Journal articles on the topic 'Delisea pulchra'

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1

Denys, R., JC Coll, and BF Bowden. "Delisea pulchra (cf. fimbriata) Revisited. The Structural Determination of Two New Metabolites From the Red Alga Delisea pulchra." Australian Journal of Chemistry 45, no. 10 (1992): 1625. http://dx.doi.org/10.1071/ch9921625.

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Two new metabolites (1a) and (2a) have been isolated from the red alga Delisea pulchra . The previously reported metabolites (3)-(7) were also isolated, and the full n.m.r. characterization of (3), (4) and (6) is reported for the first time.
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2

DE NYS, R., J. C. COLL, and B. F. BOWDEN. "ChemInform Abstract: Delisea pulchra (cf. fimbriata) Revisited. The Structural Determination of Two New Metabolites from the Red Alga Delisea pulchra." ChemInform 24, no. 6 (August 21, 2010): no. http://dx.doi.org/10.1002/chin.199306304.

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3

Dworjanyn, SA, R. de Nys, and PD Steinberg. "Chemically mediated antifouling in the red alga Delisea pulchra." Marine Ecology Progress Series 318 (August 3, 2006): 153–63. http://dx.doi.org/10.3354/meps318153.

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4

Wright, J. T., G. C. Zuccarello, and P. D. Steinberg. "Genetic structure of the subtidal red alga Delisea pulchra." Marine Biology 136, no. 3 (April 28, 2000): 439–48. http://dx.doi.org/10.1007/s002270050703.

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5

de Nys, Rocky, Anthony D. Wright, Gabriele M. König, and Otto Sticher. "New halogenated furanones from the marine alga delisea pulchra (cf. fimbriata)." Tetrahedron 49, no. 48 (January 1993): 11213–20. http://dx.doi.org/10.1016/s0040-4020(01)81808-1.

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6

Burke, Catherine, Staffan Kjelleberg, and Torsten Thomas. "Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae." Applied and Environmental Microbiology 75, no. 1 (October 31, 2008): 252–56. http://dx.doi.org/10.1128/aem.01630-08.

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ABSTRACT A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
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7

Zozaya‐Valdés, Enrique, Alexandra J. Roth‐Schulze, Suhelen Egan, and Torsten Thomas. "Microbial community function in the bleaching disease of the marine macroalgae Delisea pulchra." Environmental Microbiology 19, no. 8 (May 11, 2017): 3012–24. http://dx.doi.org/10.1111/1462-2920.13758.

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8

Dworjanyn, S. A., R. De Nys, and P. D. Steinberg. "Localisation and surface quantification of secondary metabolites in the red alga Delisea pulchra." Marine Biology 133, no. 4 (May 11, 1999): 727–36. http://dx.doi.org/10.1007/s002270050514.

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9

Rasmussen, Thomas Bovbjerg, Michael Manefield, Jens Bo Andersen, Leo Eberl, Uffe Anthoni, Carsten Christophersen, Peter Steinberg, Staffan Kjelleberg, and Michael Givskov. "How Delisea pulchra furanones affect quorum sensing and swarming motility in Serratia liquefaciens MG1." Microbiology 146, no. 12 (December 1, 2000): 3237–44. http://dx.doi.org/10.1099/00221287-146-12-3237.

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10

Maximilien, R., R. de Nys, C. Holmström, L. Gram, M. Givskov, K. Crass, S. Kjelleberg, and PD Steinberg. "Chemical mediation of bacterial surface colonisation by secondary metabolites from the red alga Delisea pulchra." Aquatic Microbial Ecology 15 (1998): 233–46. http://dx.doi.org/10.3354/ame015233.

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11

Lyons, Thérèse, Cormac GM Gahan, and Timothy P. O'Sullivan. "Structure–activity relationships of furanones, dihydropyrrolones and thiophenones as potential quorum sensing inhibitors." Future Medicinal Chemistry 12, no. 21 (November 2020): 1925–43. http://dx.doi.org/10.4155/fmc-2020-0244.

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Since their initial isolation from the marine alga Delisea pulchra, bromofuranones have been investigated as potential inhibitors of quorum sensing (QS) in various bacterial strains. QS is an important mechanism by which bacteria co-ordinate their molecular response to the environment. QS is intrinsically linked to bacterial antibiotic resistance. Inspired by nature, chemists have developed a wide variety of synthetic analogs in an effort to elucidate the structure–activity relationships of these compounds, and to ultimately develop novel antimicrobial agents. In this work, we describe advances in this field while paying particular attention to apparent structure–activity relationships. This review is organized according to the main ring systems under investigation, namely furanones, dihydropyrrolones and thiophenones.
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12

AINI, NUR, and AHMAD DWI SETYAWAN. "Bioactive compound that inhabit quorum sensing system in gram negative bacteria." Biofarmasi Journal of Natural Product Biochemistry 4, no. 1 (February 15, 2006): 34–40. http://dx.doi.org/10.13057/biofar/f040107.

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Bacteria communicate using chemical signaling molecules as words. They release, detect, and respond to the accumulation of these molecules, which are called autoinducers. Detection of autoinducers allows bacteria to distinguish between low and high cell population density, and to control gene expression in response to changes the cell number. This process is termed quorum sensing. Many bacterial behaviors are regulated by quorum sensing, including virulence factors on gram negative bacteria. Quorum sensing is a novel target for antimicrobial therapies. Many eukariots including plants, fungus, and animals produce molecules that can interfered bacteria communication, such as halogen furanon from alga Delisea pulchra, N- (heptylsulfanylacetyl)-L-homoserine-lactone from Allium sativum, and flustramine from bryozoan Flustra foliacea.
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13

Wright, JT, R. de Nys, and PD Steinberg. "Geographic variation in halogenated furanones from the red alga Delisea pulchra and associated herbivores and epiphytes." Marine Ecology Progress Series 207 (2000): 227–41. http://dx.doi.org/10.3354/meps207227.

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14

Sandy, Moriah, Jayme N. Carter-Franklin, Jessica D. Martin, and Alison Butler. "Vanadium bromoperoxidase from Delisea pulchra: enzyme-catalyzed formation of bromofuranone and attendant disruption of quorum sensing." Chemical Communications 47, no. 44 (2011): 12086. http://dx.doi.org/10.1039/c1cc15605e.

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15

DE NYS, R., A. D. WRIGHT, G. M. KOENIG, and O. STICHER. "ChemInform Abstract: New Halogenated Furanones (I) and (II) from the Marine Alga Delisea pulchra (cf. fimbriata)." ChemInform 25, no. 11 (August 19, 2010): no. http://dx.doi.org/10.1002/chin.199411276.

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16

Dahllöf, Ingela, Harriet Baillie, and Staffan Kjelleberg. "rpoB-Based Microbial Community Analysis Avoids Limitations Inherent in 16S rRNA Gene Intraspecies Heterogeneity." Applied and Environmental Microbiology 66, no. 8 (August 1, 2000): 3376–80. http://dx.doi.org/10.1128/aem.66.8.3376-3380.2000.

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ABSTRACT Contemporary microbial community analysis frequently involves PCR-amplified sequences of the 16S rRNA gene (rDNA). However, this technology carries the inherent problem of heterogeneity between copies of the 16S rDNA in many species. As an alternative to 16S rDNA sequences in community analysis, we employed the gene for the RNA polymerase beta subunit (rpoB), which appears to exist in one copy only in bacteria. In the present study, the frequency of 16S rDNA heterogeneity in bacteria isolated from the marine environment was assessed using bacterial isolates from the red alga Delisea pulchra and from the surface of a marine rock. Ten strains commonly used in our laboratory were also assessed for the degree of heterogeneity between the copies of 16S rDNA and were used to illustrate the effect of this heterogeneity on microbial community pattern analysis. The rock isolates and the laboratory strains were also used to confirm nonheterogeneity of rpoB, as well as to investigate the versatility of the primers. In addition, a comparison between 16S rDNA and rpoB PCR-DGGE (denaturing gradient gel electrophoresis)-based community analyses was performed using a DNA mixture of nine isolates from D. pulchra. Eight out of 14 isolates from D. pulchra, all rock isolates, and 6 of 10 laboratory strains displayed multiple bands for 16S rDNA when analyzed by DGGE. There was no indication of heterogeneity for either the rock isolates or the laboratory strains when rpoB was used for PCR-DGGE analysis. Microbial community pattern analysis using 16S rDNA PCR-DGGE showed an overestimation of the number of laboratory strains in the sample, while some strains were not represented. Therefore, the 16S rDNA PCR-DGGE-based community analysis was proven to be severely limited by 16S rDNA heterogeneity. The mixture of isolates from D. pulchra proved to be more accurately described using rpoB, compared to the 16S rDNA-based PCR-DGGE.
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17

de Nys, R., PD Steinberg, CN Rogers, TS Charlton, and MW Duncan. "Quantitative variation of secondary metabolites in the sea hare Aplysia parvula and its host plant, Delisea pulchra." Marine Ecology Progress Series 130 (1996): 135–46. http://dx.doi.org/10.3354/meps130135.

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18

Gram, L., R. de Nys, R. Maximilien, M. Givskov, P. Steinberg, and S. Kjelleberg. "Inhibitory Effects of Secondary Metabolites from the Red Alga Delisea pulchra on Swarming Motility of Proteus mirabilis." Applied and environmental microbiology 62, no. 11 (1996): 4284–87. http://dx.doi.org/10.1128/aem.62.11.4284-4287.1996.

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19

Fernandes, Neil, Rebecca J. Case, Sharon R. Longford, Mohammad R. Seyedsayamdost, Peter D. Steinberg, Staffan Kjelleberg, and Torsten Thomas. "Genomes and Virulence Factors of Novel Bacterial Pathogens Causing Bleaching Disease in the Marine Red Alga Delisea pulchra." PLoS ONE 6, no. 12 (December 5, 2011): e27387. http://dx.doi.org/10.1371/journal.pone.0027387.

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20

Williamson, JE, R. De Nys, N. Kumar, DG Carson, and PD Steinberg. "Induction of metamorphosis in the sea urchin Holopneustes purpurascens by a metabolite complex from the algal host Delisea pulchra." Biological Bulletin 198, no. 3 (June 2000): 332–45. http://dx.doi.org/10.2307/1542689.

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21

Fernandes, Neil, Peter Steinberg, Doug Rusch, Staffan Kjelleberg, and Torsten Thomas. "Community Structure and Functional Gene Profile of Bacteria on Healthy and Diseased Thalli of the Red Seaweed Delisea pulchra." PLoS ONE 7, no. 12 (December 3, 2012): e50854. http://dx.doi.org/10.1371/journal.pone.0050854.

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22

Harder, Tilmann, Alexandra H. Campbell, Suhelen Egan, and Peter D. Steinberg. "Chemical Mediation of Ternary Interactions Between Marine Holobionts and Their Environment as Exemplified by the Red Alga Delisea pulchra." Journal of Chemical Ecology 38, no. 5 (April 25, 2012): 442–50. http://dx.doi.org/10.1007/s10886-012-0119-5.

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23

Manefield, Michael, Martin Welch, Michael Givskov, George P. C. Salmond, and Staffan Kjelleberg. "Halogenated furanones from the red alga,Delisea pulchra, inhibit carbapenem antibiotic synthesis and exoenzyme virulence factor production in the phytopathogenErwinia carotovora." FEMS Microbiology Letters 205, no. 1 (November 2001): 131–38. http://dx.doi.org/10.1111/j.1574-6968.2001.tb10936.x.

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24

Rajasree, Odumpatta, and Arumugam Mohanapriya. "Proteome wide screening for identification of putative drug target gene in Nautella italica and structure-based ligand screening for therapeutic candidates." Research Journal of Chemistry and Environment 25, no. 12 (November 25, 2021): 122–36. http://dx.doi.org/10.25303/2512rjce122136.

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In silico based subtractive genomic approaches were employed to identify the key drug targets for an opportunistic pathogen Nautella italica, a member of the marine Roseobacter clade that causes bleaching disease in the temperate-marine red macro algae, Delisea pulchra. The aim of this study is to propose new active ligands against bleaching disease seen in algae. Using comparative and subtractive genomic approach, a set of 21 proteins were identified as the therapeutic drug target proteins for algal bleaching. This core set of drug targets has been analyzed for network topology using string network analysis and major hub gene identified by CytoHubba was rpoB (DNA directed RNA Polymerase subunit beta). The three-dimensional structure of rpoB was built by comparative modelling and used to perform a virtual screening of Zinc database by DOCK Blaster server. The 50 top scored compounds were screened for toxicity analysis by OSIRIS Data Warrior and ECOSAR tool. Further refinement by autodock program revealed two compounds ZINC49821385 and ZINC97218938 with the best binding energy of -7.07 and -6.79 respectively. These results indicated that 5-(4- isopropylphenyl)furan-2-carboxamide (ZINC ID 49821385) could be one of the potential ligand to treat bleaching disease in algae.
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25

Manefield, Michael, Rocky de Nys, Kumar Naresh, Read Roger, Michael Givskov, Steinberg Peter, and Staffan Kjelleberg. "Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein." Microbiology 145, no. 2 (February 1, 1999): 283–91. http://dx.doi.org/10.1099/13500872-145-2-283.

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26

Janssens, Joost C. A., Hans Steenackers, Stijn Robijns, Edith Gellens, Jeremy Levin, Hui Zhao, Kim Hermans, et al. "Brominated Furanones Inhibit Biofilm Formation by Salmonella enterica Serovar Typhimurium." Applied and Environmental Microbiology 74, no. 21 (September 12, 2008): 6639–48. http://dx.doi.org/10.1128/aem.01262-08.

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ABSTRACT Salmonella enterica serovar Typhimurium is a main cause of bacterial food-borne diseases. As Salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. Brominated furanones, originally extracted from the marine alga Delisea pulchra, are known to interfere with biofilm formation in several pathogens. In this study, we have synthesized a small focused library of brominated furanones and tested their activity against S. enterica serovar Typhimurium biofilm formation. We show that several furanones inhibit Salmonella biofilm formation at non-growth-inhibiting concentrations. The most interesting compounds are (Z)-4-bromo-5-(bromomethylene)-3-alkyl-2(5H)-furanones with chain lengths of two to six carbon atoms. A microarray study was performed to analyze the gene expression profiles of Salmonella in the presence of (Z)-4-bromo-5-(bromomethylene)-3-ethyl-2(5H)-furanone. The induced genes include genes that are involved in metabolism, stress response, and drug sensitivity. Most of the repressed genes are involved in metabolism, the type III secretion system, and flagellar biosynthesis. Follow-up experiments confirmed that this furanone interferes with the synthesis of flagella by Salmonella. No evidence was found that furanones act on the currently known quorum-sensing systems in Salmonella. Interestingly, pretreatment with furanones rendered Salmonella biofilms more susceptible to antibiotic treatment. Conclusively, this work demonstrates that particular brominated furanones have potential in the prevention of biofilm formation by Salmonella serovar Typhimurium.
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27

Ren, Dacheng, Laura A. Bedzyk, Peter Setlow, Dacre F. England, Staffan Kjelleberg, Stuart M. Thomas, Rick W. Ye, and Thomas K. Wood. "Differential Gene Expression To Investigate the Effect of (5Z)-4-Bromo- 5-(Bromomethylene)-3-Butyl-2(5H)-Furanone on Bacillus subtilis." Applied and Environmental Microbiology 70, no. 8 (August 2004): 4941–49. http://dx.doi.org/10.1128/aem.70.8.4941-4949.2004.

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ABSTRACT (5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) from the red marine alga Delisea pulchra was found previously to inhibit the growth, swarming, and biofilm formation of gram-positive bacteria. Using the gram-positive bacterium Bacillus subtilis as a test organism, we observed cell killing by 20 μg of furanone per ml, while 5 μg of furanone per ml inhibited growth approximately twofold without killing the cells. To discover the mechanism of this inhibition on a genetic level and to investigate furanone as a novel antibiotic, full-genome DNA microarrays were used to analyze the gene expression profiles of B. subtilis grown with and without 5 μg of furanone per ml. This agent induced 92 genes more than fivefold (P < 0.05) and repressed 15 genes more than fivefold (P < 0.05). The induced genes include genes involved in stress responses (such as the class III heat shock genes clpC, clpE, and ctsR and the class I heat shock genes groES, but no class II or IV heat shock genes), fatty acid biosynthesis, lichenan degradation, transport, and metabolism, as well as 59 genes with unknown functions. The microarray results for four genes were confirmed by RNA dot blotting. Mutation of a stress response gene, clpC, caused B. subtilis to be much more sensitive to 5 μg of furanone per ml (there was no growth in 8 h, while the wild-type strain grew to the stationary phase in 8 h) and confirmed the importance of the induction of this gene as identified by the microarray analysis.
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28

Kuehl, Richard, Sameer Al-Bataineh, Oliver Gordon, Reto Luginbuehl, Michael Otto, Marcus Textor, and Regine Landmann. "Furanone at Subinhibitory Concentrations Enhances Staphylococcal Biofilm Formation by luxS Repression." Antimicrobial Agents and Chemotherapy 53, no. 10 (July 20, 2009): 4159–66. http://dx.doi.org/10.1128/aac.01704-08.

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ABSTRACT Brominated furanones from marine algae inhibit multicellular behaviors of gram-negative bacteria such as biofilm formation and quorum sensing (QS) without affecting their growth. The interaction of furanone with QS in gram-positive bacteria is unknown. Staphylococci have two QS systems, agr and luxS, which lower biofilm formation by two different pathways, RNAIII upregulation and bacterial detachment, and polysaccharide intercellular adhesin (PIA) reduction, respectively. We synthesized natural furanone compound 2 [(5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone] from Delisea pulchra and three analogues to investigate their effect on biofilm formation in gram-positive bacteria. Compound 2, but not the analogues, enhanced the biofilms of Staphylococcus epidermidis 1457 and 047 and of S. aureus Newman at concentrations between 1.25 and 20 μM. We show the growth inhibition of S. epidermidis and S. aureus by free furanone and demonstrate bactericidal activity. An induction of biofilm occurred at concentrations of 10 to 20% of the MIC and correlated with an increase in PIA. The biofilm effect was agr independent. It was due to interference with luxS, as shown by reduced luxS expression in the presence of compound 2 and independence of the strong biofilm formation in a luxS mutant upon furanone addition. Poly(l-lysine)-grafted/poly(ethylene glycol)-grafted furanone was ineffective on biofilm and not bactericidal, indicating the necessity for free furanone. Free furanone was similarly toxic for murine fibroblasts as for staphylococci, excluding a therapeutic application of this compound. In summary, we observed a biofilm enhancement by furanone in staphylococci at subinhibitory concentrations, which was manifested by an increase in PIA and dependent on luxS.
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29

Koch, B., T. Liljefors, T. Persson, J. Nielsen, S. Kjelleberg, and M. Givskov. "The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors." Microbiology 151, no. 11 (November 1, 2005): 3589–602. http://dx.doi.org/10.1099/mic.0.27954-0.

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The function of LuxR homologues as quorum sensors is mediated by the binding of N-acyl-l-homoserine lactone (AHL) signal molecules to the N-terminal receptor site of the proteins. In this study, site-directed mutagenesis was carried out of the amino acid residues comprising the receptor site of LuxR from Vibrio fischeri, and the ability of the L42A, L42S, Y62F, W66F, D79N, W94D, V109D, V109T and M135A LuxR mutant proteins to activate green fluorescent protein expression from a PluxI promoter was measured. X-ray crystallographic studies of the LuxR homologue TraR indicated that residues Y53 and W57 form hydrogen bonds to the 1-carbonyl group and the ring carbonyl group, respectively, of the cognate AHL signal. Based on the activity and signal specificity of the LuxR mutant proteins, and on molecular modelling, a model is suggested in which Y62 (corresponding to Y53 in TraR) forms a hydrogen bond with the ring carbonyl group rather than the 1-carbonyl group, while W66 (corresponding to W57 in TraR) forms a hydrogen bond to the 1-carbonyl group. This flips the position of the acyl side chain in the LuxR/signal molecule complex compared to the TraR/signal molecule complex. Halogenated furanones from the marine alga Delisea pulchra and the synthetic signal analogue N-(sulfanylacetyl)-l-homoserine lactone can block quorum sensing. The LuxR mutant proteins were insensitive to inhibition by N-(propylsulfanylacetyl)-l-homoserine lactone. In contrast, the mutations had only a minor effect on the sensitivity of the proteins to halogenated furanones, and the data strongly suggest that these compounds do not compete in a ‘classic’ way with N-3-oxohexanoyl-l-homoserine lactone for the binding site. Based on modelling and experimental data it is suggested that these compounds bind in a non-agonist fashion.
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30

Lachnit, Tim, Torsten Thomas, and Peter Steinberg. "Expanding our Understanding of the Seaweed Holobiont: RNA Viruses of the Red Alga Delisea pulchra." Frontiers in Microbiology 6 (January 8, 2016). http://dx.doi.org/10.3389/fmicb.2015.01489.

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31

Zozaya-Valdés, Enrique, Alexandra J. Roth-Schulze, and Torsten Thomas. "Effects of Temperature Stress and Aquarium Conditions on the Red Macroalga Delisea pulchra and its Associated Microbial Community." Frontiers in Microbiology 7 (February 18, 2016). http://dx.doi.org/10.3389/fmicb.2016.00161.

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32

Gardiner, Melissa, Torsten Thomas, and Suhelen Egan. "A glutathione peroxidase (GpoA) plays a role in the pathogenicity of Nautella italica strain R11 towards the red alga Delisea pulchra." FEMS Microbiology Ecology 91, no. 4 (February 26, 2015). http://dx.doi.org/10.1093/femsec/fiv021.

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