Academic literature on the topic 'Digital polymerase chain reaction'
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Journal articles on the topic "Digital polymerase chain reaction"
Ahrberg, Christian D., Andreas Manz, and Bong Geun Chung. "Polymerase chain reaction in microfluidic devices." Lab on a Chip 16, no. 20 (2016): 3866–84. http://dx.doi.org/10.1039/c6lc00984k.
Full textFan, H. Christina, and Stephen R. Quake. "Detection of Aneuploidy with Digital Polymerase Chain Reaction." Analytical Chemistry 79, no. 19 (October 2007): 7576–79. http://dx.doi.org/10.1021/ac0709394.
Full textChang, Yi-Hsien, Gwo-Bin Lee, Fu-Chun Huang, Yi-Yu Chen, and Jr-Lung Lin. "Integrated polymerase chain reaction chips utilizing digital microfluidics." Biomedical Microdevices 8, no. 3 (May 20, 2006): 215–25. http://dx.doi.org/10.1007/s10544-006-8171-y.
Full textRoden, Anja C., Julie A. Vrana, Justin W. Koepplin, Angela E. Hudson, Andrew P. Norgan, Garrett Jenkinson, Satoko Yamaoka, et al. "Comparison of In Situ Hybridization, Immunohistochemistry, and Reverse Transcription–Droplet Digital Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Testing in Tissue." Archives of Pathology & Laboratory Medicine 145, no. 7 (March 15, 2021): 785–96. http://dx.doi.org/10.5858/arpa.2021-0008-sa.
Full textSun, Chen, Leqian Liu, Harish N. Vasudevan, Kai-Chun Chang, and Adam R. Abate. "Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction." Analytical Chemistry 93, no. 29 (July 12, 2021): 9974–79. http://dx.doi.org/10.1021/acs.analchem.1c00877.
Full textWhite, A. K., K. A. Heyries, C. Doolin, M. VanInsberghe, and C. L. Hansen. "High-Throughput Microfluidic Single-Cell Digital Polymerase Chain Reaction." Analytical Chemistry 85, no. 15 (July 24, 2013): 7182–90. http://dx.doi.org/10.1021/ac400896j.
Full textVynck, Matthijs, Wim Trypsteen, Olivier Thas, Linos Vandekerckhove, and Ward De Spiegelaere. "The Future of Digital Polymerase Chain Reaction in Virology." Molecular Diagnosis & Therapy 20, no. 5 (June 28, 2016): 437–47. http://dx.doi.org/10.1007/s40291-016-0224-1.
Full textSundberg, Scott O., Carl T. Wittwer, Chao Gao, and Bruce K. Gale. "Spinning Disk Platform for Microfluidic Digital Polymerase Chain Reaction." Analytical Chemistry 82, no. 4 (February 15, 2010): 1546–50. http://dx.doi.org/10.1021/ac902398c.
Full textByrnes, Samantha A., Tim C. Chang, Toan Huynh, Anna Astashkina, Bernhard H. Weigl, and Kevin P. Nichols. "Simple Polydisperse Droplet Emulsion Polymerase Chain Reaction with Statistical Volumetric Correction Compared with Microfluidic Droplet Digital Polymerase Chain Reaction." Analytical Chemistry 90, no. 15 (July 9, 2018): 9374–80. http://dx.doi.org/10.1021/acs.analchem.8b01988.
Full textGaňová, Martina, Haoqing Zhang, Hanliang Zhu, Marie Korabečná, and Pavel Neužil. "Multiplexed digital polymerase chain reaction as a powerful diagnostic tool." Biosensors and Bioelectronics 181 (June 2021): 113155. http://dx.doi.org/10.1016/j.bios.2021.113155.
Full textDissertations / Theses on the topic "Digital polymerase chain reaction"
Al-hashimi, Sora. "Kvantifiering med digital droplet polymerase chain reaction av gyrA-genen med och utan mutationen S83L." Thesis, Örebro universitet, Institutionen för hälsovetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-92989.
Full textThe most common form of cancer in men is prostate cancer, with 10,000 new deaths annually. In prostate cancer diagnosis, prostate biopsy is performed. To reduce the risk of complications in connection with biopsy, a single dose of the antibiotic drug Ciprofloxacin is given in Sweden. The proportion of bacteria that are resistant to ciprofloxacin has increased. For the detection of gene mutations that cause antibiotic resistance, droplet digital PCR (ddPCR) can be used. It is a method that provides an absolute quantification of a DNA sequence in a sample. It is based on water oil emulsion drop system. The purpose of this study was to optimize and validate a digital droplet PCR to detect and quantify the S83L mutation in the gyrA gene from faecal samples and to compare digital droplet results from study samples with culture results from resistance determination and the ration between the S83L allele and the wild-type allele in samples taken before and after biopsy. To validate the method, samples taken before and after biopsy were used from nine patients who had undergone a transrectal prostate biopsy and received a dose of ciprofloxacin or trimethoprim in connection with the procedure. The optimal annealing temperature was determined to be 60 °C and the optimal primer and probe concentrations were determined to be 1.2 µM and 0.4µM, respectively. These concentrations gave the lowest number of false positive droplets. The minimum detection level for S83L gyrA (EC40) was 160 copies/ml and for wild-type gyrA (EC108) it was 78 copies/ml. The results showed that both wild-type gyrA and S83L gyrA could be detected and quantified in rectal samples from all nine patients.
Poleti, Marcelo Lupion. "Análise morfométrica, radiográfica e molecular do processo de reparo alveolar após a terapia fotodinâmica antimicrobiana em ratos Wistar." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/25/25132/tde-03072009-105031/.
Full textIntroduction: Antibiotics resistance made the development of antimicrobial alternative techniques necessary. Consequently, others alternatives such as antimicrobial photodynamic therapy (PDT) have been studied. Objective: The objectives were to perform morphometric, radiographic and molecular analyses of alveolar repair process in rats after antimicrobial photodynamic therapy. Methods: Eighty-five rats were used in this study, divided according to the following groups: C: untreated socket; S+L: socket treatment with physiologic saline solution and low intensity laser therapy (660nm - 50J/cm2); ATO: socket treatment with topic application of toluidine blue-O (100 µg/mL) and ATO+L: socket treatment with topic application of toluidine blue-O (100µg/ml) and low intensity laser therapy (660nm - 50J/cm2). The animals were sacrificed at a postoperative period of 6, 15 and 28 days. Thermal variation was carried out in an animal to confirm the absence of Laser thermal effect on irradiation area. Quantitative and qualitative microscopic analyses were performed to evaluate the connective tissue, bone tissue, blood clot, blood vessel, inflammatory infiltrate and empty space. Fractal and Pixel radiographic analysis were performed. A quantitative analysis was performed using a RealTimePCR to evaluate the genes expression involved Collagen Type I (COL-I), vascular endothelial growth factor (VEGF), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), alkaline phosphatase (ALP), in the alveolar repair process. Results and Conclusions: Based in the results, it can be concluded that antimicrobial photodynamic therapy did not act negatively in the socket bone repair evaluation. Pixel values analysis could be used as indicators to evaluate the dry socket bone neoformation. The VEGF molecular marker could be used as angiogenesis indicator to evaluate the dry socket bone neoformation as well as alkaline phosphatase as a molecular marker in the early stage of bone neoformation.
Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.
Full textVerhaegen, Monique Elise. "Novel approaches in quantitative polymerase chain reaction." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0021/MQ52489.pdf.
Full textChiou, Jeffrey Tsungshuan. "A novel capillary polymerase chain reaction machine." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/8864.
Full textIncludes bibliographical references (p. 254-268).
I built a novel prototype capillary polymerase chain reaction machine. The purpose was to perform a single reaction as fast as possible with a reaction volume - 100 nl. The PCR mix is in the form of a 1 /1 droplet that moves between three heat zones inside of a 1 mm I.D. capillary filled with mineral oil via pneumatic actuation. A laser beam waveguides down the capillary until it strikes the drop, at which point it scatters. The scatter is picked up by a series of photodiodes to provide position feedback. Due to the efficient heat transfer arrangement, the drop can transition between different temperature steps in -2 seconds, which includes both drop motion and temperature equilibration. It was extensively tested in both 10-cycle and 30-cycle PCR, including nearly 200 successful 30-cycle runs. The 30-cycle PCR was typically 74% (as high as 78%) efficient, and took only 23 minutes. This compares well with existing machines in the literature.
by Jeffrey Tsungshuan Chiou.
Ph.D.
Clackson, Timothy Piers. "Antibody engineering using the polymerase chain reaction." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316695.
Full textLantz, Pär-G. "PCR-based detection of microorganisms in complex biological samples." Lund : Dept. of Applied Microbiology, Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/39178906.html.
Full textLinley, M. "The detection of polymerase inhibiting lesions using the polymerase arrest polymerase chain reaction assay." Thesis, Swansea University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637924.
Full textNebbali, M. "Human gene mapping using the polymerase chain reaction." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317395.
Full textBorges, Pinto Lais Izabel. "Alu-polymerase chain reaction genomic fingerprinting in neuroblastoma." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366679.
Full textBooks on the topic "Digital polymerase chain reaction"
Hernández-Rodríguez, Patricia, and Arlen Patricia Ramirez Gomez. Polymerase chain reaction. Rijeka: Intech, 2012.
Find full textMullis, Kary B., François Ferré, and Richard A. Gibbs, eds. The Polymerase Chain Reaction. Boston, MA: Birkhäuser Boston, 1994. http://dx.doi.org/10.1007/978-1-4612-0257-8.
Full textOswald, Nick, and Suzanne Kennedy. PCR troubleshooting and optimization: The essential guide. Norfolk, UK: Caister Academic Press, 2011.
Find full textJulie, Logan, Edwards Kirstin, and Saunders Nick, eds. Real-time PCR: Current technology and applications. Norfolk, UK: Caister Academic Press, 2009.
Find full textEeles, Rosalind A. Polymerase chain reaction (PCR): The technique and its application. Austin: R.G. Landes Co., 1993.
Find full textKöhler, Thomas, Dirk Laßner, Harald Remke, Anne-Katrin Rost, Barbara Thamm, and Barbara Pustowoit. Quantitation of mRNA by Polymerase Chain Reaction. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-79712-5.
Full textQuantitative real-time PCR in applied microbiology. Norfolk, UK: Caister Academic Press, 2012.
Find full textMalaysia. Kementerian Kesihatan. Health Technology Assessment Unit. Use of Polymerase Chain Reaction in laboratory testing. Kuala Lumpur, Malaysia: Health Technology Assessment Unit, Medical Development Division, Ministry of Health Malaysia, 2002.
Find full textDennis, Lo Y. M., ed. Clinical applications of PCR. Totowa, N.J: Humana Press, 1998.
Find full textDennis, Lo Y. M., Chiu Rossa W. K, and Chan K. C. Allen, eds. Clinical applications of PCR. 2nd ed. Totowa, N.J: Humana Press, 2006.
Find full textBook chapters on the topic "Digital polymerase chain reaction"
Luo, Yan, Krishnendu Chakrabarty, and Tsung-Yi Ho. "Optimization of On-Chip Polymerase Chain Reaction." In Hardware/Software Co-Design and Optimization for Cyberphysical Integration in Digital Microfluidic Biochips, 117–46. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-09006-1_5.
Full textPecoraro, S. "CHAPTER 5. Digital Polymerase Chain Reaction (dPCR) – General Aspects and Applications." In Food Chemistry, Function and Analysis, 63–69. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788016025-00063.
Full textLee, Katherine T. W., Vinod Gopalan, and Alfred K. Lam. "Somatic DNA Copy-Number Alterations Detection for Esophageal Adenocarcinoma Using Digital Polymerase Chain Reaction." In Methods in Molecular Biology, 195–212. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7734-5_18.
Full textLee, Po-Chang, Shwu-Huey Wu, Yu-Pin Chang, and Joyce Tsung-Hsi Wang. "Innovative Applications of the Medical Information." In Digital Health Care in Taiwan, 129–52. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-05160-9_7.
Full textLefebvre, Cedric W., Jay P. Babich, James H. Grendell, James H. Grendell, John E. Heffner, Ronan Thibault, Claude Pichard, et al. "Polymerase Chain Reaction." In Encyclopedia of Intensive Care Medicine, 1785. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-00418-6_3252.
Full textBriones, Carlos. "Polymerase Chain Reaction." In Encyclopedia of Astrobiology, 1323–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-11274-4_1252.
Full textDijkstra, Jeanne, and Cees P. de Jager. "Polymerase Chain Reaction." In Practical Plant Virology, 415–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72030-7_64.
Full textHildebrandt, Friedhelm, and Iva Singh-Sawhney. "Polymerase Chain Reaction." In Techniques in Molecular Medicine, 207–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_14.
Full textDebnath, Mousumi, Godavarthi B. K. S. Prasad, and Prakash S. Bisen. "Polymerase Chain Reaction." In Molecular Diagnostics: Promises and Possibilities, 129–52. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3261-4_9.
Full textPasic, Maria, Carlo Hojilla, and George M. Yousef. "Polymerase Chain Reaction." In Molecular Testing in Cancer, 39–54. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4899-8050-2_4.
Full textConference papers on the topic "Digital polymerase chain reaction"
Luo, Yan, Bhargab B. Bhattacharya, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Optimization of polymerase chain reaction on a cyberphysical digital microfluidic biochip." In 2013 IEEE/ACM International Conference on Computer-Aided Design (ICCAD). IEEE, 2013. http://dx.doi.org/10.1109/iccad.2013.6691181.
Full textSkvortsov, Evgeny, Lenar Valiullin, Valery Meshalkin, Rinat Mukhammadiev, Konstantin Usoltsev, and Roman Davydov. "Digital Technologies in Polymerase Chain Reaction for Analysis of Clindomycin - associated Dysbiosis." In DTMIS '20: International Scientific Conference - Digital Transformation on Manufacturing, Infrastructure and Service. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3446434.3446542.
Full textLi, Zipeng, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Optimization of heaters in a digital microfluidic biochip for the polymerase chain reaction." In 2014 20th International Workshop on Thermal Investigations of ICs and Systems (THERMINIC). IEEE, 2014. http://dx.doi.org/10.1109/therminic.2014.6972500.
Full textLi, Zipeng, Tsung-Yi Ho, and Krishnendu Chakrabarty. "Design and optimization of 3D digital microfluidic biochips for the polymerase chain reaction." In 2015 20th Asia and South Pacific Design Automation Conference (ASP-DAC). IEEE, 2015. http://dx.doi.org/10.1109/aspdac.2015.7059002.
Full textFitriyansah, Ahmad, Akhmadi Surawijaya, and Adi Indrayanto. "Pin-Constrained Digital Microfluidic Design for Polymerase Chain Reaction using Selectable Number of Stages." In 2022 IEEE 65th International Midwest Symposium on Circuits and Systems (MWSCAS). IEEE, 2022. http://dx.doi.org/10.1109/mwscas54063.2022.9859374.
Full textFitriyansah, Ahmad, Akhmadi Surawijaya, and Adi Indrayanto. "Heat Transfer Analysis on Digital Microfluidic Platform for Polymerase Chain Reaction with Three Resistive Heating Elements." In 2022 9th International Conference on Information Technology, Computer, and Electrical Engineering (ICITACEE). IEEE, 2022. http://dx.doi.org/10.1109/icitacee55701.2022.9923958.
Full textTakeshita, T., Y. Yamamoto, M. Yamamoto-Ibusuki, T. Inao, A. Sueta, S. Fujiwara, and H. Iwase. "Abstract P6-07-07: Clinical significance ofESR1mutations using droplet digital polymerase chain reaction assay in 325 breast cancer samples." In Abstracts: Thirty-Eighth Annual CTRC-AACR San Antonio Breast Cancer Symposium; December 8-12, 2015; San Antonio, TX. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.sabcs15-p6-07-07.
Full textFouillet, Y., D. Jary, A. G. Brachet, J. Berthier, R. Blervaque, L. Davous, J. M. Roux, J. L. Achard, and C. Peponnet. "EWOD Digital Microfluidics for Lab on a Chip." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96020.
Full textPei, Shao Ning, Yi-Lun Wang, Chih-Ting Lin, and Ming C. Wu. "Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform." In 2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII). IEEE, 2013. http://dx.doi.org/10.1109/transducers.2013.6627385.
Full textMurad, Andre Marcio. "Abstract 565: A single institutional experience with droplet digital polymerase chain reaction (ddPCR) liquid biopsy for therapeutic decision in avanced solid tumors." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-565.
Full textReports on the topic "Digital polymerase chain reaction"
O'Leary, Timothy J., Melanie Cushion, Cynthia Wright, Thomas Fanning, and Mark Tsai. Polymerase Chain Reaction Based Diagnostic Assays for Pneumocystis Carinii. Fort Belvoir, VA: Defense Technical Information Center, March 1992. http://dx.doi.org/10.21236/ada248259.
Full textBlack, Jonathan. Quantitative Real-Time Polymerase Chain Reaction (qPCR) of Filamentous Fungi in Carpet. Research Triangle Park, NC: RTI Press, September 2009. http://dx.doi.org/10.3768/rtipress.2009.mr.0011.0909.
Full textOsburn, Bennie, Marius Ianconescu, Geoffrey Akita, and Rozalia Kaufman. Rapid, Sensitive Bluetongue Virus Serogroup and Serotype Detection Using Polymerase Chain Reaction. United States Department of Agriculture, September 1995. http://dx.doi.org/10.32747/1995.7612836.bard.
Full textChurchill, M. E., M. A. Gemmell, and G. E. Woloschak. Polymerase chain reaction detection of retinoblastoma gene deletions in paraffin-embedded mouse lung adenocarcinomas. Office of Scientific and Technical Information (OSTI), December 1991. http://dx.doi.org/10.2172/10173425.
Full textArnett, Clint M., Giselle Rodriguez, and Stephen W. Maloney. Polymerase Chain Reaction (PCR) Analysis of Microbial Consortia on Wastewater Treatment Processes for High Explosives. Fort Belvoir, VA: Defense Technical Information Center, September 2009. http://dx.doi.org/10.21236/ada544671.
Full textZhang, N. Automation and integration of polymerase chain reaction with capillary electrophoresis for high throughput genotyping and disease diagnosis. Office of Scientific and Technical Information (OSTI), February 1999. http://dx.doi.org/10.2172/348906.
Full textDenaro, Tracy R., Sarah K. Chelgren, Jara N. Lang, Ellen M. Strobel, Lori M. T. Balster, and Marlin D. Vangsness. DNA Isolation of Microbial Contaminants in Aviation Turbine Fuel via Traditional Polymerase Chain Reaction (PCR) and Direct PCR. Preliminary Results. Fort Belvoir, VA: Defense Technical Information Center, November 2005. http://dx.doi.org/10.21236/ada446701.
Full textZhelev, Doncho V., Christopher Dupuis, Suelynn Ren, Anna Le, Mia Hunt, and Henry Gibbons. Single Nucleotide Polymorphisms (SNP)-specific Quantitative Real Time Polymerase Chain Reaction (PCR) Assay for Analyzing Competition and Emergence of the Military Hypersporulating Strains of Bacillus Atrophaeous var. Globigii. Fort Belvoir, VA: Defense Technical Information Center, September 2012. http://dx.doi.org/10.21236/ada570597.
Full textBurke, Allen P. Detection and Clinicopathologic Correlation of Human Immunodeficiency Virus (HIV-1) Nucleic Acids and Antigens in Reticuloendothelial and Central Nervous System Tissues, by Immunohistochemistry, in situ Hybridization, and Polymerase Chain Reaction. Fort Belvoir, VA: Defense Technical Information Center, September 1992. http://dx.doi.org/10.21236/ada259307.
Full textLópez-Valverde, Nansi, Antonio López-Valverde, Ana Suarez, Bruno Macedo de Sousa, and Juan Manuel Aragoneses. Association of gastric infection and periodontal disease through Helicobacter pylori as a common denominator: A systematic review and meta-analysi. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2021. http://dx.doi.org/10.37766/inplasy2021.10.0097.
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