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Journal articles on the topic 'Dual hybridization'

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Author: Grafiati

Published: 7 July 2024

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1

Wang, Joseph, Abdel-Nasser Kawde, Mustafa Musameh, and Gustavo Rivas. "Dual enzyme electrochemical coding for detecting DNA hybridization." Analyst 127, no. 10 (September 5, 2002): 1279–82. http://dx.doi.org/10.1039/b207424a.

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2

Troxell, Megan, Richard K. Sibley, Robert B. West, Gregory R. Bean, and Kimberly H. Allison. "HER2 Dual In Situ Hybridization: Correlations and Cautions." Archives of Pathology & Laboratory Medicine 144, no. 12 (February 26, 2020): 1525–34. http://dx.doi.org/10.5858/arpa.2019-0510-oa.

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Context.— Accurate HER2 testing in breast cancer is crucial for appropriate precision therapy. HER2 testing is most commonly accomplished by a combination of immunohistochemistry and in situ hybridization techniques, as gene amplification is closely tied to protein overexpression. During the last 5+ years, brightfield dual in situ hybridization (DISH) has replaced fluorescence methods (fluorescence in situ hybridization [FISH]) in some laboratories. Objective.— To analyze routine HER2 DISH performance in the field. Design.— We reviewed our experience with HER2 DISH performed at outside laboratories and referred for patient care. Results.— Of 273 identified retrospective DISH results, 55 had repeated FISH testing at our institution; 7 (13%) were discordant. Additional cases had technical flaws hampering appropriate scoring. In 23 cases (42%), HER2 DISH was performed without immunohistochemistry. Slide review of a prospective cohort of 42 consecutive DISH cases revealed 14 (33%) with technical or interpretative limitations potentially jeopardizing results. Commonly identified problems include lack of or weak signals in most tumor cells, and silver precipitate or red signals outside of nuclei, resulting in false-negative or false-positive interpretations, respectively. Further, 44% (24 of 55) of DISH reports lacked complete data, specifically average HER2 signals/cell. Conclusions.— While HER2 DISH can be an efficient and effective alternative to FISH, we illustrate pitfalls and reinforce that careful attention to slide quality and technical parameters are critically important. HER2 DISH cotesting with immunohistochemistry could help minimize false-negative or false-positive HER2 results.

3

Bonanni, Alessandra, Daniel Calvo, and Manuel del Valle. "Dual-Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy." Electroanalysis 20, no. 9 (May 2008): 941–48. http://dx.doi.org/10.1002/elan.200704184.

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4

Kosa, Csaba, Laszlo Kardos, Judit Kovacs, and Zoltan Szollosi. "Comparison of dual-color dual-hapten brightfield in situ hybridization (DDISH) and fluorescence in situ hybridization in breast cancer HER2 assessment." Pathology - Research and Practice 209, no. 3 (March 2013): 147–50. http://dx.doi.org/10.1016/j.prp.2013.01.006.

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5

Gao, Faye F., David J. Dabbs, Kristine L. Cooper, and Rohit Bhargava. "Bright-Field HER2 Dual In Situ Hybridization (DISH) Assay vs Fluorescence In Situ Hybridization (FISH)." American Journal of Clinical Pathology 141, no. 1 (January 1, 2014): 102–10. http://dx.doi.org/10.1309/ajcp6cxs8osrhxir.

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6

Jbari, Hatim, Mohamed Haidoury, Rachid Askour, and Badr Bououlid Idrissi. "Fuzzy Logic Controller for an EV’s Dual-Source Hybridization." E3S Web of Conferences 297 (2021): 01039. http://dx.doi.org/10.1051/e3sconf/202129701039.

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This paper presents an energy management system (EMS) based on fuzzy logic control (FLC) strategy combined with power filtering. This strategy is developed for an Electric Vehicle (EV) hybrid energy storage systems (HESS). The proposed control and energy management strategy (EMS) aims to ensure an efficient power split guaranteeing that battery and supercapacitors (SC) provide the continuous and transient-power, respectively, adopting a pure electric vehicle fully-active parallel topology. In order to develop the studied system model, the Energetic Macroscopic Representation (EMR) approach is adopted. Considering SC’s control criterion, and battery root mean square RMS current reducing, an evaluation of the proposed EMS and developed model was conducted using MATLAB/SIMULINK simulation under New European Driving Cycle (NEDC) and compared to the classical only battery storage configuration.

7

Gong, Shaokang, Binggang Xiao, Lihua Xiao, Shengjun Tong, Sanshui Xiao, and Xiumin Wang. "Hybridization-induced dual-band tunable graphene metamaterials for sensing." Optical Materials Express 9, no. 1 (December 4, 2018): 35. http://dx.doi.org/10.1364/ome.9.000035.

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8

Zhou, Dongrui, Wanqiong Qiao, Yuan Wan, and Zuhong Lu. "Microarray-based methylation analysis using dual-color fluorescence hybridization." Journal of Biochemical and Biophysical Methods 66, no. 1-3 (March 2006): 33–43. http://dx.doi.org/10.1016/j.jbbm.2005.11.004.

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9

Yang, Peng, Takanori Hirose, Tadashi Hasegawa, Kazuo Hizawa, and Toshiaki Sano. "Dual-colour fluorescencein situ hybridization analysis of synovial sarcoma." Journal of Pathology 184, no. 1 (January 1998): 7–13. http://dx.doi.org/10.1002/(sici)1096-9896(199801)184:1<7::aid-path978>3.0.co;2-x.

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10

Ren, Huizhu, Shupei Zhang, Yitian Huang, Yanjie Chen, Liang Lv, and Hong Dai. "Dual-readout proximity hybridization-regulated and photothermally amplified protein analysis based on MXene nanosheets." Chemical Communications 56, no. 87 (2020): 13413–16. http://dx.doi.org/10.1039/d0cc05148a.

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11

Shet, TanujaM, PoojaK Gajaria, Sonali Tambe, Trupti Pai, Asawari Patil, and SangeetaB Desai. "Dual-color dual-hapten in situ hybridization (D-DISH) – Comparison with fluorescence in situ hybridization (FISH) for HER2/neu testing in breast cancer." Indian Journal of Pathology and Microbiology 63, no. 2 (2020): 194. http://dx.doi.org/10.4103/ijpm.ijpm_861_19.

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12

Miao, Peng, Yuguo Tang, and Jian Yin. "MicroRNA detection based on analyte triggered nanoparticle localization on a tetrahedral DNA modified electrode followed by hybridization chain reaction dual amplification." Chemical Communications 51, no. 86 (2015): 15629–32. http://dx.doi.org/10.1039/c5cc05499k.

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13

Giannakopoulou, Erofili, Vasiliki Pardali, Efseveia Frakolaki, Vasileios Siozos, Vassilios Myrianthopoulos, Emmanuel Mikros, Martin C. Taylor, John M. Kelly, Niki Vassilaki, and Grigoris Zoidis. "Scaffold hybridization strategy towards potent hydroxamate-based inhibitors ofFlaviviridaeviruses andTrypanosomaspecies." MedChemComm 10, no. 6 (2019): 991–1006. http://dx.doi.org/10.1039/c9md00200f.

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14

do Couto Maia, Rodolfo, and Carlos Alberto Manssour Fraga. "Discovery of Dual Chemotherapy Drug Candidates Designed by Molecular Hybridization." Current Enzyme Inhibition 6, no. 4 (December 1, 2010): 171–82. http://dx.doi.org/10.2174/157340810794578515.

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15

Rantanen, Terhi, Marja-Leena Järvenpää, Johanna Vuojola, Riikka Arppe, Katri Kuningas, and Tero Soukka. "Upconverting phosphors in a dual-parameter LRET-based hybridization assay." Analyst 134, no. 8 (2009): 1713. http://dx.doi.org/10.1039/b901299k.

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16

Beraki, Elsa, and Torill Sauer. "Determination of HER-2 status on FNAC material from breast carcinomas using in situ hybridization with dual chromogen visualization with silver enhancement (dual SISH)." CytoJournal 7 (October 11, 2010): 21. http://dx.doi.org/10.4103/1742-6413.70968.

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During the last years, HER-2 status kits and protocols for chromogen visualization of hybridization signals have come on the market. The first generation using chromogen visualization used single color probes. The second generation, now emerging on the market, uses dual chromogen visualization. The aim of this study has been to test a new dual color chromogen kit (Ventana INFORM HER2 Dual Colour ISH Roche®) and compare the results with our in-house method(s). The material consisted primarily of cytological material from invasive breast carcinomas in 49 women. Dual SISH was done on all 49 cytological and histological specimens. The histological specimens were treated according to the manufacturer’s recommendations. The procedure was modified in several steps in order to adapt it to the cytological material. Hybridization failed in two cytological specimens. Dual SISH showed concordant results on cytological and histological material as to amplified/not amplified. The included cases had the same HER-2 expression in the invasive and the in situ components on histology. Four IDC showed HER-2 amplification (8.5%). Polysomy was found in two cases. All dual SISH results except for one concurred with the results of the in-house method(s) (1/47=2.1%). The dual SISH is suitable for cytological examination of HER-2 status. The protocol must be optimized for cytological material.

17

Mahajan, Shilpi, Hui Zhao, Kristina Kovacina, Eric Lachacz, Sany Hoxha, Josolyn Chan, and Meina Liang. "High-sensitivity quantification of antisense oligonucleotides for pharmacokinetic characterization." Bioanalysis 14, no. 9 (May 2022): 603–13. http://dx.doi.org/10.4155/bio-2022-0035.

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Aim: Antisense oligonucleotides (ASOs) are a fast-growing drug modality. Pharmacokinetic characterization and accurate quantification of ASOs is critical for drug development. LC–MS and hybridization immunoassays are common methods to quantify ASOs but may lack sensitivity. In this study we aimed to develop an ASO quantification method with improved sensitivity. Methods: We developed a branched DNA approach for ASO quantification and compared it with hybridization immunoassays. Results: The branched DNA assay showed significantly improved sensitivity, with LLOQ 31.25 pg/ml in plasma, 6.4-and 16-fold higher than dual-probe hybridization electrochemiluminescence and single-probe hybridization ELISA, respectively, with adequate precision, accuracy, selectivity and specificity and acceptable matrix interference. Conclusion: Branched DNA for ASO quantification has significantly higher sensitivity and lower hemolysis interference.

18

Gavvala, Krishna, Nicolas P. F. Barthes, Dominique Bonhomme, Anne Sophie Dabert-Gay, Delphine Debayle, Benoît Y. Michel, Alain Burger, and Yves Mély. "A turn-on dual emissive nucleobase sensitive to mismatches and duplex conformational changes." RSC Advances 6, no. 90 (2016): 87142–46. http://dx.doi.org/10.1039/c6ra19061h.

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Herein, we demonstrate the on–off dual emissive behaviour of a fluorescent nucleoside sensitive towards DNA hybridization and conformational changes as well as detection of single nucleotide polymorphisms.

19

Zhou, Ping, Shaohua Gong, Bo Liu, Mingwan Shi, Fei Lu, Na Li, and Bo Tang. "A hybridization-based dual-colorimetric kit for circulating cancer miRNA detection." Chemical Communications 57, no. 49 (2021): 6058–61. http://dx.doi.org/10.1039/d1cc01607e.

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A dual-colorimetric miRNA detection kit that can simultaneously detect two miRNAs with high sensitivity and selectivity is developed, which is able to achieve the rapid detection of lung cancer using clinical serum samples.

20

Ryu, Jaehoon, Eunwoo Lee, Kisu Lee, and Jyongsik Jang. "A graphene quantum dots based fluorescent sensor for anthrax biomarker detection and its size dependence." Journal of Materials Chemistry B 3, no. 24 (2015): 4865–70. http://dx.doi.org/10.1039/c5tb00585j.

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Graphene quantum dots (GQDs) with two different diameters were modified via hybridization with a EuIII–macromolecule complex, and their application as dual emission fluorescent sensors for detection of Bacillus anthracis spores was investigated.

21

Hwang, Cheng-Cheng, Mariann Pintye, Liang-Che Chang, Huang-Yang Chen, Kun-Yan Yeh, Hui-Ping Chein, Nin Lee, and Jim-Ray Chen. "Dual-colour chromogenic in-situ hybridization is a potential alternative to fluorescence in-situ hybridization in HER2 testing." Histopathology 59, no. 5 (November 2011): 984–92. http://dx.doi.org/10.1111/j.1365-2559.2011.04037.x.

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22

Dupin, Nicolas, and El-Ghazali Talbi. "Machine Learning-Guided Dual Heuristics and New Lower Bounds for the Refueling and Maintenance Planning Problem of Nuclear Power Plants." Algorithms 13, no. 8 (July 30, 2020): 185. http://dx.doi.org/10.3390/a13080185.

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This paper studies the hybridization of Mixed Integer Programming (MIP) with dual heuristics and machine learning techniques, to provide dual bounds for a large scale optimization problem from an industrial application. The case study is the EURO/ROADEF Challenge 2010, to optimize the refueling and maintenance planning of nuclear power plants. Several MIP relaxations are presented to provide dual bounds computing smaller MIPs than the original problem. It is proven how to get dual bounds with scenario decomposition in the different 2-stage programming MILP formulations, with a selection of scenario guided by machine learning techniques. Several sets of dual bounds are computable, improving significantly the former best dual bounds of the literature and justifying the quality of the best primal solution known.

23

Tremblay, GA, G. Khalafaghian, J. Legault, P. Nielsen, and AJ Bartlett. "Dual ligation hybridization assay for the specific determination of oligonucleotide therapeutics." Bioanalysis 3, no. 5 (March 2011): 499–508. http://dx.doi.org/10.4155/bio.11.18.

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24

Wang, Yihan, Huan Feng, Ke Huang, Jinfeng Quan, Fangfang Yu, Xiaohui Liu, Hui Jiang, and Xuemei Wang. "Target-triggered hybridization chain reaction for ultrasensitive dual-signal miRNA detection." Biosensors and Bioelectronics 215 (November 2022): 114572. http://dx.doi.org/10.1016/j.bios.2022.114572.

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25

Smith, M. L. "Visualization of FISH Probes by Dual-Color Chromogenic In Situ Hybridization." Yearbook of Pathology and Laboratory Medicine 2011 (January 2011): 229–30. http://dx.doi.org/10.1016/j.ypat.2010.10.021.

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26

Sadana, A., and T. Vo-Dinh. "Single- and Dual-Fractal Analysis of Hybridization Binding Kinetics: Biosensor Applications." Biotechnology Progress 14, no. 5 (October 2, 1998): 782–90. http://dx.doi.org/10.1021/bp980081r.

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27

Qun, Liu, and Zhu Guijin. "Detection of embryo sex chromosome by dual color fluorescentin-situ hybridization." Journal of Huazhong University of Science and Technology [Medical Sciences] 23, no. 2 (June 2003): 187–89. http://dx.doi.org/10.1007/bf02859953.

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28

Hoff, Kirsten, Jan T. Jørgensen, Sven Müller, Else Røngaard, Ole Rasmussen, and Andreas Schønau. "Visualization of FISH Probes by Dual-Color Chromogenic In Situ Hybridization." American Journal of Clinical Pathology 133, no. 2 (February 2010): 205–11. http://dx.doi.org/10.1309/ajcp12mhrtfzjpkw.

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29

Lee, Dakeun, Jinwon Seo, YoungLyun Oh, Jinman Kim, and YoungHyeh Ko. "Analysis of follicular lymphoma by dual-color fluorescence in situ hybridization." Virchows Archiv 452, no. 1 (November 27, 2007): 75–81. http://dx.doi.org/10.1007/s00428-007-0531-3.

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30

Babic, Andrea, Isabell R. Loftin, Stacey Stanislaw, Maria Wang, Rachel Miller, Stephanie M. Warren, Wenjun Zhang, et al. "The impact of pre-analytical processing on staining quality for H&E, dual hapten, dual color in situ hybridization and fluorescent in situ hybridization assays." Methods 52, no. 4 (December 2010): 287–300. http://dx.doi.org/10.1016/j.ymeth.2010.08.012.

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31

Xing, Chao, Ziyi Chen, Cheng Zhang, Jun Wang, and Chunhua Lu. "Target-directed enzyme-free dual-amplification DNA circuit for rapid signal amplification." Journal of Materials Chemistry B 8, no. 47 (2020): 10770–75. http://dx.doi.org/10.1039/d0tb02114h.

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An enzyme-free, single-step and rapid signal amplification DNA circuit was developed by integrating target-directed entropy-driven catalysis and hybridization chain reaction for fluorescence analysis of nucleic acids and small molecules.

32

Hine, Anna V., Xianfeng Chen, Marcus D. Hughes, Kaiming Zhou, Edward Davies, Kate Sugden, Ian Bennion, and Lin Zhang. "Optical fibre-based detection of DNA hybridization." Biochemical Society Transactions 37, no. 2 (March 20, 2009): 445–49. http://dx.doi.org/10.1042/bst0370445.

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A dual-peak LPFG (long-period fibre grating), inscribed in an optical fibre, has been employed to sense DNA hybridization in real time, over a 1 h period. One strand of the DNA was immobilized on the fibre, while the other was free in solution. After hybridization, the fibre was stripped and repeated detection of hybridization was achieved, so demonstrating reusability of the device. Neither strand of DNA was fluorescently or otherwise labelled. The present paper will provide an overview of our early-stage experimental data and methodology, examine the potential of fibre gratings for use as biosensors to monitor both nucleic acid and other biomolecular interactions and then give a summary of the theory and fabrication of fibre gratings from a biological standpoint. Finally, the potential of improving signal strength and possible future directions of fibre grating biosensors will be addressed.

33

Gomgnimbou, M. K., E. Abadia, J. Zhang, G. Refregier, S. Panaiotov, E. Bachiyska, and C. Sola. ""Spoligoriftyping," a Dual-Priming-Oligonucleotide-Based Direct-Hybridization Assay for Tuberculosis Control with a Multianalyte Microbead-Based Hybridization System." Journal of Clinical Microbiology 50, no. 10 (July 18, 2012): 3172–79. http://dx.doi.org/10.1128/jcm.00976-12.

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34

Horii, Rie, Masaaki Matsuura, Takuji Iwase, Yoshinori Ito, and Futoshi Akiyama. "Comparison of dual-color in-situ hybridization and fluorescence in-situ hybridization in HER2 gene amplification in breast cancer." Breast Cancer 21, no. 5 (January 12, 2013): 598–604. http://dx.doi.org/10.1007/s12282-012-0436-0.

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35

Yuce, Meral, Hasan Kurt, and Hikmet Budak. "Characterization of a dual biotin tag for improved single stranded DNA production." Anal. Methods 6, no. 2 (2014): 548–57. http://dx.doi.org/10.1039/c3ay41899e.

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Generation of single-stranded DNA plays a key role in many biotechnology applications including production of aptamers, single strand conformation polymorphism, nuclease S1 mapping, pyrosequencing, genosensors, probe preparation and labelling, subtractive hybridization as well as nucleic acid sensing and microarrays.

36

Quan, Ke, Jing Li, Jiaoli Wang, Nuli Xie, Qiaomei Wei, Jinlu Tang, Xiaohai Yang, Kemin Wang, and Jin Huang. "Dual-microRNA-controlled double-amplified cascaded logic DNA circuits for accurate discrimination of cell subtypes." Chemical Science 10, no. 5 (2019): 1442–49. http://dx.doi.org/10.1039/c8sc04887h.

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We have designed dual-microRNA-controlled cascaded logic DNA circuits for cancer cell subtype identification. The basic idea is to improve sensitivity by cascading DNAzyme and hybridization chain reaction (HCR), and improve accuracy by simultaneous detection of miR-122 and miR-21.

37

Yang, Liu, and Yanfu Li. "Dual signal-amplification electrochemical detection of DNA sequence based on molybdenum selenide nanorod and hybridization chain reaction." Analytical Methods 8, no. 26 (2016): 5234–41. http://dx.doi.org/10.1039/c6ay01425a.

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A new electrochemical biosensor based on the hybridization chain reaction and layered molybdenum selenide (MoSe₂) stacked nanorod for dual-signal amplification is developed for the highly sensitive detection of the DNA sequences of Human Immunodeficiency Virus type 1 (HIV-1).

38

Lin, Qing, Shijun Cai, Bing Zhou, Kemin Wang, Lixin Jian, and Jin Huang. "Dual-MicroRNA-regulation of singlet oxygen generation by a DNA-tetrahedron-based molecular logic device." Chemical Communications 57, no. 32 (2021): 3873–76. http://dx.doi.org/10.1039/d1cc00818h.

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A DNA-tetrahedron-based ‘‘AND’’ logic gate is utilized as a molecular device that recognizes dual-miRNA inputs through strand hybridization to activate computation cascades that produce ¹O₂ in live cells, resulting in the death of target cells.

39

Mu, Haiwei, Jianxin Wang, Qiang Liu, Wei Liu, Xianli Li, Jingwei Lv, Chao Liu, Famei Wang, Tao Sun, and Paul K. Chu. "Localized Surface Plasmon Resonance Properties of Concentric Dual-Ring Nanodisk." Nano 14, no. 06 (June 2019): 1950071. http://dx.doi.org/10.1142/s1793292019500711.

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The extinction spectral properties based on localized surface plasmon resonance (LSPR) of the concentric dual-ring nanodisk (CDRN) structure are investigated by discrete dipole approximation (DDA) and plasmon hybridization theory. The CDRN nanostructure shows flexible tunable multipole resonances in the near-infrared regime and the coupled resonance wavelengths depend on the structural parameters of the nanostructure, which has great potential in multichannel LSPR-based bio-sensing applications.

40

Chen, Qiaoshu, Chunying Li, Xiaohai Yang, Jin Huang, Songyang Liu, Wei Liu, Jianbo Liu, and Kemin Wang. "Self-assembled DNA nanowires as quantitative dual-drug nanocarriers for antitumor chemophotodynamic combination therapy." Journal of Materials Chemistry B 5, no. 36 (2017): 7529–37. http://dx.doi.org/10.1039/c7tb01590a.

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Self-assembled DNA nanowires were fabricated through a supersandwich hybridization reaction and co-loaded with a photosensitizer chlorin e6 (Ce6) and a chemotherapeutic drug doxorubicin (DOX) for antitumor chemophotodynamic combination therapy.

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Sedlmayr, Nicholas, and Alex Levchenko. "Hybridization mechanism of the dual proximity effect in superconductor–topological insulator interfaces." Solid State Communications 327 (March 2021): 114221. http://dx.doi.org/10.1016/j.ssc.2021.114221.

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Volkan Demir, Hilmi, Sedat Nizamoglu, Tuncay Ozel, Evren Mutlugun, Ilkem Ozge Huyal, Emre Sari, Elisabeth Holder, and Nan Tian. "White light generation tuned by dual hybridization of nanocrystals and conjugated polymers." New Journal of Physics 9, no. 10 (October 5, 2007): 362. http://dx.doi.org/10.1088/1367-2630/9/10/362.

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Li, Xinyun, Sung-Hock Chew, Wen-Yee Chay, Soo-Kim Lim-Tan, and Liang-Kee Goh. "Optimizing Ventana chromogenic dual in-situ hybridization for mucinous epithelial ovarian cancer." BMC Research Notes 6, no. 1 (2013): 562. http://dx.doi.org/10.1186/1756-0500-6-562.

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44

Zaidi, Aliya U., Hideki Enomoto, Jeffrey Milbrandt, and Kevin A. Roth. "Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection with Tyramide Signal Amplification." Journal of Histochemistry & Cytochemistry 48, no. 10 (October 2000): 1369–75. http://dx.doi.org/10.1177/002215540004801007.

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45

Lucas-Garrote, Beatriz, Sergi Morais, and Ángel Maquieira. "Dual signal amplification for highly sensitive hybridization microassays on chemically activated surfaces." Sensors and Actuators B: Chemical 246 (July 2017): 1108–15. http://dx.doi.org/10.1016/j.snb.2016.10.109.

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46

Li, Song, Hongna Liu, Zhifei Wang, Peng Hou, Yafei Guo, Quanguo He, and Nongyue He. "Magnetic-particles-based high-throughput genotyping method with dual-color fluorescence hybridization." Analytical Biochemistry 359, no. 2 (December 2006): 277–79. http://dx.doi.org/10.1016/j.ab.2006.07.040.

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47

Chen, Wei-Jen, Shih-Chieh Wang, Po-Cheng Chen, Tsorng-Wen Chen, and Kan-Nan Chen. "Hybridization of aqueous PU/epoxy resin via a dual self-curing process." Journal of Applied Polymer Science 110, no. 1 (October 5, 2008): 147–55. http://dx.doi.org/10.1002/app.26049.

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48

Lago, Luiza Grazziotin, Bukky Dada, Hua Yang, and Deborah Marshall. "PP06 HER2 Evaluation By CISH And SISH In Breast Cancer: A Meta-Analysis." International Journal of Technology Assessment in Health Care 34, S1 (2018): 68–69. http://dx.doi.org/10.1017/s0266462318001836.

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Introduction:Molecular techniques play a critical role in identifying breast cancer patients with overexpressed human epidermal growth factor receptor-2 (HER2). New bright field techniques such as chromogenic in-situ hybridization (CISH) and silver in-situ hybridization (SISH) have emerged to overcome some of the challenges associated with the reference standard, fluorescence in-situ hybridization (FISH). We conducted a literature review and synthesis to characterize the accuracy of HER2 tests, and inform decisions about test selection.Methods:We searched MEDLINE and EMBASE databases using these eligibility criteria: studies evaluating invasive breast cancer samples which examined agreement between CISH or SISH, and FISH, and reported sensitivity, specificity, or concordance. We performed a bivariate meta-analysis of sensitivity and specificity using a generalized linear mixed model in Stata. We used likelihood ratio tests from meta-regression to compare accuracy between HER2 tests.Results:The search identified 4,475 articles, of which thirty-one were included. A total of thirteen studies (43%) evaluated dual-color SISH, twelve single-color CISH, and six dual-color CISH. The summary estimates for sensitivity and specificity were, respectively, 0.97 (95%CI 0.83–0.99) and 0.99 (95%CI 0.96–1.00) for single-color CISH, 0.98 (95%CI 0.92–0.99) and 0.98 (95%CI 0.91–0.99) for dual-color CISH; 0.92 (95%CI 0.86–0.95), and 0.96 (95%CI 0.91–0.98) for SISH. Significantly higher specificity was reported for single-color CISH than SISH (chi-square 4.12; p = 0.04), while dual-CISH had higher sensitivity than SISH (chi-square: 4.63; p = 0.03). These differences were not maintained when studies with cohorts enriched with equivocal samples were excluded.Conclusions:The agreement between new bright field tests (SISH and CISH) and FISH is high (>92 percent). Indirect comparison of HER2 tests indicated that overall CISH performance exceeds that of SISH. However, low agreement between SISH and FISH in equivocal cases affects these comparative estimates. The pooled estimates from this meta-analysis can help inform future HER2 test selection decisions.

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Zhang, Gloria, Christopher P. Lanigan, John R. Goldblum, Raymond R. Tubbs, and Erinn Downs-Kelly. "Automated Bright-Field Dual-Color In Situ Hybridization for MDM2: Interobserver Reproducibility and Correlation With Fluorescence In Situ Hybridization in a Series of Soft Tissue Consults." Archives of Pathology & Laboratory Medicine 140, no. 10 (October 1, 2016): 1111–15. http://dx.doi.org/10.5858/arpa.2015-0249-oa.

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Abstract:

Context.—Atypical lipomatous tumors/well-differentiated liposarcomas contain alterations in the 12q13-15 region resulting in amplification of MDM2 and nearby genes. Identifying MDM2 amplification is a useful ancillary test, as the histologic mimics of atypical lipomatous tumors/well-differentiated liposarcomas have consistently shown a lack of MDM2 amplification. Objective.—To assess the interobserver reproducibility of a bright-field assay for MDM2 amplification (dual-color, dual-hapten in situ hybridization [DDISH]) among reviewers with varying degrees of experience with the assay and to assess the concordance of MDM2 DDISH with MDM2 fluorescence in situ hybridization (FISH). Design.—In total, 102 cases were assessed in parallel for MDM2 by FISH and DDISH. MDM2 amplification was defined as an MDM2 to chromosome 12 ratio of 2.0 or greater, whereas an MDM2 to chromosome 12 ratio of less than 2 was nonamplified. Fluorescence in situ hybridization was scored in the routine clinical laboratory and DDISH was evaluated by 3 different pathologists blinded to the final diagnosis and FISH results. Results.—Fluorescence in situ hybridization categorized 27 cases (26%) as MDM2 amplified and 75 cases (74%) as nonamplified; the consensus DDISH diagnosis was 98% concordant with FISH. Agreement between MDM2 DDISH by each reviewer and MDM2 FISH was highly concordant (99%, 98%, and 98%, respectively, for reviewers 1, 2 and 3). The κ agreement of the 3 reviewers scoring DDISH was excellent (κ = 0.949, 0.95, and 0.95, respectively, for reviewers 1, 2, and 3). Conclusions.—This study highlights excellent concordance between DDISH and FISH in MDM2 copy number assessment. Moreover, excellent interobserver reproducibility of the DDISH assay was found among reviewers with varying levels of experience evaluating bright-field assays.

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Yu, Xiang, Yi Yong Yang, and Cheng Biao Wang. "Effect of Pulsed Substrate Bias on Evolution of Surface Morphology and sp³ Hybridization Degree of Ag-DLC Films in a Mid-Frequency Dual-Magnetron Sputtering." Advanced Materials Research 105-106 (April 2010): 444–47. http://dx.doi.org/10.4028/www.scientific.net/amr.105-106.444.

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In this work, we have investigated the influence of the pulsed substrate bias voltage on the evolution of the surface morphology and sp3 hybridization degree of Ag containing diamond-like carbon (Ag-DLC) films deposited by using a mid-frequency dual-magnetron sputtering system. The unipole substrate bias voltage at 0 V, -60 V, -100 V and -150 V, respectively, was employed on AISI 440 substrate with the duty ratio uniformly set at 70%. The surface morphology was observed by AFM and the hybridization degree of the DLC films was performed respectively using Raman Spectroscopy and Rockwell C indenter. In these samples, the surface morphology and sp3 hybridization degree of Ag-DLC films show a certain dependence on the pulsed substrate bias applied. The evolution tendency of the surface morphology is found different with that of the sp3 hybridization degree of the DLC films under action of the pulsed substrate bias. These phenomena imply that the energetic particles may induce a balance between recombination of the particles and change of the internal stress in the DLC films.