Dissertations / Theses on the topic 'Diabetes – Etiology'
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Topp, Brian G. "The etiology and natural history of type 2 diabetes /." Burnaby B.C. : Simon Fraser University, 2005. http://ir.lib.sfu.ca/handle/1892/2423.
Full textFerreira, Filipa Cristina Costa. "A Diabetes - Principais Parâmetros para o Controlo da Diabetes." Master's thesis, Faculdade de Ciências Médicas. UNL, 2013. http://hdl.handle.net/10362/10001.
Full textOrr, Neil John. "Patterns of care for diabetes: risk factors for vision-threatening retinopathy." Thesis, The University of Sydney, 2005. http://hdl.handle.net/2123/1421.
Full textOrr, Neil John. "Patterns of care for diabetes: risk factors for vision-threatening retinopathy." University of Sydney, 2005. http://hdl.handle.net/2123/1421.
Full textOBJECTIVES: In Australia, diabetes causes significant morbidity and mortality. Whilst the need to prevent diabetes and its complications has been widely recognised, the capacity of health care systems - which organise diabetes care - to facilitate prevention has not been fully established. METHODS: A series of seven population-based case-control studies were used to examine the effectiveness of the Australian health care system and its capacity to manage diabetes. Six of the studies compared the patterns of care of patients who had developed advanced diabetes complications in 2000 (cases), to similar patients who remained free of the condition (controls) across Australia and for various risk groups. A secondary study investigated the role of treating GPs in the development of the outcome. RESULTS: A strong relationship between the patterns of care and the development of advanced diabetes complications was found and is described in Chapter 4. In Chapter 5, this same relationship was investigated for each Australian state and territory, and similar findings were made. The study in Chapter 6 investigated whether late diagnosis or the patterns of care was the stronger risk factor for advanced diabetes complications, finding that the greatest risk was associated with the latter. In Chapter 7 the influence of medical care during the pre-diagnosis period was explored, and a strong relationship between care obtained in this period and the development of advanced complications was found. In Chapter 8, which investigated the role of socio-economic status in the development of advanced complications, found that the risk of advanced diabetes complications was higher in low socio-economic groups. Chapter 9 investigated geographic isolation and the development of advanced diabetes complications and found that the risk of advanced complications was higher in geographically isolated populations. Finally, Chapter 10, which utilised a provider database, found that some GP characteristics were associated with the development of advanced diabetes complications in patients. CONCLUSION: A number of major risk factors for the development of advanced complications in Australia was found. These related to poorer diabetes management, later diagnosis, low socioeconomic status and geographic isolation. Strategies must be devised to promote effective diabetes management and the early diagnosis of diabetes across the Australian population.
Marshall, Julie Ann. "The role of dietary fiber in the etiology of noninsulin-dependent diabetes mellitus /." Thesis, Connect to this title online; UW restricted, 1987. http://hdl.handle.net/1773/10952.
Full textTuratti, Luiz Alberto Andreotti. "Papel dos componentes do sistema GH-IGF-IGFBP nos mecanismos envolvidos na resposta imunológica do Diabetes Melito tipo 1." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-10102014-114845/.
Full textAiming to verify if GH-IGF-IGFBP proteins system and insulin-like growth factor type I receptor (IGF-IR) are implicated on pathofisiology of type 1 Diabetes Mellitus (DM1), we studied 23 prepubertal patients with DM1 on different stages of diagnosis (Group A: time of diagnosis <= 6 months; Group B: time of diagnosis > 6 months) and 10 prepubertal healthy subjects as control group (Group C). The RT-PCR molecular assay for IGF-IR mRNA on peripheral T and B lymphocytes didn\'t show statistical differences between the groups when T cells were analyzed. We found an increase of IGF-IR mRNA expression on B cells from diabetic patients when compared to healthy subjects (p< 0,05). There were no differences in the GH-IGF-IGFBP proteins system levels between the groups. Our study suggest that IGF-IR in association with diabetes-related autoantibodies (ICA, anti-GAD and anti-IA2) presence could activate B cells involved on pathofisiology of DM1
Agardh, Emilie. "The influence of psychosocial stress, socioeconomic differences and coffee consumption in the etiology of type 2 diabetes /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-180-6/.
Full textLewandowski, Paul, and mikewood@deakin edu au. "Liver fat metabolism, obesity and diabetes in Psammomys Obesus." Deakin University. School of Health Sciences, 1999. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20050825.111432.
Full textRodrigues, Brian Baltzar. "Hypertension and diabetic cardiomyopathy." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/24906.
Full textPharmaceutical Sciences, Faculty of
Graduate
Stevens, Joseph. "Coxsackievirus Infection of B Cells: Towards a Better Understanding of the Etiology of Type 1 Diabetes." University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1522418340151761.
Full textKäräjämäki, A. (Aki). "Non-alcoholic fatty liver disease (NAFLD):perspectives to etiology, complications and lipid metabolism." Doctoral thesis, Oulun yliopisto, 2017. http://urn.fi/urn:isbn:9789526217376.
Full textTiivistelmä Maailmanlaajuisesti noin 25% täysi-ikäisistä henkilöistä sairastaa alkoholinkäyttöön liittymätöntä rasvamaksaa. Sen tiedetään altistavan sydän- ja verisuonisairauksille, aineenvaihduntahäiriöille, maksakirroosille ja jopa maksasyövälle, mutta elämäntapahoitoa lukuun ottamatta hoitomahdollisuudet ovat toistaiseksi vähäisiä. Tässä väitöskirjassa osoitetaan ensimmäistä kertaa alkoholinkäyttöön liittymättömän rasvamaksan ennustavan itsenäisesti eteisvärinän ilmaantuvuutta noin 16 vuoden seurannan aikana 958 tavallisen keski-ikäisen ihmisen aineistossa osana OPERA-tutkimusta. Lisäksi väitöskirjassa osoitetaan maksan sidekudosmuodostuksen ja eteisvärinän välillä olevan yhteys poikkileikkausasetelmassa 76 iäkkään ihmisen muodostamassa aineistossa. Väitöstutkimuksessa havaittiin myös, että metabolista oireyhtymää sairastavilla henkilöillä on suurentunut tyypin 2 diabeteksen, sydän- ja verisuonisairauksien sekä vasemman kammion koon suurentumisen riski noin 20 vuoden seurannan aikana 958 tutkittavan henkilön aineistossa riippumatta siitä, onko heillä alkoholinkäyttöön liittymätön rasvamaksa. Toisin sanoen alkoholin käyttöön liittymätön rasvamaksa ilman metabolista oireyhtymää ei lisää edellä mainittujen kolmen sairauden riskiä. Väitöstutkimuksessa esitetään lisäksi, että rifampisiinilla aikaansaatu maksan pregnane X -reseptorin aktivaatio johtaa seerumin fosfolipidien, tiettyjen rasvahappojen sekä usean eri kolesterolityypin lisääntymiseen 34 terveen nuoren henkilön aineistossa. Kirjallisuudessa näiden seerumin rasva-aineiden on esitetty aiheuttavan alkoholin käyttöön liittymätöntä maksatulehdusta ja jopa rasvamaksan vakavimpia muotoja. Toisaalta rifampisiini ei lisännyt seerumin triglyseridipitoisuutta eikä aiheuttanut magneettitutkimuksella mitattuna triglyseridien kertymistä maksaan 15 terveen nuoren henkilön aineistossa. Tämä väitöstutkimus antaa lisätietoa rasvamaksan kehittymisestä, rasva-aineenvaihdunnasta ja komplikaatioista sekä korostaa rasvamaksan monimuotoista luonnetta. Nämä löydökset saattavat parantaa rasvamaksaa sairastavien henkilöiden hoitoa ja seurantaa
Cardinal, John William. "The basis for strain variation to the diabetogenic effects of streptozotocin in mice." Thesis, Queensland University of Technology, 1997.
Find full textTimóteo, Ana Teresa de Matos. "Caracterização da aterosclerose sub-clínica e doença coronária em doentes com síndrome metabólica e diabetes mellitus." Doctoral thesis, Faculdade de Ciências Médicas. UNL, 2013. http://hdl.handle.net/10362/10304.
Full textElmansy, Dalia F. "Computational Methods to Characterize the Etiology of Complex Diseases at Multiple Levels." Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1583416431321447.
Full textSellers, Elizabeth A. C. "The emerging epidemic of type 2 diabetes mellitus in First Nation children and youth, issues related to diagnosis, etiology, complications and treatment." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ56146.pdf.
Full textParween, Saba. "Diabetes in 3D : β-cell mass assessments in disease models & evaluation of SPECT based imaging." Doctoral thesis, Umeå universitet, Umeå centrum för molekylär medicin (UCMM), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-127550.
Full textHammarbäck, Madelene. "Development of a dynamic ex vivo culture system for human islets of langerhans." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-353398.
Full textMa, Zhi. "Islet amyloid polypeptide (IAPP) : mechanisms of amyloidogenesis in the pancreatic islets and potential roles in diabetes mellitus /." Linköping : Univ, 2001. http://www.bibl.liu.se/liupubl/disp/disp2001/med655s.pdf.
Full textMalek-Ahmadi, Michael. "Cardiovascular risk factors for mild cognitive impairment." [Tampa, Fla] : University of South Florida, 2009. http://purl.fcla.edu/usf/dc/et/SFE0002872.
Full textNordfeldt, Sam. "On Severe Hypoglycaemia in Children and Adolescents with Type 1 Diabetes." Doctoral thesis, Linköping : Univ, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-5018.
Full textRobinson, Jacquelyn Patricia Price. "Sociocultural Risk Factors of Non-Insulin Diabetes Mellitus Among Middle Class African Americans in Central Ohio." Columbus, OH : Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1047487253.
Full textTitle from first page of PDF file. Document formatted into pages; contains xviii, 233 p.: ill. Includes abstract and vita. Advisor: Douglas E. Crews, Dept. of Anthropology. Includes bibliographical references (p. 209-233).
Sepa, Anneli. "The Stress Hypothesis : Implications for the induction of diabetes-related autoimmunity in children?" Doctoral thesis, Linköping : Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-5177.
Full textChaves, Áurea Jacob. ""Análise volumétrica da hiperplasia intimal intra-stent em pacientes diabéticos tratados com e sem abciximab"." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/5/5131/tde-09082005-113017/.
Full textNinety-six type 2 diabetics were randomly assigned to receive abciximab or no abciximab at the time of elective stent implantation to determine whether this IIb/IIIa glycoprotein inhibitor would reduce in-stent intimal hyperplasia, measured by intravascular ultrasound, at 6-month follow-up. Volumetric analysis showed that abciximab was not associated with a reduction of in-stent volume obstruction in diabetic patients [41.3% (DP21.0%) versus 40.5% (DP18.3%), p=0.853).
Körner, Anna. "Pathomechanism of diabetic nephropathy /." Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3385-5/.
Full text傅子穎 and Tsi-wing Fu. "The expression and regulation of genes that may contribute to the etiology of diabetic neuropathy in mouse." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215245.
Full textFu, Tsi-wing. "The expression and regulation of genes that may contribute to the etiology of diabetic neuropathy in mouse /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19872057.
Full textLem, Kristina Yvonne. "Evaluation of dietary factors associated with spontaneous pancreatitis in dogs." [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-1504.
Full textBravo-Egaña, Valia. "Perfil de expressão de miRNAs endócrinos em ilhotas pancreáticas : modulação da sua expressão por citocinas proinflamatórias associadas com a etiologia da Diabetes tipo 1." reponame:Repositório Institucional da UnB, 2008. http://repositorio.unb.br/handle/10482/2685.
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MicroRNAs (miRNAs) são os produtos gênicos não-codantes (~19-22 nucleotídeos) com um papel chave no regulamento pós-transcricional inibindo a expressão gênica através da união seletiva a seqüências complementares de RNA mensageiro. Nossos resultados indicam que os miRNAs isolados com métodos atualmente usados para preparação de RNA são instáveis assim como também seus cDNAs correspondentes. Estas observações têm grande importância em estudos de análise de expressão de miRNAs. Além, nosso laboratório é pioneiro em determinar perfis específicos de miRNA em ilhotas pancreáticas humanas e de roedores normais assim como em ilhotas expostas à ação de citocinas diabetogênicas pró-inflamatórias. O miR-7 resultou ser o miRNA mais abundante da porção endócrina das ilhota e o miR-375 o mais abundante no pâncreas. Ambos os miRNAs aumentaram sua expressão durante o desenvolvimento pancreático, assim como também, em ilhotas tratadas com citocinas pró-inflamatórias por 6 horas. Estas observações sugerem que o miR-7 e o miR-375 desempenham um papel importante não apenas durante o processo de desenvolvimento e de manutenção do fenótipo das ilhotas mas também nos sinais de sobrevivência nos primeiros estágios da resposta inflamatória. A pesquisa dos miRNAs e de seus mRNAs alvos pode tornar-se uma ferramenta valiosa para o diagnóstico e tratamento de doenças tais como o diabetes. ___________________________________________________________________________________ ABSTRACT
MicroRNAs (miRNAs) are non-coding gene products (~19–22 nucleotides) that play a key role in post-transcriptional regulation by inhibiting gene expression through selective binding to complementary messenger RNA sequences. Our early results indicated that miRNAs isolated with currently used methods for RNA preparation are unstable and so are their corresponding cDNAs. These observations should be of great interest to all researches that outsource RNA samples for miRNA gene array analysis. We are first to determine specific miRNA signatures in normal pancreatic islets as well as in islets exposed to pro-inflammatory (diabetogenic) cytokines. MiR-7 emerged as the most abundant endocrine islet miRNA and miR-375 as the most abundant intra-islet miRNA. Both miRNAs increased their expression during pancreatic development as well as in islets treated with pro-inflammatory cytokines for 6 hours. This suggests that miR-7 and miR-375 play an important role not only in development and islet phenotype maintenance but also in the survival signaling at the early inflammatory response. The research of miRNAs and their target mRNAs could be a valuable tool in diagnostics by indicating the early onset of diseases such as diabetes and by finding new therapeutic targets to treat them.
Zaslavsky, Lerida Maria Araujo. "Disfunção cognitiva em pacientes diabéticos não insulino-dependentes com neuropatia autonômica." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 1994. http://hdl.handle.net/10183/171790.
Full textAbnormal cognitive function in Type 2 diabetic patients has been associated with peripheral neuropathy, poor metabolic contrai and a non-specific effect of chronic disease. A cross-sectional controlled study was conducted to determine whether autonomic neuropathy (AN), defined according to cardiovascular tests as proposed by Ewing, is independently associated with cognitive dysfunction in Type 2 diabetic patients. Study participants were 20 Type 2 diabetic patieuts witb AN (14 males and 6 females; age = 60±1 years); 29 Type 2 diabetic patients without AN (14 males and 15 females; age = 59±1 years) aud 34 non diabetic patients with non-inflammatory articular disease (10 males and 24 females; age = 58± 1 years), matched by age, educational level and duration of disease. Cognitive functiou was evaluated by tests of verbal - immediate and receut (digit span; word span), visual - immediate and recent (recognition of silhouettes of towers and famous faces) and remate memory (dates of important events). Diabetic patients with AN obtained significantly lower scores in visual memory tests than diabetic patients without AN and controls (towers immediate = 7 versus 6 versus 6; towers recent = 4 versus 6 versus 6; faces = 16 versus 18 versus 18, respectively; Kruskal-Wallis; p < 0.05). Talking into account other factors (age, educational level, duration of disease and fasting plasma glucose) that could interfere with performance in cognitive tests, stepwise multiple regression analysis disclosed that performance in visual tests remained significantly associated to AN (p = 0,0054; partial r2 = 0,166 aud p = 0,0076; parcial r2 = 0,163 for TOWER 1 and TOWER F, respectively). Negative correlations (Spearman; r = -0,25 and r= -0, 24) were observed between the scores in visual memory tests (FACES and TOWER F, respectively) and the number of abnormal cardiovascular tests. In conclusion, decreased visual cognitive function in Type 2 diabetic patients is associated to the presence and degree of AN.
Caramori, Maria Luiza Avancini. "Valor da excreção urinária de allbumina como marcador de risco para o desenvolvimento de Nefropatia Diabética e relação entre a estrutura glomerular e a função renal em pacientes com Diabete Melito." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2001. http://hdl.handle.net/10183/1740.
Full textDalmaz, Caroline Abrão. "Polimorfismos de inserção/deleção do gene da ECA e K121Q do gene PC-1 em pacientes com Diabete Mellito tipo 1 normoalbuminúricos : estudo com 10 anos de acompanhamento." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2001. http://hdl.handle.net/10183/2697.
Full textThe aim of this study was to analyze the role of the ACE gene insertion/deletion (I/D) polymorphisms and of the PC-1 gene K121Q polymorphism in the changes of glomerular filtration rate (GFR), urinary albumin excretion rate (UAER), and blood pressure levels in a cohort of normoalbuminuric type 1 diabetic patients. This was a 10.2 ± 2.0 year prospective study of 30 normotensive normoalbuminuric type 1 diabetic patients. UAER (immunoturbidimetry), GFR (51Cr-EDTA single injection technique), GHb (ion-exchange chromatography) and blood pressure levels were measured at baseline and at 1.7 ± 0.6 year intervals. The presence of ACE gene I/D and PC-1 gene K121Q polymorphisms was determined by polymerase chain reaction and restriction enzyme techniques. Eleven patients was a II genotype, 13 the ID and 6 was the DD genotype. Three patients developed diabetic nephropathy; all were carriers of allele D of the ACE gene. The presence of allele D was the only predictor (R2=0.15; F=4.92; P=0.035) of the observed GFR decline (-0.29 ± 0.34 ml/min/month; P<0.05). UAER increased during the study (log UAER change = 0.0275 ± 0.042 μg/min/month; P=0.002) and was associated with baseline UAER levels only (R2=0.17; F=5.72; P=0.024). A significant increase (P<0.05) in cases of hypertension and new cases of retinopathy were observed only ID/DD and not in II patients. Twenty-two patients was KK genotype, 7 the KQ and 1 was the QQ genotype. Patients with the KQ/QQ (n=8) presented a significant increase (P=0.045) in new cases of retinopathy. In conclusion the presence of D allele of ACE gene in this sample of normoalbuminuric normotensive type 1 diabetes patients was associated with a higher proportion of microvascular complications and hypertension.
Caramori, Maria Luiza Avancini. "Fatores de risco para o desenvolvimento de nefropatia diabética e alterações presssoricas em pacientes com diabete melito tipo 1, normoalbuminicos e normotensos : estudo com 8 anos de acompanhamento." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 1997. http://hdl.handle.net/10183/171776.
Full textFriedman, Rogério. "Contribuições ao estudo da nefropatia diabética : estudo evolutivo da função renal em pacientes proteinuricos; efeito da qualidade de proteina na função renal de pacientes microalbuminuricos; atividade do sistema de contratransporte de sodio-litio." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 1995. http://hdl.handle.net/10183/164518.
Full textProteinuria (micro- and macroalbuminuria) identifies diabetic patients with nephropathy and/or those at risk of cardiovascular disease and death. The three studies that are part of this work explore aspects o f the evolution of kidney function, possibilities of early intervention, and markers of predisposition for these complications.
Mendonça, Juliana Maria Dantas. "Avaliação do efeito da intervenção farmacêutica no controle da glicemia de pacientes ambulatoriais portadores do diabetes mellitus tipo 2." Universidade Federal de Sergipe, 2013. https://ri.ufs.br/handle/riufs/3801.
Full textO diabetes mellitus (DM) é uma síndrome de etiologia múltipla decorrente da falta de insulina e/ou da incapacidade da insulina exercer adequadamente seus efeitos. É considerado, mundialmente, um problema de saúde pública pela posição epidemiológica que ocupa com altas taxas de incidência e prevalência, além de acarretar complicações macrovasculares e microvasculares. O DM apresenta duas formas principais, o tipo 1 (DM1), que aparece principalmente na infância ou na adolescência e o tipo 2 (DM2), a mais freqüente, responsável por 85% a 90% dos casos, geralmente de instalação insidiosa, principalmente após os 40 anos de idade, acometendo indivíduos obesos em 90% das vezes. As doenças cardiovasculares (DCV) são responsáveis por aproximadamente 52% das mortes dos portadores de DM. A estratégia de prevenção destas complicações crônicas dependem, fundamentalmente, do adequado controle da glicemia e de outras comorbidades, entre elas a dislipidemia e a hipertensão arterial sistêmica (HAS). A presente ivestigação foi conduzida visando avaliar o efeito da intervenção farmacêutica no controle da glicemia de pacientes ambulatoriais portadores de DM2.Trata-se de um estudo longitudinal com intervenção, utilizando-se 100 sujeitos durante consulta, consecutivamente, com diagnóstico de DM2 em ambulatório privado de endocrinologia no período de maio de 2011 a fevereiro de 2012. Todos os voluntários responderam a um questionário e sofreram intervenção farmacêutica, realizada pelo pesquisador. Após esta intervenção, ocorreu uma redução significativa de 45% IC 95% nos níveis de A1C. Diante disso, observou-se ainda melhora nos resultados entre as mulheres (69%) (p=0,01) e nos seguintes parâmetros: glicemia de jejum (p=0,000), frequência de exercícios físicos (p=0,0001), adoção da dieta hipocalórica (p= 0,0001), adesão à terapia medicamentosa (p= 0,024) e IMC (p= 0,012).
Chen, Carla Chia-Ming. "Bayesian methodology for genetics of complex diseases." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/43357/1/Carla_Chen_Thesis.pdf.
Full textManna, Thais Della. "Avaliação do comprometimento endócrino do pâncreas em crianças e adolescentes portadores de fibrose cística." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-11042007-112815/.
Full textINTRODUCTION: Cystic fibrosis-related diabetes is a common complication leading to clinical deterioration of these patients. Aiming at an earlier diagnosis, we investigated the kinetics of the glucose-metabolism abnormalities by evaluating glucose, insulin, pro-insulin, C-peptide and glucagon responses after oral glucose (OGTT) and a mixed meal tolerance (MMTT) tests. METHODS: In a cross-sectional and controlled study, conducted from july/2004 till august/2005, 52 children and adolescents with cystic fibrosis, from 5 to 19 years old, underwent both tests in an interval of less than 10 weeks. RESULTS: Plasma glucose values were significantly lower during MMTT after 60 minutes and insulin, C-peptide and glucagon were secreted earlier, being significantly higher at 30 minutes. During OGTT, patients showed a delayed but higher peak insulin, C-peptide and pro-insulin secretion at time 120 minutes. Patients with Cystic Fibrosis-Related Diabetes (CFRD) without fasting hyperglycemia presented insulin, C-peptide and proinsulin values significantly higher than those patients with normal glucose tolerance or pre-diabetes, at time 120 minutes, indicating increased peripheral insulin resistance. An overlap of insulin, C-peptide and pro-insulin levels was observed in normal and pre-diabetic patients as well as in people with CFRD with fasting hyperglycemia, at 120 minutes, suggesting beta cell dysfunction in the latter groups. Conclusion: Mixed meal ingestion caused an earlier hormone secretion stimulated probably by nutrients different from glucose. Insulin resistance besides beta cell dysfunction are involved in the pathogenesis of cystic fibrosis related glucose disturbances.
SILVA, Marília Santana da. "Trajetória Assistencial de um Evento Sentinela: Avaliação da atenção integral ao usuário diabético no SUS a partir da Retinopatia Diabética Grave." reponame:Repositório Institucional da FIOCRUZ, 2015. http://www.arca.fiocruz.br/handle/icict/15820.
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Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
A prevalência das condições crônicas cresce vertiginosamente na população. Dentre elas, destaca-se o diabetes, sendo o agravo responsável por incapacidades e mortes, com elevados custos financeiros e sociais. Os muitos casos de complicações do diabetes evidencia a existência de falhas na atenção à saúde dos usuários. Diante desse contexto, se faz necessário a mudança na organização da atenção à saúde, com vistas à atenção integral, intencionando transcender a racionalidade estritamente biomédica e oferecer respostas efetivas às suas necessidades de saúde. Esse estudo se propôs a avaliar a atenção integral ao usuário diabético no município de Recife, a partir das trajetórias assistenciais dos diabéticos complicados com retinopatia diabética grave, considerada como uma complicação evitável do diabetes e, portanto, um indicador de monitoramento de emergência ou evento sentinela. Foram identificados 4 usuários com retinopatia diabética no Centro Médico, serviço de referência para o portador de diabetes, que foram entrevistados através da técnica em profundidade. As trajetórias dos usuários foram reconstruídas, sendo que os mesmos foram renomeados com termos que melhor expressam o sentimento mais explícito em cada um dos caminhos. As entrevistas foram analisadas a partir da Técnica da História de Vida, com foco nas categorias pré-estabelecidas: acesso e utilização de serviços; atendimento humanizado: vínculo, responsabilização e acolhimento; e coordenação e ordenação do cuidado. Identificaram-se fragilidades na atenção dos usuários, destacando-se ainda a quase inexistente relação entre os entrevistados e a atenção básica, o que prejudica ainda mais a garantia da assistência integral. As evidências encontradas embasam a afirmativa de que os usuários diabéticos não são assistidos de forma integral, mantendo-se a assistência fragmentada e focada nas “agudizações” da doença. Uma atenção à saúde não adequada possibilita o surgimento de complicações evitáveis, como a retinopatia diabética e outras. Faz-se necessário uma mudança na organização da atenção à saúde do diabético, a fim de alcançar uma assistência integral e resolutiva para o mesmo, que se traduza em redução da prevalência das complicações do agravo e de seu impacto social (AU)
Rautio, Aslak. "Diabetes Mellitus and Cardiovascular Risk : epidemiology, etiology and intervention." Doctoral thesis, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-117028.
Full textDiabetes bakgrund: Diabetes Mellitus är en av de vanligaste kroniska sjukdomarna i världen. Diabetesförekomsten har ökat påtagligt de senaste årtiondena. Ökningen beror på ökat insjuknande på grund av livsstilsförändringar med minskat fysisk aktivitet och ökad övervikt och även på grund av förbättrad diagnostik. Även förbättrad sjukvård med ökad överlevnad hos patienter med diabetes samt fler andel äldre ökar andelen diabetes patienter i befolkningen. Enligt WHO har antalet patienter med diabetes stigit från 30 miljoner år 1985 till 171 miljoner år 2000 då uppskattningsvis 4,6 % av vuxenbefolkning har diabetessjukdomen. Majoriteten, ca 80 % har diabetes typ 2. WHO:s prognos visar fortsatt kraftig ökning av diabetes med ca 550 miljoner drabbade år 2030. Majoriteten av denna förändring förklaras av diabetes typ 2 som i framtiden beräknas utgöra 90-95% av all diabetes. Diabetes är fortsatt den viktigaste bakomliggande orsaken till terminal njursvikt i industriländer och en av de vanligaste orsakerna till blindhet i världen. Diabetes innebär en kraftigt ökad risk för hjärt- och kärlsjukdomar. Patienter med diabetes som drabbats av hjärt- och kärlsjukdom såsom hjärtinfarkt eller stroke har ofta sämre prognos, både akut och på långsiktigt, jämfört med patienter utan diabetes. På grund av att diabetes är en kronisk sjukdom med många, ofta svåra och kostsamma, komplikationer står diabetesrelaterade sjukvårdskostnader för en betydande del av totala de sjukvårdskostnaderna i världen varierande mellan 2,5 – 25 % av olika länders sjukvårdsbudget. Diabetes och risk för hjärt- och kärlsjukdomar: De klassiska risk-faktorerna för hjärt- och kärlsjukdomar är ålder, rökning, högt blodtryck, högt kolesterol, låg fysisk aktivitet/övervikt och ärftlighet. Lägger man till diabetes till dessa riskfaktorer får man påtagligt ökad risk för kärlsjukdomar som hjärtinfarkt, stroke och perifer kärlsjukdom. Mekanismerna bakom denna överrisk av diabetes är fortfarande åtminstone till viss del oklara och aktuella för intensiv forskning och debatt. Sannolikt har tiden före diabetesdiagnosen, prediabetes, betydelse då vi vet att vid tidpunkt för diagnos har patienterna med typ 2 diabetes haft störd sockeromsättning varierande länge, oftast sannolikt flera år. Andra tänkbara diabetesrelaterade orsaker för ökad hjärt- och kärlsjuklighet kan vara kronisk inflammation, blodets störda levringsfunktion (hypofibrinolys), nedsatt funktion i blodkärlen (endoteldysfunktion), ogynnsam blodfetts-profil, kroniskt höga insulinnivåer och även sannolikt direkt skadlig effekt av det höga sockret på flertal celler och organ i kroppen. Framtidens behandlingsmöjligheter: Aktuella behandlingsriktlinjer betonar vikten av att betrakta individer med diabetes som högriskpersoner avseende hjärt- och kärlsjukdomar oavsett förekomst av känd kärlsjukdom. Sjukvården ska primärpreventivt intensivbehandla de traditionella riskfaktorerna hos individer med diabetes. Det är dock uppenbart att personer även med helt optimalt behandlade riskfaktorer som blodtryck och kolesterol och även optimal sockerkontroll har fortsatt högre risk för hjärt- och kärlsjukdomar, så kallad residual risk, jämfört med personer utan diabetes. Det är viktigt att i framtiden försöka klargöra vilka faktorer som utgör denna residual risk och utveckla behandlingsmöjligheter mot dessa faktorer. Det är sannolikt av största vikt att tidigt identifiera individer med störd sockeromsättning och risk för utveckling av diabetes. Sjukvården bör sträva efter att minimera de negativa effekterna av prediabetes och förebygga progress till manifest diabetes. Avhandlingens syfte: Att kartlägga hur effektiv den traditionella riskfaktorbehandlingen har varit i att förebygga hjärt- och kärlsjukdomar, särskilt hjärtinfarkt och stroke, hos patienter med diabetes. I avhandlingen studeras närmare en av de icke traditionella riskfaktorerna nämligen i det blodproppsupplösande systemet, fibrinolysen. Det är numera välkänt att diabetes i sig påverkar fibrinolyssystemet ogynnsamt och att vissa diabetesläkemedel kan påverka fibrinolysen gynnsamt. Vi har studerat om förändringar i fibrinolysen kan prediktera perifer kärlsjukdom hos patienter med diabetes och om intensiv insulinbehandling kan påverka detta fibrinolyssystem. Resultat: Avhandlingen visar att insjuknandet och dödlighet i hjärtinfarkt och stroke har minskat i Västerbotten och i Norrbotten. För stroke var det första gången ett minskat insjuknande kunde rapporteras för denna population. Tyvärr har inte patienter med diabetes och hjärtinfarkt fått ta del av dessa gynnsamma trender under studerad tid. För stroke har kvinnor med diabetes fått minskat insjuknande men inte män med diabetes. Strokestudien kunde också redovisa minskat dödlighet i stroke för alla patienter utom kvinnor med diabetes med en förstagångs insjuknande i stroke. Patienter med diabetes har påverkad fibrinolys jämfört med personer med normal glukosmetabolism. Även patienter med tidigare hjärtinfarkt uppvisar ett hypofibrinolytisk tillstånd med låg tPA-aktivitet och höga nivåer av PAI-1 och fibrinogen. Arbete III visade en association mellan låg tPA-aktivitet och höga nivåer av fibrinogen hos patienter med förstagångs hjärtinfarkt. Detta samband kvarstod även efter justering med traditionella riskfaktorer. Arbete IV, en 10 års uppföljning visade hos patienter med diabetes att tPA-aktivitet kan prediktera förekomsten perifer kärlsjukdom vid baslinjen och även vid 10-års uppföljning. Dessutom var tPA-mass vid 10-års uppföljning associerad med perifer kärlsjukdom. Av de traditionella riskfaktorerna var ålder, hypertension och HbA1c vid studiestart oberoende associerade med förekomst av perifer kärlsjukdom vid 10 års uppföljning. Denna långtidsstudie stöder tidigare fynd av ett signifikant samband mellan asymtomatisk perifer kärlsjukdom och tPA-aktivitet. Sålunda kan tPA-aktivitet vara en tidig markör av perifer kärlsjukdom, även om, den bakom liggande mekanismen för detta är fortfarande oklart. Flera större studier rapporterar motstridiga resultat beträffande för- och nackdelarna med intensiv insulinbehandling. ORIGIN studien var en internationell multicenterstudie som undersökte effekterna av intensiv insulinbehandling på hjärt- och kärlsjukdomar. I arbete V med ORIGIN-studiens svenska kohort undersöktes effekterna av insulinbehandling på fibrinolys. Randomisering till insulinbehandling hade ingen signifikant effekt på de studerade fibrinolytiska markörerna eller von Willebrand faktorn VWF jämfört med standardbehandling. Logaritmerade medeldeltavärden mellan baslinjen och studie slutet ökade signifikant för tPA-aktivitet och tPA/PAI-1-komplexet. För PAI-1, tPA-antigen och för VWF kunde inga signifikanta skillnader visas. Inom-gruppsanalys över hela studieperioden visade signifikanta förändringar för tPA/PAI-1-komplexet, tPA-antigen och VWF i insulingrupp men inga signifikanta förändringar för patienter med standardbehandling. Hypotesen att randomisering till insulinbehandling skulle förbättra nivåerna av fibrinolysfaktorer eller VWF jämfört med standardbehandling kunde inte verifieras Konklusion: Denna avhandling visar att patienter med diabetes har fortsatt ökad risk för hjärtinfarkt och stroke jämfört med individer med normal sockeromsättning. Denna överrisk betyder för patienter med diabetes en ökad risk för insjuknande, svårare sjukdomstillstånd och sämre prognos med ökad risk för återinsjuknande samt även högre dödlighet både på kort och lång sikt. Även andra aktuella studier rapporterar en fortsatt hjärt- och kärlrelaterad överrisk hos patienter med diabetes. Fibrinolyssystemet har betydelse för hjärt- och kärlsjukdomar och denna avhandling visar att patienter med diabetes har ogynnsamma förändringar i sin fibrinolys, så kallad hypofibrinolys. Intensiv insulinbehandling förefaller inte påverka fibrinolysen till den grad att minskad hjärt- och kärlsjuklighet kan ses.
"Phenotypic and genotypic characterization of nephropathy in Chinese patients with type 2 diabetes." 2003. http://library.cuhk.edu.hk/record=b6073540.
Full text"July 2003."
Thesis (Ph.D.)--Chinese University of Hong Kong, 2003.
Includes bibliographical references (p. 250-297).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Mode of access: World Wide Web.
Abstracts in English and Chinese.
Gazda, Lawrence S. "Studies in autoimmune diabetes." Phd thesis, 1997. http://hdl.handle.net/1885/145302.
Full text"Global human transcriptomic variation." 2012. http://library.cuhk.edu.hk/record=b5549499.
Full textExtensive intra- and inter- ethnic transcriptome variation reflects human adaptation and natural selection. Gene expression is the primary mechanism that translates genome information into functional gene product that lead to physiological phenotypes. Aberrant gene expression has been associated to the pathogenesis of diseases. The genome revolution has offered unique opportunity for a comprehensive interrogation of the complexity of human transcriptome. Analysis of transcriptome using RNA-Seq requires sophisticated bioinformatics approach. In a technical perspective, an empirical model based on the geometric-tail distribution of intron lengths in mammalian genome was developed to accurately determine splice junctions from junction reads and locations of non-uniquely mapped reads. Such method handles non-uniquely mapped reads better than BWA. The method can also detect more experimentally confirmed splice junction than other tools. Expressional phenotyping was employed to explore global transcriptomic variation between Beijing Han Chinese and Western European. In addition to inter-ethnic variations in gene expression, ethnic specific splice juctions were found. Further, ethnic specific trait manifests in differential relative isoform abundance. Lastly, such spectrum of variations was different between different ethnic groups, suggesting alternative splicing as another molecular phenotype that delineates ethnic diversity. Expressional phenotyping was then used in a case-control study to explore the molecular abnormalities of a complex disease: Type 2 Diabetes (T2DM). T2DM manifested in wide-spread repression of gene expression. The study (1) confirmed previously reported Genome-wide Association Study (GWAS) loci; (2) curated poorly characteriezed GWAS loci and (3) discovered novel T2DM associated genes. By integrating various alteration signals and interpretation performed in a highly confident gene-gene interaction network, this study augmented the understanding of expressed abnormalities in T2DM. The results were validated in a broader 69 x 79 case-control group.
Detailed summary in vernacular field only.
Li, Jing Woei.
Thesis (Ph.D.)--Chinese University of Hong Kong, 2012.
Includes bibliographical references (leaves 118-130).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstract also in Chinese.
Abstract --- p.v
中文擇要 --- p.vi
Thesis/Assessment Committee --- p.ix
Acknowledgement --- p.ix
List of figures --- p.x
List of tables --- p.xii
List of Abbreviations --- p.xiii
Scientific contributions --- p.xv
List of Publication(s) related to this thesis --- p.xvi
Conference presentations --- p.xvii
Chapter Chapter 1: --- Introduction and Literature Reviews --- p.1
Chapter 1.1 --- The variable human transcriptome --- p.1
Chapter 1.2 --- Significance of variation in gene expression and transcript variants --- p.2
Chapter 1.3 --- Transcriptomic study in a technological perspective --- p.8
Chapter 1.3.1 --- Microarray: Probing what was designed to be probed --- p.8
Chapter 1.3.2 --- RNA-Seq: the ab initio decoder of biological sequences --- p.9
Chapter 1.4 --- Analysis of RNA-Seq data --- p.10
Chapter 1.4.1 --- The bioinformatics challenges prevail --- p.10
Chapter 1.4.2 --- Identifying changes in gene expression --- p.16
Chapter 1.4.3 --- Identifying splice site, quantification of isoform level expression --- p.17
Chapter 1.5 --- Conclusion --- p.19
Chapter 1.6 --- Aims of this study --- p.20
Chapter 1.6.1 --- Splice junction determination --- p.20
Chapter 1.6.2 --- Expressional phenotyping in ethnical context --- p.20
Chapter 1.6.3 --- Expressional phenotyping in a disease context --- p.20
Chapter Chapter 2: --- Detection of splicing events --- p.21
Chapter 2.1 --- Abstract --- p.21
Chapter 2.2 --- Introduction --- p.22
Chapter 2.3 --- Methods and workflow --- p.25
Chapter 2.4 --- Algorithm --- p.29
Chapter 2.5 --- Geometric-tail distribution --- p.32
Chapter 2.6 --- Insert-size distribution --- p.33
Chapter 2.7 --- Multiread analysis --- p.34
Chapter 2.7.1 --- GT model probably places multiread more accurately than BWA --- p.35
Chapter 2.8 --- Splice-site comparison --- p.37
Chapter 2.8.1 --- GT model discovers more experimentally confirmed splice junction --- p.37
Chapter 2.8.2 --- GT model is highly accurate --- p.39
Chapter 2.9 --- Discussion --- p.40
Chapter 2.10 --- Limitation --- p.40
Chapter Chapter 3: --- Transcriptomic variation in a ethnicity context --- p.41
Chapter 3.1 --- Abstract --- p.41
Chapter 3.2 --- Introduction --- p.42
Chapter 3.3 --- Materials and Methods --- p.46
Chapter 3.3.1 --- HapMap lymphoblastoid cell-lines --- p.46
Chapter 3.3.2 --- Sequenced samples --- p.48
Chapter 3.3.3 --- Paired-end RNA-Seq, dataset and reads processing --- p.48
Chapter 3.3.4 --- Genome reference and annotation --- p.49
Chapter 3.3.5 --- Strategies for reads mapping --- p.49
Chapter 3.3.6 --- Pathway and Gene Ontology analysis --- p.50
Chapter 3.3.7 --- Differential gene expression analysis --- p.50
Chapter 3.3.8 --- Ethnic specific splice junction --- p.51
Chapter 3.3.9 --- Junction sites saturation analysis --- p.51
Chapter 3.3.10 --- Ethnical novel transcribed regions --- p.52
Chapter 3.3.11 --- Isoform dynamics and meta-analysis --- p.53
Chapter 3.4 --- Result --- p.54
Chapter 3.4.1 --- Paired-end RNA-Seq --- p.54
Chapter 3.4.2 --- Differential gene expression and meta-analysis --- p.56
Chapter 3.4.3 --- Ethnic specific splice junction is rare --- p.58
Chapter 3.4.4 --- Saturation of discovery of highly confident annotated junctions --- p.59
Chapter 3.4.5 --- Novel transcribed regions --- p.62
Chapter 3.4.6 --- Isoform dynamics and meta-analysis --- p.63
Chapter 3.5 --- Discussion --- p.66
Chapter 3.6 --- Limitations --- p.67
Chapter 3.6.1 --- HapMap LCLs may not reflect the entire spectrum of natural variation --- p.67
Chapter 3.6.2 --- Sequencing depth and the usefulness of published dataset --- p.67
Chapter 3.6.3 --- Knowledge gap in understanding of the human genome --- p.69
Chapter Chapter 4: --- Transcriptomic investigation of complex disease: Type 2 Diabetes --- p.70
Chapter 4.1 --- Abstract --- p.70
Chapter 4.2 --- Introduction --- p.72
Chapter 4.3 --- Materials and Methods --- p.75
Chapter 4.3.1 --- Subjects --- p.75
Chapter 4.3.2 --- Strand-specific RNA-Seq Library Construction --- p.77
Chapter 4.3.3 --- Genome annotation sequencing reads processing --- p.81
Chapter 4.3.4 --- Reads mapping for expression analysis --- p.82
Chapter 4.3.5 --- Differential Gene expression analysis --- p.82
Chapter 4.3.6 --- GWAS candidate genes --- p.83
Chapter 4.3.7 --- Individual network, pathway and Gene Ontology analysis --- p.83
Chapter 4.3.8 --- Alternative Splicing Variation --- p.83
Chapter 4.3.9 --- Reads mapping and processing for expressed genomic variants discovery --- p.84
Chapter 4.3.10 --- Expressed and functional genomic variants --- p.85
Chapter 4.3.11 --- Screening for gene fusion --- p.86
Chapter 4.3.12 --- Sense and Antisense analysis --- p.86
Chapter 4.3.13 --- Integrated multi-level T2DM alternations gene interaction network --- p.87
Chapter 4.3.14 --- Validation of selected genes --- p.87
Chapter 4.4 --- Results --- p.88
Chapter 4.4.1 --- High quality strand-specific pair-ended RNA-Seq facilitated downstream analyses --- p.88
Chapter 4.4.2 --- Definition of significance --- p.91
Chapter 4.4.3 --- Wide-spread repressed gene expression in T2DM --- p.91
Chapter 4.4.4 --- Confirmation and curation of T2DM GWAS loci by RNA-Seq --- p.92
Chapter 4.4.5 --- Global expression alteration on T2DM associated genes --- p.97
Chapter 4.4.6 --- Alteration of relative splicing isoforms variations and T2DM specific isoforms --- p.100
Chapter 4.4.7 --- Rare and deleterious SNPs --- p.100
Chapter 4.4.8 --- Absence of alteration in Sense/Antisense ratio and expressed fusion gene --- p.101
Chapter 4.4.9 --- T2DM manifests a broad spectrum of expressed abnormalities --- p.101
Chapter 4.4.10 --- Pathway-based integration of multiple levels of alteration expanded the T2DM network --- p.103
Chapter 4.4.11 --- Validation of selected genes --- p.107
Chapter 4.5 --- Discussion --- p.108
Chapter Chapter 5: --- Conclusions and future perspectives --- p.115
Chapter 5.1 --- Conclusions --- p.115
Chapter 5.2 --- Future perspective --- p.115
Chapter 5.2.1 --- Splicing detection --- p.115
Chapter 5.2.2 --- Studies related to ethnicity --- p.116
Chapter 5.2.3 --- Complex diseases --- p.116
References --- p.118
Appendix --- p.131
"Characterisation of pathological changes in the pancreas and kidneys in type 2 diabetes mellitus." 2002. http://library.cuhk.edu.hk/record=b6073486.
Full text"June 2002."
Thesis (Ph.D.)--Chinese University of Hong Kong, 2002.
Includes bibliographical references (p. 192-210).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Mode of access: World Wide Web.
Abstracts in English and Chinese.
Harrington, Patricia Margaret. "Incident diabetes associated with second-generation antipsychotic therapy : an evaluation of the impact of dose and treatment indication." 2006. http://hdl.handle.net/2152/12997.
Full textCaleb, JoNise. "Health Communication, Health Literacy, and the Prevalence of Obesity, Depression, Anxiety and Good Disease Self-Management Among Diverse Adults Living With Type 2 Diabetes: Identifying Predictors of High Quality Patient-Provider Communication and Quality of Life." Thesis, 2021. https://doi.org/10.7916/d8-knn9-qs40.
Full text"Interaction of genetic and/ or environmental factors with maternal diabetes in increasing the susceptibility to neural tube defects." 2002. http://library.cuhk.edu.hk/record=b5891355.
Full textThesis (M.Phil.)--Chinese University of Hong Kong, 2002.
Includes bibliographical references (leaves 139-172).
Abstracts in English and Chinese.
Title page --- p.i
Acknowledgements --- p.ii
Table of Content --- p.iv
List of Figures --- p.viii
List of Graphs --- p.x
List of Tables --- p.xi
Abbreviations --- p.xiv
Abstract --- p.xv
Chinese Abstract --- p.xvii
Chapter Chapter 1 --- General Introduction --- p.1
Chapter 1.1 --- Diabetes Mellitus --- p.2
Chapter 1.1.1 --- Type 1 diabetes mellitus --- p.3
Chapter 1.1.2 --- Type 2 diabetes mellitus --- p.5
Chapter 1.1.3 --- Maturity onset diabetes of the young (MODY) --- p.6
Chapter 1.1.4 --- Gestational diabetes --- p.7
Chapter 1.2 --- Effect of Diabetes on Pregnancy --- p.9
Chapter 1.3 --- Suggested Causes of Diabetic Embryopathy --- p.10
Chapter 1.3.1 --- Glucose --- p.10
Chapter 1.3.2 --- Ketone bodies --- p.11
Chapter 1.3.3 --- Somatomedin inhibitors --- p.12
Chapter 1.3.4 --- TNF-α --- p.12
Chapter 1.3.5 --- Oxidative stress --- p.13
Chapter 1.4 --- Animal Model of Diabetes --- p.15
Chapter 1.4.1 --- Chemically-induced --- p.15
Chapter 1.4.2 --- Mutants --- p.17
Chapter 1.5 --- Gene-teratogen Interaction under Diabetic Pregnancy --- p.19
Chapter 1.6 --- Strategy of the Thesis --- p.21
Chapter Chapter 2 --- General Materials and Methods --- p.24
Chapter 2.1 --- Mouse Maintenance and Mating Method --- p.25
Chapter 2.2 --- Induction of Diabetes --- p.25
Chapter 2.3 --- Preparation of All-trans Retinoic Acid --- p.26
Chapter 2.4 --- Dissection of Embryos --- p.26
Chapter 2.5 --- DNA Extraction from Yolk Sac for Genotyping --- p.27
Chapter 2.6 --- Genotyping of Embryos --- p.28
Chapter 2.7 --- Preparation of RNA Probes for In Situ Hybridization --- p.29
Chapter 2.7.1 --- Mini-scale preparation of plasmid DNA --- p.29
Chapter 2.7.2 --- Linearization of plasmid DNA --- p.30
Chapter 2.7.3 --- In vitro transcription --- p.31
Chapter 2.8 --- Whole Mount In Situ Hybridization --- p.33
Chapter 2.8.1 --- Fixation and dehydration of embryos --- p.33
Chapter 2.8.2 --- Hybridization --- p.33
Chapter 2.8.3 --- Post-hybridization wash --- p.34
Chapter 2.8.4 --- Antibody wash and color development --- p.35
Chapter 2.8.5 --- Embryo powder preparation --- p.36
Chapter 2.8.6 --- Pre-absorption of antibody --- p.35
Chapter 2.9 --- Whole Mount TUNEL Staining --- p.36
Chapter Chapter 3 --- "Maternal Diabetes, Sp2H and RA Interaction" --- p.39
Chapter 3.1 --- Introduction --- p.40
Chapter 3.1.1 --- Neural tube defects --- p.41
Chapter 3.1.2 --- Retinoic acid as environmental factor --- p.41
Chapter 3.1.3 --- Sp2H as genetic factor --- p.44
Chapter 3.1.4 --- Experimental design of this chapter --- p.46
Chapter 3.2 --- Material and Methods --- p.47
Chapter 3.2.1 --- Sp2H mice --- p.47
Chapter 3.2.2 --- Mating and RA injection protocol --- p.47
Chapter 3.2.3 --- Dissection of fetuses and analysis of neural tube development --- p.48
Chapter 3.3 --- Results --- p.49
Chapter 3.3.1 --- Maternal diabetes alone --- p.50
Chapter 3.3.2 --- Sp2H mutation alone --- p.51
Chapter 3.3.3 --- RA alone --- p.52
Chapter 3.3.4 --- Maternal diabetes and RA interaction --- p.53
Chapter 3.3.5 --- Sp2H mutation and RA interaction --- p.55
Chapter 3.3.6 --- Sp2H mutation and maternal diabetes interaction --- p.57
Chapter 3.3.7 --- "Maternal diabetes, Sp2H mutation and RA interaction" --- p.59
Chapter 3.4 --- Discussion --- p.62
Chapter 3.4.1 --- Maternal diabetes alone does not cause neural tube defects --- p.62
Chapter 3.4.2 --- RA induces neural tube defects --- p.63
Chapter 3.4.3 --- Interaction of maternal diabetes with RA in increasing the susceptibility to neural tube defects --- p.64
Chapter 3.4.4 --- Embryos with Sp2H allele show increased susceptibility to neural tube defects when triggered by maternal diabetes and RA --- p.67
Chapter Chapter 4 --- Molecular and Cellular Bases of Interaction --- p.71
Chapter 4.1 --- Introduction --- p.72
Chapter 4.1.1 --- Mechanism of diabetic embryopathy --- p.72
Chapter 4.1.2 --- Mechanism of Sp2H mutation in development of neural tube defects --- p.74
Chapter 4.1.3 --- Mechanism of RA teratogenicity --- p.75
Chapter 4.1.4 --- "Possible common pathways shared by maternal diabetes, RA and Sp2H mutation" --- p.76
Chapter 4.1.5 --- Experimental design of this chapter --- p.78
Chapter 4.2 --- Materials and Methods --- p.80
Chapter 4.2.1 --- Sample collection for studying Pax3 expression in Sp2H/+ And +/+ embryos in response to maternal diabetes or RA by whole mount in situ hybridization --- p.80
Chapter 4.2.2 --- "Sample collection for studying the level of apoptosis in response to the interaction of maternal diabetes, Sp2H mutation and RA by whole mount TUNEL staining" --- p.82
Chapter 4.3 --- Results --- p.86
Chapter 4.3.1 --- Expression levels of Pax3 mRNA detected by whole mount in situ hybridization
Chapter 4.3.1.1 --- Expression of Pax3 in Sp2H/+/- and +/+ embryos --- p.86
Chapter 4.3.1.2 --- Effect of maternal diabetes on Pax3 expression in Sp2H/+ and +/+ embryos --- p.87
Chapter 4.3.1.3 --- Effect of RA on Pax3 expression in Sp2H /+ and +/+ embryos --- p.88
Chapter 4.3.2 --- Level of apoptosis detected by whole mount TUNEL --- p.89
Chapter 4.3.2.1 --- Effect of Sp2H allele on apoptosis --- p.94
Chapter 4.3.2.2 --- Effect of maternal diabetes on apoptosis in Sp2H/+ and +/+ embryos --- p.95
Chapter 4.3.2.3 --- Effect of RA on apoptosis in Sp2H/+ and +/+ embryos --- p.96
Chapter 4.3.2.4 --- Effect of maternal diabetes and RA on apoptosis in Sp2H/+ and +/+ embryos --- p.97
Chapter 4.4 --- Discussion --- p.99
Chapter 4.4.1 --- Underexpression of Pax3 and increases in apoptosis under maternal diabetes --- p.99
Chapter 4.4.2 --- "RA does not down regulate Pαx3, but increases apoptosis" --- p.102
Chapter 4.4.3 --- Interaction of maternal diabetes and RA in increasing apoptosis --- p.104
Chapter Chapter 5 --- "Maternal Diabetes, NOD and RA Interaction" --- p.108
Chapter 5.1 --- Introduction --- p.109
Chapter 5.1.1 --- Diabetic embryopathy in NOD mice --- p.109
Chapter 5.1.2 --- Experimental design of this chapter --- p.110
Chapter 5.2 --- Materials and Methods --- p.112
Chapter 5.2.1 --- NOD mice --- p.112
Chapter 5.2.2 --- Mating and RA Injection Protocol --- p.112
Chapter 5.2.3 --- Sample Collection for the Study of Pax3 Expression --- p.113
Chapter 5.3 --- Results --- p.115
Chapter 5.3.1 --- Maternal diabetic alone --- p.116
Chapter 5.3.2 --- NOD mutation alone --- p.117
Chapter 5.3.3 --- RA alone --- p.118
Chapter 5.3.4 --- Maternal diabetes and RA interaction --- p.119
Chapter 5.3.5 --- NOD mutation and RA interaction --- p.121
Chapter 5.3.6 --- NOD mutation and maternal diabetes interaction --- p.123
Chapter 5.3.7 --- "Maternal diabetes, NOD mutation and RA interaction" --- p.125
Chapter 5.3.8 --- Expression of Pax3 in embryos with different copies of NOD alleles --- p.128
Chapter 5.4 --- Discussion --- p.130
Chapter 5.4.1 --- Maternal diabetes interacts with NOD mutation to increase susceptibility to neural tube defects --- p.130
Chapter 5.4.2 --- Interaction of maternal diabetes with NOD mutation is greatly exacerbated when exposed to RA --- p.131
Chapter 5.4.3 --- Pax3 is not involved in the interaction --- p.133
Chapter Chapter 6 --- Conclusion and Future Perspectives --- p.134
References --- p.139
Figures
Graphs
Duncan, Henry J. (Henry John). "An isotope washout technique to study skin perfusion pressure and vascular resistance in diabetes, hypertension and peripheral vascular disease." 1986. http://web4.library.adelaide.edu.au/theses/09MD/09mdd911.pdf.
Full text"An investigation of the relationship between atherosclerosis and its risk factors amongst subjects with difference degrees of glycaemic control." Thesis, 2005. http://library.cuhk.edu.hk/record=b6073991.
Full textAtherosclerosis is the process by which the inner lining of a large or medium artery is deposited with lipids, cellular waste and other substances. It reduces the vessel's elasticity, lumen size and blood flow. Atherosclerosis is the primary underlying mechanism leading to cardiovascular and cerebrovascular diseases, the second and third leading causes of death in Hong Kong.
Both traditional and emerging risk factors for atherosclerosis were studied: traditional risk factors include age, blood pressure, indices of glycaemia control (fasting glucose, insulin and haemoglobin-Alc), and fasting lipids, while the emerging risk factors include, abdominal fat volume (subcutaneous and visceral), inflammatory markers (interleukin-6 (IL-6), interleukin-8 (IL-8) and high sensitivity c-reactive protein (hsCRP)), adiponectin, stress hormones (24 hr urinary noradrenaline and adrenaline, and plasma cortisol), and occupational stress (measured by a effort-reward imbalance questionnaire).
Starting with 204 subjects recruited from three different studies, data from 84 normoglycaemic subjects, 23 patients with impaired glucose tolerance (IGT) and 77 patients with diabetes mellitus (DM) were included in the analysis. When the IMT of the common carotid arteries and various risk factors were compared between normoglycaemic, IGT and DM subjects: (1) the IMT of the common carotid arteries showed an increasing trend with the worsening of glycaemia control (normal<IGT<DM), (2) increased prevalence of hypertension, dyslipidaemia, and obesity were observed among DM patients, and (3) increased levels of inflammatory markers, reduced concentration of adiponectin (a anti-inflammatory substance), and increased plasma cortisol concentration were also found among DM subjects. As the studies were limited by sample size, only a few risk factors were found significantly related to the carotid IMT. Age was the only common risk factor which was found to be correlated to the IMT of both the normoglycaemic and the DM/IGT subjects. (Abstract shortened by UMI.)
Fok Siu Pong.
"June 2005."
Adviser: Lester A. H. Critchley.
Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0173.
Thesis (Ph.D.)--Chinese University of Hong Kong, 2005.
Includes bibliographical references (p. 200-228).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstracts in English and Chinese.
School code: 1307.
"Effects of retinoic acid and maternal diabetes on embryonic development of caudal regression syndrome." 2000. http://library.cuhk.edu.hk/record=b6073287.
Full text"September 2000."
Thesis (Ph.D.)--Chinese University of Hong Kong, 2000.
Includes bibliographical references (p. 137-156).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Mode of access: World Wide Web.
Abstracts in English and Chinese.
Sellers, Elizabeth A. C. "The emerging epidemic of type 2 diabetes mellitus in First Nation children and youth, issues related to diagnosis, etiology, complications and treatment." 2000. http://hdl.handle.net/1993/1784.
Full textDuncan, Henry J. (Henry John). "An isotope washout technique to study skin perfusion pressure and vascular resistance in diabetes, hypertension and peripheral vascular disease / by Henry J. Duncan." Thesis, 1986. http://hdl.handle.net/2440/38294.
Full text