Relevant bibliographies by topics / DiaPASEF

Academic literature on the topic 'DiaPASEF'

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Published: 20 July 2024

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Journal articles on the topic "DiaPASEF"

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Arenas-De Larriva, María del Sol, Alejandro Fernández-Vega, Bernabe Jurado-Gamez, and Ignacio Ortea. "diaPASEF Proteomics and Feature Selection for the Description of Sputum Proteome Profiles in a Cohort of Different Subtypes of Lung Cancer Patients and Controls." International Journal of Molecular Sciences 23, no. 15 (August 5, 2022): 8737. http://dx.doi.org/10.3390/ijms23158737.

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The high mortality, the presence of an initial asymptomatic stage and the fact that diagnosis in early stages reduces mortality justify the implementation of screening programs in the populations at risk of lung cancer. It is imperative to develop less aggressive methods that can complement existing diagnosis technologies. In this study, we aimed to identify lung cancer protein biomarkers and pathways affected in sputum samples, using the recently developed diaPASEF mass spectrometry (MS) acquisition mode. The sputum proteome of lung cancer cases and controls was analyzed through nano-HPLC–MS using the diaPASEF mode. For functional analysis, the results from differential expression analysis were further analyzed in the STRING platform, and feature selection was performed using sparse partial least squares discriminant analysis (sPLS-DA). Our results showed an activation of inflammation, with an alteration of pathways and processes related to acute-phase, complement, and immune responses. The resulting sPLS-DA model separated between case and control groups with high levels of sensitivity and specificity. In conclusion, we showed how new-generation proteomics can be used to detect potential biomarkers in sputum samples, and ultimately to discriminate patients from controls and even to help to differentiate between different cancer subtypes.
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Meier, Florian, Andreas-David Brunner, Max Frank, Annie Ha, Isabell Bludau, Eugenia Voytik, Stephanie Kaspar-Schoenefeld, et al. "diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition." Nature Methods 17, no. 12 (November 30, 2020): 1229–36. http://dx.doi.org/10.1038/s41592-020-00998-0.

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Wen, Chengwen, Guohong Gan, Xiao Xu, Guanzhong Lin, Xi Chen, Yaying Wu, Zheni Xu, et al. "Investigation of Effects of the Spectral Library on Analysis of diaPASEF Data." Journal of Proteome Research 21, no. 2 (December 31, 2021): 507–18. http://dx.doi.org/10.1021/acs.jproteome.1c00899.

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Gillespie, D. R., and D. M. J. Quiring. "Effects of photoperiod on induction of diapause in Feltiella acarisuga (Diptera: Cecidomyiidae)." Canadian Entomologist 134, no. 1 (February 2002): 69–75. http://dx.doi.org/10.4039/ent13469-1.

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AbstractLarvae of the predatory gall midge Feltiella acarisuga (Vallot) diapaused as prepupae in tightly woven, brown, silk cells on leaf surfaces. Photoperiod alone, at day lengths from 16 to 8 h did not induce diapause at either 20 or 25 °C. A low incidence of diapause was induced by a combined photoperiod and thermoperiod of an 8-h day at 25 °C and a 16-h night at 15 °C. The incidence of diapause was higher under these conditions if the larvae were fed diapausing spider mites, Tetranychus urticae (Koch) (Acari: Tetranychidae). Because F. acarisuga only diapauses at daylengths equivalent to mid-winter, when its prey, T. urticae, is also in diapause, it can be used as a biological control agent for T. urticae in British Columbia greenhouses throughout most of the growing season.
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Huang, Zelu, Weijia Kong, Bertrand Jernhan Wong, Huanhuan Gao, Tiannan Guo, Xianming Liu, Xiaoxian Du, Limsoon Wong, and Wilson Wen Bin Goh. "Proteomic datasets of HeLa and SiHa cell lines acquired by DDA-PASEF and diaPASEF." Data in Brief 41 (April 2022): 107919. http://dx.doi.org/10.1016/j.dib.2022.107919.

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Jiang, Ting, Yulin Zhu, Yingchuan Peng, Wanna Zhang, and Haijun Xiao. "Universal and differential transcriptional regulatory pathways involved in the preparation of summer and winter diapauses in Pieris melete." Bulletin of Entomological Research 111, no. 3 (February 1, 2021): 371–78. http://dx.doi.org/10.1017/s0007485321000018.

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AbstractMuch progress has been made in understanding the environmental and hormonal systems regulating winter diapause. However, transcriptional regulation of summer diapause is still largely unknown, making it difficult to understand an all-around regulation profile of seasonal adaptation. To bridge this gap, comparison RNA-seq to profile the transcriptome and to examine differential gene expression profiles between non-diapause, summer diapause, and winter diapause groups were performed. A total number of 113 million reads were generated and assembled into 79,117 unigenes, with 37,492 unigenes categorized into 58 functional gene ontology groups, 25 clusters of orthologous group categories, and 256 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG analysis mapped 2108 differentially expressed genes to 48 and 67 pathways for summer and winter diapauses, respectively. Enrichment statistics showed that 11 identical pathways similarly overlapped in the top 20 enriched functional groups both related to summer and winter diapauses. We also identified 35 key candidate genes for universal and differential functions related to summer and winter diapause preparation. Furthermore, we identified some genes involved in the signaling and metabolic pathways that may be the key drivers to integrate environmental signals into the summer and winter diapause preparation. The current study provided valuable insights into global molecular mechanisms underpinning diapause preparation.
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Pokhrel, Narayan, Olga Genin, Dalit Sela-Donenfeld, and Yuval Cinnamon. "HREM, RNAseq and Cell Cycle Analyses Reveal the Role of the G2/M-Regulatory Protein, WEE1, on the Survivability of Chicken Embryos during Diapause." Biomedicines 10, no. 4 (March 27, 2022): 779. http://dx.doi.org/10.3390/biomedicines10040779.

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Avian blastoderm can enter into diapause when kept at low temperatures and successfully resume development (SRD) when re-incubated in body temperature. These abilities, which are largely affected by the temperature and duration of the diapause, are poorly understood at the cellular and molecular level. To determine how temperature affects embryonic morphology during diapause, high-resolution episcopic microscopy (HREM) analysis was utilized. While blastoderms diapausing at 12 °C for 28 days presented typical cytoarchitecture, similar to non-diapaused embryos, at 18 °C, much thicker blastoderms with higher cell number were observed. RNAseq was conducted to discover the genes underlying these phenotypes, revealing differentially expressed cell cycle regulatory genes. Among them, WEE1, a negative regulator of G2/M transition, was highly expressed at 12 °C compared to 18 °C. This finding suggested that cells at 12 °C are arrested at the G2/M phase, as supported by bromodeoxyuridine incorporation (BrdU) assay and phospho-histone H3 (pH 3) immunostaining. Inhibition of WEE1 during diapause at 12 °C resulted in cell cycle progression beyond the G2/M and augmented tissue volume, resembling the morphology of 18 °C-diapaused embryos. These findings suggest that diapause at low temperatures leads to WEE1 upregulation, which arrests the cell cycle at the G2/M phase, promoting the perseverance of embryonic cytoarchitecture and future SRD. In contrast, WEE1 is not upregulated during diapause at higher temperature, leading to continuous proliferation and maladaptive morphology associated with poor survivability. Combining HREM-based analysis with RNAseq and molecular manipulations, we present a novel mechanism that regulates the ability of diapaused avian embryos to maintain their cytoarchitecture via cell cycle arrest, which enables their SRD.
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Galka, Brian E., and Reinhart A. Brust. "The effect of temperature and photoperiod on the induction of larval diapause in the mosquito Aedes togoi (Theobald) (Diptera: Culicidae)." Canadian Journal of Zoology 65, no. 9 (September 1, 1987): 2262–65. http://dx.doi.org/10.1139/z87-341.

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Aedes togoi (Theobald) from Vancouver, B.C., may enter diapause in either the egg or larval stage. Larval diapause occurred in the fourth instar, when immatures were maintained at 16 °C or less, at photoperiods shorter than 12 h light/day. The critical photoperiod was calculated as 10.9L:13.1D at 16 °C. Larvae that failed to pupate after 40 days following hatching were recorded as being in diapause. At light periods greater than 12 h at 16 °C, there was no larval diapause and, at 14 °C, 100% of the larvae diapaused at 10L:14D. Temperatures of 18 °C or higher, at 10L:14D, prevented larval diapause.
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Chalar, Cora, Graciela Clivio, Jimena Montagne, Alicia Costábile, Analía Lima, Nicolás G. Papa, Nibia Berois, and María José Arezo. "Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III." PLOS ONE 16, no. 6 (June 4, 2021): e0251820. http://dx.doi.org/10.1371/journal.pone.0251820.

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Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a “shotgun” proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. Data are available via ProteomeXchange with identifier PXD025196. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapause-associated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.
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Hansen, J. D., M. A. Watkins, M. L. Heidt, and P. A. Anderson. "Cold Storage to Control Codling Moth Larvae in Fresh Apples." HortTechnology 17, no. 2 (April 2007): 195–98. http://dx.doi.org/10.21273/horttech.17.2.195.

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Codling moth [Cydia pomonella (Lepidoptera: Tortricidae)], found in exported apples (Malus sylvestris), can disrupt international markets. Cold storage at 1.1 °C was examined for possible control of three physiological larval states in ‘Fuji’ apples: diapausing (overwintering), diapause-destined, and nondiapausing. All nondiapausing larvae were dead within 12 weeks, diapaused-destined larvae were controlled by the seventh week, yet more than half of the original populations of diapausing larvae were still alive after 11 weeks. Because the diapaused-destined larvae were younger than the nondiapausing larvae, they may have been more susceptible to cold. Because larvae normally diapause outside the fruit, cold storage would not be applicable for controlling larvae in this state.
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Dissertations / Theses on the topic "DiaPASEF"

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Rijal, Jeewan Babu. "Development and optimization of high-performing quantitative proteomics methods : application to the discovery of biomarkers for the early diagnosis of multiple sclerosis." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAF015.

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De récentes et importantes innovations instrumentales, analytiques et bioinformatiques permettent aujourd’hui à l’analyse protéomique par spectrométrie de masse (MS) d'atteindre des niveaux de sensibilité et de couverture du protéome jamais atteints auparavant, ce qui laisse entrevoir de nouveaux espoirs pour la découverte de biomarqueurs de pathologies.Ce travail de doctorat se concentre sur le développement et l'optimisation de méthodes enprotéomique, incluant la préparation des échantillons et leur automatisation, la mise au point de méthodes d'acquisition MS utilisant des modes diaPASEF sur un instrument timsTOF de dernière génération et enfin l'évaluation de stratégies d'interprétation des données adaptées. Les méthodes optimisées ont ensuite été appliquées et ont permis d’identifier 46 candidats biomarqueurs robustes pour la sclérose en plaques, au travers de divers types d'échantillons sur un modèle murin et des fluides corporels humains. Huit de ces biomarqueurs ont été validés avec succès par ELISA, soulignant la pertinence des méthodes développées
Recent major instrumental, analytical and computational innovations are enabling massspectrometry-based proteomics to achieve previously unmet levels of sensitivity and proteome coverage and thus hold new promises for biomarker discovery studies.This PhD work focuses on proteomics method developments and optimizations including the sample preparation and its automation, the fine tuning of MS acquisition methods using diaPASEF on a latest generation timsTOF instrument and the benchmarking of adapted data interpretation strategies. Optimized workflows were then applied to identify 46 robust biomarker candidates for Multiple Sclerosis, detected across various sample types on a mouse model and human body fluids. Eight of these biomarkers were successfully validated by orthogonal ELISA, underlining the effectiveness of our optimized MS workflows
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Zola, Sheri. "Molecular events associated with mosquito diapause." Diss., Online access via UMI:, 2006.

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Flannagan, Ronald D. "Sarcophaga crassipalpis pupal diapause: A molecular approach /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487946103566816.

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Schiesari, Luca. "The dilp2/5 genes control diapause inducibility." Doctoral thesis, Università degli studi di Padova, 2013. http://hdl.handle.net/11577/3425834.

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Many holometabolous insects hibernate by triggering diapause, an “actively-induced” dormancy that blocks developmental functions. Yet, the nature of signals enhancing the plasticity of developmental system and underlying diapause inducibility is still elusive. We show that the “Insulin/IGF” dilp2/5 genes, encoding for developmental hormones, antagonize diapause switch in D. melanogaster and their modulation is pivotal in sensitizing the developmental system to environmental perturbations. Functional impairment of dilp2/5 signaling results in the appearance, or inhibition, of the inducible diapause polyphenism, revealing that they are at the core of the gene network regulating diapause inducibility, beyond the control of developmental time. DILP2/5, as dispensable developmental hormones, cover a latent and hidden plasticity of development, underlying the evolution of an inducible diapause polyphenism through genetic accommodation. Such hormonal mechanism might be the putative target to bioengineer diapause inducibility.
Molti insetti olometaboli innescano la diapausa, una dormienza attivamente indotta che blocca lo sviluppo al fine di ibernare. La natura dei segnali che aumentano la plasticità del sistema di sviluppo e che sottendono l’inducibilità della diapausa rimane largamente sconosciuta. Qui, noi riportiamo che dilp2/5, due geni “Insulin/IGF” simili codificanti per ormoni di crescita, reprimono l’induzione della diapausa in D. melanogaster e che la loro modulazione è cruciale nel sensibilizzare il sistema di sviluppo alle perturbazioni ambientali. Modificazioni funzionali di dilp2/5 provocano l’induzione, o l’inibizione, del polifenismo reversibile di diapausa, rivelando che, oltre il loro ruolo nella modulazione del tasso di sviluppo, questi geni sono al cuore del network genico che regola la dormienza. DILP2/5, come ormoni di crescita dispensabili per il normale sviluppo, mascherano una latente plasticità di sviluppo e la loro modificazione può provocare l’evoluzione della diapausa attraverso accomodazione genica. Questo controllo ormonale potrebbe costituire un promettente bersaglio per un’ingegnerizzazione genetica dell’inducibilità della diapausa.
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Young, Curtis Eugene. "Diapause amd nutritional influences on reproduction and diapause induction in the elm leaf beetle, Xanthogaleruca luteola (Müller) (Coleoptera: chrysomelidae) /." The Ohio State University, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487693923198412.

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Larrère, Myriam. "Physiologie de la diapause imaginale du bourdon, Bombus terrestris : Essais d'obtention de colonies actives en toutes saisons." Bordeaux 1, 1993. http://www.theses.fr/1993BOR10535.

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Nos efforts pour maitriser le cycle biologique du bourdon, bombus terrestris, nous ont conduit a etudier les mecanismes physiologiques impliques dans le controle de la diapause et de la reproduction. A l'issue de ce travail, nous sommes capables de proposer des methodes susceptibles d'interrompre a tout moment le processus de diapause. Parmi ces methodes, la narcose au co#2 s'avere particulierement efficace. La rupture de diapause s'accompagne de la reactivation des pericaryons neurosecreteurs cerebraux mais pas de celle des corpora allata. Nos resultats remettent en cause le role de l'hormone juvenile dans le controle de la diapause imaginale du bourdon. La reproduction chez cet insecte social est largement tributaire du statut social de la femelle. Nous montrons que la dominance associee a une production de jh elevee permet une maturation ovarienne rapide et le succes de la ponte
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Smith, Kent James. "Enhanced Cold Tolerance of Diapause-Destined vs Non-Diapause-Destined Larval Stages of the Flesh Fly, Sarcophaga Crassipalpis(Diptera: Sarcophagidae)." Oxford, Ohio : Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1172780125.

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Williams, Karen Delores. "A genetic analysis of diapause in Drosophila melanogaster." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ66369.pdf.

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Lambert, Roger Thomas. "Embryonic diapause in European roe deer (Capreolus capreolus)." Thesis, University of Aberdeen, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394554.

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The total gestation period in the roe deer is approximately 290 days (10 months). When the blastocyst reaches the 20-30 stage it enters a 5-month period of diapause. During the last six weeks of diapause the blastocyst undergoes mitosis and reactivates in late December or early January at the 100-cell stage. The conceptus undergoes a period of rapid growth, which lasts between 7 and 25 days, before attachment to the endometrium and normal fetal growth. By studying the period between late diapause and early fetal development, it was possible to monitor changes in the reproductively important hormones, progesterone, oestradiol-17β, prolactin and pregnancy-associated glycoproteins as well as the secretion of important signalling proteins by the conceptus and uterus. In addition, investigations were undertaken to determine whether a regimen of long day photoperiod manipulation would effect the duration of diapause. Both peripheral concentrations and luteal release of progesterone remained consistent throughout diapause, reactivation and implantation (peripheral progesterone: 3.82 ± 1.97 ng ml-1; luteal progesterone: 6.72 ± 0.81 ng mg-1 protein). Oestradiol concentrations remained low during diapause (1.07 ± 0.4 pg ml-1) and expansion (1.2 ± 0.4 pg ml-1) but increased 30 times at trophoblast elongation (49.17 ± 0.37 pg ml-1) but increased 30 times at trophoblast elongation (49.17 ± 0.37 pg ml-1). Both prolactin and pregnancy-associated glycoproteins remained at basal concentrations (4.69 ± 0.86 pg ml-1 and <4.5 ng-ml-1 respectively) and increased after implantation (12.34 ± 2.71 ng ml-1 and 7.74 ± 0.47 ng ml-1 respectively). Incorporation of a radiolabel into conceptus secretory proteins increased four times at expansion compared with diapause, whereas incorporation into endometrial secretions remained constant. At elongation, incorporation into endometrial secretions increased two times and conceptus secretions increased 32 times. Endometrial secretions remained at these levels until implantation when they decreased linearly as fetal development progressed. Two-dimensional electrophoresis and fluorography showed that the profile of endometrial secretory proteins was constant until implantation when qualitative changes were evident.
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Martin, Anduaga Ane. "Diapause and the circadian clock in Drosophila melanogaster." Thesis, University of Leicester, 2018. http://hdl.handle.net/2381/42771.

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As a strategy to survive to the upcoming winter, many insects enter diapause (a typical overwintering response that results on their developmental arrest). Drosophila melanogaster undergoes an adult or reproductive diapause that can be easily spotted by looking at the stage of development of the females’ ovaries. The possibility of the circadian clock influencing this phenotype was proposed to explain photoperiodic differences in induction levels. Nevertheless, to the date the debate is still on. In this thesis, I looked at several canonical clock mutants and assessed their impact on diapause, finding that 1) depending on the temperature in which they were reared the effects on the adult flies varied enormously 2) most of the clock mutants gave a strong effect in one or other growing conditions. In particular, Pdf0 and ClkJrk mutants behave in completely opposite ways. A second part of the project consisted on looking at the effects of period temperature-sensitive splicing in diapause. Using splicing locked transgenic flies provided by Isaac Edery, I found that expression of the summer isoform impaired the ability of the flies to undergo diapause. Hence, I cloned the different splicing variants into a pUAST vector and generated UAS lines to perform a neuroanatomical dissection of the phenotype. Also, related with the previous project, I decided to look if any miRNA could be regulating diapause by affecting any of the splicing variants. I found several possible miRNAs that could target the summer (intron-containing) non-splicing isoform. I found that one particular, miRNA-276b, was having a huge effect on diapause. Using a sponge particularly against this miRNA (which would result in its downregulation) diapause levels halved compared to all the controls that were performed in parallel.
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Books on the topic "DiaPASEF"

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Papatheodōrou, Vasilēs. Stē diapasōn. Athēna: Ekdoseis Kastaniōtē, 2009.

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Fornes, Antoni. Diapasó amerat. València: E. Climent, 1986.

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Sechin, Anne. Diapason: Roman. Saint-Boniface, Man: Éditions du Blé, 2009.

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Zwang, Gérard. Le diapason. Montpellier, France: Sauramps, 1998.

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Carvajal, Antonio. Diapasón de Epicuro. Sevilla: Fundación El Monte, 2004.

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Carvajal, Antonio. Diapasón de Epicuro. Sevilla: Fundación El Monte, 2004.

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Pyee, Doris. Le diapason 1850-1852. Baltimore, Md: RIPM, 2010.

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Alekseev, Victor R., and Geoffrey Fryer, eds. Diapause in the Crustacea. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1667-8.

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Outram, Richard Daley. Diapason in thimble theatre. [Toronto: Gauntlet Press?, 1997.

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Workshop on Diapause in Aquatic Invertebrates (2003 Pallanza, Italy). Diapause in aquatic invertebrates. Edited by Alekseev V. R and Istituto per lo Studio degli Ecosistemi. Verbania Pallanza, Italy: Istituto per lo Studio degli Ecosistemi, 2004.

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Book chapters on the topic "DiaPASEF"

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Raabe, Marie. "Diapause." In Recent Developments in Insect Neurohormones, 169–80. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0805-8_5.

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Cumming, Jeffrey M., Bradley J. Sinclair, Charles A. Triplehorn, Yousif Aldryhim, Eduardo Galante, Ma Angeles Marcos-Garcia, Malcolm Edmunds, et al. "Diapause." In Encyclopedia of Entomology, 1206–10. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_904.

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Rabinovich, Silvana, and Jane Brodie. "Diapason." In Notes for a Decolonial Political Theology, 1–16. London: Routledge, 2023. http://dx.doi.org/10.4324/9781032679051-1.

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Heppner, John B., John B. Heppner, Minos E. Tzanakakis, Minos E. Tzanakakis, Minos E. Tzanakakis, Pauline O. Lawrence, John L. Capinera, et al. "Obligatory Diapause." In Encyclopedia of Entomology, 2661. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_1809.

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Hodek, Ivo. "Diapause/Dormancy." In Ecology and Behaviour of the Ladybird Beetles (Coccinellidae), 275–342. Chichester, UK: John Wiley & Sons, Ltd, 2012. http://dx.doi.org/10.1002/9781118223208.ch6.

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Krafsur, E. S., R. D. Moon, R. Albajes, O. Alomar, Elisabetta Chiappini, John Huber, John L. Capinera, et al. "Facultative Diapause." In Encyclopedia of Entomology, 1400. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_3739.

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Peck, Stewart B., Carol C. Mapes, Netta Dorchin, John B. Heppner, Eileen A. Buss, Gustavo Moya-Raygoza, Marjorie A. Hoy, et al. "Larval Diapause." In Encyclopedia of Entomology, 1643–48. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_5037.

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Cumming, Jeffrey M., Bradley J. Sinclair, Charles A. Triplehorn, Yousif Aldryhim, Eduardo Galante, Ma Angeles Marcos-Garcia, Malcolm Edmunds, et al. "Diapause Hormone." In Encyclopedia of Entomology, 1210. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_905.

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Alekseev, Victor R., Oscar Ravera, and Bart T. de Stasio. "Introduction to Diapause." In Diapause in Aquatic Invertebrates Theory and Human Use, 3–10. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-5680-2_1.

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Fryer, Geoffrey. "Diapause, a potent force in the evolution of freshwater crustaceans." In Diapause in the Crustacea, 1–14. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1667-8_1.

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Conference papers on the topic "DiaPASEF"

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Yakimova, M. E., V. V. Martemyanov, and N. I. Ershov. "STUDYING THE FEATURES OF ONE- AND TWO-YEAR DEVELOPMENT CYCLES OF DENDROLIMUS SIBIRICUS USING RNA-SEQ DATA." In OpenBio-2023. ИПЦ НГУ, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-43.

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Dendrolimus sibiricus (Siberian silkworm) is the most important pest of the boreal forests of Asia. In the development cycle, this species has a facultative summer diapause, which complicates the population size prediction. This phenomenon has no genetic explanation to date. In this work we have compared differential expression of genes and transcripts for two types of development of the Siberian silkworm based on RNA-seq data.
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Ragland, Gregory. "The (non)conserved diapause transcriptome: What we have learned from molecular natural history of the diapause responses across insect species." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.92818.

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Rice, Marlin E. "Corn Rootworm Management- Reduced Insecticide Rates and Extended Diapause." In Proceedings of the First Annual Crop Production and Protection Conference. Iowa State University, Digital Press, 1991. http://dx.doi.org/10.31274/icm-180809-364.

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Myers, Scott W. "How fumigation efficacy relates to diapause, dose, and temperature." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.91843.

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Bradshaw, William E. "Genomic diversity in the maintenance and termination of diapause." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.91997.

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Sim, Cheolho. "Hormonal and molecular regulation of diapause in the mosquitoCulex pipiens." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.93863.

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Dolezel, David. "Regulation of reproductive diapause in the linden bug,Pyrrhocoris apterus." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94426.

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Hayward, Scott A. L. "Is winter coming? How climate warming disrupts the diapause response." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94937.

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Gotthard, Karl. "The evolution of diapause and alternative developmental pathways in temperate butterflies." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.91999.

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Spacht, Drew E. "The role of PEPCK in insect diapause, development, and stress response." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.113682.

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Reports on the topic "DiaPASEF"

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Logan, Jesse A., Thomas M. ODell, David R. Gray, and [Editors]. Diapause and gypsy moth management: status, applications, and research; 1991 October 2-3; Blacksburg, VA. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experimental Station, 1994. http://dx.doi.org/10.2737/ne-gtr-193.

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Woll, Steven. Insulin-like Growth Factor Pathway Described in Austrofundulus limnaeus Diapause and Escape Embryos. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3198.

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Logan, Jesse A., Thomas M. ODell, David R. Gray, and [Editors]. Diapause and gypsy moth management: status, applications, and research; 1991 October 2-3; Blacksburg, VA. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experimental Station, 1994. http://dx.doi.org/10.2737/ne-gtr-193.

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Altstein, Miriam, and Ronald J. Nachman. Rational Design of Insect Control Agent Prototypes Based on Pyrokinin/PBAN Neuropeptide Antagonists. United States Department of Agriculture, August 2013. http://dx.doi.org/10.32747/2013.7593398.bard.

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The general objective of this study was to develop rationally designed mimetic antagonists (and agonists) of the PK/PBAN Np class with enhanced bio-stability and bioavailability as prototypes for effective and environmentally friendly pest insect management agents. The PK/PBAN family is a multifunctional group of Nps that mediates key functions in insects (sex pheromone biosynthesis, cuticular melanization, myotropic activity, diapause and pupal development) and is, therefore, of high scientific and applied interest. The objectives of the current study were: (i) to identify an antagonist biophores (ii) to develop an arsenal of amphiphilic topically active PK/PBAN antagonists with an array of different time-release profiles based on the previously developed prototype analog; (iii) to develop rationally designed non-peptide SMLs based on the antagonist biophore determined in (i) and evaluate them in cloned receptor microplate binding assays and by pheromonotropic, melanotropic and pupariation in vivo assays. (iv) to clone PK/PBAN receptors (PK/PBAN-Rs) for further understanding of receptor-ligand interactions; (v) to develop microplate binding assays for screening the above SMLs. In the course of the granting period A series of amphiphilic PK/PBAN analogs based on a linear lead antagonist from the previous BARD grant was synthesized that incorporated a diverse array of hydrophobic groups (HR-Suc-A[dF]PRLa). Others were synthesized via the attachment of polyethylene glycol (PEG) polymers. A hydrophobic, biostablePK/PBAN/DH analog DH-2Abf-K prevented the onset of the protective state of diapause in H. zea pupae [EC50=7 pmol/larva] following injection into the preceding larval stage. It effectively induces the crop pest to commit a form of ‘ecological suicide’. Evaluation of a set of amphiphilic PK analogs with a diverse array of hydrophobic groups of the formula HR-Suc-FTPRLa led to the identification of analog T-63 (HR=Decyl) that increased the extent of diapause termination by a factor of 70% when applied topically to newly emerged pupae. Another biostablePK analog PK-Oic-1 featured anti-feedant and aphicidal properties that matched the potency of some commercial aphicides. Native PK showed no significant activity. The aphicidal effects were blocked by a new PEGylated PK antagonist analog PK-dF-PEG4, suggesting that the activity is mediated by a PK/PBAN receptor and therefore indicative of a novel and selective mode-of-action. Using a novel transPro mimetic motif (dihydroimidazole; ‘Jones’) developed in previous BARD-sponsored work, the first antagonist for the diapause hormone (DH), DH-Jo, was developed and shown to block over 50% of H. zea pupal diapause termination activity of native DH. This novel antagonist development strategy may be applicable to other invertebrate and vertebrate hormones that feature a transPro in the active core. The research identifies a critical component of the antagonist biophore for this PK/PBAN receptor subtype, i.e. a trans-oriented Pro. Additional work led to the molecular cloning and functional characterization of the DH receptor from H. zea, allowing for the discovery of three other DH antagonist analogs: Drosophila ETH, a β-AA analog, and a dF analog. The receptor experiments identified an agonist (DH-2Abf-dA) with a maximal response greater than native DH. ‘Deconvolution’ of a rationally-designed nonpeptide heterocyclic combinatorial library with a cyclic bis-guanidino (BG) scaffold led to discovery of several members that elicited activity in a pupariation acceleration assay, and one that also showed activity in an H. zea diapause termination assay, eliciting a maximal response of 90%. Molecular cloning and functional characterization of a CAP2b antidiuretic receptor from the kissing bug (R. prolixus) as well as the first CAP2b and PK receptors from a tick was also achieved. Notably, the PK/PBAN-like receptor from the cattle fever tick is unique among known PK/PBAN and CAP2b receptors in that it can interact with both ligand types, providing further evidence for an evolutionary relationship between these two NP families. In the course of the granting period we also managed to clone the PK/PBAN-R of H. peltigera, to express it and the S. littoralis-R Sf-9 cells and to evaluate their interaction with a variety of PK/PBAN ligands. In addition, three functional microplate assays in a HTS format have been developed: a cell-membrane competitive ligand binding assay; a Ca flux assay and a whole cell cAMP ELISA. The Ca flux assay has been used for receptor characterization due to its extremely high sensitivity. Computer homology studies were carried out to predict both receptor’s SAR and based on this analysis 8 mutants have been generated. The bioavailability of small linear antagonistic peptides has been evaluated and was found to be highly effective as sex pheromone biosynthesis inhibitors. The activity of 11 new amphiphilic analogs has also been evaluated. Unfortunately, due to a problem with the Heliothis moth colony we were unable to select those with pheromonotropic antagonistic activity and further check their bioavailability. Six peptides exhibited some melanotropic antagonistic activity but due to the low inhibitory effect the peptides were not further tested for bioavailability in S. littoralis larvae. Despite the fact that no new antagonistic peptides were discovered in the course of this granting period the results contribute to a better understanding of the interaction of the PK/PBAN family of Nps with their receptors, provided several HT assays for screening of libraries of various origin for presence of PK/PBAN-Ragonists and antagonists and provided important practical information for the further design of new, peptide-based insecticide prototypes aimed at the disruption of key neuroendocrine physiological functions in pest insects.
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Audsley, Neil, Gonzalo Avila, Claudio Ioratti, Valerie Caron, Chiara Ferracini, Tibor Bukovinszki, Marc Kenis, et al. False Codling Moth, Thaumatotibia leucotreta (Meyrick). Euphresco, 2023. http://dx.doi.org/10.1079/20240228687.

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The false codling moth (Thaumatotibia leucotreta) is a major polyphagous pest native to sub-Saharan Africa, affecting over 50 crop species, including citrus, stone fruits, nuts, arable crops, grapevines and vegetables. Larval feeding causes significant damage to fruits, leading to premature ripening and yield losses. The pest, present year-round due to its lack of diapause, is found in African countries and Israel and can spread through trade. Strict phytosanitary measures are required to prevent its spread, impacting trade markets. There are no classical biological control programs for this pest. Promising biological control agents include the egg parasitoid Trichogrammatoidea cryptophlebiae and the larval parasitoid Agathis bishop, both native to southern Africa. Additionally, the entomopathogenic virus Cryptophlebia leucotreta granulovirus (CrleGV) has been used against it in citrus.
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Rafaeli, Ada, and Russell Jurenka. Molecular Characterization of PBAN G-protein Coupled Receptors in Moth Pest Species: Design of Antagonists. United States Department of Agriculture, December 2012. http://dx.doi.org/10.32747/2012.7593390.bard.

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The proposed research was directed at determining the activation/binding domains and gene regulation of the PBAN-R’s thereby providing information for the design and screening of potential PBAN-R-blockers and to indicate possible ways of preventing the process from proceeding to its completion. Our specific aims included: (1) The identification of the PBAN-R binding domain by a combination of: (a) in silico modeling studies for identifying specific amino-acid side chains that are likely to be involved in binding PBAN with the receptor and; (b) bioassays to verify the modeling studies using mutant receptors, cell lines and pheromone glands (at tissue and organism levels) against selected, designed compounds to confirm if compounds are agonists or antagonists. (2) The elucidation ofthemolecular regulationmechanisms of PBAN-R by:(a) age-dependence of gene expression; (b) the effect of hormones and; (c) PBAN-R characterization in male hair-pencil complexes. Background to the topic Insects have several closely related G protein-coupled receptors (GPCRs) belonging to the pyrokinin/PBAN family, one with the ligand pheromone biosynthesis activating neuropeptide or pyrokinin-2 and another with diapause hormone or pyrokinin-1 as a ligand. We were unable to identify the diapause hormone receptor from Helicoverpa zea despite considerable effort. A third, related receptor is activated by a product of the capa gene, periviscerokinins. The pyrokinin/PBAN family of GPCRs and their ligands has been identified in various insects, such as Drosophila, several moth species, mosquitoes, Triboliumcastaneum, Apis mellifera, Nasoniavitripennis, and Acyrthosiphon pisum. Physiological functions of pyrokinin peptides include muscle contraction, whereas PBAN regulates pheromone production in moths plus other functions indicating the pleiotropic nature of these ligands. Based on the alignment of annotated genomic sequences, the primary and secondary structures of the pyrokinin/PBAN family of receptors have similarity with the corresponding structures of the capa or periviscerokinin receptors of insects and the neuromedin U receptors found in vertebrates. Major conclusions, solutions, achievements Evolutionary trace analysisof receptor extracellular domains exhibited several class-specific amino acid residues, which could indicate putative domains for activation of these receptors by ligand recognition and binding. Through site-directed point mutations, the 3rd extracellular domain of PBAN-R was shown to be critical for ligand selection. We identified three receptors that belong to the PBAN family of GPCRs and a partial sequence for the periviscerokinin receptor from the European corn borer, Ostrinianubilalis. Functional expression studies confirmed that only the C-variant of the PBAN-R is active. We identified a non-peptide agonist that will activate the PBAN-receptor from H. zea. We determined that there is transcriptional control of the PBAN-R in two moth species during the development of the pupa to adult, and we demonstrated that this transcriptional regulation is independent of juvenile hormone biosynthesis. This transcriptional control also occurs in male hair-pencil gland complexes of both moth species indicating a regulatory role for PBAN in males. Ultimate confirmation for PBAN's function in the male tissue was revealed through knockdown of the PBAN-R using RNAi-mediated gene-silencing. Implications, both scientific and agricultural The identification of a non-peptide agonist can be exploited in the future for the design of additional compounds that will activate the receptor and to elucidate the binding properties of this receptor. The increase in expression levels of the PBAN-R transcript was delineated to occur at a critical period of 5 hours post-eclosion and its regulation can now be studied. The mysterious role of PBAN in the males was elucidated by using a combination of physiological, biochemical and molecular genetics techniques.
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Horton, David R., Victoria Soroker, Peter Landolt, and Jocelyn Millar. Optimization and field-testing of synthetic sex attractants for two psyllid pests of pears (Hemiptera: Psyllidae). United States Department of Agriculture, October 2011. http://dx.doi.org/10.32747/2011.7594407.bard.

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OBJECTIVES OVERVIEW Improve field activity of pheromone Evaluation of the seasonality of psylla reproduction Evaluate the seasonality of female attractiveness (pheromone production) Evaluate seasonality of male response to synthetic pheromone components Develop practical use for pheromone Test psylla trapping by synthetic pheromone impregnated in rubber septa (provided by J. Millar, cooperator in USA). Test a sprayable formulation of pheromone developed by D. Horton (USA cooperator) SIGNIFICANT FINDINGS No consistent seasonality in female attractiveness was observed. Female attractiveness was observed in winter forms in the end December and in May-June in summer forms. C. bidenshas a very distinct but short period of low mating rate, that is associated with low ovarian development. This short period of low ovarian development and low mating rate cannot be considered as a reproductive diapause. Ovarian development is inhibited by short day photoperiod especially in presence of low temperatures. Male attraction to individual synthetic putative pheromone components is not strong and fluctuates seasonally. While 7MeC27was attractive to males of the winter form, it was not attractive to the summer forms in the beginning of the summer. These data are in accordance with differences in cuticular profiles of winter and summer attractive females vs non attractive ones. Cuticular profile of females shows significant seasonality and difference according to their attractiveness to males especially in summer time. Differences between attractive and non-attractive females is based on several compounds . Female attraciveness in summer and winter is apparently based on a different combination of chemical cues, but 7meC27 is one of the common components to both mixtures. These two last findings are likely to be the main reason for our failure to have consistent success in trapping C. bidensin the field. It seems that a sprayable formulation of pheromone on the pear is better way of pheromone application to attract psyllid then the impregnated rubber septa, but additional trials are needed.
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Rafaeli, Ada, Russell Jurenka, and Chris Sander. Molecular characterisation of PBAN-receptors: a basis for the development and screening of antagonists against Pheromone biosynthesis in moth pest species. United States Department of Agriculture, January 2008. http://dx.doi.org/10.32747/2008.7695862.bard.

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The original objectives of the approved proposal included: (a) The determination of species- and tissue-specificity of the PBAN-R; (b) the elucidation of the role of juvenile hormone in gene regulation of the PBAN-R; (c) the identificationof the ligand binding domains in the PBAN-R and (d) the development of efficient screening assays in order to screen potential antagonists that will block the PBAN-R. Background to the topic: Moths constitute one of the major groups of pest insects in agriculture and their reproductive behavior is dependent on chemical communication. Sex-pheromone blends are utilised by a variety of moth species to attract conspecific mates. In most of the moth species sex-pheromone biosynthesis is under circadian control by the neurohormone, PBAN (pheromone-biosynthesis-activating neuropeptide). In order to devise ideal strategies for mating disruption/prevention, we proposed to study the interactions between PBAN and its membrane-bound receptor in order to devise potential antagonists. Major conclusions: Within the framework of the planned objectives we have confirmed the similarities between the two Helicoverpa species: armigera and zea. Receptor sequences of the two Helicoverpa spp. are 98% identical with most changes taking place in the C-terminal. Our findings indicate that PBAN or PBAN-like receptors are also present in the neural tissues and may represent a neurotransmitter-like function for PBAN-like peptides. Surprisingly the gene encoding the PBAN-receptor was also present in the male homologous tissue, but it is absent at the protein level. The presence of the receptor (at the gene- and protein-levels), and the subsequent pheromonotropic activity are age-dependent and up-regulated by Juvenile Hormone in pharate females but down-regulated by Juvenile Hormone in adult females. Lower levels of pheromonotropic activity were observed when challenged with pyrokinin-like peptides than with HezPBAN as ligand. A model of the 3D structure of the receptor was created using the X-ray structure of rhodopsin as a template after sequence alignment of the HezPBAN-R with several other GPCRs and computer simulated docking with the model predicted putative binding sites. Using in silico mutagenesis the predicted docking model was validated with experimental data obtained from expressed chimera receptors in Sf9 cells created by exchanging between the three extracellular loops of the HezPBAN-R and the Drosophila Pyrokinin-R (CG9918). The chimera receptors also indicated that the 3ʳᵈ extracellular loop is important for recognition of PBAN or Diapause hormone ligands. Implications: The project has successfully completed all the objectives and we are now in a position to be able to design and screen potential antagonists for pheromone production. The successful docking simulation-experiments encourage the use of in silico experiments for initial (high-throughput) screening of potential antagonists. However, the differential responses between the expressed receptor (Sf9 cells) and the endogenous receptor (pheromone glands) emphasize the importance of assaying lead compounds using several alternative bioassays (at the cellular, tissue and organism levels). The surprising discovery of the presence of the gene encoding the PBAN-R in the male homologous tissue, but its absence at the protein level, launches opportunities for studying molecular regulation pathways and the evolution of these GPCRs. Overall this research will advance research towards the goal of finding antagonists for this important class of receptors that might encompass a variety of essential insect functions.
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