Relevant bibliographies by topics / Dimensional gel electrophoresis

Academic literature on the topic 'Dimensional gel electrophoresis'

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Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Dimensional gel electrophoresis"

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Imajoh-Ohmi, Shinobu. "O'Farrell's two-dimensional gel electrophoresis." SEIBUTSU BUTSURI KAGAKU 34, no. 6 (1990): 307–15. http://dx.doi.org/10.2198/sbk.34.307.

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Lehr, Stefan, and Reiner Westermeier. "2-dimensional gel electrophoresis reloaded." Archives of Physiology and Biochemistry 119, no. 3 (July 2013): 93. http://dx.doi.org/10.3109/13813455.2013.812122.

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Viswanathan, Surya, Mustafa Ünlü, and Jonathan S. Minden. "Two-dimensional difference gel electrophoresis." Nature Protocols 1, no. 3 (August 2006): 1351–58. http://dx.doi.org/10.1038/nprot.2006.234.

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Serwer, Philip. "Two-dimensional agarose gel electrophoresis without gel manipulation." Analytical Biochemistry 144, no. 1 (January 1985): 172–78. http://dx.doi.org/10.1016/0003-2697(85)90100-9.

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Garfin, David E. "Two-dimensional gel electrophoresis: an overview." TrAC Trends in Analytical Chemistry 22, no. 5 (May 2003): 263–72. http://dx.doi.org/10.1016/s0165-9936(03)00506-5.

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Gallagher, Sean R., and John A. Smith. "One-Dimensional Gel Electrophoresis of Proteins." Current Protocols in Immunology 00, no. 1 (December 1991): 8.4.1. http://dx.doi.org/10.1002/0471142735.im0804s00.

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Danos, Olivier, and Fedor Svinartchouk. "Dialysis-assisted two-dimensional gel electrophoresis." ELECTROPHORESIS 27, no. 17 (September 2006): 3475–79. http://dx.doi.org/10.1002/elps.200500036.

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Dunn, Michael J. "Two-dimensional gel electrophoresis of proteins." Journal of Chromatography B: Biomedical Sciences and Applications 418 (July 1987): 145–85. http://dx.doi.org/10.1016/0378-4347(87)80008-7.

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Liu, Yi-Ming, and Jonathan V. Sweedler. "Two-Dimensional Separations: Capillary Electrophoresis Coupled to Channel Gel Electrophoresis." Analytical Chemistry 68, no. 22 (January 1996): 3928–33. http://dx.doi.org/10.1021/ac960549+.

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Shaw, Margaret M., and Beat M. Riederer. "Sample preparation for two-dimensional gel electrophoresis." PROTEOMICS 3, no. 8 (August 2003): 1408–17. http://dx.doi.org/10.1002/pmic.200300471.

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Dissertations / Theses on the topic "Dimensional gel electrophoresis"

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Rye, Morten Beck. "Image segmentation and multivariate analysis in two-dimensional gel electrophoresis." Doctoral thesis, Norwegian University of Science and Technology, Department of Chemistry, 2007. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-1744.

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The topic of this thesis is data-analysis on images from two-dimensional electrophoretic gels. Because of the complexity of these images, there are numerous steps and approaches to such an analysis, and no “golden standard” has yet been established on how to produce the desired output. In this thesis focus is put on two essential fields concerning 2D-gel analysis; registration of images by segregation and protein spot identification, and data-analysis on the output of such a registration by multivariate methods. Image segmentation is mainly concerned with the task of identifying individual protein spots in a gel-image. This has generally been the natural starting point of all methods and procedures developed since the introduction of 2D-gels in the mid-seventies, simply because this best reproduces the results created by a human analyst, who manually identify protein-spot entities. The amount of data produced in a 2D-gel experiment can be quite large, especially in multiple gels where the human analyst is dependent on additional statistical data-analytical tools to produce results. Because of the correlated nature of most gel-data, analysis by multivariate methods is natural choice, and are therefore adopted in this thesis. The goal of this thesis is to introduce the above mentioned procedures at different stages in the analysis pipeline where they are not yet fully exploited, rather than to improve already existing algorithms. In this way new insight and ideas on how to handle data from 2D-gel experiments are achieved. The thesis starts with a review of segmentation methodology, and introduces a selected procedure used to identify protein spots throughout. Output from the segmentation is then used to create a multivariate spot-filtering model, which aims to separate protein spots from noise and artefacts often creating problems in 2D-gel analysis. Lately the use of common spot boundaries in multiple gels have been the method of choice when gels are analysed. How such boundaries should be defined is an important subject of discussion, and thus a new method for defining common boundaries based on the individual segmentation of each gel is introduced. Segmentation may be a natural starting point when gels are analysed, but it is not necessarily the most correct. Often the introduction of fixed spot entities introduces restrictions to the data which cause problems at later stages in the analysis. Analysing pixels from multiple gels directly has no such restrictions, and it is shown in this thesis that the output of such an analysis based on multivariate methods can produce very useful results. It can also give insight to the data problematic to achieve with the spot boundary approach. At last in the thesis an improved pixel-based approach is introduced, where a less restricted segmentation is used to reduce and concentrate the amount of data analysed, improving the final output.

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Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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Xu, Aoshuang. "Development in electrophoresis instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis /." [Ames, Iowa : Iowa State University], 2008.

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Yao, Mingyi. "Proteomic studies of Pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis /." Online version of thesis, 2005. https://ritdml.rit.edu/dspace/handle/1850/1118.

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Fowlkes, Kelly. "A proteomic study of Pseudomonas putida by two-dimensional gel electrophoresis : establishing quantitative standards for intra-laboratory results /." Online version of thesis, 2007. http://hdl.handle.net/1850/5040.

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Pandey, Archana. "Proteome analysis of Pseudomonas putida KT2440 using 2D gel electrophoresis and LC/ESI-Q-TOF mass spectrometry /." Online version of thesis, 2007. https://ritdml.rit.edu/dspace/handle/1850/3848.

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Rajagopal, Meena Uma. "METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS." UKnowledge, 2006. http://uknowledge.uky.edu/gradschool_diss/292.

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The development of a highly sensitive ruthenium-based fluorescent staining solution isdescribed in this dissertation. The in-house synthesized ruthenium complex (RuMS)containing both sulfonated and non-sulfonated ligand has detection limit of 1 ng ofprotein that is better than colloidal coomassie, silver and ruthenium complex containingall sulfonated ligands (RuBPS). RuMS stain has 100-fold dynamic range and does notinterfere with subsequent mass spectral identification of proteins. The capability of inhousesynthesis of the staining solution makes it a viable cost-effective alternative to theexpensive commercially available fluorescent stain, Sypro Ruby. The low detection limit,broad linear dynamic range and compatibility with mass spectrometry, make thedevelopment of this stain a worthwhile pursuit. The staining solution was utilized insubsequent applications of two-dimensional gel electrophoresis (2-DE) technology.Proteomics methodology utilizing 2-DE and mass spectrometry was applied toinvestigate the effect of malathion on the proteome of human neuroblastoma cells.Results indicated that out of 122 proteins that were identified from the neuroblastomaproteome, sixteen proteins were down-regulated while five proteins were significantlyup-regulated after treatment with malathion. Significant down-regulation of calciummodulators like calmodulin and calgizarrin and other key chaperones makes themalathion-treated cells highly prone to oxidative stress. With increased awareness inpesticide related adverse effects, identification of altered proteins in malathion-treatedhuman neuroblastoma cells is a critical finding.Proteomics is a major area of research in the identification of biomarkers for diseases. Anovel immunoprecipitation method developed in this work allowed for successfulisolation and identification of albumin-interactome in cerebrospinal fluid (CSF) that isusually under-represented in standard CSF analysis using 2-DE. A key finding is thedifferential expression of various isoforms of proteins in CSF albumin-interactome fromAlzheimer's disease (AD) subjects. The data implicate the acidic isoform ofprostaglandin D2 synthase (PGDS2) as a potential biomarker for AD. An understandingof the differential expression of these protein isoforms in AD will provide insight into theetiology of the disease and this can have far-reaching implication on drug developmentleading to the cure or even preventation of the disease.
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Ethell, Douglas Wayne. "Analysis of developing chick Gallus domesticus spinal cord proteins using two dimensional gel electrophoresis." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29834.

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Several recent experiments on developing chick spinal cord have established a time window when the developing spinal cord changes from a permissive to a restrictive environment for regeneration. This time window occurs during embryonic days 13-14 (E13-E14) of chick development. Recent experiments in adult rat, have found two proteins that actively inhibit axonal regeneration. This study has sought possible inhibitory proteins, in chicks, correlating to this temporal change. Proteins continuously present after this change (E14-E20) but not before (E11) were identified. Two-dimensional gel electrophoresis was used for separatation of the proteins. Seven protein spots of interest demonstrated this correlative late-expressing neural protein (LNP) profile. Although the functions of these proteins could not be ascertained in this study, further investigation is warranted.
Science, Faculty of
Zoology, Department of
Graduate
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Schwartz, Anne. "Characterization of normal and androgen resistant-genital skin fibroblasts using high-resolution two-dimensional gel electrophoresis." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63378.

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Anduri, Sridevi. "Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis a thesis presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=2000377671&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1277817194&clientId=28564.

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Books on the topic "Dimensional gel electrophoresis"

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Two-dimensional electrophoresis protocols. New York: Humana, 2009.

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Dunbar, Bonnie S. Two-dimensional electrophoresis, and immunological techniques. New York: Plenum Press, 1987.

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Two-dimensional electrophoresis, and immunological techniques. New York: Plenum Press, 1987.

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Rabilloud, Thierry, ed. Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57105-3.

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International, Two-Dimensional Electrophoresis Conference (1988 Vienna Austria). Two-dimensional electrophoresis: Proceedings of the International Two-Dimensional Electrophoresis Conference, Vienna, November 1988. Weinheim, F.R.G: VCH, 1989.

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Anderson, Leigh. Two-dimensional electrophoresis: Operation of the ISO-DALT system. Washington, D.C: Large Scale Biology Press, 1988.

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Rust, Nigel Anthony. Analysis of the human MCH class II antigens by two dimensional gel electrophoresis. Oxford: Oxford Polytechnic, 1986.

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Uitterlinden, André G. Two-dimensional DNA typing: A parallel approach to genome analysis. New York: E. Horwood, 1994.

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Bollag, Daniel M. Protein methods. New York: Wiley-Liss, 1991.

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D, Rozycki Michael, and Edelstein Stuart J, eds. Protein methods. 2nd ed. New York: Wiley-Liss, 1996.

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Book chapters on the topic "Dimensional gel electrophoresis"

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Turiault, Marc, Caroline Cohen, Guy Griebel, David E. Nichols, Britta Hahn, Gary Remington, Ronald F. Mucha, et al. "Two-Dimensional Gel Electrophoresis." In Encyclopedia of Psychopharmacology, 1351. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1547.

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Minden, Jonathan S. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 287–304. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-821-4_24.

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Robinson, Aisling A., Ciara A. McManus, and Michael J. Dunn. "Two-Dimensional Polyacrylamide Gel Electrophoresis." In Sample Preparation in Biological Mass Spectrometry, 217–42. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0828-0_13.

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Blundon, Malachi, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 229–47. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8793-1_20.

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Bergh, Gert Van den. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 439–54. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-281-6_29.

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Kłodzińska, Ewa, and Bogusław Buszewski. "Two-dimensional Gel Electrophoresis (2DE)." In Springer Series in Chemical Physics, 133–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-35043-6_8.

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Dunbar, Bonnie S. "Protein Detection in Polyacrylamide Gel Electrophoresis." In Two-Dimensional Electrophoresis and Immunological Techniques, 67–76. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1957-3_4.

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Römling, Ute, Karen Schmidt, and Burkhard Tümmler. "One-dimensional Pulsed-field Gel Electrophoresis." In Bacterial Genomes, 312–25. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_25.

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Römling, Ute, Karen Schmidt, and Burkhard Tümmler. "Two-dimensional Pulsed-field Gel Electrophoresis." In Bacterial Genomes, 326–36. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_26.

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Ackermann, Doreen, and Simone König. "Comparative Two-Dimensional Fluorescence Gel Electrophoresis." In Methods in Molecular Biology, 69–78. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7268-5_6.

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Conference papers on the topic "Dimensional gel electrophoresis"

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"MATCHING TWO-DIMENSIONAL GEL ELECTROPHORESIS’ SPOTS." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2012. http://dx.doi.org/10.5220/0003702401110117.

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Xin, Hua-Mei, Yuemin Zhu, Lin Xue, Robert Goutte, and Long-Fei Wu. "New Registration Method for Two-Dimensional Gel Electrophoresis Images." In 2009 2nd International Conference on Biomedical Engineering and Informatics. IEEE, 2009. http://dx.doi.org/10.1109/bmei.2009.5305071.

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Xin, Huamei, and Fengping Zhao. "Effective denoising methods for two-dimensional gel electrophoresis images." In 2011 4th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2011. http://dx.doi.org/10.1109/bmei.2011.6098614.

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Savelonas, Michalis, Dimitris Maroulis, and Eleftheria Mylona. "Segmentation of two-dimensional gel electrophoresis images containing overlapping spots." In 2009 9th International Conference on Information Technology and Applications in Biomedicine (ITAB 2009). IEEE, 2009. http://dx.doi.org/10.1109/itab.2009.5394327.

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Ahmad, Norhaiza, J. Zhang, P. J. Brown, D. C. James, J. R. Birch, A. J. Racher, C. M. Smales, Kamel Ariffin Mohd Atan, and Isthrinayagy S. Krishnarajah. "Proteomic Profiling Of Two-Dimensional Gel Electrophoresis Protein Expression Data." In INTERNATIONAL CONFERENCE ON MATHEMATICAL BIOLOGY 2007: ICMB07. AIP, 2008. http://dx.doi.org/10.1063/1.2883868.

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Tung-Shou Chen, Tian-Shing Wang, Wao-Chuan Hsiao, and Chun-Wei Tsai. "An Integrated Web-Based System for Two-Dimensional Electrophoresis Gel Images." In 8th International Conference on Advanced Communication Technology. IEEE, 2006. http://dx.doi.org/10.1109/icact.2006.206002.

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Ahmed, Aya Saleh, Wessam H. El-Behaidy, and Aliaa A. A. Youssif. "Automatic Enhancement of Two-Dimensional Gel electrophoresis images using Denoising Autoencoder." In 2019 14th International Conference on Computer Engineering and Systems (ICCES). IEEE, 2019. http://dx.doi.org/10.1109/icces48960.2019.9068175.

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Li, Ling, Yuanyuan Song, Yifeng Wang, Baoxin Zheng, and Tieling Liu. "Improvement of two-dimensional gel electrophoresis conditions for rin mutant tomato fruit." In 2015 3rd International Conference on Advances in Energy and Environmental Science. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/icaees-15.2015.232.

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Deng, Zhaohong, Shitong Wang, Qun Gao, and Yonghui Pan. "Fuzzy inference mechanism based automatic elastic registration of two-dimensional gel electrophoresis images." In 2011 Eighth International Conference on Fuzzy Systems and Knowledge Discovery (FSKD 2011). IEEE, 2011. http://dx.doi.org/10.1109/fskd.2011.6019724.

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Cary, Ariel, Davor Pavisic, and Reynaldo Vargas. "Feedforward Neural Networks for the Classification of Two-dimensional Polyacrylamide Gel Electrophoresis Images." In 5. Congresso Brasileiro de Redes Neurais. CNRN, 2016. http://dx.doi.org/10.21528/cbrn2001-007.

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Reports on the topic "Dimensional gel electrophoresis"

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Dunn, Bruce E., Martin J. Blaser, and Edward L. Snyder. Two-Dimensional Gel Electrophoresis and Immunoblotting of Campylobacter Outer Membrane Proteins. Fort Belvoir, VA: Defense Technical Information Center, April 1987. http://dx.doi.org/10.21236/ada265461.

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Xu, Aoshuang. Development in electrophoresis: instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis. Office of Scientific and Technical Information (OSTI), January 2008. http://dx.doi.org/10.2172/1342558.

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