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1

Wurdani, Eresha Melati Kusuma, Adioro Soetojo, and Devi Eka Juniarti. "Differences in tensile adhesion strength between HEMA and nonHEMA-based dentin bonding applied on superficial and deep dentin surfaces." Dental Journal (Majalah Kedokteran Gigi) 50, no. 1 (March 31, 2017): 14. http://dx.doi.org/10.20473/j.djmkg.v50.i1.p14-18.

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Background: Improvement in dentistry shows some progresses, due to patients awareness on the importance of dental care. Cervical lesion is the most common phenomenon which oftenly found 46.36% in man and 38.13% in woman. Cervical lesions need composite restoration for treatment to stop the process of tissue damage. The process of adhesion of composite restoration material to the structure of the tooth is not easily separated and it needs optimal function in the oral cavity. Application of dentin bonding agents to attach the composite is needed. Selection of HEMA-based bonding material and Hema free-based bonding material which have a different solvent in their composition, as applied to the dentin superficial and deep dentin, affect the results of debonding test. Debonding test is done to measure the adhesion strength of a bonding material. Purpose: The purpose of this study was to analyze differences in tensile bond strength of dentine bonding HEMA-based and HEMA-free based after application in superficial and deep dentine surfaces. Method: The tooth of the bovine was as samples. A superficial dentine sample was taken from 0.5-1 mm of dentino enamel junction and a deep dentine sample was taken from 0.5 mm culmination of pulp horn. Dentine surface area was equal to p x r2 = (3.14 x 22) = 12.56 mm2. Six samples of HEMA-based bonding was applied to the dentine superficial. Six samples of HEMAfree based bonding was applied to the superficial dentine. Six samples of HEMA-based bonding was applied to the deep dentine. Six samples of HEMA-free based bonding was applied to the deep dentine. Tensile strength was measured using an Autograph AG-10TE. Result: There were differences tensile bond strength of dentine bonding HEMA-based and HEMA-free based after the application on superficial (p=0.000) and deep dentine surfaces (p=0.000). Conclusion: There were differences tensile bond strength of dentine bonding HEMA-based and HEMA-free based after the application on superficial and deep dentine surfaces. The use of dentine bonding materials HEMA-free based were better than HEMA-based after application on different dentine depths.
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2

Румянцев, В. А., А. Р. Бессуднова, А. В. Блинова, and Н. В. Заблоцкая. "ЭФФЕКТИВНОСТЬ ИМПРЕГНАЦИИ ДЕНТИНА ЗУБОВ НАНОЧАСТИЦАМИ ГИДРОКСИДА МЕДИ-КАЛЬЦИЯ ПРИ ЭКСПЕРИМЕНТАЛЬНОМ ЛЕЧЕНИИ КАРИЕСА." MEDICINE, SCIENCE AND EDUCATION, no. 34 (November 2022): 51–55. http://dx.doi.org/10.56936/18291775-2022.34-51.

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For the prevention of recurrent and secondary caries of teeth, as well as bullets, antiseptic treatment of the carious cavity, dentine adhesives, laser radiation, etc. are used. However, these methods do not allow achieving effective decontamination and obturation of dentine tubules, in which pathogenic microflora can continue to persist. In the experimental treatment of dentin caries using electron microscopy, the effectiveness of a new technique of galvanophoretic nanoimpregnation of dentin with nanoparticles of a complex preparation of copper-calcium hydroxide was studied. Galvanophoresies was performed using an aluminum foil in contact with a copper-containing preparation. After instrumental treatment of the cavity, it is advisable to carry out nanoimpregnation of dentin for 14 days. During this period, 85-90% of the dentine tubules are obturated to an average depth of 800 microns and the dentine is saturated with calcium, which contributes to the restoration of its mineral composition, disturbed as a result of microbial demineralization. The effectiveness of nanoimpregnation of dentin with copper-calcium hydroxide decreases with age of the teeth, as well as with existing carious cavity.
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3

Rasni, Novia D. P., and Johanna A. Khoman. "Penatalaksanaan Hipersensitivitas Dentin." e-GiGi 9, no. 2 (May 4, 2021): 133. http://dx.doi.org/10.35790/eg.9.2.2021.33885.

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Abstract: Dentin hypersensitivity is one of the most common dental problems. It can be found in both sexes, especially in the elderly. The discomfort or pain experienced in cases of dentin hypersensitivity occurs due to the unprotected surface by enamel in the crown or by cementum in the tooth root area. The characteristic of dentin hypersensitivity is that the pain suffered is acute, sharp but short in the unprotected dentin. This review was aimed to discuss about the etiology, stimulatory transmission mechanisms, and management of dentine hypersensitivity.Keywords: dentin hypersensitivity; pain Abstrak: Hipersensitivitas dentin merupakan salah satu masalah gigi yang paling sering dijumpai, dapat ditemui pada laki-laki maupun perempuan utamanya pada orang yang sudah lanjut usia. Ketidaknyamanan atau rasa ngilu yang dialami pada kasus hipersensitivitas dentin terjadi karena adanya permukaan yang tidak terlindungi oleh email di mahkota atau sementum di daerah akar gigi. Ciri khas hipersensitif dentin yaitu rasa sakit yang diderita bersifat akut, tajam tapi singkat pada dentin yang tidak terlindungi. Penulisan ini bertujuan untuk membahas mengenai etiologi, mekanisme penjalaran rangsang dan tata kelola kasus hipersensitivitas dentin.Kata kunci: hipersensitivitas dentin; rasa ngilu
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4

Kinney, J. H., S. Habelitz, S. J. Marshall, and G. W. Marshall. "The Importance of Intrafibrillar Mineralization of Collagen on the Mechanical Properties of Dentin." Journal of Dental Research 82, no. 12 (December 2003): 957–61. http://dx.doi.org/10.1177/154405910308201204.

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It is widely held that the hardness and modulus of dentin increase in proportion to the mineral concentration. To test this belief, we measured hardness and modulus of normal dentin and an altered form of dentin without gap-zone mineralization in wet and dry conditions by AFM nanoindentation to determine if the modulus and hardness scale linearly with mineral concentration. Mineral concentrations in the mid-coronal location of the normal and altered dentins were 44.4 vol% and 30.9 vol%, respectively. Surrounding the pulp of the altered dentin was a region of higher mineralization, 40.5 vol%. The indentation modulus of normal dentin was 23.9 (SD = 1.1) GPa dry and 20.0 (SD = 1.0) GPa wet. In mid-coronal regions of the altered dentin, the indentation modulus was 13.8 (SD = 2.0) GPa dry and 5.7 (SD = 1.4) GPa wet. In the more mineralized regions of the altered dentin, the modulus was 20.4 (SD = 1.8) GPa dry and 5.3 (SD = 0.8) GPa wet; the properties of the altered wet dentin did not correlate with mineral concentration. The results of this study raise doubt as to whether mineral concentration alone is a sufficient endpoint for assessing the success or failure of remineralization approaches in restorative dentistry.
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Berahim, Zurairah, Keyvan Moharamzadeh, Adrian K. Jowett, and Andrew Rawlinson. "Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-DimensionalIn VitroModel of Periodontium." International Journal of Dentistry 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/605813.

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The aim of this study was to develop a three-dimensionalin vitromodel of periodontium to investigate the osteogenic and cementogenic differentiation potential of the periodontal ligament fibroblast (PDLF) spheroids within a dentin-membrane complex. PDLFs were cultured in both spheroid forms and monolayers and were seeded onto two biological collagen-based and synthetic membranes. Cell-membrane composites were then transferred onto dentin slices with fibroblasts facing the dentin surface and further cultured for 20 days. The composites were then processed for histology and immunohistochemical analyses for osteocalcin, Runx2, periostin, and cementum attachment protein (CAP). Both membranes seeded with PDLF-derived cells adhered to dentin and fibroblasts were present at the dentin interface and spread within both membranes. All membrane-cell-dentine composites showed positive staining for osteocalcin, Runx2, and periostin. However, CAP was not expressed by any of the tissue composites. It can be concluded that PDLFs exhibited some osteogenic potential when cultured in a 3D matrix in the presence of dentin as shown by the expression of osteocalcin. However the interaction of cells and dentin in this study was unable to stimulate cementum formation. The type of membrane did not have a significant effect upon differentiation, but fibroblast seeded-PGA membrane demonstrated better attachment to dentin than the collagen membrane.
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6

Kumar R., Sarath, Deepti Jawa, Rani Somani, Shipra Jaidka, Oinam Renuka, Mayanglambam Leleesh, Hridya V.G, and Serene MS. "REVIEW ON BIODENTINE - A BIODENTINE DENTINE SUBSTITUTE." International Journal of Advanced Research 10, no. 02 (February 28, 2022): 606–15. http://dx.doi.org/10.21474/ijar01/14245.

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Biodentine is a tricalcium silicate-based cement designed for permanent dentin replacement material and has drawn attention in recent years. Biodentine has been adviced to be used in various clinical applications such as pulp caping, apexification,root perforations, retrograde fillings and dentine replacement. There has been considerable research performed on this material since its launching however, there is scarce number of review articles that collates information and data obtained from these studies. Therefore, this review article was prepared for providing a general picture regarding the characteristics of the material. Biodentine is a good biocompatible and bioactive material. It helps in the protection of underlying pulp by inducing tertiary dentin formation. Unlike other dentin substitutes, Biodentine application does not require any conditioning of the dentin surface. The restoration sealing of Biodentine is micromechanical retention by penetrating into the dentin tubules forming tag-like structures. After its setting, Biodentine can be reshaped like natural dentin. Published clinical trials, histology of human teeth and clinical cases show that Biodentine has a wide spectrum of clinical applications as a permanent bulk dentin substitute in pediatric dentistry and restorative dentistry as a possible replacement material.
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7

Johnson, Lynn, Sonal GP Pillai, and Palak Upadhyay. "Investigation of Dentinal Tubule Occlusion by a Desensitizing Dentifrice: an in-Vitro Sem Analysis." Journal of Nursing Research,Patient Safety and Practise, no. 34 (June 29, 2023): 11–16. http://dx.doi.org/10.55529/jnrpsp.34.11.16.

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The purpose of this study was to compare patent dentin tubules to in vitro dentin tubules in order to assess the dentin tubule occluding impact of a dentifrice incorporating Pro-Argin Technology (Colgate Sensitive Plus). Methods: The diameter of the dentine tubule was measured using scanning electron microscopy (SEM) on 20 produced dentin discs that were either left untreated or treated with the dentifrice. To evaluate tubule patency, microphotographs were obtained at a 2000x magnification. The dentifrice covered the treated sample, filling the dentinal tubule and decreasing or closing the tubule opening, according to SEM imaging results. Conclusion: According to pertinent statistical analysis, Pro-argin technology efficiently blocked dentinal tubules, making it suitable for use as a dental anti-sensitivity agent.
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DEMARCO, Flávio Fernando, Miriam Lacalle TURBINO, and Edmir MATSON. "COHESIVE STRENGTH OF DENTIN RESISTÊNCIA COESIVA DA DENTINA." Revista de Odontologia da Universidade de São Paulo 11, no. 3 (July 1997): 189–94. http://dx.doi.org/10.1590/s0103-06631997000300007.

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The bond strength of dentin adhesives to dentin has increased after each generation. Although dentin substratum is part of the bonding process, little importance has been given to measure dentin cohesive strength. The aim of this study was to evaluate the cohesive strength of dentin in human canines. Seventeen non carious canines were selected. All of them had been extracted for more than one year. The teeth were ground until dentin square samples with approximately 2 X 2 mm were obtained. They were embedded in acrylic resin and subjected to shear stress, in a Wolpert Machine, at a crosshead speed of 0.5 mm/min. The mean cohesive strength of dentin in shear mode was 33.95 (+-9.72) MPa. The fracture surfaces were observed under a X40 magnification. A finite element analysis was performed to observe the stress distribution as related to the shear test. The failure pattern was compatible with the shear test and also with the stress distribution in the finite element analysis
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9

Sher, Bryan Martin, Riaan Mulder, and Norbert Gutknecht. "The Photobiomodulation Effect of 940nm Laser Irradiation on Enterococcus faecalis in Human Root Dentin Slices of Varying Thicknesses." Journal of Lasers in Medical Sciences 12, no. 1 (August 30, 2021): e48-e48. http://dx.doi.org/10.34172/jlms.2021.48.

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Introduction: An increase in dentine thickness could result in an inadequate depth of laser energy penetration. This study aimed to evaluate the effect of a 940 nm laser on Enterococcus faecalis through varying thicknesses of human root dentin slices. Methods: Thirty-five dentin slices of root dentin with thicknesses ranging between 500 and 3000 µm were produced. Six experimental groups (500, 1000, 1500, 2000, 2500, and 3000 µm (n=5 each) were lased and the seventh, non-lased group served as the positive control with a dentine thickness of 2000 µm. The slices were inoculated with 2 µL of E. faecalis suspension of 1.5 × 108 E. faecalis cells/mL. All the lased slices were lased from the opposing side of the inoculation. A non-initiated 200 μm bare end fibre at the power of 1 W, in a continuous wave was used. Four doses of laser irradiation of 5 seconds with a side to side movement with the tip held at a 5º angle to the dentine slice were performed. The colony-forming units of E. faecalis were determined and the bacterial photobiomodulation effect analysed using one-way ANOVA with a Bonferroni and Holm post hoc test at a significance level of P>0.05. Results: There were statistical differences between the dentin slices of 500, 1000, and 1500 μm treated with the laser compared to the positive control (P<0.01). However, there were no statistical differences between the lased 2000 and 2500 μm slices compared to the positive control. There was significantly more photobiomodulation of the E. faecalis for the dentine slices of 3000 μm than the positive control (P<0.01). Conclusion: Laser treatment through dentine slices of 2000 μm and thinner significantly reduced bacterial growth. The photobiomodulation effects started to occur in dentine slices thicker than 2500 μm compared to the positive control.
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10

Oancea, Roxana, Cristian Funieru, Ruxandra Sfeatcu, Delia Berceanu Vaduva, and Adelina Cheveresan. "Evaluation of the Impact Induced by High Dietary Sucrose Concentration and Glucocorticoid Medication on Dentin Formation of Young Rats." Revista de Chimie 70, no. 10 (November 15, 2019): 3714–18. http://dx.doi.org/10.37358/rc.19.10.7631.

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The impact of sucrose and systemic cortisone treatment on the response of dentin was examined in an experimental rat model. After 2 months of a modified diet and/or medication period, the areas of dentin formation and dentinal caries were quantified in the mandibular molars of growing animals. The 60% sucrose diet significantly reduced dentin formation and increased dentinal caries progression. The presence of glucocorticoid medication alone reduced dentin formation. Cortisone medication in combination with sucrose diet further decreased dentin apposition. Rats receiving the control diet positively responded to the dentinal caries by increasing dentin formation to prevent pulpal exposure. The rate of dentine formation was lower in rats with high sucrose diet and predentin area was wider compared to the control group fed normal diet. The enlargement of the predentin region in sucrose diet rats reflects changes in the functionality of the odontoblasts, such as reduction of matrix synthesis and alteration of the mineralization process. These results indicate that the functional alterations in the pulpo-dentinal complex might contribute to dentinal caries progression through systemic mechanism irrespective of the causative factors.
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Bhargava, Karan, Srinidhi Surya Raghavendra, Sanjyot Mulay, Ajit Hindlekar, Avinash Kharat, and Supriya Kheur. "Evaluating the ability of cultivated odontoblasts to form dentin-like tissue in vitro using fibroblast growth factor and insulin-like growth factor." Journal of Conservative Dentistry and Endodontics 27, no. 6 (June 2024): 598–602. http://dx.doi.org/10.4103/jcde.jcde_174_24.

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Abstract Aim: The aim of the study was to evaluate the ability of cultivated odontoblast to form dentin-like tissue using fibroblast growth factor (FGF) and insulin-like growth factor (IGF). Materials and Methods: Dental pulp stem cells (DPSCs) were extracted from 10 human teeth. They were isolated and cultivated in vitro with the use of stem cell markers. The human DPSCs were characterized for trilineage differentiation. They were then differentiated into odontoblasts. The ability of cultivated odontoblasts to form dentin-like tissue was evaluated using FGF and IGF. Results: IGF showed superior ability to form dentin-like tissue as compared to FGF. The addition of FGF showed no significant difference in the formation of dentin-like tissue. A combination of FGF and IGF in odontoblast showed an enhanced ability to form dentin-like tissue. Conclusion: The use of growth factors IGF and FGF with dental stem cells showed a greater potential to form dentin-like tissue. This can profoundly alter the paradigms of conservative vital pulp therapy, which may eventually make it possible to treat dental diseases by regeneration of lost dentine.
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Mohan, Sumit, Jyoti Thakur, Santosh Kumar Verma, Rima Jaiswal, Abhishek Verma, and Shresth Kumar Bhagat. "Prevalence of dentin hypersensitivity in Bihar and Jharkhand- A multicentric study." UNIVERSITY JOURNAL OF DENTAL SCIENCES 6, no. 3 (January 12, 2021): 68–73. http://dx.doi.org/10.21276/ujds.2020.6.3.13.

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Aim: Dentine hypersensitivity is a common oral problem. This pilot study investigated the prevalence of dentine hypersensitivity in the diverse population across four centres of Bihar and Jharkhand. Materials and Methods: A multi-stage, random sampling method was used to investigate the study population. A total of 5622 subjects were examined at four centres in Bihar and Jharkhand. Subjects were divided into 8 age groups. Participants completed a dentine hypersensitivity questionnaire and underwent clinical examination. The diagnosis of dentine hypersensitivity was confirmed. Gingival recession of sensitive teeth was measured by a Williams periodontal probe. Results: Among 5622 subjects, 1253 were found to be suffering from dentin hypersensitivity indicating a prevalence of 22.28%. Females were more prone to dentin hypersensitivity with male to female ratio of 1:1.31. While subjects in age group of 50-59 were predominantly affected, the molars and premolars were commonly affected with dentin hypersensitivity. Conclusion: The prevalence of dentine hypersensitivity in East India was 22.28%, indicating that it is a common condition. For its effective management, public education about the condition and effective treatment of dentine hypersensitivity are required. Clinical Significance: Assessment of prevalence of hypersensitivity in this demographical location would enable the dental care health workers to devise strategies to educate masses about the prevention and management of this disease
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Stojsin, Ivana, Ljubomir Petrovic, Igor Stojanac, and Milan Drobac. "Multi-factoriality of dentine hypersensitivity." Medical review 61, no. 7-8 (2008): 359–63. http://dx.doi.org/10.2298/mpns0808359s.

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Introduction. Dentine hypersensitivity has been defined as a sharp, short pain arising from exposed dentin in response to stimuli typically thermal, evaporative tactile, osmoticor, chemical and which cannot be ascribed to any other form of dental defect or pathology. Prevalence. The most affected patients range in age from 20 to 40. The following teeth tend to be most sensitive: cuspids, premolars and incisors, location-concentrated on the facial surface. Morphological bases of dentine hypersensitivity Sensitive teeth have much greater numbers of open tubules per unit area and the average diameter of tubules is almost 2 times greater than tubules in nonsensitive teeth. Mechanisms of dentine hypersensitivity. The most widely accepted theory of how the pain occurs is Brannstroms theory. Etiology Dentine hypersensitivity represents a condition of presumable multifactorial pathology. Two processes are essential for its development: (1) dentin must be exposed through either genetic disturbance, enamel defect (lamellae, tufts and spindles), loss of enamel (erosion, abrasion, attrition, abfraction), gingival recession with rapid loss of cementum and (2) the dentin tubules must be open to both the oral cavity and the pulp. Diagnosis. Diagnostic protocol for this condition consisted of Medical, Dental Dietary, Oral Hygiene History and Intra-oral examinations with air indexing method. Differential Diagnosis: We must take into consideration a number of variables such as: dental caries, cracked tooth, restorative sensitivity, medication sensitivity, bleaching sensitivity and abscessed or non-vital tooth. Conclusion. Dentin hypersensitivity is a problem that bothes many patients. Many conditions share the symptoms of tooth sensitivity so differential diagnosis is essential for suitable treatment or preventive measures.
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Filho, E. J. A., P. R. P. Motoyama, J. S. Mendonça, J. C. Silva, and T. A. D. Mendes. "A Eficácia Clínica da Técnica Úmida Etanólica em Restaurações de Resina Composta: Revisão de Literatura." Journal of Health Sciences 19, no. 5 (February 23, 2018): 95. http://dx.doi.org/10.17921/2447-8938.2017v19n5p95.

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O objetivo do presente estudo foi revisar a literatura a cerca da eficácia clínica na técnica úmida etanólica (TUE) em restaurações com resinas compostas. Foi realizada uma busca nas bases de dados PubMed, Bireme e Cochrane Library de artigos em inglês do período de 2007 a 2017, utilizando os seguintes descritores: Dentin, Dentin-Bonding Agents e Ethanol como estratégia de busca. Foram encontrados 101 artigos, dos quais apenas os ensaios clínicos foram selecionados, totalizando 6, mediante a relevância, sendo os demais artigos excluídos do presente estudo. A TUE consiste na substituição de água livre em dentina por solvente livre de água, etanol absoluto. A técnica simplificada consiste na aplicação de etanol absoluto por 1 minuto, previamente a aplicação do “Bond”, de adesivo convencional de 3 passos. A TUE tem se mostrado bastante promissora, pois vai haver a remoção de água presente na dentina, através de desidratação química e um encolhimento das fibras colágenas permitindo que os monômeros hidrofóbicos penetrem mais facilmente em dentina, sem o uso de “primers”. Dessa forma, diminuirá a sorção dos monômeros, consequentemente a hidrólise e a degradação da interface de união restauração/adesivo, aumentando a longevidade da restauração. Entretanto quando se trata de estudos clínicos a sua eficácia ainda não é bem estabelecida, acredita-se que seja por ação da pressão pulpar. Dessa forma, são necessários mais estudos clínicos e um maior período de observação para avaliar a durabilidade clínicas de restaurações com essa técnica.Palavras-chave: Dentin. Dentin-Bonding. Agents. Ethanol.
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Dewina Marsha Larasati and Shafira Kurnia Supandi. "Management of gingival recession with modified coronally advanced tunnel technique: Case report." World Journal of Advanced Research and Reviews 22, no. 3 (June 30, 2024): 1488–92. http://dx.doi.org/10.30574/wjarr.2024.22.3.1820.

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Introduction: Gingival recession is characterized by apical migration of the gingival margin from the cementoenamel junction (CEJ), with concomitant exposure of the root surface. Teeth with gingival recession often experience pain in response to thermal, chemical, and tactile stimuli to the exposed dentine. This is known as dentin hypersensitivity. Dentin hypersensitivity is characterized by exposed dentinal tubules most often due to gingival recession and loss of cementum through erosion, abrasion, or other factors. Patient’s main concerns: A-20-years-old male patient visited to Periodontics Clinic Dental Hospital Airlangga University with the main complaint of dentin hypersensitivity in the upper right second premolar. The Systemic is good, had no history of allergies and did not smoke. Important clinical findings: The recession measured 1 mm facially on the maxillary right second premolar. Case and Management: Prior to surgery, scaling and root planing was performed to eliminate debris and calculus. Two weeks afterward, modified coronally advanced tunnel technique was done for dentin hypersensitivity treatment. One month evaluation, dentin hypersensitivity disappeared. Conclusion: Modified coronally advanced tunnel technique was the appropriate treatment to treat dentin hypersensitivity. This technique achieved healthy keratinized tissue and coverage of the root surface.
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DIMITRIU, BOGDAN, ECATERINA IONESCU, IOANA SUCIU, DRAGOS EPISTATU, DANA BODNAR, MIHAELA CHIRILA, STEFAN MILICESCU, et al. "Tertiary dentin deposition in the proximity of the cervical perforation aperture – A case study." Romanian Biotechnological Letters 26, no. 2 (February 2, 2021): 2523–26. http://dx.doi.org/10.25083/rbl/26.2/2523.2526.

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Dead tracts are dentin areas characterized by degenerated odontoblastic processes; may result from injury caused by caries, attrition, erosion, or cavity preparation. Odontoblasts can also form tertiary dentin, as a response to injury, in association with caries, trauma, or restorative procedures. Generally, this dentin is less organized than primary and secondary dentin and mostly localized to the site of injury. The reactive dentin formation that lays under caries, the pulp displays chronic inflammation and tertiary dentinogenesis takes place on the inner walls of the pulp space, in the region of the dentinal tubules associated with the base of the carious lesion. Higher-power photomicrograph of tertiary dentin shown in a primary dentin, first period of tertiary dentin formation, second period of tertiary dentin formation. We initiated a case study regarding depositition of tertiary dentin in the close proximity of the cervical perforation hole on mesial root of the second maxillary molar. The repair material used (Biodentine, Septodont) was found placed under the tertiary dentin layer, rather than at the perforation place, mentioning the fact that the group of residents was not experienced with the management of the endodontic microscope and did not use a proper field. We consider that we have identified dead tracts due to the fact that they were formed consequently carious lesions pathology, thereafter the tertiary dentin at the appearance of the coronal aspect was identified at the end of the dead tracts. The portion of the canal entrances were investigated both with analyzed filters (Crossed Polars) and with compensatory devices (retardation plates). The present study identified tertiary dentine deposits in the coronary portion of the adjacent root canal entered in the perforation. At a thorough analysis of the coronal portions of the mesial root canal with compensating devices and analyzers filters, it was noticed a soft dentin blanket in which the dentinary tubules do not exist or are of very rare deposition.
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Stojsin, Ivana, Tatjana Brkanic, and Slavoljub Zivkovic. "The effect of gastric juice on the development of erosive changes in hard dental tissue." Srpski arhiv za celokupno lekarstvo 142, no. 7-8 (2014): 413–18. http://dx.doi.org/10.2298/sarh1408413s.

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Introduction. Gastroesophageal reflux disease (GERD) is an esophageal disorder where the refluxed gastric contents enters first into the esophagus followed by the pharynx, oral cavity, larynx, airway and middle ear, causing a range of disorders and symptoms. Hydrochloric acid from the gastric contents is responsible for the demineralization of dental hard tissues and release of matrix metalloproteinase from the dentin. Objective. The aim of this study was to verify the SEM (scanning electron microscopy) analysis of the surface enamel, the enamel-dentin border and dentine after the exposure of intact teeth to filtrate of gastric contents obtained during routine endoscopy. Methods. Material used in the research was 10 extracted human impacted third molars. The coronal part of the tooth was divided into two parts, and then the two halves of teeth were exposed to the filtrate of gastric juice obtained during routine gastroscopy, which had been frozen until the moment of the experiment initiation. All samples of teeth were immersed in the filtrate of the content at a temperature of 20?C for 60 minutes. The prepared samples were observed by the SEM in the area of the enamel, the enamel-dentin border and in the area of dentin at different magnification. Results. The SEM analysis showed that both enamel and dentin had a significant demineralization of these tissues. Enamel surface resembled a demineralization similar to that of acid conditioning before the application of composite restorations. The degree of mineralization was more intense towards the enamel - dentin border, and at this area the enamel prisms were not fully recognizable. The dentin had a complete loss of peritubular dentin, the entry points of the dentin tubules were expanded and intertubular dentin demineralization was also registered. Conclusion. SEM analysis showed a significant degree of destruction of enamel and dentin. Significant changes in the surface structure of enamel and dentin were also registered. The filtrate of the gastric juice degrades enamel pellicle and the dentin organic component.
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Shalamay, L. I., E. Yu Mendosa, E. E. Maiorov, V. B. Lampusova, and N. S. Oksas. "Obtaining data on the penetration of the chemical curing adhesive into the dentin when using different techniques of bond applying." Stomatology for All / International Dental review, no. 4(105) (December 25, 2023): 24–29. http://dx.doi.org/10.35556/idr-2023-4(105)24-29.

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A study aimed at obtaining tomographic data on the penetration of the chemical curing adhesive into the dentin when using different bond application techniques was carried out. High-quality aesthetics, durability, functionality and maximum preservation of hard dental tissues are the result of new adhesive preparation and improvement of the physico-chemical and optical properties of composites, therefore, the study of the optical properties of composites is relevant and promising. The paper presents the appearance of the spectrophotometer, an optical scheme is given and the technical characteristics of the device are given. Samples for studies made in various techniques are presented, and the measurement channel of the device is also shown, where samples were installed to measure the boundaries of the interaction of dental adhesive and dentin in depth. The spectral dependences of the absorption coefficient in the zone of the fixation boundaries of composite tabs with dentin and the boundary areas of the samples are obtained when using the adhesive according to the protocol described in the instructions; when applying with an additional 10-second exposure on the dentine before inflating; when rubbing of the adhesive into the dentine surface for 10 seconds before inflating. The data of the bond penetration into the dentin with usual techniques was revealed.
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19

Simões, Tânia Christina, Maynara Gongora Rubim, Rodrigo Varella de Carvalho, André Tomazini Gomes de Sá, Wagner Alberto Madallozo Torres, and Sandra Kiss Moura. "Effect of Polyacrylic Acid Conditioning on the Longevity of Bonding of Self - Adhesive Resin Cement to Dentin." Journal of Health Sciences 20, no. 2 (July 27, 2018): 100. http://dx.doi.org/10.17921/2447-8938.2018v20n2p100-105.

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AbstractBonding of fiber posts using self-adhesive resin cement to radicular dentin is controversial. The aim was to evaluate the effect of conditioning of polyacrylic acid on the longevity of bonding of self adhesive resin cement to dentin. Thirty six single rooted pre molars were divided into 3 groups (n=12) ARC: ScotchBond Multi Purpose + RelyX™ARC; U: RelyX™U200; PU: 11.5% Polyacrylic acid + RelyX™U200. After conditioning of the dentin, the resin cement and the post were inserted and light-activated for 60s (1200mw/cm2) and stored in distilled water (24h/37ºC). Two discs of 1 mm thickness were obtained from each root-third and tested in push-out (0.5mm/min) after 24 hours and 6 months. For statistical purposes, the data from the root thirds were grouped and the bond strength (in MPa) was analyzed using Two-Way Analysis of Variance and Tukey’s post-hoc test at 5%. The power of the test was calculated. The bonding of the U at 6 months was higher than PU at both times (p<0.001). There were no differences for time (p=0.153) or for the interaction of treatment x time (p=0.090). Adhesive fractures between the cement and dentin were prevalent. The power test was 0.97. The bonding of the RelyX™U200 was influenced by the dentin pre-treatment with poliacrylic acid and the bonding of all groups remained stable for 6 months.Keywords: Dental Cements. Dentin. Shear Strength.ResumoA adesão de pino de fibra de vidro com cimento resinoso autoadesivo à dentina é controversa. Foi objetivo avaliar o efeito do condicionamento da dentina com ácido poliacrílico na longevidade da adesão de cimento resinoso autoadesivo. Trinta e seis raízes de pré-molares uni radiculados foram divididas em 3 grupos (n=12) ARC: ScotchBond Multi Purpose + RelyX™ARC; U: RelyX™U200; PU: Ácido poliacrílico 11,5% + RelyX™U200. Após condicionamento da dentina, o cimento resinoso e o pino de fibra de vidro foram inseridos, fotoativados por 60s (1200mw/cm2) e armazenados em água destilada (24h/37ºC). Dois discos de dentina de 1 mm de espessura foram obtidos de cada terço radicular e testados em push-out (0.5mm/min) após 24 houras e 6 meses. Para análise estatística, os dados dos terços radiculares foram agrupados e resistência adesiva (em MPa) analisada por Análise de Variância de dois fatores e teste de Tukey a 5%. O poder do teste foi calculado. A adesão de the U após 6 meses foi maior que a de PU nos dois períodos (p<0,001). Não houve diferença para o fator tempo (p=0,153) nem para a interação tratamento x tempo (p=0,090). Fraturas adesivas prevaleceram entre cimento e dentina. O poder do teste calculado foi de 0,97. A adesão do RelyX™U200 foi influenciada pelo pré-tratamento da dentina com ácido poliacrílico e a adesão de todos os grupos se manteve estável até 6 meses.Palavras-chave: Cimentos Dentários. Dentina. Resistência ao Cisalhamento.
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20

Mujahid, Afsa, Samina Kanwal, Zehra Ahmed, Anum Nisar, Syeda Noor Israr, and Raffat Aziz. "Dentine Hypersensitivity: A cross-sectional study in Karachi." Pakistan Journal of Medical and Health Sciences 17, no. 5 (May 27, 2023): 68–71. http://dx.doi.org/10.53350/pjmhs202317568.

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Aim: To assess the awareness of dentinal hypersensitivity among the patients visiting dental outpatient department at KMDC hospital, Karachi. Methodology: The data for this cross-sectional study was collected from 361 patients visiting dental OPD of Karachi Medical and Dental College, Karachi. After informed consent, a close ended type questionnaire was filled and patients were examined for dentin hypersensitivity. The data obtained was analysed using SPSS version 22. Result: Out of total participants, 40% male and 21.5% female significantly respond to dentin hypersensitivity (p-value 0.001). Dentin hypersensitivity was higher in males than in females .40-50 age group was more commonly affected and cold stimuli was the most common aggravating and initiating factor found. In addition to it, Aggressive tooth brushing and grinding are the main etiological factors for dentin hypersensitivity. Practical implication: This study may help to clearly understand the prevalence and associated risk factors of dentine hypersensitivity to develop effective and appropriate treatment. Conclusion: It is necessary to organize public awareness programmes regarding adequate brushing technique, good oral hygiene, avoidance of abrasive as well as erosive agents and counselling the dietary habits to prevent dentin hypersensitivity. Keywords: Personal awareness, dentin hypersensitivity, hydrodynamic theory, public awareness, aetiology, erosion
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21

Alves Teixeira, Raquel, Marcelo Henrick Matochek Maia, Pedro Luiz Santos Tomaz, Thales De Sá Oliveira, Mackeler Ramos Polassi, Fabiano Vieira Vilhena, and Paulo Henrique D'Alpino. "Caracterização Físico-Química e Efetividade de Dentifrícios Dessensibilizantes na Redução da Permeabilidade Dentinária." Ensaios e Ciência C Biológicas Agrárias e da Saúde 25, no. 5-esp. (March 14, 2022): 729–34. http://dx.doi.org/10.17921/1415-6938.2021v25n5-esp.p729-734.

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O uso de dentifrícios dessensibilizantes é uma opção prática e eficaz para minimizar a hipersensibilidade dentinária por obliterarem túbulos dentinários expostos. Objetivou-se avaliar in vitro a efetividade de dentifrícios dessensibilizantes na redução da permeabilidade dentinária após escovação e desafio erosivo. Trinta e seis discos de dentina bovina (1 mm espessura) foram escovados com escova elétrica (Oral-B Vitality Precision Clean rechargeable toothbrush) por dois minutos com os seguintes dentifrícios (n=9): Crest Colgate Pró-Alívio Imediato, Sensodyne Repair & Protect e Regenerador Diário DentalClean. A permeabilidade dentinária foi avaliada em 4 tempos experimentais: inicial (controle, não tratado), após a primeira escovação, após escovação diária por sete dias e após desafio ácido (ácido cítrico; 0,05M) por cinco minutos. Calculou-se a permeabilidade dentinária por condutividade hidráulica (em μl/min.cm H²O.cm²). Os dados foram analisados estatisticamente (ANOVA 2 critérios/Tukey (5%). A permeabilidade dentinária reduziu, significativamente, após a escovação inicial (p<0,05), à exceção do Regenerador Diário DentalClean, que apresentou permeabilidade similar ao controle. Após 7 dias, houve uma redução significativa na permeabilidade para todos os dentifrícios em relação ao controle (p<0.05). Porém, após o desafio erosivo, houve um aumento significativo da permeabilidade dentinária para o dentifrício Crest. Conclui-se a permeabilidade dentinária variou em função do tratamento, sendo todos dentifrícios efetivos após 7 dias; porém somente Colgate Pró-Alívio Imediato, Sensodyne Repair & Protect e Regenerador Diário DentalClean foram efetivos em manter reduzida a permeabilidade após desafio erosivo. Palavras-chave: Sensibilidade da Dentina. Erosão Dentária. Dentifrícios. Dentina. Abstract The use of desensitizing toothpastes is a practical and effective option to minimize dentinal hypersensitivity by obliterating exposed dentinal tubules. The objective was to evaluate in vitro the desensitizing gels effectiveness in reducing dentin permeability after brushing and erosion challenge. Thirty-six bovine dentin discs (1 mm thick) were brushed with an electric brush (2 minutes) with the following toothpastes (n = 9): Crest, Colgate Pro-Relief Immediate, Sensodyne Repair & Protect and DentalClean Daily Regenerator. Dentin permeability was evaluated in 4 experimental times: initial (control, untreated), after the first brushing, after daily brushing for 7 days and after acid challenge (citric acid; 0.05M) for 5 min. Dentin permeability was calculated by hydraulic conductivity (in μl / min.cm H²O.cm²). The data were analyzed statistically (ANOVA 2 criteria / Tukey (5%). The dentin permeability significantly decreased after the initial brushing (p <0.05), with the exception of Daily Regenerator which showed similar permeability to the control. After seven days, there was a significant reduction in the permeability for all gels compared to the control (p <0.05). However, after the erosive challenge, there was a significant increase in the dentin permeability for the toothpaste Crest, concluding the dentin permeability varied depending on the treatment, all of which were effective after 7 days, but only Colgate Pro-Relief, Sensodyne Repair & Protect, and Regenerador Diário DentalClean were effective in keeping the permeability reduced after an erosion challenge. Keywords: Dentin Sensitivity. Tooth Erosion. Dentifrices. Dentin.
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Alves Teixeira, Raquel, Marcelo Henrick Matochek Maia, Pedro Luiz Santos Tomaz, Thales De Sá Oliveira, Mackeler Ramos Polassi, Fabiano Vieira Vilhena, and Paulo Henrique D'Alpino. "Caracterização Físico-Química e Efetividade de Dentifrícios Dessensibilizantes na Redução da Permeabilidade Dentinária." Ensaios e Ciência C Biológicas Agrárias e da Saúde 25, no. 5-esp (March 14, 2022): 729–34. http://dx.doi.org/10.17921/1415-6938.2021v25n5-espp729-734.

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O uso de dentifrícios dessensibilizantes é uma opção prática e eficaz para minimizar a hipersensibilidade dentinária por obliterarem túbulos dentinários expostos. Objetivou-se avaliar in vitro a efetividade de dentifrícios dessensibilizantes na redução da permeabilidade dentinária após escovação e desafio erosivo. Trinta e seis discos de dentina bovina (1 mm espessura) foram escovados com escova elétrica (Oral-B Vitality Precision Clean rechargeable toothbrush) por dois minutos com os seguintes dentifrícios (n=9): Crest Colgate Pró-Alívio Imediato, Sensodyne Repair & Protect e Regenerador Diário DentalClean. A permeabilidade dentinária foi avaliada em 4 tempos experimentais: inicial (controle, não tratado), após a primeira escovação, após escovação diária por sete dias e após desafio ácido (ácido cítrico; 0,05M) por cinco minutos. Calculou-se a permeabilidade dentinária por condutividade hidráulica (em μl/min.cm H²O.cm²). Os dados foram analisados estatisticamente (ANOVA 2 critérios/Tukey (5%). A permeabilidade dentinária reduziu, significativamente, após a escovação inicial (p<0,05), à exceção do Regenerador Diário DentalClean, que apresentou permeabilidade similar ao controle. Após 7 dias, houve uma redução significativa na permeabilidade para todos os dentifrícios em relação ao controle (p<0.05). Porém, após o desafio erosivo, houve um aumento significativo da permeabilidade dentinária para o dentifrício Crest. Conclui-se a permeabilidade dentinária variou em função do tratamento, sendo todos dentifrícios efetivos após 7 dias; porém somente Colgate Pró-Alívio Imediato, Sensodyne Repair & Protect e Regenerador Diário DentalClean foram efetivos em manter reduzida a permeabilidade após desafio erosivo. Palavras-chave: Sensibilidade da Dentina. Erosão Dentária. Dentifrícios. Dentina. Abstract The use of desensitizing toothpastes is a practical and effective option to minimize dentinal hypersensitivity by obliterating exposed dentinal tubules. The objective was to evaluate in vitro the desensitizing gels effectiveness in reducing dentin permeability after brushing and erosion challenge. Thirty-six bovine dentin discs (1 mm thick) were brushed with an electric brush (2 minutes) with the following toothpastes (n = 9): Crest, Colgate Pro-Relief Immediate, Sensodyne Repair & Protect and DentalClean Daily Regenerator. Dentin permeability was evaluated in 4 experimental times: initial (control, untreated), after the first brushing, after daily brushing for 7 days and after acid challenge (citric acid; 0.05M) for 5 min. Dentin permeability was calculated by hydraulic conductivity (in μl / min.cm H²O.cm²). The data were analyzed statistically (ANOVA 2 criteria / Tukey (5%). The dentin permeability significantly decreased after the initial brushing (p <0.05), with the exception of Daily Regenerator which showed similar permeability to the control. After seven days, there was a significant reduction in the permeability for all gels compared to the control (p <0.05). However, after the erosive challenge, there was a significant increase in the dentin permeability for the toothpaste Crest, concluding the dentin permeability varied depending on the treatment, all of which were effective after 7 days, but only Colgate Pro-Relief, Sensodyne Repair & Protect, and Regenerador Diário DentalClean were effective in keeping the permeability reduced after an erosion challenge. Keywords: Dentin Sensitivity. Tooth Erosion. Dentifrices. Dentin.
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23

Lafuente, David, and Isabel Ferreto. "Immediate Dentin Bond Strength of Self-etch Dentine Adhesives." Journal of Research in Dentistry 2, no. 1 (February 28, 2014): 51. http://dx.doi.org/10.19177/jrd.v2e1201451-57.

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Purpose: The purpose of this study was to evaluate the immediate bond strength of two sixth generation and two seventh generation dentin bonding agents to superficial dentin. Specimens were prepared to exposed superficial dentin and either Clearfil SE, Clearfil S3, Adper Prompt-l-pop of G-bond was applied over the dentin surface and light cured. Then composite resin was applied to the treated surface and light-cured in two increments. Specimens were tested 15 minutes after they were made for shear bond strength at 0.01 cm/min. Clearfil SE had statistically higher bond strength than the other three adhesives evaluated (42.9 MPa). There was no statistical difference among Clearfil S3, Adper Prompt-l-pop and G-Bond. The dentin adhesive with an application of an acidic primer before the application of the adhesive showed better immediate bond strength.
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24

Baruwa, Abayomi Omokeji, Jorge N. R. Martins, Tatjana Maravic, Claudia Mazzitelli, Annalisa Mazzoni, and António Ginjeira. "Effect of Endodontic Irrigating Solutions on Radicular Dentine Structure and Matrix Metalloproteinases—A Comprehensive Review." Dentistry Journal 10, no. 12 (November 23, 2022): 219. http://dx.doi.org/10.3390/dj10120219.

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Irrigating solutions play an important role in the eradication of intracanal microbes and debris dissolution during endodontic treatment. Different combinations of solutions and protocols have been advocated, with sodium hypochlorite (NaOCl), ethylenediamine tetra acetic acid (EDTA), and chlorhexidine (CHX) remaining the most widely used ones by many clinicians. Although these solutions provide efficient inorganic dissolution and antimicrobial capacity, their use has also been reported to cause undesired effects on root dentin composition and mechanical and biomechanical properties, such as microhardness, surface roughness, bond strength, and matrix metalloproteinase (MMP) activity. Several corroborating studies attribute these changes in mechanical properties of dentine to the use of irrigating solutions, and there are limited reports on how the solutions affect the expression of MMPs, which may be a correlating link to understanding the role of these enzymes in dentin collagen and changes in the mechanical properties of dentin. Hence, using the basis of several studies from the literature, the objective is to comprehensively review the influence of individual and combined irrigating solutions on root dentine structure and the activity of the MMPs.
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25

Akhmedbeyli, R. M. "Enamel and dentin microhardness of deciduous and permanent teeth formed in fluoride-iodine deficiency." Kazan medical journal 99, no. 4 (August 8, 2018): 625–28. http://dx.doi.org/10.17816/kmj2018-625.

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Aim. To study the microhardness of enamel and dentin of deciduous and permanent teeth formed in fluoride-iodine deficiency. Methods. Enamel and dentin microhardness was examined on sections of 47 deciduous and 43 permanent teeth. Six topographic zones were examined on each tooth section, 24 imprints were performed. Enamel and dentin microhardness was determined on PMT-3M device and by Vicker’s Hardness Test with a load of 50 g for 5 seconds. Results. Enamel and dentin microhardness of permanent teeth is higher than that of deciduous ones. In the surface layer of enamel the microhardness of permanent teeth is 2.33-2.09 times higher compared to deciduous teeth (in incisors - by 2.24 times, canines - by 2.09 times, molars - by 2.33 times); 2.25-1.94 times higher in the middle layer (in incisors - by 2.17 times, canines - by 1.94 times, molars - by 2.25 times); 2.15-1.89 times higher in the enamel layer at the enamel-dentine junction (in incisors - by 2.15 times, canines - by 1.89 times, molars - by 2.03 times). In the dentin layer at the enamel-dentin junction, dentin microhardness of permanent teeth is 1.90-1.71 times higher than of deciduous ones (in incisors - by 1.78 times, canines - by 1.71 times, molars - by 1.90 times); 2.14-2.0 times higher in the middle dentin layer (in incisors - by 2.08 times, canines - by 2.0 times, in molars - by 2.14 times); 2.05-1.71 times higher in the near-pulp layer (in incisors - by 2.05 times, canines - by 1.71 times, molars - by 1.87 times). Conclusion. Enamel and dentin microhardness of deciduous and permanent teeth formed in fluoride-iodine deficiency varies considerably; microhardness of hard tissues of permanent teeth is approximately 2 times higher than that of deciduous teeth.
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26

Абдулина, Ю., Y. Abdulina, С. Григорьев, S. Griroriev, П. Панфилов, and P. Panfilov. "Estimates of the strength of the connection «enamel — plomb» and «dentin — plomb» in permanent teeth in middle age after etching gel containing Ag." Actual problems in dentistry 12, no. 1 (March 25, 2016): 4–10. http://dx.doi.org/10.18481/2077-7566-2016-12-1-4-10.

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The summary The study of features of strength and microstructure of joints «enamel-filling» and «dentin-sealing» after the etching treatment agent containing Ag. Samples of tissues were fixed with adhesive composite material for mechanical testing in uniaxial compression. The study shows that the content of the etching agent, silver does not affect the bond strength of dentin with composite material. Metallographic investigation of the microstructure of the joints and the bordering regions of dentin showed the status of the border and its ability to suppress the growth of cracks. In the work by means of optical and scanning electron microscopes, microstructure of studied compounds affected dentine secondary caries in permanent teeth close to the border «dentin-sealing» after the etching treatment agent containing Ag. The presence in the etching agent nanocolloidal silver does not affect the adhesive strength of the boundary of the hard tissues of the tooth with a composite material and does not change the color of the hard tissues.
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27

Lima, Joyce Figueiredo Macedo de, Danielle Wajngarten, Fariya Islam, Johanna Clifford, and Ana Carolina Botta. "Effect of adhesive mode and chlorhexidine on microtensile strength of universal bonding agent to sound and caries-affected dentins." European Journal of Dentistry 12, no. 04 (October 2018): 553–58. http://dx.doi.org/10.4103/ejd.ejd_239_18.

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ABSTRACT Objective: The main objective of this study is to assess the effect of the adhesive mode and chlorhexidine (CHX) on the microtensile strength of a universal bonding agent to sound and caries-affected dentins. Materials and Methods: Six intact third molars and six decayed third molars were sectioned to obtain two middle dentin specimens per tooth. Prime and Bond Elect Universal (Dentsply) was applied to specimens in two different modes: Etch-and-rinse (ER, phosphoric acid for 15s) and self-etch (SE, no phosphoric acid). 2% CHX (Cavity Cleanser, Bisco) was applied to both dentins for 30 s. Specimens were randomly assigned into eight groups (n = 3) according to the treatments applied to sound and caries-affected dentins: SE; ER; SE + CHX; and ER + CHX. All specimens were restored with TPH Spectra High Viscosity (Dentsply) composite resin and sectioned to obtain specimens of 0.8 ± 0.1 mm2. The microtensile bond strength test was conducted at 1 mm/min. The data were statistically analyzed by ANOVA and multiple comparisons' test (α = 0.05). Results: There was no significant difference between sound dentin and caries-affected dentin (P = 0.132). The highest bond strength was obtained with the application of the ER (31.81MPa). SE + CHX promoted the lowest bond strength with no statistical difference to ER + CHX (P > 0.05). Conclusions: ER mode for the universal bonding agent yielded the highest bond strength to sound and caries-affected dentins. CHX reduced the bond strength to both dentins regardless the application of phosphoric acid. Clinical Significance: The universal bonding agent increased the bond strength to sound and caries-affected dentins when applied by the ER mode.
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28

Gangishetti, Sairam, Amulya Kolluri, K. Amit Raj, Deepika Kamsani, Saikrishna Manchala, and Deepa Jarupula. "Bioactivity of Calcium Silicate-Based Endodontic Materials: A Comparative in vitro Evaluation." Journal of Pharmacy and Bioallied Sciences 16, Suppl 2 (April 2024): S1716—S1720. http://dx.doi.org/10.4103/jpbs.jpbs_959_23.

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ABSTRACT Background: Bioactivity refers to the ability of a material to interact with living organisms or biological systems in a way that elicits a specific response. In the context of materials science and medicine, bioactivity is particularly important because it can determine the suitability of material for various applications. Objective: To evaluate and compare different commercially available calcium silicate-based materials regarding: 1. Morphological and elemental analysis at the dentin/material interface. 2. Calcium and silicon release and uptake by adjacent root canal dentine by evaluating the calcium and silicon incorporation depth in adjacent root canal dentin. Materials and Methods: This study examined four calcium silicate-based cements: Biodentine, MTA Angelus, BioAggregate, and MTA Plus. One hundred extracted human teeth with intact apices and no cavities were selected. Root sections measuring 3 mm in length were created at the mid-root level using low-speed diamond discs. Bioactivity was evaluated at 1, 7, 30, and 90 days, respectively. Results: The principal composition of the interfacial dentine layer and incorporation of calcium and silicon into dentine was measured at 1, 7, 30, and 90 days. Statistical analysis was performed by multiple comparisons using post hoc Tukey HSD. Conclusion: All the materials have shown bioactivity, i.e. release of calcium, silicon, and their uptake in the adjacent dentin in the presence of phosphate-buffered saline.
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Astudillo-Ortiz, Esteban, Pedro S. Babo, Rui L. Reis, and Manuela E. Gomes. "Evaluation of Injectable Hyaluronic Acid-Based Hydrogels for Endodontic Tissue Regeneration." Materials 14, no. 23 (November 30, 2021): 7325. http://dx.doi.org/10.3390/ma14237325.

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Dental pulp tissue engineering (TE) endeavors to regenerate dentin/pulp complex by combining a suitable supporting matrix, stem cells, and biochemical stimuli. Such procedures foresee a matrix that can be easily introduced into the root canal system (RCS) and tightly adhere to dentin walls to assure the dentin surface’s proper colonization with progenitor cells capable of restoring the dentin/pulp complex. Herein was investigated an injectable self-setting hyaluronic acid-based (HA) hydrogel system, formed by aldehyde-modified (a-HA) with hydrazide-modified (ADH), enriched with platelet lysate (PL), for endodontic regeneration. The hydrogels’ working (wT) and setting (sT) times, the adhesion to the dentine walls, the hydrogel’s microstructure, and the delivery of human dental pulp cells (DPCs) were studied in vitro. Hydrogels incorporating PL showed a suitable wT and sT and a porous microstructure. The tensile tests showed that the breaking point occurs after 4.3106 ± 1.8677 mm deformation, while in the indentation test after 1.4056 ± 0.3065 mm deformation. Both breaking points occur in the hydrogel extension. The HA/PL hydrogels exhibited supportive properties and promoted cell migration toward dentin surfaces in vitro. Overall, these results support using PL-laden HA injectable hydrogels (HA/PL) as a biomaterial for DPCs encapsulation, thereby displaying great clinical potential towards endodontic regenerative therapies.
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30

Luppieri, Valentina, Antonio Manfra, Luca Ronfani, Maddalena Chermetz, and Milena Cadenaro. "Ozone Therapy for Early Childhood Caries (ECC) Treatment: An In Vivo Prospective Study." Applied Sciences 12, no. 4 (February 14, 2022): 1964. http://dx.doi.org/10.3390/app12041964.

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The present study aimed to evaluate the effectiveness of ozone therapy in (1) increasing the compactness of decayed dentin (main aim), (2) reducing dentin hypersensitivity, (3) decreasing the salivary bacterial count, and (4) improving the quality of life in ECC-affected children during a 3-month follow-up. A total of 20 subjects uncooperative with conventional restorative treatment were treated with a 60 s ozone application/week (OzoneDTA®) for 4 weeks. Patients were evaluated at T0 = baseline; T1 = after ozone cycle and T2, T3, T4 = 1, 2, 3-month follow-up, respectively. Dentin compactness was evaluated according to the Affected Dentine Scale (ADS) proposed by the authors; dentin hypersensitivity was recorded with the Wong–Baker Faces Pain Rating Scale (WBFPRS); salivary bacteria were measured with the Saliva Check Mutans Test and quality of life with the Early Childhood Oral Health Impact Scale (ECOHIS). ADS values and salivary bacteria count results were analyzed using non-parametric tests. Statistically significant differences were detected for both parameters between T0 and T1 (p < 0.01). At T1, dentin hypersensitivity was resolved and quality of life improved. Ozone therapy is a simple and painless approach, which might be useful for controlling caries’ progression in uncooperative children until appropriate cooperation is achieved.
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Shellis, R. Peter, Michele E. Barbour, David M. Parker, Martin Addy, and Adrian Lussi. "Effects of Calcium and Phosphate on Dissolution of Enamel, Dentin and Hydroxyapatite in Citric Acid." SWISS DENTAL JOURNAL SSO – Science and Clinical Topics 133, no. 7/8 (July 10, 2023): 432–38. http://dx.doi.org/10.61872/sdj-2023-07-08-02.

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The aim was to evaluate the effect of dissolved calcium and phosphate on dissolution rate of enamel, dentin and compressed hydroxyapatite (HA) in citric acid solution as a function of pH. At pH 2.5, dissolution rate of enamel increased significantly by 6% in 20 mmol/L added calcium but, otherwise, dissolution rates of neither enamel, dentin nor HA were significantly affected by 10 or 20 mmol/L calcium. However, enamel dissolution rate was reduced by > 50 mmol/L calcium. At pH 3.25 and 4.0, 10-20 mmol/L calcium inhibited dissolution of enamel by 29-100% and HA by 65-75% but did not affect dentin dissolution. Phosphate (10 or 20 mmol/L) did not inhibit dissolution of enamel, dentin or HA at any pH, but there were increases in dissolution rate of all three substrates at pH 2.5 and, in one test with dentine (at 20 mmol/L phosphate), at pH 3.25. The results suggest that calcium addition to soft drinks and other acidic products such as medications may reduce erosivity against enamel, provided that pH is not too low; that phosphate would not reduce erosivity against enamel; and that neither calcium nor phosphate at these concentrations would reduce erosivity against dentin.
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32

Merza, Apriko, Billy Sujatmiko, and Rinda Yulianti. "Perbandingan kekerasan mikro dentin mahkota setelah aplikasi berbagai bahan bleaching intrakoronal." Majalah Kedokteran Gigi Indonesia 2, no. 3 (December 30, 2016): 128. http://dx.doi.org/10.22146/majkedgiind.11261.

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Comparing microhardness of dentine crown after application of various intracoronal bleaching agents. The aim of this study is to compare microhardness of dentine crown after treatment with intracoronal bleaching agents. The method of this study was an experimental laboratory. Thirty two extracted human mandibular first premolars without caries, sectioned at 2 mm below Cemento-Enamel Junction were divided into four groups and bleaching agents were sealed into the pulp chambers as follows: group A – 45% carbamide peroxide, group B – 35% hydrogen peroxide, group C – sodium perborate mixed aquadest and group D – aquadest. Access cavities were sealed and then stored in aquadest at 37 °C. Bleaching procedures were performed on days 0, 7, 14 and 21. After 28 days, the teeth were sectioned longitudinally, and planted on acrylyc. Microhardness of dentine crown was measured by vickers microhardness tester. One Way ANOVA and LSD were used to evaluate the effect of intracoronal bleaching agents on microhardness of dentine crown. The results showed that average values of microhardness of dentine crown on group A was 45,04 VHN, group B was 45,42 VHN, group C was 55,22 VHN and group D was 55,63 VHN. In clonclusion, there was si gnificantly different microhardness of dentine crown between group 45% carbamide peroxide and 35% hidrogen peroxide with sodium perborate mixed aquadest, but between group 45% carbamide peroxide with 35% hidrogen peroxide there was no significant difference.ABSTRAKTujuan penelitian ini untuk mengetahui perbandingan kekerasan mikro dentin mahkota setelah aplikasi berbagai bahan bleaching intrakoronal. Jenis penelitian ini merupakan penelitian eksperimental laboratoris. Sebanyak 32 gigi premolar pertama mandibula tanpa karies, telah diekstraksi, dipotong 2 mm di bawah cemento-enamel junction dibagi dalam 4 kelompok dan bahan bleaching dimasukkan ke dalam kamar pulpa, yaitu kelompok A – 45% karbamid peroksida, kelompok B -35% hidrogen peroksida, kelompok C - sodium perborat dikombinasikan dengan aquadest, dan kelompok D – aquadest. Akses kavitas ditutup kemudian disimpan di dalam aquadest dengan suhu 37 °C. Prosedur bleaching dilakukan pada hari ke-0, 7, 14 dan 21. Setelah 28 hari, mahkota gigi dipotong secara longitudinal dan salah satu bagian ditanam di akrilik. Nilai kekerasan mikro dentin mahkota diuji menggunakan Vickers microhardnes tester. One way ANOVA dan uji LSD digunakan untuk mengevaluasi pengaruh berbagai bahan bleaching intrakoronal terhadap kekerasan mikro dentin. Hasil penelitian menunjukkan nilai kekerasan mikro dentin mahkota pada kelompok A sebesar 45,04 VHN, kelompok B sebesar 45,42 VHN, kelompok C sebesar 55,22 VHN dan kelompok D sebesar 55,63 VHN. Kesimpulan dari penelitian ini terdapat perbedaan kekerasan mikro dentin mahkota yang signifikan antara kelompok 45% karbamid peroksida dan 35% hidrogen peroksida dengan sodium perborat dikombinasikan dengan aquadest, sedangkan antara kelompok 45% karbamid peroksida dengan 35% hidrogen peroksida tidak terdapat perbedaan yang signifikan.
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Sa’ada, Maiada Mohammad Ahmad, Nagwa Mohammed Ali Khattab, and Maha Ishaq Amer. "Effect of Silver Diamine Fluoride Pretreatment on Shear Bond Strength of Resin Modified Glass Ionomer Cement to Primary Dentin." Open Access Macedonian Journal of Medical Sciences 9, no. D (September 20, 2021): 243–47. http://dx.doi.org/10.3889/oamjms.2021.7093.

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AIM: The aim of the study was to evaluate effect of silver diamine fluoride (SDF) on the shear bond strength (SBS) of glass ionomer restoration to primary dentine. MATERIALS AND METHODS: Twenty extracted primary molars with no restorations or developmental anomalies were only chosen for the study. Twenty dentine slices with 2 mm thickness were prepared and then randomly allocated into two groups (n = 10 per group). For Group 1 (experimental): Dentine surfaces were treated with 38% *SDF solution (Elevate oral care, USA) for 3 min using micro-brush, followed by a 30 s rinse with water and Group 2 (Control): Dentine surfaces were treated with distilled water for 3 min then rinsed for 30 s. The cavity conditioner was applied to flat dentin of both groups for 10 s then washed and dried gently with compressed air. All the specimens were restored with *RMGIC (Riva Light Cure, SDI, Australia). SBS was determined using a Universal Testing machine and Failure mode was evaluated using stereomicroscope. Data were collected and statistically analyzed. Independent t test was used to assess differences in SBS between study groups and mode of failure was analyzed using Fisher’s exact test. Level of significance was set at p ≤ 0.05. IBM SPSS statistical software (version 26) was used to analyze the data. RESULTS: The experimental group had significantly higher mean of shear bond (11.92 ± 3.35) strength than the control group (6.99 ± 2.98) (p = 0.003). CONCLUSIONS: Primary dentin pre-treatment with 38% SDF increases the shear bond strength between RMGIC and primary dentin according to the results of this in vitro study.
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Ozan, Günçe, Meriç Berkman, and Hande Șar Sancaklı. "Dentin degradomics in dentin erosion." STOMATOLOGY EDU JOURNAL 9, no. 1 (2022): 55–62. http://dx.doi.org/10.25241/stomaeduj.2022.9(1).art.6.

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Background Dentin degradomics are the enzymes found in dentin endogenously and are aimed at attacking organic compounds of the relevant tissue. During dentin demineralization, these enzymes could turn into the reaction phase and may step up the degradation. Thus, their connection with dentin erosion and tissue loss should be explained. Objective The aim of this review was to describe the mechanisms of dentin degradomics, their relation to dentin erosion, and recent approaches on inhibiting their action. Data sources A narrative review was performed with a literature search in the PubMed and Google Scholar electronic databases. Study selection Reference lists included full papers of any study design, published in peer-reviewed journals in English till November 2021. Data extraction Current literature indicates the term of dentin degradomics, and the mechanism of dental erosion of both enamel and dentin tissues. The inhibition of matrixmetalloproteinase (MMP) enzymes, which constitute the subgroup of dentin degradomics, was gained from the recent papers listed in the reference section. Data synthesis Biocorrosion covers more of the pathological process of the tissue loss however, most of the dentin degradomics such as MMPs are not covered by the term, biocorrosion. So, the definitions of biocorrosion and dentin degradomics were discussed in detail. Green tea, chlorhexidine and fluorides have the ability to inhibit the reaction of MMPs during dentin demineralization with a different state of mechanisms. Nowadays, other naturally-derived compounds were included in studies such as polyphenols and flavonoids. Still, more studies are necessary to clarify their mechanism of action and rates of efficiency.
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Yamakoshi, Yasuo. "Dentin Sialophosphoprotein (DSPP) and Dentin." Journal of Oral Biosciences 50, no. 1 (January 2008): 33–44. http://dx.doi.org/10.1016/s1349-0079(08)80016-3.

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Ozan, Günçe, Meriç Berkman, and Hande Șar Sancaklı. "Dentin degradomics in dentin erosion." STOMATOLOGY EDU JOURNAL 9, no. 1-2 (2022): 55–62. http://dx.doi.org/10.25241/stomaeduj.2022.9(1-2).art.6.

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Background: Dentin degradomics are the enzymes found in dentin endogenously and are aimed at attacking organic compounds of the relevant tissue. During dentin demineralization, these enzymes could turn into the reaction phase and may step up the degradation. Thus, their connection with dentin erosion and tissue loss should be explained. Objective: The aim of this review was to describe the mechanisms of dentin degradomics, their relation to dentin erosion, and recent approaches on inhibiting their action. Data sources: A narrative review was performed with a literature search in the PubMed and Google Scholar electronic databases. Study selection: Reference lists included full papers of any study design, published in peer-reviewed journals in English till November 2021. Data extraction: Current literature indicates the term of dentin degradomics, and the mechanism of dental erosion of both enamel and dentin tissues. The inhibition of matrixmetalloproteinase (MMP) enzymes, which constitute the subgroup of dentin degradomics, was gained from the recent papers listed in the reference section. Data synthesis: Biocorrosion covers more of the pathological process of the tissue loss however, most of the dentin degradomics such as MMPs are not covered by the term, biocorrosion. So, the definitions of biocorrosion and dentin degradomics were discussed in detail. Green tea, chlorhexidine and fluorides have the ability to inhibit the reaction of MMPs during dentin demineralization with a different state of mechanisms. Nowadays, other naturally-derived compounds were included in studies such as polyphenols and flavonoids. Still, more
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Grinberga, Sanita, Evaggelia Papia, Jolanta Aleksejuniene, Vita Zalite, Janis Locs, and Una Soboleva. "Effect of Temporary Cement, Surface Pretreatment and Tooth Area on the Bond Strength of Adhesively Cemented Ceramic Overlays—An In Vitro Study." Dentistry Journal 11, no. 1 (January 5, 2023): 19. http://dx.doi.org/10.3390/dj11010019.

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Several viewpoints have been reported regarding the effect of temporary cements, different surface pretreatment protocols before adhesive cementation, and predictive factors. This in vitro study tested if temporary cement, pretreatment of the tooth surface, the size of enamel or dentine influence adhesive cementation to zirconia ceramics. Twenty premolars were prepared for determination of enamel and dentin area, bond strength test and failure analysis. The samples were divided into two groups: untreated prior adhesive cementation (n = 10) and with temporary cementation done, pretreated prior adhesive cementation (n = 10). Zirconia overlays (Katana Zirconia STML) were cemented on the grounded flat teeth surfaces using Panavia V5. An additional six premolars underwent dentine tubule analysis with SEM to detect temporary cement residues after temporary cementation on an untreated tooth surface (n = 3) and on a pretreated surface (n = 3). The independent sample t-test was used to compare the two groups and the means of the total tooth, dentin or enamel areas did not differ significantly between the untreated and pretreated specimens. The mean tensile bond strength was significantly (p = 0.005) higher in the pretreated specimens (337N) than in the untreated ones (204N). The overall multivariable linear regression model with three predictors (surface pre-treatment, enamel area and dentine area) was significant (p = 0.003), among which the size of enamel was the strongest predictor (β = 0.506; p = 0.049), followed by the pretreatment effect (β = 0.478; p = 0.001) and the size of dentin area (β = −0.105; p = 0.022).
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Singh, Himanshu, Vedant Patel, Rahul A. Razdan, Deepak Jha, Prachi Nayak, and Sushruth Nayak. "Secondary Dentin as an Aid in Gender Predilection: A Forensic Study." Dental Journal of Advance Studies 09, no. 03 (November 10, 2021): 147–50. http://dx.doi.org/10.1055/s-0041-1736488.

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Abstract Background Forensic odontology deals with age, sex, and race determination of the deceased at crime scenes, disasters, or natural calamities. Studies regarding the role of secondary dentin in age estimation have been documented on a several occasions in the literature but use of secondary dentin in gender predilection has not yet been documented. So, the present study was aimed at using the secondary dentin as a diagnostic tool in gender predilection. Materials and Methods The study included a total of 20 samples (10 males and 10 females). Extracted teeth were collected and placed in 10% neutral buffered formalin solution. The sections were ground on Arkansas stone until the appropriate thickness of the ground sections was achieved. The freshly prepared ground section was mounted on a slide using DPX mounting media. Ground sections were visualized under a microscope and the thickness of secondary dentine was measured using the image analysis software. Results The data were collected and subjected to group statistics. The secondary dentin thickness of all the samples, males and females, was measured. It was observed in our study that the mean thickness of secondary dentin was more in male samples (0.2970) with the standard deviation of 0.01494, as compared with that of females where the mean thickness was found to be 0.1970 with the standard deviation of 0.02058. Conclusion This study has shown that variation in secondary dentin thickness does exist between male and female groups. This criterion can be considered as an aid for gender determination in the future.
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Costa, Laís Maciel, Ariela Vilela Rizuto, Luciana Sarmento de Mendonça, Marianne de Vasconcelos Carvalho, Márcia Maria Fonseca da Silveira, and Ana Paula Veras Sobral. "Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive." Research, Society and Development 10, no. 9 (July 19, 2021): e14010917315. http://dx.doi.org/10.33448/rsd-v10i9.17315.

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The aim of the present study was to determine the expression of MMP-9, MMP-13 and TIMP-3 after using the XP Bond ™ (Dentsply) dentin-bonding agent on 21 human teeth. Class I deep cavities were prepared and restored. After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and processed for an immunohistochemical assessment (n=3). Immunohistochemical staining was performed using the monoclonal antibody anti-MMP-9, and polyclonal antibodies anti-MMP-13 and anti-TIMP-3. The immunoreactivity of metalloproteinases 9 and 13 was considered intense and moderate in the first storage intervals, before decreasing over time. In contrast, the immunoreactivity of TIMP-3 was considered absent in dentin and weak in pulp in the first time intervals, and completely absent in the subsequent intervals. MMP-9 expression in dentin and in pulp was prevalent in the dentinal tubules and odontoblastic layer, respectively. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentin and predominantly located in the odontoblastic layer for pulp. In conclusion, the expression patterns of MMP-9, MMP-13 and TIMP-3 altered in the different time intervals studied. The increased expression of TIMP-3 in the first time intervals could suggest a synchronous response to the increased expression of MMPs in the same periods. The results confirmed the low performance of TIMP-3 in the physiological processes of the pulp-dentin complex for the conditions and materials tested herein.
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Shellis, R. Peter, Michele E. Barbour, David M. Parker, Martin Addy, and Adrian Lussi. "Effects of Calcium and Phosphate on Dissolution of Enamel, Dentin and Hydroxyapatite in Citric Acid." SWISS DENTAL JOURNAL SSO – Science and Clinical Topics 133, no. 7/8 (July 10, 2023): 432–38. http://dx.doi.org/10.61872/sdj-2023-07-08-966.

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The aim was to evaluate the effect of dissolved calcium and phosphate on dissolution rate of enamel, dentin and compressed hydroxyapatite (HA) in citric acid solution as a function of pH. At pH 2.5, dissolution rate of enamel increased significantly by 6% in 20 mmol/L added calcium but, otherwise, dissolution rates of neither enamel, dentin nor HA were significantly affected by 10 or 20 mmol/L calcium. However, enamel dissolution rate was reduced by > 50 mmol/L calcium. At pH 3.25 and 4.0, 10-20 mmol/L calcium inhibited dissolution of enamel by 29-100% and HA by 65-75% but did not affect dentin dissolution. Phosphate (10 or 20 mmol/L) did not inhibit dissolution of enamel, dentin or HA at any pH, but there were increases in dissolution rate of all three substrates at pH 2.5 and, in one test with dentine (at 20 mmol/L phosphate), at pH 3.25. The results suggest that calcium addition to soft drinks and other acidic products such as medications may reduce erosivity against enamel, provided that pH is not too low; that phosphate would not reduce erosivity against enamel; and that neither calcium nor phosphate at these concentrations would reduce erosivity against dentin. Accepted for publication: December 12, 2022 Published online: February 21, 2023
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Matos, Adriana Bona, Cynthia Soares De Azevedo, Livia Tosi Trevelin, and Ana Cecília Correa Aranha. "Adhesion of a self-etching adhesive to caries-affected dentin treated with different methods for caries removal: a preliminary study." Brazilian Dental Science 19, no. 4 (November 29, 2016): 32. http://dx.doi.org/10.14295/bds.2016.v19i4.1285.

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<p><strong>Objective</strong>: The aim of this study was to evaluate the adhesion of a self-etching system to caries-affected dentin (CAD) and sound dentin (SD) after different caries removal techniques by using microtensile bond strength test (μTBS). <strong>Materials</strong> <strong>and</strong> <strong>Methods</strong>: Twenty-four extracted human molars with coronal carious lesions were used. The samples were randomly divided into 5 groups, according to caries removal method: G1 - negative control (no removal); G2 abrasive disc; G3 – slow speed steel bur; G4 - Nd:YAG laser (energy density 21.2J/cm<sup>2</sup>); G5 - Er:YAG laser (energy density 80.24J/cm<sup>2</sup>). SD and CAD/ infected dentin substrates were tested. Self-etch system (Clearfil SE Bond) was applied and resin crowns were built up. <strong>Results</strong>: One-way ANOVA showed statistically significant difference between experimental groups (p&lt;0.001), with significantly lower results for infected dentin (G1), when compared to all other experimental groups. A statistically significant higher bond strength value was observed for SD irradiated with Er:YAG laser when compared to CAD. For bur and Nd:YAG laser specimens, SD and CAD showed the same bonding performance. <strong>Conclusion</strong>: infected dentin is not an adequate substrate for bonding; Nd:YAG laser and steel burs showed the same bonding performance for both SD and CAD; while both dentins, when irradiated by Er:YAG laser, did not adequately interact with self-etching system resulting in poor adhesion.</p><p><strong>Keywords</strong>: Er:YAG laser; Nd:YAG laser; Infected dentin; Dentin.</p>
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Chen, Huang Qin, and Bin Huang. "Effect of EGCG Application on Collagen Degradation in Dentine Caries." Applied Mechanics and Materials 455 (November 2013): 112–16. http://dx.doi.org/10.4028/www.scientific.net/amm.455.112.

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Caries progress might be controlled when collagen matrix could be preserved after demineralization. The aim of this study was to investigate the effect of EGCG, a major polyphenol of green tea, on changes in loss of dry weight or solubilization of collagen from demineralized dentin sections. Dentin sections (1mm×5mm×5mm) were completely exposed to the acetic acid solution to form dentine caries. After baseline measurements of dry mass, the samples were divided into 2 groups and incubated at 37°C in either media containing EGCG or artificial saliva only for 24h, 3 days, 1 week, 2 week and 3 weeks. Following hydrolyzed with HCl, hydroxyproline (HOP) as an index of solubilization of collagen was analysed quantitatively. Both the storage medium and the storage time showed significant effects on mass loss and HOP release (p < 0.05). The incubation in EGCG resulted in relatively rapid and significant (p < 0.05) decrease in the amounts of mass loss. The HOP content of the experimental media also increased with incubation time but was significantly lower (p < 0.05) than in the control artificial saliva medium. It can be concluded that EGCG can inhibit collagen degradation and affects the further demineralization in dentin matrix, which consequently inhibits the advanced dentine caries.
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Hossain, Mozammal, Mahmood Sajedeen, and Yukio Nakamura. "Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent." Bangabandhu Sheikh Mujib Medical University Journal 12, no. 4 (December 23, 2019): 182–86. http://dx.doi.org/10.3329/bsmmuj.v12i4.43857.

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This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.
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Oliveira, Hiully Karydja Câmara, Isabela Pinheiro Cavalcanti Lima, Hugo Matheus Câmara Oliveira, Nara Grazieli Martins Lima, Talita da Silva Pinto, Matheus da Silva Regis, and Heloísa Pereira de Medeiros. "Resistência de união dos sistemas adesivos em dentina úmida e em dentina seca: revisão integrativa." Research, Society and Development 10, no. 4 (April 1, 2021): e10710413894. http://dx.doi.org/10.33448/rsd-v10i4.13894.

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A adesão à dentina é dificultada por se tratar de uma estrutura com alta composição hídrica, ao longo dos anos os protocolos clínicos, assim como os sistemas adesivos foram sendo alterados, visando melhorar a resistência de união e simplificar a técnica. Por meio de revisão integrativa, discorre-se aqui sobre a resistência de união dos sistemas adesivos em dentina úmida e em dentina seca visando sugerir melhorias dos protocolos clínicos. Selecionou-se os artigos através da biblioteca virtual em saúde (BVS), obtendo-os das bases MEDLINE, LILACS, Biblioteca Brasileira de Odontologia (BBO), além de buscas no Google® Scholar, usando os termos indexados: “Dentin”, “Dentina”, "Dentin-Bonding Agents" e “Adesivos dentinários”. Incluiu-se artigos publicados entre 2013 e 2020. Os estudos selecionados poderiam ser clínicos ou laboratoriais, em dentina úmida e em dentina seca, usando testes de resistência à tração, microtração ou cisalhamento nos estudos in vitro, com os sistemas adesivos convencionais, autocondicionantes e/ou universais. Foram excluídos artigos curtos, pôsteres ou revisões de literatura. Encontrou-se 969 artigos, sendo selecionados 8 deles, segundo os critérios de inclusão, todos os estudos abordavam o grau de umidade da dentina como um fator limitante na qualidade de adesão, assim como o conhecimento do operador a respeito da composição do sistema adesivo utilizado. Os sistemas adesivos universais foram eficazes tanto em dentina úmida quanto em dentina seca, já os sistemas adesivos convencionais foram eficientes apenas em dentina úmida.
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Jaâfoura, Sabra, Amira Kikly, Saida Sahtout, Mounir Trabelsi, and Dorra Kammoun. "Shear Bond Strength of Three Composite Resins to Fluorosed and Sound Dentine: In Vitro Study." International Journal of Dentistry 2020 (April 9, 2020): 1–7. http://dx.doi.org/10.1155/2020/4568568.

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Introduction. This in vitro study compared the shear strength of three composite resin systems to fluorosed and normal dentin. Methods. Silorane FiltekTM P90, FiltekTMZ250 XT in combination with the adhesive system AdperTM Single bond 2, and Amelogen® Plus in association with Peak Universal Bond® were tested. Fifteen normal and 15 fluorosed dentine disks were prepared per material. The shear bond strength test was performed using a universal machine. Results. One-way ANOVA revealed significant differences in bond strength between the tested composite resins. All tested materials had significantly different adhesion at the fluorosed and the nonfluorosed interface. FiltekTM Z250 XT and Silorane had lower adhesion values to fluorosed than to normal dentin. In contrast, Amelogen® Plus presented a better average resistance at the fluorosed interface. Conclusion. Amelogen® Plus presented a better average shear bond strength on the fluorosed dentine. FiltekTMZ250 XT showed the best adhesion forces and shear bond strength with sound dentine. Further studies are needed to better understand the sealing of these systems.
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Tjäderhane, Leo. "Dentin." Endodontic Topics 20, no. 1 (March 2009): 1–2. http://dx.doi.org/10.1111/j.1601-1546.2012.00273.x.

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Suzuki, Shigeki, Taduru Sreenath, Naoto Haruyama, Cherlita Honeycutt, Anita Terse, Andrew Cho, Thomas Kohler, Ralph Müller, Michel Goldberg, and Ashok B. Kulkarni. "Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization." Matrix Biology 28, no. 4 (May 2009): 221–29. http://dx.doi.org/10.1016/j.matbio.2009.03.006.

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Nasim, Iffat. "Effect of nanoparticle based intra-canal medicaments on root dentin micro-hardness." Bioinformation 18, no. 3 (March 31, 2022): 226–30. http://dx.doi.org/10.6026/97320630018226.

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Complete eradication of bacterial infection from the root canal with minimum or no effect on the microhardness of the dentin is desirable for the success of endodontic treatment. The effect of nanoparticle based intracanal medicaments on the root dentine microhardness was assessed. The medicaments used were combination of calcium hydroxide - silver nanoparticles, Combination of graphene oxide - silver nanoparticles, Calcium hydroxide and a control group. Vickers hardness value were evaluated and compared at coronal, middle and apical third level. Graphene oxide - silver nanoparticles based intracanal medicament showed least effect on the microhardness of the root dentin compared to calcium hydroxide and calcium hydroxide combined with silver nanoparticle based intracanal medicaments.
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Lee, Su Young, S. O. Jang, Hae Sun Kim, Jai Hyuk Choi, H. K. Kwon, and B. I. Kim. "Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Sodium Metasilicate." Key Engineering Materials 330-332 (February 2007): 1429–32. http://dx.doi.org/10.4028/www.scientific.net/kem.330-332.1429.

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Exposed dentine with patent tubules allows the movement of tubule fluid leading to dentine sensitivity. Occlusion of patent dentinal tubules effectively reduces the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been well known as a component of desensitizing dentifrice. Recently, new dentifrice containing sodium metasilicate was on the market for desensitizing dentifrice. The aim of this in vitro study was to compare the occlusion effect of dentinal tubule between the sodium metasilicate and the existing desensitizing dentifrices. Sixtyfour human dentine specimens were embedded into a Teflon mold and were grinded with the use of silicone carbide papers to expose the surface of dentin. The dentin surface was etched with 37% phosphoric acid for 60 seconds and was treated with ultrasonic washing for 1 hour. The dentifrices used in the study are classified into four groups: G1-dentifrice containing Sodium metasilicate, G2- dentifrice containing Strontium chloride, G3-dentifrice containing Hydroxyapatite and G4-BSI reference dentifrice (control group). Toothpaste slurries were prepared as 20 gram of toothpaste in 80 ml of distilled water. Thirty-two specimens were brushed with the toothpaste slurries using V8 Cross Brushing Machine. Tooth-brushing was performed 5,000 times using a back-and–forth stroke. The pictures of the sizes of dentinal tubules were taken by scanning electron microscopy (×3000) and changes of tubule sizes were analyzed by digital analysis. The results showed that the difference of tubule size in descending order: G1-3.30±1.80, G2-2.82±1.73, G3-1.49±1.14, G4- 1.04±0.94. The dentifrice containing Sodium metasilicate (22.5%) showed the highest dentinal tubule occlusion. Moreover, the dentifrice containing Sodium metasilicate statistically significantly increased occlusion of dentinal tubule as compared to BSI reference dentifrice (p<0.05). Thus, new desensitizing dentifrice containing sodium metasilicate was more effective than existing desensitizing dentifrice on occlusion of dentin tubules.
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Benjwal, Shrikanth, Mousumi Goswami, Aditya Saxena, Reenu Sarah Kurien, and Anam Mushtaq. "Comparative evaluation of bond strength of three self-adhering flowable composites to sound and demineralized enamel – An in vitro study." Journal of Global Oral Health 4 (June 25, 2021): 3–7. http://dx.doi.org/10.25259/jgoh_60_2020.

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Objectives: The purpose of this study was to determine bond strength of three self-adhering flowable composites. Materials and Methods: Mean tensile bond strength was measured in three groups – Group A – Constic, Group B – Dyad Flow, and Group C – Fusio Liquid Dentin on sound and demineralized primary teeth. Fracture pattern was studied using a stereomicroscope for each sample and in classified as adhesive, cohesive, or mixed fracture. Results: Mean tensile bond strength in sound enamel of Group A (Constic) was found to be 10.79 + 4.24, Group B (Dyad Flow) was 10.30 + 4.63, and of Group C (Fusio Liquid Dentine) was 11.87 + 4.45. No significant difference was found between the three groups (f = 0.327 and P = 0.724). However, a significant difference was found with demineralized enamel in three groups. Constic and Dyad Flow exhibited adhesive fracture pattern in majority of samples on sound enamel, whereas Fusio Liquid Dentin had mixed fracture pattern. On demineralized enamel, Constic exhibited adhesive fracture pattern majorly, whereas Dyad flow demonstrated mixed pattern and Fusio Liquid Dentin had more of cohesive fractures. Conclusion: Constic, Dyad Flow, and Fusio Liquid Dentin can be used instead of conventional pit and fissure sealants or in small occlusal cavities in primary teeth as a single step material.
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