Relevant bibliographies by topics / Dopamine detection

Academic literature on the topic 'Dopamine detection'

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Published: 9 March 2023
Last updated: 10 March 2023

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Journal articles on the topic "Dopamine detection"

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Cemgil Sultan, Sinan, Esma Sezer, Yudum Tepeli, and Ulku Anik. "Centri-voltammetric dopamine detection." RSC Adv. 4, no. 59 (2014): 31489–92. http://dx.doi.org/10.1039/c4ra04887c.

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Abu-Ali, Hisham, Cansu Ozkaya, Frank Davis, Nik Walch, and Alexei Nabok. "Electrochemical Aptasensor for Detection of Dopamine." Chemosensors 8, no. 2 (April 15, 2020): 28. http://dx.doi.org/10.3390/chemosensors8020028.

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This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer labeled with an electrochemically active ferrocene tag. Aptamers immobilized on the surface of gold screen-printed gold electrodes via thiol groups can change their secondary structure by wrapping around the target molecule. As a result, the ferrocene labels move closer to the electrode surface and subsequently increase the electron transfer. The cyclic voltammograms and impedance spectra recorded on electrodes in buffer solutions containing different concentration of dopamine showed, respectively, the increase in both the anodic and cathodic currents and decrease in the double layer resistance upon increasing the concentration of dopamine from 0.1 to 10 nM L−1. The high affinity of aptamer-dopamine binding (KD ≈ 5 nM) was found by the analysis of the binding kinetics. The occurrence of aptamer-dopamine binding was directly confirmed with spectroscopic ellipsometry measurements.
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MORA-FERRER, CARLOS, and VOLKER GANGLUFF. "D2-dopamine receptor blockade impairs motion detection in goldfish." Visual Neuroscience 17, no. 2 (March 2000): 177–86. http://dx.doi.org/10.1017/s0952523800171196.

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Under photopic illumination conditions, motion detection in goldfish is dominated by the long-wavelength-sensitive cone type (L-cone), and under scotopic conditions motion it is determined by rods (Schaerer & Neumeyer, 1996). The switch from rod-dominated to cone-dominated motion detection occurs during light adaptation. It has been suggested that dopamine acts as a neuronal light-adaptative signal. It is known that dopamine affects wavelength discrimination through D1-dopamine receptors (Mora-Ferrer & Neumeyer, 1996), and the dorsal light reflex through D1- and D2-dopamine receptors (Lin & Yazulla, 1994a). The purpose of this study was to determine whether dopamine influenced movement detection by goldfish, and if so, which dopamine receptor was involved. The D2-dopamine receptor antagonist sulpiride reduced the animal's sensitivity to the moving stimulus, whereas SCH 23390, a D1-dopamine receptor antagonist, did not have any effect. The effect of sulpiride is discussed in relation to known sulpiride effects on retinal neurons and the retinal pigment epithelium.
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Byington, Keith H. "Detection of dopamine-tissue adducts." Life Sciences 63, no. 1 (May 1998): 41–44. http://dx.doi.org/10.1016/s0024-3205(98)00234-3.

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Ortega, Fidel, and Elena Domínguez. "Selective catalytic detection of dopamine." Journal of Pharmaceutical and Biomedical Analysis 14, no. 8-10 (June 1996): 1157–62. http://dx.doi.org/10.1016/s0731-7085(96)01720-7.

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Azharudeen, A. Mohamed, Arpita Roy, R. Karthiga, S. Arun Prabhu, M. G. Prakash, A. Mohamed Ismail Badhusha, Huma Ali, Khadijah Mohammedsaleh Katubi, and Md Rabiul Islam. "Ultrasensitive and Selective Electrochemical Detection of Dopamine Based on CuO/PVA Nanocomposite-Modified GC Electrode." International Journal of Photoenergy 2022 (February 22, 2022): 1–9. http://dx.doi.org/10.1155/2022/8755464.

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At present, the determination of dopamine (DA) is enormously necessary for the human body. Since then, it has played a crucial role in the brain that affects mood, sleep, memory, learning, and concentration. Dopamine insufficiency is a threat to human health. Dopamine recognition is important to avoid this problem. Copper oxide (CuO) nanoparticles are one of the potentials which can be used in the detection of dopamine level in the sample. In this work, CuO was synthesized by a simple chemical precipitation technique and modified by polyvinyl alcohol (PVA) as a capping agent. The nanomaterials manufactured are used for the detection of dopamine in 0.1 M PBS medium at room temperature. The CuO/PVA-modified electrode shows better electrocatalytic activity than CuO/GCE (glassy carbon electrode). The constructed dopamine biosensor of copper oxide-PVA nanocomposites also has extraordinary selectivity, stability, sensitivity (183.12 μA mM-1 cm-2), and a minimum level detection limit of 0.017 μM, is inexpensive, and has minimal effort and rapid detection of dopamine.
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Mazurkiewicz, Wojciech, Artur Małolepszy, and Emilia Witkowska Nery. "Simultaneous Detection of Neurotransmitters Using Carbon Nanomaterials." ECS Meeting Abstracts MA2022-01, no. 53 (July 7, 2022): 2195. http://dx.doi.org/10.1149/ma2022-01532195mtgabs.

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Glassy carbon electrodes cleaned and electrochemically pretreated in PBS were compared to electrodes modified with carbon black, graphene oxide, and carbon dots in terms of possibility of detection of neurotransmiters. We provide systematic studies regarding the detection of five neurobiologically relevant species: dopamine, serotonin, epinephrine, norepinephrine, and 3,4-dihydroxyphenylacetic acid on those four types of electrodes. We also evaluated the possibility of simultaneous detection of dopamine and serotonin in the presence of high levels of uric and ascorbic acids. Dopamine and serotonin are easily detected at carbon surfaces but unfortunately at a similar potential. Polymerization of dopamine, resulting in sensitivity loss is another problem, when measurements are carried out in cell culture medium or even in model solutions at physiological pH. Norepinephrine and epinephrine are both products of dopamine metabolism. Their structure includes an o-hydroquinone, which upon oxidation and the transfer of two electrons transforms into o-quinone. Additional chemical reactions may occur resulting in e.g. cyclization of the chain containing the epinephrine’s amino group and subsequent additional quinone-hydroquinone reactions of this product. DOPAC is the product of dopamine deamination. In neutral pH, its oxidation is partially irreversible. As in case of dopamine, side reactions can lead to film formation on the electrode surface. It is considered an interferent in determining dopamine levels. Detection in the presence of acids has proven possible on all three types of electrode modifications, although with different resolution. We evaluated sensitivity in terms of current density per active area of the sensor. In this way graphene oxide exhibited the highest sensitivity towards all tested neurotransmitters, whereas chemically functionalized carbon nanodots significantly increased oxidation peaks’ resolution. A considerable shift of signals towards more negative potentials for epinephrine, norepinephrine, and 3,4-dihydroxyphenylacetic acid was observed for all modifications as compared with glassy carbon electrodes. Analytical parameters obtained for each type of modification are resumed in Tab. 1 (dopamine), Tab. 2 (serotonin) and Tab.3 (epinephrine and norepinephrine). As shown in this and other works electrode pretreatment has a huge impact on the recorded signal. Thus it is sometimes hard to compare materials tested by different research groups. The same procedure of electrochemical pretreatment was in this work applied to coated and uncoated glassy carbon electrodes. The pretreatment allowed for partial cleaning of polymerized dopamine. Authors would like to thank The National Centre for Research and Development, Poland, for funding under grants LIDER/38/0138/L-9/17/NCBR/2018 and LIDER/33/0117/L-9/17/NCBR/2018. EWN would like to also thank the Foundation for Polish Science (FNP), from which she was supported through START programme and National Science Centre Poland for the MINIATURA grant NCN 2017/01/X/ST4/00463 Figure 1
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Sobahi, Nebras, Mohd Imran, Mohammad Ehtisham Khan, Akbar Mohammad, Md Mottahir Alam, Taeho Yoon, Ibrahim M. Mehedi, Mohammad A. Hussain, Mohammed J. Abdulaal, and Ahmad A. Jiman. "Facile Fabrication of CuO Nanoparticles Embedded in N-Doped Carbon Nanostructure for Electrochemical Sensing of Dopamine." Bioinorganic Chemistry and Applications 2022 (October 14, 2022): 1–9. http://dx.doi.org/10.1155/2022/6482133.

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In the present study, a highly selective and sensitive electrochemical sensing platform for the detection of dopamine was developed with CuO nanoparticles embedded in N-doped carbon nanostructure (CuO@NDC). The successfully fabricated nanostructures were characterized by standard instrumentation techniques. The fabricated CuO@NDC nanostructures were used for the development of dopamine electrochemical sensor. The reaction mechanism of a dopamine on the electrode surface is a three-electron three-proton process. The proposed sensor’s performance was shown to be superior to several recently reported investigations. Under optimized conditions, the linear equation for detecting dopamine by differential pulse voltammetry is Ipa (μA) = 0.07701 c (μM) − 0.1232 (R2 = 0.996), and the linear range is 5-75 μM. The limit of detection (LOD) and sensitivity were calculated as 0.868 μM and 421.1 μA/μM, respectively. The sensor has simple preparation, low cost, high sensitivity, good stability, and good reproducibility.
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Fouad, Dina. "Development of a New Immunosensor for the Detection of Dopamine." Zeitschrift für Naturforschung C 62, no. 7-8 (August 1, 2007): 613–18. http://dx.doi.org/10.1515/znc-2007-7-826.

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Graphite immunoelectrodes as immunosensors using indirect immobilization of a hapten were investigated for their applicability to detect dopamine hydrochloride at low levels. Conditions were optimized to achieve the highest sensitivity using the indirect immobilization of dopamine hydrochloride through a polymerized glutaraldehyde network on microtiter plates using ELISA technique. The conditions were later transferred to the graphite rods (ø 0.8 m × 20 mm) and a comparison between the two different sensitivities (IC50 midpoint of test) was carried out. Graphite electrodes showed higher sensitivity towards dopamine than ELISA, since they were able to detect dopamine with a midpoint of test of 0.2 mmol/l while using ELISA they were able to detect dopamine hydrochloride at 2 mmol/l
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Santonocito, Rossella, Nunzio Tuccitto, Andrea Pappalardo, and Giuseppe Trusso Sfrazzetto. "Smartphone-Based Dopamine Detection by Fluorescent Supramolecular Sensor." Molecules 27, no. 21 (November 3, 2022): 7503. http://dx.doi.org/10.3390/molecules27217503.

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Supramolecular recognition of dopamine by two quinoxaline cavitands was studied in solution by fluorescence titrations, ESI-MS and ROESY measurements. In addition, the tetraquinoxaline cavitand was dropped onto a siloxane-based polymeric solid support, obtaining a sensor able to detect dopamine in a linear range of concentrations 10 Mm–100 pM, with a detection limit of 1 pM, much lower than the normal concentration values in the common human fluids (plasma, urine and saliva), by using a simple smartphone as detector. This sensor shows also good selectivity for dopamine respect to the other common analytes contained in a saliva sample and can be reused after acid–base cycles, paving the way for the realization of real practical sensor for human dopamine detection.
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Dissertations / Theses on the topic "Dopamine detection"

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Ngomane, Nokuthula. "Gold nanoparticle–based colorimetric probes for dopamine detection." Thesis, Rhodes University, 2016. http://hdl.handle.net/10962/d1021261.

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Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
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Skaf, Tania. "Development of electrochemical (Bio)sensors for the detection of dopamine." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=99540.

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The development of dopamine (DA) electrochemical sensors and biosensors is described in this thesis. The aim was to develop (bio)sensors that would continuously and specifically detect DA over a wide concentration range, with high sensitivity and low detection limits. The electrode surfaces used were platinum (Pt), boron-doped-diamond (BDD), and carbon-paste (CP). The first two were used to construct DA sensors, while Pt was shown to be superior for the construction of biosensors. The DA biosensor configurations were based on the use of a Pt electrode as a transducer surface, polyphenol oxidase (PPO) as an enzyme, ferrocene (Fc) as a mediator, and polypyrrole (PPY) and Nafion as membranes. A range of electrochemical techniques were employed in this research.
It was determined that the oxidation of DA on bare Pt is a surface-controlled reaction, occurring at low overpotentials. The reaction is electrochemically reversible, involving the spontaneous adsorption of DA on the electrode surface. The Pt and BDD sensors were efficiently used to determine DA in aqueous solutions. In order to increase their resistance to the ascorbic acid (AA) interference, the sensor surfaces were modified by a thin Nafion film. This configuration was shown to selectively detect DA even when AA was present with DA at a 1000-time larger concentration. The lowest DA detection limit was achieved using the unmodified BDD sensor, 50 nM. Nevertheless, both the unmodified and Nafion-modified Pt and BDD sensors were suitable for monitoring of DA at concentration levels typical for urine samples.
It was shown that the sensitivity and detection limit of the developed Pt-based biosensors depend on the amount of PPO and Fc incorporated into the PPY membrane, and also on their ratio. The modification of the biosensor by a Nafion membrane offered three benefits: an increase in sensitivity, an improvement in detection limit, and a significant minimization of the AA interference. An optimum biosensor architecture was made by polymerizing PPY for 40 minutes from a pyrrole solution containing 2,400 U mL-1 of PPO and 10 mM of Fc, on top of which a thin Nafion film was formed. Using chronoamperometry as a detection technique, this biosensor yielded a DA detection limit of 20 nM, which makes it suitable for monitoring DA levels in brain. Even a lower detection limit, 10 nM, and higher sensitivity were achieved by using electrochemical impedance spectroscopy (EIS) as a detection technique. Unfortunately, the developed biosensor lacked operational stability, predominately due to the leakage of PPO and Fc into the storage solution.
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Wen, Dan, Wei Liu, Anne-Kristin Herrmann, Danny Haubold, Matthias Holzschuh, Frank Simon, and Alexander Eychmüller. "Simple and Sensitive Colorimetric Detection of Dopamine Based on Assembly of Cyclodextrin-Modified Au Nanoparticles." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-210959.

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A controlled assembly of natural beta-cyclodextrin modified Au NPs mediated by dopamine is demonstrated. Furthermore, a simple and sensitive colorimetric detection for dopamine is established by the concentration-dependent assembly.
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Yeary, Amber J. "Cetyltrimethylammonium Halide-Coated Electrodes for the Detection of Dopamine in the Presence of Interferents." Wright State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=wright1323471405.

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EMVALOMENOS, Gaelle. "Quantitative Methods For Detection of Transient Changes in Endogenous Dopamine For Preclinical PET Studies." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/25710.

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This thesis investigates quantitative methods for the characterisation of dopamine (DA) release in preclinical PET. The goal was to develop methodologies that improve quantification of the PET signal and test to what extent these techniques can reliably detect subtle DA fluctuations. Ultimately, reliable quantification could help to better understand the role of DA in human brain conditions. The focus of this work is kinetic modelling, whose accuracy depends on different factors, from the quality of the measured imaging data to the interpretation of parameter estimates. An investigation of the model, linear parametric neurotransmitter PET (lp-ntPET), that enables the description of time-varying DA changes, was performed in 3 contexts. First, its performance was tested in a Bayesian framework, the PET-ABC method. Second, it was evaluated in 1D-simulations by varying the activations and the priors, to study their effects on uncertainty in the estimates. Third, a [11C]raclopride study was performed in monkeys using the high sensitivity MiniExplorer, where different amphetamine doses were injected to induce variable DA releases. Finally, towards exploiting the synergies of PET/fMRI data in the future, a preclinical PET insert was evaluated in 3 configurations: as a standalone unit, in the MR bore and with the MR pulsing. In conclusion, the results of the studies showed that the lp-ntPET model used in conjunction with PET-ABC is a robust quantitative method that not only provides parameter estimates and their uncertainties but also statistically sound inferences about model preference. Further, PET-ABC is able to reliably estimate parameters describing transient DA release in preclinical studies. The PET insert presented good performance in all configurations, enabling future investigation of parallel analysis of fMRI and PET data for improved parameter estimation.
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Rashid, Mamun-Ur. "Development of miniaturized electro-analytical approach for dopamine and catechol determination in the presence of ascorbic acid." Thesis, Teesside University, 2013. http://hdl.handle.net/10149/312859.

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We have investigated electropolymerisation for fabrication of a chemically modified working electrode for the determination of dopamine and catechol neurotransmitters in the presence of ascorbic acid. A variety of film compositions were investigated that would allow discrimination of the neurotransmitters through a combination of electrostatic barrier and the film porosity. The films investigated were based on different compositions of () poly-o-toluidine-co-aniline (POT-co-PA), () poly-o-toluidine-co-o-anisidine (POT-co-POA) and () polyacriflavine (PAF). The POT-co-PA and POT-co-POA gave the most promising result although the POT-co-PA was preferred because of higher current enhancement and better separation of dopamine and catechol neurotransmitters in the presence of ascorbic acid. The uses of electropolymerisation make the investigated films attractive candidates for the fabrication of a chemically modified microelectrode with application in capillary electrophoresis separation with electrochemical detection. The active area of nano particle (Au, Pt and Ag) screen printed electrodes was determined using cyclic voltammogram with ferro/ferricyanide couple. The active surface of the nano particle coated electrode was found surprisingly to be 5% - 65% lower than that geometrically calculated surface area for the electrode. This is ascribed to the limitation of the screen printing approach that was used. A low cost high replication approach that would allow development of a capillary electrophoresis microfluidic chip with electrochemical detection (CE-ECD) on a polymer substrate was investigated. A fluidic top layer was fabricated using hot embossing and an electrode bottom layer by metal patterning on a polymer substrate using metallisation and photolithography.
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Guntupalli, Bhargav. "Nanomaterial-Based Electrochemical and Colorimetric Sensors for On-Site Detection of Small-Molecule Targets." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3488.

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An ideal biosensor is a compact and in-expensive device that is able to readily and rapidly detects different types of analytes with high sensitivity and specificity. The affectability of a biosensing methodology is subject to the limit of nanomaterials to transduce the target binding process to an improved perceptible signal, while the selectivity is accomplished by considering the binding and specificity of certain moieties to their targets. Keeping these requirements in mind we have chosen nanomaterials such as carbon nanotubes (CNTs) and gold nanoparticles (AuNPs) that has catalytic properties combined with their size, shape and configuration dependent chemical and physical properties as essential precursors and signaling components for creation of biosensors with tremendous sensitivity. The primary goal of the research work described in this dissertation is to develop and evaluate novel methods to detect various analytes using nanomaterials, at the same time making an affordable architecture for point-of-care (POC) applications. We report here in chapter 3 a simple and new strategy for preparing disposable, paper-based, porous AuNP/M-SWCNT hybrid thin gold films with high conductivity, rapid electron transfer rates, and excellent electrocatalytic properties to achieve multiple analyte electrochemical detection with a resolution that greatly exceeds that of purchased flat gold slides. We further explored the use of nanomaterial-based paper films in more complex matrices to detect analytes such as NADH, which can act as a biomarker for certain cellular redox imbalances and disease conditions. Carbon nanotubes with their large activated surfaces and edge-plane sites (defects) that are ideal for performing NADH oxidation at low potentials without any help of redox mediators minimizing surface fouling in complex matrices is described in chapter 4. With an instrument-free approach in mind we further focused on a colorimetric platform using split cocaine aptamers and gold nanoparticles (AuNPs) to detect cocaine for on-site applications as described in chapter 5. In chapter 5, the split aptamer sequences were evaluated mainly on three basic criteria, the hybridization efficiency, specificity towards the analyte (cocaine), and the reaction time to observe a distinguishable color change from red to blue. The assay is an enzyme-assisted target recycling (EATR) strategy following the principle that nuclease enzyme recognizes probe–target complexes, cleaving only the probe strand releasing the target for recycling. We have also studied the effect of the number of binding domains with variable chain lengths on either side of the apurinic (AP) site. On the basis of our results, we finally shortlisted the sequence combination with maximum signal enhancement fold which is instrumental in development of colorimetric platform with faster, and specific reaction to observe a distinctive color change in the presence of cocaine.
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Yapo, Cédric. "Adaptations de la cascade de signalisation AMPc/PKA dans le striatum au cours de la maladie de Parkinson et de son traitement par la L-DOPA : étude par imagerie de biosenseurs sur un modèle animal Detection of phasis dopamine by D1 and D2 striatal medium spiny neurons Switch-like PKA responses in the nucleus of striatal neuron." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS603.

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Les signaux neuromodulateurs induisent une adaptation des fonctions neuronales par le biais de mécanismes d’intégration dynamiques complexes. Parmi les voies de signalisation intracellulaires, celle de l’AMPc/PKA joue un rôle essentiel dans la réponse cellulaire à la dopamine. Pour analyser ces processus d’intégration, nous combinons l’imagerie de biosenseurs dans des préparations ​ex vivo de tranches de cerveau de souris avec de la modélisation de la signalisation intracellulaire dans les neurones D1 et D2 striataux. Dans une première partie de mon travail de thèse, nous analysons la dynamique de la signalisation striatale en réponse à des stimulations dopaminergiques transitoires telles celles associées aux récompenses. Nous montrons par imagerie que, contrairement à ce qui est communément admis, les récepteurs D​2 à la dopamine permettent la détection de dopamine phasique au niveau de l’AMPc. De plus, les simulations suggèrent que les neurones D2 pourraient détecter une diminution du niveau de dopamine tonique, indicateur d’une situation aversive chez l’animal. Ce travail a fait l’objet d’une publication (​Yapo et al., ​J. Physiol 2017​). Dans une deuxième partie, nous avons analysé l’effet dans le noyau de ces stimulations dopaminergiques rapides. En comparaison avec les neurones du cortex, nous montrons que les neurones du striatum disposent d’un mécanisme de contrôle en-avant (“​feed forward​”) qui renforce les réponses PKA nucléaires. Cette situation originale, à l’opposé des rétrocontrôles homéostatiques habituels en biologie, amène à une réponse du noyau tout ou rien, extrêmement sensible. Nous pensons que ce mécanisme est impliqué dans la détection des signaux dopaminergiques transitoires. Ce travail a été publié dans un article (​Yapo et al., ​J Cell Science​ 2018​). Enfin une troisième partie, sous forme de résultats préliminaires, consistait à analyser l’adaptation des neurones du striatum à la perte des afférences dopaminergiques, caractéristique de la maladie de Parkinson. Nous avons observé l’hypersensibilité à la dopamine affectant les neurones D1, largement décrite dans la littérature. De plus, nous montrons que les neurones du striatum présentent une activité phosphodiestérase accrue. Une meilleure compréhension de ces adaptations pathologiques pourrait mener à de nouvelles stratégies thérapeutiques
Neuromodulatory signals trigger adaptations in neuronal functions via complex integrative properties. Among the various existing intracellular signaling pathways, the cAMP/PKA cascade plays a critical role in the cellular response to dopamine. To analyze these integrative processes, we combine biosensor imaging in mouse brain slices with in silico modelisation of the intracellular signaling in D1 and D2 medium-sized spiny neurons. In a first part of my thesis work, we analyze the dynamics of cAMP/PKA signaling in striatal neurons stimulated by transient dopaminergic signals, such as those associated with reward. With imaging we show that the dopamine D​2 receptors can sense phasic dopamine signals at the level of cAMP, a thought that has been argued for long. Moreover ​in silico simulations suggest that D2 spiny neurons could sense the interruptions in tonic dopamine levels associated with aversion in the animal. This work was published in (​Yapo et al., ​J Physiol 2017​). In a second part, we analyzed the effect of such brief dopaminergic signals on the nuclear PKA-dependent signaling. In comparison to cortical neurons, we show that the striatal neurons display a positive feedforward mechanism which strengthens the nuclear responses. This peculiar situation, which contrasts with the usual homeostatic feedback mechanisms found in biology, leads to all-or-nothing and extremely sensitive responses. We believe that this mechanism allows for the detection of transient dopaminergic signals. This work was published in (​Yapo et al., ​J Cell Science​ 2018​). Lastly a third part, that will be introduced as preliminary data, consisted in analyzing the adaptations of the striatal neurons following a dopamine depletion, such as the one found in Parkinson’s disease. We observed in our mouse model an hypersensitivity of the D1 spiny neurons to dopamine, already described by other groups. Additionally we show that striatal neurons display an increased phosphodiesterase activity. A better understanding of these pathological adaptations could lead to the emergence of new therapeutic strategies
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Patel, Mohit Pratish. "OPTIMIZATION AND APPLICATION OF PHOTOLUMINESCENCE- FOLLOWING ELECTRON-TRANSFER WITH TRIS(TETRAMETHYL- 1,10-PHENANTHROLINE) Os/Ru(III) COMPLEXES AND FENTON BASED CHEMILUMINESCENCE DETECTION OF NSAIDS AND DOPAMINE IN BIOLOGICAL SAMPLES." Diss., Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/385393.

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Chemistry
Ph.D.
Biogenic monoamines such as dopamine play an important role as major neurotransmitters. Simultaneous determination of the concentration changes is thus crucial to understand brain function. Additionally, quantification of pharmaceutically active compounds (PhACs) and their metabolites in biological fluids is an important issue for forensic tests, clinical toxicology and pharmaceutical analysis. We have developed two postcolumn luminescence detection methods coupled to a 2-dimensional-solid phase extraction (2D-SPE) system. The postcolumn reaction methods used in this study are the redox-dependent photoluminescence-following electron-transfer (PFET) and Fenton-based chemiluminescence techniques, for the determination of certain neurotransmitter and nonsteroidal anti-inflammatory drugs (NSAIDs). A stable [Os(tmphen)3]3+ (tmphen = 3,4,7,8-tetramethyl-1,10-phenanthroline) reagent was prepared in neutral aqueous solution by oxidation of [Os(tmphen)3]2+ with lead(IV) oxide. [Os(tmphen)3]2+ and [Os(tmphen)3]3+ are characterized by absorption spectroscopy. [Os(tmphen)3]3+ stability is compared with [Ru(tmphen)3]3+ in the same pH 7 environment. The properties of Os(III) and Ru(III) complexes were investigated for use as the oxidant in a PFET system. Studies of photophysical and electrochemical properties, the stability of the Os(III) and Ru(III) complexes, and analytical application in PFET detection of oxidizable analytes are presented. The spectroscopic properties of the complexes were not very advantageous, but careful control of the detection system and reaction conditions enabled sensitive detection of the analytes. The method was fully validated and the optimized system was capable of detecting dopamine and acetaminophen at about 30.2 µg L-1 and 33.5 µg L-1, respectively. The limit of detection (LOD) was 1.5 µg L-1 for acetaminophen and 4.3 µg L-1 for dopamine. The accuracy and precision were within bioanalytical method validation limits (90.9 to 101.5 % and RSD < 12.0 %, respectively). Typical analysis time was less than 15 minutes. Two Fenton-based flow-injection chemiluminescence (CL) methods were developed and validated for the determination of naproxen. Under the optimal experimental conditions the proposed methods exhibited advantages in a larger linear range from 2,760 ng mL-1 to 207,000 ng mL-1 for the first CL method and 41.4 ng mL-1 to 700.0 ng mL-1 for the second CL method. The LOD was 13.8 ng mL-1 for naproxen. The CL mechanisms for the system, H2O2-FeIIEDTA-naproxen was further studied by batch experiments, chemiluminescence spectroscopy, fluorometry, high pressure liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). The effects of various interferences commonly found in biological and wastewater systems on the chemiluminescence intensity were also investigated. We used these methods to determine NSAIDs in commercial pharmaceutical formulations. Another application of these method was for detecting NSAIDs in biological samples. A 2x-1-Dimensional Solid Phase Extraction (2x-1D SPE) method was developed for determination of acetaminophen and naproxen in urine. This method uses both the methanol concentration and the pH advantageously to preferentially isolate analytes of interest from complex sample matrix. These methods were fully validated and had sufficient sensitivity (limit of quantification: acetaminophen; 40.41 mg L-1 - 360.0 mg L-1 and naproxen; 23.03 mg L-1 - 214.8 mg L-1) for biological matrices and applications.
Temple University--Theses
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Kollipara, Suresh Babu. "Organic Electrochemical Transistors for Fast Scan Cyclic Voltammetry." Thesis, Linköpings universitet, Fysik och elektroteknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-98676.

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The work presented in the thesis is about the evaluation of Organic Electrochemical Transistors (OECTs) for fast scan cyclic voltammetry (FSCV). FSCV is a method which has been used for real time dopamine sensing both in vivo and in vitro. The method is sensitive to noise and could therefore benefit from signal preamplification at the point of sensing, which could be achieved by incorporation of OECTs. In this study the OECTs are based on the conductive polymer poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS). The gate consists of gold microelectrodes of different sizes to be used one at a time. When dopamine is reacted at the gate electrode, the redox state of the PEDOT:PSS OECT channel is modulated and the resulting change in drain current can be measured. The gate current, which contains the sensing information, is after filtering obtained by differentiating the channel potential with respect to time. The derived gate current is plotted in cyclic voltammogram for different dopamine concentrations and the amplitude of the oxidation/reduction peaks can be used to determine the dopamine concentration. In this thesis for the first time it is demonstrated that OECTs can be used for FSCV detection of dopamine. The results are discussed and an outlook on future work is given.
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Books on the topic "Dopamine detection"

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Beninger, Richard J. Neuroanatomy and dopamine systems. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198824091.003.0011.

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Neuroanatomy and dopamine systems explains how sensory signals ascend the central nervous system via a series of nuclei; axons detecting specific elements converge onto higher-order neurons that respond to particular stimulus features. Assemblies of feature-detection cells in the cerebral cortex detect complex stimuli such as faces. These cell assemblies project to motor nuclei of the dorsal and ventral striatum where they terminate on dendritic spines of efferent medium spiny neurons. Dopaminergic projections from ventral mesencephalic nuclei terminate on the same spines. Individual corticostriatal afferents contact relatively few medium spiny neurons and individual dopaminergic neurons contact a far larger number. Stimuli activate specific subsets of corticostriatal synapses. Synaptic activity that is closely followed by a rewarding stimulus, that produces a burst of action potentials in dopaminergic neurons, is modified so that those specific corticostriatal synapses acquire an increased ability to elicit approach and other responses in the future, i.e., incentive learning.
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Roze, Emmanuel, and Nenad Blau. Biogenic Monoamine Disorders. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199972135.003.0031.

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Biogenic monoamine disorders are a group of inherited diseases characterized by a defect in the synthesis, transport, or degradation of catecholamines and serotonin. The phenotype mostly reflects the pattern and severity of the monoamine deficiency. Movement disorders due to cerebral dopamine deficiency are almost always prominent, mostly in the form of dystonia and/or parkinsonism. These disorders are potentially devastating yet treatable. Early diagnosis and treatment are crucial to prevent ongoing brain dysfunction. Detection of hyperphenylalaninemia in a neonate could be a good clue to the diagnosis. Final diagnosis is often based on a detailed biochemical investigation of the cerebrospinal fluid and can be confirmed by molecular analysis. Treatment is aimed at restoring neurotransmitter homeostasis using monoamine precursors, monoamine agonists, and inhibitors of monoamine degradation. It also comprises the control of hyperphenylalaninemia and the prevention of cerebral folate deficiency, when applicable.
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Arnold, Monica M., Lauren M. Burgeno, and Paul E. M. Phillips. Fast-Scan Cyclic Voltammetry in Behaving Animals. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0005.

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Gaining insight into the mechanisms by which neural transmission governs behavior remains a central goal of behavioral neuroscience. Multiple applications exist for monitoring neurotransmission during behavior, including fast-scan cyclic voltammetry (FSCV). This technique is an electrochemical detection method that can be used to monitor subsecond changes in concentrations of electroactive molecules such as neurotransmitters. In this technique, a triangular waveform voltage is applied to a carbon fiber electrode implanted into a selected brain region. During each waveform application, specific molecules in the vicinity of the electrode will undergo electrolysis and produce a current, which can be detected by the electrode. In order to monitor subsecond changes in neurotransmitter release, waveform application is repeated every 100 ms, yielding a 10 Hz sampling rate. This chapter describes the fundamental principles behind FSCV and the basic instrumentation required, using as an example system the detection of in vivo phasic dopamine changes in freely-moving animals over the course of long-term experiments. We explain step-by-step, how to construct and surgically implant a carbon fiber electrode that can readily detect phasic neurotransmitter fluctuations and that remains sensitive over multiple recordings across months. Also included are the basic steps for recording FSCV during behavioral experiments and how to process voltammetric data in which signaling is time-locked to behavioral events of interest. Together, information in this chapter provides a foundation of FSCV theory and practice that can be applied to the assembly of an FSCV system and execution of in vivo experiments.
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Book chapters on the topic "Dopamine detection"

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Xiao, Jiping, and Clare Bergson. "Detection of Cell Surface Dopamine Receptors." In Methods in Molecular Biology, 3–13. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-251-3_1.

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Tertiș, M., A. Florea, A. Adumitrachioaie, D. Bogdan, C. Cristea, and R. Săndulescu. "New Approach for the Electrochemical Detection of Dopamine." In International Conference on Advancements of Medicine and Health Care through Technology; 12th - 15th October 2016, Cluj-Napoca, Romania, 103–6. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-52875-5_23.

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Hu, Yuwei, Fenghua Li, Dongxue Han, and Li Niu. "Graphene for Glucose, Dopamine, Ascorbic Acid, and Uric Acid Detection." In SpringerBriefs in Molecular Science, 57–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-45695-8_4.

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Spadaro, S., Enza Fazio, Martina Bonsignore, N. Lavanya, C. Sekar, S. G. Leonardi, F. Neri, and G. Neri. "Electrochemical Sensor Based on Molybdenum Oxide Nanoparticles for Detection of Dopamine." In Lecture Notes in Electrical Engineering, 31–38. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-04324-7_5.

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Navarro, Gemma, Peter J. McCormick, Josefa Mallol, Carme Lluís, Rafael Franco, Antoni Cortés, Vicent Casadó, Enric I. Canela, and Sergi Ferré. "Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET Technology." In Methods in Molecular Biology, 95–105. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-251-3_7.

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Rashid, M., V. Auger, and Z. Ali. "Simultaneous Electrochemical Detection of Dopamine, Catechol and Ascorbic Acid at a Poly(acriflavine) Modified Electrode." In IFMBE Proceedings, 892–95. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-00846-2_221.

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Demirkan, Buse, Hasan Ay, Sümeyye Karakuş, Gülseren Uzun, Anish Khan, and Fatih Şen. "Electrochemical Detection of Dopamine in the Presence of Uric Acid Using Graphene Oxide Modified Electrode as Highly Sensitive and Selective Sensors." In Carbon Nanostructures, 179–92. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-32-9057-0_7.

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Bert, Lionel, Valérie Martin, Laura Lambas-Señas, Marie-Françoise Suaud-Chagny, and Bernard Renaud. "Determination of Subnanomolar Concentrations of Dopamine and Norepinephrine in Nanovolume Samples Using an Automated Capillary Zone Electrophoresis with Laser Induced Fluorescence Detection." In Catecholamine Research, 309–12. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4757-3538-3_73.

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Chen, G. C., H. Z. Han, T. C. Tsai, C. C. Cheng, and J. J. Jason Chen. "Voltammetric Approach for In-vivo Detecting Dopamine Level of Rat’s Brain." In IFMBE Proceedings, 367–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-21729-6_95.

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Roogi, Jyoti M., and Dr Manju Devi. "Machine Learning based CMOS Readout Circuit for Advance Detection of Parkinson’s Disease." In Applications of Artificial Intelligence and Machine Learning in Healthcare. Technoarete Publishing, 2022. http://dx.doi.org/10.36647/aaimlh/2022.01.b1.ch004.

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An Organic chemical called Dopamine belongs to catecholamine and phenethylamine chemical families. A neurotransmitter chemical released by neurons and is one of prime function of Dopamine in the brain. They are essential in communicating messages for all parts of the brain and between the brain and body organs. Body movement is controlled by dopamine. A lack of or an insufficient dopamine generation in part of the brain can lead to Parkinson ’s Disease (PD). It is a one of neurological disorder that affects body movement. It may cause stiffness, trembling in body parts. Detection of low level dopamine is challenging and complex as the low level of dopamine is related to Parkinson’s disease. In this chapter we present an approach towards early detection of this neurological disorder PD by employing CMOS readout circuit which measures and amplifies low level dopamine in the form of electrical signal from brain with help of electrodes.ADC is used to convert amplified analog signal to digital information. Machine learning algorithms are used to predict the disease based on the data received from the readout circuit. Dopamine level is measured in current which ranges from pA to nA. CMOS Amplifiers are used to strengthen the acquired signal in the range of millivolts (mV) with the help of bio amplifiers. For conversion of acquired current in the range of pA to voltage with amplitude of micro voltage (µV) CMOS front called Transimpedance amplifier (TIA) is employed. This chapter provides complete design and analysis of low noise, low power CMOS machine learning based readout circuit for detection and prediction of Parkinson’s disease.
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Conference papers on the topic "Dopamine detection"

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Ghosh, Dipannita, Md Ashiqur Rahman, Ali Ashraf, and Nazmul Islam. "Graphene-Conductive Polymer-Based Electrochemical Sensor for Dopamine Detection." In ASME 2022 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/imece2022-96193.

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Abstract The central nervous system’s (CNS) dopaminergic system dysfunction has been linked to neurological illnesses like schizophrenia and Parkinson’s disease. As a result, sensitive and selective detection of dopamine is critical for the early diagnosis of illnesses associated with aberrant dopamine levels. In this research, we have investigated the performance of electrochemical screen-printed sensors for different concentrations of dopamine detection using graphene-based conductive PEDOT: PSS(G-PEDOT: PSS) and Polyaniline(G-PANI) inks on the working electrode and compared the sensitivity. SEM characterization technique has been performed to visualize the microstructures of the proposed inks. We have investigated cyclic voltammetry (CV) electrochemical techniques with ferri/ferrocyanide redox couple to assess the efficiency of the designed electrodes in detecting dopamine. G-PANI ink has shown to have better LOD and stability to detect dopamine with screen-printed electrodes. Further, we have also studied electrochemical analysis for the selective detection of dopamine without the interference of Ascorbic Acid (AA).
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Giordani, Martina, Michele Di Lauro, Marcello Berto, Carlo A. Bortolotti, Dominique Vuillaume, Henrique L. Gomes, Michele Zoli, and Fabio Biscarini. "Whole organic electronic synapses for dopamine detection." In SPIE Organic Photonics + Electronics, edited by Ioannis Kymissis, Ruth Shinar, and Luisa Torsi. SPIE, 2016. http://dx.doi.org/10.1117/12.2239532.

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Singh, Anshul, K. Kulathuraan, K. Pakiyaraj, Vasu Gajendiran, Devesh Pratap Singh, and Kalpana Sengar. "Green Synthesized Carbon Quantum Dots from Curcuma Longa for Ascorbic Acid Detection." In International Conference on Recent Advancements in Biomedical Engineering. Switzerland: Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-7t5606.

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In this study, we developed a carbon-dot-based sensor, which is particularly sensitive to ascorbic acid. It was possible to generate carbon dots (CDs) by utilising a renewable resource: Curcuma longa, which is abundantly available. The carbon dots produced from curcuma longa have particle diameters of 0.6 nm and are extremely brilliant in appearance. It has been proven that the fluorescence of carbon dots is inhibited in the presence of dopamine and ascorbic acid, with dopamine being more sensitive to the fluorescence than ascorbic acid. There were no significant differences between the minimal detection limits for dopamine and ascorbic acid, which were 33 μM, respectively. The Stern-Volmer plot was used to establish the quenching of ascorbic acid. It is one of the potential technique for sensing ascorbic acid.
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Zhan, Feng-Lin, Li-Min Kuo, Shi-Wei Wang, Michael S. C. Lu, Wen-Ying Chang, Chih-Heng Lin, and Yuh-Shyong Yang. "An Electrochemical Dopamine Sensor with CMOS Detection Circuit." In 2007 IEEE Sensors. IEEE, 2007. http://dx.doi.org/10.1109/icsens.2007.4388686.

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Chen, Lei-Guang, and Michael S. C. Lu. "Class-based CMOS capacitive sensors for dopamine detection." In 2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2011. http://dx.doi.org/10.1109/nems.2011.6017480.

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Lee, Ho Kyung, and Sang Joon Park. "Preparation of Cu2O@apoferritin for detection of dopamine." In 2017 22nd Microoptics Conference (MOC). IEEE, 2017. http://dx.doi.org/10.23919/moc.2017.8244580.

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Chang-hwan Seo and Jong-sung Kim. "Detection of dopamine via FRET between Alexa Fluors." In 2010 IEEE 3rd International Nanoelectronics Conference (INEC). IEEE, 2010. http://dx.doi.org/10.1109/inec.2010.5425172.

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Park, Sei Jin, Anna Ivanovskaya, and Allison Yorita. "Synthesis and Fabrication of Single Walled Carbon Nanotube Microelectrode Arrays on Flexible Probes for Neurotransmitter Detection." In ASME 2022 17th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/msec2022-85273.

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Abstract Implantable microelectrode arrays are an effective method for understanding neurotransmitter dynamics with high spatial resolution. In particular, carbon-based electrodes are efficient for electrochemical detection of dopamine, a neurotransmitter studied for its role in motor movement and reward-seeking behavior. However, very few options exist for arrayed carbon microelectrodes, specifically on flexible polymeric probes. We demonstrate fabrication of polyimide probes featuring single walled carbon nanotube (SWCNT) microelectrode arrays and characterize their dopamine detection performance. First, SWCNT synthesis parameters were optimized to grow high density SWCNT “forests” that have uniform height with electrode diameters ranging from 15 μm to 100 μm, as these dimensions are spatially relevant to chemical sensing in an animal model. These SWCNT microelectrodes were then incorporated into a microfabrication process involving deposition and patterning of polyimide substrate and metal traces. The process flow was designed such that the polyimide was not exposed to the high temperatures required to grow SWCNTs. Instead, a bottom-up approach was utilized, in which the SWCNT catalyst was first patterned, the SWCNTs were synthesized on a silicon substrate, then polyimide and trace metal layers were deposited and patterned. Prototype probes were fabricated containing the same range of electrode diameters as those used for SWCNT synthesis development to determine the effect of electrode diameter on ease of microfabrication. Microelectrodes ranging from 15 μm to 50 μm in diameter were found to release from the carrier wafer more easily, while larger electrodes demonstrated poor release. These probes demonstrate a concentration-dependent response to dopamine, with high sensitivity compared to microelectrode arrays consisting of bare metal. Further development of this electrode material will enable neuroscientists to study dopamine at higher spatial resolution, with the benefit of utilizing flexible probes.
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Wang, Shi-Wei, Chih-Heng Lin, Yuh-Shyong Yang, and Michael S. C. Lu. "A CMOS capacitive dopamine sensor with Sub-nM detection resolution." In 2009 IEEE Sensors. IEEE, 2009. http://dx.doi.org/10.1109/icsens.2009.5398250.

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Yang, Po-Hung, and Michael S. C. Lu. "An 8×8 CMOS microelectrode array for electrochemical dopamine detection." In 2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2011. http://dx.doi.org/10.1109/nems.2011.6017396.

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