Relevant bibliographies by topics / DsbD

Academic literature on the topic 'DsbD'

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Published: 10 December 2022
Last updated: 29 July 2024

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Journal articles on the topic "DsbD"

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Cho, Seung-Hyun, and Jon Beckwith. "Mutations of the Membrane-Bound Disulfide Reductase DsbD That Block Electron Transfer Steps from Cytoplasm to Periplasm in Escherichia coli." Journal of Bacteriology 188, no. 14 (July 15, 2006): 5066–76. http://dx.doi.org/10.1128/jb.00368-06.

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ABSTRACT The cytoplasmic membrane protein DsbD keeps the periplasmic disulfide isomerase DsbC reduced, using the cytoplasmic reducing power of thioredoxin. DsbD contains three domains, each containing two reactive cysteines. One membrane-embedded domain, DsbDβ, transfers electrons from thioredoxin to the carboxy-terminal thioredoxin-like periplasmic domain DsbDγ. To evaluate the role of conserved amino acid residues in DsbDβ in the electron transfer process, we substituted alanines for each of 19 conserved amino acid residues and assessed the in vivo redox states of DsbC and DsbD. The mutant DsbDs of 11 mutants which caused defects in DsbC reduction showed relatively oxidized redox states. To analyze the redox state of each DsbD domain, we constructed a thrombin-cleavable DsbD (DsbDTH) from which we could generate all three domains as separate polypeptide chains by thrombin treatment in vitro. We divided the mutants with strong defects into two classes. The first mutant class consists of mutant DsbDβ proteins that cannot receive electrons from cytoplasmic thioredoxin, resulting in a DsbD that has all six of its cysteines disulfide bonded. The second mutant class represents proteins in which the transfer of electrons from DsbDβ to DsbDγ appears to be blocked. This class includes the mutant with the most clear-cut defect, P284A. We relate the properties of the mutants to the positions of the amino acids in the structure of DsbD and discuss mechanisms that would interfere with the electron transfer process.
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Walden, Patricia M., Andrew E. Whitten, Lakshmanane Premkumar, Maria A. Halili, Begoña Heras, Gordon J. King, and Jennifer L. Martin. "The atypical thiol–disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase." Acta Crystallographica Section D Structural Biology 75, no. 3 (February 26, 2019): 283–95. http://dx.doi.org/10.1107/s2059798318018442.

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Disulfide-bond-forming (DSB) oxidative folding enzymes are master regulators of virulence that are localized to the periplasm of many Gram-negative bacteria. The archetypal DSB machinery from Escherichia coli K-12 consists of a dithiol-oxidizing redox-relay pair (DsbA/B), a disulfide-isomerizing redox-relay pair (DsbC/D) and the specialist reducing enzymes DsbE and DsbG that also interact with DsbD. By contrast, the Gram-negative bacterium Wolbachia pipientis encodes just three DSB enzymes. Two of these, α-DsbA1 and α-DsbB, form a redox-relay pair analogous to DsbA/B from E. coli. The third enzyme, α-DsbA2, incorporates a DsbA-like sequence but does not interact with α-DsbB. In comparison to other DsbA enzymes, α-DsbA2 has ∼50 extra N-terminal residues (excluding the signal peptide). The crystal structure of α-DsbA2ΔN, an N-terminally truncated form in which these ∼50 residues are removed, confirms the DsbA-like nature of this domain. However, α-DsbA2 does not have DsbA-like activity: it is structurally and functionally different as a consequence of its N-terminal residues. Firstly, α-DsbA2 is a powerful disulfide isomerase and a poor dithiol oxidase: i.e. its role is to shuffle rather than to introduce disulfide bonds. Moreover, small-angle X-ray scattering (SAXS) of α-DsbA2 reveals a homotrimeric arrangement that differs from those of the other characterized bacterial disulfide isomerases DsbC from Escherichia coli (homodimeric) and ScsC from Proteus mirabilis (PmScsC; homotrimeric with a shape-shifter peptide). α-DsbA2 lacks the shape-shifter motif and SAXS data suggest that it is less flexible than PmScsC. These results allow conclusions to be drawn about the factors that are required for functionally equivalent disulfide isomerase enzymatic activity across structurally diverse protein architectures.
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Lin, Dongxia, Byoungkwan Kim, and James M. Slauch. "DsbL and DsbI contribute to periplasmic disulfide bond formation in Salmonella enterica serovar Typhimurium." Microbiology 155, no. 12 (December 1, 2009): 4014–24. http://dx.doi.org/10.1099/mic.0.032904-0.

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Disulfide bond formation in periplasmic proteins is catalysed by the DsbA/DsbB system in most Gram-negative bacteria. Salmonella enterica serovar Typhimurium also encodes a paralogous pair of proteins to DsbA and DsbB, DsbL and DsbI, respectively, downstream of a periplasmic arylsulfate sulfotransferase (ASST). We show that DsbL and DsbI function as a redox pair contributing to periplasmic disulfide bond formation and, as such, affect transcription of the Salmonella pathogenicity island 1 (SPI1) type three secretion system genes and activation of the RcsCDB system, as well as ASST activity. In contrast to DsbA/DsbB, however, the DsbL/DsbI system cannot catalyse the disulfide bond formation required for flagellar assembly. Phylogenic analysis suggests that the assT dsbL dsbI genes are ancestral in the Enterobacteriaceae, but have been lost in many lineages. Deletion of assT confers no virulence defect during acute Salmonella infection of mice.
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Skórko-Glonek, Joanna, Anna Sobiecka-Szkatuła, and Barbara Lipińska. "Characterization of disulfide exchange between DsbA and HtrA proteins from Escherichia coli." Acta Biochimica Polonica 53, no. 3 (October 1, 2006): 585–89. http://dx.doi.org/10.18388/abp.2006_3331.

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DsbA is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. In the present work we provided the first detailed characterization of disulfide exchange between DsbA and its natural substrate, HtrA protease. We demonstrated that HtrA oxidation relies on DsbA, both in vivo and in vitro. We followed the disulfide exchange between these proteins spectrofluorimetrically and found that DsbA oxidizes HtrA with a 1:1 stoichiometry. The calculated second-order apparent rate constant (kapp) of this reaction was 3.3x10(4)+/-0.6x10(4) M-1s-1. This value was significantly higher than the values obtained for nonfunctional disulfide exchanges between DsbA and DsbC or DsbD and it was comparable to the kapp values calculated for in vitro oxidation of certain non-natural DsbA substrates of eukaryotic origin.
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Kurokawa, Yoichi, Hideki Yanagi, and Takashi Yura. "Overexpression of Protein Disulfide Isomerase DsbC Stabilizes Multiple-Disulfide-Bonded Recombinant Protein Produced and Transported to the Periplasm in Escherichia coli." Applied and Environmental Microbiology 66, no. 9 (September 1, 2000): 3960–65. http://dx.doi.org/10.1128/aem.66.9.3960-3965.2000.

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ABSTRACT Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm ofEscherichia coli. By using a set of Dsb coexpression plasmids constructed recently, we analyzed the effects of Dsb overexpression on production of horseradish peroxidase (HRP) isozyme C that contains complex disulfide bonds and tends to aggregate when produced in E. coli. When transported to the periplasm, HRP was unstable but was markedly stabilized upon simultaneous overexpression of the set of Dsb proteins (DsbABCD). Whereas total HRP production increased severalfold upon overexpression of at least disulfide-bonded isomerase DsbC, maximum transport of HRP to the periplasm seemed to require overexpression of all DsbABCD proteins, suggesting that excess Dsb proteins exert synergistic effects in assisting folding and transport of HRP. Periplasmic production of HRP also increased when calcium, thought to play an essential role in folding of nascent HRP polypeptide, was added to the medium with or without Dsb overexpression. These results suggest that Dsb proteins and calcium play distinct roles in periplasmic production of HRP, presumably through facilitating correct folding. The present Dsb expression plasmids should be useful in assessing and dissecting periplasmic production of proteins that contain multiple disulfide bonds in E. coli.
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Goldstone, D., P. W. Haebel, F. Katzen, M. W. Bader, J. C. A. Bardwell, J. Beckwith, and P. Metcalf. "DsbC activation by the N-terminal domain of DsbD." Proceedings of the National Academy of Sciences 98, no. 17 (August 7, 2001): 9551–56. http://dx.doi.org/10.1073/pnas.171315498.

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Smith, Roxanne P., Biswaranjan Mohanty, Shakeel Mowlaboccus, Jason J. Paxman, Martin L. Williams, Stephen J. Headey, Geqing Wang, et al. "Structural and biochemical insights into the disulfide reductase mechanism of DsbD, an essential enzyme for neisserial pathogens." Journal of Biological Chemistry 293, no. 43 (September 4, 2018): 16559–71. http://dx.doi.org/10.1074/jbc.ra118.004847.

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The worldwide incidence of neisserial infections, particularly gonococcal infections, is increasingly associated with antibiotic-resistant strains. In particular, extensively drug-resistant Neisseria gonorrhoeae strains that are resistant to third-generation cephalosporins are a major public health concern. There is a pressing clinical need to identify new targets for the development of antibiotics effective against Neisseria-specific processes. In this study, we report that the bacterial disulfide reductase DsbD is highly prevalent and conserved among Neisseria spp. and that this enzyme is essential for survival of N. gonorrhoeae. DsbD is a membrane-bound protein that consists of two periplasmic domains, n-DsbD and c-DsbD, which flank the transmembrane domain t-DsbD. In this work, we show that the two functionally essential periplasmic domains of Neisseria DsbD catalyze electron transfer reactions through unidirectional interdomain interactions, from reduced c-DsbD to oxidized n-DsbD, and that this process is not dictated by their redox potentials. Structural characterization of the Neisseria n- and c-DsbD domains in both redox states provides evidence that steric hindrance reduces interactions between the two periplasmic domains when n-DsbD is reduced, thereby preventing a futile redox cycle. Finally, we propose a conserved mechanism of electron transfer for DsbD and define the residues involved in domain–domain recognition. Inhibitors of the interaction of the two DsbD domains have the potential to be developed as anti-neisserial agents.
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Yu, Jun. "Inactivation of DsbA, but Not DsbC and DsbD, Affects the Intracellular Survival and Virulence ofShigella flexneri." Infection and Immunity 66, no. 8 (August 1, 1998): 3909–17. http://dx.doi.org/10.1128/iai.66.8.3909-3917.1998.

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ABSTRACT In this study, three mutants,dsbA::kan, dsbC-kan, anddsbD-kan, of Shigella flexneri serotype 5 were constructed and characterized to investigate the role of the periplasmic thiol:disulfide oxidoreductases in pathogenicity. In gentamicin protection assays and the Serény test, thedsbA mutant showed reduced virulence while thedsbC and dsbD mutants were similar to the wild type. That inactivation of dsbA was responsible for the reduced virulence was verified by complementation with the cloned wild-type gene in in vitro and in vivo assays. Despite the changed virulence behavior, the dsbA mutant could penetrate HeLa cells 15 min postinfection, consistent with the fact that it actively secretes Ipa proteins upon Congo red induction. Furthermore, thedsbA mutant was able to produce actin comets and protrusions, indicating its capacity for intra- and intercellular spread. However, a kinetic analysis of intracellular growth showed that the dsbA mutant barely grew in HeLa cells during a 4-h infection whereas the wild type had a doubling time of 41 min. Electron microscopy analysis revealed that dsbA mutant bacteria were trapped in protrusion-derived vacuoles surrounded by double membranes, resembling an icsB mutant reported previously. Moreover, the trapped bacteria appeared to be lysed simultaneously with the double membranes, resulting in characteristic empty vacuoles in the host cell cytosol. Thus, the attenuation mechanism for dsbAmutant appears to be more complicated than was previously suggested.
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Kimball, Richard A., Laetitia Martin, and Milton H. Saier Jr. "Reversing Transmembrane Electron Flow: The DsbD and DsbB Protein Families." Journal of Molecular Microbiology and Biotechnology 5, no. 3 (2003): 133–49. http://dx.doi.org/10.1159/000070263.

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Smith, Roxanne P., Andrew E. Whitten, Jason J. Paxman, Charlene M. Kahler, Martin J. Scanlon, and Begoña Heras. "Production, biophysical characterization and initial crystallization studies of the N- and C-terminal domains of DsbD, an essential enzyme inNeisseria meningitidis." Acta Crystallographica Section F Structural Biology Communications 74, no. 1 (January 1, 2018): 31–38. http://dx.doi.org/10.1107/s2053230x17017800.

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The membrane protein DsbD is a reductase that acts as an electron hub, translocating reducing equivalents from cytoplasmic thioredoxin to a number of periplasmic substrates involved in oxidative protein folding, cytochromecmaturation and oxidative stress defence. DsbD is a multi-domain protein consisting of a transmembrane domain (t-DsbD) flanked by two periplasmic domains (n-DsbD and c-DsbD). Previous studies have shown that DsbD is required for the survival of the obligate human pathogenNeisseria meningitidis. To help understand the structural and functional aspects ofN. meningitidisDsbD, the two periplasmic domains which are required for electron transfer are being studied. Here, the expression, purification and biophysical properties of n-NmDsbD and c-NmDsbD are described. The crystallization and crystallographic analysis of n-NmDsbD and c-NmDsbD are also described in both redox states, which differ only in the presence or absence of a disulfide bond but which crystallized in completely different conditions. Crystals of n-NmDsbDOx, n-NmDsbDRed, c-NmDsbDOxand c-NmDsbDReddiffracted to 2.3, 1.6, 2.3 and 1.7 Å resolution and belonged to space groupsP213,P321,P41andP1211, respectively.
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Dissertations / Theses on the topic "DsbD"

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Mavridou, Despoina A. I. "Elucidation of the structure-function relationships in the bacterial transmembrane disulfide oxidoreductase DsbD." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.497048.

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Quinternet, Marc. "Analyse structurale et dynamique par RMN des domaines N-terminaux des protéines DsbD et PilB de Neisseria meningitidis et de leur interaction." Thesis, Vandoeuvre-les-Nancy, INPL, 2008. http://www.theses.fr/2008INPL102N/document.

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Nous montrons que la structure RMN, en solution, du domaine N-terminal de DsbD de Neisseria meningitidis (nDsbD) présente, à l’état réduit, un repliement de type immunoglobuline avec un site actif adoptant une conformation fermée. Toutefois, l’analyse des mouvements internes du squelette peptique de nDsbD montre que la région dite « couvercle » de la protéine et qui protège les résidus actifs dans les formes réduite et oxydée, est dotée de mouvements internes. Cela démontre les capacités intrinsèques d’ajustement structural de nDsbD. Nous montrons aussi que les structures RMN, en solution, du domaine N-terminal de PilB de N. meningitidis (NterPilB) sous ses deux formes, réduite et oxydée, présentent un repliement de type thiorédoxine. Ces deux formes, très proches d’un point de vue structural, apparaissent comme étant globalement rigides. Par conséquent, la boucle FLHE, caractéristique de NterPilB et bordant le site actif de la protéine, ne dévoile pas de nouveaux indices structuraux et/ou dynamiques traduisant son implication dans la spécificité de substrat. Finalement, nous montrons, grâce à l’étude structurale et dynamique, en solution, d’un complexe entre nDsbD et NterPilB de N. meningitidis, que nDsbD fait preuve d’une grande adaptabilité à l’état complexé. La région « couvercle » s’ouvre pour venir se positionner au dessus de l’hélice a qui contient les cystéines actives de NterPilB. Par contre, la boucle FLHE de NterPilB ne semble pas intervenir dans la stabilisation du complexe. Nous proposons que des phénomènes dynamiques puissent faciliter d’une part, l’adaptabilité relative des deux partenaires dans le complexe, et d’autre part, la dissociation finale de ces derniers
We show, on one hand, that the NMR solution structure of DsbD N-terminal domain from Neisseria meningitidis (nDsbD) displays, in its reduced state, an immunoglobulin fold with a closed conformation of its active site. Nonetheless, our backbone dynamics study shows that the cap-loop region of the protein, which covers active residues in both oxidized and reduced forms, displays internal motions. This illustrates the inner structural adjustment capacities of nDsbD. On the other hand, we show that NMR solution structures of the oxidized and reduced forms of N. meningitidis NterPilB display a thioredoxin-like fold. These two structures appear to be very similar and globally rigid. Consequently, the NterPilB characteristic FLHE loop, which covers one edge of the active site, does not reveal new structural and/or dynamics properties for its involvement in the substrate specificity. Finally, we point out, from the structural and dynamics study of a complex between nDsbD and NterPilB from N. meningitidis, that nDsbD exhibits a powerful adaptability in its complex state. Its cap-loop region opens and comes over the a helix containing the NterPilB active cysteines. In contrast, the NterPilB FLHE loop does not seem to play a role in the complex stabilization. We propose that internal dynamics should facilitate, on one hand, the relative adaptability between the two partners of the complex and, on the other hand, their subsequent dissociation
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Quinternet, Marc Cung Manh Thông. "Analyse structurale et dynamique par RMN des domaines N-terminaux des protéines DsbD et PilB de Neisseria meningitidis et de leur interaction." S. l. : S. n, 2008. http://www.scd.inpl-nancy.fr/theses/2008_QUINTERNET_M.pdf.

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Urban, Andreas. "Die Rolle der Thiol-Disulfid-Oxidoreduktasen DsbA und DsbC bei der Proteinsekretion in Pseudomonas aeruginosa." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=959986855.

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Frank, Lisa Lucie [Verfasser]. "Die Bedeutung der Proteine BamC, HlpA, DsbB, DsbH und DsbA1 für die Integrität der Außenmembran von Pseudomonas aeruginosa / Lisa Lucie Frank." Tübingen : Universitätsbibliothek Tübingen, 2020. http://d-nb.info/122345116X/34.

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Bhandari, Murari. "Investigating the role of DsbA enzymes in growth and virulence of uropathogenic Escherichia coli." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/120696/2/Murari_Bhandari_Thesis.pdf.

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This study set out to investigate the impact of inhibiting bacterial enzymes on pathogen growth and virulence as an alternative antimicrobial strategy to antibiotics. Results support the hypothesis that DsbA enzyme inhibition is a robust anti-virulence strategy that can 'disarm but not kill bacteria' using uropathogenic Escherichia coli as the model pathogen. These findings have enhanced the pharmacological importance of DsbA as an anti-virulence drug target and contribute to ongoing research that aims to develop DsbA inhibitors against different bacterial pathogens with diverse DsbA enzymes.
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Hittel, Dustin S. "Overexpression of the dsvD gene of Desulfovibrio vulgaris Hildenborough and characterization of the DsvD protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ38590.pdf.

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Mitta, Ever. "Consulting report – DSB Mobile." Master's thesis, Pontificia Universidad Católica del Perú, 2017. http://tesis.pucp.edu.pe/repositorio/handle/123456789/9409.

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DSB Mobile is a small Peruvian software developing company based in Lima. DSB Mobile specializes in the development of both mobile and web applications and has worked with major companies such as Samsung, Claro & Entel. The company is composed of the General Manager, Zico Herrera, a sales manager, operations manager and both fulltime and contract based software developers that are hired based on the current demand for service. DSB Mobile has established a strong reputation and brand in Peru and is now looking to expand outside of Peru where they can introduce their software products into international markets. In their aspiration to internationalize, DSB Mobile is seeking to find out not only the most profitable markets for their company but also markets that would best align with DSB Mobiles mission. The solution to their question of expansion was to determine the best markets using a variety of both quantitative and qualitative factors. In using an IT Competitiveness report that was done by the British Software Alliance this was used as a reference point to determine the best ranked countries for IT competitiveness and the best countries to conduct business in base off of important IT indicators. Combined with software data statistics in terms of charges per project and cost of IT Consultants, this further narrowed down the scope to the most attractive, profitable and mutually beneficial market for DSB Mobile. The implementation plan proposed involved two market lines namely, the North American market line and the European Market line. The proposed solution takes into consideration different scenarios, one of them being the moderate work balance of 1 project per month, giving a total cost of $391,065 per year with a profit of $180,736. The Gantt chart outlined is intended to guide the company with the step by step implementation of this international expansion and prepare them to execute this plan in the most efficient and effective manner
DSB Mobile es una pequeña empresa peruana de desarrollo de software con sede en Lima. DSB Mobile se especializa en el desarrollo de aplicaciones móviles y web y ha trabajado con grandes empresas como Samsung, Claro y Entel. La compañía está compuesta por el Gerente General, Zico Herrera, un gerente de ventas, un gerente de operaciones y desarrolladores de software a tiempo completo y algunos que se contratan en base a la demanda actual de servicio. DSB Mobile ha establecido una fuerte reputación y marca en Perú y ahora está buscando expandirse fuera de Perú donde puedan introducir sus productos de software en los mercados internacionales. En su aspiración de internacionalización, DSB Mobile está tratando de descubrir no sólo los mercados más rentables para su empresa, sino también los mercados que mejor se alinean con la misión DSB Mobile. La solución a su problema de expansión fue determinar los mejores mercados utilizando una variedad de factores tanto cuantitativos como cualitativos. Al utilizar un informe de competitividad de TI que fue realizado por la British Software Alliance, se utilizó como punto de referencia para determinar los países mejor clasificados para la competitividad de TI y los mejores países para llevar a cabo negocios en base de importantes indicadores asociados a estos. Combinado con estadísticas de datos de software en términos de gastos por proyecto y coste de consultores en TI, esto permitió reducir aún más el alcance para obtener un mercado más atractivo, rentable y mutuamente beneficioso. El plan de implementación propuesto involucró dos líneas de mercado, a saber, la línea del mercado norteamericano y la línea del mercado europeo. La solución propuesta posee diferentes escenarios; por ejemplo, el escenario con trabajo moderado consta de 1 proyecto por mes y tiene un costo total de $391,065 por año obteniendo así una rentabilidad de $180,736. El gráfico de Gantt esbozado pretende guiar a la compañía con la implementación paso a paso de esta expansión internacional y prepararlos para ejecutar este plan de la manera más eficiente y efectiva
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Mota, Lúcia Santiago. "DSD: interfaces e interacções." Master's thesis, Universidade de Aveiro, 2009. http://hdl.handle.net/10773/4531.

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Mestrado em Engenharia de Computadores e Telemática
As ferramentas de Front e Back Office baseadas em serviços Web são actualmente uma realidade comum. Estas fornecem ao utilizador, independentemente do local ou mesmo do terminal, dentro de certos contextos, uma interface única e diferenciada consoante o perfil do utilizador. Para cada perfil, estas interfaces fornecem unicamente o conjunto de comandos e informações necessárias. A plataforma de DSD, onde já se gerem muitas das informações que se relacionam com o processo de manutenção do DETI, tem múltiplos utilizadores com perfis e objectivos muito diferentes. Este documento começa com um estudo do estado inicial da plataforma existente de forma a identificar toda a sua funcionalidade. Posteriormente, é realizada uma análise das novas tarefas a implementar, utilizadores e respectivo modelo de domínio de forma a conhecer toda a nova base estrutural da plataforma. Finalmente é analisada a interface da plataforma a nível de usabilidade para garantir a satisfação dos utilizadores. Toda a plataforma está desenvolvida a pensar em futuras evoluções para continuar a evoluir e trazer valor à organização interna dos departamentos universitários.
The tools of Front and Back Office based on Web Services are nowadays a common reality. These give the user, regardless of location or terminal, within a certain context, a single interface that varies depending of the user profile. For each profile, these interfaces provide only the necessary set of commands and information. The DSD platform, which is already generating a lot of information, related with the managing process of DETI, has multiple users with very different profiles and objectives. The work begins with a study of the initial state of the existing platform with the objective of identifying the available functionality. Furthermore, all the new tasks, users and domain model are analyzed in order to define the new structural basis of the platform. Finally, an usability study of the user interface of the platform is performed in order to obtain an acceptable usability level and ensure user satisfaction. The entire platform is developed considering future developments, in order to continue evolving and bringing value to the internal organization of university departments.
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Mizuno, Nobuhiro. "Structure-based functional analysis of DsrD protein." 京都大学 (Kyoto University), 2004. http://hdl.handle.net/2433/147845.

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Books on the topic "DsbD"

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Banetjenesten, Danske statsbaner, ed. DSB baneanlæg. [Copenhagen]: Teknisk afdeling, 1989.

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Philippines. Dept. of Social Welfare and Development. DSWD: 60 years of heart and soul. [Manila]: Dept. of Social Welfare and Development, 2010.

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Economie, de Volkskrant Redactie, ed. Opkomst en ondergang van DSB. Schoorl: Conserve, 2009.

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Loor, André. Verbonden: Suriname en DSB, 1865-1990. Paramaribo, Suriname: Surinaamsche Bank, 1990.

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Kjeld, Heltoft, ed. DSB plakater 1975-84: Kunsternerne fortæller. [Copenhagen]: DBK, 1985.

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Brandt, Mette. Hvordan jeg lærte at elske DSB. Århus: Klim, 1988.

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Steffensen, Erik. Pas paa toget!: 25 års DSB-plakatkunst. København: Arkitektens forlag, 2001.

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Mohamad, Gunawan. Debu, duka, dsb: Sebuah pertimbangan anti-theodise. [Jakarta]: Tempo, 2011.

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Walker, R. J. Determination of lead in sea water by ICP-AES using DSID. Manchester: UMIST, 1994.

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Pedersen, Ole Nørregaard. Operation genforening: Statsbanerne og Sønderjylland 1918-1928. København: Danmarks jernbanemuseum, 2001.

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Book chapters on the topic "DsbD"

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Roen, Katrina. "Intersex/DSD." In The Palgrave Handbook of the Psychology of Sexuality and Gender, 183–97. London: Palgrave Macmillan UK, 2015. http://dx.doi.org/10.1057/9781137345899_12.

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Bartlett, Murray. "Imaging in DSD." In Disorders of Sex Development, 133–45. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22964-0_14.

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Hutson, John M., and Chris Kimber. "Laparoscopy for DSD." In Disorders of Sex Development, 183–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22964-0_18.

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Hewitt, Jacqueline K., and Garry L. Warne. "46,XY DSD." In Disorders of Sex Development, 63–80. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22964-0_7.

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Hutson, John M. "Non-hormonal DSD." In Disorders of Sex Development, 89–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22964-0_9.

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Hutson, John M., and Aurore Bouty. "Embryology in DSD." In Disorders|Differences of Sex Development, 49–64. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-7864-5_5.

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O’Connell, Michele A., Sonia R. Grover, and Aurore Bouty. "46,XX DSD." In Disorders|Differences of Sex Development, 65–75. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-7864-5_6.

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O’Connell, Michele A., Aurore Bouty, and Sonia R. Grover. "46,XY DSD." In Disorders|Differences of Sex Development, 77–102. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-7864-5_7.

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O’Connell, Michele A. "Sex Chromosome DSD." In Disorders|Differences of Sex Development, 103–13. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-7864-5_8.

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Hutson, John M., and Aurore Bouty. "Non-hormonal DSD." In Disorders|Differences of Sex Development, 115–22. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-13-7864-5_9.

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Conference papers on the topic "DsbD"

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Li, Renqiang, Hong Liu, Xiangdong Wang, and Yueliang Qian. "DSBI." In ICVIP 2018: 2018 the 2nd International Conference on Video and Image Processing. New York, NY, USA: ACM, 2018. http://dx.doi.org/10.1145/3301506.3301532.

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Zhang, Haoxiang, Aécio Santos, and Juliana Freire. "DSDD." In CIKM '21: The 30th ACM International Conference on Information and Knowledge Management. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3459637.3482427.

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Klarlund, Nils, Anders Moller, and Michael I. Schwartzbach. "DSD." In the third workshop. New York, New York, USA: ACM Press, 2000. http://dx.doi.org/10.1145/349360.351158.

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Liu, Wenge, Yi Cheng, Hao Wang, Jianheng Tang, Yafei Liu, Ruihui Zhao, Wenjie Li, Yefeng Zheng, and Xiaodan Liang. "“My nose is running.” “Are you also coughing?”: Building A Medical Diagnosis Agent with Interpretable Inquiry Logics." In Thirty-First International Joint Conference on Artificial Intelligence {IJCAI-22}. California: International Joint Conferences on Artificial Intelligence Organization, 2022. http://dx.doi.org/10.24963/ijcai.2022/592.

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With the rise of telemedicine, the task of developing Dialogue Systems for Medical Diagnosis (DSMD) has received much attention in recent years. Different from early researches that needed to rely on extra human resources and expertise to build the system, recent researches focused on how to build DSMD in a data-driven manner. However, the previous data-driven DSMD methods largely overlooked the system interpretability, which is critical for a medical application, and they also suffered from the data sparsity issue at the same time. In this paper, we explore how to bring interpretability to data-driven DSMD. Specifically, we propose a more interpretable decision process to implement the dialogue manager of DSMD by reasonably mimicking real doctors' inquiry logics, and we devise a model with highly transparent components to conduct the inference. Moreover, we collect a new DSMD dataset, which has a much larger scale, more diverse patterns, and is of higher quality than the existing ones. The experiments show that our method obtains 7.7%, 10.0%, 3.0% absolute improvement in diagnosis accuracy respectively on three datasets, demonstrating the effectiveness of its rational decision process and model design. Our codes and the GMD-12 dataset are available at https://github.com/lwgkzl/BR-Agent.
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Mojsilović, Zoran, Nikola Utvić, Đorđe Stanić, and Radenko Arsenijević. "Adaptations in relationship between morphological characteristics and selected motor skills at karatekas." In Antropološki i teoantropološki pogled na fizičke aktivnosti (10). University of Priština – Faculty of Sport and Physical Education in Leposavić, 2024. http://dx.doi.org/10.5937/atavpa24025m.

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The aim of the study was to determine the mutual contribution of morphological characteristics (body height and body mass) and selected motor skills (flexibility, agility, repetitive and explosive strength) to the changes that followed karate training. Karate training for two years was used to induce changes in characteristics and abilities. The research had a longitudinal design in which fourteen karate fighters (M = 7; F = 7) took part. The first measurement was carried out in September 2016 (BW = 159.11 ± 15.19; BW = 54.39 ± 15.53; age = 12.79 ± 2.72), while the second was carried out in September 2018 (BW = 165.2 ± 10.11; BW = 58.80 ± 15.42; year = 14.79 ± 2.72). The measured variables were: body height (TV), body mass (TM), sit and reach (SID), splits (ŠPG), running 4x10m (4x10m), running 4x8m (4x8m), lifting the trunk for 30 seconds (DT30sek), mixed pull-ups (MZG), squats (ČUČ), standing long jump (SUD) and standing triple jump (TRM), from which pre- to post- percentage changes were calculated (D). Pirson correlation was used to determine associations between D variables. Significant correlations were found between DTV and DŠPG (r = 0.794; p = 0.019), DTM and DŠPG (r = 0.776; p = 0.002), DSID and D4x8m (r = -0.628; p = 0.022), DŠPG and DTRM (r = 0.657; p = 0.015), DDT30sek and DMZG (r = 0.791; p = 0.002), DDT30sedž and DTRM (r = 0.535; p = 0.049), DSUD and DTRM (r = 0.581; p = 0.029). The results of this study can help in understanding the development of karate athletes through karate training.
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Zhang, Ce, and Azim Eskandarian. "A Comparative Analysis of Object Detection Algorithms in Naturalistic Driving Videos." In ASME 2021 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/imece2021-69975.

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Abstract Intelligent vehicle research has been rapidly developing. Object detection is one of the most critical study areas for intelligent vehicle’s driving safety. This paper conducts a comparative analysis for two popular real-time object detection algorithms: Yolo-v4 and deconvolutional single shot multibox detector (DSSD) under a naturalistic driving environment. An 80-classes COCO dataset trains each neural network at first, then fine-tuned by the BDD100k dataset. The detection results are compared by True/False Positive Results, Precision-Recall Curve, and average precision @ intersection of union 50 and average precision @ intersection of union 75 results. According to the analysis results, the Yolo-v4 outperforms the DSSD algorithm in bad weather, nighttime conditions, and small object detections. The Yolo-v4 and DSSD mean average precision for the BDD dataset is 22.63 and 11.86, respectively. The Yolo-v4 precision is 90.81% better than the DSSD algorithm, proving that the Yolo-v4 is a better fit for real-world driving environment studies.
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Aman, Mortada A., and Egemen K. Çetinkaya. "DSB-SEIS." In the 4th Workshop. New York, New York, USA: ACM Press, 2016. http://dx.doi.org/10.1145/2955193.2955208.

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Csallner, Christoph, and Yannis Smaragdakis. "DSD-Crasher." In the 2006 international symposium. New York, New York, USA: ACM Press, 2006. http://dx.doi.org/10.1145/1146238.1146267.

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Moraru, L., T. G. Keith, F. Dimofte, S. Cioc, N. Ene, and D. P. Fleming. "A Dynamic Analysis of a Dual Clearance Squeeze Film Damper." In STLE/ASME 2010 International Joint Tribology Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/ijtc2010-41139.

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Squeeze film dampers (SFD) are devices utilized to control the shafts of high-speed rotating machinery. A dual squeeze film damper (DSFD) consists of two squeeze film bearings that are separated by a sleeve, which is released when the rotor experiences abnormal operating conditions. In this part of our study of DSFD we analyze the case when both the inner and the outer oil films are active. We present computed and measured unbalance responses of a shaft supported in DSFD. The oil forces which are utilized in the calculation of the unbalance response are obtained from numerical solutions of the Reynolds equation. A finite-difference algorithm is utilized for solving the pressure equation within the calculation of the dynamic response of the shaft.
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Bouzerdoum, A., and R. B. Pinter. "Motion detection in nonlinear lateral inhibitory neural networks." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tuz12.

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Motion is an important component of vision for flying insects. Optomotor reactions serve to minimize involuntary deviations of trajectories and stabilize the flight course. Insect optomotor responses are based on directional movement detection. The Reichardt correlation detector, essentially based on linear time-invariant filtering, cannot account for the saturation and adaptation properties exhibited by insect responses to motion. In this paper a directionally selective movement detecting (DSMD) neural network architecture, inspired by invertebrate vision, is presented. It is structurally and, to second order, functionally equivalent to a Reichardt detector, but it operates on the nonlinear mechanism of shunting lateral inhibition. Responses, under various constraints, of this DSMD neural network to moving patterns such as edges, bars, and sinusoidal gratings are investigated and compared to those of insect DSMD interneurons.
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Reports on the topic "DsbD"

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Wilson, Thomas E., Avraham A. Levy, and Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, March 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and strand invasion. Important proteins in this pathway are Rad51, Rad52 and Rad54. We proposed to combine our respective expertise: on the US side, in the design of zincfinger nucleases (ZFNs) for the induction of DNA DSBs at any desired genomic locus and in the integration of DNA molecules via NHEJ; and on the Israeli side in the HR events, downstream of the DSB, that lead to homology search and strand invasion. We sought to test three major pathways of targeted DNA integration: (i) integration by NHEJ into DSBs induced at desired sites by specially designed ZFNs; (ii) integration into DSBs induced at desired sites combined with the use of Rad51, Rad52 and Rad54 proteins to maximize the chances for efficient and precise HR-mediated vector insertion; (iii) stimulation of HR by Rad51, Rad52 and Rad54 in the absence of DSB induction. We also proposed to study the formation of dsT-DNA molecules during the transformation of plant cells. dsT-DNA molecules are an important substrate for HR and NHEJ-mediatedGT, yet the mode of their formation from single stranded T-DNA molecules is still obscure. In addition we sought to develop a system for assembly of multi-transgene binary vectors by using ZFNs. The latter may facilitate the production of binary vectors that may be ready for genome editing in transgenic plants. ZFNs were proposed for the induction of DSBs in genomic targets, namely, the FtsH2 gene whose loss of function can easily be identified in somatic tissues as white sectors, and the Cruciferin locus whose targeting by a GFP or RFP reporter vectors can give rise to fluorescent seeds. ZFNs were also proposed for the induction of DSBs in artificial targets and for assembly of multi-gene vectors. We finally sought to address two important cell types in terms of relevance to plant transformation, namely GT of germinal (egg) cells by floral dipping, and GT in somatic cells by root and leave transformation. To be successful, we made use of novel optimized expression cassettes that enable coexpression of all of the genes of interest (ZFNs and Rad genes) in the right tissues (egg or root cells) at the right time, namely when the GT vector is delivered into the cells. Methods were proposed for investigating the complementation of T-strands to dsDNA molecules in living plant cells. During the course of this research, we (i) designed, assembled and tested, in vitro, a pair of new ZFNs capable of targeting the Cruciferin gene, (ii) produced transgenic plants which expresses for ZFN monomers for targeting of the FtsH2 gene. Expression of these enzymes is controlled by constitutive or heat shock induced promoters, (iii) produced a large population of transgenic Arabidopsis lines in which mutated mGUS gene was incorporated into different genomic locations, (iv) designed a system for egg-cell-specific expression of ZFNs and RAD genes and initiate GT experiments, (v) demonstrated that we can achieve NHEJ-mediated gene replacement in plant cells (vi) developed a system for ZFN and homing endonuclease-mediated assembly of multigene plant transformation vectors and (vii) explored the mechanism of dsTDNA formation in plant cells. This work has substantially advanced our understanding of the mechanisms of DNA integration into plants and furthered the development of important new tools for GT in plants.
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Parker, Robert F. Dual-Slab Verification Detector (DSVD) Operations Software User Guide. Office of Scientific and Technical Information (OSTI), June 2014. http://dx.doi.org/10.2172/1134774.

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Aslam, Tariq D. Detonation Shock Dynamics (DSD) Calibration for LX-17. Office of Scientific and Technical Information (OSTI), April 2012. http://dx.doi.org/10.2172/1039314.

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DEFENSE SCIENCE BOARD WASHINGTON DC. Defense Science Board Force Protection Panel Report to DSB. Fort Belvoir, VA: Defense Technical Information Center, December 1997. http://dx.doi.org/10.21236/ada432505.

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Aida, Toru. Effects of hydro mesh resolution on Detonation Shock Dynamics (DSD). Office of Scientific and Technical Information (OSTI), June 2013. http://dx.doi.org/10.2172/1083093.

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Aida, Toru. Effects of hydro mesh resolution on Detonation Shock Dynamics (DSD). Office of Scientific and Technical Information (OSTI), June 2013. http://dx.doi.org/10.2172/1083107.

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Aslam, T. D., J. B. Bdzil, and L. G. Hill. Extensions to DSD theory: Analysis of PBX 9502 rate stick data. Office of Scientific and Technical Information (OSTI), December 1998. http://dx.doi.org/10.2172/350869.

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Kaul, Ann. Notes on the ExactPack Implementation of the DSD Rate Stick Solver. Office of Scientific and Technical Information (OSTI), August 2016. http://dx.doi.org/10.2172/1291274.

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Kaul, Ann, and Scott William Doebling. Notes on the ExactPack Implementation of the DSD Explosive Arc Solver. Office of Scientific and Technical Information (OSTI), January 2017. http://dx.doi.org/10.2172/1340910.

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Hwang, Sheng-Kwang. Nonlinear Dynamics of Photonics for Optical Signal Processing - Optical Frequency Conversion and Optical DSB-to-SSB Conversion. Fort Belvoir, VA: Defense Technical Information Center, September 2015. http://dx.doi.org/10.21236/ada626951.

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