Relevant bibliographies by topics / Dual hybridization

Academic literature on the topic 'Dual hybridization'

Author: Grafiati
Published: 7 July 2024

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Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Journal articles on the topic "Dual hybridization":

1

Wang, Joseph, Abdel-Nasser Kawde, Mustafa Musameh, and Gustavo Rivas. "Dual enzyme electrochemical coding for detecting DNA hybridization." Analyst 127, no. 10 (September 5, 2002): 1279–82. http://dx.doi.org/10.1039/b207424a.

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Troxell, Megan, Richard K. Sibley, Robert B. West, Gregory R. Bean, and Kimberly H. Allison. "HER2 Dual In Situ Hybridization: Correlations and Cautions." Archives of Pathology & Laboratory Medicine 144, no. 12 (February 26, 2020): 1525–34. http://dx.doi.org/10.5858/arpa.2019-0510-oa.

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Context.— Accurate HER2 testing in breast cancer is crucial for appropriate precision therapy. HER2 testing is most commonly accomplished by a combination of immunohistochemistry and in situ hybridization techniques, as gene amplification is closely tied to protein overexpression. During the last 5+ years, brightfield dual in situ hybridization (DISH) has replaced fluorescence methods (fluorescence in situ hybridization [FISH]) in some laboratories. Objective.— To analyze routine HER2 DISH performance in the field. Design.— We reviewed our experience with HER2 DISH performed at outside laboratories and referred for patient care. Results.— Of 273 identified retrospective DISH results, 55 had repeated FISH testing at our institution; 7 (13%) were discordant. Additional cases had technical flaws hampering appropriate scoring. In 23 cases (42%), HER2 DISH was performed without immunohistochemistry. Slide review of a prospective cohort of 42 consecutive DISH cases revealed 14 (33%) with technical or interpretative limitations potentially jeopardizing results. Commonly identified problems include lack of or weak signals in most tumor cells, and silver precipitate or red signals outside of nuclei, resulting in false-negative or false-positive interpretations, respectively. Further, 44% (24 of 55) of DISH reports lacked complete data, specifically average HER2 signals/cell. Conclusions.— While HER2 DISH can be an efficient and effective alternative to FISH, we illustrate pitfalls and reinforce that careful attention to slide quality and technical parameters are critically important. HER2 DISH cotesting with immunohistochemistry could help minimize false-negative or false-positive HER2 results.
3

Bonanni, Alessandra, Daniel Calvo, and Manuel del Valle. "Dual-Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy." Electroanalysis 20, no. 9 (May 2008): 941–48. http://dx.doi.org/10.1002/elan.200704184.

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Kosa, Csaba, Laszlo Kardos, Judit Kovacs, and Zoltan Szollosi. "Comparison of dual-color dual-hapten brightfield in situ hybridization (DDISH) and fluorescence in situ hybridization in breast cancer HER2 assessment." Pathology - Research and Practice 209, no. 3 (March 2013): 147–50. http://dx.doi.org/10.1016/j.prp.2013.01.006.

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Gao, Faye F., David J. Dabbs, Kristine L. Cooper, and Rohit Bhargava. "Bright-Field HER2 Dual In Situ Hybridization (DISH) Assay vs Fluorescence In Situ Hybridization (FISH)." American Journal of Clinical Pathology 141, no. 1 (January 1, 2014): 102–10. http://dx.doi.org/10.1309/ajcp6cxs8osrhxir.

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Jbari, Hatim, Mohamed Haidoury, Rachid Askour, and Badr Bououlid Idrissi. "Fuzzy Logic Controller for an EV’s Dual-Source Hybridization." E3S Web of Conferences 297 (2021): 01039. http://dx.doi.org/10.1051/e3sconf/202129701039.

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This paper presents an energy management system (EMS) based on fuzzy logic control (FLC) strategy combined with power filtering. This strategy is developed for an Electric Vehicle (EV) hybrid energy storage systems (HESS). The proposed control and energy management strategy (EMS) aims to ensure an efficient power split guaranteeing that battery and supercapacitors (SC) provide the continuous and transient-power, respectively, adopting a pure electric vehicle fully-active parallel topology. In order to develop the studied system model, the Energetic Macroscopic Representation (EMR) approach is adopted. Considering SC’s control criterion, and battery root mean square RMS current reducing, an evaluation of the proposed EMS and developed model was conducted using MATLAB/SIMULINK simulation under New European Driving Cycle (NEDC) and compared to the classical only battery storage configuration.
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Gong, Shaokang, Binggang Xiao, Lihua Xiao, Shengjun Tong, Sanshui Xiao, and Xiumin Wang. "Hybridization-induced dual-band tunable graphene metamaterials for sensing." Optical Materials Express 9, no. 1 (December 4, 2018): 35. http://dx.doi.org/10.1364/ome.9.000035.

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Zhou, Dongrui, Wanqiong Qiao, Yuan Wan, and Zuhong Lu. "Microarray-based methylation analysis using dual-color fluorescence hybridization." Journal of Biochemical and Biophysical Methods 66, no. 1-3 (March 2006): 33–43. http://dx.doi.org/10.1016/j.jbbm.2005.11.004.

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Yang, Peng, Takanori Hirose, Tadashi Hasegawa, Kazuo Hizawa, and Toshiaki Sano. "Dual-colour fluorescencein situ hybridization analysis of synovial sarcoma." Journal of Pathology 184, no. 1 (January 1998): 7–13. http://dx.doi.org/10.1002/(sici)1096-9896(199801)184:1<7::aid-path978>3.0.co;2-x.

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Ren, Huizhu, Shupei Zhang, Yitian Huang, Yanjie Chen, Liang Lv, and Hong Dai. "Dual-readout proximity hybridization-regulated and photothermally amplified protein analysis based on MXene nanosheets." Chemical Communications 56, no. 87 (2020): 13413–16. http://dx.doi.org/10.1039/d0cc05148a.

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You might also be interested in the extended bibliographies on the topic 'Dual hybridization' for particular source types:
Journal articles

Dissertations / Theses on the topic "Dual hybridization":

1

Kondo, Masahiko. "Diverse p53 gene aberration in hepatocellular carcinoma detected by dual-color fluorescence in situ hybridization." Kyoto University, 2004. http://hdl.handle.net/2433/147542.

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Thomas, Komba. "Novel dual-acting nitroxide-based hybrid antioxidants." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/97691/9/Komba_Thomas_Thesis.pdf.

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This project employed a rational drug design and development approached to make hybrid dual-acting antioxidants as potential medications for managing chronic inflammation and oxidative stress-related diseases such as arthritis and Parkinson disease. The approach (pharmacophore hybridization strategy) combined various nitroxide compounds with known drugs - mainly the NSAID and sympathomimetic stimulants class of drugs. The hybrid compounds developed in this project displayed promising therapeutic actions as antioxidant, anti-inflammatory and anticancer agents.
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Paulus, Justine. "Le contrôle de gestion comme vecteur de transformation des organisations publiques confrontées à une complexité institutionnelle : le cas d’un Office Public de l’Habitat (OPH)." Electronic Thesis or Diss., Bourgogne Franche-Comté, 2023. http://www.theses.fr/2023UBFCG010.

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Notre thèse vise à répondre à la problématique : quel est le rôle du contrôle de gestion dans les Offices Publics de l’Habitat (OPH) confrontés à la complexité institutionnelle ? En étudiant ces organisations sujettes au pluralisme institutionnel (Greenwood et al., 2011), nous cherchons à enrichir la littérature (Château Terrisse, 2012 ; Boitier et Rivière, 2016 ; Renaud, 2017 ; Amans et al., 2020…) sur le rôle actif des dispositifs de contrôle de gestion dans l’hybridation de logiques potentiellement incompatibles. L’ancrage théorique de la thèse se situe dans le courant des microfondations de la théorie néo-institutionnelle (Thornton et Ocasio, 1999 ; Thornton et al., 2012).Notre démarche méthodologique est structurée en deux temps. Nous réalisons d’abord une étude qualitative sur Gesthome, un OPH en pleine mutation confronté à quatre logiques institutionnelles (bureaucratique, politique, de marché et professionnelle). Cette étude qualitative mobilise plusieurs méthodes de recueil et d’analyse des données : 16 entretiens semi-directifs auprès des parties prenantes internes et externes de cette organisation, l’analyse de 26 documents et des archives de 713 pages ainsi qu’une observation participante durant 3 ans (3 carnets de notes de 120 pages chacun). Ce travail de terrain minutieux est réalisé dans le cadre d’une Convention Industrielle de Formation par la Recherche (CIFRE).Pour l’analyse de contenu, nous utilisons le logiciel d’analyse qualitative N’Vivo. Puis, en complément de l’étude qualitative, une étude quantitative est menée auprès de 32 OPH sur le territoire français afin de vérifier la transférabilité des résultats obtenus au sein de Gesthome.Nous montrons que les dispositifs de contrôle de gestion permettent la coexistence des logiques, favorisent l’hybridation duale, voire « triale » des logiques en servant de base à des compromis. Il ressort de notre étude au sein de Gesthome et de l’étude quantitative quatre compromis (Amans et al., 2020) conduisant au polymorphisme organisationnel (Renaud, 2017) : un compromis des logiques politique et de marché, un compromis des logiques politique et professionnelle, un compromis des logiques de marché et professionnelle et enfin un compromis entre les trois logiques. En outre, nos travaux apportent un éclairage sur le pilotage des OPHLM encore balbutiant dans l'état de l'art
Our thesis aims to answer the question: What is the role of management control in Public Housing Offices (OPH) in the face of institutional complexity? By studying these organizations subject to institutional pluralism (Greenwood et al., 2011), we seek to enrich the literature (Château Terrisse, 2012 ; Boitier and Rivière, 2016; Renaud, 2017 ; Amans et al., 2020…) on the role active management control devices in the hybridization of potentially incompatible logics. The theoretical grounding of the thesis is in the current of micro-foundations of neo-institutional theory (Thornton and Ocasio, 1999; Thornton et al., 2012).Our methodological approach is structured in two stages. We first carry out a qualitative study on Gesthome, a rapidly changing OPH, confronted with four institutional logics (bureaucratic, political, market and professional). This qualitative study mobilizes several methods of data collection and analysis: 16 semi-structured interviews with internal and external stakeholders of this organization, the analysis of 26 documents and archives of 713 pages as well as participant observation during 3 years (3 notebooks of 120 pages each). This meticulous fieldwork is carried out within the framework of an Industrial Research Training Agreement (CIFRE).For content analysis, we use the qualitative analysis software N'Vivo. Then, in addition to the qualitative study, a quantitative study is conducted with 32 DPOs on French territory in order to verify the transferability of the results obtained within Gesthome.We show that the management control devices allow the coexistence of logics, promote the dual hybridization or even “triale” of logics, by serving as a basis for compromises. Our study within Gesthome and the quantitative study show four compromises (Amans et al., 2020) leading to organizational polymorphism (Renaud, 2017): a compromise of political and market logics, a compromise of political and professional, a compromise between market and professional logics and finally a compromise between the three logics. In addition, our work sheds light on the piloting of OPH still in their infancy in the state of the art
4

Smitz, Olsson Nikita. "Resande människor – Resande föremål : En diskussion kring Anders Sparrmans Oceaniensamling härrörande från hans deltagande vid James Cooks andra världsomsegling åren 1772-1775." Thesis, Uppsala universitet, Institutionen för arkeologi och antik historia, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-296525.

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In this thesis the Oceania collection of Anders Sparrman is examined and discussed. Anders Sparrman, who was a disciple of Carl von Linnaeus, participated on James Cooks second voyage around the world. During this voyage, Sparrman assembled the collection that today is stored at the museum of Ethnography in Stockholm. The collection is complemented by Sparrman’s own journal from his travels, providing his perspective on the items he collected and the islands he visited. The items in the collection are analyzed through the aspects of hybridization, colonial herit-age and repatriation together with how collections of this type, with a dual cultural heritage, can be exhibited in a way so that both a national and international audience can take part of it. A rel-evant question is also what further research that can be done on collections of this type. With this thesis I argue that a digital exhibition would be the best way to make collections of this kind available on a national and international level. Also research projects over country- and institutional- borders on these types of items would extend the knowledge about them and elabo-rate the whole history around these objects that both have a cultural value in Sweden and in the South Pacific.
5

Calegari, Eleonir João. "Supercondutividade em um modelo de hubbard d− p, em duas dimensões." Universidade Federal de Santa Maria, 2006. http://repositorio.ufsm.br/handle/1/3901.

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In the present work the Roth s two-pole approximation (Phys. Rev. 184, 451 (1969)) has been used to investigate the role of d− p hybridization in the normal and superconducting states of an extended d− p Hubbard model. Superconductivity with singlet dx2−y2 -wave pairing is treated by following Beenen and Edwards formalism (Phys. Rev. B 52, 13636 (1995)). In the first part of this work, the effects of the hybridization on the superconductivity, in the hole-doped regime, have been studied treating Roth s band shift within two different approximations. In the first one, the band shift has been calculated in the limit U →¥ (U is the Coulomb interaction), with zero temperature and without consider the superconducting effects. These regards, are restrict to the band shift. In the other parts of the problem, U, the temperature and the superconducting effects have been considered finites. In the second approximation, the Coulomb interaction, the temperature and the superconductivity have been considered in the calculation of some relevant correlation functions present in the Roth s band shift. The obtained results show that the hybridization acts in the sense of to suppresses the superconductivity. Also, it has been verified that the first approximation overstimates the effects of the hybridization on the superconductivity. In the second part of these work, hoppings to second-nearest-neighbors have been included in the model with the purpose of reproduces adequately the asymetries (mainly those related with the Fermi surface, band structure and phase diagram) between the hole- and electron-doped systems. Particularly, it is shown that the crossover from hole-like to electronlike Fermi surface is deeply affected by the d − p hybridization in the hole doping case. It has been verified that the effect of the hybridization is most pronounced around the saddle-points, where the superconducting gap is maximum in the particular case of dx2−y2 -wave symmetry. As a consequence, the critical temperature Tc is directly affected by the hibridization. Moreover, the obtained results suggest that in the hole doped regime, the hybridization may act on the transport properties of the system due to the sign changes of the Hall coefficient when the crossover of the Fermi surface occurs. In the electron doped case, the crossover in the Fermi surface is not verified. Nevertheless, as the hybridization suppresses the density of states near the Fermi level, the superconductivity is affected. The topology of the Fermi surface in the hole and electron doping regime agree with recent experimental ARPES results for La2−xSrxCuO4 (hole doping) and Nd2−xCexCuO4 (electron doping).
Neste trabalho, foi usada a aproximação de dois-pólos proposta por L. Roth (Phys. Rev. 184, 451 (1969)), para investigar os efeitos da hibridização no estado normal e no estado supercondutor de um modelo de Hubbard d − p. Para tratar supercondutividade com simetria dx2−y2 , usou-se o procedimento de fatorização proposto por Beenen e Edwards (Phys. Rev. B 52, 13636 (1995)). Na primeira parte do trabalho, os efeitos da hibridização sobre a upercondutividade, foram investigados considerando-se duas aproximações diferentes para calcular o deslocamento de banda. O deslocamento de banda surge quando tratamos as equações de movimento das funções de Green através do método de L. Roth. Na primeira aproximação o deslocamento foi calculado para U →¥ (U ´e a interação coulombiana), temperatura igual a zero, e sem incluir os efeitos da supercondutividade. É importante destacar que essas considerações foram feitas apenas no deslocamento, as outras partes do problema foram tratadas considerando finitas, as quantidades citadas acima. Na segunda aproximação, o deslocamento de banda foi estudado incluíndo-se os efeitos de U, da temperatura e da supercondutividade. Nos dois casos observou-se que a hibridização atua no sentido de suprimir a supercondutividade. Verificou-se também que na primeira aproximação considerada no cálculo do deslocamento de banda, os efeitos da hibridização sobre a supercondutividade, são superestimados. Isso ocorre porque certas funções correlação presentes no deslocamento de banda desaparecem no limite U →¥. Na segunda parte deste trabalho, incluiu-se no modelo, um termo de salto ( hopping ) para os segundos vizinhos de um sítio i. Esse termo foi ncluído com o objetivo de reproduzir adequadamente as assimetrias entre o regime de dopagem por buracos e o regime de dopagem por elétrons. No caso particular de dopagem por buracos, observou-se que a mudança na natureza da superfície de Fermi de buraco para elétron, profundamente afetada pela hibridização. Além disso, verificou-se que o efeito da hibridização ´e mais intenso nas regiões dos pontos de sela, nas quais o gap supercondutor ´e máximo devido à simetria dx2−y2 . Com isso, a temperatura crítica de transiçãoo (Tc) do estado normal para o estado supercondutor, também é afetada pela hibridização. Os resultados sugerem também, que no caso de dopagem por buracos, a hibridização interfere no valor de dopagem em que ocorre a mudança no sinal do coeficiente Hall, portanto deve afetar também as propriedades de transportes do sistema. No caso de dopagem por elétrons, não foi verificado nenhuma mudança na natureza da superf´ıcie de Fermi. No entanto, como a hibridização aumenta a largura das bandas e dimimui a densidade de estados no nível de Fermi, neste caso a supercondutividade também é afetada. A topologia da superfície de Fermi nos regimes de dopagen por buracos e por elétrons concorda bem com resultados experimentais de ARPES obtidos recentemente para o composto La2−xSrxCuO4 (dopado por buracos) e para o composto Nd2−xCexCuO4 (dopado por elétrons).
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Pecinatto, Henrique. "Efeitos da paridade da hibridização em supercondutores multibandas." Universidade Federal do Amazonas, 2017. http://tede.ufam.edu.br/handle/tede/5710.

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Recent studies have shown that antisymmetric hybridization favors the formation of bound states among the quasiparticles, and this is the main mechanism of the enhancement of the superconductivity in two-band systems. In this work, we study a generic two-band superconducting system, which forms intraband Cooper pairs and a term that’s responsible for the creation of Cooper’ pairs formed by fermions from different bands. The hybridization plays the role to transfer the quasiparticles among the bands and it can be tuned experimentally by an external factor like pressure or doping, allowing to explore the phase diagrams. Thus, the model is quite general, and we have the proposal to discuss the differences that hybridization parity acts in the purely intraband and interband case. The excitation energies as well the gap equation are obtained using the Green function’s method, and it had found the purely intraband case is no dependent of the hybridization parity. In the case of symmetric hybridization, the intraband case shows a discontinuity in the order parameter, a fact that doesn’t occur in the antisymmetric hybridization.
Estudos recentes tem mostrado que a hibridização antissimétrica favorece a formação de estados ligados entre as quasipartículas, e este é o principal mecanismo para o aumento da supercondutividade em sistemas de duas bandas. Neste trabalho, é estudado um sistema supercondutor de duas bandas genéricas com a formação de pares de Cooper intrabanda e um termo interbanda responsável pela criação de pares de Cooper formados por férmions de diferentes bandas. A hibridização tem o papel de transferir as quasipartículas entre as bandas e pode ser experimentalmente ajustado por um fator externo tal como a pressão ou dopagem, permitindo explorar os diagramas de fase. Assim, tem-se um modelo bastante geral, com a proposta de abordar as diferenças que a paridade da hibridização infere nos casos puramente intrabanda e interbanda. As energias de excitações bem como as equações de gap são obtidas por meio do método das funções de Green, e é encontrado que os casos puramente intrabanda independem da paridade da hibridização. Para a hibridização simétrica, o caso interbanda apresenta uma descontinuidade no parâmetro de ordem, fato que não ocorre na hibridização antissimétrica.
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Sola, I., S. Castellà, E. Viayna, C. Galdeano, M. C. Taylor, Stephen Y. Gbedema, B. Pérez, et al. "Synthesis, biological profiling and mechanistic studies of 4-aminoquinoline-based heterodimeric compounds with dual trypanocidal–antiplasmodial activity." 2015. http://hdl.handle.net/10454/7485.

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Yes
Dual submicromolar trypanocidal–antiplasmodial compounds have been identified by screening and chemical synthesis of 4-aminoquinoline-based heterodimeric compounds of three different structural classes. In Trypanosoma brucei, inhibition of the enzyme trypanothione reductase seems to be involved in the potent trypanocidal activity of these heterodimers, although it is probably not the main biological target. Regarding antiplasmodial activity, the heterodimers seem to share the mode of action of the antimalarial drug chloroquine, which involves inhibition of the haem detoxification process. Interestingly, all of these heterodimers display good brain permeabilities, thereby being potentially useful for late stage human African trypanosomiasis. Future optimization of these compounds should focus mainly on decreasing cytotoxicity and acetylcholinesterase inhibitory activity.

Book chapters on the topic "Dual hybridization":

1

Hauptmann, Giselbert, Gilbert Lauter, and Iris Söll. "Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish." In In Situ Hybridization Methods, 393–404. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2303-8_20.

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Kojima, Chiaki, and Noriko Funayama. "In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis." In Methods in Molecular Biology, 335–46. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_17.

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AbstractSponges (Porifera) are a large phylum that includes an enormous number of species. They are classified into four classes. Among these four classes, class Demospongiae is the largest and contains more than 90% of sponge species. In the last decade, methodologies for molecular studies and sequencing resources in sponge biology have dramatically advanced and made it possible to clearly define particular types of cells based on the genes they are expressing. Here we describe in detail the method of high-resolution WISH (whole mount in situ hybridization) and dual color fluorescent detection of in situ hybridization (dual color FISH) that we have established to detect particular types of cells, especially their stem cells known as archeocytes, in juveniles of freshwater demosponge, E. fluviatilis.
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Wang, Hongwei, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielsen, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma. "Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope." In Methods in Molecular Biology, 139–49. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1459-3_12.

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He, Nongyue, Song Li, and Hongna Liu. "High-Throughput SNP Detection Based on PCR Amplification on Magnetic Nanoparticles Using Dual-Color Hybridization." In Methods in Molecular Biology, 393–402. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-411-1_24.

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Pals, Gerard. "Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes." In Rapid Cycle Real-Time PCR — Methods and Applications, 77–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-59397-0_9.

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Petersen, Sandra L., and Sue McCrone. "Characterization of the Receptor Complement of Individual Neurons Using Dual-Label In Situ Hybridization Histochemistry." In In Situ Hybridization in Neurobiology, 78–95. Oxford University PressNew York, NY, 1994. http://dx.doi.org/10.1093/oso/9780195075076.003.0005.

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Abstract The availability of nonisotopic methods to detect specific mRNA sequences has facilitated the development of dual-label in situ hybridization histochemistry (ISHH). In this chapter we describe a very sensitive dual-label ISHH method which uses a combination of cRNA probes prepared by in vitro transcription of two different cDNA fragments, one in the presence of S-UTP and the other in the presence of digoxigenin-UTP. Our focus is on how dual-label ISHH can be used in conjunction with other methods to determine how individual neurons or specific populations of cells are regulated transsynaptically.
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Fähnrich, B., and A. Körner. "Easy Integration of 48V Mild Hybridization by Dual Voltage Battery Management – Realizing CO2 saving potentials by low implementation efforts." In ELIV 2019, 139–52. VDI Verlag, 2019. http://dx.doi.org/10.51202/9783181023570-139.

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Conference papers on the topic "Dual hybridization":

1

Song, Mingue, and Yanggon Kim. "Dual hybridization method for the classification of ultrasound breast tumors." In SAC '22: The 37th ACM/SIGAPP Symposium on Applied Computing. New York, NY, USA: ACM, 2022. http://dx.doi.org/10.1145/3477314.3507005.

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Zhou, Xue, Pengqi Gong, Shankun Wang, Yanan Zhang, Yong Zhao, Linh Viet Nguyen, Stephen C. Warren-Smith, and Xuegang Li. "In situ DNA hybridization detection using dual-channel optical fiber sensor with temperature compensation." In CLEO: Applications and Technology. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleo_at.2022.atu4k.7.

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We demonstrate, for the first time to our knowledge, the dual parameter measurement of temperature and DNA sequence by simultaneously using SPR and MZI in a simple single mode fiber-no-core fiber-SMF structure.
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Brenes, David, Qinghua Han, Kevin Bishop, Adam Glaser, and Jonathan T. C. Liu. "An open-top light-sheet (OTLS) microscope for surveying gene expression in thick expanded tissues." In Optics and the Brain. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/brain.2024.bs5c.4.

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We designed a novel non-orthogonal dual-objective open-top light-sheet microscope to resolve individual genomic/transcriptomic targets in thick hydrogel-based expanded fluorescence in situ hybridization labeled tissues at moderate tissue expansions.
4

Tsourkas, Andrew, Jason Xu, and Gang Bao. "Hybridization Dynamics and Kinetics of Fret-Enhanced Molecular Beacons." In ASME 2001 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2001. http://dx.doi.org/10.1115/imece2001/bed-23163.

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Abstract Many human diseases start with a defect in the genome. Cancer, for example, is a genetic disease that arises from a single cell that behaves abnormally, dividing uncontrollably and leading, eventually, to the development of a tumor. A critical step in diagnosing and treating cancer is to detect cancer cells that result from the mutated genes. In spite of the extensive biomedical research efforts during the last few decades, it is still difficult to detect cancer at its early stages — when a cancer is diagnosed it is often too late to cure. A novel way of achieving early detection of cancer is to detect mRNA transcripts that arise from mutated genes in living cells [1]. We have developed a FRET-enhanced molecular beacons methodology which, combined with the state-of-the-art fluorescence imaging techniques, has the potential to detect cancer cells. FRET (Fluorescence resonance energy transfer) refers to the non-radiative transfer of energy from a donor molecule to an acceptor molecule through dipole-dipole coupling. As shown schematically in Figure 1, molecular beacons are dual labeled antisense oligonucleotides (ODNs) with a fluorophore (A or D) at one end and a quencher (Q) at the other; they are designed to form a hairpin structure in the absence of a complimentary target such that fluorescence of the fluorophore is quenched. Upon hybridization with the target mRNA, the molecular beacon opens up, leading to fluorescence [2,3].
5

Kalathakis, C., N. Aretakis, I. Roumeliotis, A. Alexiou, and K. Mathioudakis. "Investigation of Different Solar Hybrid Gas Turbines and Exploitation of Rejected Sun Power." In ASME Turbo Expo 2016: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/gt2016-57700.

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Solar hybrid gas turbine performance is studied through consideration of four engine configurations: a) single shaft, b) recuperated single-shaft, c) twin-shaft and d) two-spool three-shaft, intercooled, recuperated. For each configuration and for the same design point, the performance is obtained for two hybridization schemes: Fuel only engines Retrofitted for Solar operation (FRS) and engines designed with Solar only operation at the Design Point (SDP). In an attempt to further improve the benefits of hybridization, the concept of a Dual Fluid Receiver for exploiting the rejected solar power, during sunny hours with high irradiation, is demonstrated. Steam is produced by focusing the defocused mirrors of the heliostat field to a second receiver and injected into the combustion chamber. For the cases examined, it can be concluded that FRS engines show better performance than SDP ones, since they exhibit higher thermal fuel efficiency and higher specific power. Concerning the configurations, an annual fuel saving of ∼35% and an annual output reduction, ranging from 4% for the recuperated single-shaft configuration to 9% for the twin shaft configuration compared to the corresponding fuel-only engines is demonstrated. The inclusion of a Dual Fluid Receiver in an FRS engine removes the power penalty while it maintains the fuel saving benefit.
6

Jialong Peng, Xiaodong Yuan, Zhihong Zhu, Jianfa Zhang, and Shiqiao Qin. "Dual-frequency tunable terahertz half-wave plate based on coupling and hybridization effect in graphene nanodisk dimers." In 2016 Progress in Electromagnetic Research Symposium (PIERS). IEEE, 2016. http://dx.doi.org/10.1109/piers.2016.7735453.

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Niikura, N., N. Kumaki, T. Iwamoto, B. Tsuda, T. Okamura, S. Yuki, Y. Suzuki, and Y. Tokuda. "Abstract P2-05-08: Determination of HER2 amplification by dual-color in situ hybridization before and after neoadjuvant chemotherapy." In Abstracts: Thirty-Fifth Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 4‐8, 2012; San Antonio, TX. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/0008-5472.sabcs12-p2-05-08.

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Le Lepvrier, B., R. Loison, R. Gillard, L. Patier, P. Potier, and P. Pouliguen. "Hybridization of the Dual-Grid FDTD with the physical optics to analyze antennas mounted on large and complex platforms." In 2013 International Conference on Electromagnetics in Advanced Applications (ICEAA). IEEE, 2013. http://dx.doi.org/10.1109/iceaa.2013.6632241.

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Mansfield, AS, Y. Sakai, FJ Walsh, AE Wiktor, WR Sukov, A. Dogan, and RB Jenkins. "Abstract PD02-05: Comparison of fluorescence in situ hybridization (FISH) and dual-ISH (D-ISH) in the determination of HER2 status." In Abstracts: Thirty-Fifth Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 4‐8, 2012; San Antonio, TX. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/0008-5472.sabcs12-pd02-05.

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Raut, Abhinandan, Suryaji Phalke, and Diane Peters. "Modeling and Control of New Multi-Mode Plug-in Hybrid Electric Vehicle." In ASME 2019 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/detc2019-97660.

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Abstract Fuel economy and emission standards for internal combustion engine (ICE) vehicles lead to emergence of hybrid powertrain mechanisms. Hybrid powertrains can enable significant fuel economy improvements without sacrificing vehicle performance or utility. This requires optimization of engine operation, regenerative braking, and use of a wide range of possible combinations of engine and battery usage. The multi-mode hybrid powertrain in this paper combines many options to meet a complex driving requirement while maintaining the desired fuel economy. In this paper, a systematic design methodology is used to design a full-size hybrid vehicle with multiple components. This involves the modeling, simulation and development of optimal energy management strategy. This vehicle (full size car) has dual battery, dual fuel V6 engine with cylinder deactivation and bi-directional power flow in and from dual motor/generator. The design includes multiple gearboxes to connect these pieces. The vehicle model allows many degrees of freedom including various modes of operation depending upon the combination of degree of driver involvement, vehicle power requirement and optimized fuel economy resulting in automatic switching between modes. This model is tested for different Environmental Protection Agency (EPA) driving cycles. By integrating all components of this hybrid electric vehicle (HEV) and the highly coordinated energy management control system that performs optimum blending of torque, speed, and power from multiple power sources, the benefit from this hybridization is maximized.

Reports on the topic "Dual hybridization":

1

Sessa, Guido, and Gregory Martin. A functional genomics approach to dissect resistance of tomato to bacterial spot disease. United States Department of Agriculture, January 2004. http://dx.doi.org/10.32747/2004.7695876.bard.

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The research problem. Bacterial spot disease in tomato is of great economic importance worldwide and it is particularly severe in warm and moist areas affecting yield and quality of tomato fruits. Causal agent of spot disease is the Gram-negative bacterium Xanthomonas campestris pv. vesicatoria (Xcv), which can be a contaminant on tomato seeds, or survive in plant debris and in association with certain weeds. Despite the economic significance of spot disease, plant protection against Xcvby cultural practices and chemical control have so far proven unsuccessful. In addition, breeding for resistance to bacterial spot in tomato has been undermined by the genetic complexity of the available sources of resistance and by the multiple races of the pathogen. Genetic resistance to specific Xcvraces have been identified in tomato lines that develop a hypersensitive response and additional defense responses upon bacterial challenge. Central goals of this research were: 1. To identify plant genes involved in signaling and defense responses that result in the onset of resistance. 2. To characterize molecular properties and mode of action of bacterial proteins, which function as avirulence or virulence factors during the interaction between Xcvand resistant or susceptible tomato plants, respectively. Our main achievements during this research program are in three major areas: 1. Identification of differentially expressed genes during the resistance response of tomato to Xcvrace T3. A combination of suppression subtractive hybridization and microarray analysis identified a large set of tomato genes that are induced or repressed during the response of resistant plants to avirulent XcvT3 bacteria. These genes were grouped in clusters based on coordinate expression kinetics, and classified into over 20 functional classes. Among them we identified genes that are directly modulated by expression of the type III effector protein AvrXv3 and genes that are induced also during the tomato resistance response to Pseudomonas syringae pv. tomato. 2. Characterization of molecular and biochemical properties of the tomato LeMPK3MAP kinase. A detailed molecular and biochemical analysis was performed for LeMPK3 MAP kinase, which was among the genes induced by XcvT3 in resistant tomato plants. LeMPK3 was induced at the mRNA level by different pathogens, elicitors, and wounding, but not by defense-related plant hormones. Moreover, an induction of LeMPK3 kinase activity was observed in resistant tomato plants upon Xcvinfection. LeMPK3 was biochemically defined as a dual-specificity MAP kinase, and extensively characterized in vitro in terms of kinase activity, sites and mechanism of autophosphorylation, divalent cation preference, Kₘand Vₘₐₓ values for ATP. 3. Characteriztion of molecular properties of the Xcveffector protein AvrRxv. The avirulence gene avrRxvis involved in the genetic interaction that determines tomato resistance to Xcvrace T1. We found that AvrRxv functions inside the plant cell, localizes to the cytoplasm, and is sufficient to confer avirulence to virulent Xcvstrains. In addition, we showed that the AvrRxv cysteine protease catalytic core is essential for host recognition. Finally, insights into cellular processes activated by AvrRxv expression in resistant plants were obtained by microarray analysis of 8,600 tomato genes. Scientific and agricultural significance: The findings of these activities depict a comprehensive and detailed picture of cellular processes taking place during the onset of tomato resistance to Xcv. In this research, a large pool of genes, which may be involved in the control and execution of plant defense responses, was identified and the stage is set for the dissection of signaling pathways specifically triggered by Xcv.

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