Dissertations / Theses on the topic 'Cytomegaloviruses'
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Walker, Douglas Gordon. "Characterization of immediate-early and early proteins of murine cytomegalovirus synthesized in permissive and nonpermissive cells." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/25985.
Full textMedicine, Faculty of
Pathology and Laboratory Medicine, Department of
Graduate
Vellani, Nina N. "Analyses of immediate early and early transcripts and major early region, E10, of murine cytomegalovirus." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/32374.
Full textMedicine, Faculty of
Pathology and Laboratory Medicine, Department of
Graduate
Kansara, Viral Mitra Ashim K. "Ocular delivery of peptide ganciclovir prodrugs following subconjunctival injection evaluation of episcleral drug delivery approach /." Diss., UMK access, 2007.
Find full text"A dissertation in pharmaceutical sciences and pharmacology." Advisor: Ashim K. Mitra. Typescript. Vita. Title from "catalog record" of the print edition Description based on contents viewed May 23, 2008. Includes bibliographical references (leaves 210-225). Online version of the print edition.
Chaudry, Tanya N. "Characterisation of PP71 homologues encoded by mammalian cytomegaloviruses." Thesis, Connect to e-thesis, 2008. http://theses.gla.ac.uk/377/.
Full textPh.D. thesis submitted to the Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, 2008. Includes bibliographical references. Print version also available.
Jun, Min Medical Sciences Faculty of Medicine UNSW. "Analysis of human cytomegalovirus susceptibility to novel antiviral agents." Publisher:University of New South Wales. Medical Sciences, 2008. http://handle.unsw.edu.au/1959.4/41443.
Full textAndrews, Daniel Mark. "Effects of murine cytomegalovirus infection on dendritic cell functionality and natural killer cell responses." University of Western Australia. Centre for Ophthalmology and Visual Science, 2004. http://theses.library.uwa.edu.au/adt-WU2005.0003.
Full textSumaria, Nital. "The relevance of specific molecular and cellular effectors during murine cytomegalovirus infection." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0116.
Full textChen, Jianliang, and 陈健良. "The inhibitory effects of human cytomegalovirus on megakaryopoiesis : megekaryocytic cells and bone marrow derived mesenchymal stormal cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193520.
Full textpublished_or_final_version
Paediatrics and Adolescent Medicine
Doctoral
Doctor of Philosophy
Bartlett, Emmalene J. "Efficacy and immunological mechanisms of type 1 interferon gene therapy in murine cytomegalovirus." Thesis, Bartlett, Emmalene J. (2002) Efficacy and immunological mechanisms of type 1 interferon gene therapy in murine cytomegalovirus. PhD thesis, Murdoch University, 2002. https://researchrepository.murdoch.edu.au/id/eprint/214/.
Full textBartlett, Emmalene J. "Efficacy and immunological mechanisms of type 1 interferon gene therapy in murine cytomegalovirus." Bartlett, Emmalene J. (2002) Efficacy and immunological mechanisms of type 1 interferon gene therapy in murine cytomegalovirus. PhD thesis, Murdoch University, 2002. http://researchrepository.murdoch.edu.au/214/.
Full textKhong, Andrea. "Effect of murine cytomegalovirus infection on haematopoiesis and myeloid cell differentiation and function." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0260.
Full textStone, Matthew. "Evaluation of the therapeutic efficacy of bulk-cultured cytotoxic T lymphocytes in primary murine cytomegalovirus infection." Virtual Press, 1991. http://liblink.bsu.edu/uhtbin/catkey/774736.
Full textCenter for Medical Education
Towler, James Charles. "Transcriptome activity of human cytomegalovirus (strain Merlin) in fibroblasts, epithelial cells and astrocytes." Thesis, Connect to e-thesis record to view abstract. Move to record for print version, 2007. http://theses.gla.ac.uk/42/.
Full textPh.D. thesis submitted to the Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, 2007. Includes bibliographical references. Print version also available.
Pechenick, Jowers Tali. "Investigation of murine cytomegalovirus modulation of TLR/IL-1β signalling pathways." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/6478.
Full textHuygens, Ariane. "Fetal T cell response to human congenital cytomegalovirus infection." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209450.
Full textLes lymphocytes T CD4+ Th1 et les lymphocytes T CD8+ cytotoxiques jouent un rôle crucial dans le contrôle des pathogènes intracellulaires dont le HCMV fait partie. La littérature montre une capacité limitée des enfants congénitalement infectés par le HCMV à développer des réponses T CD4+ spécifiques du HCMV. En contraste, des réponses de lymphocytes T CD8+ spécifiques du HCMV ont été rapportées chez des enfants infectés in utero, mais ces réponses n’ont pas été comparées en détails à celles de l’adulte. De plus, notre connaissance des réponses T spécifiques du HCMV durant l’infection primaire par ce virus est limitée. Des études antérieures ont rapporté un défaut de prolifération et de production d’IL-2 des lymphocytes T spécifiques du HCMV chez des adultes avec durant la phase primaire de l’infection, mais les mécanismes restent non-élucidés.
Nous avons caractérisé les réponses de lymphocytes T CD4+ et CD8+ spécifiques du HCMV provenant du sang de cordon de nouveau-nés congénitalement infectés par le HCMV, et nous avons comparé ces réponses à celles de leurs mamans diagnostiquées avec une infection primaire par le HCMV durant la grossesse. En plus, nous avons comparé les réponses T CD4+ et CD8+ de ces mamans à celles d’adultes infectés chroniquement par le virus. Chez les nouveau-nés, nous avons démontré que des lymphocytes T CD4+ de sang de cordon exprimant un phénotype de différentiation spécifique du HCMV (CD27-CD28-) ainsi qu’un phénotype Th1 similaire à celui des cellules maternelles étaient induits in utero lors de l’infection congénitale par le HCMV. De plus, la détection d’expansions oligoclonales suggérait fortement une expansion antigène-spécifique de ces cellules. Cependant, les T CD4+ de nouveau-nés présentaient une capacité fortement réduite à produire des cytokines anti-virales (IFN-γ, TNF-α et MIP-1β) en réponse à une stimulation ex vivo avec les antigènes du HCMV, par rapport aux cellules maternelles. Les lymphocytes T (CD27-CD28-) CD4+ de nouveau-nés produisaient également des niveaux plus bas de cytokines antivirales en réponse à des stimulations polyclonales avec l’anti-CD3 et la PMA/ionomycine, suggérant des altérations en amont et en aval de la voie de signalisation du TCR. Nos résultats suggèrent que ces altérations pourraient impliquer la diminution de l’expression de molécules impliquées dans cette voie de signalisation. De la même manière, nous
avons montré que chez le nouveau-né, la fonction des T CD8+ spécifiques du HCMV était altérée par rapport à celle de l’adulte. Nous avons observé des proportions similaires de T CD8+ (CD27-CD28-) chez les nouveau-nés et les adultes. De plus, l’analyse du répertoire du TCR Vβ de ces cellules par séquençage haut-débit a révélé une capacité similaire à générer un répertoire T diversifié dans les deux groupes. Comme rapporté précédemment, nous avons détecté des fréquences similaires de lymphocytes T CD8+ spécifiques pour l’antigène immunodominant pp65. Cependant, lorsque les stimulations ont été étendues à d’autres antigènes du HCMV, nous avons observé que le répertoire antigénique reconnu par ces cellules était significativement réduit chez les nouveau-nés, en association avec une diminution de la polyfonctionalité et de la production de cytokines par cellule.
Nous avons également montré que, dans une moindre mesure, la fonction des lymphocytes T spécifiques du HCMV était diminuée durant l’infection primaire chez l’adulte. Comme reporté précédemment, les T CD4+ spécifiques du HCMV proliféraient moins et produisaient moins d’IL-2 par rapport à des individus dans la phase chronique de l’infection. Ce défaut de production d’IL-2 affectait à la fois les populations de cellules CD28+ et CD28-, montrant que l’accumulation de lymphocytes T CD4+ ayant perdu l’expression de la molécule CD28 (un signal de co-stimulation important pour la production d’IL-2) est seulement un des facteurs contribuant à la diminution de la production d’IL-2 par les cellules spécifiques du HCMV. En accord avec cette observation, nous avons montré une diminution de la production par cellule d’IFN-γ et de TNF-α touchant également à la fois les populations de T CD4+ CD28+ et CD28- durant la phase primaire de l’infection, un défaut associé avec une avidité fonctionnelle diminuée de ces cellules. De la même manière, la polyfonctionalité et la production de cytokines par cellule des lymphocytes T CD8+ spécifiques du HCMV étaient également diminuées chez les adultes durant la phase d’infection primaire.
En résumé, nos résultats montrent que la fonction des lymphocytes T spécifiques du HCMV de nouveau-nés et d’adultes est altérée durant l’infection primaire par rapport à des individus infectés chroniquement par le virus. Nous montrons que cette régulation fonctionnelle ressemble à l’exhaustion fonctionnelle des lymphocytes T observée durant les infections virales chroniques associées à des charges virales élevées. L’infection primaire par le HCMV est caractérisée par une réplication virale intense qui dure pendant plusieurs mois suivant l’infection. Nous émettons l’hypothèse que les hauts taux de réplication virale observés durant l’infection congénitale et chez l’adulte durant l’infection primaire par le HCMV pourraient interférer avec certaines fonctions des lymphocytes T./Neonates and young infants have a higher susceptibility to infections compared to older infants or adults. This feature is in part attributed to the immaturity of their immune system associated with a limited capacity to mount cellular-mediated immune responses. Congenital human cytomegalovirus (HCMV) infection is the most common cause of congenital infection worldwide and a major cause of hearing loss and mental retardation. In Belgium, antenatal screening of pregnant women for primary HCMV infection offers an opportunity to study neonatal immune responses to the virus and to compare them to those of their mother.
T lymphocytes are major players of the immune system. In particular, Th1 CD4+ T cells and CD8+ cytotoxic T cells play a crucial role in the control of intracellular pathogens, including HCMV infection. Previous literature has reported a limited capacity of infants born with congenital HCMV infection to mount HCMV-specific CD4+ T cell responses. In contrast, fetal antigen-specific CD8+ T cell responses have been reported following in utero HCMV infection, but these responses have not been compared in detail to those of adults with primary infection. In addition, our knowledge regarding adult HCMV-specific T cell responses during primary HCMV infection is limited. Previous studies have reported defective T cell proliferation and IL-2 production in adults with primary HCMV infection, showing that some of the T cell functions are altered during primary infection.
In this study, we have characterized neonatal HCMV-specific CD4+ and CD8+ T cell responses from the cord blood of newborns with congenital HCMV infection, and we have compared these responses to that of their mothers diagnosed with primary HCMV infection during pregnancy. Also, we compared CD4+ and CD8+ T cell responses of adults with primary HCMV infection to that of adults with chronic infection.
In newborns, it was not known if the defective CD4+ T cell responses could be attributed to the absence of HCMV-specific cells or to the induction of dysfunctional cells. We demonstrate that neonatal CD4+ T cells with a differentiation phenotype typical of HCMV infection (CD27-CD28-) and expressing a Th1 phenotype similar to that of maternal cells can differentiate in utero following HCMV infection. In addition, the detection of oligoclonal expansions by spectratyping and flow cytometry analyses strongly suggests antigen-specific responses. However, neonatal CD4+ T cells were markedly less able to produce antiviral cytokines (IFN-γ, TNF-α and MIP-1β) following ex vivo stimulation with HCMV antigens, compared to maternal cells. Also, neonatal CD27-CD28- CD4+ T cells produce lower levels of antiviral cytokines in response to polyclonal stimulations with anti-CD3 and PMA/ionomycin, suggesting alterations up-stream and down-stream of the TCR signaling pathway. Our results suggest that these alterations could involve the down-regulation of the expression of molecules that are part of the TCR signaling pathway. Similarly, we show that the function of
neonatal HCMV-specific CD8+ T cells is impaired compared to adults. Similar proportions of (CD27-CD28-) CD8+ T cells, typical of HCMV infection, were detected in newborns and adults. Analysis of the TCR Vβ repertoire of neonatal and maternal (CD27-CD28-) CD8+ T cells by high-throughput sequencing revealed a similar capacity to generate a diverse clonal repertoire. As previously reported, we detected similar frequencies of HCMV-specific CD8+ T cells specific for the immunodominant viral antigen pp65. However, when extending ex vivo stimulations to other HCMV antigens, we observed that the antigenic repertoire recognized by these cells was significantly reduced in newborns. In addition, neonatal CD8+ T cells had a reduced polyfunctionality and per cell cytokine production.
To a lower extent, the function of adult HCMV-specific T cells was also impaired during primary infection. As previously reported, maternal HCMV-specific CD4+ T cells were markedly less able to produce IL-2 and to proliferate compared to individuals in the chronic stage of the disease. Both CD28+ and CD28- T cell subsets produced decreased levels of IL-2. This observation shows that the accumulation of HCMV-specific CD4+ T cells having lost the expression of the CD28 molecule (an important co-stimulatory signal for IL-2 production) during primary infection is only one of the factors contributing to the decreased IL-2 production. Accordingly, both CD28+ and CD28- CD4+ T cell subsets had a decreased per cell production of IFN-γ and TNF-α during primary HCMV infection. This defect was associated with a lower functional avidity of these cells. Similarly, the polyfunctionality and per cell cytokine production of adult HCMV-specific CD8+ T cells was also impaired compared to adults with chronic infection.
Altogether, our results show that adult and neonatal HCMV-specific T cell responses are impaired during primary infection, compared to individuals with chronic infection. We show that this functional regulation resembles that of functional T cell exhaustion observed during chronic viral infections that are associated with high levels of viral replication. Primary HCMV infection is characterized by an intense viral replication lasting for several months post-infection. We hypothesize that the high levels of viral replication observed during congenital and adult primary HCMV infection could interfere with some of the T cell functions.
Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Albuquerque, Dulcinéia Martins de. "Aspectos moleculares do citomegalovirus humano durante infecção ativa em pacientes submetidos ao transplante de medula ossea." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311935.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: O Citomegalovírus Humano (HCMV) continua sendo uma causa significante de morbidade em pacientes imunocomprometidos, especialmente em transplantados de medula óssea, e pode manifestar diversas complicações que incluem hepatite, doença gastrointestinal e pneumonia intersticial ou a denominada "Síndrome Viral por HCMV"caracterizada por febre, leucopenia e trombocitopenia. O HCMV pode também ter um efeito imuno-modulador, fazendo da infecção por esse vírus um fator de risco importante para o desenvolvimento de rejeição ao enxerto aguda e crônica e para co-infecção com outras herpesviroses. A detecção do genoma do HCMV pela PCR (Reação em Cadeia da Polimerase) é específica e sensível, e pode ser usada como uma poderosa ferramenta para o diagnóstico precoce da infecção causada por este vírus. Variações em regiões funcionalmente relevantes do genoma do HCMV têm sido utilizadas como marcadores genéticos em diversos estudos clínicos para diferenciar as linhagens do vírus e associá-las com a patogênese viral e com as manifestações clínicas no paciente. A glicoproteína B (gB) é a maior glicoproteína do envelope do HCMV e tem sido relacionada à entrada na célula hospedeira, transmissão célula-a-célula, e conseqüentemente à fusão das células infectadas. A amplificação do gene gB pela PCR combinada com análise de restrição por RFLP em regiões polimórficas deste gene são eficientes para a identificação dos genótipos do HCMV, tornando possível a distinção de pelo menos 4 padrões eletroforéticos. Por outro lado, a determinação da carga viral em pacientes imunologicamente afetados tem sido associada como marcador ou preditor do desenvolvimento de doença por HCMV órgão-específica. Sendo assim, a determinação da carga viral, especificamente nestes pacientes, é fundamental para a supervisão da terapia antiviral. Além disso, os valores da carga viral estão relacionados aos níveis de imunossupressão, à patogênese do HCMV e ao grupo de pacientes e/ou ao tipo de transplante e podem indicar o início da administração da terapia antiviral.O método de real-time PCR (RT-PCR) foi aplicado para a quantificação do genoma do HCMV em amostras clínicas e a detecção e posterior quantificação do DNA do HCMV em amostras de soro por esta técnica é capaz de distinguir entre pacientes com infecções sintomáticas daqueles com infecções inativas ou latente.Avanços têm sido feitos na prevenção da doença por HCMV após o transplante de medula óssea, inclusive a administração profilática, por períodos prolongados, de antivirais como o Acyclovir e o Ganciclovir e como conseqüência, pode originar linhagens resistentes relacionadas principalmente a dois genes virais: a fosfotransferase viral (UL97) e a DNA polimerase viral (UL54). Sabendo-se da importância da identificação das linhagens do HCMV em pacientes transplantados de medula óssea e da possível relação com a infecção e apresentação clínica; da relevância em determinar a carga viral como preditor de doença; e finalmente, da detecção de linhagens resistentes aos agentes antivirais disponíveis, este estudo avaliou, prospectivamente, pacientes transplantados de medula óssea em seguimento no Hemocentro/UNICAMP. Além disso, teve como principais objetivos: determinar a prevalência dos genótipos do HCMV e avaliar uma possível associação com a apresentação clínica nesses pacientes; determinar a carga viral para o monitoramento da terapia antiviral; e identificar e correlacionar mutações que conferem resistência ao Ganciclovir com carga viral e apresentação clínica. Foram incluídas na casuística, 169 amostras de DNA de sangue periférico e 187 amostras de DNA de soro de 22 pacientes transplantados de medula óssea. Dentre as 47 amostras de DNA de sangue periférico HCMV positivas, 42 foram genotipadas e observamos a prevalência do genótipo gB1 (47%) como descrito em literatura, e embora sem comprovação estatística, notamos a tendência deste genótipo com melhor prognóstico. Aplicamos a RT-PCR em 96 amostras de DNA de soro de 12 pacientes transplantados de medula óssea seguidos no Ambulatório de Hematologia, e observamos que o método é adequado para a avaliação da carga viral neste grupo de pacientes. No entanto, é necessário estabelecer um valor de corte a fim de se utilizar esta metodologia para obtenção de um valor que seja preditivo de doença e para o monitoramento do tratamento dos pacientes. Este método mostrou-se mais preciso que a ?nested?-PCR no mesmo tipo de amostra. Além disso, identificamos 8 novas mutações no gene UL97, uma delas pode estar relacionada à resistência viral ao Ganciclovir. Dentre os polimorfismos identificados, 3 parecem estar relacionados ao genótipo gB1 e possivelmente podem ser utilizadas como marcadores genéticos para a genotipagem do HCMV. Para o gene UL54 foram identificadas 5 novas mutações na região IV do gene e que geralmente é relacionada à resistência ao Ganciclovir. Nós concluímos que a determinação da carga viral é importante, mas não é o único modo de avaliar a eficiência do tratamento antiviral. Dessa forma, a avaliação de outros parâmetros moleculares, como a genotipagem e mutações relacionadas à resistência aos antivirais, são informações complementares e devem ser consideradas para o monitoramento da evolução clínica em pacientes transplantados de medula óssea
Abstract: Human Cytomegalovirus (HCMV) remains a significant cause of morbidity in immunocompromised patients, especially in bone marrow transplant recipients. It may manifest severe complications including hepatitis, gastrointestinal disease, and interstitial pneumonitis or as so-called ?HCMV viral syndrome? with fever, leukopenia, and thrombocytopenia. The HCMV may also has an immunomodulatory effect, potentially making HCMV infection an important risk factor for the development of an acute and chronic allograft rejection and for coinfection with other herpesviruses. The detection of the HCMV genome by PCR (Polymerase Chain Reaction) is specific and sensitive. Besides this, it can be used as a powerful tool for the early diagnoses of the infection caused by this virus. Variations in functionally relevant areas of the HCMV genome have been used as genetic markers in numerous clinical studies to differentiate the HCMV strains and to associate them with the viral pathogenesis further with the patients? clinical manifestations. The glycoprotein B (gB) is the major glycoprotein of HCMV?s envelope and it has been implicated in host cell entry, cell-to-cell virus transmission, consequently in the fusion of infected cells. The gB amplification by PCR combined with the restriction analysis by RFLP in polymorphic areas are effective for the identification of the HCMV genotypes, becoming possible the distinction of at least 4 electrophoretic patterns. On the other hand, the determination of the viral load in the immunologically affected patients has been associated as marker or predictor for the development of the organ specific disease by the HCMV. Hence, the determination of the viral load in these specific patients is fundamental for the management of the antiviral therapy. In addition, the viral load values are related to levels of the immune-suppression, the pathogenesis of the HCMV and the group of patients and/or the type of transplant. Furthermore, the viral load values can indicate the beginning of the antiviral therapy administration. A real-time PCR (RT-PCR) assay was applied for quantifying the HCMV genome load in clinical samples and the detection and quantification of HCMV DNA in blood serum through RT-PCR are able to distinguish patients with symptomatic infections among those with latent or inactive infections. Advances have been made in the prevention of HCMV disease after bone marrow transplantation, including prophylactic administration of antivirals such as Acyclovir and Ganciclovir. The HCMV prophylaxis with antiviral in this patients? group is administered for prolonged periods of therapy, consequently it can originate resistant viruses related mainly to two genes: the viral phosphotransferase (UL97) and the viral DNA polymerase (UL54). Ahead the importance of the identification of HCMV strains in bone marrow transplant patients, the HCMV strains performance in the patients? infection and clinical presentation, the relevance of determinating the viral load as a disease predictor, and finally, the detection of the resistant strains to the available antivirals, this study prospectively evaluated bone marrow transplant recipients followed at Hemocentro/UNICAMP. Moreover, it had as main goals: to determine the prevalence of the HCMV gB genotypes, to evaluate a possible gB genotype association with the patients? clinical presentation; to determinate the viral load for monitoring the antiviral therapy, and to correlate Ganciclovir resistant mutations in UL97 and UL54 gene with the viral load and patients? clinical presentation. From 22 bone marrow transplant recipients, DNA samples of peripheral blood (169) and DNA samples of blood serum (187) were included in this casuistic. Among 47 HCMV positive samples, 42 were genotyped. We observed the prevalence of gB1 genotype (47%), as described in the specific literature, however without statistical analysis, the raw data exhibited that gB1 genotype can be related to patients? better prognostics. From 12 followed bone marrow transplant recipients, we applied the RT-PCR in 96 DNA blood serum samples and we observed that the method was accurate for the viral load evaluation in this patients? group. However, it is necessary to establish a crucial cutoff to consider whether a specific value of viral load is a predictive value to cause HCMV disease and to monitor the patients? treatment. This method was more precise than the nested-PCR for blood serum samples. Additionally, we identified 8 new mutations in UL97 gene, one of them can be related to Ganciclovir HCMV resistance. Among all of identified polymorphisms, 3 of them can be related to gB1 genotype and may be used as genetic marker to HCMV genotyping. In the region IV of the UL54 gene, 5 new mutations were identified, and can possibly be related to Ganciclovir HCMV resistance. We concluded that the determination of the patients? viral load is crucial, even so it is not the only way to evaluate the antiviral treatment efficacy. Then, the evaluation of other molecular parameters as genotyping and mutations related to the HCMV antiviral resistance, are complementary information and must be considered to monitor the clinical evolution of bone marrow transplant recipients
Doutorado
Ciencias Basicas
Doutor em Clínica Médica
Antoine, Pierre. "Etude de la réponse des lymphocytes T CD4+ au cours de l'infection primaire par le cytomégalovirus." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209148.
Full textAprès l’infection primaire, le virus persiste tout au long de la vie à l’état latent mais peut se réactiver de manière intermittente. Ceci est associé à l’expansion de lymphocytes T CD4+ fortement différenciés ayant des fonctions auxiliaires et cytolytiques. L’infection primaire est, par contre, caractérisée par une réplication virale intense qui dure plusieurs mois. Il a été montré que l’exposition prolongée à des concentrations élevées d’antigènes entraine une perte progressive de fonction par les lymphocytes T appelée épuisement et caractérisée par l’expression de récepteurs inhibiteurs. L’impact de la réplication virale intense observée au cours de l’infection primaire par le CMV sur la fonction des lymphocytes T CD4+ n’est pas bien connu.
La fonctionnalité des lymphocytes T CD4+ a été explorée chez l’humain et le singe rhésus au cours de l’infection primaire et comparée à celle de sujets porteurs chroniques du virus.
Les résultats montrent que l’infection primaire par le CMV est associée à la détection de lymphocytes T CD4+ circulants ayant une faible capacité de prolifération et de production de cytokines et d’IL-2 en particulier.
L’impact de la différenciation sur la fonction des lymphocytes a été exploré en détail chez l’humain. Il a été observé qu’un degré de différenciation plus élevé des lymphocytes T CD4+ spécifiques du CMV joue un rôle dans la production réduite d’IL-2. Toutefois, la fraction moins différenciée (exprimant la molécule CD28) présente également une sécrétion d’IL-2 moindre au cours de l’infection primaire. Ceci fait partie d’une diminution globale de la production de cytokines au cours de l’infection primaire qui affecte également la sécrétion d’IFNγ et TNFα, entraine une polyfonctionnalité réduite et est indépendante de la différenciation. L’épuisement des lymphocytes T CD4+ spécifiques du CMV contribue à leur fonctionnalité moindre comme l’indique l’expression accrue du récepteur inhibiteur PD-1 et l’augmentation des réponses prolifératives en présence d’anticorps bloquant PD-1.
Le lien entre excrétion virale et fonction lymphocytaire a été étudié chez le macaque rhésus. L’infection par le CMV est observée chez les singes juvéniles et adultes mais pas chez les nourrissons. L’excrétion urinaire et salivaire est significativement plus fréquente et intense chez les singes juvéniles par rapport aux adultes. Comme chez l’humain au cours de l’infection primaire, les lymphocytes T CD4+ spécifiques du virus sont moins
polyfonctionnels et prolifèrent moins efficacement chez les singes juvéniles par rapport aux singes adultes. Ceci est associé à l’expression accrue du récepteur inhibiteur PD-1 chez les singes juvéniles. La réponse proliférative des lymphocytes T CD4+ est accrue en présence d’anticorps bloquant PD-1 ou d’IL-2 exogène. Enfin, une association inverse entre fonction lymphocytaire et excrétion urinaire a été mise en évidence chez les macaques adultes.
Ces résultats indiquent que l’infection par le CMV présente des caractéristiques semblables chez l’humain et le singe rhésus. L’infection primaire est associée à la détection de lymphocytes T CD4+ ayant une fonctionnalité moindre qu’au cours de l’infection chronique. L’expression du récepteur inhibiteur PD-1 typique des cellules épuisées est l’un des mécanismes impliqués et pourrait être la cible de stratégies immunomodulatrices visant à améliorer les fonctions lymphocytaires et le contrôle de la réplication virale. Les résultats présentés indiquent que l’infection naturelle chez le singe rhésus constitue un modèle potentiellement utile à l’étude de la réponse immune au CMV humain et à l’évaluation de stratégies immunomodulatrices.
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Cytomegalovirus infection is mostly asymptomatic in immunocompetent hosts but leads to severe morbidity and mortality in immunocompromised subjects and foetuses.
After primary infection, CMV establishes lifelong persistence but can reactivate intermittently. This is associated with the expansion of highly differentiated CD4+ T lymphocytes exhibiting helper functions and cytolytic activity.
Primary infection is characterised by an intense viral replication lasting several months. It has been shown that prolonged exposure to elevated antigen concentrations induces a progressive loss of function by T lymphocytes called exhaustion. This state of functional impairment is associated to the expression of inhibitory receptors. The consequence of the intense viral replication seen in primary CMV infection on CD4+ T cell function is unknown.
CD4+ T cell function has been studied in human and rhesus macaque during primary CMV infection. Chronic CMV carriers have been used as controls.
The results show that primary CMV infection is associated to the detection of circulating CD4+ T lymphocytes exhibiting weak proliferative capacities and reduced cytokine production affecting IL-2 in particular.
The impact of differentiation on lymphocyte function has been explored in detail in human. An increased proportion of terminally differentiated CD4+ T cells (CD28-) is observed during primary infection. These lymphocytes are unable to secrete IL-2 in response to CMV antigens. Interestingly, CD28+ CMV-specific CD4+ T cells also exhibit reduced IL-2 production during primary infection. This is part of a global reduction of cytokine production affecting IFNγ and TNFα as well. The impaired cytokine production is associated to reduced polyfunctionality and is independent of differentiation. Exhaustion of CMV-specific CD4+ T lymphocytes contributes to the reduced functionality as shown by an increased expression of the inhibitory receptor PD-1 and improved proliferative responses in the presence of PD-1 blocking antibodies.
The relationship between viral replication and lymphocyte function has been explored in rhesus macaques. CMV infection is observed in juvenile and adult monkeys but not in newborns. Excretion in urine and saliva is significantly more frequent and intense in juvenile monkeys than adults. As in primary infection in human, CMV-specific CD4+ T lymphocytes are less polyfunctional and have lower proliferative capacities in juveniles as compared to adults. This is associated with an increased expression of PD-1 in juvenile monkeys. CD4+ T cell proliferative responses are increased when PD-1 blocking antibodies or exogenous IL-2 are added to the culture medium. Finally, an inverse association between lymphocyte function and urinary excretion has been observed in adult macaques.
These results indicate that CMV infection shares common features in human and rhesus macaque. Primary infection is associated to the detection of CD4+ T lymphocyte displaying lower functional capacities as compared to chronic infection. Exhaustion contributes to the functional impairment and the inhibitory receptor PD-1 could be targeted by immunomodulatory strategies aiming at improving lymphocyte functions and controlling viral replication. Natural CMV infection in rhesus macaque might be useful as a model to evaluate the efficacy and safety of immunomodulatory approaches.
Doctorat en Sciences médicales
info:eu-repo/semantics/nonPublished
Arendse, Germaine Veronique. "The relationship between Cytomegalovirusspecific cellular immune response and CD4+ T cell count in HIV positive individuals in a South African setting." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6860.
Full textENGLISH ABSTRACT: Introduction: Reactivation of human cytomegalovirus (HCMV) infection in individuals infected with human immunodeficiency virus (HIV) may lead to life-threatening end-organ diseases (EOD). The EOD becomes clinically apparent when a critical number of cells in the affected organs become damaged as a consequence of HCMV-infection. Treatment of the HCMV-associated disease at this point may not be effective. Therefore, early detection of HCMV reactivation may be useful to guide pre-emptive therapy. Aim: The aim of this study was to determine whether there is a point at which the HCMV-specific cellular immune response breaks down, as determined by the interferon-gamma (IFN-γ) enzyme-linked immunospot (ELISPOT) assay, and HCMV reactivation occurs in HIV-positive, antiretroviral therapy (ART)-naïve individuals in a South African setting. This was done in relation to the CD4+ T cell count and the HCMV viral load as determined by real-time polymerase chain reaction (qPCR). Materials and methods: Fifty-two (52) HIV-infected, ART-naïve subjects were recruited from primary healthcare centres that they attended for the management of their HIV infection. Heparinised blood samples were collected to quantify the HCMV-specific cellular immune response using the IFN-γ-ELISPOT assay and to determine the HCMV IgG serostatus. Ethylenediaminetetraacetic acid (EDTA) blood samples were collected for the determination of the CD4+ T cell counts and the HCMV viral loads. Results: All 52 subjects recruited were confirmed to be HIV-HCMV co-infected based on their HCMV IgG serostatus. The results of 34 subjects with completed data sets were analysed. The CD4+ T cell counts of these subjects ranged from 10 to 784 cells/μl. Twenty-two (22) (65%) subjects had positive HCMV IFN-γ-ELISPOT results with 94% having no detectable HCMV viral loads. All subjects (28) with a CD4+ T cell count above 100 cells/μl had undetectable HCMV viral loads. Two of the six subjects with CD4+ T cell counts <100 cells/μl had detectable HCMV viral loads. There was no statistically significant association between the CD4+ T cell counts and the HCMV IFN-γ-ELISPOT results. Conclusion: No specific point could be determined when there is loss of integrity of the HCMV-specific cellular immune response in HIV-positive individuals. Low CD4+ T cell counts did not correlate with HCMV IFN-γ-ELISPOT results suggesting that the HCMV-specific cellular immunity did not necessarily break down at low CD4+ T cell counts. Nevertheless, a CD4+ T cell count above 100 cells/μl appeared to be protective against viraemia as determined by the HCMV viral load qPCR. The IFN-γ-ELISPOT assay was employed as a tool to determine the integrity of the HCMV-specific cellular immune response in HIV-positive individuals. However, the IFN-γ-ELISPOT assay should be used in conjunction with the CD4+ T cell count and the HCMV viral load qPCR to determine when there is loss of integrity of the HCMV-specific cellular immune response and HCMV reactivation occurs. This may assist clinicians in their choice of management and appropriate pre-emptive treatment in the HIV-HCMV co-infected individual at a risk for HCMV reactivation.
AFRIKAANSE OPSOMMING: Inleiding: Heraktivering van menslike sitomegaalvirus (MSMV) in menslike immuniteitsgebreksvirus (MIV)-MSMV ko-geïnfekteerde individue kan lei tot dodelike end-orgaan siektes (EOS). Die EOS word klinies duidelik wanneer 'n kritieke aantal selle in die organe beskadig raak as gevolg van die MSMV-infeksie. Behandeling van die MSMV-verwante siekte op hierdie punt mag moontlik nie meer effektief wees nie. Daarom kan die vroeë opsporing van MSMV heraktivering nuttig wees in die gebruik van voorkomende terapie. Doel: Die doel van hierdie studie is om die punt te bepaal wanneer die MSMV-spesifieke sellulêre immuun reaksie afgebreek word met behulp van die interferon gamma (IFN-γ) ensiem-gekoppelde immunospot (ELISPOT) toets en MSMV heraktivering voorkom in MIV-positiewe, antiretrovirale terapie (ART)-naïewe individue in' n Suid-Afrikaanse instelling. Dit word gedoen in verhouding met die CD4+ T sel telling en die MSMV virale lading. Materiale en metodes: Twee-en-vyftig (52) MIV-geïnfekteerde, ART-naïewe pasiënte is vanaf primêre gesondheidsentrums, wat hul bywoon vir die behandeling van hul MIV infeksie, genader. Gehepariniseerde bloedmonsters is gebruik om die MSMV-spesifieke sellulêre immuun reaksie met behulp van die IFN-γ-ELISPOT toets en die MSMV IgG serostatus te bepaal. Etileendiamientetra-asynsuur (EDTA) bloed monsters is versamel vir die bepaling van hul CD4+ T sel telling en hul MSMV virale lading met behulp van die ―real-time‖ polimerase kettingreaksie (qPKR) waardes. Resultate: Al 52 pasiënte is bevestigde MIV-MSMV ko-infeksies, gebasseer op hul serologiese status. Die resultate van 34 pasiënte met voltooide data is ontleed. Die CD4+ T sel tellings van hierdie pasiënte het gewissel 10-784 selle/μl. Twee-en-twintig (22) (65%) pasiënte het positiewe MSMV IFN-γ-ELISPOT resultate met 94% wat ‗n negatiewe qPKR resultate. Alle pasiënte (28) met 'n CD4+ T-seltelling bo 100 selle/μl het' n negatiewe qPKR resultate. Twee van die ses pasiënte met 'n CD4+ T-seltelling <100 selle/μl het waarneembare MSMV virale ladings oor die qPKR. Daar was geen statisties beduidende assosiasie tussen die CD4+ T sel tellings en die MSMV IFN-γ-ELISPOT resultate nie. Gevolgtrekking: Geen spesifieke punt wanneer die MSMV-spesifieke sellulêre immuun reaksie afgebreek word kon in MIV-positiewe individue bepaal word nie. Lae CD4+ T sel tellings het nie ooreengestem met die MSMV IFN-γ-ELISPOT resultate nie en dui daarop dat die MSMV-spesifieke sellulêre immuniteit nie noodwendig afgebreek word teen 'n lae CD4+ T sel tellings nie. Tog blyk 'n CD4+ T-seltelling bo 100 selle/μl om beskerming teen viremie te bied. Die meriete van die gebruik van die IFN-γ-ELISPOT toets die integriteit van die MSMV-spesifieke sellulêre immuun response in MIV-positiewe individue te bepaal, is waargeneem in die opgehoopte data. Tog kan die gebruik van die IFN-γ-ELISPOT toets in samewerking met die CD4+ T sel telling en die MSMV virale lading meer voordelig in die bepaling van 'n punt wanneer die MSMV-spesifieke sellulêre immuun reaksie afbreek en herstel plaasvind. Dit kan help om klinici in hul keuse van bestuur en gepaste voorkomende behandeling in die MIV-MSMV mede-geïnfekteerde individu op 'n risiko vir herstel.
Goossens, Valère Joseph. "Cytomegalovirus mRNA transcripts and anti-cytomegalovirus antibodies as markers of the balance between cytomegalovirus and host immunity." [Maastricht : Maastricht : Universiteit Maastricht] ; University Library, Maastricht University [Host], 2003. http://arno.unimaas.nl/show.cgi?fid=6032.
Full textRawlinson, William David. "Studies of the genomes of human cytomegalovirus and murine cytomegalovirus." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308998.
Full textStuart, Persoons Maria Cornelia Johanna. "Cytomegalovirus and vascular pathology." Maastricht : Maastricht : Universiteit Maastricht ; University Library, Maastricht University [Host], 1998. http://arno.unimaas.nl/show.cgi?fid=8496.
Full textSevilla-Reyes, Edgar Enrique. "Recombination in human cytomegalovirus." Thesis, University of Glasgow, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433077.
Full textPadullés, Zamora Ariadna. "Individualització de les dosis de ganciclovir/valganciclovir per predicció bayesiana, a partir d’un model farmacocinètic poblacional en pacients transplantats d’òrgan sòlid." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/285631.
Full textA previous study of our group showed that solid organ transplant (SOT) patients treated with ganciclovir/valganciclovir following dosage manufacturer’s recommendations were either over or underexposed. The main objective of the doctoral thesis was dose adjustment by Bayesian prediction based on population pharmacokinetic model to optimize treatment according to a target value (area under the curve [AUC]=40-50µg·h/mL). Secondary objectives were: 1) developed and validate a chromatographic method coupled with ultraviolet (UV) and mass spectrometry (MS/MS) detection, 2) develop a clinically applicable limited sampling strategy (LSS) and 3) evaluate the impact of dose optimization in clinical efficacy and safety. The method UHPLC/UV and UHPLC/MS/MS were precise and accurate and the retention time was 0.7 and 0.51 min, respectively. The limit of quantification was 0.5 mg/L for UV and 0.06 mg/L for MSMS detection method. There was a good correlation between both techniques. An LSS was developed and sampling windows were designed. Predicted exposure using 3 sampling times (0.5–1.5, 4–5, and 6–8 hours) showed the best predictive performance. Finally a two arm, randomized and open-label, superiority trial (margin of 40%) in adult SOT recipients treated with ganciclovir/valganciclovir, either as prophylaxis or treatment of CMV infection, was carried out. (Group A: manufacturer’s recommended doses. Group B: dose adjustment based on target exposure by Bayesian prediction). Results of 53 SOT patients were analyzed. CGV dose adjustment by Bayesian prediction optimized exposure as the 88.4% of patients were within the therapeutic AUC range vs the 18.7% in the group following the manufacturer's recommendations, fulfilling the 40% of superiority margin (p=1.7x10-6). The time required to reach AUC was delayed in Group A (p=0.000029). A trend towards shorter time to viral clearance was seen for Group B vs group A (17.6 vs 12.5 days, p=0.125). The incidence of relapse (66.67% vs 9.01%) and the late CMV disease (33.3% vs 8.3%) were both higher in Group A vs B. No relevant differences in toxicity were observed. Results showed that dose adjustment based on target exposure by Bayesian prediction contributes to optimize the ganciclovir/valganciclovir treatment achieving the therapeutic AUC from the early stages of the treatment
Lidehäll, Anna Karin. "Cellular Immune Responses to Cytomegalovirus." Doctoral thesis, Uppsala University, Department of Oncology, Radiology and Clinical Immunology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8578.
Full textCytomegalovirus (CMV) is a widespread infection affecting 50-90% of the human population. A typical silent primary infection is followed by life-long persistence in the host under control by virus-specific CD8 (“killer”) and CD4 (“helper”) T cells. Although harmless in most people, CMV may cause disease and sequelae in patients with deficient cellular immunity, such as AIDS patients, recipients of organ transplants and children who have acquired the virus before birth. In this thesis we have characterized the cellular immunity to CMV in immunocompetent subjects, in patients receiving transplants and in infants.
In healthy individuals with latent CMV, the frequencies of CMV-specific CD8 T cells varied considerably between the donors. Within the same individual, the changes over time were usually small. In patients with primary, symptomatic CMV infection, the frequencies of CMV-specific CD8 T cells peaked within the first month after the appearance of symptoms. The frequencies then declined to levels similar to those in latently infected CMV carriers. The CD4 T-cell function followed the same pattern, but with lower peak values.
Immunosuppressed renal transplant patients with latent CMV had CMV-specific CD4 cell function similar to healthy controls. The frequencies of CMV-specific CD8 T cells were also comparable, but their function was impaired. When renal transplant recipients were investigated longitudinally, we found that their CMV-specific T cells decreased rapidly after transplantation. Whereas the frequencies and function of CD8 T cells rebounded within 3 months, CD4 T-cell recovery was impaired during the entire first year after transplantation.
Finally, the frequencies and function of CMV-specific T-cells were investigated in children with congenital and postnatal CMV. CMV-specific CD8 T cells could be detected in even the youngest children, suggesting that these cells can develop early in life. In contrast, CMV specific CD4 T cells were low or absent in the youngest children but increased slowly with age.
Odeberg, Jenny. "Human cytomegalovirus immune evasion strategies /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-126-8.
Full textLidehäll, Anna Karin. "Cellular immune responses to cytomegalovirus /." Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8578.
Full textTysoe, Carolyn. "Characterisation of human cytomegalovirus variants." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361685.
Full text勞錦輝 and Kam-fai Simon Lo. "Cytomegalovirus and bone marrow transplantation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1997. http://hub.hku.hk/bib/B31215609.
Full textKaye, Jane Frances. "Studies of human cytomegalovirus glycoproteins." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259731.
Full textBEAUDET, FAUROBERT PATRICIA. "Cytomegalovirus et maladies inflammatoires intestinales." Lyon 1, 1991. http://www.theses.fr/1991LYO1M032.
Full textCranmer, Lee D. "Experimental vaccination against murine cytomegalovirus /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1996. http://wwwlib.umi.com/cr/ucsd/fullcit?p9714856.
Full textLo, Kam-fai Simon. "Cytomegalovirus and bone marrow transplantation /." Hong Kong : University of Hong Kong, 1997. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19471142.
Full textGao, Yang. "Characterization of human cytomegalovirus UL84." abstract and full text PDF (UNR users only), 2009. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3355580.
Full textPocock, Joanna Mary. "Human cytomegalovirus and the neutrophil." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275685.
Full textHumar, Atul. "Clinical utility of quantitative cytomegalovirus viral load determination for predicting cytomegalovirus disease in liver transplant recipients." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0001/MQ45586.pdf.
Full textJames, Samantha. "Herpèsvirus de primates et de chauves-souris du nouveau monde : modèles d'étude des relations évolutives hôtes-virus DNA polymerase sequences of New World Monkey Cytomegaloviruses : another molecular marker with which to infer Platyrrhini systematics Novel herpesviruses of neotropical bats and their relationships with other members of the Herpesviridae family." Thesis, Guyane, 2019. http://www.theses.fr/2019YANE0004.
Full textViruses belonging to the Herpesviridae family (order Herpesvirales) are enveloped double-stranded DNA viruses distributed into three subfamilies: Alpha-, Beta- and Gamma-herpesvirinae. These viruses have been identified from a wide range of host species, ranging from mammals to reptiles to birds. They have the ability to persist throughout the life of the host in a latent form and to reactivate. Most of these viruses are specific to a host. The wide distribution of herpesviruses, generally associated with an asymptomatic infection in their natural host, suggests that these viruses have co-evolved with their hosts.The first part of this work was dedicated to the identification of cytomegaloviruses (CMV, genus Cytomegalovirus) in New World non-human primates (NWNHP) to see if the diversification of these viruses followed that of their hosts. A previous study on Old World primates had demonstrated that CMVs infecting them showed a strong signal of co-divergence with their hosts. Nevertheless, among the different species of primates tested, only a few were from the New World. To test this hypothesis, we performed a molecular screening of 244 blood DNA samples from 20 Central and South American species. Through a PCR approach using degenerate consensus primers targeting highly conserved motifs of the DNA polymerase gene of herpesviruses, we characterized new viral sequences from 12 species belonging to seven representative genera of the three families of NWNHP. These results demonstrate that most species of NWNHP can be infected with a virus belonging to the Cytomegalovirus genus. In addition, phylogenetic analyzes of the obtained sequences combined with their molecular dating support a co-evolutionary scenario. This study led us to propose that CMVs sequences of NWNHP could serve as a molecular marker with which to infer the not yet fully resolved systematics of these primates.The second part of this work was on identifying herpesviruses from New World bats to study their distribution and diversity. The search for herpesviruses in bats is more recent. In the early 2000s it benefited from studies dedicated to other viral families given their role as reservoirs of potentially zoonotic viruses. The first description of bat herpesvirus sequences dated back from 2007. Over the past decade, dozens of herpesvirus sequences have been described from different bat species on every continent. Nevertheless, the distribution of the tested species was geographically uneven and only a few were from the New World. Molecular screening of 195 blood DNA samples from 11 species belonging to three families (Phyllostomidae, Mormoopidae and Molossidae) was performed. Using the same approach as applied to NWNHP, we obtained viral sequences (DNA polymerase and/or glycoprotein B) from all tested species. These are distributed within the Beta- and Gamma-herpesvirinae subfamilies. Fourteen partial sequences of the DNA polymerase gene, corresponding to three beta- and eleven gamma-herpesviruses, have been identified. Twelve partial sequences of the glycoprotein B gene, all gamma-herpesviruses, have been characterized. Each sequence was specific to a bat species and in some species multiple viruses were identified. Phylogenetic analyzes of these sequences have identified clades specific to bat viruses. Those composed of sequences obtained from different species belonging to distinct subfamilies follow the taxonomy of bats. This study confirms the astonishing diversity of bat herpesviruses and broadens our knowledge on their host spectrum.This work is the largest conducted to date in terms of species diversity of non-human primates and bats from the Neotropical realm. Nevertheless, the samples tested represent only a tiny part of this diversity. Further analyzes, on a broader panel of representative species from other geographic areas will increase our understanding of the evolutionary history of these viruses
Weston, K. M. "Studies on the human cytomegalovirus genome." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384617.
Full textMcSharry, Brian. "Analysis of human cytomegalovirus gene function." Thesis, Cardiff University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400342.
Full textEllsmore, Victoria. "Human cytomegalovirus origin-dependent DNA synthesis." Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340332.
Full textChee, Mark. "Analysis of the human cytomegalovirus genome." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357727.
Full textGimeno, Brias Silvia. "Investigation of cytomegalovirus-based cancer vaccines." Thesis, Cardiff University, 2018. http://orca.cf.ac.uk/113304/.
Full textDecoene, Christophe. "Infections a cytomegalovirus et transplantation cardiaque." Lille 2, 1992. http://www.theses.fr/1992LIL2M033.
Full textLunetta, Jennine Marie. "Molecular studies of human cytomegalovirus latency /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.
Full textBuhamad, Zahrah. "Cytomegalovirus glycoprotein types and disease causation." Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/cytomegalovirus-glycoprotein-types-and-disease-causation(c05aa79c-e162-4b5b-86e7-136730243be9).html.
Full textGyurova, Ivayla E. "Phenotypic and functional dynamics of Cytomegalovirus-associated memory natural killer cells in the absence of cytomegalovirus infection." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1602152788438709.
Full textMachesky, Nicholas John. "The modulation of sphingolipids by human cytomegalovirus and its influence on viral protein accumulation and growth." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1181753517.
Full textDutra, Mauricio Cabral. "Perfil de eliminação de agentes infecciosos envolvidos em rinite na espécie suína." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-22042009-102538/.
Full textRespiratory diseases are one of the largest cause of economic losses in swine industry, it is related with grown and weight gain reduction, mortality, vaccines and medicaments costs, veterinary assistance. In that context, rinithis cases have been a major contribution. The present study propose the determination of elimination profile of agents related with rhinitis evaluating different ages in nine swine herds with history of cornet lesions and that uses different ways to control and prevent this problem. There were examined tonsils swabs from 12 animals in the following ages: sows, piglets of 20, 40 60 days and pigs of 90, 110 and 140 days, totalizing 84 pigs for farm. The swabs were searched to P. multocida capsular type A and D, dermonecrotic toxin gene from P. multocida, B. bronchiseptica and porcine cytomegalovirus through polymerase chain reaction (PCR). Despite de turbinate bones lesions present in all herds P. multocida type A was detected in only one pig and none were positive to dermonecrotic toxin gene. From 756 animals, 22 (2.9%) were positive to B. bronchiseptica and 198 (26.1%) to porcine cytomegalovirus detection. The presence of B. bronchiseptica presented statistical association with the farrowing and finishing times. Larger number of animals positive to cytomegalovirus show statistical association with the post weaning pigs. Sows carrying B. bronchiseptica in the three types of herds examined, suggesting that sows have an active participation in piglet infection by this agent. The same was not observed in porcine cytomegalovirus spread. More projects were need to clarify the low detection of P. multocida and to understand the impact of cytomegalovirus in swine production.
Kloover, Jeroen Steven. "Experimental cytomegalovirus infections in a rat model pathogenesis and treatment /." [Maastricht : Maastricht : Universiteit Maastricht] ; University Library, Maastricht University [Host], 2002. http://arno.unimaas.nl/show.cgi?fid=7075.
Full textZolnourian, Z. "Molecular approaches for the detector of opportunistic infectious agents." Thesis, Queen's University Belfast, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269151.
Full textCoaquette, Alain. "Donnees actuelles de l'infection a cytomegalovirus : implications virologiques et cliniques." Besançon, 1990. http://www.theses.fr/1990BESA3082.
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