of enterococci isolated from chicken might represent transmission to or from humans, the foodborne HLCR E. faecalis indicated that the food chain is a potential route of enterococcal infection in humans.
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KOBAYASHI, N., MD MAHBUB ALAM, Y. NISHIMOTO, S. URASAWA, N. UEHARA, and N. WATANABE. "Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium." Epidemiology and Infection 126, no. 2 (April 2001): 197–204. http://dx.doi.org/10.1017/s0950268801005271.

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Aminoglycoside modifying enzymes (AMEs) are major factors which confer aminoglycoside resistance on bacteria. Distribution of genes encoding seven AMEs was investigated by multiplex PCR for 279 recent clinical isolates of enterococci derived from a university hospital in Japan. The aac(6′)-aph(2″), which is related to high level gentamicin resistance, was detected at higher frequency in Enterococcus faecalis (42·5 %) than in Enterococcus faecium (4·3 %). Almost half of E. faecalis and E. faecium isolates possessed ant(6)-Ia and aph(3′)-IIIa. The profile of AME gene(s) detected most frequently in individual strains of E. faecalis was aac(6′)-aph(2″)+ant(6)-Ia+aph(3′)-IIIa, and isolates with this profile showed high level resistance to both gentamicin and streptomycin. In contrast, AME gene profiles of aac(6′)-Ii+ant(6)-Ia+aph(3′)-IIIa, followed by aac(6′)-Ii alone, were predominant in E. faecium. Only one AME gene profile of ant(6)-Ia+aph(3′)-IIIa was found in Enterococcus avium. The ant(4′)-Ia and ant(9)-Ia, which have been known to be distributed mostly among Staphylococcus aureus strains, were detected in a few enterococcal strains. An AME gene aph(2″)-Ic was not detected in any isolates of the three enterococcal species. These findings indicated a variety of distribution profiles of AME genes among enterococci in our study site.
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Dolka, Beata, Mariola Gołębiewska–Kosakowska, Krzysztof Krajewski, Piotr Kwieciński, Tomasz Nowak, Jarosław Szubstarski, Jarosław Wilczyński, and Piotr Szeleszczuk. "Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data." Medycyna Weterynaryjna 73, no. 4 (2017): 220–24. http://dx.doi.org/10.21521/mw.5680.

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Bacteria of the genus Enterococcus are mainly commensals building natural microflora in the digestive tract of birds and mammals. They belong to the potentially pathogenic microorganisms. Among poultry, infections caused by enterococci were reported in chickens, turkeys, ducks and ostriches. The aim of this study was to evaluate the occurrence of enterococci in poultry in Poland, including identification of enterococcus species composition and determination of the age of birds. The analysis was based on data obtained from 2014 – 2015 from Division of Avian Diseases at Warsaw University of Life Sciences-SGGW and four veterinary laboratories in Poland: Lab – Vet, Tarnowo Podgórne; SLW Biolab, Ostróda; Vetdiagnostica, Solec Kujawski; Vet – Lab Brudzew. Seven enterococcal species were isolated from broiler chickens (CB), commercial layers (CL), and broiler breeder flocks (BB), nine from all poultry types (chickens, turkey, ducks and geese). The most often isolated enterococci from CB were E. faecalis (57%) > E. cecorum (7%) > E. faecium (5.2%) > E. hirae (3.6%) > E. gallinarum (2.5%) > E. casseliflavus (0.7%) > E. durans (0.2%). Seven Enterococcus species were isolated from sources associated with poultry, most often E. faecalis > E. faecium > E. cecorum > E. hirae. The differences in the occurrence of particular enterococcal species were observed between CB, BB and CL. The mean age at the time of isolation of E. cecorum was approx.: 3.6 weeks in CB, 27.5 weeks in BB, 33.3 weeks in CL, 12.9 weeks in turkeys, 3.6 weeks in ducks, 39.5 weeks in geese. E. faecalis and E. faecium dominated in samples obtained from hatching eggs, dead-in-shell embryos and from samples related to poultry environment. In conclusion, this study indicates the high prevalence of bacteria of the Enterococcus genus in poultry. The present findings demonstrate the differences in Enterococcus species between poultry groups, including with regard to age. In total 10 enterococcal species (E. faecalis, E. cecorum, E. hirae, E. faecium, E. gallinarum, E. casseliflavus, E. durans, E. avium E. thailandicus, E. aquimarinus) were detected in poultry, poultry environmental samples, hatching eggs and dead-in-shell embryos. Enterococcus faecalis and E. cecorum were found in all above-mentioned sources.
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Tsiodras, Sotirios, Howard S. Gold, Eoin P. G. Coakley, Christine Wennersten, Robert C. Moellering, and George M. Eliopoulos. "Diversity of Domain V of 23S rRNA Gene Sequence in Different Enterococcus Species." Journal of Clinical Microbiology 38, no. 11 (2000): 3991–93. http://dx.doi.org/10.1128/jcm.38.11.3991-3993.2000.

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The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species ofEnterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, andEnterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed fromE. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.
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CHINGWARU, W., S. F. MPUCHANE, and B. A. GASHE. "Enterococcus faecalis and Enterococcus faecium Isolates from Milk, Beef, and Chicken and Their Antibiotic Resistance." Journal of Food Protection 66, no. 6 (June 1, 2003): 931–36. http://dx.doi.org/10.4315/0362-028x-66.6.931.

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The occurrence and antibiotic resistance of enterococci, especially Enterococcus faecalis and Enterococcus faecium, in milk, beef, and chicken in Gaborone, Botswana, were studied. Enterococci were isolated from these sources with the use of bile esculin agar and identified with API 20 Strep kits. Antibiotic resistance was determined by the disk diffusion method. The antibiotics tested were vancomycin, teicoplanin, ampicillin, tetracycline, and cephalothin. Among the 1,467 enterococci isolated from the samples, E. faecalis (46.1%) and E. faecium (29.0%) were found to be the predominant species. Other enterococcal species made up 25% of the isolates. More than 96 and 97% of the E. faecalis and E. faecium isolates, respectively, were found to be resistant to ampicillin. Almost 34, 27.3, and 22.4% of the E. faecalis isolates from milk, beef, and chicken, respectively, were also resistant to cephalothin. The percentages of E. faecium isolates that were found to be resistant to cephalothin were 32.8, 16.9, and 17.3% for milk, beef, and chicken, respectively. Resistance to vancomycin was widespread. It was found that 18.8, 7.8, and 13.1% of the E. faecalis isolates from milk, beef, and chicken samples, respectively, were resistant to vancomycin. In contrast, 32.8, 24.7, and 30.7% of the E. faecium isolates from milk, beef, and chicken samples, respectively, were resistant to vancomycin. Isolates that were resistant to multiple drugs were found in relatively large numbers.
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Cox, Christopher R., and Michael S. Gilmore. "Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis." Infection and Immunity 75, no. 4 (January 12, 2007): 1565–76. http://dx.doi.org/10.1128/iai.01496-06.

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ABSTRACT Enterococci are commensal organisms of the gastrointestinal (GI) tracts of a broad range of mammalian and insect hosts, but they are also leading causes of nosocomial infection. Little is known about the ecological role of enterococci in the GI tract consortia. To develop a tractable model for studying the roles of these organisms as commensals and pathogens, we characterized the Drosophila melanogaster microflora and examined the occurrence of enterococci in the gastrointestinal consortium of Drosophila. In a survey of laboratory-reared Drosophila and wild-captured flies, we found that Drosophila was naturally colonized by representatives of five bacterial phyla. Among these organisms were several species of enterococci, including Enterococcus faecalis, Enterococcus faecium, Enterococcus gallinaraum, and Enterococcus durans, as well as a previously detected but uncultured Enterococcus species. Drosophila could be cured of enterococcal carriage by antibiotic treatment and could be reassociated with laboratory strains. High-level colonization by a well-characterized strain expressing the enterococcal cytolysin was found to be detrimental to Drosophila compared to the effect of an isogenic, noncytolytic control. The anatomical distribution of enterococci in the Drosophila GI tract was determined by immunohistochemical staining of thin sections of naturally colonized and reassociated flies.
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Weisser, Maja, Selja Capaul, Marc Dangel, Luigia Elzi, Esther Kuenzli, Reno Frei, and Andreas Widmer. "Additive Effect of Enterococcus faecium on Enterococcal Bloodstream Infections: A 14-Year Study in a Swiss Tertiary Hospital." Infection Control & Hospital Epidemiology 34, no. 10 (October 2013): 1109–12. http://dx.doi.org/10.1086/673145.

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We investigated whether an increase in enterococcal bloodstream infections (BSIs) depends on the emergence of Enterococcus faecium in an area with low vancomycin-resistant enterococci prevalence. From 1999 to 2012, a linear increase in E. faecium BSI rates (0.009 per 1,000 patient-days per year; P<.001) was noted. Enterococcus faecalis BSI rates remained stable.
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More sources

Dissertations / Theses on the topic "Enterococcus faecalis"

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Roberts, Gretta. "Glycoprotein utilisation by enterococcus faecalis." Thesis, King's College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412446.

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Barros, Mariana Vilhena. "Infeções nosocomiais por enterococcus faecalis." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4512.

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Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas
As infeções nosocomiais são consideradas um problema mundial de saúde pública. A sua disseminação tem contribuído para aumento das taxas de mortalidade e morbilidade, a maioria das vezes devido às limitadas ou mesmo inexistentes opções terapêuticas. Enterococcus faecalis é uma bactéria Gram-positiva, anaeróbia facultativa, presente na flora comensal do trato gastrintestinal de humanos e animais. Apesar da sua suposta inocuidade, nas últimas décadas E.faecalis tem-se revelado um patogénio oportunista, representando a segunda e a terceira maior causa de infeções hospitalares a nível mundial. Esta bactéria é apontada como uma das principais causas de endocardites, bacteremias, infeções do trato urinário, intra-abdominais e de feridas contraídas em hospitais. As suas caraterísticas fisiológicas permitem-lhe sobreviver a altas temperaturas, a elevados valores de pH e concentrações salinas. Esta bactéria resiste também em ambientes hostis, em situações de subnutrição, de stress oxidativo e às técnicas tradicionais de limpeza. Apresenta inúmeros fatores de virulência nomeadamente proteínas de superfície, enzimas hidrolíticas e capacidade de formação de biofilmes, o que auxilia esta bactéria a invadir, colonizar e infetar tecidos hospedeiros. Enterococcus faecalis exibe uma resistência intrínseca a algumas classes de antibióticos como β-lactâmicos, lincosamidas, trimetropim-sulfametoxazol, fluoroquinolonas e baixas concentrações de aminoglícosídeos. Devido à sua capacidade mutagénica e adaptativa, este microrganismo desenvolve e adquire novas resistências, através de mutações cromossomais ou por transferência de genes. Como é o caso do gene vanA e vanB, que lhe conferem resistência à vancomicina (VRE). E.faecalis tem-se revelado uma constante ameaça de vida em todo o mundo, por isso é urgente controlar a disseminação desta bactéria. É importante desenvolver novos métodos terapêuticos, tendo em conta a ineficácia dos atuais e aplicar estratégias, a nível hospitalar, que diminuam ao máximo a transmissão do microrganismo. The nosocomial infections are nowadays a worldwide issue in terms of health. Its dissemination has been the cause of the rising of mortality and disease rates, most of the time due to limited or even nonexistent therapeutic options. Enterococcus faecalis is a Gram-positive anaerobic facultative bacterium witch is found in the flora of the gastrointestinal tract of humans and animals. Despite its alleged harmlessness, lately E.faecalis has been proved to be an opportunistic pathogen, representing the second and the third leading cause of hospital-acquired infections worldwide. This bacterium is being pointed as a mainly cause of endocarditis, fast progressing bacteremia may present, urinary tract infections, intra-abdominal and wounds contracted in hospitals. Its physiological characteristics allows this bacterium to survive at high temperatures, high PH values and highly concentration salts. It can also resist in hostiles environments such as malnutrition, oxidative stress and traditional cleaning technics. It feature numerous virulence factors including surface proteins, hydrolytic enzymes and ability of biofilm formation, which helps the bacterium to invade, colonize and infect the host tissue. Enterococcus faecalis displays an intrinsic resistance to some classes of antibiotics such as β-lactam, lincosamides, trimetropim-sulfamethoxazole, fluoroquinolones and low concentration of aminoglycosides. Due to its mutagenic capacity and adaptive skills, this micro-organism develops and acquires new resistances, through chromosome mutations or by genetic transferences e.g. gene vanA e vanB, witch confers resistance to Vancomycin (VRE). E. faecalis has proved a constant threat of life throughout the world, so there is an urgent need to control the spread of this bacteria by developing new therapeutic methods, having regard to the ineffectiveness of current ones and looking for new strategies inside hospitals that will reduce as much as possible the transmission of the micro-organism.
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Sumi, Jáuregui Ada Elizabeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Universidad Nacional Mayor de San Marcos. Programa Cybertesis PERÚ, 2008. http://www.cybertesis.edu.pe/sisbib/2008/sumi_ja/html/index-frames.html.

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El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes
The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments
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Böttcher, Daiana Elisabeth. "Avaliação do efeito da presença do biofilme de Enterococcus faecalis no canal radicular sobre a manutenção da substantividade da clorexidina a 2% : estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/110768.

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Introdução: O objetivo do presente estudo foi correlacionar o efeito antimicrobiano residual e a substantividade da solução de clorexidina a 2%, em dentina humana de dentes extraídos e contaminada com E. faecalis, por 48 horas, 7 e 30 dias. Metodologia: Cento e vinte e três dentes humanos extraídos foram utilizados para esse estudo. As amostras foram divididas em quatro grupos conforme a solução irrigadora utilizada (CHX 2% ou soro fisiológico) e na presença ou ausência do biofilme de Enterococcus faecalis. As amostras foram mantidas em contato com a respectiva solução durante 5 minutos. Cada grupo foi distribuído aleatoriamente em 3 subgrupos de acordo com o período de avaliação (n=10). A quantidade de CHX presente foi avaliada através de cromatografia líquida de alta eficiência (HPLC) e a viabilidade bacteriana foi analisada através de microscópio confocal a laser (CLSM). A análise estatistica foi feita através dos testes de Kruskal-Wallis e Mann-Whitney U (P<.05) e teste de correlação de Spearman (P<.01). Resultados: Houve uma correlação negativa entre o percentual de células viáveis e a quantidade de CHX remanescente (P = .000). A CHX reduziu significativamente o percentual de células viáveis em relação ao soro após 48 horas (P = .007). A diferença foi mantida no período de 7 dias (P = .001). Após 30 dias, o grupo CHX apresentou um aumento da viabilidade bacteriana tornando-se semelhante ao soro (P = .623). Simultaneamente, a quantidade de CHX reduziu significativamente após 30 dias (P = .000). Conclusão: Os resultados do presente estudo indicam que a CHX a 2% foi detectada nos periodos de 48 horas e 7 dias, mantendo reduzido o percentual de células viáveis. A presença de microrganismos na dentina humana não alterou a quantidade de CHX residual.
Introduction: The aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (CHX) on dentin by means of confocal laser scanning microscope (CLSM) and high-performance liquid chromatography (HPLC) for 48 hours, 7 and 30 days. Methods: One hundred twenty three extracted human teeth were used. Samples were divided into 4 groups according to the solution (CHX or saline) and the presence of Enterococus faecalis biofilm. Samples were kept in contact with 5mL of the solution for 5 minutes. Each group was divided into 3 subgroups according to the evaluation period (n = 10). Statistical analysis was performed by using the Kruskal- Wallis, Mann-Whitney U tests (P < .05) and Spearman’s Rank Correlation Coefficient (P < .01). Results: There was a negative correlation between the percentage of live cells and the amount of remaining CHX (P = .000). CHX significantly reduced the percentage of viable cells compared to saline after 48 hours (P = .007). Differences were maintained in the 7-day evaluation (P = .001). After 30 days, CHX group presented an increase of viable cells, thereby becoming similar to saline (P = .623). Simultaneously, remaining CHX significantly reduced in the 30-day specimens (P = .000). Conclusion: The results of this study indicate that 2% CHX solution was detected for 48 hours and 7 days, keeping a low percentage of viable cells. The presence of microorganisms on human dentin did not affect 2% chlorhexidine maintenance.
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Tse, Chee-choong Micheal, and 謝志聰. "Effect of ultrasonic agitation on enterococcus faecalis biofilm." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45165993.

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Abadía, Patiño Lorena. "Caractérisation de l'opéron vanE chez Enterococcus faecalis BM4405." Paris 7, 2003. http://www.theses.fr/2003PA077001.

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Diederich, Ann-Kristin [Verfasser], and Johannes [Akademischer Betreuer] Hübner. "Membrane lipids of Enterococcus faecalis as microbial pathogens." Freiburg : Universität, 2016. http://d-nb.info/1119452708/34.

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Sumi, Jáuregui Ada Lizbeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/907.

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El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes.
The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments.
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Neto, Manoel Matos. "Avaliação in vitro da eficácia de técnicas endodônticas de preparo mecânico na redução de Enterococcus faecalis." Universidade de Taubaté, 2007. http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=483.

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O preparo mecânico tem por objetivo a modelagem dos canais radiculares e redução de microrganismos nele presentes. Para sua execução foram utilizados instrumentos endodônticos confeccionados em aço inoxidável ou ligas de níquel-titânio. Esse estudo tem como objetivo avaliar a eficácia de três sistemas de preparo mecânico na redução da contagem de Enterococcus faecalis no interior de canais radiculares. Para isso foram utilizados 24 dentes humanos unirradiculares, divididos em três grupos: G1 - instrumentação com o sistema ProTaper rotatório, G2 - com sistema ProTaper manual e G3 - com limas tipo Kerr manuais. Os canais radiculares foram contaminados com Enterococcus faecalis, e após formação de biofilme, submetidas ao preparo mecânico. As unidades formadoras de colônia foram contadas antes e depois da instrumentação e a porcentagem de redução calculada. Os resultados demonstraram que todos os sistemas reduziram significantemente a quantidade de microrganismos dentro do canal radicular. Contudo, quando se comparou a redução promovida pelos sistemas entre si não houve diferença significativa. O estudo confirmou a eficácia do preparo mecânico, entretanto nenhum dos sistemas avaliados foi superior ao outro na redução do número de Enterococcus faecalis no interior dos canais radiculares.
The objects of mechanical preparation are to model root canals and reduce the number of microorganisms present in them. For this purpose, endodontic instruments made of stainless steel or nickel-titanium alloys are used. The aim of this study was to assess the efficacy of three systems of mechanical preparation for reducing the counting of Enterococcus faecalis inside root canals. To do this, 24 single rooted human teeth were used, divided into three groups: G1 instrumentation with the ProTaper rotary system, G2 with the manual ProTaper system and G3 manual Kerr type files. The root canals were contaminated with Enterococcus faecalis, and after biofilm had formed, they were submitted to mechanical preparation. The colony forming units were counted before and after instrumentation and the percentage of reduction was calculated. The results demonstrated that all the systems significantly reduced the number of microorganisms inside the root canal. However, when the reduction promoted by the systems was compared among them, there was no significant difference. The study confirmed the efficacy of mechanical preparation, however, none of the assessed systems was superior to the other for reducing the number of microorganisms inside root canals.
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Arruda, Theodora Thays Prado. "Perfil de sensibilidade de cepas planctÃnicas e biofilmes de enterococcus faecalis frente a desafios antimicrobianos." Universidade Federal do CearÃ, 2007. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=441.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
Enterococcus faecalis foi sugerido como sendo um importante agente etiolÃgico do insucesso endodÃntico. Foi encontrado no sistema de canais radiculares em um percentual de 22% a 77% e foi associado com a formaÃÃo de estruturas chamadas biofilmes. O objetivo do presente estudo foi avaliar, in vitro, a efetividade de cimentos endodÃnticos, hipoclorito de sÃdio a 2,5% e soluÃÃo do Ãleo essencial da Lippia sidoides a 0,5% na eliminaÃÃo de biofilmes de E. faecalis. Material clÃnico foi coletado de 37 pacientes com infecÃÃes crÃnicas do canal radicular e 14 cepas de Enterococcus faecalis foram isoladas (37,8%). Biofilmes de uma cepa de coleÃÃo de cultura (ATCC) e de uma cepa clÃnica multiresistente (Isolado 12) foram incubados por 8 dias. Esse perÃodo foi selecionado baseado em estudo cronolÃgico do desenvolvimento de biofilmes de Enterococcus faecalis atravÃs de Microscopia de ForÃa AtÃmica. Foi verificado que houve uma reduÃÃo significativa no nÃmero de bactÃrias quando os biofilmes foram expostos aos cimentos endodÃnticos em relaÃÃo ao controle para as duas cepas testadas (p<0.001). Analisando a cepa ATCC, foi verificado que o cimento endodÃntico Epiphany apresentou aÃÃo similar ao cimento Endofill (p>0.05); resultado semelhante foi encontrado para o isolado 12. Quando a susceptibilidade das cepas frente aos cimentos endodÃnticos foi comparada verificou-se que o Isolado 12 foi menos susceptÃvel comparado à cepa ATCC (p<0.001). Observou-se que a soluÃÃo do Ãleo essencial de Lippia siodides a 0,5% apresentou aÃÃo similar ao hipoclorito de sÃdio a 2,5% quando os biofilmes das duas cepas foram expostos por 10 minutos a essas substÃncias (p<0.001). Comparando a susceptibilidade das duas cepas Ãs soluÃÃes testadas, nÃo houve diferenÃa entre elas (p>0.05)
Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failure. It has been found in the root canal system in a perceptual ranging from 22% to 77% and it has been associated to organisms structured in biofilms. The aim of the present study was to evaluate, in vitro, the effectiveness of endodontic sealers, 2.5% sodium hypochlorite, and 0.5% Lippia sidoides essential oil in eliminating E. faecalis biofilms. Clinical material was collected from 37 patients with root canal chronic infections and 14 Enterococcus faecalis strains were isolated (37.8%). Biofilms from a reference (ATCC) and a clinical multirresistant strain (isolate 12 ) were grown for 8 days. This period was selected based on a chronological study of the Enterococcus faecalis biofilm development through Atomic Force Microscopy. It was verified that there was a significant reduction of the bacteria number when the biofilms were exposed to the endodontic sealers related to the control for the two tested strains (p<0.001). Analyzing the ATCC strain, it was seen that the Epiphany endodontic sealer presented similar action compared to Endofill (p>0.05), similar result was found for Isolate 12. When the strains susceptibilities against the sealers was compared it was verified that the isolate 12 was less susceptible than the ATCC strain (p<0.001). It was verified that the 0.5% essential oil solution of Lippia siodides presented a similar action to 2.5% sodium hypochlorite when the biofilm of ATCC strain and isolate 12 were exposed for 10 minutes to this substances (p<0.001). Comparing the susceptibility of the two strains to the solutions tested, there was no difference between them (p>0.05)
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Books on the topic "Enterococcus faecalis"

1

Cattelan, Jessy. Immunologische Studie zum Oberflächenprotein TraM, ein Typ IV Sekretionsprotein in Enterococcus faecalis / Enterococcus faecium. [S.l: s.n.], 2014.

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Berthold, Martin. Die Pathogenität biofilmassoziierter Virulenzfaktoren von Enterococcus faecalis im Endokarditismodell. [S.l: s.n.], 2014.

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Shorrock, Patricia Joan. Surface properties of enterococcus faecalis in relation to infective endocarditis. Birmingham: Aston University. Department of Pharmaceutical Sciences, 1990.

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Bao, Yinyin. Role of mprF1 and mprF2 in the pathogenicity of Enterococcus faecalis. Freiburg: Universität, 2012.

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Peters, Tansy M. Studies on the activation of azo-dyes into direct-acting genotoxic agents by enterococcus faecalis. London: University of East London, 1995.

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Mack, Henry L. Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects. Nova Science Publishers, Incorporated, 2014.

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Bambawale, Amrut, Sumita Bhagwat, and Leena Padhye. Aktywność przeciwdrobnoustrojowa Propolis, HEBP, chlorheksydyny, EDTA: Przeciwko Enterococcus Faecalis i Candida Albicans ; badanie Invitro. Wydawnictwo Nasza Wiedza, 2020.

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Shepard, Brett David. Differential expression of virulence-related genes by Enterococcus faecalis in response to biological cues. [s.n.], 2002.

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Herrington, William G., Aron Chakera, and Christopher A. O’Callaghan. Urinary tract infection. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0158.

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A urinary tract infection (UTI) is defined as present when urine culture results in the growth of a single organism at greater than 105 colony-forming units/ml of urine. Bowel flora are the commonest cause of UTIs. Escherichia coli accounts for 80% of infections. Klebsiella spp., Proteus mirabilis, Enterococcus faecalis, and Staphylococcal saprophyticus account for most of the remaining 20%. Staphylcoccus aureus culture is usually catheter related or secondary to haematogenous spread. This chapter looks at the symptoms of a UTI, as well as its demographics, complications, diagnosis (including investigations), and treatment. It also discusses prognosis.
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Gina, Pugliese, and Weinstein Robert A. 1946-, eds. Issues & controversies in prevention and control of VRE, vancomycin-resistant enterococci. Chicago: Etna Communications, 1998.

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Book chapters on the topic "Enterococcus faecalis"

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Murray, Barbara E., Liangxia Jiang, Jianguo Xiao, Xiang Qin, Kavindra V. Singh, Aart de Kok, Al Claiborne, Patrice Courvalin, and George M. Weinstock. "Enterococcus faecalis." In Bacterial Genomes, 649–50. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_59.

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Manson, Janet M., and Michael S. Gilmore. "Pathogenomics of Enterococcus faecalis." In Pathogenomics, 125–47. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2006. http://dx.doi.org/10.1002/352760801x.ch7.

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Wirth, Reinhard. "The sex pheromone system of Enterococcus faecalis." In EJB Reviews 1994, 117–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-79502-2_9.

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McShan, W. Michael, and Nathan Shankar. "The Genome of Enterococcus faecalis V583: a Tool for Discovery." In The Enterococci, 409–15. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817923.ch11.

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Fujimoto, S., M. Bastos, K. Tanimoto, F. An, K. Wu, and D. B. Clewell. "The pAD1 Sex Pheromone Response in Enterococcus faecalis." In Streptococci and the Host, 1037–40. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_244.

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Clermont, Dominique, Gilda de Cespédès, Françoise Delbos, and Théa Horaud. "Genetic Analysis of Enterococcus faecalis Chromosome Carrying Mobile Elements." In Streptococci and the Host, 1023–27. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_241.

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Shankar, Viswanathan, and Michael S. Gilmore. "Characterization of the Enterococcus faecalis Alpha C Protein Homolog." In Streptococci and the Host, 1045–48. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_246.

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Nakayama, Jiro, Yuuichiro Takanami, and Akinori Suzuki. "Analysis of Pheromone Binding and Pheromone Reception by Enterococcus faecalis." In Streptococci and the Host, 1033–35. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_243.

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Pasquarella, Cesira, Donald Morrison, Angelo Savino, and Barry D. Cookson. "Dynamics of Enterococcus faecalis Colonization of Bone Marrow Transplant Patients." In Streptococci and the Host, 275–79. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_68.

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Clewell, Don B. "Sex Pheromones and the Plasmid-Encoded Mating Response in Enterococcus faecalis." In Bacterial Conjugation, 349–67. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_14.

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Conference papers on the topic "Enterococcus faecalis"

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Keyhani, Jacqueline, and Ezzatollah Keyhani. "Increased resistance to detergent in Enterococcus faecalis." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0010.

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Santos, Pâmela Gomes, Rosane Nassar Meireles Guerra, Josivan Regis Farias, Simone Batista Muniz, and Danielle Cristine Gomes Franco. "AÇÃO ANTIMICROBIANA DAS FLORES DE ANACARDIUM OCCIDENTALE E DO ÁCIDO ELÁGICO PRESENTE NO EXTRATO." In I Congresso Brasileiro de Imunologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/965.

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Introdução: As infecções bacterianas têm aumentado significativamente nas últimas décadas, sobretudo aquelas ocasionadas por microrganismos multirresistentes. Assim, o uso de produtos naturais com finalidades terapêuticas surge com alvo de bioprospecção na busca de novos compostos com ação antimicrobiana. Além disso, o uso de insetos, como o Tenebrio molitor como modelo experimental para avaliação in vivo tem sido muito frequente, pois exige menos material em relação aos testes com animais vertebrados. Objetivo: O presente trabalho investigou o efeito citotóxico e ação antimicrobiana do extrato hidroalcoólico das flores de Anacardium occidentale (EHAo) e do ácido elágico. Material e Métodos: Avaliamos a citoxicidade de ácido elágico e do EHAo nas concentrações (1; 5 e 50mg/kg) em Tenebrio molitor. A ação antimicrobiana para Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae e a toxicidade do ácido elágico, foi avaliada por microdiluição, segundo a norma M7-A6 do manual da Clinical and Laboratory Standards Institute – CLSI. Foi determinada a Concentração Bactericida Mínima (CBM) e concentração inibitória mínima (CIM), em culturas de 24 horas, incubadas à 37ºC. Resultados: No ensaio de citotoxidade aguda se verificou que nenhuma das concentrações usados foram tóxicas, pois não ocorreram óbitos e nem nenhuma anormalia morfológica nas larvas de Tenebrio molitor. Os testes de concentração inibitória mínima (CIM) de concentração bactericida mínima (CBM) mostraram que o EHAo apresentou ação bactericida para Enterococcus faecalis em todas as concentrações testadas. Para Staphylococcus aureus os resultados mostraram ação bactericida para as maiores concentrações e bacteriostática para a menor diluição. O ácido elágico teve ação bactericida apenas para Enterococcus faecalis. Para as bactérias Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae as concentrações de EHAo e ácido elágico testadas não foram inibitórias. Conclusões: Os resultados mostraram baixa toxicidade tanto para o EHAo como para o ácido elágico e ainda, que o extrato apresentou melhor efeito antimicrobiano do que o ácido elágico, para Enterococcus faecalis e Staphylococcus aureus.
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SILVA, MARIA EDUARDA ROCHA JACQUES DA, GABRIELA MERKER BREYER, ANA PAULA GUEDES FRAZZON, and FRANCIELE MABONI SIQUEIRA. "FORMAÇÃO DE BIOFILME POR ENTEROCOCCUS FAECALIS ISOLADOS DE FELINOS DOMÉSTICOS." In III Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2023. http://dx.doi.org/10.51161/iii-conamic/16170.

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Du, Juan, Hofung Cheng, Ken T. M. Wong, and Stanley C. K. Lau. "A microcosm study on the die-off response of the indicator bacteria, Enterococcus faecium and Enterococcus faecalis." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0001.

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Tverezovska, Olesia, Viktoriia Holubnycha, Rafal Banasiuk, Yevheniia Husak, Savchenko Anton, and Viktoriia Korniienko. "The Effect of Silver Nanoparticles Against Formation of Enterococcus Faecalis Biofilms." In 2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP). IEEE, 2022. http://dx.doi.org/10.1109/nap55339.2022.9934155.

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Kovalenko, I., K. Golubykh, M. Malik, C. Shah, A. Amirian, and J. Goldman. "Enterococcus Faecalis Bacteremia: The Driving Force Behind Septic Thrombophlebitis and Cavitary Pneumonia." In American Thoracic Society 2023 International Conference, May 19-24, 2023 - Washington, DC. American Thoracic Society, 2023. http://dx.doi.org/10.1164/ajrccm-conference.2023.207.1_meetingabstracts.a5343.

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LUIS BAPTISTA DE GUSMÃO, PEDRO, THAIS DA SILVA FEITOSA, Brenda Paula Figueiredo de Almeida Gomes, Eloá Cristina Bícego-Pereira, and MAICON RICARDO ZIEBERG PASSINI. "Isolamento e identificação de Enterococcus faecalis de dentes submetidos à reintervenção endodôntica." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-51716.

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Fernandes, Meg da Silva, Luciana Maria Ramires Esper, Dirce Yorika Kabuki, and Arnaldo Yoshiteru Kuaye. "Produção de Moléculas Sinalizadoras de Quorum Sensing por Cepas de Enterococcus faecium e Enterococcus faecalis Isoladas do Processamento de Ricota." In V Simpósio de Bioquímica e Biotecnologia. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/biochem-vsimbbtec-21938.

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Ke Sun, Jue Zhang, Jing Fang, Jing Wang, Jie Pan, and Weidong Zhu. "Cold plasma therapy for enterococcus faecalis biofilm infected tooth root canal in vitro." In 2012 IEEE 39th International Conference on Plasma Sciences (ICOPS). IEEE, 2012. http://dx.doi.org/10.1109/plasma.2012.6383868.

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Paula Figueiredo De Almeida Gomes, Brenda, Maikon Tadeu Ferrari Martinucho, Ela͍se Gabriele Martins, and Beatriz Leonardo Prudenciano. "Avaliação de substâncias químicas auxiliares, utilizadas em endodontia, na redução de Enterococcus faecalis." In XXIII Congresso de Iniciação Científica da Unicamp. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.19146/pibic-2015-38003.

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Reports on the topic "Enterococcus faecalis"

1

A. Komnos, Georgios, Antonios Papadopoulos, Efstratios Athanaselis, Theofilos Karachalios, and Sokratis E. Varitimidis. Migrating Periprosthetic Infection from a Total Hip Replacement to a Contralateral Non-Operated Osteoarthritic Knee Joint. Science Repository, January 2023. http://dx.doi.org/10.31487/j.ijscr.2022.03.02.

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Introduction: There is a paucity of published data on whether a treated infected arthroplasty is a risk factor for infection in another, non-operated joint. Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty is a relatively rare entity. Case: We report a case of migration of a pathogen (Enterococcus faecalis) from an infected prosthetic joint (hip) to the contralateral native joint (knee). Identification of the pathogen was made with PCR, by obtaining cultures during the implantation of the primary knee prosthesis. Conclusion: Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty has not been widely reported. Management of such cases is extremely challenging and without clear and established guidelines. Our experience shows that tissue samples should be taken intraoperatively and sent for cultures, so as to exclude contamination in those cases.
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Bezerra, Alexandre Sacchetti, Flavia Altheman Loureiro, Carla Maria Pasquareli Vazquez, Afonso Cesar Polimanti, and Rafi Felicio Bauab Dauar. Empiric Treatment of Foot Infection in Patients with Severe Diabetes. Science Repository, December 2021. http://dx.doi.org/10.31487/j.jicoa.2021.04.04.

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Background: Despite being treated with antibiotics of broad spectrum recommended by International Consensus, severe diabetic patients with lower limb infection do not present a positive clinical evolution during empirical treatment. This study’s bacterial profile was analysed and compared with other worldwide hospital centers. Objective: To confirm the need of an individualized empirical treatment for severe diabetic patients with foot infection. Methods: Retrospective analysis of cultures and antibiograms of severe diabetic patients admitted by foot infection. Results: The results were consistent with the socioeconomic realities of developing countries. Gram-negative bacteria (52,11%) were present in most bone cultures. Results presented a high incidence of Enterococcus faecalis in both gram-positive (21,2%) and polymicrobial (34,7%) samples. Bacterial resistance with the use of ordinary antibiotics in the statistical analysis was high. Conclusion: The community infections should undergo broad spectrum empirical therapy combining amikacin (80,43%) or meropenem (72,00%) with gram-negative and vancomycin (100%) or teicoplanin (90,00%) or linezolid (74,19%) with gram-positive.
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