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1

Oetjen, Janina, and Barbara Reinhold-Hurek. "Characterization of the DraT/DraG System for Posttranslational Regulation of Nitrogenase in the Endophytic Betaproteobacterium Azoarcus sp. Strain BH72." Journal of Bacteriology 191, no. 11 (April 3, 2009): 3726–35. http://dx.doi.org/10.1128/jb.01720-08.

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ABSTRACT DraT/DraG-mediated posttranslational regulation of the nitrogenase Fe protein by ADP-ribosylation has been described for a few diazotrophic bacteria belonging to the class Alphaproteobacteria. Here we present for the first time the DraT/DraG system of a betaproteobacterium, Azoarcus sp. strain BH72, a diazotrophic grass endophyte. Its genome harbors one draT ortholog and two physically unlinked genes coding for ADP-ribosylhydrolases. Northern blot analysis revealed cotranscription of draT with two genes encoding hypothetical proteins. Furthermore, draT and draG2 were expressed under all studied conditions, whereas draG1 expression was nitrogen regulated. By using Western blot analysis of deletion mutants and nitrogenase assays in vivo, we demonstrated that DraT is required for the nitrogenase Fe protein modification but not for the physiological inactivation of nitrogenase activity. A second mechanism responsible for nitrogenase inactivation must operate in this bacterium, which is independent of DraT. Fe protein demodification was dependent mainly on DraG1, corroborating the assumption from phylogenetic analysis that DraG2 might be mostly involved in processes other than the posttranslational regulation of nitrogenase. Nitrogenase in vivo reactivation was impaired in a draG1 mutant and a mutant lacking both draG alleles after anaerobiosis shifts and subsequent adjustment to microaerobic conditions, suggesting that modified dinitrogenase reductase was inactive. Our results demonstrate that the DraT/DraG system, despite some differences, is functionally conserved in diazotrophic proteobacteria.
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2

Grussenmeyer, William. "Draw and drag." ACM SIGACCESS Accessibility and Computing, no. 111 (January 29, 2015): 10–13. http://dx.doi.org/10.1145/2809904.2809907.

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3

Zalewska, Beata, Rafał Piątek, Katarzyna Bury, Alfred Samet, Bogdan Nowicki, Stella Nowicki, and Józef Kur. "A surface-exposed DraD protein of uropathogenic Escherichia coli bearing Dr fimbriae may be expressed and secreted independently from DraC usher and DraE adhesin." Microbiology 151, no. 7 (July 1, 2005): 2477–86. http://dx.doi.org/10.1099/mic.0.28083-0.

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The dra gene cluster, expressed by uropathogenic Escherichia coli strains, determines bacterial attachment and invasion. The Dr fimbrial structures formed at the bacterial cell surface are composed of DraE subunits. The Dr fimbriae-coding cluster contains six open reading frames – draA, draB, draC, draD, draP and draE – among which the draE gene encodes the structural fimbrial subunit DraE. Very little is known about E. coli surface expression of the draD gene product. The expression of DraD and its role in the biogenesis of Dr fimbriae were determined by constructing mutants in the dra operon and by immunoblot and immunofluorescence experiments. In this study, DraD was found to be a surface-exposed protein. The expression of DraD was independent of the DraC usher and DraE fimbrial subunits. Polymerization of DraE fimbrial subunits into fimbrial structures did not require expression of the DraD protein.
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4

Zhang, Yaoping, Edward L. Pohlmann, Cale M. Halbleib, Paul W. Ludden, and Gary P. Roberts. "Effect of PII and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System inKlebsiella pneumoniae." Journal of Bacteriology 183, no. 5 (March 1, 2001): 1610–20. http://dx.doi.org/10.1128/jb.183.5.1610-1620.2001.

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ABSTRACT Reversible ADP-ribosylation of dinitrogenase reductase, catalyzed by the dinitrogenase reductase ADP-ribosyl transferase–dinitrogenase reductase-activating glycohydrolase (DRAT-DRAG) regulatory system, has been characterized in Rhodospirillum rubrum and other nitrogen-fixing bacteria. To investigate the mechanisms for the regulation of DRAT and DRAG activities, we studied the heterologous expression of R. rubrum draTG in Klebsiella pneumoniae glnB and glnK mutants. In K. pneumoniae wild type, the regulation of both DRAT and DRAG activity appears to be comparable to that seen in R. rubrum. However, the regulation of both DRAT and DRAG activities is altered in a glnB background. Some DRAT escapes regulation and becomes active under N-limiting conditions. The regulation of DRAG activity is also altered in a glnBmutant, with DRAG being inactivated more slowly in response to NH4 + treatment than is seen in wild type, resulting in a high residual nitrogenase activity. In aglnK background, the regulation of DRAT activity is similar to that seen in wild type. However, the regulation of DRAG activity is completely abolished in the glnK mutant; DRAG remains active even after NH4 + addition, so there is no loss of nitrogenase activity. The results with this heterologous expression system have implications for DRAT-DRAG regulation inR. rubrum.
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5

Hovda, Remi Johansen. "Drag." DRAMA 59, no. 1 (June 9, 2022): 40–45. http://dx.doi.org/10.18261/drama.59.1.10.

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6

Wang, H., and A. Norén. "Metabolic regulation of nitrogen fixation in Rhodospirillum rubrum." Biochemical Society Transactions 34, no. 1 (January 20, 2006): 160–61. http://dx.doi.org/10.1042/bst0340160.

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Nitrogenase activity in Rhodospirillum rubrum is post-translationally regulated by DRAG (dinitrogenase reductase glycohydrolase) and DRAT (dinitrogenase reductase ADP-ribosylation transferase). When a sudden increase in fixed nitrogen concentration or energy depletion is sensed by the cells, DRAG is inactivated and DRAT activated. We propose that the regulation of DRAG is dependent on its location in the cell and the presence of an ammonium-sensing protein.
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7

Raupach, M. R. "Drag and drag partition on rough surfaces." Boundary-Layer Meteorology 60, no. 4 (September 1992): 375–95. http://dx.doi.org/10.1007/bf00155203.

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8

BRANDWOOD, A. "Mechanical Properties and Factors of Safety of Spider Drag-lines." Journal of Experimental Biology 116, no. 1 (May 1, 1985): 141–51. http://dx.doi.org/10.1242/jeb.116.1.141.

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Experiments were carried out to determine the strain energy capacity and breaking strain of spider drag-line silk. These properties are discussed in terms of a mathematical model of a spider falling on a drag-line. It was found that the strain energy capacity of the drag-line was in sufficient to absorb the potential energy lost by a falling spider and that in order to avoid failure of the drag-line the spider dissipates energy by other means, particularly by using itsinertia to draw drag-line silk from its spinnerets.
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9

Senra, Andrés. "Drag Attack." TSQ: Transgender Studies Quarterly 8, no. 4 (November 1, 2021): 548–49. http://dx.doi.org/10.1215/23289252-9311186.

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10

Fritzsche, Sonja. "Fascist drag." Science Fiction Film & Television 15, no. 1 (February 1, 2022): 21–39. http://dx.doi.org/10.3828/sfftv.2022.3.

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Timo Vuorensola’s Finnish-German sf satire Iron Sky (2012) tells the story of a Nazi invasion from the Moon. At the time, a Nazi return seemed to be far-fetched, even ridiculous. The pointed critique of a Sarah Palin-like president who wins a second term using fascist propaganda techniques was overshadowed by the film’s space opera setting. Although certainly not unique in its critique of the US, this Naziploitation parody was eerily prescient with regards to what Umberto Eco has termed an eternal fascism that will return again and again. Thus, the film invites further questions regarding what is a shift away from the one-sided representation of Nazis in popular sf film. Through a discussion of German scholar Katrin Sieg’s term “ethnic drag,” this article analyzes the intersections of the representation of race and fascism in the film. It posits the term “fascist drag” to refer to Nazi representation as well as a linguistic break in which discussions of fascism in the West became taboo during the Cold War. These movements have until recently remained buried in postwar memory and rehearsed in the popular imagination.
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11

Brown, J. Brian. "Doing drag." Visual Sociology 16, no. 1 (January 2001): 37–54. http://dx.doi.org/10.1080/14725860108583825.

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12

Nordstrom, Susan Naomi, and Alison Happel-Parkins. "Methodological Drag." Qualitative Inquiry 22, no. 2 (December 16, 2015): 149–53. http://dx.doi.org/10.1177/1077800415620221.

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13

Miguel Ariza, Luis. "Einstein's Drag." Scientific American 279, no. 1 (July 1998): 24–25. http://dx.doi.org/10.1038/scientificamerican0798-24.

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14

Zaborskis, Mary. "Age Drag." WSQ: Women's Studies Quarterly 43, no. 1-2 (2015): 115–29. http://dx.doi.org/10.1353/wsq.2015.0020.

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15

Patterson, Jennifer Lyn. "Capital Drag." Journal of Homosexuality 43, no. 3-4 (April 29, 2003): 99–123. http://dx.doi.org/10.1300/j082v43n03_07.

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16

Mallinckrodt, A. John. "Drag Forces." Physics Teacher 41, no. 5 (May 2003): 261–62. http://dx.doi.org/10.1119/1.1571255.

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17

Bonfim Júnior, Paulo Luiz Silva, and Luiz Felipe Zago. "Drag Gamer:." Culturas Midiáticas 13, no. 2 (December 17, 2020): 170–87. http://dx.doi.org/10.22478/ufpb.1983-5930.2020v13n2.55672.

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Este artigo aborda a aparição da drag Samira Close na plataforma Facebook Gaming. Samira Close é o nome da figura performada por Wenner Pereira, que joga games assumindo-se como drag. O objetivo do artigo é indicar as diferentes formas de interação entre ela e seus fãs, em suas transmissões ao vivo, focando especificamente nos modos como seu corpo aparece nas telas. Na comunicação produzida nas comunidades de fãs ocorre a formação de laços e o compartilhamento de interesses. Para o artigo, os conceitos empregados foram cibercultura, cultura gamer e cultura dos fãs. Na comunidade de fãs dessa jogadora em particular há características de proximidade e conexão dos indivíduos por meio dos interesses fortemente vinculados à estética da drag gamer Samira Close.
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18

Gu, Pin-Gao, Douglas N. C. Lin, and Ethan T. Vishniac. "Drag Instability." Astrophysics and Space Science 292, no. 1-4 (2004): 261–65. http://dx.doi.org/10.1023/b:astr.0000045025.13684.ae.

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19

Howlett, Rory. "Drag artist." Nature 373, no. 6510 (January 1995): 114. http://dx.doi.org/10.1038/373114a0.

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20

Goldmark, Matthew. "National Drag." GLQ: A Journal of Lesbian and Gay Studies 21, no. 4 (2015): 501–20. http://dx.doi.org/10.1215/10642684-3123665.

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21

Corey, Frederick C. "“Masculine drag”." Critical Studies in Media Communication 17, no. 1 (March 2000): 108–10. http://dx.doi.org/10.1080/15295030009388380.

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22

Stix, Gary. "Drag Race." Scientific American 266, no. 3 (March 1992): 107–8. http://dx.doi.org/10.1038/scientificamerican0392-107.

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23

Stevens, Adam D. F. "Drag Syndrome." Drama Therapy Review 10, no. 1 (April 1, 2024): 113–17. http://dx.doi.org/10.1386/dtr_00150_5.

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24

Bessen, Mark. "Drag Brunch." After Dinner Conversation 4, no. 11 (2023): 17–37. http://dx.doi.org/10.5840/adc2023411103.

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Are drags shows modern minstrel shows for straight party girls? Can defense of values be compromised for special occasions? In this work of philosophical short story fiction, Hannah is getting married and off to Miami for a girl’s weekend bachelorette party. Her longtime gay friend Kyle, is not invited. Hannah’s mother has budgeted $100,000 for the wedding and bachelorette party on the condition Kyle not be invited. Hannah’s wedding is her special day, the money will make it perfect, so she has her bridesmaid (who should have been Kyle!) message Kyle, last minute, to uninvite him. Of course, she supports, gay rights, but not at the cost of her special day. While in Miami the over-the-top bachelorette group goes to a gay night club, then for mimosa and a drag show the next morning to recover. Kyle tried to contact Hannah to talk to her about her reasoning, but she refuses to pick up the phone, so he flies to Miami and confronts her at the drag show about being a fair-weather liberal, in spectacular fashion.
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25

Heeps, Graham. "Drag Act." Electric and Hybrid Vehicle Technology International 2018, no. 2 (January 2019): 6–8. http://dx.doi.org/10.12968/s1467-5560(22)60407-x.

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26

Yakunin, Alexander F., Alexander S. Fedorov, Tatyana V. Laurinavichene, Vadim M. Glaser, Nikolay S. Egorov, Anatoly A. Tsygankov, Vladislav V. Zinchenko, and Patrick C. Hallenbeck. "Regulation of nitrogenase in the photosynthetic bacteriumRhodobacter sphaeroidescontainingdraTGandnifHDKgenes fromRhodobacter capsulatus." Canadian Journal of Microbiology 47, no. 3 (March 1, 2001): 206–12. http://dx.doi.org/10.1139/w00-144.

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The photosynthetic bacteria Rhodobacter capsulatus and Rhodospirillum rubrum regulate their nitrogenase activity by the reversible ADP-ribosylation of nitrogenase Fe-protein in response to ammonium addition or darkness. This regulation is mediated by two enzymes, dinitrogenase reductase ADP-ribosyl transferase (DRAT) and dinitrogenase reductase activating glycohydrolase (DRAG). Recently, we demonstrated that another photosynthetic bacterium, Rhodobacter sphaeroides, appears to have no draTG genes, and no evidence of Fe-protein ADP-ribosylation was found in this bacterium under a variety of growth and incubation conditions. Here we show that four different strains of Rba. sphaeroides are incapable of modifying Fe-protein, whereas four out of five Rba. capsulatus strains possess this ability. Introduction of Rba. capsulatus draTG and nifHDK (structural genes for nitrogenase proteins) into Rba. sphaeroides had no effect on in vivo nitrogenase activity and on nitrogenase switch-off by ammonium. However, transfer of draTG from Rba. capsulatus was sufficient to confer on Rba. sphaeroides the ability to reversibly modify the nitrogenase Fe-protein in response to either ammonium addition or darkness. These data suggest that Rba. sphaeroides, which lacks DRAT and DRAG, possesses all the elements necessary for the transduction of signals generated by ammonium or darkness to these proteins.Key words: nitrogenase regulation, nitrogenase modification, photosynthetic bacteria.
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27

Rupp, Leila J., Verta Taylor, and Eve Ilana Shapiro. "Drag Queens and Drag Kings: The Difference Gender Makes." Sexualities 13, no. 3 (June 2010): 275–94. http://dx.doi.org/10.1177/1363460709352725.

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28

Wijesekera, H. W., E. Jarosz, W. J. Teague, D. W. Wang, D. B. Fribance, J. N. Moum, and S. J. Warner. "Measurements of Form and Frictional Drags over a Rough Topographic Bank." Journal of Physical Oceanography 44, no. 9 (September 1, 2014): 2409–32. http://dx.doi.org/10.1175/jpo-d-13-0230.1.

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Abstract Pressure differences across topography generate a form drag that opposes the flow in the water column, and viscous and pressure forces acting on roughness elements of the topographic surface generate a frictional drag on the bottom. Form drag and bottom roughness lengths were estimated over the East Flower Garden Bank (EFGB) in the Gulf of Mexico by combining an array of bottom pressure measurements and profiles of velocity and turbulent kinetic dissipation rates. The EFGB is a coral bank about 6 km wide and 10 km long located at the shelf edge that rises from 100-m water depth to about 18 m below the sea surface. The average frictional drag coefficient over the entire bank was estimated as 0.006 using roughness lengths that ranged from 0.001 cm for relatively smooth portions of the bank to 1–10 cm for very rough portions over the corals. The measured form drag over the bank showed multiple time-scale variability. Diurnal tides and low-frequency motions with periods ranging from 4 to 17 days generated form drags of about 2000 N m−1 with average drag coefficients ranging between 0.03 and 0.22, which are a factor of 5–35 times larger than the average frictional drag coefficient. Both linear wave and quadratic drag laws have similarities with the observed form drag. The form drag is an important flow retardation mechanism even in the presence of the large frictional drag associated with coral reefs and requires parameterization.
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29

TUCKER, VANCE A. "Measuring Aerodynamic Interference Drag Between a Bird Body and the Mounting Strut of a drag Balance." Journal of Experimental Biology 154, no. 1 (November 1, 1990): 439–61. http://dx.doi.org/10.1242/jeb.154.1.439.

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1. The drag of a bird body mounted on the strut of a drag balance in a wind tunnel is more than the sum of the drags of the isolated strut and the isolated body. The strut changes the air flow around the body and generates additional drag, known as interference drag. This paper describes practical methods for measuring the drag of bird bodies: a strain-gauge drag balance, dimensions for struts made with machine or hand tools, and a procedure for correcting drag measurements for interference drag. 2. Interference drag can be measured by extrapolating a relationship between the drag of isolated struts with different crosssectional sizes and shapes and the drag of a body mounted on those struts. The interference length the length of an isolated strut that produces drag equal to the interference drag is a usefulquantity for predicting interference drag. 3. The relationship mentioned above is a straight line for a model peregrine falcon (Falco peregrinus L.) body mounted on smooth struts struts with convex cross-sectional shapes ranging from streamlined to circular. This finding simplifies the determination of interference drag in three ways: (i) the line can be found from measurements with just two struts a standard strut with low drag and a calibration strut with high drag; (ii) the two struts need not have the same shape for example, the standard strut can be changed to a calibration strut by attaching a spoiler without disturbing the body mounted on the strut and (iii) a single value of interference length (33.1mm) describes smooth struts with a range of shapes and sizes. These struts had drag coefficients between 0.33 and 0.91 at Reynolds numbers between 2100 and 10800. 4. The interference length of a strut supporting the actual falcon body with a feathered surface is not significantly different from that of the strut supporting the model body with a rigid surface. 5. As a hypothesis, interference length (hI, in metres) of a smooth strut varieswith the size of the body mounted on it: hI=0.0365m0.333 where m is the body mass (in kg) of the intact bird.
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30

Zhang, Xue Peng, Yong Hua Wang, and Lu Quan Ren. "Wind Tunnel Test for Drag Reduction of Airfoil Bionic Soft Surface." Applied Mechanics and Materials 461 (November 2013): 767–78. http://dx.doi.org/10.4028/www.scientific.net/amm.461.767.

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The soft surface of birds and aquatic organisms in the nature can effectively reduce the drag. Inspired by the fact,in this paper, an attempt is made to stick silicone rubber soft surface on the surfaces of NACA 4412 and NACA 6409 airfoils. The drags, lifts and lift-drag ratios of airfoils with soft and rigid surfaces in 5 different thickness were compared through wind tunnel test under the condition of α = 0 °. The results show that most of the bionic soft surfaces play the role of reducing the aerodynamic drag, and also increasing the lift at the same time, in which the soft surface of 0.6mm had the most significant effect of drag reduction and lift increasing.
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31

Klein, Jennie, and Laurence Senelick. "What a Drag!" PAJ: A Journal of Performance and Art 23, no. 3 (September 2001): 90. http://dx.doi.org/10.2307/3246338.

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32

Terasaki, Ichiro. "Drag Delivers Electricity." JPSJ News and Comments 16 (January 15, 2019): 11. http://dx.doi.org/10.7566/jpsjnc.16.11.

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33

Florio, Brendan James, Robert McKibbin, Calum Braham, John Ockendon, and Colin Please. "Estimating Transonic Drag." ANZIAM Journal 59 (June 20, 2019): 1. http://dx.doi.org/10.21914/anziamj.v59i0.13408.

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34

Stone, David R. "Drag Queen Storytimes." Journal of Intellectual Freedom & Privacy 4, no. 1 (June 3, 2019): 67. http://dx.doi.org/10.5860/jifp.v4i1.7014.

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EDITOR’S NOTE: Unlike many other forms of censorship, challenges to drag queen storytimes generally don’t target authors, titles, or content, but rather the method in which stories are presented to children. This compilation is by no means an exhaustive list of recent library events with drag queen readers, but a look at some that generated a significant level of controversy.
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Stone, David R. "Drag Queen Storytimes." Journal of Intellectual Freedom & Privacy 4, no. 2 (November 22, 2019): 65. http://dx.doi.org/10.5860/jifp.v4i2.7195.

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36

Stone, David R. "Drag Queen Storytimes." Journal of Intellectual Freedom & Privacy 4, no. 3 (April 10, 2020): 51. http://dx.doi.org/10.5860/jifp.v4i3.7327.

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37

Weihs, D., and J. Katz. "Transient induced drag." AIAA Journal 24, no. 7 (July 1986): 1203–5. http://dx.doi.org/10.2514/3.9418.

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38

Thomsen, Dietrick E. "Electrons with Drag." Science News 129, no. 19 (May 10, 1986): 298. http://dx.doi.org/10.2307/3970678.

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39

Pryor, Ashley. "Socrates in Drag." Epoché 14, no. 1 (2009): 77–93. http://dx.doi.org/10.5840/epoche200914123.

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40

Newcomb, William A. "Magnetohydrodynamic wave drag." Physics of Fluids B: Plasma Physics 3, no. 8 (August 1991): 1818–29. http://dx.doi.org/10.1063/1.859651.

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41

Matviyenko, Svitlana. "Cyberbody as drag." Digital Creativity 21, no. 1 (March 2010): 39–45. http://dx.doi.org/10.1080/14626261003654269.

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42

Paul, J. E. "Mental-fiscal drag." British Dental Journal 176, no. 3 (February 1994): 84–85. http://dx.doi.org/10.1038/sj.bdj.4808373.

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43

Usherwood, J. "WHAT A DRAG." Journal of Experimental Biology 213, no. 13 (June 11, 2010): vi. http://dx.doi.org/10.1242/jeb.036574.

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44

Rosenfeld, Kathryn. "Drag King Magic." Journal of Homosexuality 43, no. 3-4 (April 29, 2003): 201–19. http://dx.doi.org/10.1300/j082v43n03_13.

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45

Rhyne, Ragan. "Racializing White Drag." Journal of Homosexuality 46, no. 3-4 (April 20, 2004): 181–94. http://dx.doi.org/10.1300/j082v46n03_11.

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46

Brueningsen, Christopher, Jason Marinelli, Peter Pappano, and Kenneth Wallace. "Modeling air drag." Physics Teacher 32, no. 7 (October 1994): 439–41. http://dx.doi.org/10.1119/1.2344071.

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47

Bland, Stewart. "What a drag." Materials Today 14, no. 9 (September 2011): 378. http://dx.doi.org/10.1016/s1369-7021(11)70177-8.

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48

Galligan, J. M., T. J. McKrell, and M. T. Robson. "Dislocation drag processes." Materials Science and Engineering: A 287, no. 2 (August 2000): 259–64. http://dx.doi.org/10.1016/s0921-5093(00)00783-8.

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49

MacKenzie, P. M., and M. A. Forrester. "Sailboat propeller drag." Ocean Engineering 35, no. 1 (January 2008): 28–40. http://dx.doi.org/10.1016/j.oceaneng.2007.07.004.

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50

Hellsten, Martin. "Drag-reducing surfactants." Journal of Surfactants and Detergents 5, no. 1 (January 2002): 65–70. http://dx.doi.org/10.1007/s11743-002-0207-z.

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