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1

Hu, Wei-Shou. "Cell culture engineering." Trends in Biotechnology 6, no. 5 (May 1988): 83–84. http://dx.doi.org/10.1016/0167-7799(88)90061-3.

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KISS, R. "Cell culture engineering." Trends in Biotechnology 14, no. 6 (June 1996): 179–81. http://dx.doi.org/10.1016/0167-7799(96)30010-3.

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3

Ulloa‐Montoya, Fernando, Gargi Seth, Catherine M. Verfaillie, and Wei‐Shou Hu. "Stem cell culture engineering." Journal of the Chinese Institute of Engineers 28, no. 7 (October 2005): 1039–52. http://dx.doi.org/10.1080/02533839.2005.9671081.

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4

Aunins, J., M. Betenbaugh, and J. Aunins. "Cell Culture Engineering VIII." Biotechnology Progress 19, no. 1 (February 7, 2003): 1. http://dx.doi.org/10.1021/bp020149+.

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5

FUKUDA, Shuichi. "Japanese Culture and Engineering." Proceedings of Design & Systems Conference 2017.27 (2017): 2509. http://dx.doi.org/10.1299/jsmedsd.2017.27.2509.

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Brown, Alan S. "Penetrating the Engineering Culture." Mechanical Engineering 134, no. 10 (October 1, 2012): 42–45. http://dx.doi.org/10.1115/1.2012-oct-3.

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This article discusses ASME/Autodesk Sustainable Design Survey results and suggestions. The survey reveals that more engineers than ever before report working on an increasingly diverse range of sustainability projects. Companies are also showing growing interest in using recycled and renewable materials, and minimizing toxic and other substances of concern. The survey asked engineers to pick the two most important sustainable practices. However, several engineers used the survey to complain about government regulations. Suggestions provided by engineers and experts ranged from offering more college and on-the-job training courses in sustainability to sharing best practices and showcasing successful designs. Several engineers wanted a set of standards—definitions and measurements—to design against. The survey suggests that sustainable practices involve a change in mindset that is difficult to implement. Innovation always contains a certain element of risk, and minimizing risk is always at the forefront in business practice.
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7

Zeng, Prof Dr An-Ping, and Prof Dr Ing Ralf Pörtner. "Editorial: Cell Culture Engineering." Engineering in Life Sciences 15, no. 5 (July 2015): 457–58. http://dx.doi.org/10.1002/elsc.201570053.

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8

Prewitz, Marina, Friedrich Philipp Seib, Martin Bornhaeuser, and Carsten Werner. "Engineering Biomimetic Culture Systems: Impact On Human Bone Marrow-Derived Stem Cells." Blood 114, no. 22 (November 20, 2009): 3628. http://dx.doi.org/10.1182/blood.v114.22.3628.3628.

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Abstract Abstract 3628 Poster Board III-564 The bone marrow (BM) harbours haematopoietic stem/progenitor cells (HSCs) in anatomically distinct sites (niches) where HSCs are subjected to regulatory cues such as cytokines, cell-cell contacts and extra-cellular matrix (ECM) all of which control stem cell fate. In particular mesenchymal stromal cells (MSCs) are an integral part of the bone marrow and are known to be key regulators of the HSC niche. We have previously shown that bio-artificial scaffolds can have a significant impact on the in vitro behaviour of MSCs. Here, we are therefore focussing on the role of (native) ECM within the MSC-HSC microenvironment by building on our previous findings and published data (Seib et al.,Tissue Eng Part A., 2009 in press). Thus the aim of the current study is (a) to identify niche-specific ECM components and (b) the use of such ECMs for in vitro culture of BM-derived stem cells. To mimic the natural ECM composition of the BM, different ECM types were generated from BM-derived cells using (a) Dexter cultures, (b) standard MSC cultures, (c) MSCs subjected to osteogenic differentiation. After 10 days of culture those MSC-derived ECMs were decellularised using 0.5% Triton-X and 20mM NH4OH leaving only the ECM behind (verified by scanning electron microscopy). Those ECMs were used as a substrate for a second culture of MSCs, which were analysed for their proliferation and differentiation potential. Cell-free ECM from standard MSC cultures improved MSC proliferation compared to cells grown on regular tissue culture plastic (TCP) over the period of 8 days. Most notably, all cell-free ECM preparations lead to a significant difference in the cytoskeletal arrangement of MSCs during the first 2 days of culture compared to TCP controls. Cultivation of MSCs on native ECM provided a guiding structure for those cells to grow into, and helped to maintain an elongated cell shape compared to substantial cell spreading on TCP (roundness 0.2 versus 0.5 and cell area of 2.2 versus 8.2mm2, respectively, p<0.001, n=60. A factor of 1 was set to equate to a perfect circle). Next, we investigate if native ECM could either directly improve HSC cultures or maximise MSC feeder characteristics. For the latter set of studies MSCs were initially cultured for 7 days on cell-free ECM (from standard MSC cultures) and subsequently co-cultured with human peripheral blood CD34+ HSCs in serum free medium supplemented with cytokines (Tpo, Flt3, and SCF at 10ng/ml). Following a 14 day culture period up to 3.5-fold more CD34+ cells were present in ECM co-cultures compared to TCP co-cultures that was accompanied with an overall expansion of CD45+ cells of 109-fold versus 35-fold, respectively. Our data suggest that ECM preparations derived from MSCs might be useful to accomplish better expansion of HSCs under defined culture conditions. In addition, this system permits the identification of bimolecular key components that can be utilized in the future design of simple and robust carrier systems for improved HSC maintenance in vitro. Figure HSC-MSC co-culture on preformed ECM substrates. (A) MSC-derived ECM (from standard MSC culture) following cell lysis (complete absence of cells). (B) Growth of a new set of MSCs on ECM substrates as shown in (A). (C) HSC-MSC co-culture on ECM substrates. Scale bars at 2μm. Arrow heads point out ECM structures. Figure HSC-MSC co-culture on preformed ECM substrates. (A) MSC-derived ECM (from standard MSC culture) following cell lysis (complete absence of cells). (B) Growth of a new set of MSCs on ECM substrates as shown in (A). (C) HSC-MSC co-culture on ECM substrates. Scale bars at 2μm. Arrow heads point out ECM structures. Disclosures: No relevant conflicts of interest to declare.
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9

Villanueva Alarcón, Idalis, Robert Jamaal Downey, Louis Nadelson, Jana Bouwma-Gearhart, and YoonHa Choi. "Light Blue Walls and Tan Flooring: A Culture of Belonging in Engineering Making Spaces (or Not?)." Education Sciences 11, no. 9 (September 18, 2021): 559. http://dx.doi.org/10.3390/educsci11090559.

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The motivation for this exploratory qualitative study is to understand what a culture of belonging may look like across six engineering education making spaces in institutions of higher education in the U.S. The research question for this study was: In what ways are the management, instructors, and staff operating engineering education making spaces influencing a culture of belonging (if any) for engineering students? We examined the transcripts of semi-structured interviews of 49 faculty members and 29 members of management/staff of making spaces, using thematic coding. From the data, we identified four themes that described the culture of belonging being created in these six engineering making spaces: (a) a ‘closed loop’ culture for inclusion, diversity, equity, and access; (b) a ‘transactional, dichotomous’ culture; (c) a ‘band-aid, masquerading’ culture; (d) a potential ‘boundary-crossing’ culture. Our primary conclusion was that created cultures in engineering making spaces are extensions of normative cultures found in traditional engineering classrooms. Additionally, while making spaces were attempting to change this culture in their physical infrastructures, it was deemed that the space leadership needs to expand hiring strategies, the nature of making activities, the ambient/physical appearance of the space, disciplines, and required expertise, to create a truly inclusive and equitable culture of belonging.
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DU, Dajiang, Shunsuke MIYAUCHI, Katsuko FURUKAWA, Kohei TSUCHIYA, and Takashi USHIDA. "10204 OSCILLATORY PERFUSION SEEDING AND CULTURE FOR BONE TISSUE ENGINEERING." Proceedings of Conference of Kanto Branch 2006.12 (2006): 327–28. http://dx.doi.org/10.1299/jsmekanto.2006.12.327.

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11

Radisic, M., H. Park, S. Gerecht, C. Cannizzaro, R. Langer, and G. Vunjak-Novakovic. "Biomimetic approach to cardiac tissue engineering." Philosophical Transactions of the Royal Society B: Biological Sciences 362, no. 1484 (June 26, 2007): 1357–68. http://dx.doi.org/10.1098/rstb.2007.2121.

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Here, we review an approach to tissue engineering of functional myocardium that is biomimetic in nature, as it involves the use of culture systems designed to recapitulate some aspects of the actual in vivo environment. To mimic the capillary network, subpopulations of neonatal rat heart cells were cultured on a highly porous elastomer scaffold with a parallel array of channels perfused with culture medium. To mimic oxygen supply by haemoglobin, the culture medium was supplemented with a perfluorocarbon (PFC) emulsion. Constructs cultivated in the presence of PFC contained higher amounts of DNA and cardiac markers and had significantly better contractile properties than control constructs cultured without PFC. To induce synchronous contractions of cultured constructs, electrical signals mimicking those in native heart were applied. Over only 8 days of cultivation, electrical stimulation induced cell alignment and coupling, markedly increased the amplitude of synchronous construct contractions and resulted in a remarkable level of ultrastructural organization. The biomimetic approach is discussed in the overall context of cardiac tissue engineering, and the possibility to engineer functional human cardiac grafts based on human stem cells.
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Quintero-Bermudez, Rafael, Rosa Maria Bermudez-Cruz, and Rafael Quintero-Torres. "Science, engineering, technology, and culture." IEEE Potentials 41, no. 5 (September 2022): 15–19. http://dx.doi.org/10.1109/mpot.2016.2594701.

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13

Sharp, H., H. Robinson, and M. Woodman. "Software engineering: community and culture." IEEE Software 17, no. 1 (2000): 40–47. http://dx.doi.org/10.1109/52.819967.

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14

TAKEMURA, Kenjiro, and Yuta KURASHINA. "Informotion for Cell Culture Engineering." Journal of the Japan Society of Applied Electromagnetics and Mechanics 27, no. 4 (2019): 402–6. http://dx.doi.org/10.14243/jsaem.27.402.

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15

Wlaschin, Katie F., Gargi Seth, and Wei-Shou Hu. "Toward genomic cell culture engineering." Cytotechnology 50, no. 1-3 (March 2006): 121–40. http://dx.doi.org/10.1007/s10616-006-9004-9.

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16

Kosmala, Peter B. "Engineering a Culture of Privacy." IEEE Consumer Electronics Magazine 9, no. 2 (March 1, 2020): 83–88. http://dx.doi.org/10.1109/mce.2019.2954562.

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17

Eswaramoorthy, Sindhuja D., Nandini Dhiman, Gayathri Korra, Carlo M. Oranges, Dirk J. Schaefer, Subha N. Rath, and Srinivas Madduri. "Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold." Regenerative Medicine 14, no. 7 (July 2019): 647–61. http://dx.doi.org/10.2217/rme-2018-0166.

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Aim: We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs co-culture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds. Methods: The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies. Results: The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression. Conclusion: These findings suggest that iECs mimic endothelial cells when co-cultured with MSCs and that one MSCs source can be used to give rise to both MSCs and iECs. The isogenic MSCs/iECs co-culture provides a new option for bone tissue engineering applications.
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18

Jia, Zhidong, Yuan Cheng, Xinan Jiang, Chengyan Zhang, Gaoshang Wang, Jiecheng Xu, Yang Li, Qing Peng, and Yi Gao. "3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity." BioMed Research International 2020 (March 4, 2020): 1–22. http://dx.doi.org/10.1155/2020/6354183.

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In vitro 3D hepatocyte culture constitutes a core aspect of liver tissue engineering. However, conventional 3D cultures are unable to maintain hepatocyte polarity, functional phenotype, or viability. Here, we employed microfluidic chip technology combined with natural alginate hydrogels to construct 3D liver tissues mimicking hepatic plates. We comprehensively evaluated cultured hepatocyte viability, function, and polarity. Transcriptome sequencing was used to analyze changes in hepatocyte polarity pathways. The data indicate that, as culture duration increases, the viability, function, polarity, mRNA expression, and ultrastructure of the hepatic plate mimetic 3D hepatocytes are enhanced. Furthermore, hepatic plate mimetic 3D cultures can promote changes in the bile secretion pathway via effector mechanisms associated with nuclear receptors, bile uptake, and efflux transporters. This study provides a scientific basis and strong evidence for the physiological structures of bionic livers prepared using 3D cultures. The systems and cultured liver tissues described here may serve as a better in vitro 3D culture platform and basic unit for varied applications, including drug development, hepatocyte polarity research, bioartificial liver bioreactor design, and tissue and organ construction for liver tissue engineering or cholestatic liver injury.
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19

Radisic, Milica, and Gordana Vunjak-Novakovic. "Cardiac tissue engineering." Journal of the Serbian Chemical Society 70, no. 3 (2005): 541–56. http://dx.doi.org/10.2298/jsc0503541r.

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We hypothesized that clinically sized (1-5 mm thick),compact cardiac constructs containing physiologically high density of viable cells (?108 cells/cm3) can be engineered in vitro by using biomimetic culture systems capable of providing oxygen transport and electrical stimulation, designed to mimic those in native heart. This hypothesis was tested by culturing rat heart cells on polymer scaffolds, either with perfusion of culture medium (physiologic interstitial velocity, supplementation of per fluorocarbons), or with electrical stimulation (continuous application of biphasic pulses, 2 ms, 5 V, 1 Hz). Tissue constructs cultured without perfusion or electrical stimulation served as controls. Medium perfusion and addition of per fluorocarbons resulted in compact, thick constructs containing physiologic density of viable, electromechanically coupled cells, in contrast to control constructs which had only a?100 ?m thick peripheral region with functionally connected cells. Electrical stimulation of cultured constructs resulted in markedly improved contractile properties, increased amounts of cardiac proteins, and remarkably well developed ultrastructure (similar to that of native heart) as compared to non-stimulated controls. We discuss here the state of the art of cardiac tissue engineering, in light of the biomimetic approach that reproduces in vitro some of the conditions present during normal tissue development.
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Atadero, Rebecca A., Christina H. Paguyo, Karen E. Rambo-Hernandez, and Heather L. Henderson. "Building inclusive engineering identities: implications for changing engineering culture." European Journal of Engineering Education 43, no. 3 (November 6, 2017): 378–98. http://dx.doi.org/10.1080/03043797.2017.1396287.

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Wightman, Raymond, and C. J. Luo. "From mammalian tissue engineering to 3D plant cell culture." Biochemist 38, no. 4 (August 1, 2016): 32–35. http://dx.doi.org/10.1042/bio03804032.

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Recent advances applying mammalian tissue engineering to in vitro plant cell culture have successfully cultured single plant cells in a 3D microstructure, leading to the discovery of plant cell behaviours that were previously not envisaged. Animal and plant cells share a number of properties that rely on a hierarchical microenvironment for creating complex tissues. Both mammalian tissue engineering and 3D plant culture employ tailored scaffolds that alter a cell's behaviour from the initial culture used for seeding. For humans, these techniques are revolutionizing healthcare strategies, particularly in regenerative medicine and cancer studies. For plants, we predict applications both in fundamental research to study morphogenesis and for synthetic biology in the agri-biotech sector.
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Tang, S. L., Raymond T. M. Aoieong, and Candy S. L. Tsui. "Quality Culture Auditing for Engineering Consultants." Journal of Management in Engineering 25, no. 4 (October 2009): 204–13. http://dx.doi.org/10.1061/(asce)0742-597x(2009)25:4(204).

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Cech, Erin A. "Culture of Disengagement in Engineering Education?" Science, Technology, & Human Values 39, no. 1 (September 13, 2013): 42–72. http://dx.doi.org/10.1177/0162243913504305.

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Johns, Nick, and John Chesterton. "ICL Kidsgrove: Engineering a Quality Culture." International Journal of Contemporary Hospitality Management 6, no. 1/2 (February 1994): 25–29. http://dx.doi.org/10.1108/09596119410052053.

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Ednie-Brown, Pia, and Alisa Andrasek. "CONTINUUM: A Self-Engineering Creature-Culture." Architectural Design 76, no. 5 (2006): 18–25. http://dx.doi.org/10.1002/ad.316.

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Giot, Michel. "Loss prevention, engineering education and culture." Journal of Loss Prevention in the Process Industries 2, no. 4 (January 1989): 186. http://dx.doi.org/10.1016/0950-4230(89)80031-2.

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Hendriks, Jeanine, Jens Riesle, and Clemens A. van Blitterswijk. "Co-culture in cartilage tissue engineering." Journal of Tissue Engineering and Regenerative Medicine 1, no. 3 (2007): 170–78. http://dx.doi.org/10.1002/term.19.

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Romeo, Jim. "3D Printing: Reshaping our Engineering Culture?" Plastics Engineering 76, no. 3 (March 2020): 42–45. http://dx.doi.org/10.1002/peng.20274.

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Panwar, Amit, Prativa Das, and Lay Poh Tan. "3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering." Bioengineering 8, no. 11 (November 14, 2021): 185. http://dx.doi.org/10.3390/bioengineering8110185.

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Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.
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Borzenok, Sergey A., Boris E. Malyugin, Maxim Y. Gerasimov, Dmitriy S. Ostrovskiy, and Anna V. Shatskikh. "Feeder-Free Cell Culture of Labial Oral Mucosal Epithelium for Tissue-Engineering and Regenerative Medicine." Annals of the Russian academy of medical sciences 75, no. 5 (December 27, 2020): 561–70. http://dx.doi.org/10.15690/vramn1357.

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Background.The cultured cheek mucosa epithelium (buccal epithelium, BE) is used for autologous transplants generation and tissue engineering. An alternative source of cells for these purposes may be the lip mucosa, covered, like BE, with non-keratinized stratified squamous epithelium, but with some histological distinctions.Aims to characterize the human lip mucosa as a promising source of epithelial cells for autologous transplantation and tissue engineering.Methods.Scrapings of the lip, cheek, and gum mucosa from five healthy volunteers were analyzed by cytofluorimetry to determine the level of desquamation and cytokeratin (CK) 10 and 13 expression. The lip mucosa of two patients was characterized using routine histological staining and fluorescence immunohistochemistry for CK 3, 4, 10, 13, and p63 marker. 35 samples of full-thickness strips of the patients lip mucosa were used to set the explant (n=18) and enzymatic (n=17) techniques for expansion epithelium. Culture systems with 1.05 and 0.06mM Calcium contained 5% fetal bovine serum, 5 g/ml human insulin, 5 g/ml hydrocortisone, 10 ng/ml human epidermal growth factor. Stable cultures were stained for p63, vimentin, zonula occludens-1 (ZO-1), and CK10. Software tools determined levels of their expression.Results.The number of cells in the lip and gum samples was significantly lower than from the cheek. The median number of CK13 positive cells was significantly different for the gum (6.4%) and cheek (64.8%, p=0.0089). Significant differences for CK10 positive cells were not observed. The epithelium of the lip mucosa was 72.13.6 m thick, relatively flat, and without keratinization sites. Samples were positively stained for CK 4 and 13, in the absence of expression of CK 3 and 10. The primary culture of epithelial cells obtained by explant technique was significantly more effective (p=0.001) in comparison with the enzymatic method. Stable cultures had a cobble-stone morphology in both culture systems. The levels of vimentin and p63 expression in both culture systems was not significantly differ. ZO-1 expression was 3.6-fold higher for 1.05-mMCa++medium (p=0.0006).Conclusions.Epithelium cell culture from the lip mucosa can be obtained by culturing explants without a feeder layer. Quality control steps have been developed for cultured cells and biopsy site.
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SHIOYA, Tetsuo. "Agricultural Engineering as a Culture, and Culturization of Agricultural Engineering." Japanese Journal of Farm Work Research 31, no. 3 (1996): 215–19. http://dx.doi.org/10.4035/jsfwr.31.215.

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32

Zhang, Haoran, and Xiaonan Wang. "Modular co-culture engineering, a new approach for metabolic engineering." Metabolic Engineering 37 (September 2016): 114–21. http://dx.doi.org/10.1016/j.ymben.2016.05.007.

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Graeff, Erhardt, and Alison Wood. "Undergraduate Engineering as Civic Professionalism." Good Society 30, no. 1-2 (December 1, 2021): 76–95. http://dx.doi.org/10.5325/goodsociety.30.1-2.0076.

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Abstract Undergraduate engineering education is not doing enough to address engineering’s culture of disengagement—a culture that inhibits modern society’s ability to serve the public interest and mitigate the threat of technologies amplifying harm. We argue for visions of undergraduate engineering that purposefully embrace the humanities and make civic education integral in order to educate engineers as civic professionals. Two case studies from our college, one curricular and one extracurricular, illustrate how we are building toward a new vision by offering learning experiences in which students can evolve their personal and professional commitments to the common good and practice technical skills in ways responsible to democracy and society.
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Fahrullah. "The Effect of Organizational Culture on Production Department Employee Performance at PT. Shinkobe Engineering." Formosa Journal of Science and Technology 1, no. 8 (December 21, 2022): 1081–100. http://dx.doi.org/10.55927/fjst.v1i8.2089.

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This study aims to examine the effect of organizational culture on the performance of employees of PT Shinkobe Engineering. Data collection was carried out by distributing questionnaires. The results are shown by looking at the tcount of the organizational culture variable of 7.809 which is greater than the ttable of 1.999 with a significance level of 0.000, the significance level is lower than 0.05. Research also shows that there is a positive and significant influence given by organizational culture which has a strong influence in influencing PT Shinkobe Engineering's employee performance optimally, the resulting R value (correlation) is 0.618 so it can be said that organizational culture and performance are positively related. While the coefficient of determination R2 (R Square) is 0.381, which means that the ability of organizational culture variables to influence employee performance at PT Shinkobe Engineering is 38.1%, while 61.9% is contributed by other factors not observed in this study.
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Kuribayashi-Shigetomi, Kaori, and He Qian. "GS5-6 3D co-culture system using cell origami technique(GS5: Tissue Engineering)." Proceedings of the Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics 2015.8 (2015): 169. http://dx.doi.org/10.1299/jsmeapbio.2015.8.169.

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Xu, Hui Rong, Shao Chun Sui, Xiao Hong Wang, Yong Nian Yan, and Ren Ji Zhang. "A Bioreactor for a Pulsatile Circulatory Culture System." Advanced Materials Research 97-101 (March 2010): 1952–55. http://dx.doi.org/10.4028/www.scientific.net/amr.97-101.1952.

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Bioreactor technology is a branched research area of tissue engineering. Dynamic culture environment mimicking in vivo pulsatile conditions could be achieved by bioreactor otherwise might not through static cultures. In this paper, we present a new type of pulse bioreactor which can provide arbitrary and easily adjustable circulatory flow conditions of 0 - 0.2 MPa pressure. The pulse amplitude range was 0 - 7%. The pulse frequency can be adjusted between 0 and 80 times/min. In addition, the new type of pulse bioreactor can be sterilized and dismantled easily. This bioreactor has been used in dynamic culture of assembled adipose derived stem cells (ADSCs) and shown promise in tissue engineering.
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Rad, S., M. Normahira, and M. N. Anas. "Development of Bioreactor System for Generating Three-Dimensional (3D) Tissue Engineering." Advanced Materials Research 626 (December 2012): 902–7. http://dx.doi.org/10.4028/www.scientific.net/amr.626.902.

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In this study, perfusion bioreactor has been employed for generating a three dimensional (3D) tissue engineering. In flow perfusion culture, the culture medium is forced through the internal porous network of the scaffold. This can mitigate internal diffusional limitations present in 3D scaffold to enhance nutrient delivery and waste removal from the cultured cells. In order to validate this design, a fluid flow analysis has been conducted to show that it has a uniform flow distribution value for cell cultured conditions. This bioreactor system also equip with the temperature controller system to ensure the bioreactor temperature is always at 37°C in order to mimic human body temperature.
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Heo, Jeong Hyun, Dongyun Kang, Seung Ju Seo, and Yoonhee Jin. "Engineering the Extracellular Matrix for Organoid Culture." International Journal of Stem Cells 15, no. 1 (February 28, 2022): 60–69. http://dx.doi.org/10.15283/ijsc21190.

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Wang, Zhi Yong. "Modern Design: Industrial Technology, Engineering and Culture." Advanced Materials Research 655-657 (January 2013): 2065–68. http://dx.doi.org/10.4028/www.scientific.net/amr.655-657.2065.

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The industrialisation and modern civilisation were ever being the landmark signs for European social modernisation. Its birth strongly suggested a series cause of remarkable social and historical change, and the reasons of modern design arise as well. Under such a view, it was revealed that the industrial manufacturing and technology became the first reason of modern design and presented a basic support for design’s modernisation. Meanwhile, the progress of industrial engineering gave a further effect on the development of modern design, where the progress in public architecture and civil engineering created new standards of structure, materials as well as of new aesthetic styles for design. Moreover, modern design also showed the relationship with European social systems and culture, for the latter, as the institutional or cultural factors, dominated the functions of technology and engineering practice to modern design movements.
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MIYA, Kenzo. "Establishment of Maintenance Engineering and Safety Culture." Journal of the Society of Mechanical Engineers 109, no. 1048 (2006): 144–45. http://dx.doi.org/10.1299/jsmemag.109.1048_144.

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Auernheimer, Brent. "GSS, professional culture, geography, and software engineering." ACM SIGDOC Asterisk Journal of Computer Documentation 22, no. 2 (May 1998): 23–26. http://dx.doi.org/10.1145/291391.291393.

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Allori, Alexander C., Alexander M. Sailon, Elizabeth Clark, Cornelia Cretiu-Vasiliu, James Smay, John L. Ricci, and Stephen M. Warren. "Dynamic cell culture for vascularized bone engineering." Journal of the American College of Surgeons 207, no. 3 (September 2008): S51—S52. http://dx.doi.org/10.1016/j.jamcollsurg.2008.06.108.

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Raredon, Micha Sam Brickman, Kevin A. Rocco, Ciprian P. Gheorghe, Amogh Sivarapatna, Mahboobe Ghaedi, Jenna L. Balestrini, Thomas L. Raredon, Elizabeth A. Calle, and Laura E. Niklason. "Biomimetic Culture Reactor for Whole-Lung Engineering." BioResearch Open Access 5, no. 1 (May 2016): 72–83. http://dx.doi.org/10.1089/biores.2016.0006.

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KURODA, Shoichi. "Production Engineering Strength as Culture of Japan." Journal of the Japan Society for Precision Engineering 73, no. 1 (2007): 11–14. http://dx.doi.org/10.2493/jjspe.73.11.

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Greenberger, Joel S. "Combinatorial Cell Culture Techniques in Tissue Engineering." e-biomed: The Journal of Regenerative Medicine 1, no. 10 (October 24, 2000): 137–39. http://dx.doi.org/10.1089/152489000750009802.

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Bedingham, K. "Corporate culture change in the engineering environment." Engineering Management 14, no. 5 (October 1, 2004): 24–27. http://dx.doi.org/10.1049/em:20040505.

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Nagasaki, Y., M. Ichino, and K. Yoshimoto. "Biosurface Design for Patterned Cell Culture Engineering." Transactions of the Materials Research Society of Japan 33, no. 3 (2008): 755–58. http://dx.doi.org/10.14723/tmrsj.33.755.

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Robinson, J. Gregg, and Judith S. McIlwee. "Men, Women, and the Culture of Engineering." Sociological Quarterly 32, no. 3 (September 1, 1991): 403–21. http://dx.doi.org/10.1111/j.1533-8525.1991.tb00166.x.

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Inamdar, Niraj K., and Jeffrey T. Borenstein. "Microfluidic cell culture models for tissue engineering." Current Opinion in Biotechnology 22, no. 5 (October 2011): 681–89. http://dx.doi.org/10.1016/j.copbio.2011.05.512.

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Ferris, Tim. "Ethnic Culture and the Systems Engineering Process." INSIGHT 11, no. 1 (January 2008): 28–31. http://dx.doi.org/10.1002/inst.200811128.

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