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1

Taggar, Monica Sachdeva. "Insect cellulolytic enzymes: Novel sources for degradation of lignocellulosic biomass." Journal of Applied and Natural Science 7, no. 2 (December 1, 2015): 625–30. http://dx.doi.org/10.31018/jans.v7i2.656.

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Alternative and renewable fuels derived from lignocellulosic biomass offer the potential to reduce our dependence on fossil fuels and mitigate global climate change. Cellulose is one of the major structural components in all lignocellulosic wastes and enzymatic depolymerization of cellulose by cellulases is an essential step in bio-ethanol production. Wood-degrading insects are potential source of biochemical catalysts for converting wood lignocellulose into biofuels. Cellulose digestion has been demonstrated in more than 20 insect families representing ten distinct insect orders. Termite guts been have considered as the “world’s smallest bioreactors” since they digest a significant proportion of cellulose (74-99%) and hemicellulose (65-87%) components of lignocelluloses they ingest. The lower termites harbor protistan symbionts in hindgut whereas higher termites lack these in the hind gut. Studies on cellulose digestion in termites and other insects with reference to ligno-cellulose degrading enzymes have been well focused in this review. The studies on insect cellulolytic systems can lead to the discovery of a variety of novel biocatalysts and genes that encode them, as well as associated unique mechanisms for efficient biomass conversion into biofuels.
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Li, Renfei, Wenbing Tan, Xinyu Zhao, Qiuling Dang, Qidao Song, Beidou Xi, and Xiaohui Zhang. "Evaluation on the Methane Production Potential of Wood Waste Pretreated with NaOH and Co-Digested with Pig Manure." Catalysts 9, no. 6 (June 17, 2019): 539. http://dx.doi.org/10.3390/catal9060539.

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Wood waste generated during the tree felling and processing is a rich, green, and renewable lignocellulosic biomass. However, an effective method to apply wood waste in anaerobic digestion is lacking. The high carbon to nitrogen (C/N) ratio and rich lignin content of wood waste are the major limiting factors for high biogas production. NaOH pre-treatment for lignocellulosic biomass is a promising approach to weaken the adverse effect of complex crystalline cellulosic structure on biogas production in anaerobic digestion, and the synergistic integration of lignocellulosic biomass with low C/N ratio biomass in anaerobic digestion is a logical option to balance the excessive C/N ratio. Here, we assessed the improvement of methane production of wood waste in anaerobic digestion by NaOH pretreatment, co-digestion technique, and their combination. The results showed that the methane yield of the single digestion of wood waste was increased by 38.5% after NaOH pretreatment compared with the untreated wood waste. The methane production of the co-digestion of wood waste and pig manure was higher than that of the single digestion of wood waste and had nonsignificant difference with the single-digestion of pig manure. The methane yield of the co-digestion of wood waste pretreated with NaOH and pig manure was increased by 75.8% than that of the untreated wood waste. The findings indicated that wood waste as a sustainable biomass source has considerable potential to achieve high biogas production in anaerobic digestion.
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3

Adney, William S., Christopher J. Rivard, Ming Shiang, and Michael E. Himmel. "Anaerobic digestion of lignocellulosic biomass and wastes." Applied Biochemistry and Biotechnology 30, no. 2 (August 1991): 165–83. http://dx.doi.org/10.1007/bf02921684.

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Rahimi-Ajdadi, Fatemeh, and Masoomeh Esmaili. "Effective Pre-Treatments for Enhancement of Biodegradation of Agricultural Lignocellulosic Wastes in Anaerobic Digestion – A Review." Acta Technologica Agriculturae 23, no. 3 (September 1, 2020): 105–10. http://dx.doi.org/10.2478/ata-2020-0017.

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AbstractAgricultural crop residues like stems, straws and leaves are valuable resources for biofuel production, especially methane, due to anaerobic digestion. Biogas from agricultural lignocellulosic wastes is capable of attaining sustainable energy yields without environmental pollution. Farmers in many developing countries burn these wastes throughout their fields, imposing environmental hazard due to emission of greenhouse gases. The main problem in this field is the recalcitrance of the agricultural lignocellulose waste that limits its enzymatic degradation and hydrolysis efficiency and consequently decreases biogas production. Therefore, efficient pre-treatments prior to anaerobic digestion are essential. Various pre-treatment methods are used for increasing the anaerobic digestibility of lignocellulose biomass, such as physical (mechanical, thermal, etc.), chemical, biological and combined pre-treatments. This paper reviews different pre-treatments used in anaerobic digestion for the agricultural lignocellulosic wastes and explains the advantages and disadvantages of each. The most frequently used pre-treatments for main agricultural wastes in process of biogas production are also introduced.
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5

Gnanambal, Venkatachalam Sundaresan, and Krishnaswamy Swaminathan. "Biogas production from renewable lignocellulosic biomass." International Journal of Environment 4, no. 2 (June 3, 2015): 341–47. http://dx.doi.org/10.3126/ije.v4i2.12662.

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Effect of raw and biologically treated lignocellulosic biomass using cow dung slurry for biogas production is reported. Biomass is an energy source. Water containing biomass such as sewage sludge, cow dung slurry and lignocellulosic waste, has several important advantages and one of the key feature is renewability. Cow dung slurry has the potential to produce large amounts of biogas. Four categories of bacteria viz., hydrolytic, fermentative, fermentative acidogenic and acidogenic-methanogenic bacteria are involved in the production of biogas. The different characteristics of the cow dung slurry were determined according to standard methods. Hemicellulose, cellulose and lignin content of the lignocellulosic waste were also determined in our earlier studies. The substrates were digested under anaerobic condition for 5 days. The total biogas and methane produced during anaerobic digestion were estimated on 5th day. The total biogas produced during digestion was estimated by water displacement method. Biological methane production was estimated by using Saccharometer. DOI: http://dx.doi.org/10.3126/ije.v4i2.12662 International Journal of Environment Vol.4(2) 2015: 341-347
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6

Ahmed, Banafsha, Kaoutar Aboudi, Vinay Kumar Tyagi, Carlos José Álvarez-Gallego, Luis Alberto Fernández-Güelfo, Luis Isidoro Romero-García, and A. A. Kazmi. "Improvement of Anaerobic Digestion of Lignocellulosic Biomass by Hydrothermal Pretreatment." Applied Sciences 9, no. 18 (September 13, 2019): 3853. http://dx.doi.org/10.3390/app9183853.

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Lignocellulosic biomass, comprising of cellulose, hemicellulose, and lignin, is a difficult-to-degrade substrate when subjected to anaerobic digestion. Hydrothermal pretreatment of lignocellulosic biomass could enhance the process performance by increasing the generation of methane, hydrogen, and bioethanol. The recalcitrants (furfurals, and 5-HMF) could be formed at high temperatures during hydrothermal pretreatment of lignocellulosic biomass, which may hinder the process performance. However, the detoxification process involving the use of genetically engineered microbes may be a promising option to reduce the toxic effects of inhibitors. The key challenge lies in the scaleup of the hydrothermal process, mainly due to necessity of upholding high temperature in sizeable reactors, which may demand high capital and operational costs. Thus, more efforts should be towards the techno-economic feasibility of hydrothermal pre-treatment at full scale.
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7

Agregán, Rubén, José M. Lorenzo, Manoj Kumar, Mohammad Ali Shariati, Muhammad Usman Khan, Abid Sarwar, Muhammad Sultan, Maksim Rebezov, and Muhammad Usman. "Anaerobic Digestion of Lignocellulose Components: Challenges and Novel Approaches." Energies 15, no. 22 (November 10, 2022): 8413. http://dx.doi.org/10.3390/en15228413.

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The reuse of lignocellulosic biomaterials as a source of clean energy has been explored in recent years due to the large amount of waste that involves human activities, such as those related to agriculture and food. The anaerobic digestion (AD) of plant-based biomass for bioenergy production poses a series of challenges that new technologies are attempting to solve. An improved decomposition of recalcitrant lignocellulose together with an increase in biogas production yield are the main objectives of these new approaches, which also seek the added value of being environmentally friendly. Recent research has reported significant progress in this regard, offering promising outcomes on the degradation of lignocellulose and its subsequent transformation into biomethane by specialized anaerobic microorganisms, overcoming the drawbacks inherent to the process and improving the yield of methane production. The future of the agri–food industry seems to be heading towards the implementation of a circular economy through the introduction of strategies based on the optimized use of lignocellulosic residues as a source of clean and sustainable energy.
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8

Sawatdeenarunat, Chayanon, K. C. Surendra, Devin Takara, Hans Oechsner, and Samir Kumar Khanal. "Anaerobic digestion of lignocellulosic biomass: Challenges and opportunities." Bioresource Technology 178 (February 2015): 178–86. http://dx.doi.org/10.1016/j.biortech.2014.09.103.

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9

Piccitto, Alessandra, Danilo Scordia, Sebastiano Andrea Corinzia, Salvatore Luciano Cosentino, and Giorgio Testa. "Advanced Biomethane Production from Biologically Pretreated Giant Reed under Different Harvest Times." Agronomy 12, no. 3 (March 16, 2022): 712. http://dx.doi.org/10.3390/agronomy12030712.

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Increasing energy demands and fossil fuel consumption causing global warming has motivated research to find alternative energy sources such as biofuels. Giant reed (Arundo donax L.), a lignocellulosic, perennial, rhizomatous grass has been proposed as an important bioenergy crop for advanced biofuel in the Mediterranean area. Anaerobic digestion for advanced biomethane seems to be a promising approach. However, the presence of lignin in lignocellulosic biomass represents the main obstacle to its production (due to its recalcitrance). Thus, to use effectively lignocellulosic biomass in anaerobic digestion, one or more pretreatment steps are needed to aid microorganisms access to the plant cell wall. To this end, the present study investigated the effect of fungal pretreatment of giant reeds obtained from two different harvesting time (autumn and winter) on biomethane production by anaerobic digestion using two white rot fungi (Pleurotus ostreatus and Irpex lactus, respectively). The highest biomass lignin degradation after 30 days incubation with P. ostreatus in both autumn (27.1%) and winter (31.5%) harvest time. P. ostreatus pretreatment showed promising results for anaerobic digestion of giant reed achieving a cumulative yield of 130.9 NmL g−1 VS for the winter harvest, whereas I. lacteus showed a decrease in methane yield as compared with the untreated biomass (77.4 NmL g−1 VS and 73.3 NmL g−1 VS for winter and autumn harvest, respectively). I. lacteus pretreatment resulted in a loss of both holocellulose and lignin, indicating that this strain was less selective than P. ostreatus. Further studies are necessary to identify white rot fungi more suitable to lignocellulosic biomass and optimize biological pretreatment conditions to reduce its duration.
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10

Takizawa, Shuhei, Yasunori Baba, Chika Tada, Yasuhiro Fukuda, and Yutaka Nakai. "Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition." Journal of Material Cycles and Waste Management 22, no. 3 (January 6, 2020): 656–63. http://dx.doi.org/10.1007/s10163-019-00957-8.

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AbstractRumen fluid has been applied to lignocellulosic biomass digest for methane production, and various feed supplements have been shown to improve ruminant digestion of lignocellulosic biomass. Therefore, we investigated the effects of sodium dodecyl sulfate (SDS) on the pretreatment of lignocellulosic biomass with rumen fluid and subsequent methane fermentation. SDS was mixed with rumen fluid at concentrations of 0.1, 0.2, 0.4, and 0.8 g/L. After SDS addition, the waste paper was pretreated with rumen fluid at 37 °C for 6 h. SDS addition decreased the number of surviving rumen ciliates after pretreatment. SDS addition increased the dissolved chemical oxygen demand during pretreatment; however, SDS addition did not increase the volatile fatty acid concentration. After pretreatment, batch methane fermentation of pretreated waste paper was performed at 35 °C for 45 days. SDS addition at 0.1 and 0.2 g/L shortened the waste paper digestion time and enhanced methane gas production compared to the control. By contrast, SDS addition at 0.4 and 0.8 g/L remarkably inhibited methane production from waste paper. These findings suggest that low concentrations of SDS can improve the efficiency of lignocellulosic biomass pretreatment with rumen fluid, and can enhance methane production from waste paper.
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11

HIDALGO BARRIO, MARIA DOLORES, JUAN CASTRO BUSTAMANTE, JESUS MARIA MARTIN MARROQUIN, FRANCISCO CORONA ENCINAS, and SERGIO SANZ BEDATE. "EFFECT OF DIFFERENT PHYSICAL PRETREATMENT STRATEGIES ON THE BIODEGRADABILITY OF LIGNOCELLULOSIC MATERIALS." DYNA 97, no. 2 (March 1, 2022): 150–55. http://dx.doi.org/10.6036/10179.

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When lignocellulosic biomass is used as a substrate in anaerobic digestion processes, a pretreatment stage is always required to break the hard structure of the material and facilitate the attack of microorganisms and, with it, their degradation. Numerous methods have been developed to pretreat lignocellulosic biomass. Four of them: torrefaction, cavitation, pelletizing and extrusion have been comparatively evaluated in this article as ways to increase methane production through anaerobic digestion of two raw materials with different lignin content: barley straw and vine shoot. In addition, it was examined how these pretreatments and the nature of the feedstock influence the volatile fatty acid profiles that are generated during the digestion process. The cavitation of biomass milled at 0.25 mm was revealed as the most efficient pretreatment among those tested, increasing by 360% and 240%, respectively, the methane production for vine shoots and barley straw.
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12

Ibro, Mohammed Kelif, Venkata Ramayya Ancha, and Dejene Beyene Lemma. "Impacts of Anaerobic Co-Digestion on Different Influencing Parameters: A Critical Review." Sustainability 14, no. 15 (July 31, 2022): 9387. http://dx.doi.org/10.3390/su14159387.

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Lignocellulosic feedstocks are year-round, available bio-residues that are the right candidates for counteracting the energy crises and global warming facing the world today. However, lignin leads to a slow hydrolysis rate and is a major bottleneck for biogas production via anaerobic digestion. Anaerobic co-digestion (AcoD) is an economical method available, which overcomes the limitation of a single feedstock’s properties in an anaerobic digestion process. This paper critically reviews the impacts of co-digestion on lignocellulosic biomass degradation, process stability, various working parameters, and microbial activities that improve methane yields. A combination of compatible substrates is chosen to improve the biomethane yield and conversion rate of organic matter. AcoD is a promising method in the delignification of lignocellulosic biomass as an acid pretreatment. Ultimate practices to control the impact of co-digestion on system performances include co-feed selection, in terms of both carbon-to-nitrogen (C/N) and mixing ratios, and other operating conditions. A detailed analysis is performed using data reported in the recent past to assess the sensitivity of influencing parameters on the resultant biogas yield. For the investigators motivated by the basic principles of AcoD technology, this review paper generates baseline data for further research work around co-digestion.
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13

Wang, Xuemei, Shikun Cheng, Zifu Li, Yu Men, and Jiajun Wu. "Impacts of Cellulase and Amylase on Enzymatic Hydrolysis and Methane Production in the Anaerobic Digestion of Corn Straw." Sustainability 12, no. 13 (July 6, 2020): 5453. http://dx.doi.org/10.3390/su12135453.

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The impacts of enzyme pre-treatments on anaerobic digestion of lignocellulosic biomass were explored by using corn straw as a substrate for enzyme pre-treatment and anaerobic digestion and by utilizing starch and microcrystalline cellulose as substrates for comparative analysis. The cellulase pre-treatment effectively improved the enzymatic hydrolysis of cellulose, decreased the crystallinity, and consequently showed 33.2% increase in methane yield. The methane yield of starch increased by 16.0% through amylase pre-treatment. However, when the substrate was corn straw, both the efficiencies of enzymes and methane production were markedly reduced by the lignocellulosic structure. The corn straw’s methane yields were 277.6 and 242.4 mL·CH4/g·VS with cellulase and amylase pre-treatment, respectively, which was 11.7% and 27.9% higher than that of the untreated corn straw. It may imply that the lignocellulose should be broken up firstly, enzyme pre-treatments could have great potentials when combined with other methods.
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14

Ferdeș, Mariana, Mirela Nicoleta Dincă, Georgiana Moiceanu, Bianca Ștefania Zăbavă, and Gigel Paraschiv. "Microorganisms and Enzymes Used in the Biological Pretreatment of the Substrate to Enhance Biogas Production: A Review." Sustainability 12, no. 17 (September 3, 2020): 7205. http://dx.doi.org/10.3390/su12177205.

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The pretreatment of lignocellulosic biomass (LC biomass) prior to the anaerobic digestion (AD) process is a mandatory step to improve feedstock biodegradability and biogas production. An important potential is provided by lignocellulosic materials since lignocellulose represents a major source for biogas production, thus contributing to the environmental sustainability. The main limitation of LC biomass for use is its resistant structure. Lately, biological pretreatment (BP) gained popularity because they are eco-friendly methods that do not require chemical or energy input. A large number of bacteria and fungi possess great ability to convert high molecular weight compounds from the substrate into lower mass compounds due to the synthesis of microbial extracellular enzymes. Microbial strains isolated from various sources are used singly or in combination to break down the recalcitrant polymeric structures and thus increase biogasgeneration. Enzymatic treatment of LC biomass depends mainly on enzymes like hemicellulases and cellulases generated by microorganisms. The articles main purpose is to provide an overview regarding the enzymatic/biological pretreatment as one of the most potent techniques for enhancing biogas production.
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Paul, Subhash, and Animesh Dutta. "Challenges and opportunities of lignocellulosic biomass for anaerobic digestion." Resources, Conservation and Recycling 130 (March 2018): 164–74. http://dx.doi.org/10.1016/j.resconrec.2017.12.005.

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16

Saady, Noori M. Cata, and Daniel I. Massé. "Psychrophilic anaerobic digestion of lignocellulosic biomass: A characterization study." Bioresource Technology 142 (August 2013): 663–71. http://dx.doi.org/10.1016/j.biortech.2013.05.089.

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17

Terry, Stephanie A., Ajay Badhan, Yuxi Wang, Alexandre V. Chaves, and Tim A. McAllister. "Fibre digestion by rumen microbiota — a review of recent metagenomic and metatranscriptomic studies." Canadian Journal of Animal Science 99, no. 4 (December 1, 2019): 678–92. http://dx.doi.org/10.1139/cjas-2019-0024.

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Plant biomass is the most abundant renewable resource on the planet, and the biopolymers of lignocellulose are the foundation of ruminant production systems. Optimizing the saccharification of lignocellulosic feeds is a crucial step in their bioconversion to ruminant protein. Plant cell walls are chemically heterogeneous structures that have evolved to provide structural support and protection to the plant. Ruminants are the most efficient digesters of lignocellulose due to a rich array of bacteria, archaea, fungi, and protozoa within the rumen and lower digestive tract. Metagenomic and metatranscriptomic studies have enhanced the current understanding of the composition, diversity, and function of the rumen microbiome. There is particular interest in identifying the carbohydrate-active enzymes responsible for the ruminal degradation of plant biomass. Understanding the roles of cellulosomes- and polysaccharide-utilising loci in ruminal fibre degradation could provide insight into strategies to enhance forage utilisation by ruminants. Despite advancements in “omics” technology, the majority of rumen microorganisms are still uncharacterised, and their ability to act synergistically is still not understood. By advancing our current knowledge of rumen fibre digestion, there may be opportunity to further improve the productive performance of ruminants fed forage diets.
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Romero-García, Luis Isidoro, Carlos José Álvarez-Gallego, and Luis Alberto Fernández-Güelfo. "Editorial of the Special Issue “Anaerobic Co-Digestion of Lignocellulosic Wastes”." Applied Sciences 10, no. 21 (October 22, 2020): 7399. http://dx.doi.org/10.3390/app10217399.

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19

Chukwuma, Ogechukwu Bose, Mohd Rafatullah, Husnul Azan Tajarudin, and Norli Ismail. "A Review on Bacterial Contribution to Lignocellulose Breakdown into Useful Bio-Products." International Journal of Environmental Research and Public Health 18, no. 11 (June 3, 2021): 6001. http://dx.doi.org/10.3390/ijerph18116001.

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Discovering novel bacterial strains might be the link to unlocking the value in lignocellulosic bio-refinery as we strive to find alternative and cleaner sources of energy. Bacteria display promise in lignocellulolytic breakdown because of their innate ability to adapt and grow under both optimum and extreme conditions. This versatility of bacterial strains is being harnessed, with qualities like adapting to various temperature, aero tolerance, and nutrient availability driving the use of bacteria in bio-refinery studies. Their flexible nature holds exciting promise in biotechnology, but despite recent pointers to a greener edge in the pretreatment of lignocellulose biomass and lignocellulose-driven bioconversion to value-added products, the cost of adoption and subsequent scaling up industrially still pose challenges to their adoption. However, recent studies have seen the use of co-culture, co-digestion, and bioengineering to overcome identified setbacks to using bacterial strains to breakdown lignocellulose into its major polymers and then to useful products ranging from ethanol, enzymes, biodiesel, bioflocculants, and many others. In this review, research on bacteria involved in lignocellulose breakdown is reviewed and summarized to provide background for further research. Future perspectives are explored as bacteria have a role to play in the adoption of greener energy alternatives using lignocellulosic biomass.
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Bayané, Ali, and Serge R. Guiot. "Animal digestive strategies versus anaerobic digestion bioprocesses for biogas production from lignocellulosic biomass." Reviews in Environmental Science and Bio/Technology 10, no. 1 (June 12, 2010): 43–62. http://dx.doi.org/10.1007/s11157-010-9209-4.

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Shitophyta, Lukhi Mulia, Zahra Lintang Cahyaningtyas, Nurul Aulia Syifa, and Firda Mahira Alfiata Chusna. "Various Types of Acids on Pretreatment of Corn Stover for Enhancing Biogas Yield." JTERA (Jurnal Teknologi Rekayasa) 7, no. 2 (December 31, 2022): 275. http://dx.doi.org/10.31544/jtera.v7.i2.2022.275-280.

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Biogas production from lignocellulosic biomass has gained attention in the development of renewable fuels. Corn stover belongs to lignocellulosic biomass. Pretreatment is needed to help the digestion of biomass due to its lignocellulosic recalcitrance. This study aims to compare the different types of acids for enhancing biogas production. The experiment was carried out in a 1 L batch digester at room temperature with different acids of HCl, H2SO4, and C2H2O4 at concentrations of 0%, 5%, 10%, and 15%. The acids pretreatment was performed for 24 hr. Results show that pretreatment of C2H2O4 has a positive impact on increasing biogas yield. The highest cumulative yield of 580.8 mL/gVS is obtained at 15% C2H2O4. The increase in acid concentrations decreases the initial pH value. The pH value below 6 reduces biogas yield.
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Kamperidou, Vasiliki, and Paschalina Terzopoulou. "Anaerobic Digestion of Lignocellulosic Waste Materials." Sustainability 13, no. 22 (November 19, 2021): 12810. http://dx.doi.org/10.3390/su132212810.

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Nowadays, the climate mitigation policies of EU promote the energy production based on renewable resources. Anaerobic digestion (AD) constitutes a biochemical process that can convert lignocellulosic materials into biogas, used for chemical products isolation or energy production, in the form of electricity, heat or fuels. Such practices are accompanied by several economic, environmental and climatic benefits. The method of AD is an effective method of utilization of several different low-value and negative-cost highly available materials of residual character, such as the lignocellulosic wastes coming from forest, agricultural or marine biomass utilization processes, in order to convert them into directly usable energy. Lignin depolymerization remains a great challenge for the establishment of a full scale process for AD of lignin waste. This review analyzes the method of anaerobic digestion (biomethanation), summarizes the technology and standards involved, the progress achieved so far on the depolymerization/pre-treatment methods of lignocellulosic bio-wastes and the respective residual byproducts coming from industrial processes, aiming to their conversion into energy and the current attempts concerning the utilization of the produced biogas. Substrates’ mechanical, physical, thermal, chemical, and biological pretreatments or a combination of those before biogas production enhance the hydrolysis stage efficiency and, therefore, biogas generation. AD systems are immensely expanding globally, especially in Europe, meeting the high demands of humans for clean energy.
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Singh, Richa, Meenu Hans, Sachin Kumar, and Yogender Kumar Yadav. "Thermophilic Anaerobic Digestion: An Advancement towards Enhanced Biogas Production from Lignocellulosic Biomass." Sustainability 15, no. 3 (January 18, 2023): 1859. http://dx.doi.org/10.3390/su15031859.

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Thermophilic anaerobic digestion (TAD) technology has been adopted worldwide mainly due to it being a pathogen-free process in addition to the enhanced biogas yield and short hydraulic retention time (HRT). Taking the high metabolic rate of the thermophilic microbial community with highly efficient enzymatic systems into consideration, thermophiles are being widely explored as efficient inocula for lignocellulosic biomass (LCB) degradation and improved biomethane production. The advantages of TAD over mesophilic anaerobic digestion (MAD), including improved kinetics, efficient degradation of organic matter, and economic and environmental sustainability, make it one of the best strategies to be operated at moderately high temperatures. This review sheds light on the relevant role of thermophilic microorganisms as inocula in the anaerobic digestion of organic matter and factors affecting the overall process stability at high temperatures. Further, the discussion explains the strategies for enhancing the efficiency of thermophilic anaerobic digestion.
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Zdeb, Magdalena, Marta Bis, and Artur Przywara. "Multi-Criteria Analysis of the Influence of Lignocellulosic Biomass Pretreatment Techniques on Methane Production." Energies 16, no. 1 (January 1, 2023): 468. http://dx.doi.org/10.3390/en16010468.

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Methane from environmentally friendly anaerobic digestion may be an alternative non-renewable source that is depleting. One of the substrates for that process may be lignocellulose-based materials. The article concerns comparing the environmental impact as well as technical and energy indicators of alternative ways of producing methane from the anaerobic digestion of Pennisetum hybrid. Five scenarios were analyzed: methane production from the anaerobic digestion of the raw grass, the grass subjected to alkaline pretreatment (with 2% NaOH solution at two temperatures), and the grass subjected to mechanical pretreatment (ground to obtain particle sizes <0.18 mm and 0.25–0.38 mm). Multi-criteria decision (MCA) analysis was carried out with the use of five indicators, including life cycle assessment results as well as methane production parameters, in order to optimize this sustainable way of bioenergy production. The purpose of this study was to identify the most cost-effective and environmentally friendly method of Pennisetum hybrid pretreatment in order to optimize the methane production process in terms of environmental, technical, and economic aspects. According to the obtained results, it was stated that the most advantageous solution for the majority of the analyzed indicators turned out to be the mechanical pretreatment with grinding the lignocellulosic biomass into a particle size <0.18 mm.
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Manyi-Loh, Christy E., and Ryk Lues. "Anaerobic Digestion of Lignocellulosic Biomass: Substrate Characteristics (Challenge) and Innovation." Fermentation 9, no. 8 (August 13, 2023): 755. http://dx.doi.org/10.3390/fermentation9080755.

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Modern society is characterised by its outstanding capacity to generate waste. Lignocellulosic biomass is most abundant in nature and is biorenewable and contains energy sources formed via biological photosynthesis from the available atmospheric carbon dioxide, water, and sunlight. It is composed of cellulose, hemicellulose, and lignin, constituting a complex polymer. The traditional disposal of these types of waste is associated with several environmental and public health effects; however, they could be harnessed to produce several value-added products and clean energy. Moreover, the increase in population and industrialisation have caused current energy resources to be continuously exploited, resulting in the depletion of global fuel reservoirs. The overexploitation of resources has caused negative environmental effects such as climate change, exacerbating global greenhouse gas emissions. In the quest to meet the world’s future energy needs and adequate management of these types of waste, the anaerobic digestion of lignocellulosic biomass has remained the focus, attracting great interest as a sustainable alternative to fossil carbon resources. However, substrate characteristics offer recalcitrance to the process, which negatively impacts the methane yield. Nevertheless, the biodigestibility of these substrates can be enhanced through chemical, physical, and biological pretreatment methods, leading to improvement in biogas yields. Furthermore, the co-digestion of these substrates with other types and adding specific nutrients as trace elements or inoculum will help to adjust substrate characteristics to a level appropriate for efficient anaerobic digestion and increased biogas yield.
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Matheri, Anthony Njuguna, Freeman Ntuli, Jane Catherine Ngila, Tumisang Seodigeng, Caliphs Zvinowanda, and Cecilia Kinuthia Njenga. "Quantitative characterization of carbonaceous and lignocellulosic biomass for anaerobic digestion." Renewable and Sustainable Energy Reviews 92 (September 2018): 9–16. http://dx.doi.org/10.1016/j.rser.2018.04.070.

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Liew, Lo Niee, Jian Shi, and Yebo Li. "Methane production from solid-state anaerobic digestion of lignocellulosic biomass." Biomass and Bioenergy 46 (November 2012): 125–32. http://dx.doi.org/10.1016/j.biombioe.2012.09.014.

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Wright, Alexander, Andrew Rollinson, Dipti Yadav, Szymon Lisowski, Felipe Iza, Richard Holdich, Tanja Radu, and H. C. Hemaka Bandulasena. "Plasma-assisted pre-treatment of lignocellulosic biomass for anaerobic digestion." Food and Bioproducts Processing 124 (November 2020): 287–95. http://dx.doi.org/10.1016/j.fbp.2020.09.005.

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Zulkifli, Zulfah, Nazaitulshila Rasit, Noor Azrimi Umor, and Shahrul Ismail. "The effect of A. Fumigatus SK1 and trichoderma sp. on the biogas production from cow manure." Malaysian Journal of Fundamental and Applied Sciences 14, no. 3 (September 3, 2018): 353–59. http://dx.doi.org/10.11113/mjfas.v14n3.1066.

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Lignocellulosic material consists of lignin, cellulose and hemicellulose. Converting lignocellulosic biomass such as cow manure (CM) into value-added products provides a potential alternative. Hydrolysis of cellulose and hemicellulose is a limiting step during Anaerobic Digestion (AD) of lignocellulosic biomass. Lignin in lignocellulosic biomass is the barrier for hydrolysis, thus limits the biogas production. In this study, the effect of A.Fumigatus SK1 and Trichoderma sp. on enzymatic pre-treatment of CM was investigated with respect to the biogas production. Three set of anaerobic digestion assays were carried out, with a working volume of 500 mL at 35 ± 2°C and 120 rpm. The first set of fermentation contained untreated CM. The second set of fermentation involved addition of A.Fumigatus SK1, and the last set contained Trichoderma sp. Several analysis were conducted to determine the biomethane potential (BMP), anaerobic biodegradability, reducing sugars concentration and lignin removal of CM before and after pre-treatment. Result showed that, among both evaluated pre-treatment methods, CM treated with Trichoderma sp. gave the highest methane potential with 0.023 LCH4-STP g VS-1 compared to CM treated with A.Fumigatus SK1(0.011 LCH4-STP g VS-1). A good correlation have been found in this study between lignin removal and reducing sugar produced where, the total lignin removal after treated with Trichoderma sp. was 60% followed by 43% after treated with A.Fumigatus SK1.The reducing sugar produced after pre-treated with Trichoderma sp. and A.Fumigatus SK1 was about 9.59 and 4.91 μmol glucose, respectively. These results collectively suggested that CM treated with Trichoderma sp. could be a better pre-treatment method for the higher methane production in anaerobic mono-digestion process.
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Mahdi, Yasmeen Salih, Asem Hassan Mohammed, and Alaa Kareem Mohammed. "Delignification of Date Palm Fronds using Modified Organosolv Technique." Al-Khwarizmi Engineering Journal 13, no. 3 (September 30, 2017): 1–9. http://dx.doi.org/10.22153/kej.2017.07.001.

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Abstract In this study, modified organic solvent (organosolv) method was applied to remove high lignin content in the date palm fronds (type Al-Zahdi) which was taken from the Iraqi gardens. In modified organosolv, lignocellulosic material is fractionated into its constituents (lignin, cellulose and hemicellulose). In this process, solvent (organic)-water is brought into contact with the lignocellulosic biomass at high temperature, using stainless steel reactor (digester). Therefor; most of hemicellulose will remove from the biomass, while the solid residue (mainly cellulose) can be used in various industrial fields. Three variables were studied in this process: temperature, ratio of ethanol to water and digestion time. Statistical experimental design type Central Composite Design (CCD) has been used to find a mathematical relationship between the variables and the remaining lignin percent as dependent variable. The results obtained in this study were represented by a polynomial mathematical equation of the second degree. The results showed that the best digestion time was (80 minutes), which gave the best percent remaining concentration of lignin (3%) at temperature of 185oC and ratio of ethanol: water equal to 50: 50 wt/wt. In order to reduce digesting time, the effect of using different catalysts have been studied such as (NaOH, H2SO4, Ca (OH) 2) at low concentration (0.025, 0.025, 0.05M) respectively. It was found that the best catalyst is sodium hydroxide at concentration (0.025) mol/L which gave the same percent of lignin 3% but with low digestion time about 30 min. Keywords: Biomass pre-treatment, delignification, lignin, organosolv, date palm fronds.
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Stachowiak–Wencek, Agata, Jan Bocianowski, Hanna Waliszewska, Sławomir Borysiak, Bogusława Waliszewska, and Magdalena Zborowska. "Statistical prediction of biogas and methane yields during anaerobic digestion based on the composition of lignocellulosic biomass." BioResources 16, no. 4 (September 7, 2021): 7086–100. http://dx.doi.org/10.15376/biores.16.4.7086-7100.

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In the described study, the relationships between the percentage and structure of selected lignocellulosic components and the efficiency of their anaerobic digestion and the quality of the produced biogas were analyzed. This research included various lignocellulosic raw materials. The biogas efficiency and quality tests were carried out according to DIN standard 38 414-8 (1985) and VDI standard 4630 (2016). Multiple TAPPI standards and the Seifert method were used to determine the chemical composition of the lignocellulose materials. Lignin structure analysis was performed using Fourier transform infrared spectroscopy. Wide-angle X-Ray scattering analysis was used to determine the degree of crystallinity of cellulose. The biogas was positively correlated with C=O and the syringyl to guaiacyl ratio, and negatively correlated with the crystalline structure of cellulose, lignin, cellulose, and extractives. In addition, methane was positively correlated with holocellulose and extractives and negatively correlated with the crystalline structure of cellulose, cellulose, substances soluble in NaOH, and the OH groups. The found independent features accounted for 86.0% of the biogas variability and 68.0% of the methane variability.
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Kucharska, Karolina, Edyta Słupek, Hubert Cieśliński, and Marian Kamiński. "Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes." Chemical Papers 74, no. 4 (October 21, 2019): 1199–209. http://dx.doi.org/10.1007/s11696-019-00960-1.

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Abstract Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purification prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharification conditions and identify the process parameters that should be applied to improve the saccharification efficiency of lignocellulosic biomass, defined as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at different stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharification enzymatic cocktail were identified and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.
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Mirmohamadsadeghi, Safoora, Keikhosro Karimi, Akram Zamani, Hamid Amiri, and Ilona Sárvári Horváth. "Enhanced Solid-State Biogas Production from Lignocellulosic Biomass by Organosolv Pretreatment." BioMed Research International 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/350414.

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Organosolv pretreatment was used to improve solid-state anaerobic digestion (SSAD) for methane production from three different lignocellulosic substrates (hardwood elm, softwood pine, and agricultural waste rice straw). Pretreatments were conducted at 150 and 180°C for 30 and 60 min using 75% ethanol solution as an organic solvent with addition of sulfuric acid as a catalyst. The statistical analyses showed that pretreatment temperature was the significant factor affecting methane production. Optimum temperature was 180°C for elmwood while it was 150°C for both pinewood and rice straw. Maximum methane production was 152.7, 93.7, and 71.4 liter per kg carbohydrates (CH), which showed up to 32, 73, and 84% enhancement for rice straw, elmwood, and pinewood, respectively, compared to those from the untreated substrates. An inverse relationship between the total methane yield and the lignin content of the substrates was observed. Kinetic analysis of the methane production showed that the process followed a first-order model for all untreated and pretreated lignocelluloses.
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Li, Pengfei, Chao He, Chongbo Cheng, Youzhou Jiao, Dekui Shen, and Ran Yu. "Prediction of methane production from co-digestion of lignocellulosic biomass with sludge based on the major compositions of lignocellulosic biomass." Environmental Science and Pollution Research 28, no. 20 (January 21, 2021): 25808–18. http://dx.doi.org/10.1007/s11356-020-12262-1.

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35

Naji, Amar, Sabrina Guérin Rechdaoui, Elise Jabagi, Carlyne Lacroix, Sam Azimi, and Vincent Rocher. "Horse Manure and Lignocellulosic Biomass Characterization as Methane Production Substrates." Fermentation 9, no. 6 (June 19, 2023): 580. http://dx.doi.org/10.3390/fermentation9060580.

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This paper aimed to study the value of horse manure through anaerobic digestion. The study involved characterization of different components of horse waste and the evaluation of their biochemical composition, physicochemical characterization and the influence of the composition of horse waste on biochemical methane potential. More specifically, two bedding mixtures were studied: the first one was composed of wheat straw (WS), wood chips (WC) and horse manure (HM) with a volumetric composition of 85%, 14% and 1%, respectively; and the second one was a mixture of WS and HM with a volumetric composition of 99% and 1%, respectively. The analysis was carried out on the two bedding mixtures and on each substrate separately with 406 samples from May 2017 to October 2019. Biochemical methane potential tests conducted on these samples showed that the composition and structure of the substrate influenced the BMP. WS had the highest mono-digestion methane production with 176.1 NmL·gVS−1. The second bedding mixture (99% WS, 1% HM) showed a production of 189.4 NmL·gVS−1 compared to 127 NmL·gVS−1 by bedding mixture 1 (85% WS, 14% WC, 1% HM). The difference was due to a dilution effect on methane production caused by the presence of WC rich in lignin.
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36

Yang, Liangcheng, Fuqing Xu, Xumeng Ge, and Yebo Li. "Challenges and strategies for solid-state anaerobic digestion of lignocellulosic biomass." Renewable and Sustainable Energy Reviews 44 (April 2015): 824–34. http://dx.doi.org/10.1016/j.rser.2015.01.002.

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37

Gao, Jing, Li Chen, Ke Yuan, Hemao Huang, and Zongcheng Yan. "Ionic liquid pretreatment to enhance the anaerobic digestion of lignocellulosic biomass." Bioresource Technology 150 (December 2013): 352–58. http://dx.doi.org/10.1016/j.biortech.2013.10.026.

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38

Akobi, Chinaza, Hyeongu Yeo, Hisham Hafez, and George Nakhla. "Single-stage and two-stage anaerobic digestion of extruded lignocellulosic biomass." Applied Energy 184 (December 2016): 548–59. http://dx.doi.org/10.1016/j.apenergy.2016.10.039.

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39

Ge, Xumeng, Fuqing Xu, and Yebo Li. "Solid-state anaerobic digestion of lignocellulosic biomass: Recent progress and perspectives." Bioresource Technology 205 (April 2016): 239–49. http://dx.doi.org/10.1016/j.biortech.2016.01.050.

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40

Yang, Liangcheng, David E. Kopsell, Alisha M. Kottke, and Matthew Q. Johnson. "Development of a cartridge design anaerobic digestion system for lignocellulosic biomass." Biosystems Engineering 160 (August 2017): 134–39. http://dx.doi.org/10.1016/j.biosystemseng.2017.05.004.

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Wang, Dou, Fei Shen, Gang Yang, Yanzong Zhang, Shihuai Deng, Jing Zhang, Yongmei Zeng, Tao Luo, and Zili Mei. "Can hydrothermal pretreatment improve anaerobic digestion for biogas from lignocellulosic biomass?" Bioresource Technology 249 (February 2018): 117–24. http://dx.doi.org/10.1016/j.biortech.2017.09.197.

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42

Sawatdeenarunat, Chayanon, Hyungseok Nam, Sushil Adhikari, Shihwu Sung, and Samir Kumar Khanal. "Decentralized biorefinery for lignocellulosic biomass: Integrating anaerobic digestion with thermochemical conversion." Bioresource Technology 250 (February 2018): 140–47. http://dx.doi.org/10.1016/j.biortech.2017.11.020.

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43

Ali, Shehbaz, Tawaf A. Shah, Asifa Afzal, and Romana Tabassum. "Exploring lignocellulosic biomass for bio-methane potential by anaerobic digestion and its economic feasibility." Energy & Environment 29, no. 5 (March 1, 2018): 742–51. http://dx.doi.org/10.1177/0958305x18759009.

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Anaerobic digestion is a process to convert organic biomass into bio-methane. Plenty of produced waste in Pakistan is enough to compensate energy thirst of country and have potential to replace costly fossil fuels. The lignocellulosic biomass such as wheat straw, almond shell, sugarcane bagasse, maize straw and corn cob were subjected to bio-methane potential assay after proximate, ultimate and chemical analysis. These chemical fractions provide better understanding about theoretically predicating bio-methane potentials such as neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose, hemicellulose, carbohydrates, proteins and elemental analysis. Experimental bio-methane potentials were found, 267.74 (wheat straw), 255.32 (almond shell), 222.23 (corn cob), 247.60 (sugar cane bagasse) and 293.12 ml/g (maize straw) volatile solids and was much less than predicted methane potential. The energy content on dry basis and methane potential has been assessed to find economic feasibility of biomass. The biodegradability and methane potential inversely related to the lignin content of biomass. Bioenergy production from biomass is economically favourable. The volatile fatty acids were produced in the percentage of 53–58% acetic acid, 30–35% butyric acids and 6–13% propionic acid and showed same metabolic pathway and types of bacteria involved in digestion.
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44

Marin-Batista, Jose D., Angel F. Mohedano, and Angeles de la Rubia. "Pretreatment of Lignocellulosic Biomass with 1-Ethyl-3-methylimidazolium Acetate for Its Eventual Valorization by Anaerobic Digestion." Resources 10, no. 12 (November 23, 2021): 118. http://dx.doi.org/10.3390/resources10120118.

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This study assessed the breakdown of lignocellulosic biomass (LB) with the ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]) as a pretreatment to increase the methane yield. The pretreatment was conducted for wheat straw (WS), barley straw (BS), and grape stem (GS) at 120 °C for 120 min, using several LB to [Emim][Ac] ratios (1:1, 1:3, and 1:5 w/w). Pretreatment significantly disrupted the lignocellulose matrix of each biomass into soluble sugars. GS showed the highest sugar yield, which was followed by WS, while BS was slightly hydrolyzed (175.3 ± 2.3, 158.2 ± 5.2, and 51.1 ± 3.1 mg glucose g–1 biomass, respectively). Likewise, the pretreatment significantly reduced the cellulose crystallinity index (CrI) of the resulting solid fractions of GS and WS by 15% and 9%, respectively, but slightly affected the CrI of BS (5%). Thus, BMP tests were only carried out for raw and hydrothermally and [Emim][Ac] (1:5) pretreated GS and WS. The untreated GS and WS showed similar methane yields to those achieved for the solid fraction obtained after pretreatment with an LB to [Emim][Ac] ratio of 1:5 (219 ± 10 and 368 ± 1 mL CH4 g–1 VS, respectively). The methane production of the solid plus liquid fraction obtained after IL pretreatment increased by 1.61- and 1.34-fold compared to the raw GS and WS, respectively.
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Ghimire, Nirmal, Rune Bakke, and Wenche Hennie Bergland. "Mesophilic Anaerobic Digestion of Hydrothermally Pretreated Lignocellulosic Biomass (Norway Spruce (Picea abies))." Processes 9, no. 2 (January 20, 2021): 190. http://dx.doi.org/10.3390/pr9020190.

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Hot water extraction (HWE) removes hemicellulose from woody biomass to give improved end products while producing a sugar-rich by-product stream, which requires proper treatment before disposal. Hot water extracted Norway spruce (Picea abies) at two different pretreatment conditions (140 °C for 300 min (H140) and 170 °C for 90 min (H170)) generated hydrolysate as a by-product, which was used in mesophilic anaerobic digestion (AD) as substrate. H140 gave a higher methane yield (210 NmL/g COD—chemical oxygen demand) than H170 (148 NmL/g COD) despite having a lower concentration of sugars, suggesting that different levels of inhibitors (furans and soluble lignin) and recalcitrant compounds (soluble lignin) affected the methane yield significantly. Organic loads (OLs) had a negative effect on the methane yield, as observed during AD of H170, while such an effect was not observed in the case of H140. This suggests that the decrease in methane yield (32%) of H170 compared to H140 is primarily due to inhibitors, while the decrease in methane yield (19%) of H140 compared to the synthetic hydrolysate is primarily due to recalcitrant substances. Therefore, both OL and pretreatment conditions must be considered for efficient anaerobic digestion from hydrolysate for enhanced methane production.
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Ma, Shuaishuai, Hongliang Wang, Jingxue Li, Yu Fu, and Wanbin Zhu. "Methane production performances of different compositions in lignocellulosic biomass through anaerobic digestion." Energy 189 (December 2019): 116190. http://dx.doi.org/10.1016/j.energy.2019.116190.

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Olugbemide, Akinola David, Ana Oberlintner, Uroš Novak, and Blaž Likozar. "Lignocellulosic Corn Stover Biomass Pre-Treatment by Deep Eutectic Solvents (DES) for Biomethane Production Process by Bioresource Anaerobic Digestion." Sustainability 13, no. 19 (September 22, 2021): 10504. http://dx.doi.org/10.3390/su131910504.

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The valorization study of the largely available corn stover waste biomass after pretreatment with deep eutectic solvent (DES) for biomethane production in one-liter glass bioreactors by anaerobic digestion for 21 days was presented. Ammonium thiocyanate and urea deep eutectic solvent pretreatments under different conditions in terms of the components ratio and temperature were examined on corn stover waste biomass. The lignocellulose biomass was characterized in detail for its chemistry and morphology to determine the effect of the pretreatment on the natural biocomposite. Furthermore, the implications on biomethane production through anaerobic digestion with different loadings of corn stover biomass at 35 g/L and 50 g/L were tested. The results showed an increase of 48% for a cumulative biomethane production for a DES-pretreated biomass, using a solid-to-liquid ratio of 1:2 at 100 °C for 60 min, which is a strong indication that DES-pretreatment significantly enhanced biomethane production.
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Phuong Thi Vu, Rameshprabu Ramaraj, Prakash Bhuyar, and Yuwalee Unpaprom. "The possibility of aquatic weeds serving as a source of feedstock for bioethanol production: a review." Maejo International Journal of Energy and Environmental Communication 4, no. 2 (July 15, 2022): 50–63. http://dx.doi.org/10.54279/mijeec.v4i2.248180.

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Anaerobic digestion is recognized as an attractive option for the effective management and treatment of lignocellulosic biomass as well as waste recovery of resources for bioethanol production. Long enough testing has been done on bioethanol production using lignocellulosic biomass. This helps to reduce stress and global energy problems. Global wide has a variety of environmental impacts due to its use of fossil fuels. Bioethanol might be produced in Asian locations from many types of biomasses, including agricultural waste, forest waste, and wood biomass. This would be an environmentally friendly process. Unfortunately, there is very little research into the production of ethanol from rice field weeds. This makes it difficult to develop bioethanol production. This review is aimed at developing bioethanol production and the trend towards organic products that began nowadays. Unwanted weed growth is a major problem in rice cultivation. This review demonstrated the waste-to-energy aspect of the bioethanol production process using two weeds, gooseweed (Sphenoclea Zeylanica), and small-flowered nutsedge (Cyperus difformis).
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Obey Gotore, Vadzanayi Mushayi, and Sawitree Tipnee. "Evaluation of cattail characteristics as an invasive wetland plant and biomass usage management for biogas generation." Maejo International Journal of Energy and Environmental Communication 3, no. 2 (May 25, 2021): 1–6. http://dx.doi.org/10.54279/mijeec.v3i2.245167.

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The fossil fuel-based linear economy has many severe drawbacks, including the need for energy security and the resulting environmental degradation. In a new cycle of the bio-economy that is becoming increasingly important, biomass waste has been used to generate energy while reducing pollution and greenhouse gas emissions. The growth of renewable energy will be substantial in the reduction of greenhouse gas emissions in order to achieve the ambitious goal of becoming carbon neutral by the mid-century. It appears that using anaerobic digestion technology to produce methane-rich biogas from biomass has a great deal of potential in this scenario. The cattail fresh and dry biomass substrate with pig wastes as inoculum was tested for biogas production. Cattail's highly complex lignocellulosic structures make it challenging to decompose as a biogas substrate. Alkaline pretreatment is one of the efficient tools in solubilizing lignin. As a result, chemical pretreatment of biomass (2 % sodium hydroxide) was a unique method for increasing biogas generation by reducing complex polymers of lignocellulosic materials into simpler molecules that microorganisms could digest. The fresh and dry biomass substrate added fermenter was produced with 57% and 60% methane, respectively.
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Stachowiak-Wencek, Agata, Magdalena Zborowska, Hanna Waliszewska, and Bogusława Waliszewska. "Chemical changes in lignocellulosic biomass (corncob) influenced by pretreatment and anaerobic digestion (AD)." BioResources 14, no. 4 (August 21, 2019): 8082–99. http://dx.doi.org/10.15376/biores.14.4.8082-8099.

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Changes in chemical composition and structure of corncob lignocellulosic biomass were investigated relative to pretreatment and anaerobic digestion. The pretreatment involved 1% and 3% sodium hydroxide, 3% and 7% sulphuric acid, as well as medium and high temperature extrusion (in 110 °C and in the range from 140 °C to 160 °C). The chemical components content was studied using a gravimetric method, whereas structure and relations between the carbohydrate and lignin participation were investigated using Fourier transform infrared spectroscopy. It was determined that the chemical treatment, both acidic and alkaline, changed the chemical composition of corncob more significantly than the extrusion. Alkaline pretreatment contributed to significant delignification, while acidic pretreatment reduced the share of hemicelluloses and increased the proportion of lignin, the so-called “pseudolignin”. The composition of corncob (control and after pre-treatment) was changed after anaerobic digestion, i.e., a decreased carbohydrate substance content and a significantly increased lignin content. FTIR analysis showed changes in their structure. Although the control corncob differed from that processed by various pretreatment methods, the chemical composition of the digested pulp obtained from them was similar. The NaOH pretreatment was judged to be the preferred method for delignification of the raw material.
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