Academic literature on the topic 'Danio rerio – Larves'
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Journal articles on the topic "Danio rerio – Larves":
Ramos, Tatiane Rogelio, Kennyson Alves de Souza, Rodrigo Augusto Cortez Passetti, Jaísa Casetta, Ana Carolina Pelaes Vital, Ricardo Pereira Ribeiro, Benício Alves de Abreu Filho, and Ivanor Nunes do Prado. "Sobrevivência de larvas de Zebrafish (Danio rerio) expostas ao extrato hidroalcoólico de Baccharis dracunculifolia." Research, Society and Development 9, no. 9 (September 1, 2020): e634997853. http://dx.doi.org/10.33448/rsd-v9i9.7853.
Silva, Niely Priscila Correia da, Tiago Queiroz da Mota Bittencourt, Thamiris Pinheiro Santos, Amanda Rodrigues dos Santos Magnabosco, Marília Cordeiro Galvão da Silva, Jadson Freitas da Silva, Paulo Eduardo da Silva Bastos, Renata Meireles Oliveira Padilha, Marilia Ribeiro Sales Cadena, and Pabyton Gonçalves Cadena. "Efeitos tóxicos de fármacos antidiabéticos como poluentes sob os parâmetros biológicos do peixe zebrafish (Danio rerio)." Revista Eletrônica Acervo Saúde 13, no. 8 (August 15, 2021): e7559. http://dx.doi.org/10.25248/reas.e7559.2021.
Rosado-Solano, Doris Natalia, Víctor Mario Jaramillo-Pérez, Vladimir Valentinovich Kouznetsov, Ricardo Restrepo-Manrique, Carlos Eduardo Puerto-Galvis, and Leonor Yamile Vargas-Méndez. "Actividad larvicida de aceites esenciales y extractos de plantas colombianas frente a Culex quinquefasciatus (Díptera: Culicidae)." ITECKNE 15, no. 2 (December 7, 2018): 79–87. http://dx.doi.org/10.15332/iteckne.v15i2.2069.
Valles, Selma, Everardo Gutiérrez López, and Ulises Bardullas. "Implementación de un sistema para evaluar la neurotoxicidad de los contaminantes ambientales en larvas de pez cebra (Danio rerio)." Investigación y Ciencia de la Universidad Autónoma de Aguascalientes, no. 74 (May 31, 2018): 25–31. http://dx.doi.org/10.33064/iycuaa2018741718.
Martins, Islane Cristina, and Pabyton Gonçalves Cadena. "ANTIEPILEPTIC DRUGS CAUSE LETHAL, SUBLETHAL, TERATOGENIC EFFECTS AND MORPHOMETRIC PARAMETERS ON EMBRYOS AND LARVAE OF ZEBRAFISH (DANIO RERIO)." Revista Ibero-Americana de Humanidades, Ciências e Educação 6, no. 12 (December 30, 2020): 485–504. http://dx.doi.org/10.51891/rease.v6i12.663.
Areias, Isabela Correa, Claudia Vianna Maurer Morelli, Viviane Cristina Fais, and Jhonathan Angel Araujo Fernandez. "Influência do ambiente enriquecido sobre respostas comportamentais no zebrafish (Danio rerio)." Revista dos Trabalhos de Iniciação Científica da UNICAMP, no. 26 (January 14, 2019). http://dx.doi.org/10.20396/revpibic262018618.
Martins, Islane Cristina. "Drogas antiepiléticas podem causar efeitos letais, subletais, teratogênicos e alterações morfométricas em embriões e larvas de zebrafish (Danio rerio)? Uma revisão sistemática." Revista Ibero- Americana de Humanidades, Ciências e Educação 7, no. 1 (2021). http://dx.doi.org/10.29327/217514.7.1-3.
Bai, Ji Dong, Youdong Wang, and Xiao-Yan Wen. "Abstract 592: Nutrigenomic Screening of Functional Foods Regulating Gluconeogenesis: A Zebrafish Pilot Study." Arteriosclerosis, Thrombosis, and Vascular Biology 34, suppl_1 (May 2014). http://dx.doi.org/10.1161/atvb.34.suppl_1.592.
Dissertations / Theses on the topic "Danio rerio – Larves":
Yedji, Rodrigue. "Perturbateurs endocriniens de type phtalate et poisson zèbre Danio rerio : approche chémoprotéomique pour l'identification des cibles et recherche de signatures d'exposition." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0106.
Phthalate esters are a family of synthetic compounds widely used as plasticisers. They are used in a number of plastic products such as packaging, toys, cosmetics, plastic roofing system and furniture decoration materials. Phthalates are not covalently bonded to the polymer matrix and are therefore easily released into the environment, resulting in animal and human exposure. In the absence of non-toxic substitutes, phthalate compounds are still widely used in industry, despite the classification of some of them by the European Chemicals Agency (ECHA) as suspected toxic substances and as endocrine disruptors. In addition, they are carcinogenic and teratogenic. The deleterious effect of phthalate esters on organisms is established, but the multiple nature of the effects observed shows that the mechanisms of action of phthalates are only partially elucidated. We used two targeted proteomics approaches to shed light on the mechanisms of action of phthalate esters. Dibutyl phthalate (DBP) was used as a model phthalate and zebrafish (D. rerio) as a model organism. Using the first targeted proteomics approach, affinity-based protein profiling (AfBPP), the functional disruption of proteins by DBP with photoaffinity probes from aryl azide synthesis was demonstrated. Optimisation of the binding conditions for diazirine probes (Diazirine 2) should provide us with a probe that can be used to identify DBP protein targets in the zebrafish proteome. The second approach, activity-based protein profiling (ABPP), used a reactive probe specific for serine hydrolases (SHs) to map active SHs in the zebrafish proteome for the first time. The identification of deregulated SHs in the presence of DBP in zebrafish larvae was also reported in this study. Overall, our results indicate that targeted proteomics approaches such as ABPP or AfBPP can be an asset for understanding xenobiotic-related mechanisms of action in ecotoxicology
Herpin, Amaury. "Caractérisation structurale et fonctionnelle de récepteurs de la superfamille des TGF-ß chez l'huître creuse Crassostrea gigas : implications évolutives." Caen, 2003. http://www.theses.fr/2003CAEN2046.
Reis, Silênio Souza. "Avaliação do desenvolvimento de embriões e larvas de zebrafish (Danio rerio) expostos ao laser de baixa potência." Universidade Federal de Goiás, 2018. http://repositorio.bc.ufg.br/tede/handle/tede/8986.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The low-power LASER is a technology used in the area of health as adjuvant therapy for various diseases. The use of conservative therapies as adjuncts to drug therapy, aim to potentiate therapeutic responses. There are currently some contraindications for the use of LASER in treatments of human patients with open wounds, tumor region and gravid uterus. Thus, zebrafish (D. rerio) is a consolidated organism as an experimental model in the biological, biomedical and environmental areas. The objective of the present work is to evaluate the development of embryos and larvae of zebrafish (D. rerio) exposed to low-power LASER. The LASER III flash with transverse beam output 0,028 cm2, power 100 mW, creep 35, 70 and 140 J / cm2, total energy of 1, 2 and 4 J (Joules), InGaAlP active medium, was used. The data were evaluated according to the normality behavior of Lilliefors using Fisher's exact test, t-test, Mann-Whitney, Kruskal-Wallis (post hoc-Dunn) and Linear Regression by the ORIGIN 7.0 program. The cumulative mortality rate and hatching in embryos (D. rerio) exposed to the LASER in the red spectrum 660 nm and infrared 830 nm 1, 2 and 4 J from 24 - 24 h to 96 hpf was not statistically significant in relation to the wavelength in energy doses in Joules (p ˃ 0,05), infrared laser 830 nm 4J, did not interfere in the development of embryos and larvae (D. rerio), when exposed from 24 - 24 h for a period of 216 hpf. The cumulative mortality rate in the control group was 23 % higher than in the LASER group and the hatching rate in the LASER group was statistically significant (p ˂ 0,05). The cumulative mortality rate in embryos and larvae (D. rerio) when exposed intensively to LASER was statistically significant, as was the hatching rate (p ˂ 0,05) for 96 hpf. There were no statistically significant differences for larval length between the control group compared to the LASER group 24 - 24 h and intensive LASER (p ˃ 0,05). The rate of heart rate in the LASER group 24 - 24 h was statistically significant in relation to the control group and the intensive LASER group (p ˂ 0,05). The rate of spontaneous movements was statistically significant when compared to the control group and the intensive LASER group (p ˂ 0,05). The rate of edema in the control group was statistically significant in relation to the LASER group 24 - 24 h for 96 hpf (p ˂ 0,05). The rate of edema in the intensive LASER group was not statistically significant; however, as in the LASER group 24 - 24 h, the edema in the control group was higher than in the LASER group. The scarcity of theoretical reference on this theme reinforces the pioneering of this work carried out in vivo. The results of this study highlight the importance of the use of embryos and larvae (D. rerio) as a model system in biological, environmental and biomedical research. Data analysis suggests that contraindications for low-power LASER irradiation in patients during the gestational period should be reviewed.
O LASER de baixa potência é uma tecnologia utilizada na área da saúde como terapia coadjuvante a diversas doenças. A utilização de terapias conservadoras como coadjuvantes a terapia medicamentosa, visam potencializar as repostas terapêuticas. Atualmente há algumas contraindicações para utilização do LASER em tratamentos de pacientes humanos com feridas abertas, região tumoral e útero gravídico. Sendo assim, o zebrafish é um organismo consolidado como modelo experimental nas áreas biológica, biomédica e ambiental. O objetivo do presente trabalho é avaliar o desenvolvimento de embriões e larvas do zebrafish (D. rerio) expostos ao LASER de baixa potência. Utilizou-se o flash LASERIII com saída transversa do feixe 0,028 cm2, potência 100 mW, fluência 35, 70 e 140 J/cm2, energia total de 1, 2 e 4 J(Joules), meio ativo InGaAlP. Os dados foram avaliados segundo o comportamento de normalidade Lilliefors através dos testes Exato de Fisher, teste t, Mann-Whitney, Kruskal-Wallis (post hoc-Dunn) e Regressão Linear pelo programa ORIGIN 7.0. A taxa de mortalidade acumulativa e eclosão em embriões (D. rerio) expostos ao LASER no espectro vermelho 660 nm e infravermelho 830 nm 1, 2 e 4 J de 24 - 24 h para 96 hpf não foi estatisticamente significativas em relação ao comprimento de onda em doses de energia em Joules (p ˃ 0,05), O LASER infravermelho 830 nm 4J, não interferiu no desenvolvimento de embriões e larvas (D. rerio), quando expostos de 24 - 24 h por um período de 216 hpf. A taxa de mortalidade acumulativa no grupo controle foi 23 % maior que no grupo LASER e a taxa de eclosão no grupo LASER foi estatisticamente significativa (p ˂ 0,05). A taxa de mortalidade acumulativa em embriões e larvas (D. rerio) quando expostos de forma intensiva ao LASER, foi estatisticamente significativa, assim como a taxa de eclosão (p ˂ 0,05) para 96 hpf. Não houve diferenças estatisticamente significativas para comprimento larval entre o grupo controle em relação ao grupo LASER 24 - 24 h e LASER intensivo (p ˃ 0,05). A taxa de batimentos cardíacos no grupo LASER 24 - 24 h foi estatisticamente significativo em relação ao grupo controle e ao grupo LASER intensivo (p ˂ 0,05). A taxa de movimentos espontâneos foi estatisticamente significativa, quando comparada ao grupo controle e ao grupo LASER intensivo (p ˂ 0,05). A taxa de edema no grupo controle foi estatisticamente significativa em relação ao grupo LASER 24 - 24 h para 96 hpf (p ˂ 0,05). A taxa de edema no grupo LASER intensivo não foi estatisticamente significativa, no entanto, assim como no grupo LASER 24 - 24 h, o edema no grupo controle foi maior que no grupo LASER. A escassez de referencial teórico sobre essa temática reforça o pioneirismo desse trabalho realizado in vivo. Os resultados desse estudo ressaltam a importância da utilização de embriões e larvas (D. rerio) como Sistema - modelo em pesquisas biológicas, ambientais e biomédicas. As análises dos dados sugerem que as contraindicações para irradiação com o LASER de baixa potência em pacientes durante o período gestacional sejam revistas.
Fenero, Camila Ideli Morales. "Estudo do modelo de inflamação intestinal induzida por TNBS em larvas de Zebrafish (Danio rerio)." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-09122015-123622/.
Inflammatory bowel diseases are characterized by a dysregulation in immune response against microbiota. The zebrafish has emerged as a new model for the study of inflammatory diseases. Short chain fatty acids (SCFAs) are products of the intestinal microflora, which have anti-inflammatory roles and appear as a possible therapy against inflammatory diseases. The implementation of intestinal inflammation model induced by trinitrobencenesulfonic acid (TNBS) in zebrafish larvae, increased mortality in a dose-dependent manner. Was observe dilatation lumen and straightening of the intestinal villi. No changes were detected in the production of mucus or in the number of goblet cells but had a slight increase in apoptosis and decreased proliferation in exposed to TNBS. Also has, increased cytokines and infiltrating myeloid cells, and dysbiosis of the microbiota. Treatment with SCFAs generate high mortality above 10 mM concentrations, but there anti-inflammatory action to this same concentration.
Soares, J?ssica Cavalheiro. "Efeitos neurot?xicos da exposi??o ao ?xido de grafeno em larvas de zebrafish (Danio rerio)." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7519.
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Recent studies have shown several applications of graphene oxide (GO) in neurology in the imaging area as a contrast agent for diagnostic purposes, for drug delivery of anticarcinogenic drugs, proteins and peptides and in the field of tissue engineering for applications in the regeneration of nervous lesions. Advances in GO applications research in neurology require addressing its potential toxic mechanisms of action. Zebrafish (Danio rerio) has been widely used in neurotoxicological trials due to the homology of biochemical processes and neural structures with vertebrates. To investigate the potential neurotoxic effects of GO exposure in different concentrations at development period, we evaluated in vivo parameters for initial toxicological screening. We found that GO did not induce changes in survival, hatching and spontaneous movement. However, an increase in heart rate at 48 hpf was found and a reduction in body length without altering the ocular area at 5 dpf. Additionally, molecular gene expression analyses of nervous system-related proteins were performed, showing synapsin IIa expression is increased and dopamine transporter (dat) gene expression is reduced, suggesting a potential compensatory mechanism. The evaluation of the locomotion behavior of animals exposed at GO showed an increase in the absolute turn angle. Based on these initial parameters, we performed biochemical analyzes to determination of the enzyme acetylcholinesterase (AChE) activity and dopamine levels. The exposure at GO did not change AChE activity and decreased dopamine levels. Additionally, the gene expression of B cell lymphoma 2 (bcl2) gene and caspase 3 (casp3) gene were evaluated for the group of larvae exposed to GO, demonstrating an increase of bcl2 and unchanged casp3 expression, suggesting no apoptosis involvement. The tissue and cellular structure of zebrafish larvae brain exposed at GO was evaluated by transmission electron microscopy (TEM) and cell dead by autophagosome formation with loss of cellular architecture was observed most likely due to exposure to the nanomaterial. Further studies are necessary to the complete understanding of the neurological changes observed in zebrafish by exposure to GO and the mechanisms involved in this process, are necessary.
Estudos recentes t?m mostrado diversas aplica??es do ?xido de grafeno (GO) como agente de contraste para fins de diagn?stico, para o endere?amento de drogas (do ingl?s drug delivery) de f?rmacos anticarcinog?nicos, prote?nas e pept?deos e na ?rea de engenharia tecidual para aplica??es na regenera??o de les?es nervosas. O avan?o em pesquisas relacionadas a aplica??es do GO em neurologia exige a investiga??o dos mecanismos de a??o potencialmente t?xicos desencadeados pela exposi??o ao nanomaterial. O zebrafish (Danio rerio) tem sido amplamente utilizado em ensaios neurotoxicol?gicos devido ? homologia de processos bioqu?micos e estruturas neurais com outros vertebrados, vis?veis desde o in?cio de seu desenvolvimento externo. Para investigar os efeitos neurot?xicos da exposi??o a diferentes concentra??es do GO no per?odo de desenvolvimento, foram avaliados diversos par?metros in vivo para avalia??o toxicol?gica preliminar. Foi verificado que o GO n?o induziu altera??es na sobreviv?ncia, eclos?o e movimentos espont?neos. Contudo, o aumento dos batimentos card?acos em 48 hpf foi constatado e redu??o do tamanho do eixo axial, sem altera??o na ?rea ocular em 5 dpf. Adicionalmente, an?lises moleculares da express?o de genes espec?ficos para prote?nas do sistema nervoso foram realizadas mostrando que o GO aumenta a express?o de sinapsina IIa e reduz a express?o do transportador de dopamina (dat), sugerindo um prov?vel mecanismo compensat?rio. A avalia??o da locomo??o dos animais expostos ao GO mostra o aumento do ?ngulo absoluto de virada. Com base nestes par?metros iniciais, foram realizadas an?lises bioqu?micas para a determina??o da atividade da enzima acetilcolinesterase (AChE) e dosagem dos n?veis de dopamina. A exposi??o ao GO n?o alterou a atividade da AChE e reduziu os n?veis de dopamina. Adicionalmente, a express?o do gene de linfoma de c?lulas B2 (do ingl?s B cell lymphoma 2 (bcl2) ) e do gene que codifica para caspase 3 (casp3) foram avaliadas para o grupo de larvas expostas ao GO onde foi mostrado aumento da express?o de bcl2, enquanto que a express?o de casp3 permaneceu inalterada, sugerindo que o GO parece n?o induzir mecanismo de apoptose. A estrutura tecidual e celular do c?rebro de larvas de zebrafish expostas ao GO foi avaliada por microscopia eletr?nica de transmiss?o (TEM) onde morte celular por necrose com perda da arquitetura celular foi constatada. Estudos adicionais para o completo entendimento das altera??es neurol?gicas observadas pela exposi??o ao GO e dos mecanismos envolvidos nesse processo s?o necess?rios.
SILVA, Lívia Laís de Santana. "Avaliação da toxicidade de extrato e lectina de sementes de Moringa oleifera (WsMol) sobre larvas de Danio rerio (peixe paulistinha)." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/25860.
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CAPES
A WSMoL (do inglês water-soluble Moringa oleifera lectin) é uma lectina (proteína que reconhece carboidratos) proveniente das sementes da Moringa oleifera, as quais são utilizadas no tratamento de água. Algumas espécies de insetos podem atuar como vetores de doenças ou pragas agrícolas. O mosquito Aedes aegypti é o vetor da dengue e sabe-se que o extrato de sementes de M. oleifera e WSMoL são tóxicos para as larvas deste inseto. O extrato de sementes e WSMoL apresentaram uma concentração letal a 50% das larvas após 24 horas de exposição (CL₅₀) igual a 0,27 mg/mL e 0,197 mg/mL, respectivamente. Entretanto, para uma adequada utilização desse inseticida natural se faz necessário a realização de testes ecotoxicológicos a fim de avaliar os possíveis efeitos causados em organismos aquáticos não-alvo. Neste trabalho, foi avaliada a toxicidade de extrato de semente e WSMoL para larvas de Danio rerio, espécie de peixe muito utilizada na Ecotoxicologia devido à facilidade de observação de seu comportamento e desenvolvimento e serem sensíveis na fase larval. As larvas de D. rerio foram expostas a diferentes concentrações do extrato de sementes ou WSMoL por 168 horas (7 dias),tendo sido avaliados efeitos letais por meio da determinação da taxa de mortalidade. Além disso, efeitos sub-letais comportamentais e bioquímicos sobre a velocidade natatória das larvas e atividade da acetilcolinesterase, respectivamente,também foram avaliados. Foi observado um efeito dose dependente na taxa de mortalidade de D. rerio tanto para o extrato quanto para a lectina purificada. Após 24 horas, os valores de CL₅₀/₂₄h foram 0,365 e 0,21 mg/mL para extrato e WSMoL, respectivamente. Esses resultados mostram que a susceptibilidade das larvas de D. rerio a WSMoL foi similar a das larvas de A. aegypti (6% de diferença nos valores de LC₅₀/₂₄h) enquanto extrato é aproximadamente 34% mais potente para as larvas do mosquito. Após 96 horas, os valores de CL₅₀/₂₄h para extrato e WSMoL foram 0,031 e 0,135 mg/mL, respectivamente. Com relação à velocidade natatória dos indivíduos expostos ao extrato, houve um aumento significativo (p < 0,05) após incubação nas concentrações 0,03 e 0,27 mg/mL e uma diminuição para larvas incubadas com 0,81 mg/mL por esse mesmo período. Para incubação por 168 h, a velocidade foi maior que no grupo controle para os tratamentos a 0,27 e 0,81 mg/mL. Nos ensaios com WSMoL, a média da velocidade de natação de larvas expostas à lectina por 72 h foi significativamente (p < 0,05) menor que no controle nos tratamentos com 0,1 e 0,2 mg/mL. Para 168 h, a velocidade foi também menor que no controle no tratamento a 0,1 mg/mL enquanto para as concentrações de 0,02 e 0,05 mg/mL foi similar (p > 0,05) ao controle.Larvas expostas por 168 h ao extrato aquoso na concentração 0,81 mg/mL e a WSMoL na concentração de 0,1 mg/mL apresentaram uma redução significativa na atividade da acetilcolinesterase. Levando em consideração os resultados obtidos, esses inseticidas naturais devem ser usados cuidadosamente, uma vez que as concentrações necessárias para o controle de A. aegyptiforam podem também causar efeitos tóxicos letais e sub-letais para organismos não-alvo como o peixe D. rerio. Recomenda-se o uso em recipientes de armazenamento de água, e não em ecossistemas aquáticos como lagoas e lagos.
Some insect species can act as vectors of diseases or as agricultural pests. The WSMoL (water soluble Moringa oleifera lectin) is a lectin (protein that recognizes carbohydrate) derived from the seeds of Moringa oleifera, which are used in water treatment. The mosquito Aedes aegypti is the vector of dengue and it is known that M. oleifera seed extract and WSMoL are toxic to larvae of this insect. The seed extract and WSMoL showed a lethal concentration to 50% of the larvae after 24 hours of exposure (LC₅₀) equal to 0.27 mg/mL and 0.197 mg/mL, respectively. However, for proper use of these natural insecticides, it is necessary to carry out ecotoxicological tests in order to assess the possible effects on non-target aquatic organisms. In this work, the toxicity of seed extract and WSMoL to zebrafish (Danio rerio) larvae was evaluated. Danio rerio is a fish species very used in Ecotoxicology due to the ease of observation of its behavior and development and for being sensitive in the larval stage. D. rerio larvae were exposed to different concentrations of the seed extract or WSMoL for 168 h (7 days) and the lethal effects were assessed by determining the mortality rate. Additionally, behavioral and biochemical sublethal effects on larval swimming velocity and acetylcholinesterase activity, respectively, were also evaluated. It was observed a dose dependence effect on D. rerio mortality rate for both the extract and the purified lectin. After 24 h, the values of LC₅₀/₂₄h were 0.365 and 0.21 mg/mL to extract and WSMoL, respectively. These results show that the D. rerio larvae susceptibility to the WSMoL was similar to A. aegypti larvae (6% difference in LC₅₀/₂₄h values) while extract was about 34% more potent against mosquito larvae. After 96 h, the values of LC₅₀/₂₄h for extract and WSMoL were 0.031 and 0.135 mg/mL, respectively. Concerning the swimming velocity of individuals exposed to the extract there was a significant increase (p <0.05) after incubation for 72 h at 0.03 and 0.27 mg/mL and a decrease for larvae incubated at 0.81 mg/mL for this same period. For 168 h incubation, the velocity was higher than control group for treatments at 0.27 and 0.81 mg/mL. In tests with WSMoL, the average swimming velocity of larvae exposed to the lectin for 72 h was significantly (p <0.05) lower than the control on treatments with 0.1 and 0.2 mg/mL. For 168 h, the velocity was also lower than the control on treatment with 0.1 mg/mL, and with concentrations of 0.02 and 0.05 mg/mL the values were similar (p>0.05) to the control. Larvae exposed for 168 h to the aqueous extract at a concentration of 0.81 mg/mL and WSMoL at 0.1 mg/mL showed a significant reduction in the activity of acetylcholinesterase. Considering the results, these natural insecticides must be used carefully used since the concentrations necessary for the control of A. aegypti also can cause lethal toxic effects and sub-lethal to non-target organisms such as D. rerio fish. It is not recommended to use these natural insecticides in aquatic ecosystems such as ponds and lakes, but in water storage containers.
Gore, Matthew R. "Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio)." Thesis, University of North Texas, 2007. https://digital.library.unt.edu/ark:/67531/metadc3669/.
Nogueira, Ana Filipa Ferreira. "Developmental, behavioral, biochemical and epigenetic effect of pharmaceuticals in larvae and embryos of Danio rerio." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22024.
The scientific community has been concerned for several years about the presence of pharmaceuticals in the wild, since these compounds may have deleterious or unpredictable effects on living organisms. The scientific knowledge on the fate and effect of drug residues in the environment has evolved considerably in recent years, revealing that drug residues do indeed pose a threat to the aquatic ecosystem. Two examples of widely used pharmaceuticals that are present in the environment are paracetamol (acetaminophen) and ciprofloxacin, Paracetamol is a drug with analgesic properties, used for the temporary relief of mild to moderate pain associated with common colds, and in the reduction of fever. Ciprofloxacin is an antibiotic from the chemical class of the fluoroquinolones which presents a broad antibacterial spectrum. Firstly we decided the range of concentrations for each drug. In the case of paracetamol, the tested concentrations were 0.005 mg/L, 0.025 mg/L, 0.125 mg/L, 0.625 mg/L, and 3.125 mg/L, plus a control treatment with water from the facility system. And for ciprofloxacin, the tested concentrations were 0.005 μg/L, 0.013 μg/L, 0.031 μg/L, 0.078 μg/L, 0.195 μg/L, and 0.488 μg/L. These concentrations were chosen as for their environmental relevance since they are close to the real concentrations of these pharmaceuticals found in surface waters and effluents. This work aims to characterize the effects of both drugs in zebrafish embryos and larvae, not only in developmental and behaviour parameters, but also using a biomarker-based approach, namely by quantifying the activities of catalase (CAT), glutathione-S-transferase (GST’s), cholinesterases (ChE’s), glutathione peroxidase (GPx), and determining the TBARS level. Exposure to paracetamol caused an increase in the percentage of organisms with morphological deformations, but no morphological deformations were observed in organisms exposed to ciprofloxacin. Concerning larvae behavioural tests, significant differences were observed for larvae exposed to paracetamol but not for larvae exposed to ciprofloxacin. In the biomarker determination, both drugs caused a statistically significant increase in ChE activity; in CAT only ciprofloxacin caused a significant difference, a decrease in its activity. Paracetamol induced an increase in activity for total GPx and in GST’s. The TBARS levels significantly increased in the exposure to paracetamol, but they significantly decreased in the exposure to ciprofloxacin. In this work, we also prepared an immunohistochemical detection of global methylation, which allowed to observe that, in embryos, the highest concentration of paracetamol caused a slight increase in the intensity of the 5- mdC signal, which can be translated into a slight increase in the DNA methylation. On the other hand, the embryos exposed to ciprofloxacin did not appear to have any difference from control. The comparison of the here-obtained results for the two different drugs allows observing that zebrafish larvae were more sensitive to the exposure to paracetamol, being the most sensitive methodology the biomarker determination. We can also conclude that oxidative stress occurred as a consequence of the exposure to both pharmaceuticals, being more evident in the case of paracetamol. We also showed that the oxidative stress created by the two pharmaceuticals may be the cause of all the other observations.
A presença de produtos farmacêuticos na natureza é uma questão emergente para a comunidade científica uma vez que estes compostos podem ter efeitos deletérios ou imprevisíveis sobre os organismos vivos. O estudo dos efeitos dos resíduos de medicamentos no ambiente evoluiu consideravelmente nos últimos anos, revelando que estes resíduos são uma ameaça para o ecossistema aquático. Dois exemplos de produtos farmacêuticos amplamente utilizados por humanos e presentes no meio ambiente são o paracetamol (acetaminofeno) e a ciprofloxacina. O paracetamol é um medicamento com propriedades analgésicas, utilizado para o alívio temporário da dor leve a moderada associada a gripes comuns, e na redução da febre. A ciprofloxacina é um antibiótico da classe química das fluoroquinolonas que apresenta um amplo espectro antibacteriano. Este trabalho tem como objetivo utilizar larvas de peixe-zebra e caracterizar os efeitos de ambos os medicamentos, não apenas nos parâmetros de desenvolvimento e comportamento, mas também usando com uma abordagem baseada em biomarcadores, nomeadamente quantificando as atividades de catalase (CAT), glutationa-S-transferase (GST), colinesterases (ChE's), glutationa peroxidase (GPx) e determinação do nível TBARS, bem como uma abordagem epigenética. Neste trabalho, também avaliamos por técnicas histoquímicas a metilação global do ADN. No caso do paracetamol, as concentrações testadas foram 0.005 mg/L, 0.025 mg/L, 0.125 mg/L, 0.625 mg/L, e 3.125 mg/L, juntamente com o controlo que apenas continha água do sistema. Para a ciprofloxacina, as concentrações testadas foram 0.005 μg/L, 0.013 μg/L, 0.031 μg/L, 0.078 μg/L, 0.195 μg/L, e 0.488 μg/L. Estas concentrações foram escolhidas pela sua relevância ambiental, uma vez que são muito próximas às concentrações destes fármacos no ambiente. A exposição ao paracetamol causou um aumento na percentagem de organismos com deformações morfológicas, mas não foram observadas deformações morfológicas em organismos expostos à ciprofloxacina. Quanto aos testes comportamentais com larvas de peixe-zebra, observaram-se diferenças significativas nas larvas expostas ao paracetamol, que demonstraram uma maior distância de natação, mas o mesmo efeito não foi observado para as larvas expostas à ciprofloxacina. Os dados obtidos para os diferentes biomarcadores demonstraram que ambos os fármacos causaram um aumento estatisticamente significativo na atividade de ChE. Na actividade da CAT, apenas a ciprofloxacina causou diferença significativa, mais propriamente uma descida na atividade da CAT. O paracetamol induziu um aumento na atividade da GPx total e das GSTs. Os níveis de TBARS aumentaram significativamente após exposição ao paracetamol, mas diminuíram significativamente em organismos expostos à ciprofloxacina. Os dados relativos à quantificação da metilação global do ADN permitiram observar que, em embriões, a maior concentração de paracetamol causou um ligeiro aumento na intensidade do sinal do marcador de metilação de ADN (5- mdC,) quando observado por miscroscopia de confocal. Por outro lado, os embriões expostos à ciprofloxacina não aparentavam ter qualquer diferença no perfil deste marcador de epigenética comparativamente ao controlo. A comparação dos resultados aqui obtidos para os dois fármacos diferentes permite observar que as larvas de peixe zebra eram mais sensíveis à exposição ao paracetamol, sendo a metodologia mais sensível a determinação do biomarcador. Também podemos concluir que o estresse oxidativo ocorreu como conseqüência da exposição a ambos os produtos farmacêuticos, sendo mais evidente no caso do paracetamol. Também mostramos que o estresse oxidativo criado pelos dois produtos farmacêuticos pode ser a causa de todas as outras observações.
Perrichon, Prescilla. "Développement de tests embryonnaires prédictifs d’effets toxiques précoces et tardifs pour des molécules hydrophobes." Thesis, La Rochelle, 2014. http://www.theses.fr/2014LAROS040/document.
PAHs are ubiquitous widespread contaminants which emissions are overgrowing with increasing anthropogenic activities. These semi-persistent chemicals are threatening organisms in the environment. Ecosystems health and resilience are essential to life and societal functioning. Impact assessment of these chemicals is a real requirement for society thereby establishing the european legislative (DCE, Water Framework Directive) and regulations (REACH, Registration, Evaluation, Authorisation and Restriction of Chemicals) for better (eco)toxicological risk management. In this context, many bioassays have been developed to assess environmental quality, the toxicity of chemicals (including mixture) on organisms and its underlying mechanisms. This study aimed to identify (eco)toxicological risks in the context of fish embryo-larval assay, using a relevant and well-known model : the zebrafish Danio rerio. Two complementary approaches (chemical and biological) were used to assess transfer, fate and toxicity of PAHs. Induced-responses were evaluated at different levels of biological organization, from molecular (oxidative stress, DNA damage, EROD) to physiological (cardiac activity), behavioral (PhotoMotor Response) and morphological levels. Among the three exposure routes tested, the sediment contact exposure was not suitable for PAHs toxicity assessment. In contrast, exposures to water-accommodated fractions (WAF) of petroleum products represented a more reproducible, sensitive and integrative approach for testing multiscale toxic effects. Following these experiments, the observation scope of induced effects should be broadened beyond the standard duration recommended by the Organization for Economic Cooperation and Development in order not to underestimate the acute effect of the tested-compounds. Furthermore, our multigenerational study showed physiological and behavioral disturbances on the first generation of offspring providing from parents exposed to contaminated-food with three aromatic extracts pyrolytic and petrogenic origin (heavy and light). Although the contamination transfer of PAHs has not been revealed, the observed alterations (probably due to a transfer through genetic and epigenetic modifications) in the early stages of zebrafish could have adverse effects on survival and recruitment populations. Multigenerational studies prove to be an integrated approach for the toxicity assessment of chemicals and strengthen the predictive effects. These ecotoxicological studies should be widely undertaken to evaluate the potential for exposed-population to maintain in the future time
Delomas, Thomas Allin. "Sex determination and interspecies hybridization in zebrafish Danio rerio and pearl danio D. albolineatus." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1522574215273393.
Book chapters on the topic "Danio rerio – Larves":
Schilling, Thomas F. "The morphology of larval and adult zebrafish." In Zebrafish, 59–94. Oxford University PressOxford, 2002. http://dx.doi.org/10.1093/oso/9780199638086.003.0004.
Cristina Pereira, Lilian, Paloma V.L. Peixoto, and Cristina Viriato. "Application of Zebrafish in Mitochondrial Dysfunction." In Zebrafish Research - An Ever-Expanding Experimental Model. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1003967.
Conference papers on the topic "Danio rerio – Larves":
Ridlayanti, Annisa, Aida Ratna Wijayanti, Husnul Khotimah, and Muljohadi Ali. "Protection of Centella asiatica Extract Through BDNF Expression on Stunting Model Zebrafish Larvae (Danio rerio) by Rotenone Induced." In 1st Paris Van Java International Seminar on Health, Economics, Social Science and Humanities (PVJ-ISHESSH 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210304.170.
Primaditya, Vanda, Fitra Arsy Nur Cory’ah, Linda Ika Puspita Ariati, Zakiah, Dyah Woro Kartiko Kusumo Wardani, Yuningsih, Dianita Primihastuti, Husnul Khotimah, Mohammad Muljohadi Ali, and Wibi Riawan. "Effect of Centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model." In PROCEEDINGS OF THE 3RD INTERNATIONAL SEMINAR ON METALLURGY AND MATERIALS (ISMM2019): Exploring New Innovation in Metallurgy and Materials. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0002607.
Li, Gen, Hao Liu, Ulrike K. Mu¨ller, and Johan L. van Leeuwen. "Swimming Hydrodynamics and Maneuverability in C-Start of Zebrafish Larvae: An Integrated Computational Study." In ASME-JSME-KSME 2011 Joint Fluids Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajk2011-19020.
Salazar Moscoso, Marcela, Silvia Joly Ruiz Castellanos, Guillem Anglada Escudé, and Laia Ribas Cabezas. "Hypergravity induces changes in physiology, gene expression and epigenetics in zebrafish." In Symposium on Space Educational Activities (SSAE). Universitat Politècnica de Catalunya, 2022. http://dx.doi.org/10.5821/conference-9788419184405.044.