Dissertations / Theses on the topic 'Cytoskeleton'
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Morgan, Rachel E. "Is the Cytoskeleton Necessary for Viral Replication?" Digital Archive @ GSU, 2012. http://digitalarchive.gsu.edu/biology_theses/38.
Full textMcDermott, Joshua D. "The ovine lens cytoskeleton." Lincoln University, 2007. http://hdl.handle.net/10182/700.
Full textSnyder, Heidi Ghent. "Fiber type-specific desmin content in human single muscle fibers /." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1253.pdf.
Full textFawell, E. H. "Studies on the microvillus cytoskeleton." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355700.
Full textSchneider, André. "The cytoskeleton of Trypanosoma brucei /." [S.l.] : [s.n.], 1988. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Full textMcCarthy, David James. "Analysis of the novel Lyn-associated cytoskeletal modular protein, LACM." University of Western Australia. School of Medicine and Pharmacology, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0180.
Full textTharmann, Rainer. "Mechanical properties of complex cytoskeleton networks." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=97998002X.
Full textBrown, Jennifer. "Investigating the actin cytoskeleton in cancer." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7266/.
Full textBalanda, Matthew L. "The use of cytoskeletal inhibitors to determine the role of the cytoskeleton in the activation of hypertonicity-induced currents in xenopus oocytes /." View abstract, 1999. http://library.ctstateu.edu/ccsu%5Ftheses/1561.html.
Full textThesis advisor: Kathy Martin. " ... in partial fulfillment of the requirements for the degree of Master of Arts in Biological Sciences." Includes bibliographical references (leaves 42-44).
Huber, Florian. "Emergent structure formation of the actin cytoskeleton." Doctoral thesis, Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-86666.
Full textBoey, Seng Kee. "Computer simulation of the human erythrocyte cytoskeleton." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24296.pdf.
Full textNuydens, Rony Maria. "Dynamics of the neuronal cytoskeleton during apoptosis." Maastricht : Maastricht : Universiteit Maastricht ; University Library, Maastricht University [Host], 1999. http://arno.unimaas.nl/show.cgi?fid=6860.
Full textFörster, Florian. "Targeting the actin cytoskeleton with natural compounds." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-168914.
Full textKim, Taeyoon Ph D. Massachusetts Institute of Technology. "Simulation of actin cytoskeleton structure and rheology." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/39875.
Full textIncludes bibliographical references (p. 81-87).
Structures consisting of G-actin or other filament-forming monomers show a variety of morphologies with widely different properties in regard to pore size, degree of isotropy, and extent of cross-linking. These characteristics are primarily determined by the concentration and feature of proteins which cross-link filaments, but little is known how the filament-forming monomers and cross-linking proteins are organized in order to produce various network morphologies. In addition, it's generally known that mechanical force plays an important role in the physiology of eukaryote cells whose major structural component in cortex is actin cytoskeleton. Thus, understanding the origin of viscoelasticity of cross-linked networks should be crucial to figure out the exact role of cytoskeletal behaviors in many cellular functions. Here, we introduce a Brownian dynamics (BD) simulation model in three dimensions in which actin monomers polymerize into a filament and become cross-linked by two types of cross-linking molecules that constitute either perpendicular or parallel cross-links. We evaluate the influences of system parameters on the morphology of resultant networks. Some scaling behaviors that are independent of the specific choice of most parameters appear.
(cont.) Additionally, the modified model is employed to investigate the viscoelastic property of actin-like network by tracking the trajectories of filaments. This method is theoretically more direct and more precise than micro-bead rheology used in experiments. The viscoelastic property appears to be highly affected by characteristics of cross-linking molecules, average filament length, and concentration of actin monomers. Our model has the high potential as a BD model that can be applied for investigating a variety of actin-related phenomena after further refinement and modification.
by Taeyoon Kim.
S.M.
Ju, M. "Role of membrane cytoskeleton in fenestra biogenesis." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1383777/.
Full textDobbins, G. Clement. "The role of the cytoskeleton in AChR clustering." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2007p/dobbins.pdf.
Full textEsson, Heather Jean. "Morphological evolution and development of the euglenid cytoskeleton." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/6814.
Full textAttaran, Amir. "CTL cytotoxicity and the cytoskeleton : a microscopical study." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308607.
Full textCarballido-Lopez, Rut. "Bacterial cytoskeleton : cell shape determination in Bacillus subtilis." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270007.
Full textCorben, Elizabeth Bower. "An immunological approach to the higher plant cytoskeleton." Thesis, University of East Anglia, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540595.
Full textCain, R. J. "Manipulation of the eukaryotic cytoskeleton by invasive Salmonella." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597214.
Full textAdvani, Andrew. "The blood cell cytoskeleton in type 2 diabetes." Thesis, University of Newcastle Upon Tyne, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289211.
Full textFeret, Dorota. "Proteasomal control of the fission yeast microtubule cytoskeleton." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704739.
Full textSpeldewinde, Shaun. "Prions, autophagy, ageing and actin cytoskeleton in yeast." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/prions-autophagy-ageing-and-actin-cytoskeleton-in-yeast(03085d7f-283a-40e1-bcf7-d9533ff2e2fc).html.
Full textUppal, Sonal. "Studies of Microtubule Inhibitor Combinations on Cytoskeleton Architecture." Bowling Green State University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1162842626.
Full textTurner, Jerrold Ross. "Secretory organelles and the cytoskeleton: Organization and interdependence." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054668919.
Full textKumar, Sanjay. "Molecular mechanisms of organization in the neurofilament cytoskeleton." Available to US Hopkins community, 2002. http://wwwlib.umi.com/dissertations/dlnow/3080703.
Full textRicci, Mario. "The marsupial sperm tail cytoskeleton : a morphological and biochemical study /." Title page, table of contents and abstract only, 2004. http://web4.library.adelaide.edu.au/theses/09PH/09phr4911.pdf.
Full textTurina, Dean. "Propofol changes the cytoskeletal function in neurons : An experimental study in cortical cultures." Doctoral thesis, Linköpings universitet, Anestesiologi med intensivvård, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-77219.
Full textJopson, Martin Frederick. "Plant microtubules, their associated proteins and the cell cycle." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318090.
Full textBellamy, Matthew Laurence. "A study of non-erythroid isoforms of protein 4.1." Thesis, University of Kent, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298166.
Full textMarks, John. "Cytological and biochemical investigation of actin in the fission yeast Schizosaccharomyces pombe." Thesis, University College London (University of London), 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337930.
Full textWong, Chun-ming. "B-Catenin mutations and expression in hepatocellular carcinoma." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B24729942.
Full textHoyos-Flight, Monica. "Wnt signalling and the regulation of the axonal cytoskeleton." Thesis, Imperial College London, 2005. http://hdl.handle.net/10044/1/11773.
Full textFlear, Andrea Karen. "The cytoskeleton during muscle cell fusion in early myogenesis." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236303.
Full textShuttleworth, Rebecca Jane. "Role of the chondrocyte cytoskeleton in health and disease." Thesis, Cardiff University, 2010. http://orca.cf.ac.uk/54356/.
Full textThodey, Catherine. "Actin cytoskeleton dynamics mediate sugar response in Arabidopsis thaliana." Thesis, University of East Anglia, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.518364.
Full textHawkins, Stephen Francis. "Studies relevant to the cytoskeleton in chronic lymphocytic leukaemia." Thesis, University of Liverpool, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.539727.
Full textKleinJan, Fenneke [Verfasser]. "Viscoelastic and structural properties of the cytoskeleton / Fenneke KleinJan." Ulm : Universität Ulm, 2018. http://d-nb.info/1155827732/34.
Full textTong, Peter. "Interactions between cell membrane dynamics, the cytoskeleton and insulin." Thesis, University of Newcastle Upon Tyne, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242381.
Full textPrice, Leo Sebastian. "Secretion and the actin cytoskeleton in rat mast cells." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307776.
Full textFilippi, Beatrice Maria. "Cellular effects of phosphoinositide derivatives on the actin cytoskeleton." Thesis, Open University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424620.
Full textRocchetti, Alessandra. "Interactions between the plant Golgi apparatus and the cytoskeleton." Thesis, Oxford Brookes University, 2016. https://radar.brookes.ac.uk/radar/items/e035b419-1acc-4031-aadd-2cfc1f9ed3c8/1/.
Full textDickinson, Sarah. "Regulation of the actin cytoskeleton by ephrin-B signalling." Thesis, University College London (University of London), 2006. http://discovery.ucl.ac.uk/1445415/.
Full textFan, Yi. "POLARIZATION OF CYTOSKELETON-REGULATORY PROTEINS DURING ENDOTHELIAL CELL MIGRATION." Case Western Reserve University School of Graduate Studies / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1247148451.
Full textCaution, Kyle J. "Legionella pneumophila and caspases: modulation of the actin cytoskeleton." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449147516.
Full textRodrigues, Joana Nogueira. "Dissecting the role of adducin in the axonal cytoskeleton." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/14771.
Full textThe neuronal cytoskeleton is an interconnected network of filamentous polymers, having in its constitution three major components: actin, microtubules and intermediate filaments. Up to the discovery of axon actin rings, the neuronal actin cytoskeleton has gained relevance. Still, the molecular details of the regulation of the actin cytoskeleton in neurons are largely unknown. Helping in the actin cytoskeleton regulation and maintenance, there is adducin. Adducin is organized in heterotetamers of heterodimers which comprises α/β and α/γ subunits. In the nervous system, the depletion of α subunit results in an almost complete absence of functional adducin. Given this, α-adducin KO mice arose as relevant models to study the role of this protein in actin cytoskeleton. Results from our group showed that α-adducin KO mice develop progressive axon enlargement and degeneration. As defects in axonal transport have been related to axon enlargement, we determined the importance of adducin in the axonal cytoskeleton and, more specifically, in axonal transport. Although no differences were found in the retrograde transport of CTB in the optic nerve, the lack of adducin resulted in a decreased speed of axonal transport of mitochondria and lysosomes. Several neurodegenerative disorders have been associated with axonal transport deficits and, consequently, with alterations in MT-based transport. Although no differences were found in the levels of acetylated and de-tyrosinated tubulin, the levels of tyrosinated tubulin were significantly decreased in α-adducin KO brains, suggesting a less dynamic status of the MT cytoskeleton in the absence of adducin. Besides differential PTMs of tubulin the decreased axonal transport speed may result from the decreased levels of dynein and kinesin in α-adducin KO mice. Lastly, we hypothesized that adducin might be involved in the organization and/or plasticity of the AIS that requires actin dynamics. In α-adducin KO animals, although the AIS forms normally, neurons do not have the ability to relocate it in response to chronic depolarization. Still, the specific role of actin and its associated proteins, like adducin, in this process remains unclear. In sum, with this Thesis we contributed to understand the relevance of the actin in cytoskeleton, more specifically, of the actin-binding protein adducin in neuron biology.
O citoesqueleto neuronal é maioritariamente constituído por três componentes: actina, microtúbulos e filamentos intermédios. Com a descoberta dos anéis de actina presentes no axónio, o citoesqueleto neuronal de actina tem vindo a ganhar bastante relevância. Contudo, os mecanismos moleculares envolvidos na regulação da actina no citoesqueleto continuam por esclarecer. Nesta tese focámo-nos no estudo da importância da aducina, uma proteína de ligação à actina, na regulação do citoesqueleto neuronal. A aducina é uma proteína constituída por heterotetrameros de heterodímeros das subunidades α/β e α/γ, sendo que no sistema nervoso, a depleção da subunidade α resulta numa completa ausência da proteína no seu estado funcional. Assim, murganhos KO para α-aducina demonstraram ser um modelo animal relevante para o estudo do papel desta proteína no citoesqueleto de actina. Resultados do nosso grupo demonstraram que murganhos KO para α-aducina desenvolvem uma degeneração progressiva e um aumento do calibre do axónio. Uma vez que defeitos no transporte axonal têm vindo a ser relacionados com o alargamento axonal, tornou-se importante determinar o papel da aducina no citoesqueleto axonal, mais especificamente no transporte ao longo do axónio. Apesar de não terem sido encontradas diferenças no transporte da toxina da cólera no nervo óptico, a ausência da aducina resultou num decréscimo significativo na velocidade de transporte axonal de mitocôndrias e lisossomas. Diversos distúrbios neurodegenerativos têm sido associados com deficiências no transporte axonal consequentes de alterações na maquinaria de transporte incluindo microtúbulos e proteínas relacionadas. Apesar de não terem sido encontradas diferenças nos níveis de acetilação e de-tirosinação da tubulina em amostras de cérebro α-aducina KO, os níveis de tirosinação da tubulina estão significativamente diminuídos quando comparados com aqueles encontrados em amostras WT, sugerindo uma menor dinâmica dos microtúbulos na ausência de aducina. Além das modificações da tubulina, a diminuição da velocidade de transporte axonal poderá resultar também do decréscimo dos níveis de ambos os motores moleculares dineina e cinesina nos murganhos α-aducina KO. Por fim, sugere-se que a aducina poderá também estar envolvida na organização e/ou plasticidade do segmento inicial do axónio dada a sua ligação ao citoesqueleto de actina, importante para a sua função e organização. Nos murganhos KO para α-aducina, foi verificado que apesar da formação do segmento inicial ser normal, as células não têm a capacidade para o relocalizar após uma depolarização crónica. Porém, o papel específico da actina e das suas proteínas associadas, tal como a aducina, neste processo deverá ser investigado com maior detalhe. Sumariamente, com esta tese, foi possível contribuir para uma melhor compreensão da relevância da actina, mais especificamente, da proteína de ligação à actina aducina, na biologia de um neurónio.
Pereira, José Carlos Ribeiro Ferreira. "Cytoskeleton regulation in bladder cancer cells after photodynamic treatment." Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/21089.
Full textA terapia fotodinâmica (PDT) é uma modalidade promissora para o tratamento do cancro. Esta terapia baseia-se na interação entre um composto químico (fotossensibilizador, PS), luz com um determinado comprimento de onda e oxigénio molecular para originar a produção de espécies reativas de oxigénio (ROS). Devido à sua elevada reatividade, estas espécies tóxicas podem causar danos severos conduzindo à morte celular. Atualmente, os PS disponíveis na clínica para o tratamento de tumores apresentam baixa seletividade para as células tumorais. Estudos anteriores do nosso grupo descreveram uma porfirina conjugada com unidades dendríticas de galactose (PorGal8) como um novo PS solúvel em solução aquosa, capaz de gerar ROS após fotoativação e com reconhecimento por parte de proteínas (galectina-1) que se encontram sobreexpressas nas células do cancro da bexiga. Vários estudos têm descrito alterações no citoesqueleto em resposta ao tratamento fotodinâmico. No entanto, a contribuição da desorganização do citoesqueleto na morte celular induzida por PDT encontra-se pouco esclarecida. Neste trabalho, avaliámos de que forma alterações nos constituintes do citoesqueleto – filamentos de actina, filamentos intermédios e microtúbulos – estão relacionadas com morte celular induzida por PDT com PorGal8. O uptake de PorGal8 em duas linhas celulares do cancro da bexiga derivadas de carcinoma de células transicionais (UM-UC-3 e HT-1376), foi dependente da concentração. O uptake celular de PorGal8 foi superior nas células UM-UC-3, que exibem níveis superiores da proteína galectina-1, comparativamente com as células HT-1376. PorGal8 mostrou não ser tóxico no escuro. A fotoativação da PorGal8 resultou numa fototoxicidade significativamente superior nas células UM-UC-3 relativamente às células HT-1376. A PorGal8 não induziu alterações significativas nos níveis de proteína α-tubulina nas células UM-UC-3. No entanto, observou-se uma redução significativa nos níveis de α-tubulina nas células HT-1376 vinte e quatro horas após tratamento com irradiação. Apesar de se ter observado uma recuperação na organização dos microtúbulos em algumas células, a intensidade da fluorescência diminuiu consideravelmente na maior parte das células HT-1376. Uma redução significativa nos níveis de proteína dos filamentos intermédios (vimentina) foi observada em ambas as linhas celulares vinte e quatro horas após irradiação. Trinta minutos após a irradiação, as células UM-UC-3 e HT-1376 apresentaram uma clara retração nos filamentos de actina com perda de fibras de stress. Ao contrário das células UM-UC-3 em que não se verificaram sinais de recuperação, em algumas células HT-1376 verificou-se uma certa reorganização dos filamentos de actina, com curtas fibras de stress, longas extensões, grandes filopodia, o que parece sugerir uma possível recuperação das células HT-1376. A RhoA, uma proteína da família de pequenas proteínas GTPases, descrita como estando relacionada com a expressão da galectina-1, foi adicionalmente avaliada. Resultados preliminares indicaram que a PorGal8 induziu uma tendência para aumentar os níveis de RhoA nas células HT-1376 vinte e quatro horas após tratamento com irradiação. Concluindo, os nossos resultados contribuem para o esclarecimento dos mecanismos subjacentes dos efeitos fototóxicos da PorGal8. Uma melhor compreensão dos intervenientes e das alterações induzidas imediatamente após PDT nas estruturas do citoesqueleto em cancros resistentes à terapia, poderão contribuir para o desenvolvimento de novos agentes terapêuticos adjuvantes à PDT.
Photodynamic therapy (PDT) is a promising modality for the treatment of cancer that involves light of an appropriate wavelength and a photosensitizing drug (photosensitizer, PS), used in conjunction with molecular oxygen, leading to the production of reactive oxygen species (ROS). In a biological environment, these toxic species can interact with the cellular constituents eliciting cell death. Currently, the PS available show poor tumor specificity. Previous work from our research group reported a porphyrin conjugated with dendritic units of galactose (PorGal8) as a new water soluble PS, able to generate ROS after photoactivation and exhibiting increased selectivity to bladder cancer cells overexpressing galectin-1. Several studies reported cytoskeleton alterations derived from photodynamic treatments. However, the role of cytoskeleton disorganization in cell death induced by PDT remains unclear. In this work we evaluated whether changes in the cytoskeletal constituents - actin filaments, intermediate filaments and microtubules - are correlated with cell death triggered by PDT with PorGal8. The uptake of PorGal8 in two bladder cancer lines derived from transitional cell carcinoma (UM-UC-3 and HT-1376 cells), was concentration dependent. Cellular uptake of PorGal8 was higher in UM-UC-3 cells that express higher levels of galectin-1 protein than HT-1376 cells. PorGal8 was nontoxic in dark. Photoactivation of PorGal8 resulted in a significantly higher phototoxicity in UM-UC-3 cells than HT-1376 cells. PorGal8 did not change the α-tubulin protein levels in UM-UC-3 cells but reduced α-tubulin twenty-four hours after photodynamic activation in HT-1376 cells. Although a few cells showed a recovery in microtubules organization, the fluorescence intensity decreased noticeably in most of the HT-1376 cells. A significant decrease in intermediate filaments (vimentin) protein levels was exhibited in both cell lines twenty-hours after irradiation. Thirty minutes post-irradiation, UM-UC-3 and HT-1376 cells showed a clear retraction of actin filaments with loss of stress fibers. Although no recovery was observed in UM-UC-3 cells, some cells present some reorganization in actin filaments, presenting short stress fibers, long extensions, like large filopodia, suggesting a possible recovery in HT-1376 cells. A small GTPases family protein, RhoA, referred to be involved with galectin-1 expression, was also evaluated, with preliminary results indicating a tendency towards an increase in HT-1376 cells twenty-four hours after therapy. Overall, our results give new insights into the mechanisms underlying the phototoxic effects of PorGal8. Better understanding the intrinsic web of events and alterations on cytoskeleton structures induced immediately after photodynamic treatment in resistant cancers may contribute to envisage new potential therapeutic adjuvants for PDT.
Martin, Stuart S. "Phosphoinositide-dependent regulation of the actin cytoskeleton by insulin /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9904827.
Full textZimmerman, Matthew J. "Regulation of the cytoskeleton in human microvascular endothelial cells /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9956453.
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