Journal articles on the topic 'DPPH antioxidant activity'
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1
Karamać, M., A. Buciński, B. Pegg R, and R. Amarowicz. "Antioxidant and antiradical activity of ferulates." Czech Journal of Food Sciences 23, No. 2 (November 15, 2011): 64–68. http://dx.doi.org/10.17221/3373-cjfs.
Abstract:
Antioxidant and antiradical activities of ferulates (i.e., ferulic acid, isoferulic acid, coniferyl aldehyde, and methyl ferulate) were investigated using a β-carotene-linoleate model system and a DPPH radical scavenging assay, respectively. Compounds so tested exhibited antioxidant and antiradical properties to varying degrees. Methyl ferulate showed the strongest antioxidant activity, whereas the parent phenolic acid was the most active ferulate to scavenge the DPPH radical (DPPH<sup>·</sup>). Isoferulic acid at concentrations ranging from 10 to 100 nmol/assay did not impart an antiradical efficacy; this may be attributed to the location of the hydroxyl group in the meta position on the aromatic ring.
2
Gulcin, İlhami, and Saleh H. Alwasel. "DPPH Radical Scavenging Assay." Processes 11, no. 8 (July 26, 2023): 2248. http://dx.doi.org/10.3390/pr11082248.
Abstract:
Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals in human metabolism and their deterioration during processing and storage of fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, there has been a parallel increase in the use of assays to estimate antioxidant efficacy in human metabolism and food systems. Today, there are many bioanalytical methods that measure the antioxidant effect. Of these, the 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay is the most putative, popular, and commonly used method to determine antioxidant ability. In this review, a general approach to the DPPH radical scavenging assay has been taken. In this context, many studies, including attempts to adapt the DPPH radical scavenging method to different analytes, search for the highest antioxidant activity values, and optimize the method of measurement, have previously been performed. Therefore, it is highly important to introduce measures aimed at standardizing the conditions of the DPPH radical scavenging activity, including the various reaction media suitable for this assay. For this aim, the chemical and basic principles of DPPH free radical scavenging are defined and discussed in an outline. In addition, this study describes and defines the basic sections of DPPH free radical scavenging in food and biological systems. Additionally, some chemical, critical, and technical details of the DPPH free radical removal method are given. This is a simple assay in which the prospective compounds or herbal extracts are mixed with the DPPH solution and their absorbance is measured after a certain period. However, despite rapid advances in instrumental techniques and analysis, this method has not undergone extreme modification. This study presents detailed information about the DPPH method and an in-depth review of different developments.
3
Baliyan, Siddartha, Riya Mukherjee, Anjali Priyadarshini, Arpana Vibhuti, Archana Gupta, Ramendra Pati Pandey, and Chung-Ming Chang. "Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa." Molecules 27, no. 4 (February 16, 2022): 1326. http://dx.doi.org/10.3390/molecules27041326.
Abstract:
The use of F. religiosa might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in Ficus religiosa, as F. religiosa is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the measurement of antioxidant properties that includes the use of the free radicals used for assessing the potential of substances to serve as hydrogen providers or free-radical scavengers (FRS). The technique of DPPH testing is associated with the elimination of DPPH, which would be a stabilized free radical. The free-radical DPPH interacts with an odd electron to yield a strong absorbance at 517 nm, i.e., a purple hue. An FRS antioxidant, for example, reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen. It is radical in comparison to the DPPH-H form, because it causes decolorization, or a yellow hue, as the number of electrons absorbed increases. Decolorization affects the lowering capacity significantly. As soon as the DPPH solutions are combined with the hydrogen atom source, the lower state of diphenylpicrylhydrazine is formed, shedding its violet color. To explain the processes behind the DPPH tests, as well as their applicability to Ficus religiosa (F. religiosa) in the manufacture of metal oxide nanoparticles, in particular MgO, and their influence on antioxidants, a specimen from the test was chosen for further study. According to our findings, F. religiosa has antioxidant qualities and may be useful in the treatment of disorders caused by free radicals.
4
Yamauchi, Moeka, Yukino Kitamura, Haruka Nagano, Junya Kawatsu, and Hiroaki Gotoh. "DPPH Measurements and Structure—Activity Relationship Studies on the Antioxidant Capacity of Phenols." Antioxidants 13, no. 3 (March 1, 2024): 309. http://dx.doi.org/10.3390/antiox13030309.
Abstract:
The consumption of foods that are high in antioxidant capacity is believed to contribute to good health. Moreover, the addition of highly antioxidant compounds to foods is believed to prevent food deterioration. Among the known antioxidants in food, phenols have been identified as the primary antioxidants. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay is a simple, inexpensive, and rapid method widely used to evaluate the antioxidant capacity. Although the results of the DPPH assay depend on conditions such as the reaction time and concentration, the experimental conditions have not been standardized. Further, previous research that compared the antioxidant capacity determined through the DPPH assay largely focused on the differences in the specific substructures of approximately several dozen compounds. In this study, we conducted DPPH assays on 169 phenols under the same experimental conditions and summarized the correlation between their structures and activity. This DPPH assay study is the first single-laboratory investigation of the largest number of components in terms of their Trolox equivalent antioxidant capacities. Further, the analysis method was reproduced in an interlaboratory collaborative study, enabling its application in the reproduction and comparison of measurements in other laboratories.
5
Mandic, Anamarija, Sonja Djilas, Jasna Canadanovic-Brunet, Gordana Cetkovic, and Jelena Vulic. "Antioxidant activity of white grape seed extracts on DPPH radicals." Acta Periodica Technologica, no. 40 (2009): 53–61. http://dx.doi.org/10.2298/apt0940053m.
Abstract:
Composition and antioxidant activity of grape seed extract (GSE) obtained from red grape varietes are very well documented, in contrast to the white varietes. This paper presents the results of polyphenols content of ethyl acetate extract of grape seeds, obtained from two white grape varieties, Italian Riesling and Zupljanka, and their antioxidant activity on the stable DPPH radical. The influence of the addition of GSE to raspberry juice on the DPPH radical was also examined. Content of total polyphenols in GSEs ranged between 81.6 and 82.8% (w/w), and the contetn of flavan-3-ols between 66.2 and 91.0% (w/w). HPLC results showed that the most abundant components in the extract were (+)-catechin and (-)-epicatechin for both grape varieties. All tested GSEs exhibited good antioxidant activity. IC50 values for the GSEs of Italian Riesling and Zupljanka were 0.79 and 0.95 mg sample/mg DPPH radical, respectivelly. Since the GSE of Italian Riesling possesed stronger antioxidant activity, it was used for further experiments. The IC50 value for raspberry juice was 4.18 mg raspberry juice/mg DPPH. The raspberry juice with addition of 0.60 ?g/mL of GSE showed antioxidant activity of 39.2%. The same juice with the threefold concentration of vitamin C (1.81 ?g/ml) exhibited similar antioxidant activity (33.9%). Antioxidant activity of the same amount of juice without added antioxidants was lower (15.7%). The results showed that the GSE of white varietes could be considered as a good functional food ingredient.
6
Kumar, Mishra Arvind, Kumar S. Pawan, and Gupta Satish Chand. "ANTIBACTERIAL, ANTIFUNGAL AND DPPH RADICAL SCAVENGING ACTIVITY OF ACHYRANTHES ASPERA L." International Journal of Research in Ayurveda and Pharmacy 13, no. 2 (February 28, 2022): 17–20. http://dx.doi.org/10.7897/2277-4343.130228.
Abstract:
The plant Apamarg (Achyranthes aspera L.) was tested for antibacterial (Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus), antifungal (Aspergillus niger and Fusarium oxysporum) activities. For extraction, methanol extract was chosen. The DPPH Radical Scavenging activity was used to test the antioxidant action. The current study reveals a link between indigenous Achyranthes aspera L. treatment and in vitro antibacterial and antifungal responses. Ascorbic acid had the lowest DPPH scavenging activity than methanolic extracts of Achyranthes aspera L., which exhibited antioxidant activity. The effects of DPPH radical scavenging are dose-dependent. The stems and roots of Achyranthes aspera L. are high in antioxidants that could be utilized as a natural supplier of antioxidants and antimicrobial components.
7
Darweesh, Majida Jummaah. "Study antioxidant activity in Thalassemia Iraqi patients." Journal of Biotechnology Research Center 8, no. 4 (December 1, 2014): 39–41. http://dx.doi.org/10.24126/jobrc.2014.8.4.379.
Abstract:
The aims of this study was to estimate the total antioxidant activity level in serum among the Thalassemia Iraq patients. We estimated total free radical scavenging activity in the120 serum of Thalassemic patients their age range from 2-27years. The DPPH activity of serum ranged from 0.1-3.11µmol/ml, the mean serum level of total antioxidants in Thalassemia patients was significantly lower than the control group, and no relation to age or sex. We concluded that DPPH is useful for evaluating the total antioxidant capacity.
8
Triadisti, Nita, and Irfan Zamzani. "Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves." Borneo Journal of Pharmacy 6, no. 1 (February 28, 2023): 22–30. http://dx.doi.org/10.33084/bjop.v6i1.1830.
Abstract:
Available synthetic antioxidants have been reported to have mutagenic and toxic effects. On the other hand, natural antioxidants show their superiority as they are not or less toxic. Passiflora foetida has the potential as an antioxidant, but the investigation of the antioxidant activity of the P. foetida chromatography column fraction has not been reported. This studied aims to investigate the antioxidant activity of the column chromatographic fractions of P. foetida leaves. An antioxidant assay using the DPPH and FRAP methods. The extraction was carried out by graded maceration, then fractionation using column chromatography. The antioxidant activity test was carried out using the DPPH and FRAP methods. Thin Layer Chromatography analysis was performed to determine the chromatogram pattern. The EC50 using DPPH method from n-hexane extract: 129.035 µg/mL, ethyl acetate extract: 206.398 µg/mL, methanol extract: 97.453 µg/mL, while the EC50 using FRAP method from n-hexane extract: 67.851 µg/mL, ethyl acetate extract : 68.981 µg/mL, and methanol extract: 58.787 µg/mL. Column chromatography fractions have antioxidant activity, with FMetPF6 as the fraction with the best activity, with percent inhibition 41.85±1.96 at concentration 25 µg/mL (DPPH), and with percent antioxidant activity 26.03±0.84 at concentration 9 µg/mL (FRAP). Passiflora foetida leaves have great potential as an antioxidant; both the extract and its fractions have antioxidant activity. The FMetPF6 has the best activity compare to other extracts and fractions. Further analysis to determine the various compounds in FMetPF6 using LC-MS/MS will facilitate the active compound's isolation.
9
Angeli, Lucrezia, Sebastian Imperiale, Yubin Ding, Matteo Scampicchio, and Ksenia Morozova. "A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures." Antioxidants 10, no. 7 (June 24, 2021): 1019. http://dx.doi.org/10.3390/antiox10071019.
Abstract:
The 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay is widely used to determine the antioxidant activity of food products and extracts. However, the common DPPH• protocol uses a two-point measurement and does not give information about the kinetics of the reaction. A novel stoichio-kinetic model applied in this study monitors the consumption of DPPH• by common antioxidants following the second order reaction. The fitting of such decay yields the rate constant k1, which describes the main reaction between antioxidants and DPPH•, and the rate constant k2, which is attributed to a slower side reaction considering the products generated between the transient radicals (AO•) and another molecule of DPPH•. The model was first applied to antioxidant standards. Sinapic acid, Trolox and ascorbic and chlorogenic acids did not show any side reaction. Instead gallic, ferulic and caffeic acids achieved the best fitting with k2. The products of the side reaction for these compounds were confirmed and identified with high-resolution mass spectrometry. Finally, the kinetic model was applied to evaluate the antioxidant activity of eight herbal extracts. This study suggests a new kinetic approach to standardize the common DPPH• assay for the determination of antioxidant activity.
10
Sekti, Beta Herilla. "TEST OF ANTIOXIDANT CONTENT IN BLUE LOTUS (Nymphaea Stellata Wild) LEAVES WITH DPPH METHOD (1,1-Diphenyl-2-Picrylhydrazyl)." Jurnal Jamu Kusuma 1, no. 2 (December 31, 2021): 46–50. http://dx.doi.org/10.37341/jurnaljamukusuma.v1i2.10.
Abstract:
Antioxidants are compounds materials that can inhibit the rate of oxidation of other molecules or neutralize free radicals. Antioxidants can be obtained in synthetic or natural forms. Natural antioxidants can protect the human body from damage to active oxygen which can inhibit degenerative diseases and inhibit the production of fat and food. Natural antioxidants can be obtained from plants, one of which is the Blue Lotus Plant (Nymphaea Stellata Wild). To ensure the presence of antioxidants in this plant, the antioxidant activity was tested using the DPPH method. The DPPH method was used to determine the free radical scavenging activity of the Ethanol Extract of Blue Lotus Leaf (Nymphaea Stellata Wild). The positive control used was Ascorbic Acid. The IC50 value obtained for testing the antioxidant content of blue lotus leaf is 78.405 ppm ± 24.607. And Ascorbic Acid 2,238 ppm. It can be concluded that the antioxidant activity of Blue Lotus Leaf is not too different from the positive control of ascorbic acid. The antioxidant activity of blue lotus leaf was stated to be strong and ascorbic acid was stated to be very strong.
Keywords: Antioxidant, Free radicals, DPPH
11
Hartati, Rika, Hashifah I. Nadifan, and Irda Fidrianny. "Crystal Guava (Psidium guajava L. “Crystal”): Evaluation of In Vitro Antioxidant Capacities and Phytochemical Content." Scientific World Journal 2020 (September 1, 2020): 1–7. http://dx.doi.org/10.1155/2020/9413727.
Abstract:
Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural antioxidants is guava. The goals of this research were to investigate the antioxidant activity of leaves and fruit of crystal guava by determining the value of the Antioxidant Activity Index (AAI) using DPPH, CUPRAC, and FRAP; evaluate the total phenolic content (TPC) and total flavonoid content (TFC); analyse the correlation between the TPC and TFC with AAI DPPH, CUPRAC, and FRAP, and analyse the correlation between the 3 methods. Extraction was performed by the reflux method using n-hexane, ethyl acetate, and ethanol. Determination of AAI DPPH, CUPRAC, FRAP, the TPC, and the TFC was performed by UV-visible spectrophotometry. The correlation of the TPC and TFC with AAI DPPH, CUPRAC, and FRAP and, also, the correlation of the 3 methods were investigated by Pearson’s method. The antioxidant activity of leaves and fruit extracts of crystal guava showed AAI DPPH in the range of 0.33–56.46, CUPRAC 0.20–7.31, and FRAP 1.65–59.89. The highest TPC was given by ethanol leaf extracts (49.55 ± 1.45 g GAE/100 g), while the highest TFC was for n-hexane leaf extracts (9.68 ± 0.210 g QE/100 g). The TPC of leaves extract had a significantly positive correlation with AAI DPPH, CUPRAC, and FRAP. AAI DPPH, AAI CUPRAC, and AAI FRAP of leaves and fruit extract of crystal guava showed a significantly positive correlation. In general, leaves extract had strong antioxidant activity by the three methods. For the highest antioxidant activity, ethanol was the best solvent for extraction leaves and ethyl acetate for extraction fruit of crystal guava. The TPC in leaves extract contributed to the antioxidant activity by DPPH, CUPRAC, and FRAP methods. The Antioxidant activity of leaves and fruit extracts of crystal guava was linear by the three methods.
12
Ullah, Sana, and Chang-Gu Hyun. "Evaluation of Total Flavonoid, Total Phenolic Contents, and Antioxidant Activity of Strychnobiflavone." Indonesian Journal of Chemistry 20, no. 3 (May 9, 2020): 716. http://dx.doi.org/10.22146/ijc.44331.
Abstract:
This work evaluates the antioxidant activity of strychnobiflavone due to the increasing demand of the antioxidant agents day by day. Various in vitro antioxidants assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to investigate the antioxidant activity of strychnobiflavone. The results of both DPPH and ABTS show that strychnobiflavone increase the scavenging activity in a concentration-dependent manner due to the phenolic and flavonoid contents. This study revealed that strychnobiflavone is one of the promising and an effective compound for the antioxidant agent.
13
YILMAZ, Zehra Kübra, Belma ASIM, and Ozlem OZDEMIR. "In vitro antioxidant properties of 2-(4-(2-hydroxybenzylideneamino)benzylideneamino)benzoic acid." ICONTECH INTERNATIONAL JOURNAL 5, no. 3 (September 25, 2021): 7–12. http://dx.doi.org/10.46291/icontechvol5iss3pp7-12.
Abstract:
In recent years, it has attracted great attention to determine and appraise synthetic antioxidants with high free radical scavenging capacity associated with various disorders. Because synthetic antioxidants are widely used in place of native antioxidants as they are influential and less expensive. In this study, the antioxidant activity of the newly synthesized asymmetric diimine Schiff base was determined by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, ferrous ion chelation (FIC) activity and total antioxidant activity (Phosphomolybdenum assay) methods. The effective concentration (IC50) values were calculated for the Schiff base and standards. According to the results of DPPH radical scavenging, ferrous ion chelation and total antioxidant methods, Schiff base showed a well antioxidant activity. It was determined that Schiff base is reactive towards DPPH radicals (IC50, 180.0 μM) and especially Fe+2 ions (IC50, 76.3 μM). It was also found that it actively reduces the Mo(VI) ion to the Mo(V) form (IC50, 121.5 μM). The our results indicate Schiff base, may be a fine candidate as a source of synthetic antioxidants and possibly strong drug.
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Wijaya, Heri, Siti Jubaidah, and Siska Agustina. "Potential Antioxidant Activity of Kedondong Leaves (Spondias dulcis Forst.) Using DPPH Method (1,1-Diphenyl-2- Pikril Hydrazil)." International Journal of Advancement in Life Sciences Research 06, no. 02 (2023): 42–47. http://dx.doi.org/10.31632/ijalsr.2023.v06i02.005.
Abstract:
Background: Antioxidants are substances that can reduce free radicals to protect the body's biological systems from adverse effects arising from processes or reactions that cause excess oxidants. Kedondong leaves (Spondias dulcis Forst.) contain flavonoids, tannins, and alkaloids, which have the potential to act as antioxidants. Objective: To determine the antioxidant activity of ethyl acetate and 95% ethanol extracts from kedondong leaves. Methods: The antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, a free radical stable in an aqueous solution. Each extract was tested for its antioxidant activity with a comparison compound, vitamin C, using a UV-Vis spectrophotometer. The results of the antioxidant activity test revealed the IC50 (inhibitory concentration) value, namely the concentration of antioxidant compounds capable of inhibiting DPPH free radical activity by 50%. Result: The ethyl acetate extract has weak antioxidant activity with an IC50 value of 194.123 ppm, the 95% ethanol extract has very weak antioxidant activity with an IC50 value of 553.3694 ppm, and vitamin C, as a comparison, has very strong antioxidant activity with an IC50 value of 4.7805 ppm. Conclusion: Kedondong leaves have potential antioxidant activity but are very small.
15
Misrahanum, Misrahanum, Nurul Alfiyani, and Murniana Murniana. "Activity Antioxidant Ethanol Extract U Groh (Cocos nucifera L.) with DPPH Method." Journal of Pharmaceutical and Sciences 6, no. 2 (June 11, 2023): 388–92. http://dx.doi.org/10.36490/journal-jps.com.v6i2.98.
Abstract:
U groh shells and coir (Cocos nucifera L) have the potential as antioxidants. This study aimed to evaluate the IC50 value of young coconut ethanol extract as an antioxidant. The DPPH technique was used for extraction and antioxidant activity testing at concentrations of 6.25;12.5; 25; 50, and 100 ppm. The ethanol extracts of young coconut shell and coir generated antioxidant activity with IC50 values of 11.811 ppm and 42.483 ppm, respectively, and were classified as very active (50 ppm). As a result, young coconut shells and coir can be used as an antioxidant source.
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Hartati, Rika, Yasmine Widodo, Chrisanta Tarigan, and Irda Fidrianny. "Green Honey Deli Water Apple (Syzygium aqueum (Burm. f.) Alston “Madu Deli Hijau”): Evaluation of Antioxidant Activities and Phytochemical Content." Open Access Macedonian Journal of Medical Sciences 10, A (August 29, 2022): 1429–35. http://dx.doi.org/10.3889/oamjms.2022.9743.
Abstract:
BACKGROUND OF THE STUDY: Antioxidants are able to fight against free radicals which then prevent degenerative diseases. Antioxidants can be found in many plants such as water apples.
AIM OF THE STUDY: This research is aimed to determine the antioxidant activity of green honey deli water apple (Syzygium aqueum) leaves, branches, fruits extracts through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric Reducing Antioxidant Capacity (CUPRAC) methods, total phenolic content (TPC), total flavonoid content (TFC), correlation of TPC and TFC on antioxidant activity, correlation between DPPH and CUPRAC methods, and content of flavonoid compounds found in ethanol fruit extract of green honey deli water apple.
METHODOLOGY: Antioxidant activities were examined by determining ascorbic acid equivalent (AAE) through DPPH and CUPRAC methods. TPC and TFC were determined using UV-vis spectrophotometry. Correlation of TPC and TFC on antioxidant activity and correlation between DPPH and CUPRAC results were analyzed by Pearson’s method. Contents of flavonoid compounds were determined using HPLC.
RESULTS: Antioxidant activities of green honey deli water apple leaves, branches, and fruits extracts according to DPPH and CUPRAC methods were 3.97–354.96 mg AAE/g; 10.46–222.51 mg AAE/g respectively. Ethanol leaves extract had the highest TPC (68.14 ± 1.69 g GAE/100 g) and ethyl acetate leaves extract showed the highest TFC (18.65 ± g QE/100 g). TPC and TFC were found to correlate with the antioxidant activities. DPPH and CUPRAC results also correlated significantly positive.
CONCLUSION: Phenolic and flavonoid compounds had great contribution on antioxidant activities by DPPH and CUPRAC. The two methods exposed linear results. Ethanol fruits extract of green honey deli water apple contained quercetin (0.16%) and kaempferol (0.39%).
17
Tri Putri, Deby Kania, Beta Widya Oktiani, Candra Candra, and Rosihan Adhani. "ANTIOXIDANT ACTIVITY POTENCY OF CHITOSAN FROM HARUAN (CHANNA STRIATA) SCALES." Dentino : Jurnal Kedokteran Gigi 5, no. 2 (August 15, 2020): 139. http://dx.doi.org/10.20527/dentino.v5i2.8951.
Abstract:
ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.Keywords: Antioxidants, Chitosan, Haruan fish scalesABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity. Keywords: Antioxidants, Chitosan, Haruan fish scales.
18
Suhaj, M., and J. Rácová. "Study of irradiated black pepper antioxidant activity changes." Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (January 1, 2004): S187—S190. http://dx.doi.org/10.17221/10656-cjfs.
Abstract:
Some antioxidant activities of extracts of irradiated black pepper (Piper nigrum) were evaluated. The ground black pepper was exposed to gamma-irradiation at doses from 5 to 30 kGy. The effect of irradiation on antioxidant properties of black pepper extracts was investigated by these methods: radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, determination of reducing power, thiobarbituric acid reactive substances assay and electron paramagnetic resonance spectroscopy (EPR). Irradiation resulted in a decrease of DPPH antiradical activity of black pepper extracts according to dose of irradiation. Reducing power was not changed by action of irradiation. Very important and sensitive differences in antioxidant activity of irradiated black pepper were investigated by EPR spectrometry and thiobarbituric acid method.
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Wang, Xiang, Guang-Lei Zuo, Chao-Yue Wang, Hyun Yong Kim, Soon Sung Lim, and Sheng-Qiang Tong. "An Off-Line DPPH-GC-MS Coupling Countercurrent Chromatography Method for Screening, Identification, and Separation of Antioxidant Compounds in Essential Oil." Antioxidants 9, no. 8 (August 3, 2020): 702. http://dx.doi.org/10.3390/antiox9080702.
Abstract:
Essential oils are an important source of natural antioxidants and multiple methods have been established for evaluation of their overall antioxidant activity, however, the antioxidant activities of their compounds are less investigated. In the present study, the hyphenation of 2,2′-diphenyl-1-picrylhydrazyl (DPPH)-gas chromatography (GC)-mass spectrometry (MS) offline and high-speed countercurrent chromatography (HSCCC) is established for efficient screening, identification, and isolation of antioxidants from essential oils and applied to the essential oil of Curcuma wenyujin Y.H. Chen et C. Ling. Five compounds are preliminarily screened as antioxidants using DPPH-GC according to the reduction of GC peak areas of each compound after reaction with DPPH and then identified as eucalyptol (7.66%), camphor (2.34%), δ-elemene (1.15%), β-elemene (7.10%), and curzerene (15.77%) using GC-MS. Moreover, these five compounds are isolated by HSCCC using two solvent systems, n-hexane-acetonitrile-ethanol (5:3:2, v/v) and n-hexane-acetonitrile-acetone (4:3:1, v/v), and subjected to DPPH scavenging assay. Camphor, δ-elemene, and β-elemene show weak DPPH scavenging activity, while curzerene and eucalyptol show moderate DPPH scavenging activity. Notably, a significant synergistic effect on DPPH scavenging is found between curzerene and eucalyptol. The result demonstrated that off-line DPPH-GC-MS coupling CCC is an efficient method for screening, identification, and separation of antioxidant compounds in essential oil
20
Pujiastuti, Endra, and Rahma Sani Saputri. "Pengaruh Metode Pengeringan Terhadap Aktivitas Antioksidan Ekstrak Etanol." Cendekia Journal of Pharmacy 3, no. 1 (July 26, 2019): 44–52. http://dx.doi.org/10.31596/cjp.v3i1.43.
Abstract:
Drying is the most important step to keep the compound stability in simplicia and is very influential to produce good quality igredients in the antioxidant activity contained in the simplicia. Antioxidants are oxidation-free inhibitors of free radical reactions. Parijoto is one of the plants that have antioxidant compounds.This study aims to determine the effect of drying, concentration of antioxidant activity of parijoto (Medinilla speciosa Blume) fruit. The drying method used is direct sunlight drying, indirect sunlight and oven. The antioxidant activity of ethanol extract on drying method was determined by 2,2 diphenyl-1-picrylhidrazyl (DPPH) method which is free radical. The qualitative test DPPH showed that the higher levels of ethanol extract from the three drying methods further diminished the color of DPPH solution in ethanol.The result showed thet the drying method gave different effect to the antioxidant activity of parijoto fruit which is significant(p0,05). Drying methods wich have high antioxidant activity are given by drying using oven method with an IC50 value 33,75µg/ml.
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Mulyani, Elly, Herlina Herlina, Ijazati Alfitroh, Dewi Winni Fauziah, and Aina Fatkhil Haque. "Comparison of the Antioxidan Activity of the Ethanol Extract and Ethyl Acetate Fraction of Field Pavey (Nasturtium Montanum) Using the DPPH Method (1,1-diphenyl-2-2- picrylhydrazyl)." International Journal of Pharmaceutical Sciences and Medicine 8, no. 12 (December 30, 2023): 46–51. http://dx.doi.org/10.47760/ijpsm.2023.v08i12.005.
Abstract:
Antioxidants are substances that inhibit reactions by free radicals. One example of natural antioxidants is field mustard leaves. The use of field mustard leaves (Nasturtium montanum) in the manufacture of ethyl acetate fraction and its antioxidant activity is not yet known with certainty. Therefore, it is necessary to conduct research on the antioxidant activity of the ethyl acetate fraction of mustard greens (Nasturtium montanum) leaves.This research aims to determine the antioxidant activity in the ethyl acetate fraction of field mustard greens (Nasturtium montanum) using the DPPH (1,1-diphenyl-2-2-picrylhydrazyl) method. Ethyl acetate fraction samples were prepared with 4 concentration series of 40,60,80,100 ppm. Pipette 2 mL of each concentration and add 2 mL of 50 ppm DPPH. Then incubate for 30 minutes. Absorbance was measured using a UV-Vis spectrophotometer at λ 517 nm and expressed as an IC50 (Inhibitory Concentration) value which shows the concentration of an antioxidant compound that results in the capture of 50% of DPPH radicals. The results of the antioxidant activity test of the ethyl acetate fraction of field mustard greens (Nasturtium montanum) showed that there was antioxidant activity with an IC50 value of 98.57 µg/mL, classified as strong, 50-100 µg/mL. Based on this study, field mustard leaves (Nasturtium montanum) have strong antioxidant activity.
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Utami, Nova Elda, Salni Salni, and Marieska Verawaty. "ANTIOXIDANT ACTIVITY LEAVES KATIMAHA (Kleinhovia hospita L.)." BIOVALENTIA: Biological Research Journal 8, no. 1 (January 12, 2022): 22–31. http://dx.doi.org/10.24233/biov.8.1.2022.232.
Abstract:
The free radicals that people face in their daily activities require humans to get additional antioxidants from the outside. To overcome this problem, it is necessary to look for many sources of natural antioxidants considering the need for antioxidants that are increasingly needed Katimaha (K. hospita L.) is believed to have many benefits in the field of medicine. Therefore, katimaha has the potential to be used as a source of natural antioxidants. The research methods carried out were refining katimaha leaf simplicia, extraction, fractionation, antioxidant activity test with TLC plate, purification, determination of compound class, and antioxidant activity test using the DPPH method. The results showed that the active fraction of Katimaha leaf was the n-hexane fraction and the ethyl acetate fraction. Column chromatography on the active fraction found six pure eluates that had antioxidant activity, namely N1, N3, N5, E1, E2, and E4. Groups of compounds N1, N3, N5, E1 are terpenoids, group E2 eluates are tannins, while E3 eluates are flavonoid compounds. The pure eluate antioxidant activity test using the DPPH method obtained IC50 values of N1 are 40,142 ppm, N3 80,057 ppm, N5 271,643 ppm and E1 89,16 ppm, E2 124.91 ppm and E4 52.51 ppm.
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Taufiq, Nuramaniyah, and Sulfiani Sulfiani. "Antioxidant Activity of Ethanol and n-hexane Extracts of Javanese Bark (Lannea coromandelica) Using the DPPH Method." Indo. J. Chem. Res. 11, no. 1 (May 19, 2023): 43–48. http://dx.doi.org/10.30598//ijcr.2023.11-tau.
Abstract:
Javanese bark (Lannea coromandelica) contains secondary metabolites of saponins, tannins, phenolics, and flavonoids. Flavonoids include aromatic compounds that are antioxidants. Antioxidants can inhibit the oxidation process that arises due to free radical reactions to form unreactive compounds. The active flavonoid compounds in counteracting free radicals are determined by the presence of the –OH (hydroxy) functional group. Flavonoid compounds that have antioxidant properties include catechins, flavones, flavanones, flavonols, chalcones, and isoflavones. This study aims to determine the antioxidant activity of ethanol extract and n-hexane bark of Javan bark (Lannea coromandelica) using the DPPH method. The method used is DPPH using UV-VIS spectrophotometry. Ethanol extract has an IC50 of 3.996 mg/L and has a strong antioxidant activity while the antioxidant activity of N-Hexan is obtained IC50 2193.043 mg/L. has weak antioxidants weak antioxidants.
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Giamperi, Laura, Anahi Bucchini, Angela Bisio, Emanuela Giacomelli, Giovanni Romussi, and Donata Ricci. "Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates." Natural Product Communications 7, no. 2 (February 2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700221.
Abstract:
The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the extracts presented good antioxidant capacity.
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Ghosh, R., P. Barua, O. Sikder, S. Saha, S. Mojumder, and D. Sikdar. "Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities." Food Research 5, no. 6 (December 12, 2021): 187–96. http://dx.doi.org/10.26656/fr.2017.5(6).253.
Abstract:
Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.
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Ali Ahmad and Abdullahi Muhammad Abdu. "Evaluation of Toxicity and Antioxidant Activity of Hydro-Ethanol Leave Extract of Mentha piperita." Sahel Journal of Life Sciences FUDMA 1, no. 1 (December 31, 2023): 205–11. http://dx.doi.org/10.33003/sajols-2023-0101-022.
Abstract:
Mentha piperita has numerous pharmacological, cosmetic and alimental applications due to its ability to produce terpene and terpenoid compounds. Antioxidants are considered as important bioactive compound on account of many health benefits along with their vital role in delaying oxidative rancidity of numerous food. The study was to investigate acute toxicity evaluation and in vitro antioxidant activity of hydro-ethanol leave extracts of M. piperita. The acute toxicity was evaluate in rat according to (OECD). Animals were daily observed for 24 hours after treatment for mortality. The antioxidant activity of the extract was evaluated based on the method such as DPPH radical scavenging activity and reducing power method. The results showed that mortality was not observed in phase one but was observed in phase two. The LD50 by oral route in rat was less than 5000 mg/kg body weight. The percentage of antioxidant activity of leaf extracts of M. piperita was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, reducing power (RP) assay, ferric reducing/antioxidant power assay, and nitric oxide (NO) radical scavenging assay. The analysis revealed that NO scavenging activity is higher than DPPH with extract. NO and DPPH exhibited remarkable antioxidant activity. This indicates that the hydro-ethanol leave extract of M. piperita has a good antioxidant activity.
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Widowati, Wahyu, Teresa Liliana Wargasetia, Teddy Markus Zakaria, Meganita Marthania, Ria Aprilia Tri Puteri Permata Akbar, Michael Sebastian Gunadi, Nathanael Halim, and Sherly Santiadi. "Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea." Majalah Obat Tradisional 27, no. 1 (April 26, 2022): 32. http://dx.doi.org/10.22146/mot.71628.
Abstract:
Many people around the world believed that herbal tea had a lot of antioxidants contained in it, thus it was widely drunk all over the world. However, the antioxidant capacities of herbal tea may be various because it is affected by the usage of different plants as its primary ingredient. As a result, this study examines the antioxidant activity of herbal tea made from dried Citrus sp. and Clitoria ternatea flower petals. The antioxidant potential was assessed by performing a DPPH and H2O2 scavenging activity assay on 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay, total phenolic, and flavonoid content assay, ABTS reduction, and FRAP assay. This study showed that pure telang (Clitoria ternatea) herbal tea has antioxidant properties. Dried Telang (Clitoria ternatea) tea had the highest activity of antioxidants as we refer to the results of it had the highest activity value in DPPH, ABTS, and FRAP assay and also had the lowest IC-50 value. Followed by TEMON infusion which showed the second-highest antioxidant activity after pure telang infusion. The decrease in antioxidant activity in the mixture of telang and lemon was due to the mixture of lemons which tended to have the lowest antioxidant activity among the three types of infusions tested. The addition of dried lemon has the aim of enhancing the flavor of the tea infusion.
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Sawiji, Repining Tiyas, and Elisabeth Oriana Jawa La. "Uji Aktivitas Antioksidan Body Butter Ekstrak Etanol Kulit Buah Naga Merah dengan Metode DPPH." Jurnal Surya Medika 6, no. 2 (February 15, 2021): 178–84. http://dx.doi.org/10.33084/jsm.v6i2.2096.
Abstract:
Red dragon fruit peels (Hylocereus polyrhizus) are considered important by the community. While the red dragon fruit peel contains flavonoids, betacyanin, anthocyanin, and vitamin C which have antioxidant activity so that it can ward off free radiation and prevent cell damage. Antioxidants are molecules that can prevent the oxidation of other molecules. Antioxidants can protect the skin from various free radical cell damage. The body butter is a pharmaceutical preparation in the cosmetic field that can be used for skin health care. This study aims to determine the percentage of antioxidant activity and IC50 values in the body butter ethanol extract of red dragon fruit peels. Red dragon fruit peels were extracted first using the maceration method with 96% ethanol solvent. The antioxidant activity test was measured using UV-Vis spectrophotometry. The results showed that the antioxidant activity value of formula I obtained IC50 values (56.17 μg / mL), formula II IC50 values (88.26 μg / mL), and formula III IC50 values (146.05 μg / mL). From the results obtained, the body butter formula which has the greatest activity is the formula I (AAI = 0.7). Antioxidant activity in body butter preparations is included in the group of moderate antioxidants.
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Dewi, Ratna Sari, Desy Ayu Irma Permatasari, Tatiana Siska Wardani, and Muladi Putra Mahardika. "Antioxidant Activity Evaluation from Tomatoes’ N-Hexane, Ethyl Asetate, and Water Fraction with DPPH." Journal of Fundamental and Applied Pharmaceutical Science 2, no. 2 (March 7, 2022): 86–91. http://dx.doi.org/10.18196/jfaps.v2i2.13023.
Abstract:
Antioxidants are compounds that can stabilize free radicals in the body. Free radicals are highly reactive molecules as they have unpaired electrons to interact with body cell molecules. Tomatoes contain flavonoids, saponins, solanine tannins, folic acid, malic acid, citric acid, protein, fat, vitamins, minerals, and histamine, which can be used as antioxidants. This study aims to evaluate the antioxidant activity of n-hexane, ethyl acetate, water fraction, and ethanol extracts of Tomatoes and to determine the greatest antioxidant activity between n-hexane, ethyl acetate, water and vitamin C. Tomatoes (Lycopersicum esculentum Mill.) was extracted using the maceration method with ethanol followed by fractionation using n-hexane and ethyl acetate solvents. The test of antioxidant activity to DPPH radical was conducted on n-hexane, ethyl acetate, water, and vitamin C. The antioxidant activity results, expressed by IC50 value to the n-hexane, ethyl acetate, water fraction of Tomatoes fruit, were 4.4603 ppm; 4.0868 ppm; and 4.0527 ppm, respectively. Thus, the greatest antioxidant activity was the water fraction.
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Wang, Jing Chun, and Bao Qing Wang. "Antioxidant Activity of Schisandra Chinensis Extract and Derivatives." Advanced Materials Research 610-613 (December 2012): 3549–51. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.3549.
Abstract:
Antioxidant activities of acetone and methyl ethyl ketone extracts from leaves of Schisandra chinensis, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, methyl ethyl ketone extracts and derivative were superior to that of control with IC50 were 256.54, 342.28 and 124.32μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 278.29, 384.12 and 145.25μg/mL. It was concluded that Schisandra chinensis and its derivatives might be a potential natural source of antioxidants.
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Hadi, Ismanurrahman, and Teguh Adiyas Putra. "ANTIOXIDANT ACTIVITY OF ROASTED KEDAWUNG SEED (Parkia timoriana) USING SCAVENGER FREE RADICAL DPPH METHOD." Medical Sains : Jurnal Ilmiah Kefarmasian 8, no. 4 (December 31, 2023): 1655–60. http://dx.doi.org/10.37874/ms.v8i4.932.
Abstract:
Kedawung seeds (Parkia timoriana) contain polyphenolics and flavonoids that can provide pharmacological activities, such as antioxidants. In several regions of Indonesia, kedawung seeds are preserved using the roasting method. This study aimed to analyze the effect of roasting on the content of phytochemical compounds and determine their antioxidant activity using the DPPH method. Roasted kedawung seeds were extracted using a maceration method in ethanol. Phytochemical screening was performed to qualitatively analyze the contents of several phytochemical compounds in the extract. The results showed that roasted kedawung seed extract contained alkaloids, flavonoids, saponins, and terpenoids. Antioxidant testing was performed using the DPPH method with ascorbic acid as a positive control. The IC50 value of ascorbic acid, P. timoriana seed and roasted seed of P. timoriana measured each 12.3 ppm; 20.6 ppm and 9.8 ppm; which categorized as strong antioxidant compounds. These results indicated an increase in the antioxidant effect after roasted seed processing. It concluded that the roasting process could enhance antioxidant effect of P. timoriana seed Keywords: Kedawung seeds, roasted method, antioxidant, DPPH
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Onthoni, Mikhael Versen, Adithya Yudistira, and Deby Afriani Mpila. "ANTIOXIDANT ACTIVITY TEST OF ETANOL EXTRACT OF ALGA (Halimeda opuntia) TAKEN FROM THE SHORE OF POOPOH VILLAGE, MINAHASA DISTRICT." PHARMACON 12, no. 3 (August 24, 2023): 320–25. http://dx.doi.org/10.35799/pha.12.2023.49272.
Abstract:
Antioxidants are compounds that can protect body cells from damage caused by free radicals. Free radicals are unstable molecules that can damage body cells and cause oxidative stress. Antioxidants work by neutralizing free radicals to protect cells from damage and help maintain overall health. This study aims to test the antioxidant potential of ethanol extract of Halimeda opuntia algae taken from the southern coast of Minahasa Regency, North Sulawesi. Antioxidant activity was tested using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The decrease in absorbance value observed directly illustrates the decrease in the number of DPPH radicals remaining after the reaction between the extract and DPPH. The test results showed antioxidant levels that occurred at concentrations of 25 ppm (52.925%), 50 ppm (61.659%), 75 ppm (65.304%), and 100 ppm (70.112%). The test results show that the higher the concentration of ethanol extract of Halimeda opuntia algae, the stronger its ability to inhibit DPPH radicals. Therefore, it can be concluded that the highest antioxidant content is found at a concentration of 100 ppm with an average percent inhibition of 70.112%.
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Singh, Shalini, and Shivendra Verma. "DPPH Screening in Mentha and Guggul Plant Different Extract." International Journal of Agriculture and Animal Production, no. 33 (May 6, 2023): 33–38. http://dx.doi.org/10.55529//ijaap.33.33.38.
Abstract:
Free radicals are extremely reactive atoms or molecules that have one or more unpaired electrons on their outer orbital and have the potential to harm biological cells. The human body naturally creates antioxidants to combat free radicals, but because oxidative stress and aging cause more free radicals to develop, an exogenous antioxidant is required. The Plantae family has a lot of potential as a source of exogenous antioxidants because it is the largest family in the plant world. The goal of this study was to ascertain the antioxidant properties of the aqueous and ethanol extracts of mentha and guggul leaves, respectively. The DPPH technique was used to measure antioxidant activity, and the findings were noted for further study.
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Singh, Shalini, and Shivendra Verma. "DPPH Screening in Mentha and Guggul Plant Different Extract." International Journal of Agriculture and Animal Production, no. 33 (May 6, 2023): 33–37. http://dx.doi.org/10.55529/ijaap.33.33.37.
Abstract:
Free radicals are extremely reactive atoms or molecules that have one or more unpaired electrons on their outer orbital and have the potential to harm biological cells. The human body naturally creates antioxidants to combat free radicals, but because oxidative stress and aging cause more free radicals to develop, an exogenous antioxidant is required. The Plantae family has a lot of potential as a source of exogenous antioxidants because it is the largest family in the plant world. The goal of this study was to ascertain the antioxidant properties of the aqueous and ethanol extracts of mentha and guggul leaves, respectively. The DPPH technique was used to measure antioxidant activity, and the findings were noted for further study.
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Shen, Yanqiu, Jiayu Zhao, Guihong Zhou, Hui Xia, and Dong Liang. "Antioxidant Capacity Assessment of Two White Table Grape." E3S Web of Conferences 145 (2020): 01006. http://dx.doi.org/10.1051/e3sconf/202014501006.
Abstract:
Grapes can be an abundant source for polyphenols. This work was to extend the investigation to white grape antioxidants by determining antioxidants and antioxidant activity (DPPH, ABTS and FRAP). The results suggested the polyphenols of ‘Italia’ was significantly higher than ‘Rosario Bianco’. But except FRAP, DPPH and ABTS were no difference between two withe cultivars. Moreover, the seeds of tested grape cultivars were the best source of antioxidants content and activity, followed by skins and then pulps of grapes.
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Atila, Funda. "COMPARATIVE EVALUATION OF THE ANTIOXIDANT POTENTIAL OF Hericium erinaceus, Hericium americanum AND Hericium coralloides." Acta Scientiarum Polonorum Hortorum Cultus 18, no. 6 (December 17, 2019): 97–105. http://dx.doi.org/10.24326/asphc.2019.6.10.
Abstract:
The aim of this work was to determine the total phenolic content (TPC) and the antioxidant activity of methanol extracts of Hericium erinaceus, Hericium americanum and Hericium coralloides, including free radical scavenging method (DPPH), ferric reducing antioxidant power (FRAP) and radical cation scavenging method (ABTS). Hericium spp exhibited moderate to high antioxidant activity. The highest TPC (3.27 ±0.01 mg GAE g–1) and antioxidant activity values (17.0 ±0.68 mmol TE g‒1 in FRAP; EC50 = 4.12 ±0.12 mg mL‒1 in DPPH•; EC50 = 2.83 ± 0.10 mg mL‒1 in ABTS•+) were found for methanol extracts of H. coralloides. The TPC and antioxidant activity of H. erinaceus isolates varied from strain to strain. H. americanum possessed considerably lower total phenolic content (2.31 ±0.01 mg GAE g‒1) and antioxidant activity (10.5 ±0.59 mmol TE g‒1 in FRAP; EC50 = 7.82 ±0.09 mg mL‒1 in DPPH•; EC50 = 6.36 ± 0.12 mg mL‒1 in ABTS•+) than H. coralloides and H. erinaceus. A high correlation was determined between TPC and ABTS•+ (r2 = 0.855), DPPH• (r2 = 0.969) and FRAP (r2 = 0.942). According to results obtained in the present study, Hericium spp., especially H. coralloides and some of H. erinaceus isolates, might be promising natural source of antioxidants for food and pharmaceutical industry.
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Bhernama, Bhayu Gita, Witri Maulidy Ayu, and Cut Nuzlia. "Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)." JURNAL SAINS NATURAL 11, no. 2 (July 29, 2021): 79. http://dx.doi.org/10.31938/jsn.v11i2.309.
Abstract:
Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH
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Hasanah, Ulfatun, and Nurul Inayah. "Aktivitas Aantioksidan Kombinasi Daun Sirsak (Anonna Muricata L.) dan Daun Beluntas (Pluchea Indica L.)." ALCHEMY:Journal of Chemistry 11, no. 2 (October 3, 2023): 19–24. http://dx.doi.org/10.18860/al.v11i2.20183.
Abstract:
Soursop (Anonna Muricata L.) and Beluntas (Pluchea indica L.) are plants that contain secondary metabolites that have potential as antioxidants. The purpose of this study was to determine the activity of soursop leaf samples, beluntas and their combination in reducing DPPH. Samples were made into powder followed by maceration and evaporated the solvent to become an extract. Antioxidant activity testing with DPPH silencing method with UV-Vis spectrophotometer. Antioxidant activity test results for soursop leaf samples, beluntas, combinations (1:1), (1:2) and (2:1) have IC50 values of 58, 51, 57, 61 and 68 ppm, respectively. Further analysis Combination index of the combination (1:1) has a synergistic effect and the combination (1:2) and (2:1) has an antagonistic effect. Keywords: Antioxidant Activity, Anonna Muricata L. leaf, Pluchea indica L. leaf, DPPH
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Tahir, Karlina Amir, Upik A. Miskad, Khairuddin Djawad, Sartini Djide, Khaerani Khaerani, and Maulita Indrisari. "Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods." Open Access Macedonian Journal of Medical Sciences 9, A (April 22, 2021): 183–88. http://dx.doi.org/10.3889/oamjms.2021.5982.
Abstract:
BACKGROUND: Antioxidants are substances that can inhibit the oxidation process, so that they have a protective effect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto-Botto leaves (Chromolaena odorata L.). AIM: This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods. METHODS: The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods. RESULTS: The n-butanol fraction of Botto-Botto leaves had the highest antioxidant activity, namely the strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 μg / mL and 60.885 μg / mL, respectively. CONCLUSION: The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction.
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Chandra, Priyanka, and Daljit Singh Arora. "Antioxidant activity of fungi isolated from soil of different areas of Punjab,India." Journal of Applied and Natural Science 1, no. 2 (December 1, 2009): 123–28. http://dx.doi.org/10.31018/jans.v1i2.49.
Abstract:
The study was carried outto investigate the antioxidant activity of fungi isolated from soil of different areas of Punjab, India and compare their efficiency with a known antioxidant, ascorbic acid. The antioxidant potential of fungal extracts was quantified by DPPH and reducing power assay. Total phenolic contents were estimated using Folins-Ciocalteau (FC) reagent. Out of 113 fungal isolates selected, 51 were having antioxidant potential and these were further assayed quantitatively. All of these showed good activity against DPPH radical while 32 of these isolates demonstrated reducing potential also. In addition, some were equally good as ascorbic acid. The present study demonstrated potential of soil fungi to have antioxidant activity similar to plants and mushrooms. High phenolic content of fungi further highlight their significance as new sources of natural antioxidants. These fungi may provide easier set up for production and purification of natural antioxidants as compared to higher plants.
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Milardovic, Stjepan, Damir Iveković, Vlatko Rumenjak, and Božidar S Grabarić. "Use of DPPH⋅|DPPH Redox Couple for Biamperometric Determination of Antioxidant Activity." Electroanalysis 17, no. 20 (October 2005): 1847–53. http://dx.doi.org/10.1002/elan.200503312.
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Ikhrar, Muhammad S., Adithya Yudistira, and Defny S. Wewengkang. "UJI AKTIVITAS ANTIOKSIDAN Stylissa sp. DENGAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)." PHARMACON 8, no. 4 (November 28, 2019): 961. http://dx.doi.org/10.35799/pha.8.2019.29376.
Abstract:
ABSTRACTThis study aims to analyze the antioxidant activity of Stylissa sp., sponge. The Stylissa sp., sponge sample was obtained from the Lembeh Strait, waters Bitung. This research is an experimental laboratory by testing the ethanol extracts of Stylissa sp., sponge using DPPH method (1,1-diphenil2-pikrihidrazil) to analyze antioxidant activity using a spectrophotometer. The results of this study show the antioxidant levels of the Stylissa sp., sponge in the Lembeh Strait waters have antioxidant activity and the higher the concentration the higher the antioxidant levels produced. The greatest antioxidant level is found in Stylissa sp., sponge with a concentration of 100 mg / L. Keywords : Antioxidants, DPPH, Sponge Stylissa sp ABSTRAKPenelitian ini bertujuan untuk menganalisis aktivitas antioksidan dari Stylissa sp. Sampel Spons Stylissa sp di peroleh dari perairan Selat Lembeh, Bitung. Penelitian ini merupakan eksperimental laboratorium dengan pengujian terhadap ektrak etanol Spons Stylissa sp dengan metode DPPH (1,1-diphenil-2-pikrihidrazil) untuk menganalisis aktivitas antioksidan dengan menggunakan sprektrofotometer. Hasil penelitian ini memperlihatkan kadar antioksidan dari Spons Stylissa sp di perairan Selat Lembeh mempunyai aktivitas antioksidan dan semakin tinggi konsenstrasi semakin tinggi pula kadar antioksidan yang dihasilkan. Kadar antioksidan yang paling besar terdapat pada Spons Stylissa sp dengan konsentrasi 100 mg/L.Kata kunci : Antioksidan, DPPH, Spons Stylissa sp
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Halimah, Nur, Mahfuzun Bone, and Fajar Prasetya. "Uji Antioksidan Ekstrak Metanol Kulit Batang Pule (Alstonia scholaris) khas Kalimantan dengan Metode DPPH." Proceeding of Mulawarman Pharmaceuticals Conferences 14 (December 31, 2021): 90–95. http://dx.doi.org/10.25026/mpc.v14i1.576.
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Pule (Alstonia scholaris) is one of the plants that are empirically often used in medicine. Pule bark contains flavonoids which can generally be used as antioxidants. This study aims to determine the content of secondary metabolites and antioxidant activity of the methanol extract of pule bark by determining the IC50 value of the extract. The secondary metabolite test was carried out by reacting the extract with phenolic test reagents, steroids, terpenoids, saponins, flavonoids and alkaloids. The antioxidant activity test was carried out using the DPPH method (2,2-Diphenyl-1-Picrylhydrazil). Antioxidant activity was measured by the ability of the methanol extract of Pule bark to reduce DPPH which was measured using a UV-Vis Spectrophotometer so that absorbance data was obtained and then processed by linear regression method. The results showed that the extract contained phenolic and flavonoid compounds. While the DPPH test showed that the extract had weak antioxidant activity with an IC50 value of 472.473 ppm.
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Brudzyńska, Patrycja, Marzanna Kurzawa, Alina Sionkowska, and Michel Grisel. "Antioxidant Activity of Plant-Derived Colorants for Potential Cosmetic Application." Cosmetics 9, no. 4 (August 2, 2022): 81. http://dx.doi.org/10.3390/cosmetics9040081.
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Application of plant-derived colorants in products, i.e., cosmetics or food, apart from imparting the desired color without harming the environment, may provide other benefits. Valuable ingredients in cosmetic formulations include antioxidants showing an advantageous effect on the skin by neutralizing free radicals that accelerate the aging process and cause skin defects. Antioxidant activity can be determined by chemical-based methods. The aim of this study was to determine the antioxidant activity of plant-derived colorants (purple and red colorant) by two methods: CUPRAC and DPPH free-radical scavenging activity. Antioxidant activity evaluation using both methods for colorants samples was also performed after 5, 15, 30, and 60 min of exposure to UVC irradiation. The results obtained by CUPRAC method were for purple and red colorant unexposed samples as follows: 6.87 ± 0.09 and 4.48 ± 0.14 mg/100 mg colorant expressed as caffeic acid equivalent, respectively. UVC treatment did not affect the results of the antioxidant activity for red colorant and for the purple one only a slight influence was observed. DPPH free-radical scavenging activity for unexposed samples was 70.06 ± 7.74% DPPH/100 mg colorant for the red colorant and 96.11 ± 3.80% DPPH/100 mg colorant for the purple one.
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Fantoni, Muhammad, Indah Yulia Ningsih, Moch Amrun Hidayat, and Bambang Kuswandi. "Pengembangan Sensor Antioksidan berbasis Kertas Zonamikro dengan Imobilisasi DPPH pada Sampel Ekstrak Tanaman." Pustaka Kesehatan 11, no. 2 (May 8, 2023): 70. http://dx.doi.org/10.19184/pk.v11i2.12958.
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Development of a paper-based antioxidant sensor using DPPH reagent. This sensor is made using a screen-printing method to create a 5 mm diameter detection zone which is immobilized with DPPH reagents. The analysis was carried out in one step by immobilizing antioxidants/samples in the detection zone. After reduction by antioxidants, DPPH radicals become stable DPPH molecules, resulting in a change in color from purple to pale yellow. The purple intensity of DPPH was inversely proportional to the antioxidant activity of the sample and was measured using the help of ImageJ software. The optimal conditions for using DPPH reagents at a concentration of 5 mM and the volume in each detection zone were 3 µL. Characterization of this sensor analysis was carried out on gallic acid with the response time in the 12-minute, linearity with r = 0,9895, detection limit (LOD) value 0,0349 mM GAE, the quantitation limit (LOQ) value was 0,1164 mM GAE, with precision <2% (RSD), and meets the accuracy range of 97-103%. This sensor is then validated against DPPH spectrophotometry UV-Vis by analyzing antioxidant activity from plant extracts. The results showed no significant differences for the gallic acid equivalent for all samples obtained from the two methods at a confidence level of 97-103%, indicating that the method developed could be relied upon to analyze antioxidant activity from real samples. Finally, the paper-based antioxidant sensor is known to be stable for three days when stored in the refrigerator (2- 4 °C), stable for 2 hours at room temperature (25ºC), and makes paper sensors easy to use for end users.
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Venkatachalapathi, A., Abdul Kaffoor H, and S. Paulsamy. "In vitro antioxidant activity and polyphenol estimation of methanolic fruit extract of Carissa spinarum L." Journal of Ayurvedic and Herbal Medicine 3, no. 3 (September 30, 2017): 122–26. http://dx.doi.org/10.31254/jahm.2017.3304.
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Antioxidant property of methanolic fruit extract of the medicinal tree species, Carissa spinarum was evaluated by studying the contents of total phenolics, tannins and flavonoids, free radical scavenging activity using 1,1-diphenyl-2- picryl hydrozyl (DPPH), hydroxyl radical scavenging activity, reducing power activity, ABTS•+ assay and metal chelating activity. The results of the study revealed that both the parts studied were found to have potent antioxidant activity against DPPH, hydroxyl and ABTS•+ radicals with the IC50 value of 88.98 for methanolic fruit extract for DPPH radicals and 849.70 for hydroxyl radicals. Therefore methanolic fruit extract of C. spinarum can be considered as a new potential source of natural antioxidants for pharmaceutical industries.
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Kamath, Bindhu R., and Sabeena Kizhedath. "In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn." International Journal of Basic & Clinical Pharmacology 7, no. 5 (April 23, 2018): 849. http://dx.doi.org/10.18203/2319-2003.ijbcp20181424.
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Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.
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Swathi, Naraparaju, and Thirumurthy Durai Ananda Kumar. "Insights into Thiazolidinedione Analogues: Unveiling Antioxidant Activity through Descriptor-Based Quantitative Structure-Activity Relationship Investigations." Asian Journal of Applied Chemistry Research 15, no. 1 (January 24, 2024): 1–11. http://dx.doi.org/10.9734/ajacr/2024/v15i1277.
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Background: Diabetes mellitus, a chronic metabolic disorder is characterized by defective insulin secretion (β-cell dysfunction), insulin action (insulin resistance) and reduced bio-antioxidant potential. Antioxidants play key role in diabetes by reducing the oxidative stress and alleviating diabetic complications. Thiazolidinediones (TZDs) attenuate insulin resistance and produce antioxidant effect. Study Design: In continuation of our goal to develop thiazolidine-2,4-dione analogues that can address both oxidative stress and Type 2 diabetes, the in vitro antioxidant potential of a few synthesized thiazolidinediones (1-61) were evaluated for their DPPH and nitric oxide free radical scavenging assays. Descriptor-based QSAR analysis was utilized to study the structural contribution to the radical scavenging potential. Results: Among all test compounds, the DPPH radical scavenging activity of compound 12 was found to be significant (IC50 22.7±0.43 μM). The compound 11 (IC50: 13.8±0.5 μM) showed superior nitric oxide radical scavenging potential, when compared to ascorbic acid (IC50: 14.8±0.7 µM). Among various developed QSAR models, 16 and 29 models were found to be best for DPPH and nitric oxide radical scavenging activities, respectively. The R2 value 0.745 and 0.890 in the above models are indicative of good correlation between in vitro and in silico antioxidant activity. Conclusion: The QSAR studies revealed the potential contribution of the partition-coefficient, hydrogen bond acceptor and donors and molecular weight towards the antioxidant activity in both the assay models.
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Hendri Faisal and Sri Handayani. "Comparison of Antioxidant Activity of Ethanol Extract of Fruit and Okra Leaves (Abelmoschus esculentus L. Moench) with DPPH and ABTS Methods." Indonesian Journal of Pharmaceutical and Clinical Research 2, no. 2 (December 30, 2019): 6–13. http://dx.doi.org/10.32734/idjpcr.v2i2.2815.
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Excessive free radical activity in the human body can be characterized by pathological conditions such as cardiovascular disease, pre-natal complications, arthritis, cataracts, parkinsonism, alzheimer's, and aging. One way to prevent the formation of free radicals is to use nutrients that act as antioxidants. Natural antioxidants found in fruits and vegetables are effective free radicals and are considered non-toxic when compared with synthetic antioxidants, one of which is the okra plant Abelmoschus esculentus L.The purpose of this study is to determine differences in the antioxidant activity ethanol extract of fruit and okra leaves with DPPH method and ABTS method. The method used in this study is the maceration extraction method with 70% ethanol solvent to obtain fruit extracts and okra leaves. The antioxidant activity test was carried out by the DPPH (1,1-diphenyl-2-picrylhydrazi) method by measuring the absorption of a mixture of sample extracts and DPPH by UV-Visible spectrophotometry at a wavelength of 520 nm and by the ABTS method ((2,2-azinobis- (3 -Ethylbenzothiazoline-6-Sulfonic Acid) by measuring the absorption of a mixture of sample extracts and ABTS by UV-Visible spectrophotometry at a wavelength of 734 nm. measurement of antioxidant activity of okra fruit ethanol extract by ABTS method has very strong antioxidant activity (IC50 24.50 ppm)
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Aicha, Brahim Ben, Rachid Rouabhi, Salim Gasmi, Chawki Bensouici, Hichem Mohammedi, and Imad Mennai. "LC-MS Analysis and Antioxidant Activity of the Hydro-alcoholic Extract of Melissa Officinalis L. From Algeria." Chemistry Journal of Moldova 15, no. 2 (December 2020): 78–87. http://dx.doi.org/10.19261/cjm.2020.778.
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The present work focuses on evaluation of the chemical composition and antioxidant activity of the hydro-methanolic extract of Melissa officinalis from Algeria. The liquid chromatography-mass spectrometry analysis allowed the identification of six compounds: caffeic acid, caftaric acid, hydroxyjasmonic acid glucoside, caftaric acid glucoside, rosmarinic acid and sagerinic acid. The in-vitro antioxidant activity of the hydro-methanolic extract was evaluated by using four different methods including: radical scavenging assay (DPPH), scavenging activity (ABTS), cupric reducing antioxidant capacity, and ferric reducing power assay. The extract exhibited a relatively strong antioxidant activity compared to the synthetic antioxidants. The highest radical scavenging activity was registered using DPPH and ABTS methods, IC50= 20.53±2.64 μg/mL and 22.50±0.67 μg/mL, respectively. These results suggest that Melissa officinalis L. could be considered a potential source of natural antioxidants with potential interest in the agrochemical and pharmaceutical industries.