Academic literature on the topic 'Direct sequencing'
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Journal articles on the topic "Direct sequencing"
Ozsolak, Fatih, Adam R. Platt, Dan R. Jones, Jeffrey G. Reifenberger, Lauryn E. Sass, Peter McInerney, John F. Thompson, Jayson Bowers, Mirna Jarosz, and Patrice M. Milos. "Direct RNA sequencing." Nature 461, no. 7265 (September 23, 2009): 814–18. http://dx.doi.org/10.1038/nature08390.
Full textLinder, Jodell E., Tatyana E. Plachco, Romina Libster, and E. Kathryn Miller. "Sequencing human rhinoviruses: Direct sequencing versus plasmid cloning." Journal of Virological Methods 211 (January 2015): 64–69. http://dx.doi.org/10.1016/j.jviromet.2014.09.020.
Full textChalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (August 24, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.2.
Full textChalapati, Sachin, Conor A. Crosbie, Dixita Limbachiya, and Nimesh Pinnamaneni. "Direct oligonucleotide sequencing with nanopores." Open Research Europe 1 (May 12, 2021): 47. http://dx.doi.org/10.12688/openreseurope.13578.1.
Full textRudi, Heidi, Knut‐Erik Gylder, Odd Arne Rognli, and Knut Rudi. "Direct Haplotype‐Specific DNA Sequencing." Preparative Biochemistry and Biotechnology 36, no. 3 (September 2006): 253–57. http://dx.doi.org/10.1080/10826060600716687.
Full textZhang, Jinyue, Shuanghong Yan, Le Chang, Weiming Guo, Yuqin Wang, Yu Wang, Panke Zhang, Hong-Yuan Chen, and Shuo Huang. "Direct microRNA Sequencing Using Nanopore-Induced Phase-Shift Sequencing." iScience 23, no. 3 (March 2020): 100916. http://dx.doi.org/10.1016/j.isci.2020.100916.
Full textKilger, Christian, Matthias Krings, Hendrik Poinar, and Svante Pääbo. "“Colony Sequencing”: Direct Sequencing of Plasmid DNA from Bacterial Colonies." BioTechniques 22, no. 3 (March 1997): 412–18. http://dx.doi.org/10.2144/97223bm08.
Full textPetry, H., K. Pekrun, K. Wäse, I. Schedel, W. Lüke, and G. Hunsmann. "Direct sequencing versus cloned amplicon sequencing in HIV-1 diagnosis." Experientia 52, no. 4 (April 1996): 303–4. http://dx.doi.org/10.1007/bf01919520.
Full textObata, Hiroko, Tatsuya Tanaka, Tsuneko Fujii, Chikako Sasho, Yukihiro Yamaguchi, and Keiichiro Suzuki. "Dye Terminator Re-cycle-sequencing Method: Phage Plaque Direct Sequencing." Analytical Biochemistry 297, no. 1 (October 2001): 102–5. http://dx.doi.org/10.1006/abio.2001.5328.
Full textZhang, Jinyue. "Direct MicroRNA Sequencing using Nanopore Induced Phase-Shift Sequencing (NIPSS)." Biophysical Journal 118, no. 3 (February 2020): 475a—476a. http://dx.doi.org/10.1016/j.bpj.2019.11.2638.
Full textDissertations / Theses on the topic "Direct sequencing"
Fillion, Bergeron Marianne. "Plasmid isolation and purification by electrofiltration and comparison of different direct colony sequencing methods and PCR-based sequencing methods." Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6050.
Full text梁秀敏 and Sau-man Leung. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970102.
Full textLeung, Sau-man. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2329498X.
Full textOtten, Nathalie. "DNA of bovine papillomavirus type 1 and 2 in equine sarcoids : PCR detection and direct sequencing /." [S.l.] : [s.n.], 1992. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Full textRocha, Ana Laís Bignotto da. "Sequenciamento direto dos genes SIX3, SHH, TGIF1, ZIC2 e array-CGH no estudo de pacientes com holoprosencefalia." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-12112013-150520/.
Full textObjective: Analyze through direct sequencing technique the presence of molecular changes on the genes SHH, SIX3, ZIC2 and TGIF1 on individuals with clinical diagnosis of HPE. Analyze through array-CGH technique the presence of molecular changes on individuals with clinical diagnosis of HPE previously submitted to the direct sequencing analyzes. Local: Genetics and Human Cytogenetics Laboratory, HRAC/USP, Bauru-SP. Methods: Were selected 50 individuals from both genders with ages between 03 months and 50 years clinically diagnosed with HPE. Everyone was analyzed through the direct sequencing technique for the genes SHH and TGIF1 completely and for the genes ZIC2 and SIX3 partially. From those individuals which did not have shown changes on the direct sequencing technique, eight individuals with more severe phenotype were selected to the analysis through array-CGH. Results an Discussion: Were analyzed 50 individuals through the technique of direct sequencing of the genes SHH and TGIF1, were found two pathogenic variants in the analysis of SHH gene, in the case 1, the variant p.G24P was identified, and in the case 2 was identified the variant c.1031delC. On the TGIF1 gene were found five polymorphisms already described on the literature. Was identified a new silent variant on the exon 1 of the ZIC2 gene p. Q46Q(c.431G>A) and a polymorphism already described in the literature in two individuals on the gene SIX3. The analysis through array-CGH revealed the presence of one microdeletion in the case 37, of 1,5 Mb on the region 17p12 between the genomic positions 14,052,279-15,102,307. The same deletion was detected in the mother, though this region was never associated to the HPE. Conclusion: The direct sequencing technique is a very important tool for the molecular diagnosis of the HPE, and the direct sequencing standardization for the genes ZIC2 and SIX3 might help in more precise diagnostics on HRAC/USP future studies. The employ of new techniques such as array-CGH may indicate new relations between chromosomal regions and the multiple hit involved in the development of HPE.
Vander, Wiede Jamie. "Direct, hands-on or inquiry instruction a study of instructional sequencing and motivation in the science classroom." Master's thesis, University of Central Florida, 2011. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5073.
Full textID: 030423060; System requirements: World Wide Web browser and PDF reader.; Mode of access: World Wide Web.; Thesis (M.A.)--University of Central Florida, 2011.; Includes bibliographical references (p. 42-48).
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Koziol, Adam. "Application of Direct-sequencing Peptide Proteomics to the Characterization of Antagonistic (Endogenous and Exogenous) Proteins in Cereal Grains." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/23853.
Full textNguyen, Thuy. "Ultra-deep sequencing applications in virology research." Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS282.
Full textThe two RNA viruses HIV and HCV are getting a lot of public health concerns because both of them have overlapping risk factors for transmission through direct blood and sexual contacts. Furthermore, HIV and HCV infections are the leading cause of mortality and morbidity globally due to related diseases. However, with the introduction of antiretroviral therapy (ART) for the treatment of HIV infection and direct-acting antivirals (DAAs) for the treatment of HCV infection, patients infected by these viruses are witnessing significant improvement in their quality of life. However, the high replication rate and the lack of error correction mechanism of these viruses result in a diverse viral population referred to as quasispecies. Under drug- selective pressure, the viral quasispecies select resistance variants against corresponding drug and render the therapy ineffective especially in cases an appropriate treatment monitoring is not ensured.To reserve a wide range of possibilities for a life-long ART in HIV-infected patients and in parallel to reduce cost for treatment of both HIV and HCV infection, research focusing on detection, surveillance and transmission of resistance mutations is fundamental to prevent treatment failure on antivirals. In this PhD, we employed the ultra-deep sequencing (UDS) or next-generation sequencing (NGS) technologies to look for minority resistant variants (MiRVs) which are conventionally considered to represent less than 15%-25% of viral population and undetectable by Sanger sequencing. The presence of MiRVs at baseline is possibly responsible for the treatment failure and their presence at failure may limit options for subsequent therapies. In this PhD, we evaluated the prevalence and clinical impact of MiRVs on integrase gene in HIV-infected patients failing an integrase inhibitor containing regimen. We also evaluated the impact of MiRVs in HCV genotype 3 and genotype 4-infected patients failing DAAs. Furthermore, we used the UDS technique to identify and characterize the HCV transmission networks among a key population of men having sex with men either co-infected with HIV or at high risk of HIV acquisition. We also discovered several cases of mixed HCV genotype infections in this population probably for their high risk of multiple HCV exposures. The advantages of UDS in virology research and the applicability of this technique in clinic have been questioned and verified throughout multiple types of projects in this PhD. UDS has not been conclusively established to be more interesting and beneficial than Sanger sequencing in prevention of treatment failure in patients infected by HIV or HCV and in identifying the viral transmission networks at large scale if taking into account the experiment cost and time for data analysis. However, the dynamic development of UDS technologies and the continuing attempts in optimizing analysis procedures display a promising role of UDS. And the applicability of UDS in clinical practice still needs to be elucidated in different kinds of research projects
Engström, Erik. "Direct poly(A) RNA nanopore sequencing on the freshwater duck mussel Anodonta anatina following exposure to copper : A pilot study." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17508.
Full textBusson, Laurent. "Evolution of direct diagnostic techniques in Virology; analytical performances and clinical input." Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/313391.
Full textDoctorat en Sciences médicales (Médecine)
info:eu-repo/semantics/nonPublished
Books on the topic "Direct sequencing"
1937-, Jost J. P., ed. A laboratory guide to genomic sequencing: The direct sequencing of native uncloned DNA. Basel: Birkhäuser, 1987.
Find full textJost and Saluz. Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA. Birkhauser Verlag, 2013.
Find full textJost and Saluz. A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA. Birkhäuser, 2011.
Find full textJost, J. P., and H. P. Saluz. A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA (Biomethods). Birkhauser, 1988.
Find full textCaillaud, Catherine, and Frédéric Sedel. Neuronal Ceroid Lipofuscinoses. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199972135.003.0059.
Full textEllingboe, James. The PCR Technique : DNA Sequencing. Eaton Publishing Company/Biotechniques Books, 1992.
Find full textGraves, Tracey. Neurogenetic disease. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0223.
Full textHarms, Matthew B., and Timothy M. Miller. Amyotrophic Lateral Sclerosis. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199937837.003.0027.
Full textVaheri, Antti, James N. Mills, Christina F. Spiropoulou, and Brian Hjelle. Hantaviruses. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0035.
Full textBook chapters on the topic "Direct sequencing"
Schieltz, David M., and John R. Yates. "Direct Identification of Proteins in Ultracomplex Mixtures." In Protein Sequencing Protocols, 235–45. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1385/1-59259-342-9:235.
Full textRay, Kunal, Arijit Mukhopadhyay, and Mainak Sengupta. "Gene Discovery by Direct Genome Sequencing." In Gene Discovery for Disease Models, 215–33. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470933947.ch11.
Full textMazars, Georges-Raoul, and Charles Theillet. "Direct Sequencing by Thermal Asymmetric PCR." In PCR Protocols, 355–59. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_52.
Full textSmith, Linda K. "HLA Typing by Direct DNA Sequencing." In Methods in Molecular Biology™, 67–86. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-842-9_5.
Full textHong, Tao. "Direct Nucleotide Sequencing for Amplification Product Identification." In Advanced Techniques in Diagnostic Microbiology, 389–402. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-33900-9_19.
Full textDžunková, Mária. "Flow Cytometry and Direct Sequencing of Viruses." In The Human Virome, 3–14. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8682-8_1.
Full textHong, Tao. "Direct Nucleotide Sequencing for Amplification Product Identification." In Advanced Techniques in Diagnostic Microbiology, 383–95. Boston, MA: Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-3970-7_22.
Full textKrautwurst, Sebastian, Ronald Dijkman, Volker Thiel, Andi Krumbholz, and Manja Marz. "Direct RNA Sequencing for Complete Viral Genomes." In Virus Bioinformatics, 35–50. Boca Raton: Chapman and Hall/CRC, 2021. http://dx.doi.org/10.1201/9781003097679-3.
Full textHansen, C., M. Jørgensen, M. Bévort, R. Hummel, M. Løfgreen, N. Pallisgaard, and H. Leffers. "Direct Automated Sequencing of DDRT-PCR Fragments." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 345–51. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_35.
Full textGyllensten, Ulf. "Direct Sequencing of In Vitro Amplified DNA." In PCR Technology, 45–60. London: Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-20235-5_5.
Full textConference papers on the topic "Direct sequencing"
Heath, Samuel A., Franco P. Preparata, and Joel Young. "Sequencing by hybridization using direct and reverse cooperating spectra." In the sixth annual international conference. New York, New York, USA: ACM Press, 2002. http://dx.doi.org/10.1145/565196.565220.
Full textYanagi, Itaru, Rena Akahori, Tomio Iwasaki, Yusuke Goto, Kazuma Matsui, Yoshikazu Nara, Naomi Manri, Mayu Aoki, Takahide Yokoi, and Ken-ichi Takeda. "Fabrication and analysis of SiN nanopores for direct DNA sequencing." In 2015 IEEE International Electron Devices Meeting (IEDM). IEEE, 2015. http://dx.doi.org/10.1109/iedm.2015.7409692.
Full textCox, M., S. Carney, L. Hoyles, S. Roy, R. Williams, J. Breuer, V. Chalker, and M. Moffatt. "Legionella Pneumophila Typing by Direct Sequencing of Clinical and Environmental Samples." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2121.
Full textSchmitt, Michael W., Jesse J. Salk, Edward J. Fox, Justin R. Pritchard, J. Graeme Hodgson, Victor M. Rivera, Pamela S. Becker, Jerald P. Radich, and Lawrence A. Loeb. "Abstract 2395: Direct assessment of sequence heterogeneity in human cancers by Duplex Sequencing." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-2395.
Full textKolodziejski, Noah J., Rajan Gurjar, Karthik Vishwanath, and David Wolf. "Tip-Enhanced Raman Spectroscopy: Focusing in on a Direct Sequencing Method for Oxidized DNA." In Biomedical Optics. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/biomed.2014.bt3a.29.
Full textYanagi, Itaru, Takeshi Oura, Takanobu Haga, Masahiko Ando, Jiro Yamamoto, Toshiyuki Mine, Takeshi Ishida, et al. "A novel side-gated ultrathin-channel nanopore FET (SGNAFET) sensor for direct DNA sequencing." In 2013 IEEE International Electron Devices Meeting (IEDM). IEEE, 2013. http://dx.doi.org/10.1109/iedm.2013.6724629.
Full textSianipar, Nesti Fronika, Reflinur, Muhammad Dzulkifly Ashan, Khoirunnisa Assidqi, Dwityantari Widyaningrum, and Ragapadmi Purnamaningsih. "Direct verification of bogor superior mutant (Typhonium flagelliforme) using RAPD marker techniques and sequencing analysis." In THE 8TH INTERNATIONAL CONFERENCE AND WORKSHOP ON BASIC AND APPLIED SCIENCE (ICOWOBAS) 2021. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0104057.
Full textKarunyakorn, Jirachote, Naruemon Pratanwanich, Monnat Pongpanich, and Pornchai Kaewsapsak. "A Computational Workflow for Estimation of Short RNA Polyadenylation using Direct RNA Nanopore Sequencing with Polyuridylation." In 2022 19th International Joint Conference on Computer Science and Software Engineering (JCSSE). IEEE, 2022. http://dx.doi.org/10.1109/jcsse54890.2022.9836262.
Full textFlusberg, Benjamin, Dale Webster, Kevin Travers, Eric Olivares, Jonas Korlach, and Stephen Turner. "Abstract 1154: Direct detection of DNA methylation and mutagenic damage through single-molecule, real-time (SMRTTM) DNA sequencing." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-1154.
Full textShekhar, Sudip, Rajesh Inti, James Jaussi, Tzu-Chien Hsueh, and Bryan Casper. "A 1.2–5Gb/s 1.4–2pJ/b serial link in 22nm CMOS with a direct data-sequencing blind oversampling CDR." In 2015 Symposium on VLSI Circuits. IEEE, 2015. http://dx.doi.org/10.1109/vlsic.2015.7231319.
Full textReports on the topic "Direct sequencing"
Rhodes, C. K. Human genome sequencing with direct x-ray holographic imaging. Office of Scientific and Technical Information (OSTI), June 1993. http://dx.doi.org/10.2172/6288788.
Full textRhodes, C. K. Human genome sequencing with direct x-ray holographic imaging. Final report. Office of Scientific and Technical Information (OSTI), June 1993. http://dx.doi.org/10.2172/10168190.
Full textGelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, December 2003. http://dx.doi.org/10.32747/2003.7586470.bard.
Full textMehmood, Hamid, Surya Karthik Mukkavilli, Ingmar Weber, Atsushi Koshio, Chinaporn Meechaiya, Thanapon Piman, Kenneth Mubea, Cecilia Tortajada, Kimberly Mahadeo, and Danielle Liao. Strategic Foresight to Applications of Artificial Intelligence to Achieve Water-related Sustainable Development Goals. United Nations University Institute for Water, Environment and Health, April 2020. http://dx.doi.org/10.53328/lotc2968.
Full textFahima, Tzion, and Jorge Dubcovsky. Map-based cloning of the novel stripe rust resistance gene YrG303 and its use to engineer 1B chromosome with multiple beneficial traits. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598147.bard.
Full textTang, Jiqin, Gong Zhang, Jinxiao Xing, Ying Yu, and Tao Han. Network Meta-analysis of Heat-clearing and Detoxifying Oral Liquid of Chinese Medicines in Treatment of Children’s Hand-foot-mouth Disease:a protocol for systematic review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, January 2022. http://dx.doi.org/10.37766/inplasy2022.1.0032.
Full textDubcovsky, Jorge, Tzion Fahima, Ann Blechl, and Phillip San Miguel. Validation of a candidate gene for increased grain protein content in wheat. United States Department of Agriculture, January 2007. http://dx.doi.org/10.32747/2007.7695857.bard.
Full textBercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.
Full textMawassi, Munir, and Valerian V. Dolja. Role of the viral AlkB homologs in RNA repair. United States Department of Agriculture, June 2014. http://dx.doi.org/10.32747/2014.7594396.bard.
Full textLevisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, January 2006. http://dx.doi.org/10.32747/2006.7695853.bard.
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