Relevant bibliographies by topics / Digital polymerase chain reaction

Academic literature on the topic 'Digital polymerase chain reaction'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Digital polymerase chain reaction"

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Ahrberg, Christian D., Andreas Manz, and Bong Geun Chung. "Polymerase chain reaction in microfluidic devices." Lab on a Chip 16, no. 20 (2016): 3866–84. http://dx.doi.org/10.1039/c6lc00984k.

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Fan, H. Christina, and Stephen R. Quake. "Detection of Aneuploidy with Digital Polymerase Chain Reaction." Analytical Chemistry 79, no. 19 (October 2007): 7576–79. http://dx.doi.org/10.1021/ac0709394.

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Chang, Yi-Hsien, Gwo-Bin Lee, Fu-Chun Huang, Yi-Yu Chen, and Jr-Lung Lin. "Integrated polymerase chain reaction chips utilizing digital microfluidics." Biomedical Microdevices 8, no. 3 (May 20, 2006): 215–25. http://dx.doi.org/10.1007/s10544-006-8171-y.

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Roden, Anja C., Julie A. Vrana, Justin W. Koepplin, Angela E. Hudson, Andrew P. Norgan, Garrett Jenkinson, Satoko Yamaoka, et al. "Comparison of In Situ Hybridization, Immunohistochemistry, and Reverse Transcription–Droplet Digital Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Testing in Tissue." Archives of Pathology & Laboratory Medicine 145, no. 7 (March 15, 2021): 785–96. http://dx.doi.org/10.5858/arpa.2021-0008-sa.

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Context.— Small case series have evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in formalin-fixed, paraffin-embedded tissue using reverse transcription–polymerase chain reaction, immunohistochemistry (IHC), and/or RNA in situ hybridization (RNAish). Objective.— To compare droplet digital polymerase chain reaction, IHC, and RNAish to detect SARS-CoV-2 in formalin-fixed, paraffin-embedded tissue in a large series of lung specimens from coronavirus disease 2019 (COVID-19) patients. Design.— Droplet digital polymerase chain reaction and RNAish used commercially available probes; IHC used clone 1A9. Twenty-six autopsies of COVID-19 patients with formalin-fixed, paraffin-embedded tissue blocks of 62 lung specimens, 22 heart specimens, 2 brain specimens, and 1 liver, and 1 umbilical cord were included. Control cases included 9 autopsy lungs from patients with other infections/inflammation and virus-infected tissue or cell lines. Results.— Droplet digital polymerase chain reaction had the highest sensitivity for SARS-CoV-2 (96%) when compared with IHC (31%) and RNAish (36%). All 3 tests had a specificity of 100%. Agreement between droplet digital polymerase chain reaction and IHC or RNAish was fair (κ = 0.23 and κ = 0.35, respectively). Agreement between IHC and in situ hybridization was substantial (κ = 0.75). Interobserver reliability was almost perfect for IHC (κ = 0.91) and fair to moderate for RNAish (κ = 0.38–0.59). Lung tissues from patients who died earlier after onset of symptoms revealed higher copy numbers by droplet digital polymerase chain reaction (P = .03, Pearson correlation = −0.65) and were more likely to be positive by RNAish (P = .02) than lungs from patients who died later. We identified SARS-CoV-2 in hyaline membranes, in pneumocytes, and rarely in respiratory epithelium. Droplet digital polymerase chain reaction showed low copy numbers in 7 autopsy hearts from ProteoGenex Inc. All other extrapulmonary tissues were negative. Conclusions.— Droplet digital polymerase chain reaction was the most sensitive and highly specific test to identify SARS-CoV-2 in lung specimens from COVID-19 patients.
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Sun, Chen, Leqian Liu, Harish N. Vasudevan, Kai-Chun Chang, and Adam R. Abate. "Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction." Analytical Chemistry 93, no. 29 (July 12, 2021): 9974–79. http://dx.doi.org/10.1021/acs.analchem.1c00877.

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White, A. K., K. A. Heyries, C. Doolin, M. VanInsberghe, and C. L. Hansen. "High-Throughput Microfluidic Single-Cell Digital Polymerase Chain Reaction." Analytical Chemistry 85, no. 15 (July 24, 2013): 7182–90. http://dx.doi.org/10.1021/ac400896j.

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Vynck, Matthijs, Wim Trypsteen, Olivier Thas, Linos Vandekerckhove, and Ward De Spiegelaere. "The Future of Digital Polymerase Chain Reaction in Virology." Molecular Diagnosis & Therapy 20, no. 5 (June 28, 2016): 437–47. http://dx.doi.org/10.1007/s40291-016-0224-1.

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Sundberg, Scott O., Carl T. Wittwer, Chao Gao, and Bruce K. Gale. "Spinning Disk Platform for Microfluidic Digital Polymerase Chain Reaction." Analytical Chemistry 82, no. 4 (February 15, 2010): 1546–50. http://dx.doi.org/10.1021/ac902398c.

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Byrnes, Samantha A., Tim C. Chang, Toan Huynh, Anna Astashkina, Bernhard H. Weigl, and Kevin P. Nichols. "Simple Polydisperse Droplet Emulsion Polymerase Chain Reaction with Statistical Volumetric Correction Compared with Microfluidic Droplet Digital Polymerase Chain Reaction." Analytical Chemistry 90, no. 15 (July 9, 2018): 9374–80. http://dx.doi.org/10.1021/acs.analchem.8b01988.

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Gaňová, Martina, Haoqing Zhang, Hanliang Zhu, Marie Korabečná, and Pavel Neužil. "Multiplexed digital polymerase chain reaction as a powerful diagnostic tool." Biosensors and Bioelectronics 181 (June 2021): 113155. http://dx.doi.org/10.1016/j.bios.2021.113155.

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Dissertations / Theses on the topic "Digital polymerase chain reaction"

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Al-hashimi, Sora. "Kvantifiering med digital droplet polymerase chain reaction av gyrA-genen med och utan mutationen S83L." Thesis, Örebro universitet, Institutionen för hälsovetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-92989.

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Den vanligaste cancerformen hos män är prostatacancer, med 10 000 nya dödsfall årligen. Vid prostatacancerdiagnostik utförs prostata biopsi. För att minska risken för komplikationer i samband med biopsi ges i Sverige en singeldos av antibiotikapreparatet Ciprofloxacin. Andelen bakterier som är resistenta mot ciprofloxacin har ökat. För detektion av genmutationer som orsakar antibiotikaresistens kan droplet digital PCR (ddPCR) användas. Det är en metod som ger en absolut kvantifiering av antalet DNA-sekvenser. Den är baserad på vatten olja-emulsionsdroppsystem.  Syftet med denna studie var att optimera och validera en digital droplet PCR för att detektera och kvantifiera mutationen S83L i gyrA genen från faecesprover, samt att jämföra digital droplet resultat från studieprover med odlingsresultat från resistensbestämning och ration mellan S83L allelen och vildtyps allelen i prover tagna före och efter biopsi. För att optimera och validera metoden användes prover tagna före och efter biopsi från nio patienter som genomgått en transrektal prostatabiopsi och fått en dos antibiotika profylax i samband med ingreppet.  Den optimala annealingtemperaturen bedömdes vara 60°C och den optimala  primer- och probekoncentrationen bestämdes till 1,2 µM respektive 0,4µM. Dessa koncentrationer gav lägst antal falskt positiva droppar. Den minsta detektionsnivån för S83L gyrA (EC40) var 160 kopior/ml och för vildtyps gyrA (EC108) var det 78 kopior/ml. Resultatet från valideringen visade att både vildtyps gyrA och S83L gyrA kunde detekteras och kvantifieras i rektalprover från samtliga patienter.
The most common form of cancer in men is prostate cancer, with 10,000 new deaths annually. In prostate cancer diagnosis, prostate biopsy is performed. To reduce the risk of complications in connection with biopsy, a single dose of the antibiotic drug Ciprofloxacin is given in Sweden. The proportion of bacteria that are resistant to ciprofloxacin has increased. For the detection of gene mutations that cause antibiotic resistance, droplet digital PCR (ddPCR) can be used. It is a method that provides an absolute quantification of a DNA sequence in a sample. It is based on water oil emulsion drop system.  The purpose of this study was to optimize and validate a digital droplet PCR to detect and quantify the S83L mutation in the gyrA gene from faecal samples and to compare digital droplet results from study samples with culture results from resistance determination and the ration between the S83L allele and the wild-type allele in samples taken before and after biopsy. To validate the method, samples taken before and after biopsy were used from nine patients who had undergone a transrectal prostate biopsy and received a dose of ciprofloxacin or trimethoprim in connection with the procedure.  The optimal annealing temperature was determined to be 60 °C and the optimal primer and probe concentrations were determined to be 1.2 µM and 0.4µM, respectively. These concentrations gave the lowest number of false positive droplets. The minimum detection level for S83L gyrA (EC40) was 160 copies/ml and for wild-type gyrA (EC108) it was 78 copies/ml. The results showed that both wild-type gyrA and S83L gyrA could be detected and quantified in rectal samples from all nine patients.
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Poleti, Marcelo Lupion. "Análise morfométrica, radiográfica e molecular do processo de reparo alveolar após a terapia fotodinâmica antimicrobiana em ratos Wistar." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/25/25132/tde-03072009-105031/.

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Introdução: Com o aumento da resistência bacteriana à antibioticoterapia tornou-se necessário o desenvolvimento de técnicas antimicrobianas alternativas. Assim, na busca de novas metodologias contra microrganismos, estudos usando a Terapia Fotodinâmica (TFD) antimicrobiana tem sido realizados. Objetivo: Realizar análise morfométrica, radiográfica e molecular do processo de reparo alveolar após a TFD antimicrobiana em ratos Wistar. Material e Métodos: Foram utilizados 85 ratos, divididos nos seguintes grupos: GRUPO C: Animais com alvéolo não tratado (grupo controle); GRUPO S+L: Animais com alvéolo preenchido com soro fisiológico e tratado apenas com aplicação do Laser de baixa intensidade (660 nm), com fluência de 50 J/cm2; GRUPO ATO: Animais com alvéolo tratado apenas com aplicação tópica de azul de toluidina - O (ATO) na concentração de 100 µg/mL e GRUPO ATO+L: Animais com alvéolo tratado com aplicação tópica de azul de toluidina O (ATO) na concentração de 100 µg/mL + aplicação de Laser de baixa intensidade (660 nm), com fluência de 50 J/cm2. Os animais foram sacrificados aos 6, 15 e 28 dias pós-operatório. O mapeamento térmico foi realizado em um animal para confirmar a ausência de efeito térmico do Laser sobre o local irradiado. Foram realizadas as análises microscópicas: qualitativa e quantitativa de tecido conjuntivo, tecido ósseo, coágulo sanguíneo, vaso sanguíneo, infiltrado inflamatório e espaço vazio; radiográfica: por meio do valor de pixel e dimensão fractal e molecular: quantitativa da expressão de genes envolvidos no processo de reparo: colágeno tipo I (COL-I), fator de crescimento do endotélio vascular (VEGF), runt-related transcription factor 2 (RUNX2), osteocalcina (OCN) e fosfatase alcalina (ALP), por meio da PCR Real Time. Resultados e Conclusão: Os resultados demonstraram que a TFD antimicrobiano não atuou negativamente na evolução do processo de reparo alveolar; a análise radiográfica por meio dos valores de pixel pode ser usada como indicador para avaliar a neoformação óssea no processo de reparo alveolar em ratos; a expressão de VEGF pode ser usada como marcador molecular para a angiogênese no processo de reparo alveolar em ratos e a expressão de fosfatase alcalina pode ser usada como marcador molecular nos estágios iniciais do metabolismo ósseo no processo de reparo alveolar em ratos.
Introduction: Antibiotics resistance made the development of antimicrobial alternative techniques necessary. Consequently, others alternatives such as antimicrobial photodynamic therapy (PDT) have been studied. Objective: The objectives were to perform morphometric, radiographic and molecular analyses of alveolar repair process in rats after antimicrobial photodynamic therapy. Methods: Eighty-five rats were used in this study, divided according to the following groups: C: untreated socket; S+L: socket treatment with physiologic saline solution and low intensity laser therapy (660nm - 50J/cm2); ATO: socket treatment with topic application of toluidine blue-O (100 µg/mL) and ATO+L: socket treatment with topic application of toluidine blue-O (100µg/ml) and low intensity laser therapy (660nm - 50J/cm2). The animals were sacrificed at a postoperative period of 6, 15 and 28 days. Thermal variation was carried out in an animal to confirm the absence of Laser thermal effect on irradiation area. Quantitative and qualitative microscopic analyses were performed to evaluate the connective tissue, bone tissue, blood clot, blood vessel, inflammatory infiltrate and empty space. Fractal and Pixel radiographic analysis were performed. A quantitative analysis was performed using a RealTimePCR to evaluate the genes expression involved Collagen Type I (COL-I), vascular endothelial growth factor (VEGF), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), alkaline phosphatase (ALP), in the alveolar repair process. Results and Conclusions: Based in the results, it can be concluded that antimicrobial photodynamic therapy did not act negatively in the socket bone repair evaluation. Pixel values analysis could be used as indicators to evaluate the dry socket bone neoformation. The VEGF molecular marker could be used as angiogenesis indicator to evaluate the dry socket bone neoformation as well as alkaline phosphatase as a molecular marker in the early stage of bone neoformation.
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Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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Verhaegen, Monique Elise. "Novel approaches in quantitative polymerase chain reaction." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0021/MQ52489.pdf.

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Chiou, Jeffrey Tsungshuan. "A novel capillary polymerase chain reaction machine." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/8864.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2001.
Includes bibliographical references (p. 254-268).
I built a novel prototype capillary polymerase chain reaction machine. The purpose was to perform a single reaction as fast as possible with a reaction volume - 100 nl. The PCR mix is in the form of a 1 /1 droplet that moves between three heat zones inside of a 1 mm I.D. capillary filled with mineral oil via pneumatic actuation. A laser beam waveguides down the capillary until it strikes the drop, at which point it scatters. The scatter is picked up by a series of photodiodes to provide position feedback. Due to the efficient heat transfer arrangement, the drop can transition between different temperature steps in -2 seconds, which includes both drop motion and temperature equilibration. It was extensively tested in both 10-cycle and 30-cycle PCR, including nearly 200 successful 30-cycle runs. The 30-cycle PCR was typically 74% (as high as 78%) efficient, and took only 23 minutes. This compares well with existing machines in the literature.
by Jeffrey Tsungshuan Chiou.
Ph.D.
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Clackson, Timothy Piers. "Antibody engineering using the polymerase chain reaction." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316695.

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Lantz, Pär-G. "PCR-based detection of microorganisms in complex biological samples." Lund : Dept. of Applied Microbiology, Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/39178906.html.

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Linley, M. "The detection of polymerase inhibiting lesions using the polymerase arrest polymerase chain reaction assay." Thesis, Swansea University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637924.

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There is a constant need to determine the genotoxic potential of the agents to which the human population is exposed. The stringent testing of new products is legislatively controlled and dependent on the accumulation of sufficient scientific data to allow an analysis of the risk. It is important to predetermine any risks in the workplace prior to the presentation of disease and to provide factual public information on personal exposure e.g. the risks associated with UV light. Various experimental assays have been developed to assess the genotoxicity, mutagenicity and mcarcinogenicity of given physical and chemical agents. The Polymerase Arrest- Polymerase Chain Reaction (PA-PCR) assay was employed to investigate the genotoxic effects (DNA adducts, DAN strand breaks and DNA crosslinking) of various physical and chemical agents on naked isolated DNA. The assay was modified to provide two adapted methods, which increased the sensitivity of the assay to report DNA damage at significantly lowered exposure levels. The ability of the PA-PCR assay to perform as an initial screening process for genotoxic activity was assessed and determined.
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Nebbali, M. "Human gene mapping using the polymerase chain reaction." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317395.

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Borges, Pinto Lais Izabel. "Alu-polymerase chain reaction genomic fingerprinting in neuroblastoma." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366679.

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Books on the topic "Digital polymerase chain reaction"

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Hernández-Rodríguez, Patricia, and Arlen Patricia Ramirez Gomez. Polymerase chain reaction. Rijeka: Intech, 2012.

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Mullis, Kary B., François Ferré, and Richard A. Gibbs, eds. The Polymerase Chain Reaction. Boston, MA: Birkhäuser Boston, 1994. http://dx.doi.org/10.1007/978-1-4612-0257-8.

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Oswald, Nick, and Suzanne Kennedy. PCR troubleshooting and optimization: The essential guide. Norfolk, UK: Caister Academic Press, 2011.

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Julie, Logan, Edwards Kirstin, and Saunders Nick, eds. Real-time PCR: Current technology and applications. Norfolk, UK: Caister Academic Press, 2009.

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Eeles, Rosalind A. Polymerase chain reaction (PCR): The technique and its application. Austin: R.G. Landes Co., 1993.

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Köhler, Thomas, Dirk Laßner, Harald Remke, Anne-Katrin Rost, Barbara Thamm, and Barbara Pustowoit. Quantitation of mRNA by Polymerase Chain Reaction. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-79712-5.

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Quantitative real-time PCR in applied microbiology. Norfolk, UK: Caister Academic Press, 2012.

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Malaysia. Kementerian Kesihatan. Health Technology Assessment Unit. Use of Polymerase Chain Reaction in laboratory testing. Kuala Lumpur, Malaysia: Health Technology Assessment Unit, Medical Development Division, Ministry of Health Malaysia, 2002.

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Dennis, Lo Y. M., ed. Clinical applications of PCR. Totowa, N.J: Humana Press, 1998.

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Dennis, Lo Y. M., Chiu Rossa W. K, and Chan K. C. Allen, eds. Clinical applications of PCR. 2nd ed. Totowa, N.J: Humana Press, 2006.

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Book chapters on the topic "Digital polymerase chain reaction"

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Luo, Yan, Krishnendu Chakrabarty, and Tsung-Yi Ho. "Optimization of On-Chip Polymerase Chain Reaction." In Hardware/Software Co-Design and Optimization for Cyberphysical Integration in Digital Microfluidic Biochips, 117–46. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-09006-1_5.

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Pecoraro, S. "CHAPTER 5. Digital Polymerase Chain Reaction (dPCR) – General Aspects and Applications." In Food Chemistry, Function and Analysis, 63–69. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788016025-00063.

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Lee, Katherine T. W., Vinod Gopalan, and Alfred K. Lam. "Somatic DNA Copy-Number Alterations Detection for Esophageal Adenocarcinoma Using Digital Polymerase Chain Reaction." In Methods in Molecular Biology, 195–212. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7734-5_18.

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Lee, Po-Chang, Shwu-Huey Wu, Yu-Pin Chang, and Joyce Tsung-Hsi Wang. "Innovative Applications of the Medical Information." In Digital Health Care in Taiwan, 129–52. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-05160-9_7.

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AbstractThe National Health Insurance Administration (NHIA) began a pilot telemedicine program for people in remote areas after the Ministry of Health and Welfare expanded the eligibility for telemedicine. The impact of the COVID-19 pandemic on the healthcare system also accelerated the inclusion of telemedicine in the scheme. This chapter discusses how 5G facilitates telemedicine services; for example, the virtual National Health Insurance (NHI) card could bring comprehensive medical service to more settings, such as home-based medical care, by virtualizing the identification process.The NHI played a pivotal role in Taiwan’s battle against COVID-19. Besides providing travel history, occupation, contact history, and cluster to medical providers on the NHI MediCloud systems, the NHI virtual private network system was used to distribute medical masks through contracted pharmacies in the early stage of the pandemic. Moreover, the NHIA pulled in data, including vaccination history and polymerase chain reaction test results, from the NHI database to the NHI Express app so that the public can manage their health promptly. The last part of the chapter discusses Taiwan’s successful story of opening data to the private sectors to carry out the name-based medical mask distribution system.
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Lefebvre, Cedric W., Jay P. Babich, James H. Grendell, James H. Grendell, John E. Heffner, Ronan Thibault, Claude Pichard, et al. "Polymerase Chain Reaction." In Encyclopedia of Intensive Care Medicine, 1785. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-00418-6_3252.

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Briones, Carlos. "Polymerase Chain Reaction." In Encyclopedia of Astrobiology, 1323–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-11274-4_1252.

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Dijkstra, Jeanne, and Cees P. de Jager. "Polymerase Chain Reaction." In Practical Plant Virology, 415–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72030-7_64.

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Hildebrandt, Friedhelm, and Iva Singh-Sawhney. "Polymerase Chain Reaction." In Techniques in Molecular Medicine, 207–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_14.

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Debnath, Mousumi, Godavarthi B. K. S. Prasad, and Prakash S. Bisen. "Polymerase Chain Reaction." In Molecular Diagnostics: Promises and Possibilities, 129–52. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3261-4_9.

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Pasic, Maria, Carlo Hojilla, and George M. Yousef. "Polymerase Chain Reaction." In Molecular Testing in Cancer, 39–54. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4899-8050-2_4.

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Conference papers on the topic "Digital polymerase chain reaction"

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Luo, Yan, Bhargab B. Bhattacharya, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Optimization of polymerase chain reaction on a cyberphysical digital microfluidic biochip." In 2013 IEEE/ACM International Conference on Computer-Aided Design (ICCAD). IEEE, 2013. http://dx.doi.org/10.1109/iccad.2013.6691181.

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Skvortsov, Evgeny, Lenar Valiullin, Valery Meshalkin, Rinat Mukhammadiev, Konstantin Usoltsev, and Roman Davydov. "Digital Technologies in Polymerase Chain Reaction for Analysis of Clindomycin - associated Dysbiosis." In DTMIS '20: International Scientific Conference - Digital Transformation on Manufacturing, Infrastructure and Service. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3446434.3446542.

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Li, Zipeng, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Optimization of heaters in a digital microfluidic biochip for the polymerase chain reaction." In 2014 20th International Workshop on Thermal Investigations of ICs and Systems (THERMINIC). IEEE, 2014. http://dx.doi.org/10.1109/therminic.2014.6972500.

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Li, Zipeng, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Design and optimization of 3D digital microfluidic biochips for the polymerase chain reaction." In 2015 20th Asia and South Pacific Design Automation Conference (ASP-DAC). IEEE, 2015. http://dx.doi.org/10.1109/aspdac.2015.7059002.

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Fitriyansah, Ahmad, Akhmadi Surawijaya, and Adi Indrayanto. "Pin-Constrained Digital Microfluidic Design for Polymerase Chain Reaction using Selectable Number of Stages." In 2022 IEEE 65th International Midwest Symposium on Circuits and Systems (MWSCAS). IEEE, 2022. http://dx.doi.org/10.1109/mwscas54063.2022.9859374.

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Fitriyansah, Ahmad, Akhmadi Surawijaya, and Adi Indrayanto. "Heat Transfer Analysis on Digital Microfluidic Platform for Polymerase Chain Reaction with Three Resistive Heating Elements." In 2022 9th International Conference on Information Technology, Computer, and Electrical Engineering (ICITACEE). IEEE, 2022. http://dx.doi.org/10.1109/icitacee55701.2022.9923958.

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Takeshita, T., Y. Yamamoto, M. Yamamoto-Ibusuki, T. Inao, A. Sueta, S. Fujiwara, and H. Iwase. "Abstract P6-07-07: Clinical significance ofESR1mutations using droplet digital polymerase chain reaction assay in 325 breast cancer samples." In Abstracts: Thirty-Eighth Annual CTRC-AACR San Antonio Breast Cancer Symposium; December 8-12, 2015; San Antonio, TX. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.sabcs15-p6-07-07.

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Fouillet, Y., D. Jary, A. G. Brachet, J. Berthier, R. Blervaque, L. Davous, J. M. Roux, J. L. Achard, and C. Peponnet. "EWOD Digital Microfluidics for Lab on a Chip." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96020.

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This paper presents a brief overview of Electro Wetting On Dielectric (EWOD) microdroplet actuation technology fluidic behaviour. EWOD specifics are compared with other digital microfluidics actuation modes. In particular ease of integration with complex protocols is emphasized. After reviewing the electro-wetting principle and various Electro-Hydro-Dynamic (EHD) phenomena; we compare various EWOD configurations for Lab on a Chip. Two fluid functionalities will be detailed: on-chip droplet dispensing, and mixing. We cover chip architecture and the benefits of organizing these chips as fludic microprocessors. Finally, real time PCR (Polymerase Chain Reaction) within a 64 nl droplet is described as an illustration of a biological application using EWOD.
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Pei, Shao Ning, Yi-Lun Wang, Chih-Ting Lin, and Ming C. Wu. "Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform." In 2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII). IEEE, 2013. http://dx.doi.org/10.1109/transducers.2013.6627385.

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Murad, Andre Marcio. "Abstract 565: A single institutional experience with droplet digital polymerase chain reaction (ddPCR) liquid biopsy for therapeutic decision in avanced solid tumors." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-565.

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Reports on the topic "Digital polymerase chain reaction"

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O'Leary, Timothy J., Melanie Cushion, Cynthia Wright, Thomas Fanning, and Mark Tsai. Polymerase Chain Reaction Based Diagnostic Assays for Pneumocystis Carinii. Fort Belvoir, VA: Defense Technical Information Center, March 1992. http://dx.doi.org/10.21236/ada248259.

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Black, Jonathan. Quantitative Real-Time Polymerase Chain Reaction (qPCR) of Filamentous Fungi in Carpet. Research Triangle Park, NC: RTI Press, September 2009. http://dx.doi.org/10.3768/rtipress.2009.mr.0011.0909.

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Osburn, Bennie, Marius Ianconescu, Geoffrey Akita, and Rozalia Kaufman. Rapid, Sensitive Bluetongue Virus Serogroup and Serotype Detection Using Polymerase Chain Reaction. United States Department of Agriculture, September 1995. http://dx.doi.org/10.32747/1995.7612836.bard.

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The objectives of this proposal were to enhance animal health by 1) development of a BTV serogroup diagnostic assay using polymerase chain reaction (PCR) and 2) development of a BTV serotype specific diagnostic PCR assay. A PCR assay for diagnosis of bluetongue virus (BTV) serogroup from clinical samples meeting the criteria of objective 1 was developed. This PCR assay is more sensitive than virus isolation and has been adopted by both the U.S. and Israeli collaborating laboratories of this project, as well as at least one other U.S. laboratory for routine diagnosis of BTV infection in ruminants. The basic BTV PCR protocol has also become an essential tool in BTV molecular research in both collaborating laboratories. During development of the BTV serotype specific PCR we had the opportunity to investigate a nationwide outbreak of abortions and fatal disease in dogs in the U.S. purportedly due to BTV infection via a BTV contaminated canine vaccine. The BTV serogroup PCR was integral in confirming BTV in tissues from affected dogs and in lots of the suspect vaccine. This led to the first published report of BTV infection in dogs. We discovered that BTV can produce silent persistent infection in canine cell culture. This indicated a need for more stringent screening of biologics for occult BTV infection. A novel mixed cell culture method was developed to identify occult BTV and other occult viral infection cell cultures. Serotype specific primers for PCR detection of all U.S. BTV serotypes and two Israel serotypes (BTV-2 and 10) have been evaluated and are available. A subsequent collaboration would logically include sequencing of the L2 genes of Israel BTV-4, 6 and 16, allowing incorporation of these Israel BTV serotypes into a multiplex PCR assay.
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Churchill, M. E., M. A. Gemmell, and G. E. Woloschak. Polymerase chain reaction detection of retinoblastoma gene deletions in paraffin-embedded mouse lung adenocarcinomas. Office of Scientific and Technical Information (OSTI), December 1991. http://dx.doi.org/10.2172/10173425.

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Arnett, Clint M., Giselle Rodriguez, and Stephen W. Maloney. Polymerase Chain Reaction (PCR) Analysis of Microbial Consortia on Wastewater Treatment Processes for High Explosives. Fort Belvoir, VA: Defense Technical Information Center, September 2009. http://dx.doi.org/10.21236/ada544671.

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Zhang, N. Automation and integration of polymerase chain reaction with capillary electrophoresis for high throughput genotyping and disease diagnosis. Office of Scientific and Technical Information (OSTI), February 1999. http://dx.doi.org/10.2172/348906.

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Denaro, Tracy R., Sarah K. Chelgren, Jara N. Lang, Ellen M. Strobel, Lori M. T. Balster, and Marlin D. Vangsness. DNA Isolation of Microbial Contaminants in Aviation Turbine Fuel via Traditional Polymerase Chain Reaction (PCR) and Direct PCR. Preliminary Results. Fort Belvoir, VA: Defense Technical Information Center, November 2005. http://dx.doi.org/10.21236/ada446701.

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Zhelev, Doncho V., Christopher Dupuis, Suelynn Ren, Anna Le, Mia Hunt, and Henry Gibbons. Single Nucleotide Polymorphisms (SNP)-specific Quantitative Real Time Polymerase Chain Reaction (PCR) Assay for Analyzing Competition and Emergence of the Military Hypersporulating Strains of Bacillus Atrophaeous var. Globigii. Fort Belvoir, VA: Defense Technical Information Center, September 2012. http://dx.doi.org/10.21236/ada570597.

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Burke, Allen P. Detection and Clinicopathologic Correlation of Human Immunodeficiency Virus (HIV-1) Nucleic Acids and Antigens in Reticuloendothelial and Central Nervous System Tissues, by Immunohistochemistry, in situ Hybridization, and Polymerase Chain Reaction. Fort Belvoir, VA: Defense Technical Information Center, September 1992. http://dx.doi.org/10.21236/ada259307.

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López-Valverde, Nansi, Antonio López-Valverde, Ana Suarez, Bruno Macedo de Sousa, and Juan Manuel Aragoneses. Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2021. http://dx.doi.org/10.37766/inplasy2021.10.0097.

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Review question / Objective: Is gastric helicobacter pylori infection related to periodontal diseases? Condition being studied: Therefore, the aim of this systematic review and meta-analysis was to identify and analyze clinical studies to determine the direct correlation between Helicobacter Pylori gastric infection andPeriodontal Disease. Study designs to be included: Clinical studies that provided data on Helicobacter Pylori infection in both the stomach and oral cavity, confirmed by polymerase chain reaction (PCR), rapid urease test (RUT) or enzyme-linked immunosorbent assay (ELISA). Clinical studies that associated PD with Helicobacter Pylori. The diagnosis of PD was confirmed ac-cording to the diagnostic criteria in periodontology.
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