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Journal articles on the topic 'Cytochemistry'

To see the other types of publications on this topic, follow the link: Cytochemistry.

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Published: 4 June 2021

Last updated: 4 March 2023

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1

Takizawa, T., and T. Saito. "Freeze-Fracture Enzyme Cytochemistry: Application of Enzyme Cytochemistry to Freeze-Fracture Cytochemistry." Journal of Electron Microscopy 45, no. 3 (June 1, 1996): 242–46. http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023440.

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2

Cuevas, P., J. A. Gutierrez-Diaz, D. Reimers, M. Dujovny, F. G. Diaz, and J. I. Ausman. "Intramembranous cytochemistry." Neurosurgery 20, no. 2 (February 1987): 243???8. http://dx.doi.org/10.1097/00006123-198702000-00008.

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3

Farhi, Diane C. "Haematological Cytochemistry." American Journal of Clinical Pathology 104, no. 2 (August 1, 1995): 230.2–230. http://dx.doi.org/10.1093/ajcp/104.2.230a.

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4

Kitazawa, Sohei, Teruyuki Ohno, Ryuma Haraguchi, and Riko Kitazawa. "Histochemistry, Cytochemistry and Epigenetics." ACTA HISTOCHEMICA ET CYTOCHEMICA 55, no. 1 (February 26, 2022): 1–7. http://dx.doi.org/10.1267/ahc.21-00095.

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5

Amiry-Moghaddam, Mahmood, and Ole Petter Ottersen. "Immunogold cytochemistry in neuroscience." Nature Neuroscience 16, no. 7 (June 25, 2013): 798–804. http://dx.doi.org/10.1038/nn.3418.

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6

Roels, F., B. De Prest, and G. De Pestel. "Liver and chorion cytochemistry." Journal of Inherited Metabolic Disease 18, S1 (January 1995): 155–71. http://dx.doi.org/10.1007/bf00711437.

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7

Gossrau, R. "Cytochemistry of membrane proteases." Histochemical Journal 17, no. 7 (July 1985): 737–71. http://dx.doi.org/10.1007/bf01003312.

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8

Neame, PB, P. Soamboonsrup, GP Browman, RM Meyer, A. Benger, WE Wilson, IR Walker, N. Saeed, and JA McBride. "Classifying acute leukemia by immunophenotyping: a combined FAB- immunologic classification of AML." Blood 68, no. 6 (December 1, 1986): 1355–62. http://dx.doi.org/10.1182/blood.v68.6.1355.1355.

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Abstract A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative orinconclusive cytochemistry. At present, we suggest that until a “gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.

9

Neame, PB, P. Soamboonsrup, GP Browman, RM Meyer, A. Benger, WE Wilson, IR Walker, N. Saeed, and JA McBride. "Classifying acute leukemia by immunophenotyping: a combined FAB- immunologic classification of AML." Blood 68, no. 6 (December 1, 1986): 1355–62. http://dx.doi.org/10.1182/blood.v68.6.1355.bloodjournal6861355.

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A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative orinconclusive cytochemistry. At present, we suggest that until a “gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.

10

Takizawa, Toshihiro. "5′-Nucleotidase in Rat Photoreceptor Cells and Pigment Epithelial Cells Processed by Rapid-freezing Enzyme Cytochemistry." Journal of Histochemistry & Cytochemistry 46, no. 9 (September 1998): 1091–95. http://dx.doi.org/10.1177/002215549804600914.

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This report describes the subcellular distribution of 5′-nucleotidase (5′-NT) in rat photoreceptor cells and pigment epithelial cells processed by rapid-freeze enzyme cytochemistry. There was a striking difference in the ultrastructural localization of 5′-NT activity between rod outer segments after freeze-substitution fixation and conventional fixation. By rapid-freezing enzyme cytochemistry, 5′-NT activity was localized in the extradiscal space of intact nonvacuolated discs, whereas by conventional cytochemistry it was shown in the intradiscal space of artifactual vacuolated discs. In the freeze-substituted retinal cells, an appreciable difference in functional 5′-NT molecules was also found. The soluble 5′-NT on the cytoplasmic side of the disc membrane was vital in the rod outer segments, whereas the membrane-bound ecto-5′-NT on the exoplasmic (external) surface of the apical process was active in the pigment epithelial cells. Rapid-freezing enzyme cytochemistry should be worth employing as a method to reveal the fine localization of enzyme activity at the level of cell ultrastructures, which are poorly preserved by conventional fixation, and should provide information approximate to that in living cells.

11

Takizawa, Toshihiro, Takuma Saito, and John M. Robinson. "Freeze-fracture Cytochemistry: A New Method Combining Immunocytochemistry and Enzyme Cytochemistry on Replicas." Journal of Histochemistry & Cytochemistry 46, no. 1 (January 1998): 11–17. http://dx.doi.org/10.1177/002215549804600103.

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We describe a new freeze-fracture cytochemical technique consisting of combined immunocytochemistry and enzyme cytochemistry. This technique reveals the relationship between molecules in biological membranes by double labeling with two different cytochemical markers (i.e., immunogold probes and cerium). In this method, antigens were detected with specific primary antibodies and appropriate secondary immunoprobes. Subsequently, alkaline phosphatase activity was detected with cerium as the capture agent on the same replicas. Octyl-glucoside (OG) digestion before the cytochemical reactions was crucial to the success of this combined method. OG is an efficient detergent and OG digestion can preserve both immunocytochemical antigenicity and enzyme activity on replicas. As an initial examination, we applied this technique to the study of glycosyl-phosphatidyl-inositol-anchored proteins and adhesion molecules in human neutrophils. The method described here should serve as a unique additional approach for the study of topology and dynamics of molecules in biomembranes.

12

Bendix-Hansen, Knud. "ENZYME CYTOCHEMISTRY OF NEUTROPHIL GRANULOCYTES." British Journal of Haematology 65, no. 2 (February 1987): 127–29. http://dx.doi.org/10.1111/j.1365-2141.1987.tb02253.x.

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13

Fitzsimrnons, E. I. "Leukaemia Cytochemistry-Principles and Practice." Histopathology 16, no. 3 (March 1990): 313–14. http://dx.doi.org/10.1111/j.1365-2559.1990.tb01127.x.

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14

Mizuhira, V., and H. Hasegawa. "Microwave fixation method for cytochemistry." European Journal of Morphology 34, no. 5 (December 1, 1996): 385–92. http://dx.doi.org/10.1076/ejom.34.5.385.13055.

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15

Weis, Kitren G., Karin R. Jacobsen, and Judith A. Jernstedt. "Cytochemistry of developing cotton fibers:." Field Crops Research 62, no. 2-3 (June 1999): 107–17. http://dx.doi.org/10.1016/s0378-4290(99)00004-0.

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16

Nepi, M., and G. G. Franchi. "Cytochemistry of mature angiosperm pollen." Plant Systematics and Evolution 222, no. 1-4 (2000): 45–62. http://dx.doi.org/10.1007/bf00984095.

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17

Coulton, Gary. "Are histochemistry and cytochemistry 'Omics'?" Histochemical Journal 35, no. 6 (August 2004): 603–13. http://dx.doi.org/10.1007/s10735-004-2193-7.

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18

Tamaki, H., and S. Yamashina. "Improved method for post-embedding cytochemistry using reduced osmium and LR white resin." Journal of Histochemistry & Cytochemistry 42, no. 9 (September 1994): 1285–93. http://dx.doi.org/10.1177/42.9.8064136.

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We established an improved method for post-embedding cytochemistry by which highly specific cytochemical reactions on excellent cellular ultrastructures are possible. The method is a combination of post-fixation in potassium ferrocyanide-reduced OsO4 and embedment in acrylic-based LR White resin. It permits both immuno- and lectin-gold cytochemistry with fine ultrastructures comparable to those obtained by conventionally osmicated and epoxy-embedded tissues. Fixation with reduced osmium appeared to contribute to the preservation of immunoreactivity and membranous structures. By this method, the immunocytochemical localization of secretory proteins (amylase and chymotrypsinogen), actin filaments by polyclonal antibodies, 105 KD Golgi-associated protein by a monoclonal antibody (GF-1), and binding sites for gold-labeled lectin could be demonstrated. Also possible was multiple staining with enzyme cytochemistry of thiamine pyrophosphatase and immunocytochemistry of GF-1 and anti-amylase antibodies. This multiple staining made possible partial characterization of the trans-Golgi network in parotid acinar cells.

19

Kan, Frederick W. K. "Applications of freeze-fracture cytochemistry as applied to the study of tissues and cells." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 1006–9. http://dx.doi.org/10.1017/s0424820100129280.

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The applications of freeze-fracture cytochemistry to the study of tissues and cells are described. Two approaches will be presented: label-fracture and fracture-label. In both techniques, colloidal gold probe is used as the marker system. The use of cytochemistry in combination with freeze-fracture for the study of distribution of receptor sites, antigens and glyco- conjugates on freeze-fractured plasma membranes as well as on fractured intracellular organelles is illustrated respectively by the two models described below. A variant of the fracture-label technique, Backscattered Electron Imaging of labeled tissues following freeze-fracture cytochemistry, is also introduced as a novel method for the 3-dimensional visualization of the in situ distribution of receptor sites on freeze-fractured membranes.1.Molecular demarcation of surface domains in boar spermatozoa as visualized by label-fracture cytochemistry.The label-fracture technique has been previously described. In brief, boar sperm cells are fixed, labeled and impregnated with glycerol.

20

Chatterjee, Tathagata, Manoranjan Mahapatra, Hara P. Pati, Inusha Panigrahi, Rajat Kumar, Shashi Wadhwa, and Renu Saxena. "Use of Transmission Electron Microscopy in Diagnosis of Acute Leukemias: A Prospective Study of Fifty Cases." Blood 106, no. 11 (November 16, 2005): 4509. http://dx.doi.org/10.1182/blood.v106.11.4509.4509.

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Abstract Ultrastructural studies have contributed substantially to the understanding of the cellular morphology of the acute leukemias. We present here 50 cases (aged 3-55 years, M: F-32: 18) of acute leukemias, which were studied for morphology, conventional cyto chemistry, immunophenotyping, and transmission electron microscopy (TEM) including ultrastructural cytochemistry using myeloperoxidase (MPO) and platelet peroxidase activity. TEM morphology using ultrastructural cytochemistry of MPO helped diagnose three cases of acute myeloid leukemia with minimal myeloid differentiation (AML M0). The blasts showed large round nuclei, 1–2 nucleoli, chromatin with peripheral condensation and abundant mitochondria. Of total of 5 cases of acute promyelocytic leukemias (APML), all had strong Sudan Black, MPO, dual esterase positivity; one case was non-specific esterase positive and sensitive to fluoride. On TEM, this unusual case was identified to be microgranular variant of APML. TEM morphology and ultrastructural cytochemistry using platelet peroxidase helped diagnose 3 cases of AML M7 (acute megakaryocytic leukemia), 2 cases of acute biphenotypic leukemias and also in differentiating one case of acute proerythroblastic leukemia (AML M6b) from 3 cases of AML M6a or acute erythroleukemia. Thus, TEM is helpful in differentiating further the subgroups of AML-M5 and AML-M6, in identifying the microgranular variant of APML, and in confirming the diagnosis of AML-M0 and biphenotypic leukemia. Also, in cases with very hypercellular marrow and with associated myelofibrosis, where the bone marrow aspiration gives low cell count, TEM and ultrastructural cytochemistry are a valuable aid to arriving at a accurate diagnosis. Characteristics of acute leukemia cases (n=50) FAB Subtype Morphology, Conventional Cytochemistry and Immunophenotyping Transmission Electron Microscopy (?) = doubt in diagnosis AML-M0 3 (?) 3 AML-M1and M2 11 11 AML-M3 4, 1(?) 5: Hypergranular-4, hypogranular-1 AML-M4 5 5 AML-M5 5 5a-4, 5b-1 AML-M6 4 6a-3 (erythroleukemia), 6b-1 AML-M7 3 3 Biphenotypic 2 (?) 2 ALL-L1 8 8 ALL-L2 4 4

21

Pargney, Jean-Claude. "Essais de caractérisation cytochimique des structures de l'interface au niveau du réseau de Hartig dans l'association ectomycorhizienne entre la truffe (Tuber melanosporum) et le noisetier (Corylus avellana)." Canadian Journal of Botany 68, no. 12 (December 1, 1990): 2722–28. http://dx.doi.org/10.1139/b90-345.

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The interface established between Tuber melanosporum and Corylus avellana was studied cytochemically (PATAg test, Swift's reaction, wheat germ agglutinin – colloidal gold labelling) to characterize cell wall and matrix components. By combining ultrastructural cytochemistry and selective extractions of polysaccharides by various solvents (EDTA, dimethyl sulfoxide, methylamine) or enzymes (pectinase, cellulase, cytohelicase), some ultrastructural features were made evident. Ultrastructural cytochemical tests demonstrate different domains in the matrix. Cell wall and matrix components are similar, but the fungal chitin is not detected in the matrix. Key words: ectomycorrhiza, interface, ultrastructural cytochemistry, selective extractions.

22

MASUZAWA, Toshio, Toshiko OHTA, and Fumiaki SATO. "Enzyme Cytochemistry of Ventricular Choroid Plexus." Neurologia medico-chirurgica 25, no. 11 (1985): 881–85. http://dx.doi.org/10.2176/nmc.25.881.

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23

Kurosawa, Hidemitsu, Mitsuoki Eguchi, Hitoshi Sakakibara, Hiroshi Takahashi, and Toshiharu Furukawa. "Ultrastructural Cytochemistry of Congenital Basophilic Leukemia." Journal of Pediatric Hematology/Oncology 9, no. 1 (1987): 27–32. http://dx.doi.org/10.1097/00043426-198721000-00006.

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24

Glick, D. "Fifty years with histochemistry and cytochemistry." Journal of Histochemistry & Cytochemistry 33, no. 7 (July 1985): 720–28. http://dx.doi.org/10.1177/33.7.2409132.

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25

Vrčić, Hrvoje, Božidar Horvat, and Ivan Damjanov. "Lectin Cytochemistry of Mouse Vaginal Smears." Gynecologic and Obstetric Investigation 33, no. 2 (1992): 69–74. http://dx.doi.org/10.1159/000294851.

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26

Ishiyama, Gail, Ivan A. Lopez, Ali R. Sepahdari, and Akira Ishiyama. "Meniere's disease: histopathology, cytochemistry, and imaging." Annals of the New York Academy of Sciences 1343, no. 1 (March 12, 2015): 49–57. http://dx.doi.org/10.1111/nyas.12699.

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27

Yamashita, Shuji. "Histochemistry and Cytochemistry of Nuclear Receptors." Progress in Histochemistry and Cytochemistry 36, no. 2 (January 2001): 91–176. http://dx.doi.org/10.1016/s0079-6336(01)80004-8.

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28

Holland, G. R. "Nociceptor sprouting and cytochemistry in injury." Pathophysiology 5 (June 1998): 164. http://dx.doi.org/10.1016/s0928-4680(98)80929-2.

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29

da Silva, Pedro Pinto. "Molecular cytochemistry of freeze-fractured cells." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 894–97. http://dx.doi.org/10.1017/s0424820100145807.

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I will describe four approaches that combine cytochemistry with freeze-fracture: 1) FREEZE-ETCHING; 2) FRACTURE-LABEL; 3) FRACTURE-PERMEATION; and 4) LABEL-FRACTURE. These techniques, in particular fracture-label, involve delicate points of interpretation and numerous validating controls. In the publications listed at the end, these issues have been addressed in detail.1. FREEZE-ETCHING. I developed freeze-etching as a cytochemical approach to prove that membranes were split by freeze-fracture and to show that biological membranes were comprised of a bilayer membrane continuum interrupted by integral membrane proteins.1 - 4 In freeze-etching, the distribution of the marker over the membrane surface exposed by sublimation is compared to that of the intramembrane particles exposed by fracture. It is often required to aggregate the particles into domains larger than the labeling molecules (Fig. 1). This, and the need for freezing in distilled water, severely limits the application of freeze-etching.

30

Li, Chin-Yang, and Lung T. Yam. "Cytochemistry and Immunochemistry in Hematologic Diagnoses." Hematology/Oncology Clinics of North America 8, no. 4 (August 1994): 665–81. http://dx.doi.org/10.1016/s0889-8588(18)30153-9.

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31

Van Noorden, Cornelis J. F. "Molecular probes in histochemistry and cytochemistry." Acta Histochemica 100, no. 4 (November 1998): 337. http://dx.doi.org/10.1016/s0065-1281(98)80030-5.

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32

Andersson Forsman, C. "Freeze-fracture cytochemistry of sympathetic ganglia." Histochemistry 82, no. 3 (1985): 209–18. http://dx.doi.org/10.1007/bf00501397.

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33

Ploem, J. S., A. M. J. van Driel-Kulker, L. Goyarts-Veldstra, J. J. Ploem-Zaaijer, N. P. Verwoerd, and M. van der Zwan. "Image analysis combined with quantitative cytochemistry." Histochemistry 84, no. 4-6 (July 1986): 549–55. http://dx.doi.org/10.1007/bf00482990.

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34

Fujimoto, K., K. S. Ogawa, and K. Ogawa. "Gastric K+-stimulated p-nitrophenylphosphatase cytochemistry." Histochemistry 84, no. 4-6 (1986): 600–608. http://dx.doi.org/10.1007/bf00482998.

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35

VANMEIR, F., and D. SCHEUERMANN. "Catalase cytochemistry of interalveolar septal cells." Cell Biology International Reports 14 (September 1990): 212. http://dx.doi.org/10.1016/0309-1651(90)90949-y.

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36

Moore, Michael N. "Lysosomal cytochemistry in marine environmental monitoring." Histochemical Journal 22, no. 4 (April 1990): 187–91. http://dx.doi.org/10.1007/bf02386003.

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37

Sanna, Pietro Paolo, Gustav F. Jirikowski, Gail A. Lewandowski, and Floyd E. Bloom. "Applications of DAPI Cytochemistry to Neurobiology." Biotechnic & Histochemistry 67, no. 6 (January 1992): 346–50. http://dx.doi.org/10.3109/10520299209110047.

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38

Gahan, P. B. "Quantitative enzyme cytochemistry in plant biotechnology." Phytochemical Analysis 2, no. 3 (July 1991): 97–106. http://dx.doi.org/10.1002/pca.2800020302.

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39

Beesley, J. E. "Immunolabelling and electron microscopy in cytochemistry." Current Opinion in Immunology 2, no. 6 (January 1989): 927–31. http://dx.doi.org/10.1016/0952-7915(89)90180-5.

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40

Severs, Nicholas J. "Freeze-fracture cytochemistry: Review of methods." Journal of Electron Microscopy Technique 13, no. 3 (November 1989): 175–203. http://dx.doi.org/10.1002/jemt.1060130306.

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41

Willis, Clinton, Johanna Nyffeler, and Joshua Harrill. "Phenotypic Profiling of Reference Chemicals across Biologically Diverse Cell Types Using the Cell Painting Assay." SLAS DISCOVERY: Advancing the Science of Drug Discovery 25, no. 7 (June 17, 2020): 755–69. http://dx.doi.org/10.1177/2472555220928004.

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Cell Painting is a high-throughput phenotypic profiling assay that uses fluorescent cytochemistry to visualize a variety of organelles and high-content imaging to derive a large number of morphological features at the single-cell level. Most Cell Painting studies have used the U-2 OS cell line for chemical or functional genomics screening. The Cell Painting assay can be used with many other human-derived cell types, given that the assay is based on the use of fluoroprobes that label organelles that are present in most (if not all) human cells. Questions remain, however, regarding the optimization steps required and overall ease of deployment of the Cell Painting assay to novel cell types. Here, we used the Cell Painting assay to characterize the phenotypic effects of 14 phenotypic reference chemicals in concentration–response screening mode across six biologically diverse human-derived cell lines (U-2 OS, MCF7, HepG2, A549, HTB-9 and ARPE-19). All cell lines were labeled using the same cytochemistry protocol, and the same set of phenotypic features was calculated. We found it necessary to optimize image acquisition settings and cell segmentation parameters for each cell type, but did not adjust the cytochemistry protocol. For some reference chemicals, similar subsets of phenotypic features corresponding to a particular organelle were associated with the highest-effect magnitudes in each affected cell type. Overall, for certain chemicals, the Cell Painting assay yielded qualitatively similar biological activity profiles among a group of diverse, morphologically distinct human-derived cell lines without the requirement for cell type–specific optimization of cytochemistry protocols.

42

Robinson, J. M., and M. J. Karnovsky. "Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity." Journal of Histochemistry & Cytochemistry 39, no. 6 (June 1991): 787–92. http://dx.doi.org/10.1177/39.6.2033237.

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We show that tubular structures present in phorbol ester-stimulated macrophages are sensitive to commonly used chemical fixatives (i.e., they usually become fragmented during fixation). These structures are well preserved in macrophages that are physically fixed by rapid-freezing and subsequent freeze-substitution in osmium-acetone. We have developed methods that combine rapid-freezing, freeze-substitution, and enzyme cytochemistry for preservation of these tubular structures and for detection of endocytosed material (i.e., horseradish peroxidase). This method of rapid-freeze cytochemistry may be useful in other situations where chemical fixation does not adequately preserve cell structures, particularly of membrane compartments.

43

Rendayan, Moise. "Facts and artifacts in colloidal gold postembedding cytochemistry." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 44–47. http://dx.doi.org/10.1017/s0424820100141962.

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The colloidal gold marker was introduced in immunocytochemistry by Faulk and Taylor, in 1971, for the ultrastructural localization of surface antigens. Since then, application of this marker in light and electron microscopy has been growing rapidly. In particular, it has been applied for postembedding labeling of intracellular binding sites and its use has been extended to the various fields of cytochemistry: immunocytochemistry (protein A-gold), IgG-gold), enzyme-cytochemistry and lectincytochemistry. Several reviews have been recently published on colloidal gold labeling techniques and we refer to them for extensive characterization of this marker and its various applications.

44

Arshad A., Abu, Narmatha M., and Ulaganathan S. "HEMATOLOGICAL AND CLINICAL EVALUATION OF LEUKEMIAS, USING CYTOCHEMICAL STAINS AND IMMUNOPHENOTYPING." International Journal of Advanced Research 10, no. 09 (September 30, 2022): 973–78. http://dx.doi.org/10.21474/ijar01/15444.

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Background: Leukemias are abnormal proliferation of hematopoietic cells, causing progressive infiltration of the marrow. It is the eleventh most common cancer in the world, and increasingly found now in developing countries. Two widely used classifications are used now, the FAB, and the WHO classification, which has got supplanted now, with increasing knowledge on cytomorphology and cytogenetics. This study, attempts to evaluate the role of cytochemistry as a cost-effective tool, in the various types of leukemias, and the role of immunophenotyping in a select few cases. Aim:- The main aim of the study, was to assess the type, and subtype of leukemia, using cytochemistry, and to find their concordance with immunophenotyping in a select few cases, as a cost effective tool in diagnosing it. Methods: 56 cases of leukemia, were identified by morphology and cytochemistry, using Sudan black B, and PAS stains. Immunophenotyping, was done in 6 cases selectively, and their concordance rate was determined. Results:- Out of 56 cases of leukemia, 36 were acute, rest 20, were chronic cases. AML, accounted for 43% of the cases, CML at 33%, and ALL at 22%. Anemia was seen, more in acute leukemias, especially ALL, followed by AML. Total count values were seen high in CML, followed by AML. Platelet counts, were less in acute leukemias, especially ALL,followed by lowest in AML. Splenomegaly, was the commonest feature seen in 21 cases. Immunophenotyping, was done in 6 cases, 4 cases were concordant, showing a 67% rate. Conclusion :- In a setting where there is a lack of facilities for flow cytometry, as in the developing countries, morphology combined with cytochemistry, still serves as the best means in diagnosing leukemia cases.

45

Invernizzi, R., R. Nano, O. Perugini, P. Fazio, L. Nespoli, G. Gerzeli, and E. Ascari. "Tetrahydrofolate dehydrogenase cytochemistry in acute lymphoblastic leukemia." European Journal of Haematology 41, no. 2 (April 24, 2009): 109–14. http://dx.doi.org/10.1111/j.1600-0609.1988.tb00879.x.

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46

Derenzini, M., M. Thiry, and G. Goessens. "Ultrastructural cytochemistry of the mammalian cell nucleolus." Journal of Histochemistry & Cytochemistry 38, no. 9 (September 1990): 1237–56. http://dx.doi.org/10.1177/38.9.2201735.

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Abstract:

In the present review on the organization of the mammalian cell nucleolus, we report and discuss data obtained during the past 10 years by means of cytochemical and immunocytochemical ultrastructural techniques. Particular emphasis is placed on the following topics: location of the nucleolus organizer regions in interphasic nucleolar components, structure of nucleolar chromatin in situ, and the structure-function relationship of the nucleolar components. The cytochemical and immunocytochemical results are compared and the concordant data are stressed for each topic.

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Maraldi, N. M., N. Zini, S. Squarzoni, R. Del Coco, P. Sabatelli, and F. A. Manzoli. "Intranuclear localization of phospholipids by ultrastructural cytochemistry." Journal of Histochemistry & Cytochemistry 40, no. 9 (September 1992): 1383–92. http://dx.doi.org/10.1177/40.9.1506675.

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Abstract:

The presence of phospholipids within the interphase nucleus and in isolated chromatin, previously demonstrated by analytical biochemical methods, has been only rarely documented by cytochemical procedures, especially at the ultrastructural level. By means of a gold-conjugated phospholipase technique, we investigated the fine localization of endogenous phospholipids in the different nuclear domains in rat pancreas and in cell cultures. To reduce possible removal or displacement of phospholipids, different specimen preparation procedures such as cryofixation, cryosectioning, and freeze-fracturing were utilized. Apart from slight differences in efficiency among these methods, phospholipids have been cytochemically identified in the same nuclear domains: the interchromatin granules and fibers and the dense fibrillar component of the nucleolus. These results suggest that the phospholipids are an actual nuclear component, not randomly distributed in the nucleoplasm but mainly localized in the nuclear domains involved in the synthesis, maturation, and transport of ribonucleoproteins.

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Burry, Richard W., and Denis G. Baskin. "The Online Journal of Histochemistry and Cytochemistry." Journal of Histochemistry & Cytochemistry 46, no. 1 (January 1998): 1–2. http://dx.doi.org/10.1177/002215549804600101.

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Bojović-Cvetić, Dubravka, and Radmila Vujičić. "Polysaccharide cytochemistry in maturing Aspergillus flavus sclerotia." Transactions of the British Mycological Society 91, no. 4 (December 1988): 619–24. http://dx.doi.org/10.1016/s0007-1536(88)80036-6.

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Stussi-Garaud, Christiane, and Odette Rohfritsch. "Methods in Plant Electron Microscopy and Cytochemistry." Plant Science 160, no. 4 (March 2001): 753–54. http://dx.doi.org/10.1016/s0168-9452(00)00434-9.

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