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1

Dynowska, Maria. "Variability of selerotia in some species of genus Typhula." Acta Mycologica 24, no. 2 (August 20, 2014): 193–99. http://dx.doi.org/10.5586/am.1988.014.

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On the basis of <i>Typhula inearnata</i> Lasch ex Fr., <i>Typhula phacorrhiza</i> Fr. and <i>Typhula variabilis</i> Riess. sclerotia morphology comparison the dependence of their structure upon the conditions in which they come into existence has boen found. The sclerotia show a larger variability in natural environment than in culture. The principle differences lie in the structure of enveloping layers: epidermoid, cuticle and the cuter part of bark.
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2

Fotios, S., and T. Gado. "Authors’ response to C Cuttle." Lighting Research & Technology 37, no. 2 (June 2005): 130–31. http://dx.doi.org/10.1177/136578280503700208.

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3

Hillerton, J. Eric, and Julian F. V. Vincent. "In vitro Aggregation of Proteins from Insect Cuticle." Entomologia Generalis 11, no. 1-2 (December 1, 1985): 1–9. http://dx.doi.org/10.1127/entom.gen/11/1985/1.

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4

Chassot, Celine, Christiane Nawrath, and Jean-Pierre Metraux. "The cuticle." Plant Signaling & Behavior 3, no. 2 (February 2008): 142–44. http://dx.doi.org/10.4161/psb.3.2.5071.

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5

Czerneková, Michaela, and Stanislav Vinopal. "The tardigrade cuticle." Limnological Review 21, no. 3 (September 1, 2021): 127–46. http://dx.doi.org/10.2478/limre-2021-0012.

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Abstract Tardigrades (phylum Tardigrada) are aquatic microecdysozoans that have adapted to survive extreme conditions through the formation of cysts or ametabolic tuns. Their body is covered by a cuticle that plays an important role in their life cycle, including their response and adaptation to environmental challenges. Cuticular characteristics are a critical component of tardigrade taxonomy. Therefore, research has often been focused on the relationship between cuticular morphology and ultrastructure and the evolutionary and phylogenetic positioning of the phylum and individual species herein. However, a deeper insight into the ultrastructural characteristics and chemical composition of the tardigrade cuticle is needed. This knowledge is important not only for a better understanding of tardigrade physiology and ecology but also for the development of efficient microinjection and/or electroporation techniques that would allow for genetic manipulation, opening new avenues in tardigrade research. Here, we review data on cuticle ultrastructure and chemical composition. Further, we discuss how the cuticle is affected during moulting, encystment, cyclomorphosis, and anhydrobiosis. Our work indicates that more systematic studies on the molecular composition of the tardigrade cuticle and on the process of its formation are needed to improve our understanding of its properties and functions.
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6

Moussu, Steven, Rita San-Bento, Roberta Galletti, Audrey Creff, Etienne Farcot, and Gwyneth Ingram. "Embryonic cuticle establishment." Plant Signaling & Behavior 8, no. 12 (December 2013): e27491. http://dx.doi.org/10.4161/psb.27491.

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7

Van Eetvelde, L., K. Chiers, and L. Van Brantegem. "Caniene cutane mastceltumoren." Vlaams Diergeneeskundig Tijdschrift 86, no. 5 (October 30, 2017): 311–21. http://dx.doi.org/10.21825/vdt.v86i5.16171.

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Caniene cutane mastceltumoren (cMCT) zijn een vaak voorkomende neoplasie in de eerstelijnspraktijk. In 96% van de gevallen kunnen cMCT worden gediagnosticeerd via cytologie. Het stellen van een prognose is echter geen evidentie omwille van hun variabel biologisch karakter. Aan de hand van verschillende factoren, zoals tumorlocatie, de aanwezigheid van systemische klachten en metastasen, histologische en cytologische gradering, proliferatiemerkers, KIT-lokalisatiepatroon, KIT-mutatie en de tumorvrije randen, wordt de prognose ingeschat. De gekozen behandeling is gebaseerd op het resultaat van deze prognostische factoren, het klinisch stadium en de lokalisatie van de tumor. Mogelijke behandelingen zijn chirurgie, radiotherapie, chemotherapie, elektrochemotherapie, tyrosine-kinasereceptorinhibitor, cryotherapie en intraregionale behandeling met gedeïonizeerd water.
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8

Carr, Herman Y., and Robert V. Pound. "Henry Cutler Torrey." Physics Today 51, no. 10 (October 1998): 100–102. http://dx.doi.org/10.1063/1.2805908.

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9

Hadley, Neil F. "The Arthropod Cuticle." Scientific American 255, no. 1 (July 1986): 104–12. http://dx.doi.org/10.1038/scientificamerican0786-104.

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10

Johnstone, Iain L. "Cuticle collagen genes." Trends in Genetics 16, no. 1 (January 2000): 21–27. http://dx.doi.org/10.1016/s0168-9525(99)01857-0.

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11

Hopkins, T. L., and K. J. Kramer. "Insect Cuticle Sclerotization." Annual Review of Entomology 37, no. 1 (January 1992): 273–302. http://dx.doi.org/10.1146/annurev.en.37.010192.001421.

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12

Cutler, C. "Response from cutler." Trends in Microbiology 3, no. 4 (April 1995): 148. http://dx.doi.org/10.1016/s0966-842x(00)88905-2.

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13

Bauer, Joan. "Cute? … or “Cutesy”?" Journal of PeriAnesthesia Nursing 11, no. 3 (June 1996): 199–200. http://dx.doi.org/10.1016/s1089-9472(96)90025-8.

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14

Bauer, Joan. "Cute? … or “Cutesy”?" Journal of PeriAnesthesia Nursing 20, no. 5 (October 2005): 309–10. http://dx.doi.org/10.1016/j.jopan.2005.09.011.

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15

Hooper, Rowan. "World's cutest spiders." New Scientist 225, no. 3013 (March 2015): 30–31. http://dx.doi.org/10.1016/s0262-4079(15)60550-4.

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16

Fotios, S. "Author’s response to S Berman and C Cuttle." Lighting Research & Technology 33, no. 3 (September 2001): 181. http://dx.doi.org/10.1177/136578280103300309.

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17

Parpairi, K., NV Baker, KA Steemers, and R. Compagnon. "Authors’ response to C Cuttle and D Loe." Lighting Research & Technology 34, no. 1 (March 2002): 67–68. http://dx.doi.org/10.1177/136578280203400118.

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18

Jay, PA. "Author’s response to C Cuttle and RG Venning." Lighting Research & Technology 34, no. 2 (June 2002): 98–99. http://dx.doi.org/10.1177/136578280203400205.

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19

Millay, Michael A., Thomas N. Taylor, and Edith L. Taylor. "Phi Thickenings in Fossil Seed Plants from Antarctica." IAWA Journal 8, no. 3 (1987): 191–201. http://dx.doi.org/10.1163/22941932-90001046.

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Primary anatomy and secondary development is described for two root types from the Fremouw Peak locality (Transantarctic Mts, Antarctica) of early to middle Triassic age. Roots of Antarcticycas have a bilayered cortex with thick surface cuticle, diarch xylem, and a clearIy defined endodermis surrounded by a single cell layer possessing phi thickenings. Secondary development begins with phellern and phelloderm production from the out er primary phloem position, and is followed bya bifacial vascular cambium next to the primary xylem that pro duces sieve cells and ray parenchyma to the outside. Young roots of Antarcticoxylon are similar to those of Antarcticycas, but may possess 2-3 cell layers with phi thickenings. Secondary development from a bifacial vascular cambium produces alternating bands of sieve cells and phloem parenchyma cells in the secondary phloem and wood with uniseriate rays and scattered axial parenchyma. The presence of phi thickenings and an epidermal cutieie in both roots suggests environmental stress related to water regulation. The occurrence of phi thickenings in the roots of some conifers, angiosperms, a fossil cycad and a probable seed fern suggests this character is of ecological rather than phylogenetic significance.
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20

Szczuka, Ewa, and Bohdan Rodkiewicz. "A cutin fluorescence pattern in developing embryos of some angiosperms." Acta Societatis Botanicorum Poloniae 65, no. 1-2 (2014): 155–60. http://dx.doi.org/10.5586/asbp.1996.027.

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A cuticle visualized by auramine O fluorescence appears on the developing embryos of 9 species belonging to <em>Cruciferae</em>, <em>Caryophyllaceae</em>, <em>Plantaginaceae</em>, <em>Linaceae</em> and <em>Papilionaceae</em>. In the investigated species the formation and extent of fluorescing and non-fluorescing embryonic areas follow a similar pattern. At first the cutin fluorescing layer is formed on the apical part of the proembryo without delimited protoderm. This layer extends and at the late globular stage envelops the embryo proper, except for a cell adjoining the suspensor. Fluorescing cutin persists during the heart stage but disappears from the torpedo embryo. During these stages there is no cutine fluorescence on suspensorial cells. Continuous cutin fluorescence appears again on the surface of the whole embryo by the late torpedo stage. Then fluorescence disappears from the radicular part of U-shaped embryos, but persists on the shoot apex, cotyledons and at least on the upper part of hypocotyl. It is assumed that polarization and nutrition of the embryo may be influenced by cuticular changes.
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21

Al-Amin, Mohammad, M. H. Uddin, A. Afrin, K. B. Nath, and S. Barua. "Extraction, Physico-Chemical Characterization and Antimicrobial Screening of the Muscle Lipid of Cuttle Fish (Sepia esculenta) of the Bay of Bengal." International Letters of Chemistry, Physics and Astronomy 36 (July 2014): 87–97. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.36.87.

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Lipid was extracted from the muscle of Cuttle fish by solvent extraction method and then purified by suitable conventional method. Various physical constants e.g. refractive index, viscosity, specific gravity, crude fat, crude fibre and ash contents and chemical parameters e.g. saponification value, saponification equivalent value, acid value, iodine value, acetyl value, peroxide value, thiocyanogen value, Reichert-Meissl value, Polenske value, Henher value, cholesterol content etc. of the lipid sample have been determined and compared with those of different standard oils. Fatty acids composition of the sample was investigated by Thin Layer Chromatography (TLC) method. The muscle lipid of Cuttle fish was found to contain palmitic acid, stearic acid, linolenic acid and erucic acid respectively with some other unknown fatty acids. Antimicrobial activities of the lipid were tested by standard method and found moderate to potential antibiotic and antifungal property in this lipid. The lipid containing muscle of Cuttle fish was analyzed quantitatively for the determination of percentages of protein and minerals (N, P, K, Ca) contents by modified Kjeldahl method
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22

Al-Amin, Mohammad, M. H. Uddin, A. Afrin, K. B. Nath, and S. Barua. "Extraction, Physico-Chemical Characterization and Antimicrobial Screening of the Muscle Lipid of Cuttle Fish (<i>Sepia esculenta</i>) of the Bay of Bengal." International Letters of Chemistry, Physics and Astronomy 36 (July 15, 2014): 87–97. http://dx.doi.org/10.56431/p-933zzm.

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Lipid was extracted from the muscle of Cuttle fish by solvent extraction method and then purified by suitable conventional method. Various physical constants e.g. refractive index, viscosity, specific gravity, crude fat, crude fibre and ash contents and chemical parameters e.g. saponification value, saponification equivalent value, acid value, iodine value, acetyl value, peroxide value, thiocyanogen value, Reichert-Meissl value, Polenske value, Henher value, cholesterol content etc. of the lipid sample have been determined and compared with those of different standard oils. Fatty acids composition of the sample was investigated by Thin Layer Chromatography (TLC) method. The muscle lipid of Cuttle fish was found to contain palmitic acid, stearic acid, linolenic acid and erucic acid respectively with some other unknown fatty acids. Antimicrobial activities of the lipid were tested by standard method and found moderate to potential antibiotic and antifungal property in this lipid. The lipid containing muscle of Cuttle fish was analyzed quantitatively for the determination of percentages of protein and minerals (N, P, K, Ca) contents by modified Kjeldahl method
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23

Tajiri, Reiko. "Cuticle itself as a central and dynamic player in shaping cuticle." Current Opinion in Insect Science 19 (February 2017): 30–35. http://dx.doi.org/10.1016/j.cois.2016.10.009.

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24

Wolfgang, W. J., D. Fristrom, and J. W. Fristrom. "The pupal cuticle of Drosophila: differential ultrastructural immunolocalization of cuticle proteins." Journal of Cell Biology 102, no. 1 (January 1, 1986): 306–11. http://dx.doi.org/10.1083/jcb.102.1.306.

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Precise ultrastructural localization of Drosophila melanogaster pupal cuticle proteins (PCPs) was achieved by the immunogold labeling of frozen thin sections. PCPs were found in lamellate cuticle and intracellular vesicles but, curiously, were absent from the assembly zone of the cuticle. Antibodies that distinguish between the two classes of PCPs--low molecular weight (L-PCPs) and high molecular weight (H-PCPs)--revealed that the morphologically distinct outer lamellae contained L-PCPs and the inner lamellae contained H-PCPs. The sharp boundary between these two antigenic domains coincides with the transition from the outer to the inner lamellae, which in turn is correlated with the cessation of L-PCP synthesis and the initiation of H-PCP synthesis in response to 20-hydroxyecdysone (Doctor, J., D. Fristrom, and J.W. Fristrom, 1985, J. Cell Biol. 101:189-200). Hence, differences in protein composition are associated with differences in lamellar morphology.
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25

St. Leger, R. J., A. K. Charnley, and R. M. Cooper. "Cuticle-degrading enzymes of entomopathogenic fungi: Synthesis in culture on cuticle." Journal of Invertebrate Pathology 48, no. 1 (July 1986): 85–95. http://dx.doi.org/10.1016/0022-2011(86)90146-1.

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26

Nickerl, Julia, Mikhail Tsurkan, René Hensel, Christoph Neinhuis, and Carsten Werner. "The multi-layered protective cuticle of Collembola: a chemical analysis." Journal of The Royal Society Interface 11, no. 99 (October 6, 2014): 20140619. http://dx.doi.org/10.1098/rsif.2014.0619.

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Collembola, also known as springtails, are soil-dwelling arthropods that typically respire through the cuticle. To avoid suffocating in wet conditions, Collembola have evolved a complex, hierarchically nanostructured, cuticle surface that repels water with remarkable efficiency. In order to gain a more profound understanding of the cuticle characteristics, the chemical composition and architecture of the cuticle of Tetrodontophora bielanensis was studied. A stepwise removal of the different cuticle layers enabled controlled access to each layer that could be analysed separately by chemical spectrometry methods and electron microscopy. We found a cuticle composition that consisted of three characteristic layers, namely, a chitin-rich lamellar base structure overlaid by protein-rich nanostructures, and a lipid-rich envelope. The specific functions, composition and biological characteristics of each cuticle layer are discussed with respect to adaptations of Collembola to their soil habitat. It was found that the non-wetting characteristics base on a rather typical arthropod cuticle surface chemistry which confirms the decisive role of the cuticle topography.
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Domínguez, Eva, Gloria López-Casado, Jesús Cuartero, and Antonio Heredia. "Development of fruit cuticle in cherry tomato (Solanum lycopersicum)." Functional Plant Biology 35, no. 5 (2008): 403. http://dx.doi.org/10.1071/fp08018.

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The cuticle of a plant plays an important role in many physiological events of fruit development and ripening. Despite this, little is known about cuticle formation and development. We include a detailed morphological study at the microscopic level of cuticle during fruit growth and ripening using tomato as a fruit model. In addition, a study of the differences in cuticle thickness and composition during development is included. The four genotypes studied in this work showed a similar timing of the main morphological events: initiation of epidermal differentiation, changes in the distribution of the lipid, pectin and cellulose material within the cuticle, appearance of pegs, beginning of cuticle invaginations, maximum thickness and loss of polysaccharidic material. Fruit growth, measured by fruit diameter, showed a positive correlation with the increase of cuticle thickness and the amount of cuticle and their cutin and polysaccharide components per fruit unit during development. By contrast, cuticle waxes showed a different behaviour. Two important characteristics of cuticle growth were observed during tomato fruit development. First, the amount of cuticle per surface area reached its maximum in the first 15 days after anthesis and remained more or less constant until ripening. Second, there was a significant loss of polysaccharidic material from the beginning of ripening (breaker stage) to full red ripe.
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28

Li, Chuchu, Stanislav N. Gorb, and Hamed Rajabi. "Effect of sample treatment on the elastic modulus of locust cuticle obtained by nanoindentation." Beilstein Journal of Nanotechnology 13 (April 22, 2022): 404–10. http://dx.doi.org/10.3762/bjnano.13.33.

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Cuticle is one of the most abundant, but least studied, biological composites. As a result, it has contributed very little to the field of biomimetics. An important step to overcome this problem is to study cuticle biomechanics by means of accurate mechanical measurements. However, due to many reasons, mechanical testing on fresh cuticle specimens is not always possible. Hence, researchers often use stored specimens to measure properties of arthropod cuticle. Our knowledge about the influence of different treatment methods on cuticle properties is currently very limited. In this study, we investigated the effect of freezing, desiccation, and rehydration on the elastic modulus of the hind tibial cuticle of locusts obtained by nanoindentation. We found that all the mentioned treatments significantly influence cuticle properties. This is in contrast to previous reports suggesting that freezing did not significantly influence the elastic modulus of native cuticle specimens tested in bending. In the light of our data, we suggest that changes of the elastic modulus of cuticle are not solely due to changes of the water content. Our results provide a platform for more accurate measurements of cuticle properties.
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Skrzydeł, Joanna, Dorota Borowska-Wykręt, and Dorota Kwiatkowska. "Structure, Assembly and Function of Cuticle from Mechanical Perspective with Special Focus on Perianth." International Journal of Molecular Sciences 22, no. 8 (April 16, 2021): 4160. http://dx.doi.org/10.3390/ijms22084160.

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This review is devoted to the structure, assembly and function of cuticle. The topics are discussed from the mechanical perspective and whenever the data are available a special attention is paid to the cuticle of perianth organs, i.e., sepals, petals or tepals. The cuticle covering these organs is special in both its structure and function and some of these peculiarities are related to the cuticle mechanics. In particular, strengthening of the perianth surface is often provided by a folded cuticle that functionally resembles profiled plates, while on the surface of the petal epidermis of some plants, the cuticle is the only integral continuous layer. The perianth cuticle is distinguished also by those aspects of its mechanics and development that need further studies. In particular, more investigations are needed to explain the formation and maintenance of cuticle folding, which is typical for the perianth epidermis, and also to elucidate the mechanical properties and behavior of the perianth cuticle in situ. Gaps in our knowledge are partly due to technical problems caused by very small thicknesses of the perianth cuticle but modern tools may help to overcome these obstacles.
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KIM, Jin-Soo, Moon-LAE CHO, Min-Soo HEU, Tae-Jong CHO, Hwa-Jin AN, and Yong-Jun CHA. "Solubility Improvement of Cuttle Bone Powder Using Organic Acids." Korean Journal of Fisheries and Aquatic Sciences 36, no. 1 (February 1, 2003): 11–17. http://dx.doi.org/10.5657/kfas.2003.36.1.011.

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31

van der VEGT, Willem, and Eljakim SCHRIJVERS. "Analyzing Task Difficulty in a Bebras Contest Using Cuttle." Olympiads in Informatics 13 (July 13, 2019): 145–56. http://dx.doi.org/10.15388/ioi.2019.09.

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Predicting the difficulty level of a task on the concepts of computer science or computational thinking, like in the Bebras Challenge, proves to be really hard. But the announced difficulty level is needed in the contest format used in many local challenges. The Dutch contest system Cuttle has a new module for analysis. This is applied to one specific contest in order to find parameters explaining task difficulty. Using quantitative methods we were able to confirm a relation between answer types and difficulty and a tendency that tasks on data, data structures and representation are better answered than tasks on algorithms and programming.
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32

Mansfield, Kevin. "Christopher Cuttle (2015), Lighting Design: A Perception–Based Approach." Lighting Research & Technology 47, no. 6 (September 22, 2015): 763–64. http://dx.doi.org/10.1177/1477153515602203.

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33

Measures, Lena N., and Henry Hong. "The number of moults in the egg of sealworm, Pseudoterranova decipiens (Nematoda: Ascaridoidea): an ultrastructural study." Canadian Journal of Fisheries and Aquatic Sciences 52, S1 (August 1, 1995): 156–60. http://dx.doi.org/10.1139/f95-521.

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The number of moults in the egg of sealworm, Pseudoterranova decipiens (Nematoda: Ascaridoidea), and other ascaridoids is contentious. Transmission electron microscopic analysis of eggs and free-living larvae of sealworm confirmed that only one moult occurs in the egg. The first-stage larval (L1) cuticle on embryos was first observed in eggs incubated at 15 °C in sea water on day 5 after eggs were dissected from the uterus of sealworm obtained from the stomach of grey seals. There was no ecdysis of this L1 cuticle. A second cuticle began to form beneath the L1 cuticle between day 5 and 12. The second-stage larval (L2) cuticle continued to develop and on day 12 the L2 larva hatched enclosed within the cuticle of the first stage. The L1 cuticle appeared to be partially resorbed during development of the L2 cuticle. This study provides the first ultrastructural evidence of the number of moults that occur in eggs of sealworm.
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34

Waugh, David A., and Rodney M. Feldmann. "Cuticle microstructure as a new tool in systematic paleontology." Contributions to Zoology 72, no. 2-3 (2003): 191–93. http://dx.doi.org/10.1163/18759866-0720203025.

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Fossil decapod cuticle has received little systematic study. The purpose of the present note is to survey the cuticle architecture of eleven extant decapod crabs arrayed within ten families, and to develop a classification scheme of cuticle types suitable for describing fossil and Recent decapod cuticle morphology.
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35

Browman, David L. "Necrology: Hugh Carson Cutler." Bulletin of the History of Archaeology 9, no. 1 (May 20, 1999): 1. http://dx.doi.org/10.5334/bha.09102.

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36

Galbraith-Jones, Marian, and Kay Print Janney. "The Cutler Playwrights Festival." English Journal 76, no. 2 (February 1987): 62. http://dx.doi.org/10.2307/818172.

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37

MILL fls, ROBERT R., and DARIAN M. STARK SCHILLING. "Cuticle micromorphology ofSaxegothaea(Podocarpaceae)." Botanical Journal of the Linnean Society 159, no. 1 (January 2009): 58–67. http://dx.doi.org/10.1111/j.1095-8339.2008.00901.x.

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38

Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 7, no. 5 (September 1, 2001): 29. http://dx.doi.org/10.3142/107951401782383533.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 8, no. 3 (May 1, 2002): 27. http://dx.doi.org/10.3142/107951402782293424.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 9, no. 3 (May 1, 2003): 31–32. http://dx.doi.org/10.3142/107951403782200047.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 9, no. 4 (July 1, 2003): 29. http://dx.doi.org/10.3142/107951403782226120.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 9, no. 2 (March 1, 2003): 29. http://dx.doi.org/10.3142/107951403782226184.

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43

Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 10, no. 4 (July 1, 2004): 15. http://dx.doi.org/10.3142/107951404781540473.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 10, no. 5 (September 1, 2004): 27. http://dx.doi.org/10.3142/107951404781543821.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 11, no. 3 (May 1, 2005): 26. http://dx.doi.org/10.3142/107951405781345659.

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Kaswell, Alice Shirrell. "Icky Cutesy Research Review." Annals of Improbable Research 11, no. 5 (September 1, 2005): 26–27. http://dx.doi.org/10.3142/107951405781388436.

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47

Kaswell, Alice Shirell. "Icky Cutesy Research Review." Annals of Improbable Research 11, no. 1 (January 1, 2005): 18. http://dx.doi.org/10.3142/107951405781748166.

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48

Dirks, J. H., E. Parle, and D. Taylor. "Fatigue of insect cuticle." Journal of Experimental Biology 216, no. 10 (February 7, 2013): 1924–27. http://dx.doi.org/10.1242/jeb.083824.

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49

Lei, Lei. "Surveillance of embryonic cuticle." Nature Plants 6, no. 3 (March 2020): 179. http://dx.doi.org/10.1038/s41477-020-0627-1.

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Bradley, David. "A cuticle new composite." Materials Today 15, no. 1-2 (January 2012): 8. http://dx.doi.org/10.1016/s1369-7021(12)70004-4.

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