Relevant bibliographies by topics / DNA Aptamer-based biosensing

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Academic literature on the topic 'DNA Aptamer-based biosensing'

Author: Grafiati
Published: 6 September 2023

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Journal articles on the topic "DNA Aptamer-based biosensing"

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Hou, Ting, Wei Li, Lianfang Zhang, and Feng Li. "A versatile and highly sensitive homogeneous electrochemical strategy based on the split aptamer binding-induced DNA three-way junction and exonuclease III-assisted target recycling." Analyst 140, no. 16 (2015): 5748–53. http://dx.doi.org/10.1039/c5an01176k.

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A versatile and highly sensitive homogeneous electrochemical biosensing platform has been developed for an ATP assay based on split aptamer binding-induced DNA three-way junction formation and Exo III-assisted target recycling.
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Zhou, Dejian. "Quantum dot–nucleic acid/aptamer bioconjugate-based fluorimetric biosensors." Biochemical Society Transactions 40, no. 4 (July 20, 2012): 635–39. http://dx.doi.org/10.1042/bst20120059.

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Over the last 10 years, fluorescent semiconductor QD (quantum dot)–biomolecule conjugates have emerged as a powerful new sensing platform showing great potential in a wide range of applications in biosensing, environmental monitoring and disease diagnosis. The present mini-review is a brief account of the recent developments in QD–NA (nucleic acid), particularly NA aptamer, conjugate-based biosensors using the FRET (Förster resonance energy transfer) readout mechanism. It starts with a brief introduction to the NA aptamer and QD-FRET, followed by example approaches to compact QD–DNA conjugates, target readout strategies and sensing performance, and concludes with challenges and outlook for the QD–NA/aptamer bioconjugate sensors.
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Lam, Sin Yu, Hill Lam Lau, and Chun Kit Kwok. "Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application." Biosensors 12, no. 12 (December 7, 2022): 1142. http://dx.doi.org/10.3390/bios12121142.

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Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment. Therefore, it is crucial to develop low-cost, user-friendly, and portable biosensors capable of rapidly detecting these contaminants. Antibodies have traditionally been used as biorecognition elements. However, aptamers have recently been applied as biorecognition elements in aptamer-based biosensors, also known as aptasensors. The systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro technique used to generate aptamers that bind their targets with high affinity and specificity. Over the past decade, a modified SELEX method known as Capture-SELEX has been widely used to generate DNA or RNA aptamers that bind small molecules. In this review, we summarize the recent strategies used for Capture-SELEX, describe the methods commonly used for detecting and characterizing small-molecule–aptamer interactions, and discuss the development of aptamer-based biosensors for various applications. We also discuss the challenges of the Capture-SELEX platform and biosensor development and the possibilities for their future application.
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Trunzo, Nevina E., and Ka Lok Hong. "Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications." International Journal of Molecular Sciences 21, no. 14 (July 18, 2020): 5074. http://dx.doi.org/10.3390/ijms21145074.

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Aptamers have gained an increasing role as the molecular recognition element (MRE) in diagnostic assay development, since their first conception thirty years ago. The process to screen for nucleic acid-based binding elements (aptamers) was first described in 1990 by the Gold Laboratory. In the last three decades, many aptamers have been identified for a wide array of targets. In particular, the number of reports on investigating single-stranded DNA (ssDNA) aptamer applications in biosensing and diagnostic platforms have increased significantly in recent years. This review article summarizes the recent (2015 to 2020) progress of ssDNA aptamer research on bacteria, proteins, and lipids of bacterial origins that have implications for human infections. The basic process of aptamer selection, the principles of aptamer-based biosensors, and future perspectives will also be discussed.
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Hu, Yingxin, Zhiyu Wang, Zhekun Chen, and Linqiang Pan. "Switching the activity of Taq polymerase using clamp-like triplex aptamer structure." Nucleic Acids Research 48, no. 15 (July 9, 2020): 8591–600. http://dx.doi.org/10.1093/nar/gkaa581.

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Abstract In nature, allostery is the principal approach for regulating cellular processes and pathways. Inspired by nature, structure-switching aptamer-based nanodevices are widely used in artificial biotechnologies. However, the canonical aptamer structures in the nanodevices usually adopt a duplex form, which limits the flexibility and controllability. Here, a new regulating strategy based on a clamp-like triplex aptamer structure (CLTAS) was proposed for switching DNA polymerase activity via conformational changes. It was demonstrated that the polymerase activity could be regulated by either adjusting structure parameters or dynamic reactions including strand displacement or enzymatic digestion. Compared with the duplex aptamer structure, the CLTAS possesses programmability, excellent affinity and high discrimination efficiency. The CLTAS was successfully applied to distinguish single-base mismatches. The strategy expands the application scope of triplex structures and shows potential in biosensing and programmable nanomachines.
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Li, Sen, Defu He, Shuning Li, Ruipeng Chen, Yuan Peng, Shuang Li, Dianpeng Han, et al. "Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination." Biosensors 12, no. 6 (June 2, 2022): 387. http://dx.doi.org/10.3390/bios12060387.

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Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace BPA. Moreover, the capture unit was prepared by immobilizing thiolated (SH)-BPA aptamer complementary DNA on AuNP-coated magnetic halloysite nanotubes (MNTs@AuNPs), and SH-BPA aptamer-modified Au@4-MBA@Ag core–shell SERS nanotags acted as signal units. By the complementary pairing of the BPA aptamer and the corresponding DNA, MNTs@AuNPs and Au@4-MBA@AgCS were linked together through hybridization-ligation, which acted as the SERS substrate. In the absence of BPA, the constructed aptamer sensor generated electromagnetic enhancement and plasmon coupling to improve the sensitivity of SERS substrates. Owing to the high affinity between BPA and the aptamer, the aptamer probe bound to BPA was separated from the capture unit by an externally-induced magnetic field. Thus, the Raman intensity of the MNTs@AuNP-Ag@AuCS core–satellite assemblies was negatively correlated with the BPA concentration. High sensitivity measurements of BPA might be performed by determining the decline in SERS signal strength together with concentration variations. The proposed aptasensor is a promising biosensing platform for BPA detection.
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Zhang, Song Bai, Pei Zhen Han, Ping Lu, Xia Hu, Li Ying Zheng, Xue Wen Liu, Guang Yu Shen, Ji Lin Lu, Li Ping Qiu, and Shi Biao Zhou. "Reusable Electrochemical Aptasensor for Sensitive Detection of Small Molecules Based on Structure-Switching Hairpin Probe." Advanced Materials Research 791-793 (September 2013): 988–91. http://dx.doi.org/10.4028/www.scientific.net/amr.791-793.988.

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A reusable electrochemical biosensing strategy based on structure-switching hairpin probe for the detection of small molecules is proposed using cocaine as the model analyte. Aptamer probe hybridized with the immobilized signal probe to form DNA duplex. When target small molecule was added, competition between target molecule and the signal probe with the aptamer probe happened, which induced the signal probe from stretched duplex to hairpin structure. By measuring ac current voltammetry, the target molecule can be sensitively detected in a linear dynamic range from 1 nM-1000 nM with a low detection limit of 0.7 nM. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.
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Hsieh, Pi-Chou, Hui-Ting Lin, Wen-Yih Chen, Jeffrey J. P. Tsai, and Wen-Pin Hu. "The Combination of Computational and Biosensing Technologies for Selecting Aptamer against Prostate Specific Antigen." BioMed Research International 2017 (2017): 1–11. http://dx.doi.org/10.1155/2017/5041683.

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Herein, we report a method of combining bioinformatics and biosensing technologies to select aptamers against prostate specific antigen (PSA). The main objective of this study is to select DNA aptamers with higher binding affinity for PSA by using the proposed method. Based on the five known sequences of PSA-binding aptamers, we adopted the functions of reproduction and crossover in the genetic algorithm to produce next-generation sequences for the computational and experimental analysis. RNAfold web server was utilized to analyze the secondary structures, and the 3-dimensional molecular models of aptamer sequences were generated by using RNAComposer web server. ZRANK scoring function was used to rerank the docking predictions from ZDOCK. The biosensors, the quartz crystal microbalance (QCM) and a surface plasmon resonance (SPR) instrument, were used to verify the binding ability of selected aptamer for PSA. By carrying out the simulations and experiments after two generations, we obtain one aptamer that can have the highest binding affinity with PSA, which generates almost 2-fold and 3-fold greater measured signals than the responses produced by the best known DNA sequence in the QCM and SPR experiments, respectively.
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Xu, Ruiting, Leixin Ouyang, Heyi Chen, Ge Zhang, and Jiang Zhe. "Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles." Biosensors 13, no. 4 (April 13, 2023): 474. http://dx.doi.org/10.3390/bios13040474.

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The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has gained extensive attention for biomolecular detection due to its high selectivity, affinity, reproducibility, and robustness. Concurrently, biosensing with nanoparticles has been widely used for its high carrier capacity, stability and feasibility of incorporating optical and catalytic activity, and enhanced diffusivity. Biosensors based on aptamers and nanoparticles utilize the combination of their advantages and have become a promising technology for detecting of a wide variety of biomolecules with high sensitivity, reliability, specificity, and detection speed. Via various sensing mechanisms, target biomolecules have been quantified in terms of optical (e.g., colorimetric and fluorometric), magnetic, and electrical signals. In this review, we summarize the recent advances in and compare different aptamer–nanoparticle-based biosensors by nanoparticle types and detection mechanisms. We also share our views on the highlights and challenges of the different nanoparticle-aptamer-based biosensors.
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Grechkin, Yaroslav A., Svetlana L. Grechkina, Emil A. Zaripov, Svetlana V. Fedorenko, Asiya R. Mustafina, and Maxim V. Berezovski. "Aptamer-Conjugated Tb(III)-Doped Silica Nanoparticles for Luminescent Detection of Leukemia Cells." Biomedicines 8, no. 1 (January 13, 2020): 14. http://dx.doi.org/10.3390/biomedicines8010014.

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DNA aptamers have many benefits for cell imaging, such as high affinity and specificity, easiness of chemical functionalization, and low cost of production. Among known aptamers, Sgc8-aptamer was selected against acute lymphoblastic leukemia cells with a dissociation constant in a nanomolar range. The aptamer was previously used for the covalent coupling with fluorescent and magnetic nanoparticles, as well as for the fabrication of aptamer-based biosensors. Among commonly used fluorescent tags, lanthanide nanoparticles offer stable luminescence with narrow, well-resolved emission peaks and the absence of photoblinking. In other words, lanthanide nanoparticles could serve as luminescence reporters and be used in biosensing. In our study, we conjugated amino- and carboxyl-modified silica-coated terbium (III) thiacalix[4]arenesulfonate luminescent nanoparticles with Sgc8-aptamer and showed the ability of the aptamer-conjugated nanoparticles to detect leukemia cells using fluorescence microscopy. In addition, we conducted a cell viability assay and confirmed that the nanoparticles do not induce spontaneous cell apoptosis or necrosis and could be potentially used for bioimaging applications.
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Book chapters on the topic "DNA Aptamer-based biosensing"

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Söylemez, Fatma, and Çağatay Han Türkseven. "Aptamers and Possible Effects on Neurodegeneration." In Neuroprotection - New Approaches and Prospects. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.89621.

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Aptamers are a new class of recognizing agents which are defined as short biomolecules like oligonucleotides and peptides that are used in diagnostics and therapeutics. They can bind to specific targets with extremely high affinity based on their structural conformations. It is believed that in the near future, aptamers could replace monoclonal antibody. The biggest advantage of using aptamers is that the process is in vitro in nature and does not require the use of animals and they also have unique properties, such as thermal stability, low cost, and unlimited applications. Aptamers have been studied as a biomaterial in numerous investigations concerning their use as a diagnostic and therapeutic tool and biosensing probe. DNA aptamers were also used for the diagnosis and treatment of neurodegeneration and neurodegenerative diseases. For example, functional nucleic acid aptamers have been developed to detect Aβ fragments in Alzheimer’s brain hippocampus tissue samples. Aptamers are promising materials for diverse areas, not just as alternatives to antibodies but as the core components of medical equipment. Although they are in the preliminary stages of development, results are quite encouraging, and it seems that aptamer research has a very bright future in neuroscience.
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Conference papers on the topic "DNA Aptamer-based biosensing"

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Katsura, Taiji, Kenzo Maehashi, Kazuhiko Matsumoto, Kagan Kerman, Yuzuru Takamura, and Eiichi Tamiya. "DNA Aptamer-Based Biosensing of Immunoglobulin E Using Carbon Nanotube Field-Effect Transistors." In 2006 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2006. http://dx.doi.org/10.7567/ssdm.2006.a-6-4.

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