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Dissertations / Theses on the topic 'Displacement amplification'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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1

Brown, Daniel F. Jr. "Single crystal piezoelectric pumping using displacement amplification." Thesis, Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/19124.

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2

Morant, Nick. "Novel thermostable DNA polymerases for isothermal DNA amplification." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667735.

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DNA polymerases play a fundamental role in the transmission and maintenance of genetic information and have become an important in vitro diagnostic and analytical tool. The Loop-mediated isothermal DNA amplification (LAMP) method has major applications for disease and pathogen detection and utilises the unique strand-displacement activity of a small group of thermostable DNA polymerases. The Large (Klenow-like) Fragment of Geobacillus stearothermophilus DNA polymerase I (B.st LF Pol I) currently serves as the enzyme of choice for the majority of these isothermal reactions, with few alternative
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3

Anscombe, C. J. "Multiple displacement amplification and whole genome sequencing for the diagnosis of infectious diseases." Thesis, Queen Mary, University of London, 2016. http://qmro.qmul.ac.uk/xmlui/handle/123456789/18409.

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Next-generation sequencing technologies are revolutionising our ability to characterise and investigate infectious diseases. Utilising the power of high throughput sequencing, this study reports, the development of a sensitive, non-PCR based, unbiased amplification method, which allows the rapid and accurate sequencing of multiple microbial pathogens directly from clinical samples. The method employs Φ29 DNA polymerase, a highly efficient enzyme able to produce strand displacement during the polymerisation process with high fidelity. Problems with DNA secondary structure were overcome and the
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4

Thornley, John K. "Methods of application of piezoelectric multilayer actuators to high-speed clutching, using displacement amplification." Thesis, Loughborough University, 1993. https://dspace.lboro.ac.uk/2134/10851.

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The suitability of electromagnetic actuating devices for application to machines with ever more demanding response time specifications is discussed, with the proposal that piezoelectric actuator technology can produce practical devices with faster response times than solenoids, for example. This thesis discusses and validates the proposition that the performance of piezoelectric ceramic actuators makes them viable devices for inclusion in high-speed machine applications, where rapid clutching using two-state actuation is required. Further, techniques are devised and explored for the design and
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5

Muharam, Firman Alamsyah. "Overcoming problems with limiting DNA samples in forensics and clinical diagnostics using multiple displacement amplification." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16207/.

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The availability of DNA samples that are of adequate quality and quantity is essential for any genetic analysis. The fields of forensic biology and clinical diagnostic pathology testing often suffer from limited samples that yield insufficient DNA material to allow extensive analysis. This study examined the utility of a recently introduced whole genome amplification method termed Multiple Displacement Amplification (MDA) for amplifying a variety of limited sample types that are commonly encountered in the fields of forensic biology and clinical diagnostics. The MDA reaction, which employs the
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6

Akinbami, Omolola Adetola. "Use of multiple displacement amplification based approaches for detection and analysis of environmentally significant and contaminating bacteria in fresh water." Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602407.

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One of the great challenges of microbial analysis in natural environments derives from the fact that a large proportion of microorganisms present is not culturable in standard conditions. To overcome this, various molecular approaches are widely used. Multiple Displacement Amplification (MDA) is especially useful as it can be applied in conjunction with other techniques to identify genes derived from individual microbial cells. The aim of this project was to study freshwater samples obtained from various environments in Ireland in order to identify dominant bacterial species and key genes asso
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7

Rönn, Ann-Charlotte. "Analysis of Nucleotide Variations in Non-human Primates." Doctoral thesis, Uppsala University, Molecular Medicine, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7904.

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<p>Many of our closest relatives, the primates, are endangered and could be extinct in a near future. To increase the knowledge of non-human primate genomes, and at the same time acquire information on our own genomic evolution, studies using high-throughput technologies are applied, which raises the demand for large amounts of high quality DNA.</p><p>In study I and II, we evaluated the multiple displacement amplification (MDA) technique, a whole genome amplification method, on a wide range of DNA sources, such as blood, hair and semen, by comparing MDA products to genomic DNA as templates for
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8

Brito, Luciana Carla Neves. "Análise microbiológica de infecções endodônticas utilizando a associação das técnicas do Multiple Displacement Amplification (MDA) e da Hibridização DNA-DNA (Checkerboard)." Universidade Federal de Minas Gerais, 2007. http://hdl.handle.net/1843/ZMRO-77LM2N.

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Multiple Displacement Amplification (MDA) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular analysis. The purpose of this investigation was to combine MDA and checkerboard DNA-DNA hybridization to examine the microbiota of endodontic infections. 66 samples were collected from teeth with endodontic infections. Non-amplified and MDA amplified samples were analyzed by checkerboard DNA-DNA hybridization for levels and proportions of 77 bacterial taxa. Counts, % DNA probe counts and % of teeth colonized for each species in amplif
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9

Halilovic, Amina. "SÄKERSTÄLLNING AV SÄLLSYNTA DNA-KONTROLLER MED HELGENOMAMPLIFIERING I KLINISKT SYFTE." Thesis, Malmö högskola, Fakulteten för hälsa och samhälle (HS), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-24387.

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Vid klinisk enbaspolymorfi (SNP) analys inkluderas DNA-kontroller med kända genotyper i varje analysomgång för att säkerställa riktigheten vad gäller analysresultatet. DNA-kontrollerna har en central roll för resultatens trovärdighet vid genotypningen. Vissa kontrollprover som används är av sällsynt genotyp och kan vara mycket svåra att få tag på. Detta arbete har utförts för att undersöka om det går att erhålla DNA-material från sällsynta genotyper med hjälp av helgenomamplifiering och på så sätt säkerställa en tillgång till dessa. I arbetet testades helgenomamplifiering med hjälp av två olik
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10

Yazgan, Ufuk. "A Methodology For Determination Of Performance Based Design Parameters." Master's thesis, METU, 2003. http://etd.lib.metu.edu.tr/upload/4/1120682/index.pdf.

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Establishment of relationships for predicting the lateral drift demands of near-fault ground motions is one of the major challenges in earthquake engineering. Excessive lateral drifts caused by earthquake ground motions are the major causes of structural damage observed in structures. In this study, some of the fundamental characteristics of near-fault ground motions are examined. Response characteristics of elastic frame structures to near-fault ground motions are investigated. An approximate method for estimating the elastic ground story and interstory drifts for regular frame type structure
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11

Tavares, Warley Luciano Fonseca. "Estudo das comunidades microbianas associadas às infecções endodônticas de dentes decíduos sintomáticos e assintomáticos pelas técnicas do Multiple-Displacement Amplification e Checkerboard DNA-DNA Hybridization." Universidade Federal de Minas Gerais, 2008. http://hdl.handle.net/1843/BUOS-9K2GUG.

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Multiple Displacement Amplification (MDA) has been used to uniformly amplify the genome from bacterial species in different types of oral samples. MDA is particularly useful in small samples, since it generates abundant target for microbial analysis. The aim of the present study was to combine MDA and Checkerboard DNA-DNA hybridization to evaluate the microbiota of endodontic infections in deciduous teeth A total of 35 children, 4 to 10 years old, having teeth with intact roots or less than 2/3 of physiological root resorption were involved in this study. Forty root canal samples were collecte
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12

Kurban, Can Ozan. "A Numerical Study On Response Factors For Steel Plate Shear Wall Systems." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610741/index.pdf.

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Design recommendations for steel plate shear wall (SPSW) systems have recently been introduced into seismic provisions for steel buildings. Response modification, overstrength, and displacement amplification factors for SPSW systems presented in the design codes were based on professional experience and judgment. A numerical study has been undertaken to evaluate these factors for SPSW systems. Forty four unstiffened SPSWs possessing different geometrical characteristics were designed based on the recommendations given in the AISC Seismic Provisions. Bay width, number of stories, story mass,
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13

Raikar, Sanjeev Vencu. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080214.105406/.

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Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression by Sanjeev V. Raikar Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two
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14

Fatemi, Hassan. "Investigation of the higher mode effects on the dynamic behaviour of reinforced concrete shear walls through a pseudo-dynamic hybrid test." Thèse, Université de Sherbrooke, 2017. http://hdl.handle.net/11143/11221.

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La plupart des bâtiments de moyenne et grande hauteur en béton armé sont munis de murs de refend ductiles afin résister aux charges latérales dues au vent et aux séismes. Les murs de refend ductiles sont conçus selon des règles conception stricts. Ces murs sont généralement conçus de façon à forcer la formation d’une rotule plastique à leur base dans l’éventualité d’un séismemajeur. Lors de la conception d’un mur, l’enveloppe des moments fléchissants ainsi que l’enveloppe des efforts tranchants dans la portion du mur situé au-dessus de la rotule plastique sont basés sur la résistance pro
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15

Dussutour, Audrey. "Organisation spatio-temporelle des déplacements collectifs chez les fourmis." Toulouse 3, 2004. http://www.theses.fr/2004TOU30242.

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This study deals with the organisation of collective movements in ants in presence of environmental heterogeneities, particularly in situations involving crowding. We chose two species of ants, characterised by different degrees of polymorphism, as well as by their mode of food transport. Our aim is to identify the link between the behavioural rules observed at the scale of the individual and the spatio-temporal organisation observed at the scale of the group. Ndependently of the species, we found that the regulation of traffic in crowding situations depends both on interattraction processes,
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16

Raikar, S. V. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Diss., Lincoln University, 2007. http://hdl.handle.net/10182/301.

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Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two species. This thesis describes the somatic hybridisation, the regeneration and the molecular analysis
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17

Benmohamed, Fayçal. "Contribution au développement d'une méthode numérique pour étudier des structures planaires hyperfréquences." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSES004.

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Le travail proposé est le résultat d’une collaboration entre le laboratoire Physique de la matière condensée et nanosciences de Monastir, le laboratoire Laplace de Toulouse et le laboratoire Hubert Curien de Saint-Étienne. Il s’inscrit dans un projet visant à étudier théoriquement la propagation des ondes électromagnétiques dans des dispositifs passifs à base du matériau magnétique anisotrope. La présence d’une couche magnétique dans telle structure a de nombreux avantages : non réciprocité́, performances intéressantes, isolation importante, …Plus précisément, c’est la fabrication des disp
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18

Singh, Mandeep. "STUDIES ON THE SIZE AND NON-PLANARITY OF AROMATIC STACKING MOIETY ON CONFORMATION SELECTIVITY AND THERMAL STABILIZATION OF G-QUADRUPLEXES." Scholarly Commons, 2020. https://scholarlycommons.pacific.edu/uop_etds/3725.

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Targeting DNA has the advantage over proteins for cancer remediation because of the fewer copies of the ligands required for the desired therapeutic effect. Traditionally, covalent DNA binders like alkylating agents have been used to induce genetic instability through the formation of DNA lesions and strand breaks, leading to cellular apoptosis. The primary drawback of this treatment is the non-specific binding that affects both cancerous and non-cancerous cells. G-quadruplexes are the DNA secondary structures that are present in abundance near the promoter regions of the oncogenes and are inv
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19

Chang, Li-Liang, and 張立良. "Study on a Micro Feeding Tool Holder with Hydraulic Displacement Amplification Mechanism." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/41780208292232632815.

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碩士<br>國立高雄第一科技大學<br>機械與自動化工程研究所<br>99<br>In this study, a micro feeding tool holder with a displacement magnification mechanism is provided for improving the feeding resolution and providing tool-servo function of an existing ultraprecision machine. The feeding stroke of the tool holder is amplified by a bellows-type hydraulic device with two different cross-sectional areas. Due to the nature of incompressible liquid in the closed chamber of the hydraulic device, the tiny displacement of the PZT actuator can be amplified based on the area ratio of the two bellows. In this paper, detail design
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20

Phung, Van-Bang, and 馮文龐. "Amplification factors for spectral acceleration, velocity, and displacement for the1999 Chi-Chi earthquake." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/41844132553439631826.

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碩士<br>國立臺灣大學<br>土木工程學研究所<br>100<br>In this study, 838 ground motion records from the 1999 Chi-Chi earthquake in Taiwan are used to compute the amplification factors between average spectral acceleration (velocity, displacement) in acceleration (velocity, displacement) sensitive region and peak ground acceleration (velocity, displacement). The results are compared with those presented in literatures. This study also examines the impact of site condition (VS30), closest site-to-source distance and hanging/foot-wall effect on amplification factors. The study shows that (1) among the amplification
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21

Sie, Qing-Yu, and 謝謦羽. "Optimization Design of a Compliant Displacement Amplification Mechanism Using Taguchi Method Based Grey Relational Analysis." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/u9t44d.

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碩士<br>國立高雄應用科技大學<br>機械工程系<br>106<br>In this thesis, Taguchi quality design is applied and the gray relational method is used to explore the optimal design of the hinge of flexible mechanism applied in the displacement magnification mechanism. In the study, three models of flexible hinges and displacement amplifying mechanisms with different geometric shapes were established by using SolidWorks drawing software, and an appropriate force was applied as a force to simulate the finite element analysis software ANSYS Workbench and find out through Taguchi quality design. The best combination of the
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22

Chang, Wen-Hsin, and 張文馨. "DNA-based Hydrogel Microcapsules Coupled with Strand-Displacement Amplification for the Detection of miR-141." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/w666xb.

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23

Schoenborn, Veit. "Whole Genome Amplification von Plasma-DNA und Entwicklung eines Ausschlusskriteriums zur Verbesserung der Genotypisierungsqualität." Doctoral thesis, 2008. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-37136.

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Plasma- und Serumproben waren in früheren epidemiologischen Studien häufig das einzige biologische Material, das gesammelt und untersucht wurde. Diese Studien besitzen gerade durch ihren sehr langen Untersuchungszeitraum einen riesigen Informationsgehalt und wären ein unbezahlbarer Schatz für genetische Analysen. Oft ist aufgrund damals mangelnder Akquirierung jedoch keine genomische DNA verfügbar. Um die in Plasmaproben in geringer Menge vorkommende DNA verwenden zu können, extrahierten wir die DNA mit Hilfe von magnetischen Partikeln und setzten sie in eine Whole Genome Amplification (WGA) m
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24

Peng, Wen-Fei, and 彭文飛. "Modeling the Earthquake-Induced Landslide Hazard Assessment Based on the Cumulative Displacement Method Incorporating Topographic Amplification and Sliding Area Effects." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/75510257845714519671.

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博士<br>國立成功大學<br>資源工程學系碩博士班<br>96<br>A procedure that considers topographic effects and runout behavior is proposed for analyzing seismic landslide hazards. The theoretical topographic amplification factors and corresponding amplified ground motion are calculated. By using the amplified motion, a cumulative displacement map is generated through Newmark’s displacement method. The high displacement areas are defined as the source areas of landslides. A runout simulation that identifies sliding routes and the final deposition areas of the sliding material from these source areas is performed. Fina
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25

Wang, Shih-Ting, and 王士庭. "Study on the strength reduction factor, yielding strength factor, and displacement amplification factor for structures subjected to near-fault earthquake." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/p8n5nz.

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碩士<br>國立交通大學<br>土木工程系所<br>106<br>During the Chi-Chi earthquake in Taiwan, it was observed that the near-fault ground motions with pulse-like signals caused some serious damages to bridge structures. This study proposes revised engineering design parameters including strength reduction factor, R, yielding strength factor, Cy, displacement amplification factor, Rd, which could provide suitable design solutions to minimize damages to bridge structures during near-fault earthquake motions. The analysis involved collection of related seismic records, focusing on the aforementioned factors. Using th
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26

Verastegui, Pena Yris Milusqui. "Targeting novel soil glycosyl hydrolases by combining stable isotope probing and metagenomics." Thesis, 2014. http://hdl.handle.net/10012/8282.

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Soil represents the largest global reservoir of microbial diversity for the discovery of novel genes and enzymes. Both stable-isotope probing (SIP) and metagenomics have been used to access uncultured microbial diversity, but few studies have combined these two methods for accessing the biotechnological potential of soil genetic diversity and fewer yet have employed functional metagenomics for recovering novel genes and enzymes for bioenergy or bioproduct applications. In this research, I demonstrate the power of combining functional metagenomics and SIP using multiple plant-derived carbon sub
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