Dissertations / Theses on the topic 'Detoxification enzymes'
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Boucher, Ian. "Structural studies of enzymes involved in cell detoxification." Thesis, University of York, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437619.
Full textChalmers, D. "Chemical carcinogenesis : Studies on detoxification enzymes in somatic cell hybrids." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371997.
Full textLabuschagne, Jeanine. "Molecular methods for genotyping selected detoxification and DNA repair enzymes / J. Labuschagne." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4599.
Full textThesis (M.Sc. (Biochemistry))--North-West University, Potchefstroom Campus, 2011.
Cheung, Ka-hong. "Chromate toxicity assessment and detoxification by bacteria from the marine environment /." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36249890.
Full textCheung, Ka-hong, and 張嘉康. "Chromate toxicity assessment and detoxification by bacteria from the marine environment." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45015351.
Full textWhalen, Kristen Elizabeth. "Functional characterization and expression of molluscan detoxification enzymes and transporters involved in dietary allelochemical resistance." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43228.
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Includes bibliographical references.
Understanding how organisms deal with potentially toxic or fitness-reducing allelochemicals is important for understanding patterns of predation and herbivory in the marine environment. The ability of marine consumers to tolerate dietary toxins may involve biochemical resistance mechanisms, which increase the hydrophilicity of compounds and facilitate their active efflux out of sensitive cells and tissues. While several allelochemical-responsive detoxification enzymes have been sequenced and functionally characterized in terrestrial invertebrates feeding on chemically defended host plants, there is virtually no information concerning the role of these biotransformation enzymes that may mediate feeding tolerance in marine invertebrates. The objective of this research was to assess the diversity and dietary regulation of cytochrome P450s (CYP), glutathione S-transferases (GST) and ABC transporters in the generalist marine gastropod Cyphoma gibbosum feeding on a variety of chemically defended gorgonian corals, and to identify those dietary natural products that act as substrates for these proteins. Molecular and proteomic techniques identified both allelochemically-responsive CYPs, and constitutively expressed GSTs and transporters in Cyphoma digestive glands. Inhibition of Cyphoma GST activity by gorgonian extracts and selected allelochemicals (i.e., prostaglandins) indicated that gorgonian diets are likely to contain substrates for molluscan detoxification enzymes. In vitro metabolism studies with recombinant CYPs suggested those Cyphoma enzymes most closely related to vertebrate fatty acid hydroxylating enzymes may contribute to the detoxification ofichthyodeterrent cyclopentenone prostaglandins found in abundance in selected gorgonian species.
(cont.) Finally, the presence and activity of multixenobiotic resistance transporters in Cyphoma and the co-occurring specialist nudibranch, Tritonia hamnerorum, suggests these efflux transporters could function as a first line of defense against dietary intoxication. Together, these results suggest marine consumers that regularly exploit allelochemical-rich prey have evolved both general (GST and ABC transporters) and allelochemical-specific (CYP) detoxification mechanisms to tolerate prey chemical defenses.
by Kristen Elizabeth Whalen.
Ph.D.
Cavka, Adnan. "Biorefining of lignocellulose : Detoxification of inhibitory hydrolysates and potential utilization of residual streams for production of enzymes." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-82486.
Full textBelford, Ebenezer Jeremiah Durosimi. "Purification and characterization of xenobiotic detoxification enzymes in Pachyrhizus "yam bean" and their role in agrochemical metabolism." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=970967012.
Full textWilson, Nina Marie. "Strategies to detoxify the mycotoxin deoxynivalenol and improve food safety in the U.S." Diss., Virginia Tech, 2017. http://hdl.handle.net/10919/77928.
Full textPh. D.
Lee, Sansan. "The effects of knocking down ROS detoxification enzymes on the Caenorhabditis elegans mutants clk-1(qm30) and isp-1(qm150) /." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101597.
Full textDavies, Warren Raymond, and warren davies@optusnet com au. "Effects of the Cyanobacterium Nodularia spumigena on Selected Estuarine Fauna." RMIT University. Applied Sciences, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080415.164533.
Full textDuca, Radu-Corneliu. "Food quality monitoring and analytical techniques optimization of some aliments within plant-animal correlation : Contaminated aliments effects on the detoxification enzymes." Paris 11, 2009. http://www.theses.fr/2009PA11T042.
Full textPoupardin, Rodolphe. "Interactions gènes-environnement chez les moustiques et leur impact sur la résistance aux insecticides." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00583441.
Full textRiaz, Muhammad Asam. "Bases moléculaires de la résistance métabolique au néonicotinoïde imidaclopride chez le moustique Aedes aegypti." Thesis, Grenoble, 2011. http://www.theses.fr/2011GRENV057/document.
Full textMosquitoes transmit several human and animal diseases and their control represents a public health challenge worldwide. In most tropical countries, efficient control of mosquitoes relies on the use of chemical insecticides targeting adults or larvae. However, resistance to the four main classes of chemical insecticides has been reported worldwide and threatens vector control programs. In this context, there is an urgent need to find alternatives to conventional insecticides used in vector control. In this thesis, I explored the potential use of the neonicotinoid insecticide imidacloprid for mosquito control, focusing on the identification of metabolic resistance mechanisms, cross-resistance with other insecticides and the impact of environmental pollutants on imidacloprid tolerance. The mosquito Aedes aegypti was used as a model species for this research work. Basal tolerance of Ae. aegypti to imidacloprid was first evaluated at the larval and adult stages. Effects of a larval exposure across a single generation to a sub-lethal dose of imidacloprid were then investigated at the toxicological and molecular levels using transcriptome profiling. Short sub-lethal exposures were also used to identify potential cross-responses between imidacloprid, other chemical insecticides and anthropogenic pollutants. Long-term adaptive response of Ae. aegypti to imidacloprid was then investigated across several generations by selecting an insecticide-susceptible strain (Bora-Bora strain) with imidacloprid at the larval stage for 14 generations in the laboratory. Such artificial selection allowed obtaining the Imida-R strain. This strain showed an increased resistance to imidacloprid in larvae while no significant resistance was measured in adults. Resistance mechanisms were then investigated using various approaches including the use of detoxification enzyme inhibitors, biochemical assays and transcriptome profiling with DNA microarray and massive mRNA sequencing. Several protein families potentially involved in resistance were identified including detoxifications enzymes and cuticle proteins. Among the formers, 8 cytochrome P450s and 1 glutathione S-transferase appears as good candidates for a role in imidacloprid metabolism. The role of P450s in the elevated resistance of the Imida-R strain was confirmed by comparative P450-dependent in vitro metabolism assays conducted on microsomal fractions of the susceptible and Imida-R strains. At the gene level, substrate binding modeling allowed restricting the panel of P450 candidates. Meantime, heterologous expression of one P450 was performed and its ability to metabolize imidacloprid confirmed. Bioassay with other insecticides revealed potential cross-resistance of the Imida-R at the larval stage to other neonicotinoids but also to an insect growth inhibitor and in a lesser extent to DDT, confirming the probable role of detoxification enzymes. Relaxing the selection pressure of the Imida-R strain for few generations led to a rapid decrease of resistance, suggesting a cost of resistance mechanisms. Comparing the inducibility of candidate detoxification genes by imidacloprid in susceptible and resistant strains revealed a higher induction of these genes in the resistant strain, suggesting the selection of both a higher constitutive expression but also a greater phenotypic plasticity of these enzymes in the Imida-R strain. Finally, the potential role of cuticle protein in resistance was preliminary investigated by exposing larvae to a chitin synthesis inhibitor before bioassays. Overall, although this research work requires additional functional validation experiments, these data provide a better understanding of imidacloprid resistance mechanisms in mosquitoes and its potential use as an alternative to conventional insecticides in vector control
Bourret-Bernard, Claude Sophie. "Effect of lignans in associations with naturally occurring allelochemicals from the Asteraceae on the detoxification enzymes and the life cycle of a herbivorous lepidoptera, Ostrinia nubilalis, Hubner." Thesis, University of Ottawa (Canada), 1988. http://hdl.handle.net/10393/5483.
Full textBorentain, Patrick. "Voies de la glycosylation et carcinome hépatocellulaire." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM5047.
Full textGlycosylation is an enzymatic process that consists of the addition of glycosyl groups to compounds (sugars, lipids or proteins), thus modifying their properties. Glycosylation is involved in the detoxification of xenobiotics and variations in activity of enzymes responsible have been identified as a potential risk factor for cancer in particular in organs in contact with the external environment. In the first part of our work we study the impact of polymorphisms of detoxification enzyme (UGT1A7, GST and XRCC1) on the risk of hepatocellular carcinoma. We show that the combination of genetic polymorphisms of such enzymes may increase the risk of HCC. Modifications in the expression of surface glycoproteins have been observed in cancer cells and play a role in their interactions with the tumoral microenvironment. In the second part, we study the effect of inhibition of interactions of HCC cells / endothelial cells on tumor growth by blocking the interaction between sialyl Lewis x and E-selectin. First, we achieved the inhibition of the expression of sLex on the surface of HCC cells by introducing fucosyl transferase- I gene in HCC cells. In a second part of our work we used cimetidine and amiloride to inhibit the expression of E-selectin by endothelial cells. This approach resulted in inhibition of HCC cells / endothelial cells interaction and thereby tumor growth inhibition in vivo. This effect is mediated by an inhibition of tumor neoangiogenesis. This work therefore identifies genetic risk factors for HCC and allows considering another way of treatment of HCC
Rabêlo, Flávio Henrique Silveira. "Sulfur supply as a sustainable technology for phytoextraction: its effects on cadmium uptake and detoxification in Panicum maximum Jacq. cv. Massai." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-07032019-134024/.
Full textA concentração de cádmio (Cd) no ambiente aumentou em décadas recentes, o que representa sério problema sócio-ambiental, visto que o Cd é tóxico para a maioria dos seres vivos. Por isso, é importante reduzir a concentração desse metal em ambientes contaminados e o uso de plantas adequadamente supridas com enxofre (S) pode contribuir para isso (fitoextração), uma vez que o S é componente de metabólitos envolvidos no combate ao estresse causado pelo Cd. Assim, o nosso objetivo com esse estudo foi avaliar o efeito do S na i) cinética de absorção do Cd, no ii) desenvolvimento radicular e na absorção de nutrientes, no iii) perfil metabólico e síntese de compostos tiol, e iv) na atividade do sistema antioxidante e fotossintético do capim-massai (Panicum maximum Jacq. cv. Massai), utilizado para fitoextração de Cd. Os estudos foram conduzidos em casa de vegetação utilizando-se vasos de 0,5 e 2,0 L para a condução do estudo de cinética de absorção de Cd (tratamentos representados pelas combinações das doses de S de 0,1 e 1,9 mmol L-1 e Cd de 0,1 e 0,5 mmol L-1) e para o estudo dos mecanismos de detoxificação de Cd (tratamentos representados pelas combinações das doses de S de 0,1; 1,9 e 3,7 mmol L-1 e Cd de 0,0; 0,1 e 0,5 mmol L-1), respectivamente. Os vasos foram distribuídos em blocos ao acaso, com quatro repetições. As plantas utilizadas no estudo de cinética foram expostas aos tratamentos durante 14 dias após as mesmas terem permanecido em soluções contendo apenas S durante 42 dias, enquanto as plantas utilizadas no estudo de detoxificação de Cd foram expostas aos tratamentos pelo período de 9 dias após terem crescido em soluções contendo apenas S por 44 dias. Ao final dos estudos, as plantas utilizadas foram colhidas e encaminhadas para análises. A velocidade máxima de absorção (Vmax) e o influxo apoplástico de Cd do capim-massai exposto à maior dose de Cd foram maiores quando as plantas foram supridas com a maior dose de S. O desenvolvimento radicular do capim-massai foi fortemente inibido quando as plantas foram expostas à dose de Cd de 0,5 mmol L-1, mas o adequado (1,9 mmol L-1) suprimento de S permitiu maior absorção de Cd, enquanto o suprimento excessivo (3,7 mmol L-1) de S diminuiu a formação de placas de ferro nas raízes das plantas. A lisina, cisteína, ornitina, arginina, triptofano e histidina foram acumulados em mais de um tecido nas plantas expostas ao Cd, assim como o galactinol. A glutationa (GSH), as fitoquelatinas (PCs) e seus homólogos foram fortemente induzidos pelo Cd, sendo que as plantas supridas com as doses de S de 1,9 e/ou 3,7 mmol L-1 apresentaram as maiores concentrações desses peptídeos. As plantas supridas com as maiores doses de S apresentaram menor peroxidação lipídica e maior taxa fotossintética, o que demonstra que o sistema antioxidante dessas plantas foi mais eficiente na atenuação dos danos causados pelo Cd. Diante desses resultados, fica claro que o capim-massai suprido adequadamente com S apresenta maior tolerância ao Cd, assim como maior potencial de fitoextração
Nascimento, Antonio Rogério Bezerra do. "Bases genéticas e moleculares da resistência de Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) a lufenuron." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11146/tde-04022014-095258/.
Full textThe genetic and molecular basis of resistance to lufenuron in Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) were exploited in this study. The resistant population of S. frugiperda was selected from a population collected in Montevidiu, Goiás. Initially, a luferunon-resistant strain of S. frugiperda was selected from a population collected in cornfields located in Montevidiu, Goiás State, Brazil, with intense use of this insecticide. The diet surface treatment bioassay was used to characterize the concentration-response to lufenuron in the susceptible (SUS) and resistant (LUF-R) strains of S. frugiperda. The estimated LC50s (95% C.I.) for the SUS and LUF-R strains were 0.23 (0.18 - 0.28) and 210.6 (175.90 - 258.10) ?g of lufenuron.mL-1 respectively, with resistance ratio of ? 915-fold. Based on reciprocal crosses between SUS and LUF-R strains, the inheritance of S. frugiperda resistance to lufenuron was incomplete autosomal recessive. Backcrosses between F1 of the reciprocal crosses and the parental LUF-R revealed a polygenic resistance, with an estimation of the minimum number of resistance genes from 1.54 to 1.71, indicating that the number of loci associated to resistance is low. Then, a new high-throughput cDNA sequencing technologies was explored to characterize the transcriptional profile of larvae of Spodoptera frugiperda, and to compare the differential gene expression between resistant and susceptible strains of S. frugiperda to lufenuron in order to identify the resistance mechanism(s) involved. Four cDNA libraries obtained from fourth instars of the resistant (LUF-R) and the susceptible (SUS) S. frugiperda strains, exposed or not to lufenuron, were sequenced in a HiScan1000® platform (Illumina©). The transcriptome was de novo assembled using nearly 19.6 million single-end reads, leading to 18,506 transcripts with a N50 of 996 bp in length. A Blast search against the non-redundant database available in NCBI allowed the functional annotation of 51.1% (9,457) of the obtained transcripts. Most of these transcripts aligned with insect sequences, and a majority of them (45%) with Bombyx mori (Lepidoptera: Bombycidae). Nearly 10% of the transcripts aligned with species belonging to Spodoptera (Lepidoptera: Noctuidae), with 3% of the alignments matching sequences from Spodoptera frugiperda. Differential gene expression analysis between the resistant and the susceptible strains identified 1,224 differentially expressed transcripts (p <= 0.05, t-test; fold change > 2). Seven of them were associated with the cuticle metabolism, and five out seven were up-regulated in the resistant strain (LUF-R). A large set of transcripts (48) associated with the detoxification metabolism was differentially expressed; 40 P450 monooxygenases, five glutathione-Stransferases, two carboxylesterase and one esterase were identified. Thirty-nine out of these 48 transcripts were up-regulated in the resistant strain. Gene expression data obtained by RNA-Seq analysis was validated by quantitative real time PCR (qPCR) of several selected target transcripts. These results represent an important step toward the understanding of the molecular mechanisms of resistance of S. frugiperda to lufenuron, and provide a broader view on the gene expression profile of insects to insecticides.
Marakalala, Mohlopheni Jackson. "Inhibition of a Mycothiol biosynthetic enzyme and a detoxification enzyme as anti-tubercular drug targets." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/2694.
Full textHou, Shurong. "HUMAN BUTYRYLCHOLINESTERASE MUTANTS FOR COCAINE DETOXIFICATION." UKnowledge, 2014. http://uknowledge.uky.edu/pharmacy_etds/38.
Full textKern, Rory James. "Enzyme-based detoxification of organophosphorus neurotoxic pesticides and chemical warfare agents." Thesis, [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-2118.
Full textWagner, Catherine Ann Robertson. "Reproduction and Enzyme Detoxification Activities in Mouse Lines Selected for Response to Fescue Toxicosis." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/9783.
Full textMaster of Science
Liu, Su Qi. "The Activity of Analogs of the Natural Product Dillapiol and Sessamol as Detoxification Enzyme Inhibitors and Insecticide Synergists." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32997.
Full textWorrall, Stephen Frederick. "An investigation into the association between cytochrome P450 and glutathione S-transferase detoxification enzyme polymorphisms and human oral squamous cell carcinoma." Thesis, University of Birmingham, 1998. http://etheses.bham.ac.uk//id/eprint/31/.
Full textGrundling, Daniel Andries. "Cloning and expression of human recombinant isoform a of glycine-N-acyltransferase." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9055.
Full textThesis (MSc (Biochemistry))--North-West University, Potchefstroom Campus, 2013.
Fonseca, Casals Francina. "Pharmacogenomic study of oppioid addicts in methadone treatment / Francina Fonseca Casals." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7234.
Full textThe study recruited opioid dependence patients (DSM-IV criteria) from a MMT community program. Patients were clinically assessed and blood samples were obtained in order to evaluate methadone plasma concentrations of (R,S)-, (R) and (S)- methadone. Allelic variants of genes encoding the following proteins were assessed: BDNF, OPRM1, MYOCD, mGluR6, mGluR8, CRY1, NR4A2, 1q31.2 (rs965972), 2q21.2 (rs1867898), CYP3A5, CYP2D6, CYP2B6, CYP2C9, CYP2C19 and P-glycoprotein. Responders and non-responders were defined by means of illicit opioid consumption detected in random urinalyses.
Differences in response status were found depending on different single nucleotide polymorphisms (SNPs of genes encoding for BDNF, MYOCD and GRM6. The CYP2D6 metabolizing phenotype was associated with response to MMT, and also with methadone dosage requirement and methadone plasma concentrations.
Els programes de manteniment amb metadona (PMM) han demostrat eficàcia en el tractament del trastorn per dependència d'opiacis malgrat la persistència de pacients amb mala resposta al tractament. L'estudi dels factors farmacodinàmics i farmacocinètics implicats en la resposta terapèutica ofereix resultats controvertits. L'objectiu de la tesi doctoral que es presenta és estudiar els factors farmacodinàmics i farmacocinètics de la metadona que poden estar implicats en l'eficàcia del tractament. S'han inclòs pacients ambulatoris diagnosticats de trastorn per dependència d'opiacis (segons criteris DSM-IV) en PMM. Els pacients s'han avaluat a nivell clínic i s'han obtingut mostres de sang per a l'estudi de les concentracions plasmàtiques de (R,S)-, (R) i (S)- metadona. S'han estudiat també les variants al·lèliques dels gens que codifiquen per: BDNF, OPRM1, MYOCD, mGluR6, mGluR8, CRY1, NR4A2, 1q31.2 (rs965972), 2q21.2 (rs1867898), CYP3A5, CYP2D6, CYP2B6, CYP2C9, CYP2C19 i P-glicoproteïna. La mostra s'ha dividit en responedors i no responedors en funció del nombre de controls d'orina positius per a heroïna en analítiques realitzades de forma aleatòria.
Es van detectar diferències en resposta al tractament segons les variants dels gens codificants per a BDNF, MYOCD i GRM6. També es va detectar una associació entre el fenotip de CYP2D6, la resposta al tractament, la dosi requerida de metadona i les concentracions plasmàtiques.
Nardini, Luisa. "Detoxification enzymes associated with insecticide resistance and exposure to entomopathogenic fungi in Anopheles arabiensis." Thesis, 2014.
Find full textAnopheles arabiensis is one of the major African malaria vectors, and DDT and pyrethroid resistance in this species is widespread. The aim of this study was to investigate, in detail, what detoxification enzymes are associated with insecticide resistance using the An. gambiae “detox chip”, a small-scale microarray based on genes that are putatively involved in metabolic detoxification of insecticides. The first part of the study focused on two DDT and pyrethroid resistant laboratory strains of An. arabiensis – one that originated from Sudan, and a second that originated from South Africa. One P450 was over-transcribed in the Sudanese strain, while 20 genes were over-transcribed in the South African strain. The majority of these were P450s although GSTs and redox genes were also over-transcribed. The use of synergist assays indicated that DDT and permethrin resistance were related to the presence of a kdr mutation (determined by PCR), while deltamethrin resistance was based on insecticide metabolism. In order to evaluate the role of enzymatic detoxification in permethrin resistance, a permethrin selected strain was used. No kdr mutations were present in this strain. Here, 29 genes were over-transcribed. Most of these were CYP genes (55%), followed by redox genes (21%), and GSTs (14%). A certain degree of overlap in the gene over-transcription was observed between the deltamethrin and permethrin resistant phenotypes. These genes are potentially functional against both pyrethroids, while those that differed were possibly more substrate specific. The final part of the study aimed to assess whether genes that are associated with insecticide resistance are also induced in mosquitoes infected with the entomopathogen, Beauveria bassiana. Using microarray data, a subset of important insecticide resistance genes was chosen for analysis following fungal infection. This study was based on the use of qPCR to detect changes in expression. None of the genes that were investigated were overtranscribed suggesting that virulence factors, such as toxins, produced by B. bassiana may not be inhibited by genes that are already over-expressed in insecticide resistant mosquito populations. This is promising for biological control and suggests that the fungi are viable alternatives to insecticides.
Chen, Shu-Juan, and 陳淑娟. "Influence of host plants on insecticide tolerance and detoxification enzymes activity of spodoptera litura F." Thesis, 1985. http://ndltd.ncl.edu.tw/handle/91475137235787907383.
Full textChiang, Kuan-Ying, and 姜寬盈. "Correlation Analysis of the Insecticide Resistance and Detoxification Enzymes in the Aedes aegypti (L.) Larvae." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/5z6vk6.
Full text嘉南藥理科技大學
生物科技系曁研究所
97
Field collected Aedes aegypti from each part of Tainan and Kaohsiung districts were subjected to bioassay for their susceptibilities to Temephos, Chlorpyrifos, Fenitrothion, Pirimifosmethyl, Pyrethrins, Permethrin, Fenvalerate, Cypermethrin and compared with two susceptible laboratory strains (NS strain and Bora Bora strain) by using dipping method. The resistance ratio between the 50% lethal concentration value (RR) of the field strains and the NS strain shows that the field strain were widespread, significant resistance to pyrethroid insecticides but not resistance to organophosphorus insecticides. In TPP test (insecticide-insensitive acetylcholinesterase test), the AChE residual activities of field strain compared with susceptible strain were the same in the presence of increasing concentration of propoxur. The TPP test indicate that the point mutation of acetylcholinesterase of Aedes aegypti was not found in the southern of Taiwan. Microplate assays were performed to measure levels of α-esterase, β-esterase, glutathione-S-transferase, monooxygenase enzymes. The patterns of elevate levels of detoxification enzymes in Kaohsiung districts and the Tainan districts strains were different. Comparison between bioassays and biochemical assays, the higher levels glutathione-S-transferase activity was significant correlation with the 50% lethal concentration value of pyrethroid insecticides. The effect of induction detoxification enzymes activity by exposure of Aedes aegypti larvae to sub-lethal dose of permethrin was investigated. The induction detoxification enzymes activity by permethrin in the 97 Tainan East district strain were significant higher than NS strain. The induction effect was no consistency between the experimental field strains in the 1 hr, sub-lethal dose exposure treatment. The mechanisms of insecticide resistance need to be clarified and explored further.
LIU, SHU-XUAN, and 劉淑萱. "The studies of the insecticide susceptibility and the detoxification enzymes of apis cerana and apis mellifera." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/06106814166858789808.
Full textThornton, Benjamin J. "Sex-dependent changes in activity of detoxification enzymes, insecticide susceptibility, and alterations in protein expression induced by atrazine in Drosophila melanogaster." 2009. http://proquest.umi.com/pqdweb?did=1816596021&sid=3&Fmt=2&clientId=14215&RQT=309&VName=PQD.
Full textTitle from title screen (site viewed January 12, 2010). PDF text: v, 131 p. : ill. ; 3 Mb. UMI publication number: AAT 3360086. Includes bibliographical references. Also available in microfilm and microfiche formats.
Belford, Ebenezer Jeremiah Durosimi [Verfasser]. "Purification and characterization of xenobiotic detoxification enzymes in Pachyrhizus "yam bean" and their role in agrochemical metabolism / Ebenezer Jeremiah Durosimi Belford." 2004. http://d-nb.info/970967012/34.
Full textKo, Ya-Ling, and 柯雅羚. "The ethanolic extracts of coriandrum sativum up-regulate the expression of phase II detoxification enzymes and heme oxygenase 1(HO-1)expression in rat clone 9 liver cells." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/03769077197257195766.
Full text中山醫學大學
營養學研究所
101
Coriandrum sativum belongs to the family Apiaceae widely used in Mediterranean, Middle estern and Southeast Asia as spices and herbal medicine. Coriandrum sativum is rich in phenolic compounds wich have been report to lessen inflammation and oxidative stress. Our previous studies demonstrated the anti-inflammatory property of Coriandrum sativum. Here we examined the effect of Coriandrum sativum ethanol extract on expression of phase II detoxification enzymes and heme oxygenase 1 (HO-1) in rat clone 9 liver cells. Coriandrum sativum ethanol extract singnificantly induced HO-1, pi class of glutathione S-transferase (GSTP) and NAD(P)H: quinone oxidoreductase 1 (NQO1) protein and mRNA expression and enzyme activities. Furthermore Coriandrum sativum ethanol extract increased phosphorylation of p38 mitogen-activated protein kinase (p38) and nuclear factor erythroid 2 related factor 2 (Nrf-2) nuclear translocation, Nrf-2 nuclear protein DNA binding activity, as well as antioxidant response receptor (ARE) -luciferase activity and GSTP promoter activity. Pretreatment with SB203580, p38 inhibitor, blocked p38 activation as well as HO-1, GSTP and NQO-1 protein expression induced by Coriandrum sativum ethanol extract. Moreover, silencing of Nrf2 expression by shRNA gene knockdown only inhibiting Coriandrum sativum ethanol extract-induced GSTP and NQO-1 protein expression but not HO-1. These results suggested that Coriandrum sativum up-regulated expression of GSTP and NQO-1 through the p38 and Nrf2/ARE pathway.
Selvi, A. Tamil. "Metallo-β-Lactamase, Phosphotriesterase And Their Functional Mimics." Thesis, 2009. http://hdl.handle.net/2005/994.
Full textGowri, V. S. "Analysis Of Protein Evolution And Its Implications In Remote Homology Detection And Function Recognition." Thesis, 2007. http://hdl.handle.net/2005/568.
Full text張大惠. "Screening, cultivation & enzyme assays of detoxification potential fungi." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/26671949994005901325.
Full textWan-Chi and 廖婉琦. "Effect of piper betle leaf on gene expression of detoxification enzyme in liver." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/2r6eb8.
Full text中山醫學大學
生化暨生物科技研究所
100
Liver is an important organs involved in many physiological roles. Recent research indicates that water extracts of piper betel leaf (PBL) have antioxidant effect. PBL decreased the AST, ALT, and increased SOD, CAT enzyme activities to decrease free radicals induced by CCl4 in rat liver. This effect was elicited via the Ras/Raf signal pathway and induction of TIMP2 to active MMP2 enzyme expression that might enhance the degradation of α-sma, and to ease liver fibrosis. To further understand the hepatoprotective effect of the PBLs, this study use the AAF-induced liver damage model to examine the influence of PBL on the expression of liver detoxification enzymes. Animals were pretreated with PBLs before the induction of AAF, and then PBLs and AAF were given simultaneously. At the end of the study RT-PCR was used to determine the mRNA expression of detoxification enzymes in rat liver that was further correlated to the biochemical markers in sera. The results showed that PBLs alone did not affect liver function. However, AAF influenced the levels of 13 genes in which CYP1A1, GSTM5, and GSTP1 were increased and the rest were decreased. The correlation analysis of the normal group showed that the P450 activity, lymphocyte and platelet were negatively correlated, and became positively correlated in the AAF-induction group. The AAF combined with PBLs group found dose-dependent increases in hepatic nodular lesions and deterioration. However, the combined treatment of PBL did not change the gene expression of AAF, except CYP1A1、CYP2B3、CYP2E1、CYP3A23/3A1. The results show that PBLs did not affect the expression of the detoxification enzymes, indicating that the protective role of PBL may not be through the detoxification metabolic pathway. However, we can not rule out the possibility that the long-term feeding of this study might diminish the immediate impact of betel leaf.
Kaplan, Warren H. "The conformational stability of a detoxification enzyme widely used as a fusion-protein affinity tag." Thesis, 1997. https://hdl.handle.net/10539/26092.
Full textA glutathione S-transferase (Sj26GST) from Schistosoma japonicum, which functions in the parasite's Phase II detoxification pathway, is expressed by the Pharmacia pGEX-2T plasmid and is widely used as a fusion-protein affinity tag. It contains all 217 residues of Sj26GST and an ad titional 9-residue peptide linker with a thrombin cleavage site at its C-terminus. Size-exclusion HPLC (SEC-HPLC) and SDS-PAGE studies indicate that purification of the homodimeric protein under nonreducing conditions results in the reversible for-ration of significant amounts of 160 -kDa and larger aggregates without a loss in catalytic activity. The basis for oxidative aggregation can be ascribed to the high degree of exposure of the four cysteine residues per subunit. The conformational stability of the dimeric protein was studied by urea- and temperature-induced unfolding techniques. Fluorescence-spectroscopy, SEC-HPLC, urea- and temperature-gradient gel electrophoresis, ultraviolet melting, differential scanning micro calorimetry , and enzyme activity were employed to monitor structural and functional changes. The unfolding data indicate the absence of thermodynamically stable intermediates and that the umolding/refolding transition is a two-state process involving folded native dimer and unfolded monomer. The stability of the protein was found to be dependent on its concentration with a ~GO(H20) = 26 ±1.7 kcal/mol. The conformational stability was unchanged in the presence of the leading antischistosomal drug Praziquantel, which bound the protein with a Kd = 9 ±1.8 p,M. The strong relationship observed between the m-v,llue and the size of the protein indicates that the amount of protem. surface exposed to solvent upon unfolding is the major structural de.erminant for the dependence of the protein's free energy of unfolding on urea concentration. 'Ihermograms obtained by differential scanning calorimetry also fitted to a two-state irreversible unfolding transition, both in the presence and absence of Praziquantel, with values of ~Cp = 1779 cal mol-IK-I , ~HcaI = 227 kcal/mol, AHVH ::::::233 kcal/mol (r :::::~:HVHIAlIcal = 1.02) and AS = 354 cal mol''K". The low ~Cp and ~S, when compared with the theoretically determined values, implied that the thermal denaturation of Sj26GST did not result in complete unfolding of the protein,
Andrew Chakane 2018
Van, der Sluis Rencia. "Investigation and characterisation of the genetic variation in the coding region of the glycine N-acyltransferase gene / Rencia van der Sluis." Thesis, 2015. http://hdl.handle.net/10394/15639.
Full textPhD (Biochemistry), North-West University, Potchefstroom Campus, 2015