Journal articles on the topic 'DMSL method'
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1
Walczak, Mariusz, Aleksander Świetlicki, Mirosław Szala, Marcin Turek, and Dariusz Chocyk. "Shot Peening Effect on Sliding Wear in 0.9% NaCl of Additively Manufactured 17-4PH Steel." Materials 17, no. 6 (March 18, 2024): 1383. http://dx.doi.org/10.3390/ma17061383.
Abstract:
The growing demand for modern steels showing corrosion and tribological resistance has led to their increased use in the production of medical devices. This study analyzed the effect of shot peening on wear resistance in 0.9% NaCl solution of 17-4PH steel produced by direct laser metal sintering (DMLS) technology. The study’s novelty relies on revealing the effect of shot peening (SP) surface treatment on the wet sliding wear resistance of 17-4PH steel produced with DMLS. Moreover, in the context of 17-4PH steel application for medical devices, the 0.9% NaCl tribological environment were selected, and SP processes were conducted using steel CrNi shot and ceramic (ZrO2) beads. The up-to-date scientific literature has not identified these gaps in the research. DMLS technology makes it possible to obtain products with complex architectures, but it also faces various challenges, including imperfections in the surface layer of products due to the use of 3D printing technology itself. The chemical and phase composition of the materials obtained, Vickers hardness, surface roughness, and microscopic and SEM imaging were investigated. Tribological tests were carried out using the ball-on-disc method, and the surfaces that showed traces of abrasion to identify wear mechanisms were subjected to SEM analysis. The XRD phase analysis indicates that austenite and martensite were found in the post-production state, while a higher martensitic phase content was found in peened samples due to phase transformations. The surface hardness of the peened samples increased by more than double, and the post-treatment roughness increased by 12.8% after peening CrNi steels and decreased by 7.8% after peening ZrO2 relative to the reference surfaces. Roughness has an identifiable effect on sliding wear resistance. Higher roughness promotes material loss. After the SP process, the coefficient of friction increased by 15.5% and 20.7%, while the wear factor (K) decreased by 25.9% and 32.7% for the samples peened with CrNi steels and ZrO2, respectively. Abrasive and adhesive mechanisms were dominant, featured with slight fatigue. The investigation showed a positive effect of SP on the tribological properties of DMSL 17-4PH.
2
Kinsey, Joanna D., and David J. Kieber. "Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples." Limnology and Oceanography: Methods 14, no. 3 (January 12, 2016): 196–209. http://dx.doi.org/10.1002/lom3.10081.
3
Zhang, Xizhen, Aiwu Zhang, Yuan Sun, Juan Wang, Haiyang Pang, Jinbang Peng, Yunsheng Chen, et al. "Deep Multi-Order Spatial–Spectral Residual Feature Extractor for Weak Information Mining in Remote Sensing Imagery." Remote Sensing 16, no. 11 (May 29, 2024): 1957. http://dx.doi.org/10.3390/rs16111957.
Abstract:
Remote sensing images (RSIs) are widely used in various fields due to their versatility, accuracy, and capacity for earth observation. Direct application of RSIs to harvest optimal results is generally difficult, especially for weak information features in the images. Thus, extracting the weak information in RSIs is reasonable to promote further applications. However, the current techniques for weak information extraction mainly focus on spectral features in hyperspectral images (HSIs), and a universal weak information extraction technology for RSI is lacking. Therefore, this study focused on mining the weak information from RSIs and proposed the deep multi-order spatial–spectral residual feature extractor (DMSRE). The DMSRE considers the global information and three-dimensional cube structures by combining low-rank representation, high-order residual quantization, and multi-granularity spectral segmentation theories. This extractor obtains spatial–spectral features from two derived sequences (deep spatial–spectral residual feature (DMSR) and deep spatial–spectral coding feature (DMSC)), and three RSI datasets (i.e., Chikusei, ZY1-02D, and Pasture datasets) were employed to validate the DMSRE method. Comparative results of the weak information extraction-based classifications (including DMSR and DMSC) and the raw image-based classifications showed the following: (i) the DMSRs can improve the classification accuracy of individual classes in fine classification applications (e.g., Asphalt class in the Chikusei dataset, from 89.12% to 95.99%); (ii) the DMSC improved the overall accuracy in rough classification applications (from 92.07% to 92.78%); and (iii) the DMSC improved the overall accuracy in RGB classification applications (from 63.25% to 63.6%), whereas DMSR improved the classification accuracy of individual classes on the RGB image (e.g., Plantain classes in the Pasture dataset, from 32.49% to 39.86%). This study demonstrates the practicality and capability of the DMSRE method to promote target recognition on RSIs and presents an alternative technique for weak information mining on RSIs, indicating the potential to extend weak information-based applications of RSIs.
4
Kong, Yingying, and Si Liu. "DMSC-GAN: A c-GAN-Based Framework for Super-Resolution Reconstruction of SAR Images." Remote Sensing 16, no. 1 (December 21, 2023): 50. http://dx.doi.org/10.3390/rs16010050.
Abstract:
Synthetic Aperture Radar (SAR) imagery is significant in remote sensing, but the limited spatial resolution results in restricted detail and clarity. Current super-resolution methods confront challenges such as complex network structure, insufficient sensing capability, and difficulty extracting features with local and global dependencies. To address these challenges, DMSC-GAN, a SAR image super-resolution technique based on the c-GAN framework, is introduced in this study. The design objective of DMSC-GAN is to enhance the flexibility and controllability of the model by utilizing conditional inputs to modulate the generated image features. The method uses an encoder–decoder structure to construct a generator and introduces a feature extraction module that combines convolutional operations with Deformable Multi-Head Self-Attention (DMSA). This module can efficiently capture the features of objects of various shapes and extract important background information needed to recover complex image textures. In addition, a multi-scale feature extraction pyramid layer helps to capture image details at different scales. DMSC-GAN combines perceptual loss and feature matching loss and, with the enhanced dual-scale discriminator, successfully extracts features from SAR images for high-quality super-resolution reconstruction. Extensive experiments confirm the excellent performance of DMSC-GAN, which significantly improves the spatial resolution and visual quality of SAR images. This framework demonstrates strong capabilities and potential in advancing super-resolution techniques for SAR images.
5
Dong, Lihu, Yue Zhang, Zhuo Zhang, Longfei Xie, and Fengri Li. "Comparison of Tree Biomass Modeling Approaches for Larch (Larix olgensis Henry) Trees in Northeast China." Forests 11, no. 2 (February 11, 2020): 202. http://dx.doi.org/10.3390/f11020202.
Abstract:
Accurate quantification of tree biomass is critical and essential for calculating carbon storage, as well as for studying climate change, forest health, forest productivity, nutrient cycling, etc. Tree biomass is typically estimated using statistical models. Although various biomass models have been developed thus far, most of them lack a detailed investigation of the additivity properties of biomass components and inherent correlations among the components and aboveground biomass. This study compared the nonadditive and additive biomass models for larch (Larix olgensis Henry) trees in Northeast China. For the nonadditive models, the base model (BM) and mixed effects model (MEM) separately fit the aboveground and component biomass, and they ignore the inherent correlation between the aboveground and component biomass of the same tree sample. For the additive models, two aggregated model systems with one (AMS1) and no constraints (AMS2) and two disaggregated model systems without (DMS1) and with an aboveground biomass model (DMS2) were fitted simultaneously by weighted nonlinear seemingly unrelated regression (NSUR) and applied to ensure additivity properties. Following this, the six biomass modeling approaches were compared to improve the prediction accuracy of these models. The results showed that the MEM with random effects had better model fitting and performance than the BM, AMS1, AMS2, DMS1, and DMS2; however, when no subsample was available to calculate random effects, AMS1, AMS2, DMS1, and DMS2 could be recommended. There was no single biomass modeling approach to predict biomass that was best for all aboveground and component biomass except for MEM. The overall ranking of models based on the fit and validation statistics obeyed the following order: MEM > DMS1 > AMS2 > AMS1> DMS2 > BM. This article emphasized more on the methodologies and it was expected that the methods could be applied by other researchers to develop similar systems of the biomass models for other species, and to verify the differences between the aggregated and disaggregated model systems. Overall, all biomass models in this study have the benefit of being able to predict aboveground and component biomass for larch trees and to be used to predict biomass of larch plantations in Northeast China.
6
Mishra, Pawan Kumar, and Adam Ekielski. "A Simple Method to Synthesize Lignin Nanoparticles." Colloids and Interfaces 3, no. 2 (June 10, 2019): 52. http://dx.doi.org/10.3390/colloids3020052.
Abstract:
The self-assembly of lignin (molecular and supramolecular) is driven mainly by non-covalent interactions, and the nature of the solvents and antisolvents directly affect the driving forces. The lignin particle is usually formed by noncovalently bonded cylindrical subunits. In this paper, we report a simple method which can be used to synthesize lignin nanoparticles by using spray freezing. The method is based on two properties of dimethyl sulfoxide (DMSO) that are excellent lignin solubility and a high melting point. Based on these two properties, kraft lignin solution in DMSO was sprayed onto liquid nitrogen-cooled copper plates using a handheld spray. The high melting point of DMSO caused immediate freezing and particle formation. The obtained particles were characterized for their size and morphology using dynamic light scattering (DLS), as well as scanning electron microscopy (SEM). Nano-range polydispersed particles were obtained by spraying 0.05% of lignin onto DMSO. This method can avoid lignin–solvent–antisolvent interactions, and can also be used to study lignin–lignin (subunits) and lignin–DMSO interactions.
7
Griebler, Christian, and Doris Slezak. "Microbial Activity in Aquatic Environments Measured by Dimethyl Sulfoxide Reduction and Intercomparison with Commonly Used Methods." Applied and Environmental Microbiology 67, no. 1 (January 1, 2001): 100–109. http://dx.doi.org/10.1128/aem.67.1.100-109.2001.
Abstract:
ABSTRACT A new method to determine microbial (bacterial and fungal) activity in various freshwater habitats is described. Based on microbial reduction of dimethyl sulfoxide (DMSO) to dimethyl sulfide (DMS), our DMSO reduction method allows measurement of the respiratory activity in interstitial water, as well as in the water column. DMSO is added to water samples at a concentration (0.75% [vol/vol] or 106 mM) high enough to compete with other naturally occurring electron acceptors, as determined with oxygen and nitrate, without stimulating or inhibiting microbial activity. Addition of NaN3, KCN, and formaldehyde, as well as autoclaving, inhibited the production of DMS, which proves that the reduction of DMSO is a biotic process. DMSO reduction is readily detectable via the formation of DMS even at low microbial activities. All water samples showed significant DMSO reduction over several hours. Microbially reduced DMSO is recovered in the form of DMS from water samples by a purge and trap system and is quantified by gas chromatography and detection with a flame photometric detector. The DMSO reduction method was compared with other methods commonly used for assessment of microbial activity. DMSO reduction activity correlated well with bacterial production in predator-free batch cultures. Cell-production-specific DMSO reduction rates did not differ significantly in batch cultures with different nutrient regimes but were different in different growth phases. Overall, a cell-production-specific DMSO reduction rate of 1.26 × 10−17 ± 0.12 × 10−17 mol of DMS per produced cell (mean ± standard error;R 2 = 0.78) was calculated. We suggest that the relationship of DMSO reduction rates to thymidine and leucine incorporation is linear (the R 2 values ranged from 0.783 to 0.944), whereas there is an exponential relationship between DMSO reduction rates and glucose uptake, as well as incorporation (the R 2 values ranged from 0.821 to 0.931). Based on our results, we conclude that the DMSO reduction method is a nonradioactive alternative to other methods commonly used to assess microbial activity.
8
Ren, Shengbing, Wangbo Shen, Chaudry Siddique, and You Li. "Self-Adaptive Deep Multiple Kernel Learning Based on Rademacher Complexity." Symmetry 11, no. 3 (March 5, 2019): 325. http://dx.doi.org/10.3390/sym11030325.
Abstract:
The deep multiple kernel learning (DMKL) method has caused widespread concern due to its better results compared with shallow multiple kernel learning. However, existing DMKL methods, which have a fixed number of layers and fixed type of kernels, have poor ability to adapt to different data sets and are difficult to find suitable model parameters to improve the test accuracy. In this paper, we propose a self-adaptive deep multiple kernel learning (SA-DMKL) method. Our SA-DMKL method can adapt the model through optimizing the model parameters of each kernel function with a grid search method and change the numbers and types of kernel function in each layer according to the generalization bound that is evaluated with Rademacher chaos complexity. Experiments on the three datasets of University of California—Irvine (UCI) and image dataset Caltech 256 validate the effectiveness of the proposed method on three aspects.
9
Tang, Jingjing, and Xilong Qiu. "Preparation of Dendritic Mesoporous Silica Nanomaterials Loaded with Toosendanin-Fumaracetin and its Anti-Peripheral Pain Study." Highlights in Science, Engineering and Technology 21 (December 4, 2022): 281–85. http://dx.doi.org/10.54097/hset.v21i.3172.
Abstract:
Objective: A novel dendritic mesoporous silica nanomaterials (DMSN) was prepared and the traditional Chinese Medicine Component "Toosendanin-Tetrahydropalmatine" was loaded into DMSN in order to improve the solubility of Tetrahydropalmatine B, reduce the toxicity of Toosendanin, and enhance the anti-peripheral pain effect of the traditional. Chinese Medicine "Toosendanin-Tetrahydropalmatine" through preliminary pharmacodynamics study. Methods: DMSN were prepared using the sol-gel method and characterized by scanning electron microscope (SEM) and Fourier Transform Infrared Spectrometer (FT-IR). The preliminary study of the anti-peripheral pain pharmacodynamics of the drug-loaded system in mice was evaluated by the hot plate method. Results: The prepared DMSN has an excellent spherical shape in SEM, the size distribution of the DMSN is about 200nm, the size distribution is uniform, and the dispersion is good. The infrared results showed that the synthesized DMSN had organic functional groups and chemical structures. Compared with the control group, the pain threshold of DMSN loaded with the Toosendanin-tetrahydropalmatine group was prolonged by 45 seconds. Conclusion: The synthesized DMSN loaded with Toosendanin and Tetrahydropalmatine could release stably and continuously and had the effect of extending the pain threshold of mice. Therefore, DMSN drug delivery system has potential value in improving the bioabsorption of dangerous drugs and enhancing the bioavailability of poorly soluble drugs.
10
Page, Cheryl, and Jessica Rebeiro. "Évaluation de l’efficacité d’un programme de formation sur l’administration par bolus intraveineux de cellules souches hématopoïétiques cryoconservées." Canadian Oncology Nursing Journal 33, no. 2 (April 28, 2023): 207–14. http://dx.doi.org/10.5737/23688076332207.
Abstract:
Les infirmières autorisées (inf. aut.) d’un centre de traitement cellulaire ont bénéficié d’un programme de formation sur l’administration par bolus intraveineux de cellules cryoconservées. Les deux principales méthodes servant à administrer les produits de cellules souches hématopoïétiques (CSH) cryoconservées à l’aide de diméthylsulfoxyde (DMSO) sont la perfusion par gravité et le bolus intraveineux. Quelle que soit la voie choisie, le DMSO peut provoquer des réactions d’hypersensibilité. La perfusion par gravité étant plus lente, le DMSO y entraîne moins de réactions. Toutefois, l’exposition prolongée au DMSO une fois les cellules décongelées accroît le risque de dommages cellulaires. La méthode du bolus intraveineux, plus rapide, réduit le risque de dommages cellulaires, de même que le temps de travail du personnel. Un examen des facteurs ambiants réalisé dans des centres de greffe du Canada a montré que, dans la plupart des établissements traitant des adultes, les infirmières s’occupent des perfusions par gravité, mais que lorsque les cellules souches hématopoïétiques cryoconservées doivent être injectées par bolus intraveineux, la procédure est accomplie par un médecin. Dans le centre dont il est question ici, le bolus intraveineux était administré soit par un médecin, soit par une infirmière praticienne (IP). Comme le nombre de greffes augmente, il fallait plus de personnes capables de réaliser cette procédure. Pour conserver les bénéfices actuels de la méthode du bolus intraveineux et augmenter la capacité du programme de greffes hématopoïétiques, on a transféré la tâche d’administrer les cellules souches par bolus intraveineux, qui revenait auparavant aux IP et aux médecins, aux infirmières autorisées. Un bon programme de formation a donc été élaboré, puis évalué; il utilise l’apprentissage par simulation pour aider les infirmières en oncologie à maîtriser cette nouvelle technique.
11
Jiao, Junke, Shengyuan Sun, Zifa Xu, Jiale Wang, Liyuan Sheng, and Jicheng Gao. "Fabricating Inner Channels in Laser Additive Manufacturing Process via Thin-Plate-Preplacing Method." Materials 16, no. 19 (September 26, 2023): 6406. http://dx.doi.org/10.3390/ma16196406.
Abstract:
This paper presents a hybrid manufacturing process for the preparation of complex cavity structure parts with high surface quality. Firstly, laser precision packaging technology is utilized to accurately connect a thin plate to a substrate with microchannel. Secondly, Direct Metal Laser-Sintering (DMLS) technology is utilized to completely shape the part. The morphology and microstructure of laser encapsulated specimens and DMLS molded parts were investigated. The results show that the thin plate and the substrate can form a good metallurgical bond. The lowest surface roughness of the DMLS molded parts was 1.18 μm. The perpendicularity between the top of the microchannel and the side wall was optimal when the laser power was 240 W. Consequently, the hybrid manufacturing process effectively solves the problems of poor surface quality and powder sticking of closed inner cavities. The method effectively eliminates the defects of adhesive powder in the inner cavity of the DMLS microchannel, improves the finish, and solves the problem that mechanical tools cannot be processed inside the microchannel, which lays the foundation for the research of DMLS high-quality microchannel process.
12
Dangtungee, Rapeephun, Suchart Siengchin, and Chaisiri Kitpaosong. "Novel Method for Recycle Epoxy Resin from Waste Printed Circuit Board." Advanced Materials Research 931-932 (May 2014): 90–94. http://dx.doi.org/10.4028/www.scientific.net/amr.931-932.90.
Abstract:
This research was aimed to study and develop a method for recycle epoxy resin from waste printed circuit board by hot solvent methods and DMSO/NaOH catalytic extraction method. It was found that the pressure tank system, sub set of hot solvent method, variable as following; pressure of 15 bar, temperature of 80 °C, reaction time for 6 h and ethyl acetate solvent presented maximum yield 50.46 %, while DMSO/NaOH catalytic system at 145 °C for 5 h was found maximum yield of 42.97 %. Recycled epoxy resin, the final product from hot solvent and DMSO/NaOH catalytic were showed similarly clear orange-yellow high viscosity. Comparatively, DMSO/NaOH system showed slightly darker color than that of hot solvent system. Functional group at 3000-2850, 1480-1540 and 1020-1040 cm-1, characterized from Fourier transform infrared spectroscopy (FT-IR), indicated that the both product were alkane, aromatic, and phenyl-O-C group, respectively. It could be confirmed that the beneficial functional group of recycled epoxy resin was appeared as a new epoxy resin.
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Sharma, A., and G. N. Purohit. "Vitrification of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization." Veterinární Medicína 53, No. 8 (September 5, 2008): 427–33. http://dx.doi.org/10.17221/1928-vetmed.
Abstract:
The <i>in vitro</i> maturation (IVM), fertilization (IVF) and morphological changes in buffalo cumulus oocyte complexes (COCs) cryopreserved by ultra rapid freezing using conventional (CON) and open-pulled straw (OPS) methods were tested. COCs were cryopreserved using a vitrification solution comprising of DPBS + 0.5M sucrose + 0.4% BSA and two concentrations (4.5 or 5.5M) of each cryoprotectant ethylene glycol (EG) and dimethylsulfoxide (DMSO) and cryopreserved by either CON or OPS method. Vitrified COCs were stored in LN<sub>2</sub> for seven days and then thawed, and morphologically normal COCs were used for IVM (<i>n</i> = 864) and IVF (<i>n</i> = 933) in two separate experiments to record (1) morphological damage of COCs due to vitrification, (2) nuclear maturation 24 h after culture (nine replicates) and (3) fertilization 24 h after insemination (10 replicates). The COCs were matured <i>in vitro</i> in TCM-199 medium using hormone supplements and fertilized using TALP-BSA. Freshly collected COCs were separately used for IVM (<i>n</i> = 110) and IVF (<i>n</i> = 130) and kept as control. The arcsin transformed data of the proportion of COCs matured or fertilized was compared by DNMR test. The highest proportion of morphologically normal COCs were seen in 5.5M EG with CON method (94.5%) and the lowest were seen in 4.5M DMSO with OPS method (82.4%). At the end of Experiment 1, it was revealed that IVM in all vitrification groups was significantly lower (<i>P</i> < 0.05) compared to control (66.4%). Amongst the various vitrification treatments the highest IVM was seen in 5.5M EG with OPS method (39.2%) and the lowest in 4.5M DMSO with CON method (19.3%). Comparison of both concentrations of EG and DMSO showed that the proportion of COCs attaining metaphase-II (M-II) increased with increasing concentration of both the cryoprotectants. However, at equal concentration of EG and DMSO the proportion of COCs attaining M-II were significantly higher in OPS method compared to CON method. In Experiment 2, a significantly higher (<i>P</i> < 0.05) IVF was seen for fresh COCs (45.4%) compared to vitrified COCs. Amongst the vitrification treatments the highest fertilization was seen for 5.5M EG with the OPS method (33.6 %) and the lowest for the 4.5M DMSO with CON method (15.17%). A dose dependant increase in the proportion of oocytes fertilized was seen with increasing concentration of both EG and DMSO [CON: 4.5M (15.2%), 5.5M (25.6%), OPS: 4.5M (21.3%) and 5.5M (27.5%)] in both CON and OPS methods. Comparison of the two cryoprotectants revealed that EG was better compared to DMSO. At equal concentrations of EG or DMSO a significantly higher (<i>P</i> < 0.05) proportion of fertilized oocytes were seen in OPS method compared to the CON method. It was concluded that developmental capacity of vitrified buffalo COCs could be improved by using OPS in comparison to conventional straws.
14
Brewer, Alison C., Philip J. Marsh, and Roger K. Patient. "A simplified method forin vivofootprinting using DMS." Nucleic Acids Research 18, no. 18 (1990): 5574. http://dx.doi.org/10.1093/nar/18.18.5574.
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Adamis, Dimitrios, Rikie M. Scholtens, Annemarieke de Jonghe, Barbara C. van Munster, Sophia E. J. A. de Rooij, and David J. Meagher. "Psychometric evaluation of the DMSS-4 in a cohort of elderly post-operative hip fracture patients with delirium." International Psychogeriatrics 28, no. 7 (February 5, 2016): 1221–28. http://dx.doi.org/10.1017/s1041610216000065.
Abstract:
ABSTRACTBackground:Delirium is a common neuropsychiatric syndrome with considerable heterogeneity in clinical profile. Rapid reliable identification of clinical subtypes can allow for more targeted research efforts.Methods:We explored the concordance in attribution of motor subtypes between the Delirium Motor Subtyping Scale 4 (DMSS-4) and the original Delirium Motor Subtyping Scale (DMSS) (assessed cross-sectionally) and subtypes defined longitudinally using the Delirium Symptom Interview (DSI).Results:We included 113 elderly patients developing DSM-IV delirium after hip-surgery [mean age 86.9 ± 6.6 years; range 65–102; 68.1% females; 25 (22.1%) had no previous history of cognitive impairment]. Concordance for the first measurement was high for both the DMSS-4 and original DMSS (k = 0.82), and overall for the DMSS-4 and DSI (k = 0.84). The DMSS-4 also demonstrated high internal consistency (McDonald's omega = 0.90). The DSI more often allocated an assessment to “no subtype” compared to the DMSS-4 and DMSS-11, which showed higher inclusion rates for motor subtypes.Conclusions:The DMSS-4 provides a rapid method of identifying motor-defined clinical subtypes of delirium and appears to be a reliable alternative to the more detailed and time-consuming original DMSS and DSI methods of subtype attribution. The DMSS-4, so far translated into three languages, can be readily applied to further studies of causation, treatment and outcome in delirium.
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Yushkin, Alexey, Alexey Balynin, Mikhail Efimov, Konstantin Pochivalov, Inna Petrova, and Alexey Volkov. "Fabrication of Polyacrylonitrile UF Membranes by VIPS Method with Acetone as Co-Solvent." Membranes 12, no. 5 (May 15, 2022): 523. http://dx.doi.org/10.3390/membranes12050523.
Abstract:
For the first time, a systematic study was carried out of the replacement of the low-volatility solvents N-methyl-2-pyrrolidone (NMP) or dimethylsulfoxide (DMSO) with the high-volatility solvent acetone in the casting solution of polyacrylonitrile (PAN). The effect of acetone’s presence in the casting solution on the performance of ultrafiltration membranes fabricated via vapor-induced phase separation (VIPS) was investigated. It was possible to replace 40% of NMP and 50% of DMSO with acetone, which resulted in the reduction of the casting solution viscosity from 70.6 down to 41.3 Pa∙s (20% PAN, NMP), and from 68.3 down to 20.6 Pa∙s (20% PAN, DMSO). It was found that 20 min of exposure to water vapor (relative humidity—85%) was sufficient to govern the phase separation, which was mainly induced by the water vapor. Regardless of the casting solution composition (15 or 20% PAN; DMSO or NMP), all membranes formed via VIPS possessed a sponge-like porous structure. The addition of acetone to the casting solution allowed the reduction of the transport pore size from 35–48 down to 8.5–25.6, depending on the casting solution composition. By varying the acetone content at constant polymer concentration, it was possible to decrease the molecular weight cut-off (MWCO) from 69 to 10 kg/mol. Membranes prepared from 20% PAN solution in an acetone/DMSO mixture had the lowest MWCO of 10 kg/mol with a water permeance of 5.1 L/(m2·h·bar).
17
Habibi, Afrooz, Ahmad Hosseini, Naser Farrokhi, Fardin Amidi, Isabel Carvalhais, António Chaveiro, and Fernando Moreira da Silva. "Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations." Canadian Journal of Animal Science 91, no. 3 (September 2011): 385–88. http://dx.doi.org/10.4141/cjas10042.
Abstract:
Habibi, A., Hosseini, A., Farrokhi, N., Amidi, F., Carvalhais, I., Chaveiro, A. and Moreira da Silva, F. 2011. Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations. Can. J. Anim. Sci. 91: 385–388. The response of vitrified mouse MII oocytes in the presence of two concentrations of cryoprotectants [vit1 (15%: 7.5% dimethyl sulfoxide (DMSO)+7.5% ethylene glycol (EG) and vit2 (30%: 15% DMSO+15% EG)] was analyzed to investigate whether reducing cryoprotectant concentrations can affect oocyte survival after cryopreservation by the cryotop method. After thawing the survival, fertilization, cleavage and blastocyst rates were compared with unfrozen oocytes. It can be concluded that 15% cryoprotectant (7.5% DMSO+7.5% EG), instead of the commonly used 30% (15% DMSO+15% EG), could be helpful by moderating the probable toxic effects of vitrification solution in mouse oocyte during vitrification by cryotop.
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Oliveira, Mayara Setúbal, Priscila Silva de Almeida-Monteiro, Larissa Teixeira Nunes, Francisco Renan Aragão Linhares, João Paulo Silva Pinheiro, Romulo Roberto Ribeiro Pinheiro, Filipe Oliveira Ferreira, Claudio Cabral Campello, and Carminda Sandra Brito Salmito-Vanderley. "Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine." Semina: Ciências Agrárias 37, no. 4 (August 30, 2016): 2167. http://dx.doi.org/10.5433/1679-0359.2016v37n4p2167.
Abstract:
Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen.
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Xia, Hua, and Sheng Hui Zhang. "Synthesis, Characterization and Mechanism of Benzamide Intercalated Kaolinite by Replacement Method." Applied Mechanics and Materials 420 (September 2013): 222–29. http://dx.doi.org/10.4028/www.scientific.net/amm.420.222.
Abstract:
Kaolinite/benzamide complex was prepared by displacement reaction of a kaolinite/dimethylsulphoxide (DMSO) intercalation complex with melted benzamide. The whole process was recorded by powder X-ray diffractometry (PXRD) and Fourier-transformed infrared spectroscopy (FTIR). Those PXRD and FT-IR indicated that there are two stages in the process of melted benzamide replacing intercalation. The first stage is the deintercalation of DMSO molecules in the kaolinite/dimethylsulphoxide intercalation complex. And the second stage is the melted benzamide intercalation.
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Song, L. N., N. Gu, and Y. Zhang. "A moderate method for preparation DMSA coated Fe3O4 nanoparticles." IOP Conference Series: Materials Science and Engineering 164 (January 2017): 012026. http://dx.doi.org/10.1088/1757-899x/164/1/012026.
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Izhnin, I. I., K. D. Mynbaev, A. V. Voitsekhovskii, and A. G. Korotaev. "Discrete mobility-spectrum analysis and its application to transport studies in HgCdTe." Journal of Applied Physics 132, no. 15 (October 21, 2022): 155702. http://dx.doi.org/10.1063/5.0097418.
Abstract:
A detailed consideration of the discrete mobility-spectrum analysis (DMSA) method and its application to transport studies in HgCdTe is given. First, a brief review of the methods of the analysis of field dependences of the Hall coefficient and conductivity in HgCdTe-based structures with a multi-carrier mobility spectrum is presented. The advantages and drawbacks of these methods, which include original mobility-spectrum analysis by Beck and Anderson, multi-carrier fitting and iterative approach by Dziuba and Górska, and the later developments of these techniques, are considered. The properties of the envelope of the mobility spectrum are analyzed, and the specifics of DMSA are presented. Analysis of some magnetic-field B dependences of conductivity tensor components σxx( B) and σxy( B) presented in the literature is performed with DMSA, and the results are compared to those obtained with other methods.
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Griebler, C. "Some applications for the DMSO-reduction method as a new tool to determine the microbial activity in water-saturated sediments." River Systems 10, no. 1-4 (September 18, 1996): 405–10. http://dx.doi.org/10.1127/lr/10/1996/405.
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Żuchowski, Grzegorz, and Krzysztof Zborowski. "The influence of solvent molecules on NMR spectrum of barbituric acid in the DMSO solution." Open Chemistry 4, no. 3 (September 1, 2006): 523–32. http://dx.doi.org/10.2478/s11532-006-0019-z.
Abstract:
AbstractThis work shows the modification of barbituric acid (BA) chemical shifts by dimethylsulphoxide (DMSO) molecules. The discussed changes are caused by creation of the H-bonded associates formed by barbituric acid with DMSO in solution. Free molecule of barbituric acid, the cluster of BA with two DMSO molecules and two different clusters of BA with four DMSO units are taken into consideration. The chemical shifts of these systems have been calculated and the obtained results have been compared with experimental data. Theoretical calculations predict a significant downfield shift for imino protons of barbituric acid involved in intermolecular-N-H...DMSO hydrogen bonds. The influence of the solvent molecules on other nuclei chemical shifts, especially protons of barbituric acid methylene group, is also reported.The calculations have involved Hartree-Fock and several Density Functional Theory methods. All methods correctly describe experimental 1H and 13C NMR spectra of barbituric acid. The best consistence between experiment and theory is observed for the BLYP functional. Four approximations of magnetic properties calculations embedded in the Gaussian’98 package have been tested. The results of the performed calculations indicate that from a practical point of view the GIAO method should be preferred.
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Sharma, A., and G. N. Purohit. "355 DEVELOPMENTAL COMPETENCE OF IMMATURE BUBALINE CUMULUS - OOCYTE COMPLEXES VITRIFIED BY THE OPEN PULLED STRAW AND CONVENTIONAL STRAW METHODS." Reproduction, Fertility and Development 19, no. 1 (2007): 293. http://dx.doi.org/10.1071/rdv19n1ab355.
Abstract:
The in vitro maturation (IVM), fertilization (IVF), and morphological changes in buffalo cumulus–oocyte complexes (COCs) cryopreserved by ultrarapid freezing using conventional (CON) and open pulled staw (OPS) methods were tested. COCs were cryopreserved using a vitrification solution comprised of Dulbecco's phosphate-buffered saline+0.5 M sucrose+0.4% BSA and two concentrations (4.5 or 5.5 M) of each cryoprotectant ethylene glycol (EG) and dimethylsulfoxide (DMSO) by either the CON or the OPS method. Vitrified COCs were stored in LN for 7 days and then thawed; morphologically normal COCs were used for IVM (n = 1070) and IVF (n = 933) in 2 separate experiments to record morphological damage of COCs due to vitrification, nuclear maturation 24 h after culture (9 replicates), and fertilization 24 h after insemination (10 replicates). The COCs were matured in vitro in TCM-199 media with hormone supplements and fertilized using TALP-BSA as described previously (Purohit et al. 2005 Anim. Reprod. Sci. 87, 229–239). Freshly collected COCs were separately used for IVM (n = 110) and IVF (n = 130) and kept as controls. The arcsin transformed data of the proportions of oocytes matured or fertilized was compared by Duncan's new multiple range test. The highest proportion of morphologically normal oocytes was seen in 5.5 M EG with the CON method (94.5%) and the lowest was seen in 4.5 M DMSO with the OPS method (82.4%). At the end of experiment 1, it was apparent that IVM in all vitrification groups was significantly lower (P < 0.05) compared to the control group (66.4%). Among the various vitrification treatments, the highest IVM occurred in 5.5 M EG with the OPS method (39.2%) and the lowest in 4.5 DMSO with the CON method (19.3%). Comparison of both concentrations of EG and DMSO showed that the proportion of COCs attaining Metaphase-II (M-II) increased with increasing concentration of both of the cryoprotectants. However, at equal concentration of EG and DMSO, the proportion of COCs attaining M-II was significantly higher in the OPS method compared to the CON method. In experiment 2, a significantly higher (P < 0.05) IVF was seen for fresh COCs (45.4%) compared to vitrified COCs. Among the vitrification treatments, the highest fertilization was seen in 5.5 M EG with the OPS method (33.6%) and the lowest in 4.5 M DMSO with the CON method (15.17%). A dose-dependent increase in the proportion of oocytes fertilized was seen with increasing concentration of both EG and DMSO [CON: 4.5 M (15.2%), 5.5 M (25.6%); OPS: 4.5 M (21.3%), 5.5 M (27.5%)] in both CON and OPS methods. Comparison of the 2 cryoprotectants revealed that EG was better compared to DMSO.At equal concentrations of EG or DMSO, a significantly higher (P < 0.05) proportion of fertilized oocytes was seen in the OPS method compared to the CON method. It was concluded that vitrification results in some damage to oocytes, with decrease in their subsequent IVM and IVF. Developmental capacity of vitrified buffalo oocytes can be improved by using OPS instead of conventional straws.
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Cerna, Marco, Paulina Valdivieso, Rino Cella, Bence Mátyás, and Cristina Aucapiña. "Cryopreservation of orchid seeds through rapid and step freezing methods." F1000Research 7 (February 20, 2018): 209. http://dx.doi.org/10.12688/f1000research.13622.1.
Abstract:
Ecuador has a great variety of climatic regions that potentiate biodiversity. The family Orchidaceae constitutes one of the most important of the country, having identified about 4032 species with a high degree of endemism, therefore the development and research of alternative methods of storage and conservation of species is a strategy of primary interest for researchers and for society in general. In cryopreservation, temperatures reach below -190°C in order to paralyze the chemical reactions and keep the plant material viable for long periods. The present research focuses on the development of protocols for cryopreservation of seeds, aimed at the preservation of biodiversity, focusing on the family Orchidaceae, for the subsequent generation of a seed bank. The assays were performed on seeds of Epidendrum quitensium, Sobralia rosea, and Epidendrum anderssonii. Two freezing rates were tested: rapid freezing at -196°C; and step freezing at -22°C, -60°C to 196°C, further analyzed four combinations from Dimethylsulfoxide DMSO, glycerol and sucrose (DMSO 1M; DMSO 1M + glycerol 1M; DMSO 1M + sucrose 1M; DMSO 1M + glycerol 0,5M + sucrose 0,5M). The best results were obtained both in rapid and stepped freezing without the use of cryo-protective substances, by introducing the seeds directly into liquid nitrogen. Species of the genus Epidendrum presented a more efficient response in comparison to Sobralia. The viability of the seeds was evaluated by the tetrazolium test.
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Li, Yuebin, Lun Ma, Xing Zhang, Alan G. Joly, Zuli Liu, and Wei Chen. "Synthesis and Optical Properties of Sulfide Nanoparticles Prepared in Dimethylsulfoxide." Journal of Nanoscience and Nanotechnology 8, no. 11 (November 1, 2008): 5646–51. http://dx.doi.org/10.1166/jnn.2008.474.
Abstract:
Many methods have been reported for the formation of sulfide nanoparticles by the reaction of metallic salts with sulfide chemical sources in aqueous solutions or organic solvents. Here, we report the formation of sulfide nanoparticles in dimethylsulfoxide (DMSO) by boiling metallic salts without sulfide sources. The sulfide sources are generated from the boiling of DMSO and react with metallic salts to form sulfide nanoparticles. In this method DMSO functions as a solvent and a sulfide source as well as a stabilizer for the formation of the nanoparticles. The recipe is simple and economical making sulfide nanoparticles formed in this way readily available for many potential applications.
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Bohan, Azhar J., Duha S. Ahmed, and Ghaed K. Salman. "Synthesis AgNPs in Starch Solution by Green Method for New Medical Drugs." Materials Science Forum 1002 (July 2020): 311–18. http://dx.doi.org/10.4028/www.scientific.net/msf.1002.311.
Abstract:
Work represents the silver nanoparticles synthesis using green method by adding soluble starch in DMSO solution. The resulting AgNPs-based starch was noted via changing the color from yellow to brownish in aqueous DMSO solution in dark place due to the role of starch in the Ag ions reduction to AgNPs as capping agent in DMSO solvent at temperature 60°C. Then, the AgNPs development was characterized via the (UV-Vis) spectrophotometer. The morphology, structure and polydispersion of AgNPs were determined by using FESEM, XRD and Zeta analyzer, respectively which improved the role of starch in the formation of spherical and flower shapes of AgNPs. Moreover, the antibacterial activity of AgNPs-based starch revealed a strong effect against the positive bacteria than the negative bacteria as compared with the other antibiotic. Besides, these results suggest that the AgNPs prepared by green method can be utilized as efficient antimicrobial agent in the fields of medicine and represented the opportunities for the formation of safe and friendly AgNPs.
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Lima, Mariana da Cunha Lopes de, Celso Darío Ramos, Sérgio Quirino Brunetto, Marcelo Lopes de Lima, Ubirajara Ferreira, Elba Cristina Sá Camargo Etchebehere, Allan de Oliveira Santos, Nelson Rodrigues Netto Júnior, and Edwaldo Eduardo Camargo. "Estimation of absolute renal uptake with technetium-99m dimercaptosuccinic acid: direct comparison with the radioactivity of nephrectomy specimens." Sao Paulo Medical Journal 126, no. 3 (May 2008): 150–55. http://dx.doi.org/10.1590/s1516-31802008000300003.
Abstract:
CONTEXT AND OBJECTIVE: Studies using radionuclides are the most appropriate method for estimating renal function. Dimercaptosuccinic acid chelate labeled with technetium-99m (99mTc-DMSA) is the radiopharmaceutical of choice for high-resolution imaging of the renal cortex and estimation of the functional renal mass. The aim of this study was to evaluate a simplified method for determining the absolute renal uptake (ARU) of 99mTc-DMSA prior to nephrectomy, using the radioactivity counts of nephrectomy specimens as the gold standard. DESIGN AND SETTING: Prospective study at the Division of Nuclear Medicine, Department of Radiology, Universidade Estadual de Campinas. METHODS: Seventeen patients (12 females; range 22-82 years old; mean age 50.8 years old) underwent nephrectomy for various reasons. Renal scintigraphy was performed three to four hours after intravenous administration of a mean dose of 188.7 MBq (5.1 mCi) of 99mTc-DMSA, which was done six to 24 hours before surgery. The in vivo renal uptake of 99mTc-DMSA was determined using the radioactivity of the syringe before the injection (measured using a dose calibrator) and the images of the syringe and kidneys, obtained from a scintillation camera. After surgery, the reference value for renal uptake of 99mTc-DMSA was determined by measuring the radioactivity of the nephrectomy specimen using the same dose calibrator. RESULTS: The ARU measurements were very similar to those obtained using the reference method, as determined by linear regression (r-squared = 0.96). CONCLUSION: ARU estimation using the proposed method before nephrectomy seems to be accurate and feasible for routine use.
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Rakitin, Vladimir V., Pavel E. Varushkin, Hao Xin, and Gennady F. Novikov. "The use of liquid-phase method from DMSO solutions for synthesis of CZTS thin film materials." EPJ Photovoltaics 10 (2019): 6. http://dx.doi.org/10.1051/epjpv/2019007.
Abstract:
The possibility of synthesis of CZTS thin films on glass substrates from DMSO precursor solutions containing various quantity of copper ions was investigated. The dependence of composition of final CZTS compounds obtained from precursor solutions with different copper ion concentration in DMSO was shown. It was obtained that with the introduction of a low additive of copper ion concentration one can synthesize CZTS thin films with kesterite structure as well as with secondary phases (tin sulfides of SnS, Sn2S3). The increase of the concentration of copper ions in DMSO precursor solution promotes the formation of CZTS thin films with kesterite phase and the minimum content of impurities.
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Manolopoulou, E., Th Varzakas, and A. Petsalaki. "Chlorophyll Determination in Green Pepper Using two Different Extraction Methods." Current Research in Nutrition and Food Science Journal 4, no. 1 (March 9, 2015): 52–60. http://dx.doi.org/10.12944/crnfsj.4.special-issue1.05.
Abstract:
The aim of this paper is the comparison of the classic Arnon method with the DMSO method regarding the determination of chlorophyll content in California Wonder peppers stored for 25 days at 5, 10 and 20°C. The results suggest that the factors affecting chlorophyll degradation are temperature and storage time, as well as their interaction. There is a linear relationship between changes in chlorophyll content and storage temperature. The statistical analysis indicates that the two chlorophyll extraction methods considerably differ, as the chlorophyll contents obtained through the Arnon method were, in all cases, lower than those obtained through DMSO. It has to be stressed that the results greatly depend on both the selected solvent and the chlorophyll extraction method.
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Lee, Hyunjin, and Jun-Beom Park. "Dimethyl Sulfoxide Leads to Decreased Osteogenic Differentiation of Stem Cells Derived from Gingiva via Runx2 and Collagen I Expression." European Journal of Dentistry 13, no. 02 (May 2019): 131–36. http://dx.doi.org/10.1055/s-0039-1694904.
Abstract:
Abstract Objectives Dimethyl sulfoxide (DMSO) plays various functions, including cellular functions such as cellular growth. The aim of this study was to evaluate the effects of DMSO on the proliferation and osteogenic differentiation of human gingiva-derived stem cells. Materials and Methods Stem cells derived from gingiva were cultured in the presence of DMSO at concentrations ranging from 0.01 to 10%. Statistical Analysis We performed a one-way analysis of variance (ANOVA) with post hoc test to determine the differences between the groups using a commercially available program and the level of significance was 0.05. Results The cells in the control group showed normal fibroblast morphology. The cells treated with 0.01%, 0.01%, 0.1%, and 1% DMSO were morphologically similar to those from the control group on each day. Statistically significant decreases in cell counting kit-8 (CCK-8) values were seen in the 3% and 10% DMSO groups (p < 0.05). A statistically significant decrease in alkaline phosphatase activity was seen in the 3% DMSO group. (p < 0.05). The application of DMSO produced a decrease in alizarin red S staining. The expression of Runx2 and collagen I by immunofluorescence decreased as the dose of lovastatin increased. Conclusion The effects of DMSO on the viability of osteogenic differentiation among stem cells derived from human gingiva were evaluated. Applying DMSO produced decreased cell viability and decreased osteogenic differentiation in this experimental setting. This should be considered when designing and interpreting the data, and a DMSO-free method may be considered for bone regeneration applications.
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Băilă, Diana Irinel. "Experimental Researches of Co-Cr Alloys Powders Manufactured by Sintering Process DMLS and Ni-Cr Alloys Used in Dentistry." Advanced Materials Research 1119 (July 2015): 433–37. http://dx.doi.org/10.4028/www.scientific.net/amr.1119.433.
Abstract:
The purpose of this study was to evaluate the chemical and metallurgical aspects, mechanical properties and hardness of the Co-Cr alloy powders manufactured by sintering process DMLS and compared with Ni-Cr alloys used for personalized dental crowns.In dentistry are develop new methods to obtain personalized dental crowns quickly, the cost is not expensive and is identically with the dental scan realized by dentist. DMLS sintering manufacturing is a new method who permits to realize in the same day the dental crowns with the first control.Powders of Co-Cr alloys presents good sintered properties, good mechanical properties and the sintered probes don’t present tensile strength in the material after DMLS process.
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Ma, Jinji, Jinyu Guo, Safura Ahmad, Zhengqiang Li, and Jin Hong. "Constructing a New Inter-Calibration Method for DMSP-OLS and NPP-VIIRS Nighttime Light." Remote Sensing 12, no. 6 (March 13, 2020): 937. http://dx.doi.org/10.3390/rs12060937.
Abstract:
The anthropogenic nighttime light (NTL) data that are acquired by satellites can characterize the intensity of human activities on the ground. It has been widely used in urban development assessment, socioeconomic estimate, and other applications. However, currently, the two main sensors, Defense Meteorological Satellite Program’s Operational Linescan System (DMSP-OLS) and Suomi National Polar-orbiting Partnership Satellite’s Visible Infrared Imaging Radiometer Suite (NPP-VIIRS), provide inconsistent data. Hence, the application of NTL for long-term analysis is hampered. This study constructed a new inter-calibration method for DMSP-OLS and NPP-VIIRS nighttime light to solve this problem. First, NTL data were processed to obtain vicarious site across China. By comparing different candidate models, it is discovered the Biphasic Dose Response (BiDoseResp) model, which is a weighted combination of sigmoid functions, can best perform the regression between DMSP-OLS and logarithmically transformed NPP-VIIRS. The coefficient of determination of BiDoseResp model reaches 0.967. It’s residual sum of squares is 6.136 × 10 5 , which is less than 6.199 × 10 5 of Logistic function. After obtaining the BiDoseResp-calibrated VIIRS (BDRVIIRS), we smoothed it by a filter with optimal parameters to maximize the consistency. The result shows that the consistency of NTL data is greatly enhanced after calibration. In 2013, the correlation coefficient between DMSP-OLS and original NPP-VIIRS data in the China region is only 0.621, while that reaches to 0.949 after calibration. Finally, a consistent NTL dataset of China from 1992 to 2018 was produced. When compared with the existing methods, our method is applicable to the full dynamic range of DMSP-OLS. Besides, it is more suitable for country or larger scale areas. It is expected that this method can greatly facilitate the development of research that is based on the historical NTL archive.
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Çitoğlu, Senem, Özlem Duyar Coşkun, Le Duc Tung, Mehmet Ali Onur, and Nguyen Thi Kim Thanh. "DMSA-coated cubic iron oxide nanoparticles as potential therapeutic agents." Nanomedicine 16, no. 11 (May 2021): 925–41. http://dx.doi.org/10.2217/nnm-2020-0467.
Abstract:
Aim: Superparamagnetic cubic iron oxide nanoparticles (IONPs) were synthesized and functionalized with meso-2,3-dimercaptosuccinic acid (DMSA) as a potential agent for cancer treatment. Methods: Monodisperse cubic IONPs with a high value of saturation magnetization were synthesized by thermal decomposition method and functionalized with DMSA via ligand exchange reaction, and their cytotoxic effects on HeLa cells were investigated. Results: DMSA functionalized cubic IONPs with an edge length of 24.5 ± 1.9 nm had a specific absorption rate value of 197.4 W/gFe (15.95 kA/m and 488 kHz) and showed slight cytotoxicity on HeLa cells when incubated with 3.3 × 1010, 6.6 × 1010 and 9.9 × 1010 NP/mL for 24, 48 and 72 h. Conclusion: To the best of our knowledge, this is the first study to investigate both the cytotoxic effects of DMSA-coated cubic IONPs on HeLa cells and hyperthermia performance of these nanoparticles.
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BOJKO, Łukasz, Wojciech RYNIEWICZ, Anna M. RYNIEWICZ, and Marcin KOT. "STUDY OF THE IMPACT OF INCREMENTAL TECHNOLOGY ON MECHANICAL AND TRIBOLOGICAL PROPERTIES OF BIOMATERIALS." Tribologia 273, no. 3 (June 30, 2018): 29–38. http://dx.doi.org/10.5604/01.3001.0010.6117.
Abstract:
The latest method for creating denture replenishment in CAD / CAM systems is Direct Metal Laser Sintering (DMLS) technology. With the use of DMLS, prosthodontics, implant prosthetics, and facial-cranialjaw surgery adapted to individual patient conditions can be realized. The aim is to evaluate the strength, microstructure, and tribological properties of Ti6Al4V and CoCrMo alloys obtained from DMLS technology in the aspect of therapeutic constructions. The conducted tests show that, in the DMLS technology, as compared to milling technology preceded by casting and forging or pressed powder and sintering, for the same percentage composition of elements, the micromechanical properties, microstructural and tribological change. This procedure, from which constructions for various dental applications are obtained, is the new technology preferred for making permanent restorations faced with ceramics, producing intravascular implants, and implants of the temporomandibular joint. It can be an alternative to conventional cast-based methods and CAD / CAM based milling.
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Yu, Y. Y., R. Zhang, X. Q. Xiu, Z. L. Xie, H. Q. Yu, Y. Shi, B. Shen, S. L. Gu, and Y. D. Zheng. "Preparation GaxMn1−xN DMS materials using HVPE method." Journal of Crystal Growth 269, no. 2-4 (September 2004): 270–75. http://dx.doi.org/10.1016/j.jcrysgro.2004.05.086.
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Shrestha, Ojaswee, Nabina Shrestha, Sadhana Khanal, Sushant Pokhrel, Sujina Maharjan, Tika Bahadur Thapa, Puspa Raj Khanal, and Govardhan Joshi. "Inhibition and Reduction of Biofilm Production along with Their Antibiogram Pattern among Gram-Negative Clinical Isolates." International Journal of Biomaterials 2023 (November 17, 2023): 1–10. http://dx.doi.org/10.1155/2023/6619268.
Abstract:
Background. Bacterial biofilm is a significant virulence factor threatening patients, leading to chronic infections and economic burdens. Therefore, it is crucial to identify biofilm production, its inhibition, and reduction. In this study, we investigated biofilm production among Gram-negative isolates and assessed the inhibitory and reduction potential of ethylene diamine tetra acetic acid (EDTA) and dimethyl sulfoxide (DMSO) towards them. In addition, we studied the antimicrobial resistance pattern of the Gram-negative isolates. Methods. Bacterial isolation and identification was done using standard microbiological techniques, following the Clinical and Laboratory Standards Institute (CLSI) guideline, 28th edition. The Kirby–Bauer disk diffusion method was used to determine the antibiotic susceptibility pattern of the isolates, and β-lactamase production was tested via the combination disk method. Biofilm formation was detected through the tissue culture plate (TCP) method. Different concentrations of EDTA and DMSO were used to determine their inhibitory and reduction properties against the biofilm. Both inhibition and reduction by the various concentrations of EDTA and DMSO were analyzed using paired t-tests. Results. Among the 110 clinical isolates, 61.8% (68) were found to be multidrug resistant (MDR). 30% (33/110) of the isolates were extended-spectrum β-lactamase (ESBL) producers, 14.5% (16/110) were metallo-β-lactamase (MBL), and 8% (9/110) were Klebsiella pneumoniae carbapenemase (KPC) producers. Biofilm formation was detected in 35.4% of the isolates. Biofilm-producing organisms showed the highest resistance to antibiotics such as cephalosporins, chloramphenicol, gentamicin, and carbapenem. The inhibition and reduction of biofilm were significantly lower ( p < 0.05) for 1 mM of EDTA and 2% of DMSO. Conclusion. Isolates forming biofilm had a higher resistance rate and β-lactamase production compared to biofilm nonproducers. EDTA and DMSO were found to be potential antibiofilm agents. Hence, EDTA and DMSO might be an effective antibiofilm agent to control biofilm-associated infections.
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Trautmann, Laura, Attila Piros, János Péter Rádics, Ivett Jakab, and Katalin Badak-Kerti. "A new method for production development." Design of Machines and Structures 10, no. 2 (2020): 165–72. http://dx.doi.org/10.32972/dms.2020.029.
Abstract:
This paper introduces a new method for production development with the usage of process observation, fuzzy logic, and sensoring. In collaboration with experts in the field of food science, design, a program was developed that can simulate the behaviour of a semifinished product during preparation. A factory producing bakery products applied sensors in order to gain real-time data that can be integrated into the program. Because of all the uncertainty of a preparation process, the optimal baking temperature and time are different in each package of the product. With the previously mentioned program, it is possible to provide the optimal values for each package.
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Dvorak, Karel, Lucie Zarybnicka, and Jana Dvorakova. "Quality Parameters of 3D Print Products by the DMLS Method." Manufacturing Technology 19, no. 2 (April 1, 2019): 209–15. http://dx.doi.org/10.21062/ujep/271.2019/a/1213-2489/mt/19/2/209.
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Rokicki, Pawel, Bogdan Kozik, Grzegorz Budzik, Tomasz Dziubek, Jacek Bernaczek, Lukasz Przeszlowski, Olimpia Markowska, Bartlomiej Sobolewski, and Arkadiusz Rzucidlo. "Manufacturing of aircraft engine transmission gear with SLS (DMLS) method." Aircraft Engineering and Aerospace Technology 88, no. 3 (May 3, 2016): 397–403. http://dx.doi.org/10.1108/aeat-05-2015-0137.
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MANANDHAR, G., A. KHODJAKOV, and G. ONISHCHENKO. "Modified PEG-DMSO-serum method induces high rate of fusion." Cell Biology International Reports 14 (September 1990): 82. http://dx.doi.org/10.1016/0309-1651(90)90430-7.
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Chen, Miao, Bao Peng, and Liang Ma. "A New Security Localization Method for Detecting Malicious Beacon Nodes in Wireless Sensor Networks." Advanced Materials Research 186 (January 2011): 428–32. http://dx.doi.org/10.4028/www.scientific.net/amr.186.428.
Abstract:
In this paper, we address wireless sensor network localization problems that have high reliability in an environment where physical node destruction is possible. We propose a range-independent localization algorithm called security localization based on Detecting malicious beacon nodes (DMBSL) that allows sensors to passively determine their location with high reliability, without increasing the number of reference points, or the complexity of the hardware of each reference point or node. In DMBSL, constraints of wireless sensor network are used to find and remove the malicious beacon nodes, then the maximum likelihood method is used to calculate the location of unknown nodes, so that the location calculation is very robust and is able to resist malicious attacks. In this paper, the location performance of DMBSL algorithm is deeply analyzed. The results of the simulation show the algorithm can get lower average positioning error, meantime malicious attacks have little side effects to location performance.
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Harika, Chembeti, K. Mahendranadh Reddy, and Venkat Adithya. "Comparison of fatigue behaviour and fracture toughness of cobalt chromium alloy fabricated by casting and selective laser melting methods." IP Annals of Prosthodontics and Restorative Dentistry 9, no. 3 (October 15, 2023): 158–64. http://dx.doi.org/10.18231/j.aprd.2023.031.
Abstract:
Co-Cr alloys are most commonly used base metal alloy in dentistry for the fabrication of many types of metallic substructures. They gained popularity due to their good mechanical properties and low cost. There are different techniques for fabrication of Co-Cr alloys which includes casting and DMLS technique. However, change in the fabrication techniques showed variation in mechanical properties. Forty Co-Cr samples each were made from two different techniques, Casting and DMLS. Fracture toughness was done with charpy’s impact test machine and fatigue behaviour test was done with nano universal testing machine. The samples were later observed under stereo electron microscope to assess the kind of failure. Unpaired t test revealed that there was statistically significant difference between two different groups. The mean difference of fracture toughness in Casting group was higher when compared with DMLS group. The number of fatigue cycles were higher in DMLS group than the casting group. Within the limitations of this in vitro study, casting group showed higher fracture toughness when compared to DMLS group, whereas fatigue limit was higher in DMLS group than casting group.
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Song, Chen, Elena Castellanos-Rizaldos, Rafael Bejar, Benjamin L. Ebert, and G. Mike Makrigiorgos. "DMSO Increases Mutation Scanning Detection Sensitivity of High-Resolution Melting in Clinical Samples." Clinical Chemistry 61, no. 11 (November 1, 2015): 1354–62. http://dx.doi.org/10.1373/clinchem.2015.245357.
Abstract:
Abstract BACKGROUND Mutation scanning provides the simplest, lowest-cost method for identifying DNA variations on single PCR amplicons, and it may be performed before sequencing to avoid screening of noninformative wild-type samples. High-resolution melting (HRM) is the most commonly used method for mutation scanning. With PCR-HRM, however, mutations less abundant than approximately 3%–10% that can still be clinically significant may often be missed. Therefore, enhancing HRM detection sensitivity is important for mutation scanning and its clinical application. METHODS We used serial dilution of cell lines containing the TP53 exon 8 mutation to demonstrate the improvement in detection sensitivity for conventional-PCR-HRM in the presence of DMSO. We also conducted coamplification at lower denaturation temperature (COLD)-PCR with an extra step for cross-hybridization, followed by preferential denaturation and amplification at optimized critical temperature (full-COLD-PCR), to further enrich low-level mutations before HRM with or without DMSO, and we used droplet-digital PCR to derive the optimal conditions for mutation enrichment. Both conventional PCR-HRM and full-COLD-PCR-HRM with and without DMSO were used for mutation scanning of TP53 exon 8 in cancer samples containing known mutations and myelodysplastic syndrome samples with unknown mutations. Mutations in other genes were also examined. RESULTS The detection sensitivity of PCR-HRM scanning increases 2- to 5-fold in the presence of DMSO, depending on mutation type and sequence context, and can typically detect mutation abundance of approximately 1%. When mutation enrichment is applied during amplification with full-COLD-PCR followed by HRM in the presence of DMSO, mutations with 0.2%–0.3% abundance in TP53 exon 8 can be detected. CONCLUSIONS DMSO improves HRM mutation scanning sensitivity with saturating dyes. When full-COLD-PCR is used, followed by DMSO-HRM, the overall improvement is about 20-fold compared with conventional PCR-HRM.
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Yushkin, Alexey A., Alexey V. Balynin, Mikhail E. Efimov, Galina Karpacheva, and Alexey V. Volkov. "Preparation of Fine Porous Ultrafiltration Membranes from Polyacrylonitrile." Key Engineering Materials 869 (October 2020): 437–42. http://dx.doi.org/10.4028/www.scientific.net/kem.869.437.
Abstract:
PAN membranes were prepared from mixture of good and weak solvents by two different methods. Immersion precipitation method with and without volatile component evaporation used for membrane preparation. From analysis of Hansen solubility parameters DMSO chosen as good solvent and acetone chosen as weak solvent. The effect of volatile weak solvent investigated on pore size and filtration performance. By evaporation of acetone, it was possible to increase polymer concentration on casting solution and obtain smaller pore size in comparison to membrane prepared from DMSO. Membranes prepared from DMSO/acetone without solvent evaporation had even lower pore size and higher filtration performances. It was shown, that presence of acetone creates more pronounced effect on pore size than increase of polymer concentration. As a result membranes with pore size 3.7 nm obtained from Acetone/DMSO mixture with composition 50:50 and polymer concentration 50 % by immersion precipitation Resulted membranes have permeance 23 kg/m2·h·bar and retention of Lysozime 99 %.
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Karolewska, Karolina, Bogdan Ligaj, and Dariusz Boroński. "Strain Analysis of Ti6Al4V Titanium Alloy Samples Using Digital Image Correlation." Materials 13, no. 15 (July 31, 2020): 3398. http://dx.doi.org/10.3390/ma13153398.
Abstract:
Digital image correlation (DIC) is a non-contact optical method that allows measuring displacements on a plane used to determine the strains caused by external loads of a structural element (mechanical or thermal). Currently, digital image correlation is a widely used experimental technique to assess the mechanical behavior of materials, in particular cracking characteristics and destruction methods of various structural elements. In this paper, the DIC method is applied to determine local strains of titanium alloy Ti6Al4V specimen. The samples used in the tests were made with two different technologies: (a) from a drawn bar by machining process; and (b) by the additive manufacturing method Direct Metal Laser Sintering (DMLS). The aim of the paper is to present the mechanical properties test results of the Ti6Al4V titanium alloy produced by the DMLS additive manufacturing under static loads using the digital image correlation method. As a result of the tests carried out on the drawn bar specimens, it was concluded that the change in the measurement base affects the difference in the Young’s E modulus value in the range from 89.2 to 103.8 GPa. However, for samples formed using the DMLS method, the change in the Young’s modulus value was from 112.9 to 115.3 GPa for the same measurement base.
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Akter, Farzana, Md Nure Alam Abdullah, M. Abdul Momin, and M. Selim Reza. "Relative renal functions for children using 99mTC-DTPA and 99mTC-DMSA." Journal of Bangladesh Academy of Sciences 44, no. 2 (January 19, 2021): 109–19. http://dx.doi.org/10.3329/jbas.v44i2.51455.
Abstract:
Radionuclides are widely used to study the relative renal functions (RRFs) for kidney patients. This work reports the determination of the RRFs using the technetium-99m dimercaptosuccinic acid (99mTc-DMSA) and technetium-99m diethylenetriamine-pentaacetic acid (99mTc-DTPA) for children having kidney diseases. The two methods are then compared to find the choice of scintigraphy for a particular type of disease. A total of 65 child kidney patients of ages 5 months - 16 years were the representative samples of the study. The mean RRFs calculated with 99mTc-DTPA was found as and respectively, for the left and right kidneys. On the other hand, the values are and respectively, using 99mTc-DMSA. a significant positive correlation () were found between the RRFs calculated with the two scanning methods in Pearson correlation analysis. In one way ANOVA test, we did not find any difference between the RRFs calculated with 99mTc-DTPA and 99mTc-DMSA between the left and right kidneys. In Bland-Altman Plots, no mean difference was found between the two scanning methods and the correlation limit lies between -13.5 and12.6. The present study suggests using a single method for a particular type of disease. 99mTc-DMSA is the primary choice for the evaluation of RRF since it is easy, inexpensive and also provides cortical morphology, renal scar and cyst evaluation. But for glomerular filtration rate and renogram curve, 99mTc-DTPA should be the obvious selection as 99mTc-DMSA fails to provide any information for these cases.
Journal of Bangladesh Academy of Sciences, Vol. 44, No. 2, 109-119, 2020
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Martin, W. Blake, Renaud Sicard, Shabnam M. Namin, and Timothy Ganey. "Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects." BioMed Research International 2019 (October 16, 2019): 1–7. http://dx.doi.org/10.1155/2019/5025398.
Abstract:
Debridement of the bone surface during a surgical fusion procedure initiates an injury response promoting a healing cascade of molecular mediators released over time. Autologous grafts offer natural scaffolding to fill the bone void and to provide local bone cells. Commercial bone grafting products such as allografts, synthetic bone mineral products, etc., are used to supplement or to replace autologous grafts by supporting osteoinductivity, osteoconductivity, and osteogenesis at the surgical site. To assure osteogenic potential, preservation of allogeneic cells with cryoprotectants has been developed to allow for long-term storage and thus delivery of viable bone cells to the surgical site. Dimethyl sulfoxide (DMSO) is an intracellular cryoprotectant commonly used because it provides good viability of the cells post-thaw. However, there is known cytotoxicity reported for DMSO when cells are stored above cryogenic temperatures. For most cellular bone graft products, the cryoprotectant is incorporated with the cells into the other mineralized bone and demineralized bone components. During thawing, the DMSO may not be sufficiently removed from allograft products compared to its use in a cell suspension where removal by washing and centrifugation is available. Therefore, both the allogeneic cell types in the bone grafting product and the local cell types at the bone grafting site could be affected as cytotoxicity varies by cell type and by DMSO content according to reported studies. Overcoming cytotoxicity may be an additional challenge in the formation of bone at a wound or surgical site. Other extracellular cryoprotectants have been explored as alternatives to DMSO which preserve without entering the cell membrane, thereby providing good cellular viability post-thaw and might abrogate the cytotoxicity concerns.
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Ma, Chunqing, Ming-Fai Lo, and Chun-Sing Lee. "A simple method for phase control in two-dimensional perovskite solar cells." Journal of Materials Chemistry A 6, no. 39 (2018): 18871–76. http://dx.doi.org/10.1039/c8ta06976j.
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Kuwajima, Kunihiro, Maho Yagi-Utsumi, Saeko Yanaka, and Koichi Kato. "DMSO-Quenched H/D-Exchange 2D NMR Spectroscopy and Its Applications in Protein Science." Molecules 27, no. 12 (June 10, 2022): 3748. http://dx.doi.org/10.3390/molecules27123748.
Abstract:
Hydrogen/deuterium (H/D) exchange combined with two-dimensional (2D) NMR spectroscopy has been widely used for studying the structure, stability, and dynamics of proteins. When we apply the H/D-exchange method to investigate non-native states of proteins such as equilibrium and kinetic folding intermediates, H/D-exchange quenching techniques are indispensable, because the exchange reaction is usually too fast to follow by 2D NMR. In this article, we will describe the dimethylsulfoxide (DMSO)-quenched H/D-exchange method and its applications in protein science. In this method, the H/D-exchange buffer is replaced by an aprotic DMSO solution, which quenches the exchange reaction. We have improved the DMSO-quenched method by using spin desalting columns, which are used for medium exchange from the H/D-exchange buffer to the DMSO solution. This improvement has allowed us to monitor the H/D exchange of proteins at a high concentration of salts or denaturants. We describe methodological details of the improved DMSO-quenched method and present a case study using the improved method on the H/D-exchange behavior of unfolded human ubiquitin in 6 M guanidinium chloride.