Academic literature on the topic 'DNA hybrides'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'DNA hybrides.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "DNA hybrides"
1
Levy, A. V., and A. S. Ageeva. "Production of male fertile interspecific hybrides between cultivated potatoes and valuable for breeding allotetraploid species Solanum stoloniferum." Proceedings of the National Academy of Sciences of Belarus, Biological Series 64, no. 2 (May 18, 2019): 202–9. http://dx.doi.org/10.29235/1029-8940-2019-64-2-202-209.
Full textAbstract:
The germplasm of valuable for breeding wild allotetraploid potato species Solanum stoloniferumis rarely used because of pre- and postzygotic reproductive barriers with cultivated potatoes. One of the factors that complicate crosses between S. stoloniferum and S. tuberosumis unilateral incompatibility (UI).Here, we present the results of application of original SvSv-lines for overcoming UI in crosses with S. stoloniferum and of generating male fertile hybrids derived from this species. SvSv-lines are F2 S. tuberosum dihaploid× S. verrucosum that are male fertile and have D/γ-type cytoplasm. Since they are hybrids on homozygous for Svgene from S. verrucosum, they do not form SvSv-lines and have the same ability for elimination of prezygotic incompatibility as this species.As a result of pollination seven SvSv-lines were pollinated by 26 accessions of S. stoloniferum and a lot of hybrid seeds have been produced.In spite of low percentage of germination (1.9 %), formed 40 seedlings of interspecific hybrids. The experiment on hybridization between SvSv-lines and S. stoloniferum has been reproduced with the accession PI205522 of the wild species, which had DNA markers of PVY and LB resistance genes and “sterile” type cytoplasm W/γ: 950 hybrid seeds and 12 viable seedlings were produced. The genome of the seedlings was doubled by colchicine treatment, which generated hexaploids (F1) that formed highly fertile pollen and set seeds from self-pollination. We were able to cross them as females with the variety Katahdin. Produced pentaploid hybrids (BC1) were readily backcrossed by potato variety Quarta. Seedlings of BC2 were then backcrossed by potato varieties as female and, some of them, as male parents. The substantial part of F1, BC1 and BC2 plants of interspecific hybrids were male fertile (produced a lot functionally fertile pollen).
2
Stack, Colin, Subbana Easwaramoorthy, Usha Metha, Martin Downes, Christine Griffin, and Ann Burnell. "Molecular characterisation of Heterorhabditis indica isolates from India, Kenya, Indonesia and Cuba." Nematology 2, no. 5 (2000): 477–87. http://dx.doi.org/10.1163/156854100509321.
Full textAbstract:
Abstract Isolates of Heterorhabditis were identified as H. indica using the following molecular diagnostic features: hybridisation to a H. indica specific satellite DNA probe; AluI and MboI restriction profiles of the rDNA ITS PCR product and the AluI profile of the rDNA IGS PCR product. The Kenyan isolates represent a distinct subgroup of H. indica. These isolates lacked one of the two HinfI restriction sites which are present in the rDNA ITS product of all the other isolates tested and they also differed from other H. indica isolates in their rDNA IGS HaeIII restriction profile. The Indian isolates are interfertile. The Kenyan isolates are interfertile but only one Kenyan isolate, Ki3, produced viable progeny when crossed with H. indica LN2. The four Indonesian isolates are interfertile, but only one Indonesian isolate (INA H1) produced viable hybrids when crossed with H. indica LN2. INA H1 was also interfertile with the Kenyan isolate Ki3. Caractérisation moléculaire d'isolats d'Heterorhabditis indica provenant d'Inde, du Kenya, d'Indonésie et de Cuba - Des isolats d'Heterorhabditis ont été identifiés comme H. indica par l'utilisation des techniques de caractérisation moléculaire suivantes: hybridation avec une sonde spécifique du DNA satellite de H. indica, produits des profils de restriction par PCR de l'ITS du rDNA par AluI et MboI et produit de PCR de l'IGS du rDNA par AluI. Les isolats keniyans constituent un sous-groupe distinct d'H. indica. Un des deux sites de restriction de HinfI, présent dans les produits de l'ITS du rDNA de tous les autres isolats étudiés, est absent dans ces isolats qui différaient également dans leurs profils de restriction de l'IGS du rDNA par HaeIII. Les isolats d'Inde sont interfertiles. Les isolats kenyans sont inter-fertiles mais un seul de ces isolats, Ki3, a produit une descendance viable après croisement avec H. indica LN2. Les quatre isolats indonésiens sont interfertiles, mais un seul d'entre eux (INA H1) a produit des hybrides viables après croisement avec H. indica LN2. INA H1 a été également interfertile avec l'isolat kenyan Ki3.
3
Kondratskaya, I. P., A. N. Yukhimuk, V. A. Stolepchenko, O. V. Chizhik, Z. G. Kozlovskaya, P. P. Vasko, and V. N. Reshetnikov. "The creating of intergenetic hybrids of festulolium of Festuca arundinacea morphotipe with the use of post-genomic technologies and DNA-marking." Faktori eksperimental'noi evolucii organizmiv 25 (August 30, 2019): 253–59. http://dx.doi.org/10.7124/feeo.v25.1172.
Full textAbstract:
Aim. To form the varietal population of festulolium intergeneric hybrids of Festuca arundinacea morphotype. To carry out DNA-labeling of created festulolium hybrid plants and parental forms. Methods. The festulolium intergeneric hybrid’s creation was carried out by embryo culture method from an immature caryopsis by growing on a nutrient medium. For the plant genotypes labeling the multilocus primers associated with coding DNA regions (Start Codon Targeted (SCoT) Polymorphism), SRAP (Sequence-related amplified polymorphism) have been selected. Results. The viable plants of intergeneric festulolium hybrids of Festuca arundinacea morphotype have been obtained. To select the best festulolium biotypes for variety populations with high feed and seed productivity formation. A system for hybrid plants genotypes and their parental forms registration in the form of molecular genetic passports have been elaborated. The genetic passports reflects the allele’s composition in loci associated with DNA coding sequences. Conclusions. The best biotypes with economically valuable traits were selected and included in the further selection process. The molecular genetic passports of hybrid festulolium plants and parental forms were composed.
Keywords: festulolium, immature caryopsis, biotypes, DNA, PCR, molecular genetic passports.
4
Petit, Thierry. "Monstres sauvages ou hybrides psychopompes ?" Dialogues d'histoire ancienne 43/1, no. 1 (2017): 13. http://dx.doi.org/10.3917/dha.431.0013.
Full text
5
Miller, D. Gary. "The Morphological Legacy of French." Diachronica 14, no. 2 (January 1, 1997): 233–64. http://dx.doi.org/10.1075/dia.14.2.03mil.
Full textAbstract:
SUMMARY A reexamination of a small portion of the morphological evidence reveals that there were no fewer than 100 hybrid derivatives (of the type French suffix on native base) prior to 1450 and at least 64 before 1400. Given that most of the texts are literary, those are fairly high numbers. Moreover, the more banal the hybrid, the more likely it was to be allowed to occur in literary texts. Not surprisingly, glossaries and other non-literary texts are rich in hybrids, implying that the application of French suffixes to non-French roots was not uncommon in colloquial ME. Bilingual selection initially yielded a treasury of diverse caiques and lexemes, but code-switching normally precluded hybrid formation. The reassertion of English was facilitated by greater convergence. Monolingual speakers of this contact language could not distinguish nativized French words from English, permitting overlapping domains and morphological transfer. RÉSUMÉ Un reexamen d'une partie limitee des evidences morphologiques revele qu'il n'y eut pas moins d'une centaine de derives hybrides (du type où un suffixe frangais se joint a une racine anglaise) anterieurs a 1450 et au moins 64 avant 1400. Etant donne que la plupart des attestations viennent de textes litteraires, ces chiffres correspondent a un taux assez eleve. D'ailleurs, plus Thybride etait banal, plus il etait apte a figurer dans des textes litteraires. On constate sans etonnement que les glossaires et les autres textes non-litteraires abondent en formations hybrides, ce qui implique que la jonction des suffixes frangais avec des racines anglaises ne fut pas rare en moyen-anglais popu-late. Au debut ce fut la selection bilingue qui produisit un tresor de lexemes divers et de caiques, tout en evitant des formations hybrides lesquelles, de toute maniere, ne sont pas typiques lorsqu'il y a alternance codique. C'est la convergence linguistique qui a ensuite facilite la resurgence de 1'anglais. Les locuteurs unilingues de cette langue de contact ne surent pas distinguer les formations frangaises naturalisees des formations anglaises, permettant ainsi le chevauchement des domaines et, par la, des transferts morphologiques. ZUSAMMENFASSUNG Eine frische Untersuchung eines kleinen Teilbereiches der morpholo-gischen Daten des Mittelenglischen zeigt, da6 vor 1450 nicht weniger als 100 Hybridisierungen existierten; vor 1400 lassen sich mindestens 64 belegen. Benicksichtigt man, daß die zugrundeliegenden Texte uberwiegend litera-risch sind, so sind diese Zahlen durchaus hoch. Daruberhinaus laBt sich beobachten, daB die Mischformen umso eher in literarischen Texten EinlaB fan-den, je banaler sie waren. Es uberrascht also nicht, daB Glossare und andere nichtliterarische Texte reichhaltig an Mischformen sind, was darauf schlieBen laBt, daB die Anheftung franzosischer Suffixe an nichtfranzosische Stamme im umgangssprachlichen Mittelenglisch nicht unublich war. Anfangs produ-zierte zweisprachige Selektion einen Reichtum von verschiedenen Lexemen und Lehniibersetzungen, Kodewechsel aber schloB typischerweise Hybridi-sierungen aus. Konvergenz erleichterte dann die Wiedererstarkung des Engli-schen. Einsprachige Sprecher dieser Kontaktsprache waren nicht in der Lage, vereinheimischte franzosische Worter von englischen auseinanderzuhalten, was Uberlagerungen und morphologischen Transfer erlaubte.
6
Belotserkovskii, Boris P., Gurucharan Reddy, and David A. Zarling. "DNA Hybrids Stabilized by Heterologies†." Biochemistry 38, no. 33 (August 1999): 10785–92. http://dx.doi.org/10.1021/bi990699p.
Full text
7
PIÉRARD, LAURENT, LIDA GARCÍA QUINTANA, MITCHELL E. REFF, and ALEX BOLLEN. "Production in Eukaryotic Cells and Characterization of Four Hybrids of Tissue-Type and Urokinase-Type Plasminogen Activators." DNA 8, no. 5 (June 1989): 321–28. http://dx.doi.org/10.1089/dna.1.1989.8.321.
Full text
8
Paull, Tanya T. "RNA–DNA hybrids and the convergence with DNA repair." Critical Reviews in Biochemistry and Molecular Biology 54, no. 4 (July 4, 2019): 371–84. http://dx.doi.org/10.1080/10409238.2019.1670131.
Full text
9
Wittung, Pemilla, Seog K. Kim, Ole Buchardt, Peter Nielsen, and Bengt Norden. "Interactions of DNA binding ligands with PNA-DNA hybrids." Nucleic Acids Research 22, no. 24 (1994): 5371–77. http://dx.doi.org/10.1093/nar/22.24.5371.
Full text
10
Kreike, C. M., J. R. A. de Koning, and F. A. Krens. "Non-radioactive detection of single-copy DNA-DNA hybrids." Plant Molecular Biology Reporter 8, no. 3 (August 1990): 172–79. http://dx.doi.org/10.1007/bf02669513.
Full textDissertations / Theses on the topic "DNA hybrides"
1
Cohen, Sarah. "Le rôle de senataxine dans la résolution des hybrides ARN : ADN aux cassures double brins de l'ADN." Thesis, Toulouse 3, 2019. http://www.theses.fr/2019TOU30125.
Full textAbstract:
Les gènes transcriptionellement actifs peuvent être la source de l'instabilité du génome via de nombreux mécanismes. Ces gènes sont caractérisés par la formation de structures secondaires telles que les hybrides ADN : ARN. Ils se forment lorsque l'ARN sortant l'ARN polymérase II s'hybride au simple brin d'ADN. De nombreuses études ont montrées que l'accumulation de ces hybrides peut mener à la création de dommages à l'ADN. Parmi ces dommages, les Cassures Double Brins (CDB) sont les plus dangereuses pour la cellule puisqu'elles peuvent produire des mutations et des réarrangements chromosomiques. Il existe deux mécanismes de réparation majeurs dans la cellule : la Jonction Non-Homologue des Extrémités (NHEJ) et la Recombinaison Homologue (HR). Mon équipe a récemment montré que les CDB localisées dans les gènes transcrits sont préférentiellement réparés par HR. De plus, de nombreuses études ont montrées une interaction entre transcription et réparation des CDB. Au vue de ces résultats, nous avons donc émis l'hypothèse que les gènes transcriptionellement actifs pourraient être réparés par un mécanisme spécifique nécessitant l'activité de protéines associées à la transcription : "Réparation couplée à la transcription". Durant ma thèse, je me suis intéressée au rôle de deux protéines dans la réparation des régions transcrites en utilisant la lignée cellulaire DIvA (DSB Induction via AsiSI) qui permet l'induction de cassures annotées sur tout le génome. Premièrement, nous avons montré que la réparation des CDB dans des loci transcrits nécessitent une hélicase ADN : ARN connue : sénataxine (SETX). Après induction d'une cassure dans un gène, SETX est recrutée ce qui permet la résolution d'hybride ADN : ARN (cartographié par DRIP-seq). Nous avons aussi montré que SETX permet le recrutement de RAD51 et limite les jonctions illégitimes des CDB et par conséquent promeut la survie des cellules après induction des cassures. Cette étude montre que les CDB dans les loci transcrits requièrent la résolution spécifique des hybrides ADN : ARN par SETX pour permettre une réparation précise et est absolument indispensable pour la survie cellulaire. Deuxièmement, nous avons montré une interaction entre SETX et Bloom (BLM) une G4 DNA hélicase dans la réparation des CDB dans les régions transcrites. Nous avons montré que BLM est aussi recrutée au CDB dans les loci transcrits où elle est nécessaire à la résection et à la fidélité de réparation. De façon importante, nous avons montré que la déplétion de BLM restaure le défaut de survie cellulaire observé dans les cellules déplétées pour SETX après induction des CDB. La déplétion d'autres hélicases G4 (RTEL1, FANCJ) promeut aussi la survie des cellules déplétées pour SETX après dommages. Ces résultats suggèrent une interaction entre les hélicases G4 et la résolution des hybrides ADN : ARN dans la réparation des gènes actifs. En conclusion, ces études permettent une meilleure compréhension de la spécificité de la réparation des régions transcrites du génome, et notamment l'identification de protéines impliquées dans la "Réparation couplée à la Transcription"Actively transcribed genes can be the source of genome instability through numerous mechanisms. Those genes are characterized by the formation of secondary structures such as RNA-DNA hybrids. They are formed when nascent RNA exiting RNA polymerase II hybridizes single stranded DNA. Numerous studies have shown that RNA-DNA hybrids accumulation can lead to DNA damages. Among those damages, DNA double strand breaks (DSB) are the most deleterious for cells since they can generate mutations and chromosomal rearrangements. Two major repair mechanisms exist in the cell: Non-Homologous End-Joining (NHEJ) and Homologous recombination (HR). My lab showed recently that DSB occurring in transcribed genes are preferentially repaired by HR. Moreover, multiple studies have shown a cross talk between transcription and DSB repair. Those results led us to propose that actively transcribed genes could be repaired by a specific mechanism implicating proteins associated with transcription: "Transcription-coupled DSB repair". During my PhD, using the DIvA (DSB Induction via AsiSI) cell line allowing the induction of annotated DSB through the genome, I worked on 2 projects focusing on DSB repair in transcribed genes. First, we showed that DSB repair in transcribed loci requires a known RNA: DNA helicase: senataxin (SETX). After DSB induction in an active gene, SETX is recruited which allows RNA-DNA hybrid resolution (mapped by DRIP-seq). We also showed that SETX activity allows RAD51 loading and limits DSB illegitimate rejoining and consequently promotes cell survival after DSB induction. This study shows that DSB in transcribed loci require specific RNA-DNA hybrids removal by SETX for accurate repair. Second, we showed an interplay between SETX and Bloom (BLM) a G4 DNA helicase in DSB repair induced in transcribed loci. We showed that BLM is also recruited at DSB in transcribed loci where it promotes resection and repair fidelity. Strikingly, we showed that BLM depletion rescued the survival defects observed in SETX depleted cells following DSB induction. Knock down of other G4-helicases (RTEL1, FANCJ) also promoted cell survival in SETX depleted cells upon damage. Those data suggest an interplay between G4 helicases and RNA: DNA resolution for DSB repair in active genes. Altogether, these studies promote a better understanding of the specificity of DSB repair in transcriptionally active genes, and notably identification of proteins involved in "Transcription-coupled DSB repair"
2
Mougeot, Romain. "Synthèse de sondes fluorescentes hybrides epicocconone-triphénylamine pour le piégeage de protéines liées aux zones à risques de l'ADN." Thesis, Normandie, 2018. http://www.theses.fr/2018NORMR126.
Full textAbstract:
La compréhension des mécanismes biologiques et l’implication des protéines dans ces mécanismes ont toujours été un enjeu important pour les biologistes. Les zones à risques de l’ADN impliquées dans les cancers, comme les G-quadruplex, les zones riches en Adénine-Thymine et leurs environnements proches sont particulièrement étudiés depuis de nombreuses années. L’essor des techniques d’analyses par fluorescence a permis aux scientifiques de mettre au point des sondes marquant ces domaines avec toujours plus de précision et de sensibilité. Cependant, de nombreuses interrogations existent sur la nature des interactions entre ces zones de l’ADN et les protéines. Afin de répondre à cette problématique, la synthèse d’une sonde pro-fluorescente alliant un ligand de l’ADN (conçu d’après les travaux des équipes de l’Institut Curie, UMR 176) à un piège à protéines (basé sur le squelette de l’epicocconone) a été réalisée et son efficacité biologique a été évaluée. Ces deux parties ont été assemblées en utilisant une réaction de cycloaddition 1,3 dipolaire spontanée entre un azoture et un alcyne contraint (SPAAC). De plus, au cours de ces travaux, une nouvelle bibliothèque de ligands de l’ADN a été synthétisée en utilisant une méthodologie innovante basée sur une réaction de C-H activation « on water »Understanding biological process and proteins involved in has challenged biologists’ mind for a while. Specific DNA sequences, such as G-quadruplex and Adenine-Thymine rich sequences, have been studied for many years, especially for their involvement in genetic diseases like cancer. Scientists have also been interested in fluorescence monitoring and imaging of these specific sequences for a long time. Indeed, the huge sensitivity of these fluorescent technics and the wide scope of synthetic dyes available allowed several improvements on targeting DNA sequences responsible for genetic disorders. Nonetheless, relation between proteins and these areas remains mostly unknown. In order to answer this question, a pro-fluorescent dye built of two main parts, which are a DNA ligand (designed by Curie Institute teams, UMR 176) and a protein trap (based on epicocconone core). These parts were synthesized, coupled thanks to a Spontaneous Azoture Alkyne Cycloaddition (SPAAC) and the biological properties of the probe were evaluated. Furthermore, new ligands were synthesized using a new and innovating method of “on water” C-H activation reaction
3
Liu, Yaqun. "Study of transcription-replication conflict and its role in genomic instability and cancer development." Electronic Thesis or Diss., Université Paris sciences et lettres, 2022. http://www.theses.fr/2022UPSLS083.
Full textAbstract:
Les machineries de réplication et de transcription peuvent provoquer des conflits entre transcription et réplication (TRC), qui se produisent de manière frontale ou co-directionnelle. La collision frontale est considérée comme étant la plus délétère et peut conduire à de l’instabilité génomique au travers des R-loops qui se composent un hybride ADN-ARN et un brin d'ADN déplacé. En analysant les données multi-omiques, nous avons révélé avec succès que la pause transitoire de la fourche de réplication aux 3' des gènes enrichis en R-loops avec collision frontale affecte la stabilité génomique d'une manière dépendante de Topoisomérase1 (Nat.Communs. 2020) puis j'ai développé le premier outil bio-informatique pour analyser de données de réplication (OKseqHMM, disponible sur GitHub, Liu et al. BioRxiv. 2022). Finalement, il a été montré récemment que dans les cellules cancéreuses du sein, les R-loops colocalisent fortement avec une augmentation des cassures de l'ADN, de manière dépendante de la réplication. Nous visons à étudier le TRC dans des cellules cancéreuses et des échantillons de patients cancéreux pour déterminer comment le stress réplicatif induit de l'instabilité génomique dans le développent de cancer, ce qui pourront contribuer à l’établissement de nouvelles stratégies thérapeutiques contre le cancerReplication and transcription machinery can cause transcription-replication conflicts (TRCs), which occur either frontally or co-directionally. The head-on collision is considered to be the most deleterious and can lead to genomic instability through R-loops that consist of a DNA-RNA hybrid and a displaced DNA strand. By analyzing multi-omics data, we successfully revealed that transient replication forks pause at the 3' of genes enriched in R-loops with more head-on collisions affects genomic stability in a Topoisomerase1-dependent manner (Nat. Commons . 2020) then I developed the first bioinformatics tool to analyze replication data (OKseqHMM, available on GitHub, Liu et al. BioRxiv. 2022). Finally, it has recently been shown that in breast cancer cells, R-loops strongly colocalize with an increase in DNA breaks, in a replication-dependent manner. We aim to study TRC in cancer cells and samples from cancer patients to determine how replicative stress induces genomic instability in cancer development, which may contribute to the establishment of new therapeutic strategies against cancer
4
Le, ho Khanh hy. "Synthèse par « Click Chemistry » de matériaux hybrides et éudes de leurs assemblages supramoléculaires." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA112285/document.
Full textAbstract:
L’approche « bottum-up » via l’auto-assemblage moléculaire est considéré comme une voie prometteuse pour contrôler la fabrication de nouveaux matériaux et leur intégration dans des dispositifs hybrides présentant de propriétés nouvelles. Dans ce travail, nous avons synthétisé plusieurs hybrides à base de molécules organiques (fullerène, porphyrines, phtalocyanine), d’oligonucléotides ou de nanotubes de carbone.Dans un premier temps, nous nous sommes intéressés à la synthèse d’une nouvelle famille de produits constituée d’une unité C60 lié à deux chromophores positionnés face à face et permettant la formation de complexes hôte-invités. Nous avons montré que ces composés s’assemblent pour donner des structures supramoléculaires en solution et sur surface. Les interactions électroniques et la compléxation entre le fullerène et les deux chromophores (porphyrines et phtalocyanines) ont été étudiées par spectroscopie optique et RMN ainsi que par voltammétrie cyclique.Parmi les outils de l’approche « bottom-up », l’ADN a montré son extraordinaire potentiel pour la fabrication d’assemblages bio-dirigés. En effet, la synthèse de matériaux hybrides à base d’ADN permet un contrôle précis (théoriquement à l’échelle d’une base, ~3,4Å) du positionnement des groupements fonctionnels dans un matériau. Dans le but de former des réseaux bi- et tridimensionnels à base d’ADN permettant le positionnement de nano-objets, nous avons synthétisé des hybrides à base d’oligonucléotides et de porphyrines (molécule 2D) ou d’adamantane (molécule 3D). Des édifices supramoléculaires simples ont été réalisés et le travail se poursuit en vue de la réalisation de réseaux fonctionnels.Enfin, dans une dernière partie, nous nous sommes intéressés à la fonctionnalisation des nanotubes de carbone monoparoi (SWNT) avec des chromophores de type porphyrines et phtalocyanines. Alors que les porphyrines présentent une absorption intense presque exclusivement dans le bleu, les phtalocyanines absorbent principalement dans le rouge. Combiner ces deux chromophores à la surface des nanotubes de carbone présente donc un intérêt particulier pour la collecte de lumière car les deux composés absorbent des régions complémentaires du spectre visible. Ce travail ouvre la voie vers l'étude des propriétés optoélectroniques des hybrides à base de nanotubes et en particulier leur utilisation pour la conversion d’énergie lumineuse en énergie électrique (application photovoltaïque)An Approach "bottum-up" via molecular self-assembly is considered as a promising way to control the manufacture of new materials and their integration into hybrid devices with novel properties. In this work, we have synthesized several hybrids based on organic molecules (fullerene, porphyrin, phthalocyanine), oligonucleotides or carbon nanotubes.At first, we were interested in the synthesis of a new family of products consisting of a unit C60 linked to two chromophores positioned face to face and allowing the formation of host-guest complexes. We have shown that these compounds are combined to give supramolecular structures in solution and on the surface. Electronic interactions and complexation between fullerene and the two chromophores (porphyrins and phthalocyanines) were studied by NMR and optical spectroscopy as well as cyclic voltammetry.Among the tools of the "bottom-up", DNA showed its tremendous potential for the production of bio-directed assembly. Indeed, the synthesis of hybrid materials based DNA allows precise control (theoretically on the scale of a base, ~ 3.4 Å) of the positioning of the functional groups in a material. In order to form networks and bi-dimensional DNA-based for positioning nano-objects, we have synthesized hybrid oligonucleotide-based and porphyrin molecule (2D) or adamantane molecule (3D). Supramolecular structures have been made and this work is ongoing to achieve functional networks.Finally, in a last part, we are interested in the functionalization of single-walled carbon nanotubes (SWNTs) with chromophores like porphyrins and phthalocyanines. While porphyrins exhibit almost exclusively an intense absorption in the blue (around 420-440 nm), phtalocyanines absorb mainly in the red spectral region. Taken together these two chromophores have interesting light harvesting, photophysical and redox properties; the two components will participate independently to increase the overall absorption in the visible range of the solar spectrum. This work opens the route to study the optoelectronic properties of hybrid nanotube and in particular their use for the conversion of light energy into electrical energy (photovoltaic application)
5
Rigal, Mélanie. "Etude de la stabilité de la méthylation ADN chez Arabidopsis thaliana et impact sur la transcription." Thesis, Clermont-Ferrand 2, 2014. http://www.theses.fr/2014CLF22496/document.
Full textAbstract:
La maintenance de la méthylation ADN sur les sites CG joue un rôle crucial dans le silencing des éléments transposables (TE) et l’expression correcte des gènes. Chez Arabidopsis thaliana, on observe, dans le mutant met1-3 déficient en méthylation CG, une apparition ectopique de méthylation CHG sur de nombreux gènes, ainsi qu’une relocalisation de la diméthylation de la lysine 9 de l’histone H3 (H3K9me2) de l’hétérochromatine vers l’euchromatine. Nous avons démontré que ceci est lié à un défaut de transcription au niveau du grand intron du gène codant la déméthylase H3K9me2 IBM1. Nous avons également constaté que dans les épihybrides F1 issus du croisement de plantes met1-3 avec une plante sauvage la méthylation CHG présente dans l’intron de l’allèle IBM1 hérité du parent mutant était perdue. Afin de définir si la perte de méthylation affecte également d’autres loci génomiques, et plus globalement à l’échelle du génome entier l’impact de la rencontre de deux épigénomes différents, nous avons analysé les profils de méthylation, de siRNAs et de transcription des épihybrides F1. Nos données révèlent que l’union de deux méthylomes distincts au sein d’un même génome provoque une restructuration considérable des profils épigénétiques et transcriptionnels. La méthylation CHG apparaissant sur de nombreux gènes dans met1 tend à persister, créant ainsi de nouveaux épiallèles pouvant être hérités. Du côté des TE, nombre d’entre eux sont déméthylés et réactivés, tandis que d’autres sont immédiatement reméthylés et resilencés. Ainsi, ces résultats contribuent à la compréhension de la stabilité de la méthylation ADN et de son rôle dans le contrôle différentiel des gènes et des TEMaintenance of DNA methylation at CG sites is crucial for silencing of transposable element (TE) and proper expression of genes. In Arabidopsis thaliana, met1-3 mutant, deficient in CG methylation, shows ectopic appearance of CHG methylation at numerous genes, as well as relocation of H3K9me2, from heterochromatin towards euchromatin. We have shown that this is due to a defect of the transcription of the large intron of the gene encoding the IBM1 H3K9me2 demethylase. We also found that, in the F1 epihybrids from the cross between met1 and WT plants, CHG methylation at the intron of the met1-derived IBM1 allele is lost. In order to define whether the loss of methylation at IBM1 also affects other genomic loci, and the impact of the union of two different epigenomes on methylation and transcription genome-wide, we analyzed the methylation, siRNA and transcription patterns of F1 epihybrids. Our data reveal that the union of two distinct methylomes within the same genome triggers considerable restructuring of epigenetic and transcriptional patterns. CHG methylation appearing in the mutant parent tends to persist in F1, creating new epialleles that can be inherited. On the TE side, lots of them are demethylated and reactivated while others are immediately remethylated and resilenced. Thus, our results provide new insights to the understanding of DNA methylation stability and its role in the differential control of genes and TEs
6
Bertucci, Alessandro. "Hybrid organic-inorganic interfaces for biomedical applications." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF008/document.
Full textAbstract:
Le travail de recherche de cette thèse consiste en le développement de nouveaux matériaux hybrides organiques-inorganiques pour des applications en nanotechnologie, nanomédicine et diagnostic. Dans ce contexte, des cristaux poreux de zéolite-L ont été utilisé comme nano-vecteur pour faire de la transfection d’ADN et d’ANP, en combinaison avec le relargage de molécules hôtes placées dans les pores. Des nanoparticules de silice mesoporeuses multifonctionnelles ont été utilisées pour traiter le glioblastome, en combinant la thérapie génique avec l’administration durable d’un principe actif. Des nano-coquilles hybrides biodégradables ont été encore développés pour encapsuler des protéines et les relâcher dans les cellules vivantes. Dans le domaine de la détection d’acides nucléiques, des fibres optiques à cristal photonique, fonctionnalisées avec des sondes d’ANP, ont été exploitées comme plateformes optiques pour faire de la détection ultra-sensible d’oligonucléotides ou d’ADN génomique. Enfin, la squelette de l’ANP a été modifié à créer des sondes fluorescentes pour reconnaître et détecter la présence des séquences cibles spécifiquesThe research work presented throughout this thesis focuses on the development of novel organic-inorganichybrid materials for applications in nanotechnology, nanomedicine and diagnostics. In such a context, porous zeolite-L crystals have been used as nanocarriers to deliver either DNA or PNA in live cells, in combination with the release of guest molecules placed into the pores. Multifunctional mesoporous silica nanoparticles have been designed to treat glioblastoma, combining gene therapy with the sustained delivery of a chemotherapy agent. Biodegradable hybrid nano-shells have been furthermore created to encapsulate proteins and release them in living cells upon degradation of the outer structure in reductive environment. In the field of nucleic acid detection, photonic crystal fibers, functionalized with specific PNA probes, have been exploited as optical sensing devices to perform ultra-sensitive detection of DNA oligonucleotides or genomic DNA. Eventually, the PNA backbone has served as scaffold to synthesize fluorescent switching probes able to recognize and to detect the presence of specific target sequences
7
Amirbekyan, Karen. "Etude de l'interaction des nouveaux dérivés de Hoechst 33258 avec l'ADN et d’induction d’excimères en présence d’ADN de différentes sondes pyrénylées." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT193.
Full textAbstract:
Le développement de nouvelles molécules capables d’intéragir avec l’ADN, leur mode d’intéraction et leur affinité est un domaine de recherche particulièrement important. Dans ce travail nous avons étudié les interactions avec l’ADN de la molécule Hoechst 33258 connu pour être un ligand du petit sillon ainsi que plusieurs de ces analogues.Dans un premier temps nous avons étudié la stabilité des complexes ADN-Hoechst 33258 en solution avec et sans DMSO comme co-solvant. Deuxièmement, les affinités de dérivés nouvellement conçus et synthétisés du Hoechst 33258 vis-à-vis de l'ADN ont été évaluées. Enfin, nous avons étudiés la capacité d’induction d’excimers en présence d’ADN de différentes molécules pyrénylées. Ces études ont été effectuées par différentes méthodes spectroscopiques, telles que l'absorbance UV-visible, la fluorescence, le dichroïsme circulaire, la spectroscopie de masse ESI et de la modélisation moléculaireThe development of new DNA binders and the evaluation of their affinity toward DNA as well as their mode of binding is an area of research of prime importance. In this thesis we studied the interactions of Hoechst 33258, a well-known groove binder, as well as some of its newly synthesized derivatives with DNA. The stability of DNA-Hoechst 33258 complex in solution with and without DMSO as a co-solvent was evaluated.Secondly, the affinities of newly designed and synthesized derivatives of Hoechst 33258 toward DNA were evaluated. Finally, a set of pyrene derivatives able to induced excimer formation upon binding to DNA were studied. Different spectroscopic methods, such as UV-vis absorbance, fluorescence, circular dichroism, ESI mass spectroscopy and molecular docking were applied for the complete evaluation of the affinity of these ligands toward DNA
8
Kemiha, Samira. "Étude du rôle des protéines Ribonucléases H dans la réponse cellulaire au stress réplicatif." Electronic Thesis or Diss., Université de Montpellier (2022-....), 2022. http://www.theses.fr/2022UMONT020.
Full textAbstract:
Au cours de la phase S, la réplication de l’ADN est initiée au niveau de multiples origines réparties le long du génome. La machinerie de réplication, ou réplisome, peut rencontrer des obstacles ralentissant sa progression, comme des structures secondaires de l’ADN ou des protéines liées à l’ADN telles que les ARN polymérases, générant ainsi ce que l’on appelle un stress réplicatif. Les réplisomes bloqués par un obstacle sont des structures fragiles qui peuvent générer des cassures et conduire à l’instabilité du génome. Lorsque la progression de l’ARN polymérase est ralentie ou arrêtée, le brin d’ARN naissant peut potentiellement s’hybrider avec le brin d’ADN complémentaire en déplaçant le second brin d’ADN, formant ainsi une structure à trois brins appelée R-loop, pouvant entraver la progression du réplisome. La coordination des processus de réplication et de transcription limite les interférences entre la réplication et la transcription. Cependant, cette coordination n’est pas parfaite et même dans des conditions physiologiques, la transcription et l’accumulation de R-loops peuvent conduire à des évènements de recombinaison, notamment au cours de la phase S. Les Ribonucléases H (RNases H) de type 1 et 2 sont des protéines impliquées dans la résolution des R-loops par la dégradation spécifique du brin d’ARN au sein du duplexe ARN:ADN. Les cellules dépourvues de RNases H présentent une accumulation de R-loops et sont extrêmement sensibles à différents agents génotoxiques induisant du stress réplicatif (e.g. MMS : méthanesulfonate de méthyle ou HU : hydroxyurée). Le but de mes travaux de thèse est de déterminer le rôle des RNases H dans la réponse cellulaire au stress réplicatif. Dans deux modèles cellulaires, la levure S. cerevisiae et les cellules humaines, nous avons pu montrer que les cellules déplétées en RNases H présentent des défauts de prise en charge et de redémarrage des fourches de réplication arrêtées en condition de stress réplicatif induit. L'utilisation de mutants de séparation de fonction de RNase H2 suggère que l’élimination défectueuse des hybrides ARN:ADN est responsable de ces défauts. La mesure du taux d’hybrides ARN:ADN au cours du cycle cellulaire montre qu’il augmente en phase S en présence de stress réplicatif exogène dans des cellules sauvages et mutantes pour RNases H. De plus, nos résultats indiquent que l’inhibition de la transcription ou la surexpression de l’hélicase ARN:ADN Sénataxine restaure la prise en charge et le redémarrage des fourches de réplication arrêtées lors d’un stress induit par le MMS et en absence des RNases H. Ainsi, l’ensemble de nos résultats suggère une étroite coopération entre les Ribonucléases H et l’hélicase Sénataxine pour résoudre les interférences entre les ARN polymérases et/ou les hybrides ARN:ADN avec les machineries de réplicationDuring S phase, DNA replication starts at multiple origins distributed throughout the genome. As the replication machinery (or replisome) progresses throughout the DNA, it often encounters obstacles such as DNA secondary structures or transcription complexes, thereby generating what is called replication stress. Stalled replisomes are fragile structures that can give rise to chromosome breaks and trigger genome instability. When RNA polymerases stall, the nascent RNA can potentially anneal with the template DNA strand, creating a three-strand structure called R-loop. Coordination between replication and transcription in S phase limits the risks of collisions between the replisome and RNA polymerases. Even though, physiological transcription level and R-loops accumulation lead to recombination events in S phase. Type 1 and 2 ribonucleases H (RNase H) are specific proteins involved R-loops’ resolution through the degradation of the RNA strand within the RNA:DNA duplex. In the absence of RNases H, cells accumulate R-loops and are extremely sensitive to different replication stress-inducing genotoxic agents (e.g. MMS: methyl methanesulfonate or HU: hydroxyurea).The goal of my PhD project was to assess the roles of RNases H in the cellular response to replication stress. Using two cellular models, the budding yeast S. cerevisiae and mammalian cells, we demonstrated that RNases H mutations induce HU- and MMS-stalled replication forks processing and restart defects. Analysis of separation-of-function RNase H2 mutants suggests that it is the RNA:DNA hybrids removal activity of RNase H2 that is important for the correct processing of stalled forks experiencing replication stress. Indeed, quantification of RNA:DNA hybrids during the cell cycle reveals a higher level of hybrids in S phase in the presence of exogenous replication stress in both wild-type and RNases H-depleted cells. Moreover, our results demonstrate that the inhibition of transcrip tion or the overexpression of the RNA:DNA helicase Senataxin restore stalled replication fork processing and restart upon MMS treatment when cells lack RNase H2 activities. Altogether, our data indicate that Ribonucleases H1 and 2 and Senataxin helicase cooperate to resolve RNA polymerases and/or RNA:DNA hybrids interferences with replication
9
Castro, Smirnov Fidel Antonio. "Physicochemical characterization of DNA-based bionanocomposites using nonafibrous clay minerals : biological applications." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112260/document.
Full textAbstract:
Parmi les différents minéraux argileux, la sépiolite, qui est un silicate fibreux naturel, est un potentiel nano-transporteur prometteur pour le transfert non-viral de biomolécules. Il a en effet été montré que la sépiolite interagissait avec des molécules biologiques telles que les lipides, les polysaccharides et les protéines. Dans ce travail, nous démontrons que la sépiolite interagit également efficacement avec différents types de molécules d'ADN (génomique, plasmidique, oligonucléotides simple et double brin), et nous présentons la première étude détaillée sur les mécanismes d'interaction entre la sépiolite et l'ADN, ainsi qu’une caractérisation physico-chimique de bionanocomposites ADN-sepiolite. Une analyse spectroscopique a montré tout d’abord que l’interaction de l'ADN avec la sépiolite était plus forte en présence de polycations, la valence de ces derniers accroissant le rendement d’absorption, et deuxièmement, que l'ADN ainsi adsorbé pouvait être récupéré avec un rendement modulé par la présence d’EDTA, la structure de l'ADN et son activité biologique étant conservées. Par spectroscopie infrarouge à transformée de Fourier (FTIR) nous avons identifié les groupes silanol externes comme les principaux sites d'interaction avec l'ADN. Nous avons ensuite prouvé qu'il est possible d'utiliser la sépiolite pour extraire l'ADN de bactéries, pour la purification de l'ADN et pour la purification de toute contamination bactérienne. En combinant la microscopie à fluorescence, la microscopie électronique à transmission (MET), la vidéo-microscopie et l’analyse par cytométrie en flux (FACS), nous avons montré que la sépiolite peut être spontanément internalisée dans des cellules de mammifère par le biais de deux voies, l’endocytose et la macropinocytose. En tant que preuve de concept, nous montrons que la sépiolite est capable de transférer de manière stable l'ADN de plasmide dans des bactéries et des cellules de mammifères. Il a également été prouvé qu’en incubant des bactéries avec des bionanocomposites ADN-sepiolite, initialement préparés en présence d'une faible concentration en cations divalents et avec de la sépiolite traitée aux ultrasons (sSep), il était possible d'augmenter l'efficacité de la transformation bactérienne 20 à 30 fois par rapport aux méthodes basées sur l'«effet Yoshida». En outre, nous montrons que l'efficacité du transfert de gènes par la sépiolite peut être optimisée : l'utilisation de sSep et l'exposition à la chloroquine augmentent d’un facteur 100 et 2, respectivement, l’efficacité de transfection. Ces résultats ouvrent la voie à l'utilisation de bionanocomposites à base de sépiolite comme de nouveaux potentiels nano-transporteurs hybrides potentiels, à la fois pour la thérapie génique et le développement de nouveaux modèles biologiques en sciences fondamentales et appliquéesAmong the various clay minerals, sepiolite, which is a natural fibrous silicate, isa potential promising nanocarrier for the non-viral transfer of bio-molecules. Indeed,sepiolite has been shown to interact with biological molecules such as lipids,polysaccharides and proteins. Here, we show that sepiolite efficiently binds differenttypes of DNA molecules (genomic, plasmid, single strand and double strandoligonucleotides), introducing the first detailed study on the interaction mechanismsbetween sepiolite and DNA, as well as the physicochemical characterization of theresulting DNA-sepiolite bionanocomposites. The interaction mechanisms aresuggested to be electrostatic interactions, van der Waals forces, cation bridges, andhydrogen bonding. Spectroscopy analysis showed that the binding of DNA to sepiolitewas increased by polycations with valence dependent efficiency, and the DNApreviously adsorbed could be recovered with an efficiency that could be modulatedusing a chelating agent (EDTA), preserving the DNA structure and biological activity.Fourier-transform infrared spectroscopy identified the external silanol groups as themain sites of interaction with the DNA. It was proved that it is possible to use sepiolitefor extracting DNA from bacteria, for DNA purification and for purification from bacterialcontamination. By combining fluorescence microscopy, transmission electronmicroscopy (TEM), time-lapse video microscopy and flow cytometry analysis (FACS),we show that sepiolite can be spontaneously internalized into mammalian cells throughboth endocytic and non-endocytic pathways. As a proof of concept, we show thatsepiolite is able to stably transfer plasmid DNA into bacteria and mammalian cells. Itwas also proved that with the incubation of bacteria with the Sep/DNAbionanocomposite initially prepared in the presence of a low concentration of divalentcation, and using sonicated sepiolite (sSep), it is possible to increase the bacterialtransformation efficiency from 20 to 30-fold compared to previously reported methodswhich are based in the “Yoshida effect”. Additionally, we show that the efficiency ofsepiolite-mediated gene transfer can be optimized: the use of sSep and the exposureto the endosome disrupter chloroquine 100-fold and 2-fold stimulated DNA transfectionefficiency, respectively. These results open the way to the use of sepiolite-basedbionanocomposites as a novel class of hybrid nanocarriers for both potential genetherapy and the development of novel biological models of interest for academic andapplied sciences
10
Diallo, Amy. "The DNA translocation apparatus involved in Streptococcus Pneumoniae transformation." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066334/document.
Full textAbstract:
La transformation naturelle bactérienne permet aux micro-organismes d'échanger des informations génétiques pour promouvoir leurs réponses adaptatives pour faire face aux changements environnementaux. De l'ADN extracellulaire est incorporé et recombiné au génome de l'hôte. Ce processus augmente la plasticité des bactéries. Chez S. pneumoniae, un pathogène majeur chez l'Homme engendrant des infections pouvant être mortelles, la transformation bactérienne accentue la transmission de gènes de résistance aux antibiotiques. Chez les bactéries à Gram positif, l'opéron comF encode l'expression de deux protéines. L'une est démontrée comme étant essentielle à la transformation, est décrite pour être membranaire. La seconde n'a pas été étudiée. Cependant ces protéines n'ont pas été étudiées d'un point de vue structural ou fonctionnel. Des mutagenèse et le double hybride bactérien ont permis de mettre en évidence que ses protéines sont indispensables pour l'expression de la compétence et interagissent avec de nombreuses protéines du transformasome. De plus, l'expression des deux protéines de manière hétérologue prouve qu'elles sont solubles et forment des oligomères. L'analyse structurale de ComFA, atteste de la conformation atypique de cette helicase trimerique et hexamerique. En outre, l'activité ATPasique simple brin DNA-dépendant de cette protéine est démontrée. Finalement un complexe protéique a été révélé entre ComFA et ComFC dont l'étude microscopique à hautes résolutions prouve l'apparition d'un anneau via l'assemblage de deux hexamères. Ces résultats suggèrent que ComFA est le moteur tirant l'ADN dans la cellule. Quant à ComFC, elle semble aider à la stabilisation de ComFABacterial natural transformation allows microorganisms to exchange genetic information to promote their adaptive responses to cope with environmental changes. The extracellular DNA is incorporated and recombined with the genome of the host. This phenomenon increases the plasticity of Gram positive and negative bacteria. S. pneumoniae is a major pathogen for humans, which is causing infections that can be deadly. In this specie, bacterial transformation increases the transmission of antibiotic resistance.In Gram-positive bacteria, comF operon encodes the expression of two proteins. One of them, shown to be essential for natural transformation, is expected to be a membrane protein. The second is not described. However, up to now neither protein has been studied from a structural or functional point of view. Mutagenesis technique and double hybrid bacterial assay allowed to show that both proteins are essential for the expression of the competence and interact with many proteins of the transformasome. In addition, heterologous expresion of both proteins have shown their solubility and the formation of oligomers. Structural analysis of ComFA demonstrates the unique conformation of this hexameric and trimeric helicase. Furthermore, the ATPase single stranded DNA-dependent activity of this protein could be detected. Finally, a protein complex is formed between ComFA and ComF, and high-resolution microscopic study proves the occurrence of a ring via a two-hexamers. These results suggest that ComFA is the engine pulling the DNA in the cell. As for ComFC, this protein seems to help stabilizing of ComFA
Books on the topic "DNA hybrides"
1
Za jiao yu mi pin zhong DNA zhi wen tu pu: DNA finger print of maize hybrids. Beijing Shi: Zhongguo nong ye ke xue ji shu chu ban she, 2004.
Find full text
2
Kingsbury, Noël. Hybrid. Chicago: University of Chicago Press, 2009.
Find full text
3
Banasik, Mirosław. Wojna hybrydowa i jej konsekwencje dla bezpieczeństwa euroatlantyckiego. Warszawa: Wydawnictwo "Difin", 2018.
Find full text
4
Hybrid: The history and science of plant breeding. Chicago: The University of Chicago Press, 2009.
Find full text
5
Grochocka, Julia Anna. Wojna hybrydowa na Ukrainie: Wnioski i rekomendacje dla Europy i świata. Piotrków Trybunalski: Wydawnictwo Uniwersytetu Jana Kochanowskiego Filia w Piotrkowie Trybunalskim, 2017.
Find full text
6
Arrabito, Giuseppe, and Liqian Wang. DNA Nanotechnology for Bioanalysis: From Hybrid DNA Nanostructures to Functional Devices. World Scientific Publishing Co Pte Ltd, 2017.
Find full text
7
Adolph, Kenneth W. Methods in Molecular Genetics: Human Molecular Genetics (Methods in Molecular Genetics). Academic Pr, 1996.
Find full text
8
Methods in Molecular Genetics: Gene and Chromosome Analysis, Part A (Methods in Molecular Genetics). Academic Press, 1993.
Find full text
9
Adolph, Kenneth W. Methods in Molecular Genetics: Gene and Chromosome Analysis, Parts A, B, and C (Methods in Molecular Genetics). Academic Press, 1994.
Find full text
10
Adolph, Kenneth W. Methods in Molecular Genetics: Human Molecular Genetics (Methods in Molecular Genetics). Academic Pr, 1996.
Find full textBook chapters on the topic "DNA hybrides"
1
Margenstern, Maurice, Victor Mitrana, and Mario J. Pérez-Jiménez. "Accepting Hybrid Networks of Evolutionary Processors." In DNA Computing, 235–46. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11493785_21.
Full text
2
Wen, Meilin. "Hybrid DEA." In Uncertain Data Envelopment Analysis, 139–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-43802-2_6.
Full text
3
Blum, Christian, and Mateu Yábar Vallès. "Multi-level Ant Colony Optimization for DNA Sequencing by Hybridization." In Hybrid Metaheuristics, 94–109. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/11890584_8.
Full text
4
Cao, Yuanyuan, and Shunai Che. "DNA Condensed Phase and DNA-Inorganic Hybrid Mesostructured Materials." In ACS Symposium Series, 49–79. Washington, DC: American Chemical Society, 2017. http://dx.doi.org/10.1021/bk-2017-1252.ch004.
Full text
5
Bock, Christoph, Luca Bortolussi, Thilo Krüger, Linar Mikeev, and Verena Wolf. "Model-Based Whole-Genome Analysis of DNA Methylation Fidelity." In Hybrid Systems Biology, 141–55. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-26916-0_8.
Full text
6
Schwenger, Alexander, Helmut Griesser, and Clemens Richert. "DNA-Based Nanostructuring with Branched Oligonucleotide Hybrids." In DNA in Supramolecular Chemistry and Nanotechnology, 375–96. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781118696880.ch5.3.
Full text
7
Earle, Elizabeth D. "Mitochondrial DNA in Somatic Hybrids and Cybrids." In The molecular biology of plant mitochondria, 557–84. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0163-9_17.
Full text
8
García-Rubio, María, Sonia I. Barroso, and Andrés Aguilera. "Detection of DNA-RNA Hybrids In Vivo." In Methods in Molecular Biology, 347–61. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7306-4_24.
Full text
9
Jackson, J. F. "DNA Repair in Petunia hybrida Pollen." In Sexual Reproduction in Higher Plants, 81–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73271-3_13.
Full text
10
Kyriakopoulos, Charalampos, Pascal Giehr, Alexander Lück, Jörn Walter, and Verena Wolf. "A Hybrid HMM Approach for the Dynamics of DNA Methylation." In Hybrid Systems Biology, 117–31. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-28042-0_8.
Full textConference papers on the topic "DNA hybrides"
1
Weck, Johann M., Lea M. Wassermann, and Amelie Heuer-Jungemann. "DNA origami and DNA origami silica hybrids for biomedical applications." In Colloidal Nanoparticles for Biomedical Applications XVI, edited by Marek Osiński and Antonios G. Kanaras. SPIE, 2021. http://dx.doi.org/10.1117/12.2578358.
Full text
2
Badyanov, E. V., and S. A. Ramazanova. "THE TESTING OF SSR MARKERS OF GENES CONTROLLING RESISTANCE TO P. HALSTEDII AND THE SELECTION OF OPTIMAL CONDITIONS FOR PCR." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-19-24.
Full textAbstract:
Downy mildew is one of the most harmful diseases of sunflower. The most effective measure of this disease control is the development of resistant varieties and hybrids. The use of molecular markers, in particular DNA markers, allows to control the presence of dominant resistance genes at each stage of breeding. We carried out the selection of the optimal method for the isolation of sunflower DNA and the selection of the optimal temperature regimes of amplification for 10 pairs of primers developed to mark the Pl6, Pl8, Pl13, and PlArg genes. Preliminary, we identified 13 allelic variants that are suitable for DNA genotyping of Helianthus annuus by these loci.
3
Lin, Yueh-Cheng, Chi-Hsien Cheng, and Yu-Chueh Hung. "Stimulus pulse-dependent responses in natural DNA biopolymer devices." In Organic and Hybrid Sensors and Bioelectronics XIV, edited by Ruth Shinar, Ioannis Kymissis, and Emil J. List-Kratochvil. SPIE, 2021. http://dx.doi.org/10.1117/12.2593450.
Full text
4
Han, Aili. "RLM: A New Method of Encoding Weights in DNA Strands." In 2006 Sixth International Conference on Hybrid Intelligent Systems. IEEE, 2006. http://dx.doi.org/10.1109/his.2006.264900.
Full text
5
Kawabe, Yutaka. "Incorporation of photo-controllable molecules in tunable DNA dye laser system." In Organic and Hybrid Sensors and Bioelectronics XI, edited by Ruth Shinar, Ioannis Kymissis, Luisa Torsi, and Emil J. List-Kratochvil. SPIE, 2018. http://dx.doi.org/10.1117/12.2320947.
Full text
6
Norwood, R. A., J. Thomas, N. Peyghambarian, J. Wang, L. Li, F. Ouchen, and J. E. Grote. "Hybrid DNA materials for energy storage." In SPIE NanoScience + Engineering, edited by Norihisa Kobayashi, Fahima Ouchen, and Ileana Rau. SPIE, 2010. http://dx.doi.org/10.1117/12.862412.
Full text
7
Ogata, Naoya, Yoshiharu Kagami, Masahiro Wada, and Junichi Yoshida. "DNA-hybrid materials for photonic applications." In NanoScience + Engineering, edited by Emily M. Heckman, Thokchom B. Singh, and Junichi Yoshida. SPIE, 2007. http://dx.doi.org/10.1117/12.742131.
Full text
8
Patel, Rashmit, and Rutu Parekh. "DNA Based Hybrid Circuit Design Approaches." In 2019 IEEE 5th International Conference for Convergence in Technology (I2CT). IEEE, 2019. http://dx.doi.org/10.1109/i2ct45611.2019.9034066.
Full text
9
Han, Aili. "DNA Computing Model for the 0/1 Knapsack Problem." In 2006 Sixth International Conference on Hybrid Intelligent Systems (HIS'06). IEEE, 2006. http://dx.doi.org/10.1109/his.2006.264901.
Full text
10
Ismael, Yaseen. "Secure Image Steganography by Utilizing DNA Properties." In 3rd International Conference of Mathematics and its Applications. Salahaddin University-Erbil, 2020. http://dx.doi.org/10.31972/ticma22.08.
Full textAbstract:
In the last period, Steganography is commonly used as an alternative to encryption to achieve secret communication between parties. Many methods have emerged to achieve steganography, including the use of spatial domain, spread spectrum, transform domain, and etc. On the other hand, the methods of attackers have also developed in revealing hidden data and trying to retrieve it. To increase the security of the hiding process, some researchers have found hybrid methods that combine encryption and steganography processes. The research aims to present a new method in steganography by taking advantage of the properties of DNA, which includes the random sequence of nitrogenous bases (A, C, G, T), the process of hybridization, which occurs between two single strands of DNA to form a double strand of DNA so that the bases in the first strand are complementary to the nitrogenous bases in the second strand. The research includes the following steps: First, the secret image to be hidden is encrypted by encoding it into a series of nitrogenous bases, and then the XOR process is performed with a nitrogenous bases sequence for a DNA tape agreed upon between the sender and recipient, the hybridization process applied before and after the XOR process. The results show that encrypted image is much different from the original image and thus they added another level of security to the hidden image. Secondly, the encrypted image resulting from the first step is hidden in the cover image and using a new method based on the use of the agreed-upon DNA tape as a key.
Reports on the topic "DNA hybrides"
1
Schikora, B., S. Hietala, L. Shi, L. Lee, E. Skowronski, and A. Ardans. Hybrid Pathogen DNA Detector:Users? Manual v1.5. Office of Scientific and Technical Information (OSTI), January 2004. http://dx.doi.org/10.2172/15014078.
Full text
2
West, Abby L., Mark H. Griep, Dan P. Cole, and Shashi P. Karna. Gold Nanocluster-DNase 1 Hybrid Materials for DNA Contamination Sensing. Fort Belvoir, VA: Defense Technical Information Center, January 2014. http://dx.doi.org/10.21236/ada610452.
Full text
3
Womack, James E., Moshe (Morris) Soller, and Jacques S. Beckmann. Mapping the Dairy Cattle Genome Using Somatic Cell Hybrids and Recombinant DNA Technology. United States Department of Agriculture, September 1986. http://dx.doi.org/10.32747/1986.7566850.bard.
Full text
4
Humphries, David, Martin Pollard, Chris Elkin, Karl Petermann, Charles Reiter, and Mario Cepeda. New high performance hybrid magnet plates for DNA separation andbio-technology applications. Office of Scientific and Technical Information (OSTI), August 2004. http://dx.doi.org/10.2172/861015.
Full text
5
Tel-Zur, Neomi, and Jeffrey J. Doyle. Role of Polyploidy in Vine Cacti Speciation and Crop Domestication. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7697110.bard.
Full textAbstract:
1. Abstract: Over the past 25 years, vine cacti of the genera Hylocereus and Selenicereus have been introduced into Israel and southern California as new exotic fruit crops. The importance of these crops lies in their high water use efficiency and horticultural potential as exotic fruit crops. Our collaboration focused on the cytological, molecular and evolutionary aspects of vine cacti polyploidization to confront the agricultural challenge of genetic improvement, ultimately to improve success of vine cacti as commercial fruit crop plants. More specifically, we worked on the: 1- Identification of the putative ancestor(s) of the tetraploid H. megalanthus; 2- Determination of the number of origins of H. megalanthus (single vs. multiple origins of polyploidy); 3- Cytogenetic analysis of BC1 and F1 hybrids; 4- Determination of important agricultural traits and the selection of superior hybrids for cultivation. The plant material used in this study comprised interspecific Hylocereus F1 and first backcross (BC1) hybrids, nine Hylocereus species (58 genotypes), nine Selenicereus species (14 genotypes), and four Epiphyllum genotypes. Two BC1 hexaploids (BC-023 and BC-031) were obtained, a high ploidy level that can be explained only by a fertilization event between one unreduced female gamete from the triploid hybrid and a balanced gamete from the pollen donor, the diploid H. monacanthus. These findings are scientific evidence that support the possibility that “hybridization followed by chromosome doubling” could also occur in nature. Cytomixis, the migration of chromatin between adjacent cells through connecting cytoplasmatic channels, was observed in vine cacti hybrids and may thus imply selective DNA elimination in response to the allopolyploidization process. Evidence from plastid and nrDNA internal transcribed spacers (ITS) sequences support the placement of H. megalanthus within a monophyletic Hylocereus group. Furthermore, both plastid and ITS datasets are most consistent with a conclusion that this tetraploid species is an autopolyploid, despite observations that the species appears to be morphologically intermediate between Hylocereus and Selenicereus. Although the possibility of very narrow allopolyploidly (i.e., derivation from parents that are barely diverged from each other such as closely related species in the same genus) cannot be ruled out entirely based on our data (in part due to the unavailability of Hylocereus species considered to be morphologically the closest relatives of H. megalanthus), the possibility of H. megalanthus representing an intergeneric cross (i.e., Hylocereus × Selenicereus) seems extremely unlikely. Interestingly, the process of homogenization of ITS sequences (concerted evolution) is either incomplete or lacking in both Hylocereus and Selenicereus, and the inclusion of several artificial hybrids in the molecular study revealed the potential for biparental plastid inheritance in Hylocereus. The most important agricultural implication of this research project was the information collected for F1 and BC1 hybrids. Specifically, this project concluded with the selection of four superior hybrids in terms of fruit quality and potential yields under extreme high temperatures. These selected hybrids are self-compatible, avoiding the need for hand cross pollination to set fruits, thus reducing manpower costs. We recently offered these hybrids to growers in Israel for prioritized rapid evaluation and characterization.
6
Dugan, L. Elucidation of the Mechanism of Gene Silencing using Small Interferin RNA: DNA Hybrid Molecules. Office of Scientific and Technical Information (OSTI), February 2006. http://dx.doi.org/10.2172/900164.
Full text
7
Lin, Kuan-Jiuh, Watson Kuo, and Ching-Hsiu Tsai. Hybrid Semiconductor Nanostructures as Unique Capabilities in the Direct Detection of Proteins, Viruses, and DNA. Fort Belvoir, VA: Defense Technical Information Center, February 2008. http://dx.doi.org/10.21236/ada476323.
Full text
8
Sink, Ken, Shamay Izhar, and Abraham Nachmias. Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, February 1996. http://dx.doi.org/10.32747/1996.7613010.bard.
Full textAbstract:
Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100 Gy. The asymmetric plants had eggplant morphology and regenerated from one hybrid callus with 6.29 average size tomato chromosomes. Limited amounts of EP DNA were found in the three somatic hybrid plants H18-1 to -3 by dot-blot hybridization with probe pTHG2, to be equivalent to 6.23, 5.41, and 5.95 % EP, respectively. RFLP analysis of Lycopersicon esculentum and L. pennellii specific chromosomes revealed that only fragments of 8 to 10 out of the 24 EP chromosomes are present in the asymmetric plants. Transgenic plants 2-3, 2-4 and 10-3 were found resistant to verticillium; suggesting successful transfer of the Ve complex from S. torvum to eggplant.
9
Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, April 1995. http://dx.doi.org/10.32747/1995.7613014.bard.
Full textAbstract:
Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and berry shape. In V. vinifera, the occurrence of specific markers was correlated with seedlessness, muscat flavor and fruit color. Polymorphic RAPD bands included single copy as well as repetitive DNA. Mapping procedures were improved by optimizing PCR parameters with grape DNA; by the development of an efficient DNA extraction protocol; and with the use of long (17- to 24-mer) primers which amplify more polymorphic loci per primer. DNA fingerprint analysis with RAPD markers indicated that vinifera cultivars could be separated readily with RAPD profiles. Pinot gris, thought to be a sort of Pinot noir, differed by 12 bands from Pinot noir. This suggests that while Pinot gris may be related to Pinot noir, it is not likely to be a clone. The techniques developed in this project are now being further refined to use marker-assisted selection in breeding programs for the early selection of elite seedlings. Furthermore, the stage has been set for future attempts to clone genes from grapes based upon map locations.
10
Levin, Ilan, John W. Scott, Moshe Lapidot, and Moshe Reuveni. Fine mapping, functional analysis and pyramiding of genes controlling begomovirus resistance in tomato. United States Department of Agriculture, November 2014. http://dx.doi.org/10.32747/2014.7594406.bard.
Full textAbstract:
Abstract. Tomato yellow leaf curl virus (TYLCV), a monopartitebegomovirus, is one of the most devastating viruses of cultivated tomatoes and poses increasing threat to tomato production worldwide. Because all accessions of the cultivated tomato are susceptible to these viruses, wild tomato species have become a valuable resource of resistance genes. QTL controlling resistance to TYLCV and other begomoviruses (Ty loci) were introgressed from several wild tomato species and mapped to the tomato genome. Additionally, a non-isogenic F₁diallel study demonstrated that several of these resistance sources may interact with each other, and in some cases generate hybrid plants displaying lower symptoms and higher fruit yield compared to their parental lines, while their respective resistance genes are not necessarily allelic. This suggests that pyramiding genes originating from different resistance sources can be effective in obtaining lines and cultivars which are highly resistant to begomoviruses. Molecular tools needed to test this hypothesis have been developed by our labs and can thus significantly improve our understanding of the mechanisms of begomovirus resistance and how to efficiently exploit them to develop wider and more durable resistance. Five non-allelic Ty loci with relatively major effects have been mapped to the tomato genome using molecular DNA markers, thereby establishing tools for efficient marker assisted selection, pyramiding of multiple genes, and map based gene cloning: Ty-1, Ty-2, Ty-3, Ty-4, and ty-5. This research focused on Ty-3 and Ty-4 due to their broad range of resistance to different begomoviruses, including ToMoV, and on ty-5 due to its exceptionally high level of resistance to TYLCV and other begomoviruses. Our aims were: (1) clone Ty-3, and fine map Ty-4 and Ty-5 genes, (2)introgress each gene into two backgroundsand develop semi isogenic lines harboring all possible combinations of the three genes while minimizing linkage-drag, (3) test the resulting lines, and F₁ hybrids made with them, for symptom severity and yield components, and (4) identify and functionally characterize candidate genes that map to chromosomal segments which harbor the resistance loci. During the course of this research we have: (1) found that the allelic Ty-1 and Ty-3 represent two alternative alleles of the gene coding DFDGD-RDRP; (2) found that ty-5is highly likely encoded by the messenger RNA surveillance factor PELOTA (validation is at progress with positive results); (3) continued the map-based cloning of Ty-4; (4) generated all possible gene combinations among Ty-1, Ty-3 and ty-5, including their F₁ counterparts, and tested them for TYLCV and ToMoV resistance; (5) found that the symptomless line TY172, carrying ty-5, also carries a novel allele of Ty-1 (termed Ty-1ⱽ). The main scientific and agricultural implications of this research are as follows: (1) We have developed recombination free DNA markers that will substantially facilitate the introgression of Ty-1, Ty-3 and ty-5 as well as their combinations; (2) We have identified the genes controlling TYLCV resistance at the Ty-1/Ty-3 and ty-5 loci, thus enabling an in-depth analyses of the mechanisms that facilitate begomovirus resistance; (3) Pyramiding of Ty resistance loci is highly effective in providing significantly higher TYLCV resistance.