Journal articles: 'DG15'

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BEUCHAT, L. R., DAVID A. MANN, and JOSHUA B. GURTLER. "Comparison of Dry Sheet Media and Conventional Agar Media Methods for Enumerating Yeasts and Molds in Food." Journal of Food Protection 70, no. 11 (November 1, 2007): 2661–64. http://dx.doi.org/10.4315/0362-028x-70.11.2661.

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A study was done to compare Nissui Compact Dry Yeast and Mold plates (CDYM), 3M Petrifilm Yeast and Mold count plates (PYM), dichloran–rose bengal chloramphenicol (DRBC) agar, and dichloran 18% glycerol (DG18) agar for enumerating yeasts and molds naturally occurring in 97 foods (grains, legumes, raw fruits and vegetables, nuts, dairy products, meats, and miscellaneous processed foods and dry mixes). Correlation coefficients for plates incubated for 5 days were DG18 versus DRBC (0.93), PYM versus DRBC (0.81), CDYM versus DG18 (0.81), PYM versus DG18 (0.80), CDYM versus DRBC (0.79), and CDYM versus PYM (0.75). The number of yeasts and molds recovered from a group of foods (n = 32) analyzed on a weight basis (CFU per gram) was not significantly different (α= 0.05) when samples were plated on DRBC, DG18, PYM, or CDYM. However, the order of recovery from foods (n = 65) in a group analyzed on a unit or piece basis, or a composite of both groups (n = 97), was DRBC > DG18 = CDYM > PYM. Compared with PYM, CDYM recovered equivalent, significantly higher (α= 0.05) or significantly lower (α= 0.05) numbers of yeasts and molds in 51.5, 27.8, and 20.6%, respectively, of the 97 foods tested; respective values were 68.8, 15.6, and 15.6% in the small group (n = 32) and 43.1, 33.8, and 23.1% in the large group (n = 65) of foods. The two groups contained different types of foods, the latter consisting largely (73.8%) of raw fruits (n = 16) and vegetables (n = 32). Differences in efficacy of the four methods in recovering yeasts and molds from foods in the two groups are attributed in part to differences in genera and predominant mycoflora. While DG18 agar, CDYM, and PYM appear to be acceptable for enumerating yeasts and molds in the foods analyzed in this study, overall, DRBC agar recovered higher numbers from the 97 test foods, thereby supporting its recommended use as a general purpose medium for mycological analysis.

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Kozhina, Ekaterina A., Elizaveta S. Ershova, Natalya A. Okorokova, Vladimir P. Veiko, Elena M. Malinovskaya, Vasilina A. Sergeeva, Marina S. Konkova, Serguey I. Kutsev, Nataly N. Veiko, and Svetlana V. Kostyuk. "Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed." Oxidative Medicine and Cellular Longevity 2019 (November 3, 2019): 1–16. http://dx.doi.org/10.1155/2019/7853492.

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Background. Oxidized human DNA or plasmid DNAs containing human ribosomal genes can easily penetrate into the breast cancer cells MCF7 and stimulate the adaptive response induction. Plasmid DNA containing a CMV promoter, gene EGFP, and the insertion of the human ribosomal genes can be expressed. A hypothesis is proposed: these features of the ribosomal DNA are due to the presence of dGn motifs that are prone to oxidize. Methods. Cells of MCF7 line were cultured with plasmids which contained a CMV promoter and gene of fluorescent protein EGFP. Genetic construction pEGFP-Gn contains pEGFP vector and a small insertion with dG11 and dG13 motifs that are inclined to oxidation. The accumulation of pEGFP and pEGFP-Gn in MCF7 (qPCR), the levels of ROS in the cells, the content of 8-oxodG in plasmids and cellular DNA (flow cytometry, immunoassay, and fluorescent microscopy), the expression of NOX4 and EGFP, the localization of NOX4 and EGFP in MCF7 (qPCR, flow cytometry, and fluorescent microscopy), and the levels of the cell DNA damage (comet assay) were analyzed. Results. (dG)n insertions in the plasmid pEGFP increase the levels of ROS, the cell DNA oxidation and DNA damage, and the level of transfection of plasmid into the MCF7 cells. NOX4 participates in the oxidation of pEGFP-Gn and pEGFP. The expression of EGFP gene in MCF7 is significantly increased in case of pEGFP-Gn. Stimulation of ROS synthesis (H2O2 40 μM or 10 cGy IR) increases the level of expression of EGFP. Conclusions. GC-rich DNA fragments containing dGn motifs that are inclined to oxidation penetrate into MCF7 cancer cells, stimulate the adaptive response, and can be expressed. This property of GC-rich cell-free DNA should be considered and/or could potentially be used in therapy of tumors.

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TANIWAKI, MARTA H., NEUSELY da SILVA, ANDRÉIA A. BANHE, and BEATRIZ T. IAMANAKA. "Comparison of Culture Media, Simplate, and Petrifilm for Enumeration of Yeasts and Molds in Food." Journal of Food Protection 64, no. 10 (October 1, 2001): 1592–96. http://dx.doi.org/10.4315/0362-028x-64.10.1592.

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The efficacy of three culture media, dichloran rose bengal chloramphenicol (DRBC), dichloran 18% glycerol agar (DG18), and potato dextrose agar (PDA) supplemented with two antibiotics, were compared with the Simplate and Petrifilm techniques for mold and yeast enumeration. The following foods were analyzed: corn meal, wheat flour, cassava flour, bread crumbs, whole meal, sliced bread, ground peanuts, mozzarella cheese, grated parmesan cheese, cheese rolls, orange juice, pineapple pulp, pineapple cake, and mushroom in conserve. Correlation coefficients of DRBC versus PDA and DG18 for recovering total mold and yeast counts from the composite of 14 foods indicated that the three media were generally equivalent. Correlation coefficients for Petrifilm versus culture media were acceptable, although not as good as between culture media. Correlation coefficients of Simplate versus DRBC, DG18, PDA, and Petrifilm for recovering total yeasts and molds from a composite of 11 foods demonstrated that there was no equivalence between the counts obtained by Simplate and other culture media and Petrifilm, with significant differences observed for the most foods analyzed.

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Engel, Tobias G. P., Marlou Tehupeiory-Kooreman, Willem J. G. Melchers, Monique H. Reijers, Peter Merkus, and Paul E. Verweij. "Evaluation of a New Culture Protocol for Enhancing Fungal Detection Rates in Respiratory Samples of Cystic Fibrosis Patients." Journal of Fungi 6, no. 2 (June 9, 2020): 82. http://dx.doi.org/10.3390/jof6020082.

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Cystic fibrosis (CF) can be complicated by fungal infection of the respiratory tract. Fungal detection rates in CF sputa are highly dependent on the culture protocol and incubation conditions and thus may lead to an underestimation of the true prevalence of fungal colonization. We conducted a prospective study to evaluate the additional value of mucolytic pre-treatment, increased inoculum (100 μL), additional fungal culture media (Sabouraud agar; SAB, Medium B+, Scedosporium selective agar; SceSel+ and Dichloran-Glycerol agar; DG18) and longer incubation time (3 weeks) compared with our current protocol. Using the new protocol, we prospectively analyzed 216 expectorated sputum samples from adult and pediatric CF patients (n = 77) and compared the culture yield to a three year retrospective cohort that used direct 10 μL loop inoculation on SAB with 5 days incubation (867 sputum samples/103 patients). Detection rates for molds increased from 42% to 76% (p < 0.0001). Twenty-six percent of cultures were polymicrobial in the prospective cohort as opposed to 4.7% in the retrospective cohort (p < 0.0001). Colonization rate with A. fumigatus increased from 36% to 57%. SAB and DG18 showed the highest detection rates for all molds (SAB 58.6%; DG18 56.9%) and DG18 had the best performance for molds other than A. fumigatus. The larger sample volume and longer incubation also contributed to the increased recovery of molds. The introduction of a modified fungal culture protocol leads to a major increase in detection rate and the diversity of molds, which influences fungal epidemiology and may have implications for treatment decisions.

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Driscoll, D. M., and J. G. Williams. "Two divergently transcribed genes of Dictyostelium discoideum are cyclic AMP-inducible and coregulated during development." Molecular and Cellular Biology 7, no. 12 (December 1987): 4482–89. http://dx.doi.org/10.1128/mcb.7.12.4482.

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The cysteine proteinase 1 (CP1) gene of Dictyostelium discoideum encodes a developmentally regulated sulfhydryl proteinase. We characterized the DNA sequences upstream of the CP1 gene and found a second developmentally regulated gene, which we term DG17. The translational open reading frame of the DG17 gene encoded a 458-amino-acid cysteine- and lysine-rich protein of unknown function. In several regions, the cysteine and lysine residues were arranged in a manner characteristic of the zinc-binding domains found in proteins which interact with nucleic acids. During normal development, the DG17 and CP1 genes are coordinately activated late in aggregation. The addition of exogenous cyclic AMP (cAMP) induced the premature expression of both mRNAs. By measuring the rate of specific mRNA synthesis in isolated nuclei, we showed that cAMP acted at the transcriptional level to activate both genes. The two genes were separated by 910 nucleotides and were divergently transcribed. The intergenic region was predominantly composed of A + T residues except for four short G-rich regions. These sequences coincided with the positions of four nuclease-hypersensitive sites, which appear during aggregation when the DG17 and CP1 genes are transcribed (J. Pavlovic, E. Banz, and R. W. Parish, Nucleic Acids Res. 14:8703-8722, 1986). Two of the G-rich regions formed the core of two almost identical 80-nucleotide repeats located 220 and 320 nucleotides upstream of the CP1 gene. Using the Dictyostelium transformation system, we showed that a restriction fragment containing the intergenic region was capable of directing bidirectional transcription in a cAMP-dependent manner.

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Driscoll, D. M., and J. G. Williams. "Two divergently transcribed genes of Dictyostelium discoideum are cyclic AMP-inducible and coregulated during development." Molecular and Cellular Biology 7, no. 12 (December 1987): 4482–89. http://dx.doi.org/10.1128/mcb.7.12.4482-4489.1987.

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The cysteine proteinase 1 (CP1) gene of Dictyostelium discoideum encodes a developmentally regulated sulfhydryl proteinase. We characterized the DNA sequences upstream of the CP1 gene and found a second developmentally regulated gene, which we term DG17. The translational open reading frame of the DG17 gene encoded a 458-amino-acid cysteine- and lysine-rich protein of unknown function. In several regions, the cysteine and lysine residues were arranged in a manner characteristic of the zinc-binding domains found in proteins which interact with nucleic acids. During normal development, the DG17 and CP1 genes are coordinately activated late in aggregation. The addition of exogenous cyclic AMP (cAMP) induced the premature expression of both mRNAs. By measuring the rate of specific mRNA synthesis in isolated nuclei, we showed that cAMP acted at the transcriptional level to activate both genes. The two genes were separated by 910 nucleotides and were divergently transcribed. The intergenic region was predominantly composed of A + T residues except for four short G-rich regions. These sequences coincided with the positions of four nuclease-hypersensitive sites, which appear during aggregation when the DG17 and CP1 genes are transcribed (J. Pavlovic, E. Banz, and R. W. Parish, Nucleic Acids Res. 14:8703-8722, 1986). Two of the G-rich regions formed the core of two almost identical 80-nucleotide repeats located 220 and 320 nucleotides upstream of the CP1 gene. Using the Dictyostelium transformation system, we showed that a restriction fragment containing the intergenic region was capable of directing bidirectional transcription in a cAMP-dependent manner.

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Le, Thi Nhi Cong, Thi Ngoc Mai Cung, Thi Thanh Vu, Ngoc Minh Nghiem, Phuong Ha Hoang, Thi Lien Do, and Thi To Uyen Do. "Pyrene degradation of biofilm-forming Paracoccus sp. DG25 isolated from oil polluted samples collected in petroleum storage Duc Giang, Hanoi." Journal of Vietnamese Environment 6, no. 2 (November 5, 2014): 178–83. http://dx.doi.org/10.13141/jve.vol6.no2.pp178-183.

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In this study, a well biofilm-forming bacterial strain was isolated from oil contaminated water and sediment samples collected in petroleum storage Duc Giang, Hanoi. It was identified as Paracoccus sp. DG25 and registered in the GenBank database with the accession numbers KJ608354. Several biophysical and bio-chemical conditions for the biofilm formation of the strain were estimated such as pH, temperature, carbon sources and nitrogen sources. As the results the biofilm forming capacity was highest at pH 7, 37 oC, on maltose and supplemented with KNO3. Using these optimal conditions, the formed biofilm degraded 76.07 % of pyrene after 7 day-incubation, with the initial concentration of 300 ppm by high-performance liquid chromatography (HPLC) analysis. To our knowledge, there is rare publication on pyrene degradation by biofilm-forming bacteria. Therefore, the obtained results show that biofilm formed the strain Paracoccus sp. DG25 may considerably increase the degrading efficiency of pyrene and may lead to a new approach to treat polycyclic aromatic hydrocarbons containing in petroleum oil contaminated water in Vietnam. Trong nghiên cứu này, từ các mẫu đất và nước nhiễm dầu lấy tại kho xăng Đức Giang, Hà Nội, chúng tôi đã phân lập được chủng vi khuẩn có khả năng tạo màng sinh học tốt. Chủng vi khuẩn này đã được phân loại và định tên là Paracoccus sp. DG25 với số đăng ký trên ngân hàng Gen là KJ608354. Chúng tôi cũng đã nghiên cứu một số điều kiện hóa lý ảnh hưởng tới khả năng hình thành màng sinh học như pH, nhiệt độ, nguồn Carbon và nguồn Nitơ. Kết quả cho thấy, chủng DG25 có khả năng tạo màng tốt nhất ở các điều kiện pH 7, 37 oC, nguồn Carbon là maltose và nguồn Nitơ là KNO3. Sử dụng các điều kiện tối ưu này để tạo màng và đánh giá khả năng phân hủy pyrene của màng tạo thành. Bằng phương pháp sắc ký lỏng cao áp, chúng tôi đã đánh giá được hàm lượng pyrene bị phân hủy sau 7 ngày nuôi tĩnh bởi màng sinh học của chủng DG25 lên tới 76,07 % với nồng độ ban đầu là 300 ppm. Cho tới nay, chưa có nhiều công bố về hiệu quả phân hủy pyrene của các chủng vi khuẩn tạo màng sinh học. Do vậy, kết quả đạt được này mở ra khả năng sử dụng màng tạo thành bởi chủng DG25 để nâng cao hiệu quả phân hủy pyren và có thể mở ra phương pháp mới nhằm xử lý các hợp chất hydrocarbon thơm có trong nước ô nhiễm dầu ở Việt Nam.

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Maier, Sabine, Maja Santak, Anja Mantik, Kristina Grabusic, Elisabeth Kremmer, Wolfgang Hammerschmidt, and Bettina Kempkes. "A Somatic Knockout of CBF1 in a Human B-Cell Line Reveals that Induction of CD21 and CCR7 by EBNA-2 Is Strictly CBF1 Dependent and that Downregulation of Immunoglobulin M Is Partially CBF1 Independent." Journal of Virology 79, no. 14 (July 2005): 8784–92. http://dx.doi.org/10.1128/jvi.79.14.8784-8792.2005.

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ABSTRACT CBF1 is a cellular highly conserved DNA binding factor that is ubiquitously expressed in all tissues and acts as a repressor of cellular genes. In Epstein-Barr virus growth-transformed B-cell lines, CBF1 serves as a central DNA adaptor molecule for several viral proteins, including the viral transactivator Epstein-Barr virus nuclear antigen 2 (EBNA-2). EBNA-2 binds to CBF1 and thereby gains access to regulatory regions of target genes and activates transcription. We have inactivated the CBF1 gene by homologous recombination in the human B-cell line DG75 and characterized changes in cellular gene expression patterns upon loss of CBF1 and activation of EBNA-2. CBF1-negative DG75 cells were viable and proliferated at wild-type rates. Loss of CBF1 was not sufficient to release repression of the previously described EBNA-2 target genes CD21 or CCR7, whereas induction of both target genes by EBNA-2 required CBF1. In contrast, repression of immunoglobulin M by EBNA-2 was mainly CBF1 independent. CBF1-negative DG75 B cells thus provide an excellent tool to dissect CBF1-dependent and -independent functions exerted by the EBNA-2 protein in future studies.

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BEUCHAT, LARRY R., and DAVID A. MANN. "Comparison of New and Traditional Culture-Dependent Media for Enumerating Foodborne Yeasts and Molds." Journal of Food Protection 79, no. 1 (January 1, 2016): 95–111. http://dx.doi.org/10.4315/0362-028x.jfp-15-357.

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ABSTRACTFifty-six foods and food ingredients were analyzed for populations of naturally occurring yeasts and molds using Petrifilm rapid yeast and mold (RYM) count plates, Petrifilm yeast and mold (YM) count plates, dichloran rose bengal chloramphenicol (DRBC) agar plates, acidified potato dextrose agar (APDA) plates, and dichloran 18% glycerol (DG18) agar plates. Colonies were counted after incubating plates for 48, 72, and 120 h at 25°C. Of 56 foods in which either yeasts or molds were detected on at least one medium incubated for 120 h, neither yeasts nor molds were detected in 55.4, 73.2, 21.4, 19.6, and 71.4% of foods plated on the five respective media and incubated for 48 h; 10.7, 14.3, 3.6, 1.8, and 19.6% of foods were negative after 72 h, and 3.6, 1.8, 0, 0, and 0% of foods were negative after 120 h. Considering all enumeration media, correlation coefficients were 0.03 to 0.97 at 48 h of incubation; these values increased to 0.75 to 0.99 at 120 h. Coefficients of variation for total yeasts and molds were as high as 30.0, 30.8, and 27.2% at 48, 72, and 120 h, respectively. The general order of performance was DRBC = APDA > RYM Petrifilm > YM Petrifilm ≥ DG18 when plates were incubated for 48 h, DRBC > APDA > RYM Petrifilm > YM Petrifilm ≥ DG18 when plates were incubated for 72 h, and DRBC > APDA > RYM Petrifilm =YM Petrifilm > DG18 when plates were incubated for 120 h. Differences in performance among media are attributed to the diversity of yeasts and molds likely to be present in test foods and differences in nutrient, pH, and water activity requirements for resuscitation of stressed cells and colony development.

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TRONCHINI, ELEANDRO A., ALINE R. TREVIZAN, CRISTIANO M. TASHIMA, PRISCILA DE FREITAS, ROBERTO B. BAZOTTE, MARLI A. S. PEREIRA, and JACQUELINE N. ZANONI. "Effect of l-glutamine on myenteric neuron and of the mucous of the ileum of diabetic rats." Anais da Academia Brasileira de Ciências 85, no. 3 (September 2013): 1165–76. http://dx.doi.org/10.1590/s0001-37652013005000052.

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The objective of this work was to investigate the effect of the L-glutamine supplementation to prevent - diabetes induced changes in myenteric neurons and also to verify the effect on the mucosa of the ileum of Wistar rats. The animals were divided in five groups (n = 5): untreated normoglycaemic (UN), normoglycaemic treated with L-glutamine (NG), untreated diabetics (UD), diabetics treated with L-glutamine, starting on the 4th (DG4) or 45th day following diabetes induction (DG45). The amino acid was added to the diet at 1%. The density and size of neurons, the metaphasic index in the crypt, the height of the villus, the depth of the crypt and the number of globet cells were determined. There was no difference in the neuronal density and in the cellular body area of the myosin-stained myenteric neurons of groups DG4 and DG45 when compared to group D. The metaphase index and the number of goblet cells showed no significant differences when all groups were compared (P > 0.05). The villi height of groups DG4 and DG45 were 45.5% (P < 0.05) and 32.4% (P > 0.05) higher than those in group UD, respectively. The analyzed crypts showed similar depth for all studied groups.

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Turvill, James, Daniel Turnock, Hayden Holmes, Alison Jones, Eleanor Mclaughlan, Victoria Hilton, and Stacey Marriott. "Evaluation of the clinical and cost-effectiveness of the York Faecal Calprotectin Care Pathway." Frontline Gastroenterology 9, no. 4 (June 7, 2018): 285–94. http://dx.doi.org/10.1136/flgastro-2018-100962.

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ObjectiveTo evaluate the sensitivity and specificity of the York Faecal Calprotectin Care Pathway (YFCCP) and undertake a health economics analysis. The YFCCP has been introduced in support of the National Institute for Health and Care Excellence (NICE) guidance DG11. It is designed to improve the sensitivity and specificity of faecal calprotectin (FC) in discriminating the irritable bowel syndrome from inflammatory bowel disease in primary care.DesignTo prospectively evaluate the clinical outcomes at 6 months of the first 1005 patients entering the YFCCP. To develop a cost-consequence model using two comparators: one based on clinical assessment and the C reactive protein/erythrocyte sedimentation rate without using FC, and the second using single testing of the standard FC cut-off.SettingNorth Yorkshire primary care practices.PatientsPrimary care patients fulfilling NICE DG11.InterventionsThe YFCCP.Main outcome measuresClinical outcome measures from secondary care records.ResultsThe sensitivity and specificity of the YFCCP are 0.94 (0.85 to 0.98) and 0.92 (0.90 to 0.94), giving a negative and positive predictive value of 0.99 (0.98 to 1.0) and 0.51 (0.43 to 0.59), respectively.ConclusionsThe YFCCP overcomes the challenges experienced with FC use in primary care, its efficacy matching initial NICE projections. It is readily incorporated into clinical practice. It should represent the framework on which to increase NICE DG11 implementation nationally.

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Neofytou, Marina C., Charoulla Michael, Constantina Constantinou, Dionysis Sparaggis, and Ouranios Tzamaloukas. "Feeding wheat dried distillers’ grains with solubles increases conjugated linoleic acid and unsaturated lipids in ovine milk without adversely affecting milk yield." Journal of Dairy Research 88, no. 2 (May 2021): 128–33. http://dx.doi.org/10.1017/s0022029921000443.

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AbstractThe aim of this research communication was to examine the effect of dietary supplementation with wheat-based dried distillers’ grains with solubles (DDGS), a by-product of bioethanol production, on yield, composition, and fatty acid (FA) profile of ewe milk. Forty-five purebred mid-lactating Chios ewes (average milk yield 2.23 kg/d in 96 ± 5 d in lactation) were offered three iso-nitrogenous and iso-energetic diets (15 animals per diet) for a 10 d adaptation period followed by a 5-week recording and sampling period. The diets contained 0, 6, and 12% DDGS on DM basis for the DG0, DG6, and DG12 treatment, respectively, as a replacement of concentrate mix, whilst concentrate-to-forage ratio remained at 60:40 in all treatments. Individual milk yield, milk composition, and FA profile were recorded weekly and analyzed using a complete randomized design with repeated measurements. No significant differences were observed among groups concerning dry matter intake (overall mean of 2.59 kg/d), milk yield or 6% fat-corrected milk and milk protein percentage or protein yield. Milk fat percentage was decreased in the DG12 (4.76%) compared to DG0 (5.69%) without, however, significantly affecting the daily output of milk fat. The concentration of all major saturated FA between C4:0 to C16:0 was reduced, whereas long-chain (>16 carbons), mono-unsaturated and poly-unsaturated FAs were increased in the milk of DDGS groups. Among individual FA, increments of oleic acid and C18:1 trans-monoenes like C18:1 trans-10 and C18:1 trans-11 were demonstrated in DG12 group, whereas linoleic and conjugated linoleic acid (CLA cis-9, trans-11) were elevated in both DDGS groups compared to control. Changes in FA profile resulted in a decline in the atherogenic index of milk by 20% and 35% in DG6 and DG12 treatments, respectively, compared with control. In conclusion, feeding DDGS to dairy ewes increased the levels of unsaturated FA that are potentially beneficial for human health without adversely affecting milk, protein or fat yield.

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Lasmana, Indradhi, Ludofikus Dumin, Stefen Ndun, and Joko Suparmanto. "ANALISA KINERJA DAN PRIORITAS SISTEM DRAINASE DI KAWASAN KOTA BORONG KABUPATEN MANGGARAI TIMUR." JUTEKS - Jurnal Teknik Sipil 2, no. 1 (November 10, 2017): 64. http://dx.doi.org/10.32511/juteks.v2i1.125.

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Banjir atau genangan di suatu kawasan terjadi apabila sistem yang berfungsi untuk menampung genangan itu tidak mampu menampung debit yang mengalir, hal ini akibat dari tiga kemungkinan yang terjadi yaitu : kapasitas sistem yang menurun, debit aliran air yang meningkat, atau kombinasi dari kedua-duanya. Perkembangan kota semakin hari semakin terus berkembang dan kebutuhan masyarakat akan ruang permukiman semakin tinggi sehingga berpengaruh terhadap ruang terbuka dan resapan air di wilayah Kota Borong. Analisis kinerja saluran drainase di kawasan wilayah Kota Borong menjadi suatu langkah penting guna mencari solusi dalam penanganan masalah banjir dan genangan yang ada. Dari hasil identifikasi kondisi sistem drainase dan kawasan yang mengalami banjir dan genangan di Kota Borong Kabupaten Manggarai Timur terdapat 8 (delapan) titik yang menjadi prioritas penanganan yaitu kawasan pasar Borong, Pasar Borong/Terminal, depan Koramil Kota Borong, Jalan Srikaya (depan Masjid Kota Ndora), Jalan Srikaya (depan Kantor DPRD), Jalan Sukun (masuk dari jalan negara), Jalan Sukun (kampung bugis peji depan kios warga), Perempatan di Kampung Bugis. Hasil Analisis Kinerja sistem jaringan drainase di kawasan Kota Borong dengan berbagai kriteria diperoleh kondisi bangunan cukup. Hal ini terlihat pada persentase kondisi sistem jaringan drainase di masing-masing sub sistem, yaitu kondisi di DG1 = 63,53%, kondisi di DG2 = 66,50%, konsisi di DG3 = 69% , DG4 = 60%, DG5 = 61,25%, DG6 = 68.37%, DG7 = 53,27% dan kondisi di DG8 = 60,71%. Analisis Penentuan skala prioritas penanganan dan penentuan daerah prioritas wilayah pasar Borong mempunyai nilai yang paling tinggi yaitu 456.25, Pasar Borong/Terminal = 421.25, Jalan Srikaya 351.25, Jalan sukun 237.5, Jalan Sukun Kampung bugis Peji 237.5 dan perempatan Kampung Bugis 237.5 dengan dilakukannya penanganan meliputi peninggian badan jalan, perbaikan kapasitas saluran dan elevasi saluran serta perlunya adanya bangunan-bangunan resapan.

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Bruno, Tony F., Donald E. Woods, Douglas G. Storey, and Christopher H. Mody. "RecombinantPseudomonasexoenzyme S and exoenzyme S fromPseudomonas aeruginosaDG1 share the ability to stimulate T lymphocyte proliferation." Canadian Journal of Microbiology 45, no. 7 (August 1, 1999): 607–11. http://dx.doi.org/10.1139/w99-044.

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Exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 have distinct characteristics, which has led to a controversy about their homology and their pathophysiologic consequences. We have been investigating the ability of exoenzyme S to activate T lymphocytes, and therefore performed studies to determine whether exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 and expressed in Pseudomonas aeruginosa PA103 or in E. coli BL21(DE3), could induce T lymphocyte activation and proliferation. Both preparations were able to activate T cells and induce lymphocyte proliferation at similar levels as measured by flow cytometry of surface-activation markers and DNA synthesis, respectively. Further, a monoclonal antibody raised against exoenzyme S from strain DG1 partially neutralized T cell activation induced by recombinant exoenzyme S and bound to it in an immunoblot suggesting that the epitope responsible for T cell activation is shared by exoenzyme S from strain DG1 and recombinant exoenzyme S. These data suggest that the two different preparations of exoenzyme S, despite biochemical differences, share the characteristic that is responsible for T lymphocyte activation.Key words: exoenzyme S, Pseudomonas aeruginosa, T lymphocyte, cystic fibrosis.

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González-Pajuelo, María, Isabelle Meynial-Salles, Filipa Mendes, Philippe Soucaille, and Isabel Vasconcelos. "Microbial Conversion of Glycerol to 1,3-Propanediol: Physiological Comparison of a Natural Producer, Clostridium butyricum VPI 3266, and an Engineered Strain, Clostridium acetobutylicum DG1(pSPD5)." Applied and Environmental Microbiology 72, no. 1 (January 2006): 96–101. http://dx.doi.org/10.1128/aem.72.1.96-101.2006.

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ABSTRACT Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum DG1, growth on glycerol was achieved, and 1,3-propanediol was produced. In order to compare the physiological behavior of the recombinant C. acetobutylicum DG1(pSPD5) strain with that of the natural 1,3-propanediol producer C. butyricum VPI 3266, both strains were grown in chemostat cultures with glycerol as the sole carbon source. The same “global behavior” was observed for both strains: 1,3-propanediol was the main fermentation product, and the qH2 flux was very low. However, when looking at key intracellular enzyme levels, significant differences were observed. Firstly, the pathway for glycerol oxidation was different: C. butyricum uses a glycerol dehydrogenase and a dihydroxyacetone kinase, while C. acetobutylicum uses a glycerol kinase and a glycerol-3-phosphate dehydrogenase. Secondly, the electron flow is differentially regulated: (i) in C. butyricum VPI 3266, the in vitro hydrogenase activity is 10-fold lower than that in C. acetobutylicum DG1(pSPD5), and (ii) while the ferredoxin-NAD+ reductase activity is high and the NADH-ferredoxin reductase activity is low in C. acetobutylicum DG1(pSPD5), the reverse is observed for C. butyricum VPI 3266. Thirdly, lactate dehydrogenase activity is only detected in the C. acetobutylicum DG1(pSPD5) culture, explaining why this microorganism produces lactate.

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Schryvers, H. J., E. Kuhn, and A. Vangheluwe. "Survey on EPO — DG1 search quality." World Patent Information 16, no. 2 (June 1994): 83–89. http://dx.doi.org/10.1016/0172-2190(94)90016-7.

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Viegas, Carla, Marta Dias, Beatriz Almeida, Estela Vicente, Carla Candeias, Liliana Aranha Caetano, Elisabete Carolino, and Célia Alves. "Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe." Atmosphere 12, no. 3 (March 13, 2021): 378. http://dx.doi.org/10.3390/atmos12030378.

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Sampling campaigns indoors have shown that occupants exposed to contaminated air generally exhibit diverse health outcomes. This study intends to assess the deposition rates of total settleable dust and bioburden in the indoor air of dwellings onto quartz fiber filters and electrostatic dust collectors (EDCs), respectively. EDC extracts were inoculated onto malt extract agar (MEA) and dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy agar (TSA) was applied for total bacteria assessment, and violet red bile agar (VRBA) for Gram-negative bacteria. Azole-resistance screening and molecular detection by qPCR was also performed. Dust loading rates ranged from 0.111 to 3.52, averaging 0.675 μg cm−2 day−1. Bacterial counts ranged from undetectable to 16.3 colony-forming units (CFU) m−2 day−1 and to 2.95 CFU m−2 day−1 in TSA and VRBA, respectively. Fungal contamination ranged from 1.97 to 35.4 CFU m−2 day−1 in MEA, and from undetectable to 48.8 CFU m−2 day−1 in DG18. Penicillium sp. presented the highest prevalence in MEA media (36.2%) and Cladosporium sp. in DG18 (39.2%). It was possible to observe: (a) settleable dust loadings and fungal contamination higher in dwellings with pets; (b) fungal species considered indicators of harmful fungal contamination; (c) Aspergillus section Candidi identified in supplemented media with voriconazole and posaconazole; (d) specific housing typologies and (e) specific housing characteristics influencing the microbial contamination.

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Hua, Fei, and Hong Qi Wang. "Factors Influencing Crude Oil Biodegradation by Pseudomonas sp. DG17." Asian Journal of Chemistry 26, no. 15 (2014): 4637–42. http://dx.doi.org/10.14233/ajchem.2014.16149.

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Wang, Hong Qi, Fei Hua, Yi Cun Zhao, Yi Li, and Xuan Wang. "Immobilization ofPseudomonassp. DG17 onto sodium alginate–attapulgite–calcium carbonate." Biotechnology & Biotechnological Equipment 28, no. 5 (September 3, 2014): 834–42. http://dx.doi.org/10.1080/13102818.2014.961123.

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20

Viegas, Carla, Raquel Pimenta, Marta Dias, Bianca Gomes, Miguel Brito, Liliana Aranha Caetano, Elisabete Carolino, and Anita Quintal Gomes. "Microbiological Contamination Assessment in Higher Education Institutes." Atmosphere 12, no. 8 (August 23, 2021): 1079. http://dx.doi.org/10.3390/atmos12081079.

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The higher education sector represents a unique environment and it acts as a work environment, a learning environment for students, and frequently, also a home environment. The aim of this study was to determine the microbial contamination (SARS-CoV-2, fungi, and bacteria) in Higher Education Facilities (HEI) by using active and passive sampling methods and combining culture-based methods with molecular tools targeting Aspergillus section Fumigati. In addition, the resistance to azole profile was also assessed. Surface samples showed a range of total bacterial contamination between 1 × 103 to 3.1 × 106 CFU·m−2, while Gram-negative bacteria ranged from 0 to 1.9 × 104 CFU·m−2. Fungal contamination ranged from 2 × 103 to 1.8 × 105 CFU·m−2 on MEA, and from 5 × 103 to 1.7 × 105 CFU·m−2 on DG18. The most prevalent species found on both media was Cladosporium sp. (47.36% MEA; 32.33% DG18). Aspergillus genera was observed on MEA (3.21%) and DG18 (14.66%), but not in the supplemented media used for the azole screening. Aspergillus section Fumigati was detected in 2 air samples (2.22%, 2 out of 90 samples) by qPCR. When testing for SARS-CoV-2 all results were negative. The present study showed that although cleaning and disinfection procedures are done regularly due to the COVID-19 pandemic, being effective in eliminating SARS-CoV-2, surfaces were often contaminated with microorganisms other than SARS-CoV-2. This can be a result of increasing resistance to biocides, and to the wide range of environmental factors that can contribute to the dissemination of microbial contamination indoors.

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Kungulovski, Dzoko, Oliver Avramoski, Natalija Atanasova-Pancevska, and Ivan Kungulovski. "Mycotoxigenic molds in spices from Macedonian stores." Zbornik Matice srpske za prirodne nauke, no. 120 (2011): 155–63. http://dx.doi.org/10.2298/zmspn1120155k.

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Twenty-six samples of spices most frequently occurring in the stores of the Republic of Macedonia were examined for their fungal contamination and the incidence of Aspergillus and Penicillium species and their teleomorphs. It included mainly commercial packages most frequently occurring in the stores of the Republic of Macedonia. According to the relative frequency of each of the isolated species, the typical mycoflora of these samples includes A. niger, A. flavus, A. fumigatus, P. chrysogenum and Eurotium sp. Fungal counts varied from log10 < 2 CFU g-1 (DG18 at 25oC; DRBC at 25oC), for a sample of paprika, to log10 6.17 CFU g-1 (DG18 at 25oC), for a sample of bay leaf. During the experiment, A. flavus was detected in 17 samples, out of which 7 isolates were capable of producing Af-B1, and 4 isolates produced Af-B2. All isolates of A. nomius and A. parasiticus, in the experimental conditions, produced Af-B1, Af-B2, Af-G1 and Af-G2.

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Kraus, Richard J., Sarah J. Mirocha, Heather M. Stephany, Joel R. Puchalski, and Janet E. Mertz. "Identification of a Novel Element Involved in Regulation of the Lytic Switch BZLF1 Gene Promoter of Epstein-Barr Virus." Journal of Virology 75, no. 2 (January 15, 2001): 867–77. http://dx.doi.org/10.1128/jvi.75.2.867-877.2001.

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ABSTRACT Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5′-CAGGTA-3′, called ZV, located at nucleotides −17 to −12 relative to the transcription initiation site of the BZLF1 promoter. ZV sequence-specifically binds a cellular nuclear factor(s), ZVR. ZVR DNA-binding activity was present in the EBV-negative B-lymphocytic cell line DG75, the EBV-positive B-lymphocytic cell lines GG68 and 721, the cervical cell line C33A, and the kidney cell line CV-1 but not in the breast carcinoma cell line MCF-7. Mutations in ZV that relieve binding of ZVR lead to a two- to fourfold increase in basal expression of the BZLF1 promoter in DG75, C33A, and CV-1 cells. The same mutants exhibited a 40- to 180-fold increase in tetradecanoyl phorbol acetate-ionomycin-induced expression in DG75 cells and a 22-fold increase in C33A cells. Thus, ZVR functions as a regulator of the BZLF1 promoter, repressing transcription when bound to the ZV site in the absence of inducers. No differences in basal or induced transcription between wild-type and ZV mutant BZLF1 promoters were observed in ZVR-negative MCF-7 cells. ZVR failed to bind any of the previously identified negative regulatory elements within the BZLF1 promoter. We conclude that ZV functions as an important regulatory element of the BZLF1 promoter, with ZVR likely playing important roles in the maintenance of latency and reactivation of EBV.

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Schryvers, H. J., and E. Kuhn. "Second survey on EPO — DG1 search quality." World Patent Information 18, no. 3 (September 1996): 155–63. http://dx.doi.org/10.1016/0172-2190(96)00018-x.

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Dang, Jason, Susan Blandford, Maruse Sadek, D. Grobelny, and Robert T. C. Brownlee. "The solution structures of the HIV protease inhibitor DG35-VIII." Molecular Simulation 28, no. 8-9 (August 2002): 827–43. http://dx.doi.org/10.1080/0892702021000002511.

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Hua, Fei, Hong Qi Wang, and Yi Cun Zhao. "Factors influencing the trans-membrane transport ofn-octadecane byPseudomonassp. DG17." Biotechnology & Biotechnological Equipment 28, no. 3 (May 4, 2014): 463–70. http://dx.doi.org/10.1080/13102818.2014.923601.

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26

Hua, Fei, Hong Qi Wang, Yi Li, and Yi Cun Zhao. "Trans-membrane transport of n-octadecane by Pseudomonas sp. DG17." Journal of Microbiology 51, no. 6 (December 2013): 791–99. http://dx.doi.org/10.1007/s12275-013-3259-6.

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27

Magnoli, C., A. Astoreca, M. Ponsone, C. Barberis, M. Fernández-Juri, and A. Dalcero. "Ochratoxin- and aflatoxin-producing fungi associated with green and roasted coffee samples consumed in Argentina." World Mycotoxin Journal 1, no. 4 (November 1, 2008): 419–27. http://dx.doi.org/10.3920/wmj2008.1023.

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The aims of this work were to identify the Aspergillus sections Nigri and Flavi, and to evaluate the natural occurrence of ochratoxin A (OTA) and aflatoxins in green and roasted coffee bean samples. The capacity to produce these toxins by Aspergillus species was also studied. Fifty samples of Colombian coffee beans (25 green and 25 roasted) were obtained from a processor plant located in the south of Córdoba province (Argentina). OTA and aflatoxin analysis were performed by high performance liquid chromatography (HPLC). OTA production by strains belonging to Aspergillus niger aggregate were cultivated using YES medium and detected by HPLC. Aflatoxin production was tested in strains belonging to section Flavi on malt extract agar and was detected by thin liquid chromatography (TLC). From green coffee samples, the predominant species isolated belonged to A. niger aggregate, 60 and 55%, in dichloran rose bengal chloramphenicol agar (DRBC) and dichloran 18% glycerol agar (DG18) respectively. While A. flavus strains were isolated in 14% from DRBC and A. parasiticus strains in 12% and 28% in DRBC and DG18, respectively. From roasted coffee samples, A. flavus was the most predominant fungi, isolated in similar percentages from both media (28%); followed by A. niger aggregate isolated in 28 and 14% in DRBC and DG18, respectively. In green and roasted coffee samples mean colony counts ranged from 2×103 to 3.5×104 colony forming units per gram of sample (cfu/g). OTA and aflatoxins were not detected in any sample analyses (<1 and 0.5 ng/g for OTA and aflatoxins, respectively). Twenty-five percent of black Aspergillus strains were OTA producers. The total of A. flavus strains assayed produced aflotoxin B1 (AFB1) and 80% of the A. parasiticus strains were AFB1 and aflotoxin G1 producers. The high percentage of A. flavus and A. parasiticus aflatoxin-producing strains suggest a potential risk for contamination in coffee with aflatoxins.

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Viegas, Carla, Marta Dias, Elisabete Carolino, and Raquel Sabino. "Culture Media and Sampling Collection Method for Aspergillus spp. Assessment: Tackling the Gap between Recommendations and the Scientific Evidence." Atmosphere 12, no. 1 (December 26, 2020): 23. http://dx.doi.org/10.3390/atmos12010023.

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Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.

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QI, ZONGLI, YUAN LI, JUN HU, HUA GUO, XIANGRONG ZHAO, GUANGHUA WANG, JINWEI GAO, and QIAOXIA HU. "The DG75 B-cell lymphoma line exhibits biclonal immunoglobulin gene rearrangement." Biomedical Reports 1, no. 1 (October 12, 2012): 111–14. http://dx.doi.org/10.3892/br.2012.22.

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30

Sathiyaraj, Gayathri, Myung Kyum Kim, Ju-Young Kim, Su-Jeong Kim, Jun Hwee Jang, Soohyun Maeng, Myung-Suk Kang, and Sathiyaraj Srinivasan. "Complete genome sequence of Nibribacter radioresistens DG15C, a radiation resistant bacterium." Molecular & Cellular Toxicology 14, no. 3 (June 23, 2018): 323–28. http://dx.doi.org/10.1007/s13273-018-0035-z.

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31

Feederle, Regina, Henri-Jacques Delecluse, Jean-Pierre Rouault, Aloys Schepers, and Wolfgang Hammerschmidt. "Efficient somatic gene targeting in the lymphoid human cell line DG75." Gene 343, no. 1 (December 2004): 91–97. http://dx.doi.org/10.1016/j.gene.2004.08.005.

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32

Fallo, Gergonius. "Pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada Berbagai Media." Savana Cendana 2, no. 03 (July 25, 2017): 39–41. http://dx.doi.org/10.32938/sc.v2i03.41.

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Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri merupakan cendawan perusak bahan pangan yang ditemukan pada saat bahan pangan belum dipanen atau setelah bahan pangan dipanen. Pertumbuhan ketiga cendawan ini sangat dipengaruhi oleh nutrisi yang terkandung pada media tumbuh. Penelitian ini bertujuan untuk membandingkan pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada beberapa media untuk isolasi dan identifikasi. Ketiga cendawan tersebut ditumbuhkan pada media yang berbeda yaitu Chloramphenicol Peptone Agar (DCPA), Dichloran 18% Glycerol Agar (DG18), Czapek Yeast Extract Agar (CYA), Czapek Yeast Extract Agar 20% Sucrose (CY20S), Malt Extract Agar (MEA), dan 25% Glycerol Nitrate Agar (G25N) dan diinkubasi pada suhu + 280C. Pengamatan koloni dilakukan setiap 2 x 24 jam, 4 x 24 jam, dan 6 x 24 jam selanjutnya diameter isolat diukur. Hasil isolasi pertumbuhan dan panjang diameter koloni dari A. Flavus (61 mm) dan E. Chevalieri (45mm) diketahui dapat tumbuh baik pada media DG18, sedangkan F. verticillioides (46 mm) tumbuh baik di media DCPA. Sementara pada media identifikasi A. Flavus (80 mm) dapat tumbuh baik pada media CY20S, sedangkan E. Chevalieri (40 mm) dapat tumbuh baik pada media G25N dan CY20S. Koloni F. verticillioides (62 mm) dapat tumbuh baik di media CYA dan CY20S. ©2017 dipublikasikan oleh Savana Cendana.

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Sari, Herna Permata, Yohanes Aris Purwanto, and I. Wayan Budiastra. "Pendugaan Kandungan Kimia Mangga Gedong Gincu Menggunakan Spektroskopi Inframerah Dekat (Prediction of Chemical Contents in ‘Gedong Gincu’ Mango using Near Infrared Spectroscopy)." Jurnal Agritech 36, no. 03 (December 21, 2016): 294. http://dx.doi.org/10.22146/agritech.16599.

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The objective of this work was to predict the soluble solid content, total acid, sugar acid ratio, and crude fiber of ‘Gedong Gincu’ mango non destructive using Near infrared Spectroscopy. Experiments were carried out using 182 samples of ‘Gedong Gincu’ mango. NIR reflectance spectra measurements were performed at wavelength of 1000-2500 nm using NIRFlex N-500 fiber optic solid. References data were collected from laboratory measurements. Five pre-processing treatments, smoothing 3 points (sa3), normalization (n01), first derivative Savitzky-Golay 9 points (dg1), combination (n01, dg1), and the Multiplicative Scatter Correction (MSC) were used to improve the accuracy of the calibration model. Partial Least Square (PLS) method was used to calibrate NIR data through references data. The results show that the best method for prediction of soluble non solid spectra were MSC and 12 factor of PLS with calibration value of Correlation Coefficient (r), Square Error Calibration (SEC), Square Error Prediction (SEP), Ratio of standard error prediction to deviation (RPD) were 0.91, 0.25 %, 0.39 %, 2.14 respectively. Sugar acid ratio content was predictd using MSC and 12 factor of PLS calibration with values of r, SEC, SEP, RPD were 0.81, 32.08 °Brix/%, 38.44 °Brix/%, 1.45. Soluble solid content was predicted using sa3 and 15 factor of PLS calibration with values of r, SEC, SEP, RPD were 0.82, 1.04 °Brix, 1.28 °Brix, 1.52 respectively. Total acid was predicted using dg1 and 3 with the value of r, SEC, SEP, RPD were 0.74, 0.01 %, 0.12 %, 1.33 respectively. It could be concluded that the developed model could be used to predict the chemical contents of ‘Gedong Gincu’ mango non destructively. ABSTRAKTujuan dari penelitian ini adalah memprediksi kandungan total padatan terlarut (TPT), total asam, rasio gula asam, dan padatan tidak terlarut (serat kasar) mangga Gedong Gincu secara non destruktif menggunakan spektroskopi inframerah dekat (NIR). Bahan yang digunakan berupa mangga Gedong Gincu sebanyak 182 buah. Pengukuran spektra reflektan NIR dilakukan pada panjang gelombang 1000 – 2500 nm menggunakan NIRFlex N-500 fiber optik solid dilanjutkan pengukuran data referensi laboratorium. Lima pra-proses data spektra yaitu smoothing 3 points (sa3), normalisasi (n01), first derivative Savitzzky-golay (dg1), kombinasi (n01,dg1), dan Multiplicative Scatter Correction (MSC) dilakukan untuk meningkatkan akurasi model kalibrasi. Kalibrasi data NIR dan data kimia dilakukan menggunakan metode Partial Least Square (PLS). Metode terbaik untuk prediksi padatan tidak terlarut diperoleh dengan pra-proses MSC dan kalibrasi PLS dengan nilai Correlation Coefficient (r), Square Error Calibration (SEC), Square Error Prediction (SEP), Ratio of standard error prediction to deviation (RPD) adalah 0,91, 0,25 %, 0,39 %, 2,14, dan faktor PLS 12. Kandungan rasio gula asam diduga dengan pra-proses MSC serta kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,81, 32,08 °Brix/%, 38,44 °Brix/%, 1,45 dan faktor PLS yang digunakan 12. TPT diduga menggunakan pra-proses sa3 dan kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,82, 1,04 oBrix, 1,28 °Brix, 1,52. Model kalibrasi total asam diperoleh pra-proses dg1 dan kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,74, 0,01 %, 0,12 %, 1,33. Hasil penelitian ini menunjukkan bahwa model yang dikembangkan dapat digunakan untuk menduga kandungan kimia mangga Gedong Gincu secara non destruktif.Kata kunci: Mangga Gedong Gincu; non destruktif; partial least square; pra-proses; spektroskopi NIR

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Strom, Robert, Shirley Strom, Yuh-Ling Shen, Shing-Jing Li, and Hwey-Lin Sun. "Grandparents in Taiwan: A Three-Generational Study." International Journal of Aging and Human Development 42, no. 1 (January 1996): 1–19. http://dx.doi.org/10.2190/d7r2-dg1l-ddfy-ptm7.

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Grandparents in the Republic of China want to remain influential, but social policy has not provided them with education to fulfill their changing role. The performance of grandparents was examined to determine suitable content for an intervention program. A sample of 751 non-consanguineous participants from urban and rural Taiwan included 234 grandparents, 241 parents, and 276 grandchildren. Each generation was administered a separate version of the Grandparent Strengths and Needs Inventory that was translated into Mandarin. Respondents identified favorable qualities of grandparents as well as aspects of their relationships in which growth was necessary. Multivariate analysis of variance, univariate analysis of variance, Scheffé and t-tests were used to analyze scores, confirm results, and facilitate interpretation. All three generations described aspects of grandparent success and specific realms of learning they should acquire to become more effective. Significant main effects that influenced responses about grandparent performance were generation, gender of grandchild, age of grandchild, frequency of grandchild care by grandparent, generations living together, and amount of time grandparent and grandchild spent together. Considerations were recommended to improve behavior of grandparents and guide the development of educational programs for them.

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35

Olsen, M., K. Andersson, and K. Åkerstrand. "Quality control of two rose bengal and modified DRBC and DG18 media." International Journal of Food Microbiology 35, no. 2 (April 1997): 163–68. http://dx.doi.org/10.1016/s0168-1605(96)01215-9.

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36

Hua, F., and H. Wang. "Uptake modes of octadecane by Pseudomonas sp. DG17 and synthesis of biosurfactant." Journal of Applied Microbiology 112, no. 1 (November 22, 2011): 25–37. http://dx.doi.org/10.1111/j.1365-2672.2011.05178.x.

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37

Gargan, P. E., V. A. Ploplis, and J. D. Scheu. "A Fibrin Specific Monoclonal Antibody which Interferes with the Fibrinolytic Effect of Tissue Plasminogen Activator." Thrombosis and Haemostasis 59, no. 03 (1988): 426–31. http://dx.doi.org/10.1055/s-0038-1647509.

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SummaryMonoclonal antibodies to human fibrin have been prepared from stable hybridomas, obtained by fusion of a mouse myeloma cell line (NS-1) and spleen cells of Balb/c mice immunized with a suspension of human fibrin. One cell line, DG1, producing a monoclonal antibody of the IgG1 κ subclass, reacted specifically with human fibrin (KD = 1.2 nM). Western blotting analysis indicates that DG1 crossreacts with the fibrin fragment D-dimer. Using both a chromogenic and an 125I-fibrin release assay it was illustrated that in the presence of the fibrin specific antibody the t-PA mediated generation of plasmin was significantly inhibited.An animal model system, developed to monitor thrombosis and induced reactive fibrinolysis, was used to investigate the interference of plasminogen activation, by the antibody, in vivo.This fibrin specific antibody prolonged the onset of reactive fibrinolysis in a dose dependent manner.

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Hess, Josef. "DGK5-Feldvergleich mit dem Programm RoSy®-Hybrid-Editor und der Applikation DGK5Pen." KN - Journal of Cartography and Geographic Information 52, no. 4 (July 2002): 161–63. http://dx.doi.org/10.1007/bf03544903.

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39

Hua, Fei, Hong Qi Wang, Yi Cun Zhao, and Yan Yang. "Pseudosolubilized n-alkanes analysis and optimization of biosurfactants production by Pseudomonas sp. DG17." Environmental Science and Pollution Research 22, no. 9 (November 22, 2014): 6660–69. http://dx.doi.org/10.1007/s11356-014-3853-0.

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40

Verhoeff, A. P., J. H. Van Wijnen, P. Fischer, B. Brunekreef, J. S. M. Boleij, E. S. Van Reenen, and R. A. Samson. "Presence of Viable Mould Propagules in the Indoor Air of Houses." Toxicology and Industrial Health 6, no. 5 (October 1990): 133–45. http://dx.doi.org/10.1177/074823379000600510.

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The aim of the first port of this study was to select the optimal technique for the enumeration and identification of viable mould propagules in the indoor air of houses. A comparison was made between the results obtained with six commercially available air sampling devices in combination with four culture media. The optimal technique was defined as the technique with the best precision and the highest yield. The coefficients of variation were high (generally > 20%) for all combinations. Statistical analysis showed that the Slit sampler and the N6-Andersen sampler in combination with DG18 and MEA gave the best precision and the highest yield in terms of CFU/m3 and number of species isolated. In the second part of this study the presence of viable mould propagules in the indoor air of 46 houses in relation to the dampness of these houses was investigated, using the N6-Andersen sampler in combination with DG18. To assess the variability in time, the measurements were repeated after five weeks. Overall, between the two periods no difference was found between the average number of CFU/m3 in the investigated homes. However, the variation between homes was much smaller than the variation within homes. The mean number of CFU/m3 was somewhat higher in “damp” houses than in “dry” houses. However, this difference was not significant. Furthermore, there were no demonstrable differences in the presence of specific mould species in “damp” and “dry” houses.

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Fleming, T. P., D. R. Garrod, and A. J. Elsmore. "Desmosome biogenesis in the mouse preimplantation embryo." Development 112, no. 2 (June 1, 1991): 527–39. http://dx.doi.org/10.1242/dev.112.2.527.

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The molecular processes underlying the formation of the first desmosomes in the mouse early embryo have been examined by immunocytochemical and biochemical techniques using antibody probes recognising desmosomal proteins 1 and 2 (dp1 + 2, desmoplakins), dp3 (plakoglobin), desmosomal glycoprotein 1 (dg1, desmoglein) and dg2 + 3 (desmocollins). Immunofluorescence labelling of staged intact embryos and synchronised cell clusters indicates that dp1 + 2, dg1 and dg2 + 3 are first detectable on the lateral membrane contact sites between trophectoderm cells in early cavitating blastocysts, coincident with the onset of desmosome formation as seen in ultrastructural preparations. Membrane localisation of these antigens is predominantly punctate in appearance, occurs after division to the 32-cell stage and appears to be coincident with blastocoele formation since non-cavitated embryos/cell clusters of equivalent age/cell cycle are usually unlabelled. In contrast, dp3 is first detectable at the 32-cell stage at all internal membrane contact sites (including those with inner cell mass cells) in a continuous linear pattern, and appears in both cavitated and non-cavitated specimens. Subsequently during blastocyst expansion, dp3 localisation becomes punctate and restricted to trophectodermal membranes. Immunoprecipitation of desmosomal antigens following metabolic labelling indicates that synthesis of dp3 is underway from at least compaction in the 8-cell embryo, while dp1 + 2 synthesis is first evident in 16-cell morulae. Synthesis of dg1 and dg2 + 3 is not detectable until the early blastocyst stage. These results suggest that desmosome biogenesis in the preimplantation embryo might be regulated by transcription or translation of desmosomal glycoproteins and by maturational changes in the trophectoderm layer associated with blastocoele formation. The earlier expression and wider distribution of dp3 at cell contact areas may reflect non-desmosomal sites (eg, adherens junctions) for this protein and a possible role for dp3 in the development of intercellular junctions.

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Mirzoyan, L. V. "Some Notes on SS Cygni." International Astronomical Union Colloquium 93 (1987): 119–22. http://dx.doi.org/10.1017/s0252921100104725.

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AbstractThe results of photoelectric and spectral observations of SS Cyg are briefly discussed. Some features of the spectral variations observed during a large outburst of SS Cyg can probably be explained using a model of a double star which is surrounded by an absorbing gaseous shell. It is noted that the existing data are not numerous enough for the explanation of the SS Cyg variability, in particular, its short-term variations.SS Cyg is a spectroscopic binary star with a 0.d276 period. The components of this system are dwarf stars (M ≈ 5.m5 for both stars), with dG5 and sdBe spectra; they probably show eruptive activity (Joy, 1956). In the spectrum of the B-component strong emission lines are observed. The dG5 spectrum is observed only in the minimum of SS Cyg.There are various investigations of SS Cyg, but up to now the cause of its variability, in particular, its rapid variability has not been determined. In addition, there are many other observations which have not been explained.

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Hua, Fei, and Hongqi Wang. "Selective pseudosolubilization capability of Pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis." Frontiers of Environmental Science & Engineering 7, no. 4 (April 2, 2013): 539–51. http://dx.doi.org/10.1007/s11783-013-0498-z.

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Tapia de Daza, M. S., and L. R. Beuchat. "Suitability of modified dichloran glycerol (DG18) agar for enumerating unstressed and stressed xerophilic molds." Food Microbiology 9, no. 4 (December 1992): 319–33. http://dx.doi.org/10.1016/0740-0020(92)80040-b.

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Ismail, Mady A., Hannington K. Taligoola, and Rebecca Nakamya. "Xerophiles and other fungi associated with cereal baby foods locally produced in Uganda." Acta Mycologica 47, no. 1 (December 23, 2013): 75–89. http://dx.doi.org/10.5586/am.2012.009.

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Fifty samples from five baby food products mainly made of cereal flour(s) were analyzed. The moisture contents of these products were between 11.14% and 11.9%, a level below 14.0%, the recommended level for safe storage of cereal grains and their products. The mycological analysis was carried out using the dilution plate method and two isolation media (DG18 for isolation of xerophilic fungi and DRBC for fungi in general). A total of 80 species related to 37 genera in addition to some unidentified fungal and yeast species were recorded on both media from the five products. The products were contaminated abundantly by xerophilic fungi which were occurring in 88% of food samples and accounting for 18.1% of the total CFU as recorded on DG18. The highest contamination level by xerophiles was registered in Mwebaza rice porridge (a component of rice flour) and the lowest in Mukuza (a product of maize, soyabean and sorghum flours). 11 xerophilic species were recorded of which Aspergillus and Eurotium (4 species each) were the predominant giving rise to 9.1% and 8.9% of the total CFU, with A. wentii, A. candidus, E. cristatum and E. repens were the most contaminating species. Of the fungi recorded other than xerophiles, species of Aspergillus (particularly A. flavus followed by A. niger), Penicillium (P. citrinum, P. oxalicum), Fusarium (F. solani, F. tricinctum), Cladosporium (C. sphaerospermum) and yeasts were the most predominant. Contamination of such foods is a matter of health hazard as these foods are for babies. So, the use of fresh, well-dried and uncontaminated flours for production of such foods is recommended.

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Viegas, Carla, Ana Monteiro, Elisabete Carolino, and Susana Viegas. "Occupational exposure to bioburden in Portuguese bakeries: an approach to sampling viable microbial load." Archives of Industrial Hygiene and Toxicology 69, no. 3 (September 1, 2018): 250–57. http://dx.doi.org/10.2478/aiht-2018-69-3116.

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AbstractIn bakeries, a number of operations such as mixing are associated with exposure to air-suspended flour dust and related bioburden. The aim of this study was to find the best active sampling approach to the assessment of occupational exposure to bioburden in Portuguese bakeries based on the data obtained with the use of specific impaction and impinger devices. We used impaction to collect fungal particles from 100 L air samples onto malt extract agar (MEA) supplemented with chloramphenicol (0.05 %). For growing fungi we also used dichloran glycerol (DG18) agar-based media and for mesophilic bacteria we used tryptic soy agar (TSA) supplemented with nystatin (0.2 %). For Enterobacteriaceae we used violet red bile agar (VRBA). With impingers we also collected 300 L air samples at the 300 L/min airflow rate, inoculated onto the same culture media. The two methods, impaction and impinger, showed statistically significant differences in the following counts: fungal on MEA (z=-2.721, p=0.007), fungal on DG18 (z=-4.830, p=0.000), total bacteria (z=-5.435, p=0.000), and Gram-negative coliforms (z=-3.716, p=0.000). In all cases the impaction method detected significantly higher concentrations than the impinger method. Fungal and bacterial loads were higher in the production unit and lower in the shop. The fungal load obtained with impaction varied between 10 and 5140 CFU m-3, and total bacterial counts ranged between 10 and 4120 CFU m-3. This study has shown that the impaction method is the best active sampling approach to assessing viable bioburden in this specific occupational environment, but a multi-faceted approach to sampling and analyses combining methods and media enables a more refined risk characterisation and, consequently, better tailored risk control measures to reduce adverse health outcomes in workers.

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Pfalzgraf, AR, and DP Nau. "DG1: THE RELATIONSHIP OF DIABETES SYMPTOMS AND HEALTH-RELATED QUALITY OF LIFE." Value in Health 4, no. 2 (September 2001): 58. http://dx.doi.org/10.1046/j.1524-4733.2001.40201-29.x.

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Erlbruch, Klaus. "Fortführung der DGK5 beim Ennepe-Ruhr-Kreis mit dem Programm RoSy® Geo Editor–ein Erfahrungsbericht." KN - Journal of Cartography and Geographic Information 51, no. 5 (September 2001): 248–50. http://dx.doi.org/10.1007/bf03544838.

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Kocic-Tanackov, Suncica, Gordana Dimic, Jelena Levic, Ljiljana Mojovic, and Jelena Pejin. "Contamination of cakes with toxigenic molds." Zbornik Matice srpske za prirodne nauke, no. 124 (2013): 213–26. http://dx.doi.org/10.2298/zmspn1324213k.

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The total number of molds in cakes ranged up to 9.0 ? 102 CFU/g. The highest number of molds was isolated on Dichloran 18% Glycerol Agar (DG18), and the lowest on Malt Yeast Extract 50% Glucose Agar (MY50G). Mycopopulation of cakes composed of species of genera Acremonium, Alternaria, Aspergillus, Cladosporium, Eurotium, Mucor, Phialophora, Penicillium, Fusarium and Ulocladium. Dominant species were A. niger, P. aurantiogriseum, P. brevicompactum, P. hirsutum, F. proliferatum and A. alternata. The most common potential producers were those of ochratoxin A (50.50%). The potential producers of fumonisins were present with 7.75%, moniliformin with 6.5% and sterigmatocystin with 0.75%. Potential producers of Alternaria toxins amounted to 4.5%. Aflatoxigenic molds were not isolated from the tested samples. Mycotoxicological analysis of the cakes did not determine the presence of aflatoxin, sterigmatocystin, ochratoxin A and zearalenone.

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Gumilar, Jajang, Lies Mira Yusiati, Ambar Pertiwiningrum, Tomoyuki Nakagawa, and Suharjono Triatmojo. "A novel extracellular keratinase from Exiguobacterium sp. Dg1: enzyme production and dehairing application." Leather and Footwear Journal 17, no. 4 (December 20, 2017): 209–16. http://dx.doi.org/10.24264/lfj.17.4.4.

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Journal articles on the topic 'DG15'

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