Relevant bibliographies by topics / Droplet Recombinase Polymerase Amplification

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  2. Dissertations / Theses
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Academic literature on the topic 'Droplet Recombinase Polymerase Amplification'

Author: Grafiati
Published: 6 September 2023
Last updated: 31 July 2025

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Journal articles on the topic "Droplet Recombinase Polymerase Amplification"

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Schuler, Friedrich, Frank Schwemmer, Martin Trotter, et al. "Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA." Lab on a Chip 15, no. 13 (2015): 2759–66. http://dx.doi.org/10.1039/c5lc00291e.

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Cui, Johnson Q., Frank X. Liu, Hojeong Park, et al. "Droplet digital recombinase polymerase amplification (ddRPA) reaction unlocking via picoinjection." Biosensors and Bioelectronics 202 (April 2022): 114019. http://dx.doi.org/10.1016/j.bios.2022.114019.

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Ibekwe, Mark A., Shelton E. Murinda, Stanley Park, et al. "Comparative Use of Quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and Recombinase Polymerase Amplification (RPA) in the Detection of Shiga Toxin-Producing E. coli (STEC) in Environmental Samples." Water 12, no. 12 (2020): 3507. http://dx.doi.org/10.3390/w12123507.

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E. coli O157:H7 is a foodborne pathogen that constitutes a global threat to human health. However, the quantification of this pathogen in food and environmental samples may be problematic at the low cell numbers commonly encountered in environmental samples. In this study, we used recombinase polymerase amplification (RPA) for the detection of E. coli O157:H7, real-time quantitative PCR (qPCR) for quantification, and droplet digital PCR (ddPCR) for absolute and accurate quantification of E. coli O157:H7 from spiked and environmental samples. Primer and probe sets were used for the detection of
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Lin, Hsing-Ying, Chen-Han Huang, Peng-Wei Hsu, et al. "(Invited) Aiot-Integrated Digital Imaging Sensor for Molecular-Fingerprint Profiling of Extracellular Vesicles in Liquid Biopsy." ECS Meeting Abstracts MA2024-01, no. 33 (2024): 1595. http://dx.doi.org/10.1149/ma2024-01331595mtgabs.

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The advancement of liquid biopsy techniques for non-invasive tumor analysis holds immense potential in continuously tracking tumor status—recurrence, progression, and treatment response—in real-time. To comprehensively assess tumor evolution, cellular diversity, and drug resistance mechanisms, a pivotal step involves scrutinizing proteins and nucleic acids within extracellular vesicles (EVs) present in biofluids. Our focus lies in crafting an advanced digital bead-based sensor system that seamlessly evaluates the molecular profile of EVs in glioblastoma cases. Our innovative approach enriches
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Lin, Hsing-Ying, Chen-Han Huang, Peng-Wei Hsu, et al. "(Invited) liquid Biopsy Tool: Integrated Aiot Digital Bead-Based Sensor for Molecular-Fingerprint Profiling of Extracellular Vesicles." ECS Meeting Abstracts MA2023-01, no. 34 (2023): 1914. http://dx.doi.org/10.1149/ma2023-01341914mtgabs.

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Development of liquid biopsies for non-invasive tumor characterization techniques shows great promise for real-time monitoring of tumor recurrence, progression, and treatment response. To better assess the various tumor evolution, cellular heterogeneity, consequent drug-resistance mechanisms, it is critical to screen proteins and nucleic acids of extracellular vesicles (EVs) in biofluids. We are developing an integrated sensitive digital bead-based sensor enabling evaluation of molecular profiling of extracellular vesicles in glioblastoma. The tumor relevant genetic information in EVs is enric
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Chowdhury, Rajashree, Prakash Ghosh, Md Anik Ashfaq Khan, et al. "Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis." Tropical Medicine and Infectious Disease 5, no. 2 (2020): 95. http://dx.doi.org/10.3390/tropicalmed5020095.

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To detect Post-kala-azar leishmaniasis (PKDL) cases, several molecular methods with promising diagnostic efficacy have been developed that involve complicated and expensive DNA extraction methods, thus limiting their application in resource-poor settings. As an alternative, we evaluated two rapid DNA extraction methods and determined their impact on the detection of the parasite DNA using our newly developed recombinase polymerase amplification (RPA) assay. Skin samples were collected from suspected PKDL cases following their diagnosis through national guidelines. The extracted DNA from three
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Daher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples." Clinical Chemistry 60, no. 4 (2014): 660–66. http://dx.doi.org/10.1373/clinchem.2013.213504.

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Abstract BACKGROUND Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity. METHODS We studied the clinical performance of recombinase polymerase amplification for the screening of group B streptococci in vaginal/anal samples from 50 pregnant women. We also compared
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Daher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Recombinase Polymerase Amplification for Diagnostic Applications." Clinical Chemistry 62, no. 7 (2016): 947–58. http://dx.doi.org/10.1373/clinchem.2015.245829.

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Abstract BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular tool in many diagnostic fields represents an affordable (approximately 4.3 USD per test), simple (few and easy hands-on steps), fast (results within 5–20 min), and sensitive (single target copy number detected) method for the identification of pathogens and the detection of single nucleotide polymorphisms in human cancers and genetica
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Tomar, Saurabh, Barbora Lavickova, and Carlotta Guiducci. "Recombinase polymerase amplification in minimally buffered conditions." Biosensors and Bioelectronics 198 (February 2022): 113802. http://dx.doi.org/10.1016/j.bios.2021.113802.

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Khmeleva, S. A., G. R. Kutdusova, I. F. Duskaev, et al. "DEOXYURIDINE TRIPHOSPHATES MODIFIED WITH AROMATIC GROUPS OF TYROSINE OR TRYPTOPHAN FOR DIRECT ELECTROCHEMICAL DETERMINATION OF DOUBLE-STRANDED DNA AMPLIFICATION PRODUCTS." http://eng.biomos.ru/conference/articles.htm 1, no. 19 (2021): 248–50. http://dx.doi.org/10.37747/2312-640x-2021-19-248-250.

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A set of deoxyuridine triphosphates modified with aromatic groups of tyrosine or tryptophan was studied as substrates for amplification (by polymerase chain reaction or recombinase polymerase amplification) and as carriers of an electroactive ‘label’ for direct electrochemical detection of double-stranded DNA.
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Dissertations / Theses on the topic "Droplet Recombinase Polymerase Amplification"

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Daher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.

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Cette thèse de doctorat porte dans l’ensemble une étude approfondie sur une technologie émergente pour l’amplification isotherme des acides nucléiques appelée recombinase polymerase amplification (RPA). L’introduction porte une description détaillée sur la RPA. Cette revue de littérature documente et discute les diverses applications de la RPA en soulignant les connaissances actuelles concernant les applications diagnostiques. Malgré la composition complexe de la RPA (6 à 7 protéines dans le même mélange réactionnel), cette dernière s’avère une technologie rapide (générant des résultats < 2
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Godow, Bratt Tora, Mathilda Stigenberg, Andreas Elenborg, Sarah Ågren, and Andreas Medhage. "To monitor the microbial biodiversity in soil within Uppsala." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-444210.

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This is an exploration of the potential for a citizen science project, with the goal to get the general public involved in microbial soil biodiversity around Uppsala, Sweden. Biodiversity serves an important role in how an ecosystem performs and functions. A large part of Earth's biodiversity exists below ground in soil, where microorganisms interact with plants. It would be beneficial to analyse the abundance and spread of some microorganisms in order to gain a better understanding of soil biodiversity. We suggest that one species family to study could be Phytophthora. Phytophthora is a genus
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Shahin, Khalid Elsayed Kamal Elsayed. "Development of control strategies for Francisella noatunensis subsp. orientalis in Nile tilapia, Oreochromis niloticus." Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28046.

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Nile tilapia, Oreochromis niloticus, is one of the most important farmed fish globally. One of the most serious bacterial diseases constraining global tilapia production is Francisellosis caused by Francisella noatunensis subsp. orientalis (Fno). Although outbreaks of Fno are increasing worldwide, there are no licenced commercial vaccines to prevent the disease for use on tilapia farms. Thus, the current treatment of choice is the use of antibiotics combined with increasing water temperature up to 30°C. Studies investigating the diversity of circulating Fno isolates and the immune response of
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Priyanka, V. "Droplet Isothermal Amplification For Nucleic Acid Quantification." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5643.

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Nucleic acid quantification (NAQ) is extensively employed for gene expression analysis, monitoring viral loads, detecting rare or dysfunctional cells, and assessing treatment regimes. The gold standard, quantitative polymerase chain reaction (qPCR), and the recent alternative, droplet digital PCR (ddPCR), provide accurate quantification of nucleic acids (NA). Albeit the requirement of thermal cycling and separate platforms for droplet generation, NA amplification, and signal detection, in the case of ddPCR increases the assay complexity and time, limiting its broad applicability. In this work
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Chou, Yu-Pao, and 周育葆. "Primer Design for Multiplex Polymerase Chain Reaction and Multiplex Isothermal Recombinase Polymerase Amplification." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/65p49j.

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碩士<br>國立交通大學<br>生物資訊及系統生物研究所<br>106<br>At present, most of the deoxyribonucleic acid (DNA) amplification techniques such as polymerase chain reaction (PCR). PCR relies on the thermal cycle machine, through denaturation, annealing, extension, the process requires precise control the temperature. Recombinase polymerase amplification (RPA) technology is developed by TwistDx in 2006. First, it makes the primer sequence and protein form a complex, the complex will find the location of the homologous sequence on the DNA template and open double-stranded DNA helix structure. Next, amplification was p
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Euler, Anna Milena. "Entwicklung von Rekombinase-Polymerase-Amplifikations-Verfahren zum schnellen Nachweis von hochpathogenen Erregern." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0022-603B-0.

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Ehnts, Kai Ilmo. "Entwicklung von Rekombinase-Polymerase-Amplifikations-Nachweisverfahren für virale Erreger von Atemwegsinfektionen." Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BAD4-F.

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Fechner, Kim. "Distribution of Mycobacterium avium subspecies paratuberculosis in clinically asymptomatic bulls and different non-ruminant species." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3EF4-9.

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Book chapters on the topic "Droplet Recombinase Polymerase Amplification"

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Bhat, Alangar Ishwara, and Govind Pratap Rao. "Recombinase Polymerase Amplification." In Springer Protocols Handbooks. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0334-5_40.

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Javaid, Lubna, Sumiah Wani, Nulevino Iralu, Shahjahan Rashid, Sahar Saleem, and Aflaq Hamid. "Recombinase Polymerase Amplification for Viral Detection." In Springer Protocols Handbooks. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4390-7_29.

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Vashi, Yoya, and Sachin Kumar. "Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases." In Springer Protocols Handbooks. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2043-4_17.

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Yang, F., Y. Su, F. G. Li, et al. "Rapid Virus Detection Using Recombinase Polymerase Amplification Assisted by Computational Amplicon-Complex Spectrum." In 12th Asian-Pacific Conference on Medical and Biological Engineering. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-51485-2_36.

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Glais, Laurent, and Emmanuel Jacquot. "Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays." In Plant Pathology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2620-6_16.

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Zhang, Yuhang, Jinqiang Hu, Qingmei Li, Junqing Guo, and Gaiping Zhang. "Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticks." In Methods in Microbiology. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mim.2019.11.008.

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Chaumpluk, P. "DIY Lab-on-a-chip Platforms: A Solution for Simple Nucleic Acid-based Assays in the Absence of Proper (Chip) Facilities." In Lab-on-a-chip Devices for Advanced Biomedicines. Royal Society of Chemistry, 2024. http://dx.doi.org/10.1039/9781837673476-00362.

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Lab-on-a-chip technology plays a key role in nucleic acid-based assays due to its ability to overcome many issues affecting testing methods that depend heavily on a laboratory setting, which can often be time-consuming and lack the flexibility to perform tests on-site. Yet, a classical lab-on-a-chip system also involves some technical difficulties, instead relating to design, platform used, and mechanical control. Since nucleic acid assay depends on nucleic acid amplification and nucleic acid detection, the main technical issues associated with these areas in the context of chip fabrication ar
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Sephalika, Somya, and Bikash Ranjan Sahu. "RECENT ADVANCES IN LATERAL FLOW ASSAY FOR DETECTION OF PLANT PATHOGENIC BACTERIA." In Futuristic Trends in Agriculture Engineering & Food Sciences Volume 3 Book 9. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3biag9p1ch1.

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Plant pathogenic bacteria (PPBs) are widespread and pose a significant threat to global agriculture due to their ability to cause several diseases. Early and accurate detection of these pathogens is crucial for effective plant protection and disease management. Conventional methods for detecting plant pathogenic bacteria including serological and molecular techniques are, undoubtly reliable; however, these techniques can be time-consuming to analyse results. To cure the bacteria borne disease in plants, there is a need for ‘on site’ detection in field to enable treatment protocol precisely. To
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Conference papers on the topic "Droplet Recombinase Polymerase Amplification"

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Chiu, Nan-Fu, Ying-Hao Wang, Ming-Jung Tai, and Tien-Hsiung Ku. "Development of SPR biosensors for quantitative detection of SARS-CoV-2 via recombinase polymerase amplification." In Optical Sensors 2025, edited by Robert A. Lieberman, Francesco Baldini, and Jiri Homola. SPIE, 2025. https://doi.org/10.1117/12.3056568.

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Zhou, Tianqi, Xiangyu Jin, Fan Yang, et al. "Label-free recombinase polymerase amplification with hyperspectral digital optofluidics." In Optics in Health Care and Biomedical Optics XIII, edited by Qingming Luo, Xingde Li, Ying Gu, and Dan Zhu. SPIE, 2023. http://dx.doi.org/10.1117/12.2684913.

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Kapitonova, M. A., A. V. Shabalina, V. G. Dedkov, and A. S. Dolgova. "DEVELOPMENT OF DIAGNOSTIC SYSTEM FOR MAMMARENAVIRUS JUNINENSE VIRUS DETECTION BASED ON ISOTHERMAL AMPLIFICATION RPA." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-241.

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Here we present the development and testing of two methods for Mammarenavirus juninense virus detection based on isothermal recombinase polymerase amplification (RPA). The first method is a combination in a single tube of RPA and the DETECTR based on a specific nuclease activity of Cas12a. The second method is a real-time RPA with reverse transcription. The detection limit of the second approach is less than 10 copies of RNA.
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"Test performance study validation of a recombinase polymerase amplification (RPA) diagnostic assay for Phytophthora ramorum." In Plant Health 2024. American Phytopathological Society, 2024. http://dx.doi.org/10.1094/aps-ph24-088.

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"Development of a recombinase polymerase amplification assay for detection of Botrytis cinerea in fruit, vegetable, and soil samples." In Plant Health 2024. American Phytopathological Society, 2024. http://dx.doi.org/10.1094/aps-ph24-084.

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"Development of a recombinase polymerase amplification (RPA) assay to detect Colletotrichum acutatum and C. gloeosporiodes in strawberry nurseries." In Plant Health 2024. American Phytopathological Society, 2024. http://dx.doi.org/10.1094/aps-ph24-086.

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Timkin, P. D., and A. A. Penzin. "An experimental approach for diagnosing cercosporosis using RPA+CRISPR/Cas12a." In II All-Russian (national) scientific conference with international participation "Russian Science, Innovation, Education". Krasnoyarsk Science and Technology City Hall, 2023. http://dx.doi.org/10.47813/rosnio-ii.2023.8.263-266.

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The article demonstrates the prospects for using a new experimental approach for the detection of the fungus Cercospora Sojina Hara. The main principle underlying the presented method is a combination of two technologies: RPA (Recombinase polymerase amplification) and CRISPR/Cas12a. RPA - is an alternative to classical PCR, with features in the form of a faster reaction rate and its passage under isothermal conditions. Using RPA technology will reduce amplification to 15-30 minutes. Amplified genomic DNA can be detected fluorescently labeled with CRISPR/Cas12a. The difficulties of this method
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Sirr, Noel, Doina Ciobanu, Ronan Grimes, and Mark Davies. "A Continuous Flow Polymerase Chain Reactor for DNA Expression Analysis." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96180.

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The polymerase chain reaction (PCR) has revolutionised molecular biology, and is at the forefront of many current efforts to document and understand human genetic diversity. Recent years has seen a move towards incorporating the PCR technique into a micro Total Analysis System (μTAS) thus exploiting its full potential. Micro scale PCR design offers the opportunity to integrate all functional steps of DNA expression analysis into a miniaturised device allowing for high throughput and reduced analysis times, reduced sample volume requirements and cost efficiency. Consequently, it is desirable to
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Gaiani, Greta, Mònica Campàs, Anna Toldrà, et al. "Recombinase Polymerase Amplification for <em>Gambierdiscus </em>and <em>Fukuyoa </em>detection: a step further in the ciguatera risk management." In 1st International Electronic Conference on Toxins. MDPI, 2021. http://dx.doi.org/10.3390/iect2021-09168.

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Sayers, Michael B., and Tara M. Dalton. "A Novel Contamination Free Two Temperature Continuous Flow Polymerase Chain Reactor." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43055.

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Polymerase Chain Reaction (PCR) is an enzymatic process that has dramatically advanced many fields of life sciences, where it is an indispensable tool in a burgeoning range of applications, including diagnostic medicine, molecular biology, forensics and food testing. Recent increased demand for extremely high throughput PCR systems has led to the development of miniaturised continuous flow microfluidic PCR devices, which may have extremely high throughput compared to standard commercial PCR thermal cyclers. A novel continuous flow microfluidic PCR device has been designed and fabricated, consi
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