Relevant bibliographies by topics / Dimers Purification

Academic literature on the topic 'Dimers Purification'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Dimers Purification"

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Tellinghuisen, Timothy L., Rushika Perera, and Richard J. Kuhn. "In Vitro Assembly of Sindbis Virus Core-Like Particles from Cross-Linked Dimers of Truncated and Mutant Capsid Proteins." Journal of Virology 75, no. 6 (March 15, 2001): 2810–17. http://dx.doi.org/10.1128/jvi.75.6.2810-2817.2001.

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ABSTRACT A nucleic acid-bound capsid protein dimer was previously identified using a Sindbis virus in vitro nucleocapsid assembly system and cross-linking reagents. Cross-link mapping, in combination with a model of the nucleocapsid core, suggested that this dimer contained one monomer from each of two adjacent capsomeres. This intercapsomere dimer is believed to be the initial intermediate in the nucleocapsid core assembly mechanism. This paper presents the purification of cross-linked dimers of a truncated capsid protein and the partial purification of cross-linked dimers of a full-length assembly-defective mutant. The assembly of core-like particles from these cross-linked capsid protein dimers is demonstrated. Core-like particles generated from cross-linked full-length mutant CP(19-264)L52D were examined by electron microscopy and appeared to have a morphology similar to that of wild-type in vitro-assembled core-like particles, although a slight size difference was often visible. Truncated cross-linked CP(81-264) dimers generated core-like particles as well. These core-like particles could subsequently be disassembled when reversible cross-linking reagents were used to form the dimers. The ability of the covalent intercapsomere cross-link to rescue capsid proteins with assembly defects or truncations in the amino-terminal region of the capsid protein supports the previous model of assembly and suggests a possible role for the amino-terminal region of the protein.
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Croucher, David R., Mary Iconomou, Jordan F. Hastings, Sean P. Kennedy, Jeremy Z. R. Han, Robert F. Shearer, Jessie McKenna, et al. "Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers." Science Signaling 9, no. 436 (July 12, 2016): ra69. http://dx.doi.org/10.1126/scisignal.aaf0793.

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Singh, S. V., T. Leal, G. A. Ansari, and Y. C. Awasthi. "Purification and characterization of glutathione S-transferases of human kidney." Biochemical Journal 246, no. 1 (August 15, 1987): 179–86. http://dx.doi.org/10.1042/bj2460179.

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Several forms of glutathione S-transferase (GST) are present in human kidney, and the overall isoenzyme pattern of kidney differs significantly from those of other human tissues. All the three major classes of GST isoenzymes (α, mu and pi) are present in significant amounts in kidney, indicating that GST1, GST2 and GST3 gene loci are expressed in this tissue. More than one form of GST is present in each of these classes of enzymes, and individual variations are observed for these classes. The structural, immunological and functional properties of GST isoenzymes of three classes differ significantly from each other, whereas the isoenzymes belonging to the same class have similar properties. All the cationic GST isoenzymes of human kidney except for GST 9.1 are heterodimers of 26,500-Mr and 24,500-Mr subunits. GST 9.1 is a dimer of 24,500-Mr subunits. All the cationic isoenzymes of kidney GST cross-react with antibodies raised against a mixture of GST α, beta, gamma, delta and epsilon isoenzymes of liver. GST 6.6 and GST 5.5 of kidney are dimers of 26,500-Mr subunits and are immunologically similar to GST psi of liver. Unlike other human tissues, kidney has at least two isoenzymes (pI 4.7 and 4.9) associated with the GST3 locus. Both these isoenzymes are dimers of 22,500-Mr subunits and are immunologically similar to GST pi of placenta. Some of the isoenzymes of kidney do not correspond to known GST isoenzymes from other human tissues and may be specific to this tissue.
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Cunha Lima, Suzana T., and Edson D. Rodrigues. "The oligomeric state of thyroid receptor regulates hormone binding kinetics." Journal of Endocrinology 210, no. 1 (April 20, 2011): 125–34. http://dx.doi.org/10.1530/joe-11-0019.

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We previously reported that mutations in the thyroid hormone receptor (TR) surface that mediates dimer and heterodimer formation do not alter affinity for cognate hormone (triiodothyronine (T3)) yet dramatically enhance T3 association and dissociation rates. This study aimed to show that TR oligomeric state influences binding and dissociation kinetics. We performed binding assays using marked hormone (125I-T3) and TRs expressed and purified by different methods. We find that T3 associates with TRs with biphasic kinetics in solution; a rapid step (half-life ±0.1 h) followed by a slower second step (half-life ±5 h) and that purification of monomers suggests that biphasic kinetics are due to the presence of monomers and dimers in our preparations. In support of this idea, incubation of TR ligand binding domain monomers with corepressor peptide induces dimer formation and decreases association rates and T3 binds to, and dissociates from, a TRβ mutant that only forms dimers (TRβD355R) with slow single-phase kinetics. In addition, heterodimer formation with retinoid X receptors also influences ligand binding kinetics. Together, these results suggest that the dimer/heterodimer surface is allosterically coupled to the hormone binding pocket and that different interactions at this surface exert different effects on ligand binding that may be relevant for TR actions in the cell.
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Guo, Xiangxue, Robert W. Harrison, and Phang C. Tai. "Nucleotide-Dependent Dimerization of the C-Terminal Domain of the ABC Transporter CvaB in Colicin V Secretion." Journal of Bacteriology 188, no. 7 (April 1, 2006): 2383–91. http://dx.doi.org/10.1128/jb.188.7.2383-2391.2006.

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ABSTRACT The cytoplasmic membrane proteins CvaB and CvaA and the outer membrane protein TolC constitute the bacteriocin colicin V secretion system in Escherichia coli. CvaB functions as an ATP-binding cassette transporter, and its C-terminal domain (CTD) contains typical motifs for the nucleotide-binding and Walker A and B sites and the ABC signature motif. To study the role of the CvaB CTD in the secretion of colicin V, a truncated construct of this domain was made and overexpressed. Different forms of the CvaB CTD were found during purification and identified as monomer, dimer, and oligomer forms by gel filtration and protein cross-linking. Nucleotide binding was shown to be critical for CvaB CTD dimerization. Oligomers could be converted to dimers by nucleotide triphosphate-Mg, and nucleotide release from dimers resulted in transient formation of monomers, followed by oligomerization and aggregation. Site-directed mutagenesis showed that the ABC signature motif was involved in the nucleotide-dependent dimerization. The spatial proximity of the Walker A site and the signature motif was shown by disulfide cross-linking a mixture of the A530C and L630C mutant proteins, while the A530C or L630C mutant protein did not dimerize on its own. Taken together, these results indicate that the CvaB CTD formed a nucleotide-dependent head-to-tail dimer.
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Hannon, Clare, Abimael Cruz-Migoni, Olga Platonova, Robin L. Owen, Joanne E. Nettleship, Ami Miller, Stephen B. Carr, Gemma Harris, Terence H. Rabbitts, and Simon E. V. Phillips. "Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor." Acta Crystallographica Section F Structural Biology Communications 74, no. 3 (February 26, 2018): 143–49. http://dx.doi.org/10.1107/s2053230x18001553.

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Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed inEscherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05 Å. The crystals belonged to space groupP21, with eight polypeptide chains in the asymmetric unit arranged as an unusual octamer composed of four domain-swapped IBD dimers. IBD exists as a mixture of monomers and dimers in concentrated solutions, but the dimers are unlikely to be biologically relevant.
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Spencer, J. B., and P. M. Jordan. "Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum." Biochemical Journal 288, no. 3 (December 15, 1992): 839–46. http://dx.doi.org/10.1042/bj2880839.

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6-Methylsalicylic acid synthase has been isolated in homogeneous form from Penicillium patulum grown in liquid culture from a spore inoculum. The enzyme is highly susceptible to proteolytic degradation in vivo and in vitro, but may be stabilized during purification by incorporating proteinase inhibitors in the buffers. The enzyme exists as a homotetramer of M(r) 750,000, with a subunit M(r) of 180,000. 6-Methylsalicyclic acid synthase also accepts acetoacetyl-CoA as an alternative starter molecule to acetyl-CoA. The enzyme also catalyses the formation of small amounts of triacetic acid lactone as an oligatory by-product of the reaction. In the absence of NADPH, triacetic acid lactone is the exclusive enzymic product, being formed at 10% of the rate of 6-methylsalicylic acid. The enzyme is inactivated by 1,3-dibromopropan-2-one, leading to the formation of cross-linked dimers similar to that observed with type I fatty acid synthases. Acetyl-CoA protects the enzyme against the inactivation and inhibits dimer formation. An adaptation of the purification method for 6-methylsalicylic acid synthase may be used for the isolation of fatty acid sythase from Penicillium patulum.
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Gómez, J. "Purification and properties of hydrophilic dimers of acetylcholinesterase from mouse erythrocytes." International Journal of Biochemistry & Cell Biology 35, no. 7 (July 2003): 1109–18. http://dx.doi.org/10.1016/s1357-2725(03)00007-4.

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Brennan, Bradley J., Jaro Arero, Paul A. Liddell, Thomas A. Moore, Ana L. Moore, and Devens Gust. "Selective oxidative synthesis of meso-beta fused porphyrin dimers." Journal of Porphyrins and Phthalocyanines 17, no. 04 (April 2013): 247–51. http://dx.doi.org/10.1142/s1088424613500363.

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An efficient route to meso-β doubly connected fused porphyrin dimers was developed. Synthesis of the dimers incorporated two successive C–C bond-forming steps selectively coupling unsubstituted meso- and β-positions. Using Cu(BF4)2 as an oxidant in nitromethane solvent, the radical coupling of Cu(II) -porphyrins occurred in high yield and without side-products, allowing chromatography-free purification. Efficient demetalation of the product yielded free-base derivatives and the possibility to incorporate other metals into the macrocycles. The absorption and electrochemical properties vary with the inserted metal, showing broad UV-visible-NIR absorption and multiple one-electron oxidations/reductions in a relatively narrow electrochemical window.
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FAMULSKI, Konrad S., Michel LIUZZI, Saber BASHIR, Razmik MIRZAYANS, and Malcolm C. PATERSON. "Purification and characterization of a novel human acidic nuclease/intra-cyclobutyl-pyrimidine-dimer-DNA phosphodiesterase." Biochemical Journal 345, no. 3 (January 25, 2000): 583–93. http://dx.doi.org/10.1042/bj3450583.

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A novel N-glycosylated, mannose-rich protein has been purified approx. 4000-fold from human liver in a seven-step procedure including ion-exchange chromatography and fractionation on concanavalin A-Sepharose, Sephadex G-75 and oligo(dT)-cellulose matrices. The molecular mass of the protein is 46 kDa when measured by gel filtration (i.e. under non-denaturing conditions) and 60 kDa by SDS/PAGE (i.e. under denaturing conditions). The protein possesses two DNA backbone-incising activities, namely, the random introduction of single-strand breaks in native DNA and the rupture of the phosphodiester linkage internal to cyclobutyl pyrimidine dimers, the major class of DNA lesions induced by solar UV rays. Both activities are optimal at pH 5.0 in vitro, although the non-specific nuclease displays appreciable activity at neutral pH, depending on the buffer composition. The protein has been named acidic nuclease/intra-cyclobutyl-pyrimidine-dimer-DNA phosphodiesterase (AN/IDP). As a nuclease, the protein ‘prefers#x2019; a linear DNA structure over a covalently closed circular molecule and is more proficient at digesting single-stranded than double-stranded DNA. The polynucleotide cleavage products of the nuclease contain 5ʹ-OH and 3ʹ-PO4 termini, which are refractory to direct rejoining by DNA ligases. Depending on the substrate, the nuclease activity exhibits a temperature optimum of 50 °C or greater, and is neither stimulated by Mg2+ or Ca2+ nor inhibited by Zn2+. AN/IDP is present in human liver and in cultured human cells of both fibroblastic and lymphocytic origins. Intracellularly, the protein can be readily detected in both the cytosolic and nuclear fractions, although much more (approx. 3-fold) is found in the latter fraction. We propose that this bifunctional enzyme may be involved in both apoptotic DNA digestion and metabolism of cyclobutyl pyrimidine dimers in UV-irradiated human cells.
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Dissertations / Theses on the topic "Dimers Purification"

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PELEIAS, JUNIOR FERNANDO dos S. "Desenvolvimento da metodologia de síntese e purificação dos dímeros L-lactídeo e glicolídeo para produção do poli (ácido lático-co-ácido glicólico) para utilização na produção de fontes radioativas." reponame:Repositório Institucional do IPEN, 2017. http://repositorio.ipen.br:8080/xmlui/handle/123456789/28054.

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Submitted by Pedro Silva Filho (pfsilva@ipen.br) on 2017-11-23T12:28:11Z No. of bitstreams: 0
Made available in DSpace on 2017-11-23T12:28:12Z (GMT). No. of bitstreams: 0
A Organização Mundial da Saúde (OMS) relata o câncer como uma das principais causas de morte no mundo. O câncer de próstata é o segundo tipo de câncer mais prevalente em homens, com cerca de 1,1 milhão de casos diagnosticados em 2012. Braquiterapia com iodo-125 é uma método de radioterapia que consiste na introdução de sementes com material radioativo no interior do órgão a ser tratado. As sementes de iodo-125 podem ser inseridas soltas ou em cordas poliméricas bioabsorvíveis, mais comumente o poli(ácido lático-co-ácido glicólico) (PLGA). A função do polímero é reduzir a possibilidade de migração das sementes, o que poderia ser prejudicial para órgãos e tecidos saudáveis. De modo a reduzir os custos do tratamento, a síntese dos dímeros L-lactídeo e glicolídeo, para posterior utilização para preparação do PLGA, por meio da polimerização por abertura de anel, é proposta neste trabalho. Adicionalmente, propõe-se a utilização do amino-alcóxido tris(fenolato) de zircônio (IV) como alternativa ao usual octanoato de estanho (SnOct2), uma vez que a toxicidade do estanho permanece como obstáculo na produção do PLGA para aplicações biomédicas. Embora o iniciador de zircônio seja mais lento do que o SnOct2, massas molares relativamente elevadas foram obtidas quando razões monômero/iniciador (M/I) de 1000/1 (24 h), e 5000/1 (48 h) foram utilizadas. Considerando que as unidades glicolila (GA) são mais reativas do que as unidades lactila (LA), tempos longos de reação são necessários para atingir uma razão LA/GA próxima do objetivo do trabalho (85/15). O grau de racemização também depende do iniciador utilizado. As reações de polimerização realizadas com o iniciador de zircônio mostraram um maior grau de racemização, quando comparadas com aquelas realizadas com o SnOct2. Também foi observado um ligeiro aumento na racemização com o tempo. Considerando os resultados obtidos na síntese e purificação dos dímeros, e na síntese do PLGA em condições semelhantes às industriais, foi possível preparar o polímero de alta massa molar com um custo dezenas de vezes inferior ao custo do PLGA no mercado internacional. Os efeitos da radiação gama no PLGA também foram estudados. Doses normalmente aplicadas para esterilizar materiais para aplicações biomédicas foram empregadas: 10, 18, 25 e 50 kGy. A massa molar de todas as amostras irradiadas diminuiu de uma forma proporcional à dose até 56% de perda para 10 kGy e 72% para 50 kGy porém, são menos pronunciadas para doses mais elevadas. Alterações nas propriedades térmicas, tais como temperatura de fusão, temperatura de transição vítrea e a entalpia de cristalização e fusão foram também observadas após a irradiação.
Tese (Doutorado em Tecnologia Nuclear)
IPEN/T
Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP
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Wright-Weber, Brianne. "Physical, kinetic, and immunological studies of monomeric (Periplaneta americana) and dimeric (Isostychopus badonotus) arginine kinases." [Tampa, Fla.] : University of South Florida, 2007. http://purl.fcla.edu/usf/dc/et/SFE0002057.

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Held, Brenda Christine. "Examining the evolution of phosphagen kinases : a study of a dimeric arginine kinase from sea urchin (Strongylocentrotus purpuratus) eggs." [Tampa, Fla] : University of South Florida, 2007. http://purl.fcla.edu/usf/dc/et/SFE0001951.

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Boutigny, Anne-Laure. "Etude de l'effet de composés du grain de blé dur sur la régulation de la voie de biosynthèse des trichothécènes B : purification de composés inhibiteurs, analyse des mécanismes impliqués." Bordeaux 1, 2007. http://www.theses.fr/2007BOR13478.

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La résistance à l'accumulation de mycotoxines de Fusarium chez certaines variétés de blé dur résulte de mécanismes naturels, en particulier, de composés endogènes du grain pourraient limiter l'accumulation des trichothécènes par une inhibition de leur biosynthèse. Dans ce contexte, l'objectif principal de cette étude est de rechercher des composés du blé dur inhibiteurs de la toxinognése, d'éudier leurs effets et les mécanismes de régulaion mis en jeu. Des conditions expérimentales permettant d'étudier in vitro l'effet "anti mycotoxines" de composés à tester ont été mises au point. Ceci a permis de montrer l'efficacité de certains acides phénoliques présents dans les sons de blé pour inhiber la production de trichothécènes. Considérés de façon isolée ou en mélange, les acides phénoloqies dérivés de l'acide cinnamique, à des concentrations proches des valeurs physiologiques, limitent l'accumulation de toxines par Fusarium culmorum. Dans un extrait naturel d'acides phénoliques de blé, la présence d'oligonères d'acide férulique augmentent fortement l'efficacité inhibitrice. L'inhibition de la production de toxines par ces acides phénoliques s'explique par la forte régulation au niveau transcriptionnel de l'expression de certainsgènes de la voie de biosynthèse des trichothécènes. La recherche dans les sons de blé de composés inhibiteurs par une démarche sans à priori sur leur nature à permis la purification d'un dimère d'acide phénolique avant une activité "anti mycotoxines" près de dix fois supérieure à celle des formes monomériques. Les diméres d'acide phénolique pourraient ainsi constituer des facteurs clés de résistance à l'accumulation de mycotoxines. Plus généralement, les variations de contenu et de disponibilité en acides phénoliques selon les variétés contribueraient aux différences de sensiblité à l'accumulation de mycotoxines chez le blé dur.
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Book chapters on the topic "Dimers Purification"

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Adachi, Hideyuki, Isao Enami, Takahiro Henmi, Nobuo Kamiya, and Jian-Ren Shen. "Purification And Crystallization Of Photosystem Ii Dimer Complex From A Red Alga Cyanidium Caldarium." In Photosynthesis. Energy from the Sun, 353–56. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6709-9_80.

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Conference papers on the topic "Dimers Purification"

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Holvoet, P., J. M. Stassen, and D. Collen. "THROMBUS IMAGING WITH MURINE MONOCLONAL ANTIBODIES AGAINST FIBRIN FRAGMENT D-DIMER IN A RABBIT JUGULAR VEIN THROMBOSIS MODEL." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642891.

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Three murine monoclonal antibodies (MA-6C1, MA-8D3 and MA-15C5) reacting with fragment D-dimer from crosslinked fibrin but not with monomeric fragment D were obtained by immunization of Balb/c mice with the highly purified fragment, fusion of spleen cells with a myeloma cell line, production of. ascites fluid in mice and purification of the antibodies on Affigel Blue. Fab fragments were isolated from papain digests. The IgG and Fab fragments were labeled with 125I, 131I or 123I using lactoper-oxidase. The disposition rates (t1/2) and thrombus to blood ratios, measured in groups of 3 rabbits with a non-occlusive jugular vein thrombus composed of whole human plasma were :These results indicate that, after 1 to 3 half-lives of the IgG or Fab fragments, using combinations of 2 or 3 monoclonal antibodies, thrombus to blood ratios of isotope of 5 to 7 are obtained. Such signal/noise ratios are sufficient for in vivo detection by exteijil gamma scintigraphy. This was preliminarily confirmed using 123I-labeled Fab fragments of the three antibodies in rabbits.
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